Patents Examined by Sheridan Swope
  • Thermostable protease variants
    Patent number: 10138473
    Abstract: The present invention relates to proteases having at least 75% identity to a protease derived from Thermoascus aurantiacus and comprises at least one modification in the amino acid sequence thereof. These protease variants have improved thermostability. The invention also relates to DNA encoding these proteases, methods of their production, as well as the use thereof.
    Type: Grant
    Filed: April 20, 2015
    Date of Patent: November 27, 2018
    Assignee: NOVOZYMES A/S
    Inventors: Tomoko Matsui, Allan Noergaard, Thomas Agersten Poulsen, John Matthews
  • Recombinant cellular iysate system for producing a product of interest
    Patent number: 10036001
    Abstract: The embodiments described herein pertain to cells, and methods for preparing cells, that can be used as biocatalysts by altering enzymes that compete for a substrate or product of a pathway of interest such that the targeted enzyme is sensitive to a site-specific protease, which protease is expressed but relocated in the cell to a site where it is not in contact with the targeted enzyme in the intact cell. Upon cell lysis, the protease contacts the target enzyme, which is then inactivated by protease cleavage.
    Type: Grant
    Filed: November 14, 2014
    Date of Patent: July 31, 2018
    Assignees: The Board of Trustees of the Leland Stanford Junior University, GreenLight Biosciences, Inc.
    Inventor: James R. Swartz
  • Reducing adhesion of bacteria to a surface or releasing bacteria from a surface to which they adhere using endo-beta-A,4-glucanases
    Patent number: 10005988
    Abstract: The present invention provides a method for reducing adhesion of bacteria to a surface, or releasing the bacteria from a surface to which they adhere, using an endo-beta-1,4-glucanase.
    Type: Grant
    Filed: October 3, 2013
    Date of Patent: June 26, 2018
    Assignee: NOVOZYMES A/S
    Inventors: Klaus Gori, Lillian Eva Tang Baltsen, Marie Allesen-Holm
  • Animal feed additives comprising a protease and methods of using
    Patent number: 10006017
    Abstract: The present invention relates to isolated polypeptides having protease activity and isolated nucleic acid sequences encoding the proteases. The invention also relates to nucleic acid constructs, vectors, and host cells, including plant and animal cells, comprising the nucleic acid sequences, as well as methods for producing and using the proteases, in particular the use of the proteases in animal feed.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: June 26, 2018
    Assignee: Novozymes A/S
    Inventors: Tine Hoff, Peter Rahbek Oestergaard, Katrine Fruergaard Pontoppidan
  • Methods for cyclic nucleotide determination
    Patent number: 10000792
    Abstract: The present invention relates in general to cellular analysis tools and more particularly to methods for detecting or determining cyclic nucleotide concentrations in samples. Samples containing cyclic nucleotides may be contacted with a cyclic nucleotide-dependent protein kinase and a detection system which includes a substrate for the cyclic nucleotide-dependent protein kinase. The activities in cyclic nucleotide related pathways may be measured using the detection system.
    Type: Grant
    Filed: February 22, 2010
    Date of Patent: June 19, 2018
    Assignee: PROMEGA CORPORATION
    Inventors: Said A. Goueli, Kuei-Hsuan Hsiao, Meera Kumar, Jolanta Vidugiriene
  • Polypeptides having protease activity and polynucleotides encoding same
    Patent number: 9994838
    Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: March 17, 2017
    Date of Patent: June 12, 2018
    Assignee: Novozymes A/S
    Inventors: Martin Simon Borchert, Jeppe Wegener Tams
  • Thermolysin variants
    Patent number: 9976134
    Abstract: The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation.
    Type: Grant
    Filed: July 8, 2015
    Date of Patent: May 22, 2018
    Assignee: DANISCO US INC.
    Inventors: David A. Estell, Ronaldus W. J. Hommes, Amy D. Liu, Andrew Shaw
  • Method for making mutated pullulanase enzyme, mutated pullulanase enzyme, and microorganism expressing the same
    Patent number: 9969996
    Abstract: A method for making a mutated pullulanase enzyme that hydrolyzes an ?-1,6-glycosidic linkage is provided. The method includes obtaining the amino acid sequence of a pullulanase enzyme having an amino acid sequence that is at least 95% identical to the amino acid sequence set forth in SEQ ID NO: 13; identifying an amino acid to be mutated in the pullulanase enzyme of step (1), wherein the amino acid to be mutated corresponds to the amino acid at position Phe476 of SEQ ID NO: 2; constructing a mutated amino acid sequence by substituting the amino acid to be mutated with another amino acid or deleting the amino acid to be mutated, thereby making a mutated pullulanase enzyme having the mutated amino acid sequence that has increased affinity for pullulan and that hydrolyzes an ?-1,6-glycosidic linkage.
    Type: Grant
    Filed: December 29, 2015
    Date of Patent: May 15, 2018
    Assignee: AMANO ENZYME INC.
    Inventors: Bunzo Mikami, Hiroyuki Iwamoto, Shotaro Yamaguchi
  • Thermostable cellobiohydrolase
    Patent number: 9963692
    Abstract: The thermostable cellobiohydrolase of the present invention is a polypeptide which has cellobiohydrolase activity at least under conditions of a temperature of 75° C. and a pH of 5.5, and which includes a polypeptide including an amino acid sequence represented by SEQ ID NO: 1, 3, 5, or 7, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in an amino acid sequence represented by SEQ ID NO: 1, 3, 5, or 7, or a polypeptide including an amino acid sequence having 80% or greater but less than 100% sequence identity with an amino acid sequence represented by SEQ ID NO: 1, 3, 5, or 7.
    Type: Grant
    Filed: March 27, 2014
    Date of Patent: May 8, 2018
    Assignees: Honda Motor Co., Ltd., Kazusa DNA Research Institute
    Inventors: Migiwa Suda, Jiro Ohkuma, Asuka Yamaguchi, Yoshitsugu Hirose, Yasuhiro Kondo, Tomohiko Kato, Daisuke Shibata
  • Penicillin-G acylases
    Patent number: 9944916
    Abstract: The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
    Type: Grant
    Filed: May 6, 2016
    Date of Patent: April 17, 2018
    Assignee: Codexis, Inc.
    Inventors: Goutami Banerjee, Jie Yang, Xiyun Zhang, Erika M. Milczek, Melissa Ann Mayo, Stephan Jenne
  • Methods for treating glycogen storage disease type IV usng lysosomal acid alpha-glucosidase
    Patent number: 9850474
    Abstract: The disclosure relates, in general, to Glycogen Storage Disease and, in particular, to a method of treating Glycogen Storage Disease and to compounds and compositions suitable for use in such a method.
    Type: Grant
    Filed: April 30, 2015
    Date of Patent: December 26, 2017
    Assignee: Duke University
    Inventors: Yuan-Tsong Chen, Priya Kishnani, Baodong Sun
  • Modified non-cytotoxic proteases
    Patent number: 9849163
    Abstract: The present invention relates to a modified polypeptide comprising a non-cytotoxic protease, a translocation domain, a destructive protease cleavage site and a Targeting Moiety that binds to a Binding Site on a nerve cell, wherein after cleavage of the destructive cleavage site the polypeptide has reduced potency. The destructive cleavage site is recognized and cleaved by a protease present at or in an off-site target cell, and, in one embodiment, the polypeptide is a modified clostridial neurotoxin. The present invention also relates to the use of said polypeptides for treating a range of conditions, and to nucleic acids encoding said polypeptides.
    Type: Grant
    Filed: December 16, 2009
    Date of Patent: December 26, 2017
    Assignee: Ipsen Bioinnovation Limited
    Inventors: John Andrew Chaddock, Keith Alan Foster
  • Methods of treatment of collagen-mediated diseases and conditions
    Patent number: 9833498
    Abstract: Methods and combinations are provided for controlling the duration of action, in vivo, of matrix-degrading enzymes. The methods and combinations permit temporary in-vivo activation of matrix-degrading enzymes upon administration to the extra cellular matrix (or “ECM”). Matrix-degrading enzymes having a controlled duration of action can be used to treat ECM-mediated diseases or disorders characterized by increased deposition or accumulation of one or more ECM components.
    Type: Grant
    Filed: March 6, 2009
    Date of Patent: December 5, 2017
    Assignee: Halozyme, Inc.
    Inventors: Gilbert Keller, Gregory I. Frost
  • Method for producing an L-glutamic acid and L-aspartic acid using a recombinant microorganism having enhanced expression of a ybjL protein
    Patent number: 9822385
    Abstract: An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.
    Type: Grant
    Filed: May 28, 2015
    Date of Patent: November 21, 2017
    Assignee: AJINOMOTO CO., INC.
    Inventors: Yoshihiko Hara, Keita Fukui, Yoshinori Tajima, Kazue Kawamura, Yoshihiro Usuda, Kazuhiko Matsui
  • Administration of kynurenine depleting enzymes for tumor therapy
    Patent number: 9808486
    Abstract: Methods and compositions related to the use of a protein with kynureninase activity are described. For example, in certain aspects there may be disclosed a modified kynureninase capable of degrading kynurenine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with kynurenine depletion using the disclosed proteins or nucleic acids.
    Type: Grant
    Filed: August 29, 2014
    Date of Patent: November 7, 2017
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: George Georgiou, Everett Stone
  • Detergent compositions comprising lipase
    Patent number: 9809787
    Abstract: The present invention relates a method of obtaining a detergent composition comprising introducing (a) a lipase variant of a parent lipase which variant has at least 60% sequence identity with SEQ ID NO: 2, a substitution at a position corresponding to D254 of the mature polypeptide of SEQ ID NO: 2 and has lipase activity and (b) an anionic surfactant, wherein said composition has increased stability in comparison with a corresponding composition comprising the parent lipase.
    Type: Grant
    Filed: December 20, 2012
    Date of Patent: November 7, 2017
    Assignee: NOVOZYMES A/S
    Inventors: Jesper Vind, Lise Munch Mikkelsen, Marco Malten, Allan Svendsen, Kim Borch
  • Use of polypeptides having protease activity in animal feed and detergents
    Patent number: 9801398
    Abstract: The present invention relates to the use of isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides in animal feed and detergents. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides in e.g. animal feed and detergents.
    Type: Grant
    Filed: January 25, 2013
    Date of Patent: October 31, 2017
    Assignee: Novozymes A/S
    Inventors: Robert Piotr Olinski, Tine Hoff, Peter Rahbek Oestergaard, Carsten Sjoeholm, Katrine Pontoppidan
  • Modified MT-SP1 proteases that inhibit complement activation
    Patent number: 9795655
    Abstract: Provided are methods for and compounds for modulating the complement system. In particular, compounds are provided that inhibit complement activation and compounds are provided that promote complement activation. The compounds are therapeutics by virtue of their effects on the complement system. Hence, the compounds that inhibit complement activation can be used for treatment of ischemic and reperfusion disorders, including myocardial infarction and stroke, sepsis, autoimmune diseases, inflammatory diseases and diseases with an inflammatory component, including Alzheimer's Disease and other neurodegenerative disorders.
    Type: Grant
    Filed: May 12, 2014
    Date of Patent: October 24, 2017
    Assignee: Catalyst Biosciences, Inc.
    Inventors: Edwin L. Madison, Jack Nguyen, Sandra Waugh Ruggles, Christopher Thanos
  • Methods of treatment of cellulite
    Patent number: 9775889
    Abstract: Methods and combinations are provided for controlling the duration of action, in vivo, of matrix-degrading enzymes. The methods and combinations permit temporary in-vivo activation of matrix-degrading enzymes upon administration to the extra cellular matrix (or “ECM”). Matrix-degrading enzymes having a controlled duration of action can be used to treat ECM-mediated diseases or disorders characterized by increased deposition or accumulation of one or more ECM components.
    Type: Grant
    Filed: April 2, 2014
    Date of Patent: October 3, 2017
    Assignee: Halozyme, Inc.
    Inventors: Gilbert Keller, Gregory I. Frost
  • Polypeptides having protease activity
    Patent number: 9771570
    Abstract: The present invention relates to isolated polypeptides having protease activity and isolated nucleic acid sequences encoding the proteases. The invention also relates to nucleic acid constructs, vectors, and host cells, including plant and animal cells, comprising the nucleic acid sequences, as well as methods for producing and using the proteases, in particular the use of the proteases in animal feed.
    Type: Grant
    Filed: September 5, 2013
    Date of Patent: September 26, 2017
    Assignee: Novozymes A/S
    Inventors: Mary Ann Stringer, Tine Hoff, Peter Rahbek Oestergaard, Katrine Pontoppidan