Abstract: Bovine herpesvirus type 1 (infectious bovine rhinotracheitis virus) mutants which fail to produce any functional thymidine kinase as a result of a deletion in the thymidine kinase gene. The deletion in the thymidine kinase gene attenuates the viruses so that they can be used as an active agent in a modified live-virus vaccine against infectious bovine rhinotracheitis. This invention also relates to methods for the production and use of the same.

Abstract: Non-A, non-B hepatitis antigen, antigen compositions, vaccine and assay test for the detection of non-A, non-B hepatitis antigen. The antigen is characterized as having a particle size of about 2.0 nm to about 5.0 nm, a density of from about 1.24 to about 1.30 g/cm.sup.3, contains ribonucleic acid and occurs either free or bound to IgG molecules.The antigen can be isolated by isopycnic banding and chromatographic fractionation, and preferably by enzymatic digestion, isopycnic banding and chromatographic fractionation.

Abstract: A liquid composition comprising a sunscreen agent, a preservative, and a non-ionic detergent, an amphoteric detergent or a mixture thereof in an aqueous vehicle. The sunscreen agent is selected from an aminobenzoic acid, the esters of an aminobenzoic acid, homosalate, anoxate, and oxybenzone and is present in an amount of not less than about 1 percent by weight of the composition. Applying the composition to the skin periodically leaves an effective amount of the sunscreen agent on the skin even after rinsing to protect the skin against sunburn.

Abstract: The present invention concerns a method for detection of the core antigen of hepatitis B virus in a sample comprising a first incubation with carrier-bound antibodies directed against hepatitis B surface antigens and a second incubation with antibodies against the core antigen in the presence of a detergent.Also claimed are test kits for carrying out this method and an immunochemical reagent comprising antibodies directed against the surface antigen and against the core antigen conjointly bound to a carrier.

Abstract: The invention relates to vaccines which, in addition to antigens, contain fat-soluble vitamins (such as vitamins A, D or E), physiologically acceptable zinc and selenium compounds and, optionally, absorption-promoting agents and/or spreading oils.

Abstract: Hydrocarbon utilizing microorganisms are immobilized on a plastic carrier in an aqueous nutrient medium to which a minor amount of a water-immiscible hydrocarbon substrate has been added. The water-immiscible hydrocarbon is preferably hexadecane or dodecylcyclohexane and the plastic carrier is preferably polytetrafluoroethylene. Presence of the water-immiscible hydrocarbon substrate results in increased loading of microorganisms on the carrier.

Abstract: A process for obtaining a sterile, apyrogenic product for promoting oxidative phosphorylation and suitable for therapeutic or cosmetic compositions, starting from yeast, in which any type of yeast is subjected to a process of plasmolysis, followed by treatment with proteolytic enzymes and then with diamine oxidase, after which the proteins present in the solution are precipitated by alcohols, the solution pH is stabilized, and the solution concentrated at low temperature under vacuum.

Abstract: An agent for quantitative determination of microorganisms, which comprises(a) an antibody prepared from a strain of the same species as the species of microorganisms to be determined,(b) an insolubilized cell component of a strain which can sufficiently bind to the antibody (a) but does not release and replace to other strains which have been bound with the antibody (a), and(c) a labelled second antibody, and a method for the quantitative determination of microorganisms. The agent and method of the present invention are useful for determinating simultaneously not only a strain from which the antibody is prepared but also other strains of the same species which are contained in the test sample.

Abstract: A method for rapid detection of microorganisms in a body fluid sample includes detecting the microorganisms after treatment of the sample with a lysing agent in order to dissolve sample components other than microorganisms, and staining with a fluorescent dye. The lysing agent may be part of a composition which includes a culture medium thereby providing simultaneous lysis and growth before staining. Detection is preferably accomplished by reliance on the fluorescence emitted by the dye having been properly excited by light energy.A composition suitable for use in the above-described method includes a growth medium and a lysing agent.

Abstract: Apparatus and methods for selective removal of specific biological cells or specific antigens or antibodies, from fluid containing their mixture with other biological cells and particulates are described. Filtration by this biochemical filter system is effected in a continuous closed-loop fluid flow path. The apparatus described herein comprises a source of fluid containing specific biological cells, antigens or antibodies to be removed; a source of complementary cells or complementary antibodies for the antigens and complementary antigens for the antibodies which can form large agglutinates following a biochemical reaction a reaction chamber providing conditions favorable for fast clump formation following the reaction; a filter for trapping large agglutinates; one or more pumps to regulate various flow rates; and necessary connecting links to form a closed-loop fluid flow path that includes the sources of biological cells, antigens or antibodies and complementary cells, reaction chamber, and filter chamber.

Abstract: Compositions, devices, and methods are disclosed for controlling insect activity wherein an insect control agent is self-adhered to a rough-surfaced fiber. Preferably the fiber is a graft polymer of cellulose and an ethylenically unsaturated material copolymerizable with cellulose. The invention provides extended control of insect activity.

Abstract: A feed liquid (sugar-containing liquid) is continuously fed to a fermenter packed with an immobilized microorganism, which is obtained by immobilizing an alcohol producing microorganism on a carrier, for alcohol fermentation. The fermentation liquid is continuously taken out of said fermenter and is heated to a temperature of 35.degree. to 80.degree. C. This liquid is introduced into a flash tank maintained at reduced pressure and is divided into an alcohol-containing steam and a liquid. The alcohol-containing steam is condensed and recovered as alcohol, while the separated liquid is cooled and is cycled back to said fermenter.

Abstract: Method for the performance of a mutagenicity test so that a cell population is subjected to a mutagen. As a result of this, a part of the population mutates, whereat the originally uniform cell population is differentiated into sub-populations. The variation of turbidity caused by the increase in the density of the differentiated cell populations is measured as a change in optical density by means of a photometer measuring vertically at a certain wavelength, and the quantity of cells in the sub-populations is determined as optical density. The absorbance values are measured in accordance with a pre-programmed time share system and a growth curve is formed out of the values as a function of time.

Abstract: The invention relates to a highly immunogenic virus preparation derived by attenuation employing serial passages in avian cell cultures and six plaque purification steps from a Marek's disease virus strain, belonging to Avian Herpesvirus serotype-1. The original virus strain MDV CVI-988 was recovered from a healthy chicken (Avian Diseases 16: 108-125, 1972).The live purified Marek's disease vaccine so obtained is innocuous for specific pathogen free Rhode Island Red chickens which are highly susceptible for development of Marek's disease tumors. The monovalent virus preparation, according to the invention, demonstrated in comparison with Marek's disease vaccines based on the same strain, currently in use at a lower cell passage level, an improved protective efficacy against challenge infection by two representatives of virulent Marek's disease virus.

Abstract: The process consists in treating the crop conjointly with at least one baculovirus from Spodoptera littoralis or Mamestra brassicae and at least one photostable pyrethrinoid.The insecticidal compositions comprises the combination of at least one of the said baculovirus and at least one photostable pyrethrinoid; doses of baculovirus and pyrethrinoid are advantageously below usual doses.Such compositions and process are used to control noctuid Lepidoptera by potentiation of the viroses induced by nuclear polyhedra.

Abstract: A method for production of an immobilized enzyme by means of a crosslinking agent wherein the following components are brought together in aqueous medium:(a) an enzyme preparation;(b) a crosslinking agent; and(c) an inert water soluble salt in a relatively high concentration.The salt hinders solubility of the enzyme in the medium, yet the enzyme is fully accessible to the crosslinking agent. High enzyme activity recovery may be obtained.

Abstract: The invention relates to a device and method for performing qualitative enzyme immunoassays. The device comprises at least one tube having antibody, antigen or hapten attached to an internal surface thereof. A first syringe is connectable to a first end of the tube or tubes and supplies a flow of test liquid therethrough. A second syringe having a two piston arrangement is connected to the tube so as to allow a first wash solution followed by an enzyme conjugate solution to be flowed therethrough. Finally a third syringe, also having a two piston configuration, is connected to the tube so as to allow a second wash solution followed by an enzyme substrate and metabolite indicator solution to be flowed therethrough.

Abstract: A cosmetic make-up composition comprises, in combination, cosmetic make-up components and, as a binding agent therefor, an intimate mixture of from 5 to 95 percent by weight of said mixture of finely divided silica and about 95 to about 5 percent by weight of said mixture of finely divided polyethylene fibers having a fibrillar structure.

Abstract: There is provided an assay method for determining the concentration of magnesium ions in biological fluids. The method comprises the introduction of a sample of biological fluid to a magnesium-free assay solution containing at least one reactant and an enzyme capable of catalyzing a reaction with at least one reactant wherein the enzyme activity, and hence the rate of reaction, is partially or totally dependent on and correlatable to the concentration of magnesium that is present. The rate of reaction is measured by monitoring the loss of a detectable reactant or the formation of a detectable product.

Abstract: A cosmetic suitable for application to the skin (such as eye-shadow, rouge and pressed face powder) is formed by mixing differently colored pigment compositions with binders to form differently colored grains, intermixing the grains and compression-moulding them into a compact, but without destroying the grain structure thereof. The differently colored grains may then separately or simultaneously be applied to the skin with a puff.