Abstract: Some embodiments of the systems, methods and compositions provided herein relate to a compound protease. In some embodiments, the compound protease includes a protease domain and a cut site for another enzyme. In some embodiments, the compound protease includes an association domain. In some embodiments, the compound protease is part of a protein circuit.
Type:
Grant
Filed:
January 17, 2019
Date of Patent:
January 26, 2021
Assignee:
California Institute of Technology
Inventors:
Xiaojing Gao, Lucy S. Chong, Michael Elowitz, Mark William Budde, Matthew Sun-min Kim
Abstract: This invention relates to fabric and home care products comprising one or more cold water proteases and processes for making and using such products. Such compositions provide improved cleaning and freshness. Such cold water proteases may be derived from parent enzymes, including BPN? subtilisin and subtilisin derived from Bacillus lentus, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.
Type:
Grant
Filed:
December 13, 2017
Date of Patent:
January 26, 2021
Assignee:
The Procter & Gamble Company
Inventors:
Philip Frank Souter, Glenn Steven Ward, Viktor Yuryevich Alekseyev, Joshua Roy Basler, Luis Gustavo Cascao-Pereira, David A Estell, Ayrookaran J Poulose, James T Kellis, Jr., Alexander Pisarchik, Daniel Torres-Pazmino
Abstract: Formulations, compositions and products for reducing allergenic Fel d 1 from the environment are disclosed. The formulations include at least one protease that interacts with the Fel d 1 and substantially degrades allergenic epitopes on the Fel d 1. Methods of using the formulations for reducing or eliminating allergenic Fel d 1 from the environment are also disclosed.
Type:
Grant
Filed:
April 4, 2018
Date of Patent:
January 12, 2021
Assignee:
SOCIÉTÉ DES PRODUITS NESTLÉ S.A.
Inventors:
George Wells, Delphine Tissot-Favre, Ebenezer Satyaraj, Juergen Eck, Daniel Meyer, Torsten Ertongur-Fauth, Alexander Pelzer, Scott Sherrill, Peichuan Sun
Abstract: The present disclosure relates to proteases comprising an amino acid sequence which has at least about 70% sequence identity to the amino acid sequence indicated in SEQ ID NO. 1, over the entire length thereof, and in which the amino acids in the positions corresponding to positions 24, 33, 53, 78, 101, 128 and 217 according to SEQ ID NO. 1 are substituted at 24G, 33T, 53G, 78N, 101N, 128A and 217Q, and to the production and use thereof. Said type of proteases have a very good cleaning performance compared to reference proteases.
Abstract: The present invention relates to acetyl transferases, nucleic acid sequences coding therefore, expression constructs and vectors comprising these sequences, microorganisms transformed therewith and use thereof.
Type:
Grant
Filed:
October 4, 2019
Date of Patent:
December 15, 2020
Assignee:
DSM IP ASSETS B.V.
Inventors:
Christopher Farrell, Peter Houston, Lisa Laprade, Nathalie Balch, Maria Mayorga
Abstract: Process for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulfur. The microorganism and/or the culture medium are modified in such way that the methionine/carbon source yield is increased. The isolation of methionine or its derivates from the fermentation medium is also described.
Type:
Grant
Filed:
February 12, 2016
Date of Patent:
December 8, 2020
Assignee:
EVONIK DEGUSSA GMBH
Inventors:
Rainer Figge, Philippe Soucaille, Guillaume Barbier, Gwénaëlle Bestel-Corre, Cédric Boisart, Michel Chateau
Abstract: The present invention relates to cofactor regeneration systems, components and uses thereof and methods for generating and regenerating cofactors. The cofactor regeneration system comprises a first electron transfer component selected from a polypeptide comprising a NADH:acceptor oxido-reductase or NADPH:acceptor oxido-reductase, a second electron transfer component selected from a hydrogenase moiety and/or non-biological nanoparticles and an electronically conducting surface. The first and second electron transfer components are immobilised on the electrically conducting surface, and the first and second electron transfer components do not occur together in nature as an enzyme complex.
Type:
Grant
Filed:
October 3, 2012
Date of Patent:
October 13, 2020
Assignees:
OXFORD UNIVERSITY INNOVATION LIMITED, HUMBOLDT-UNIVERSITAT ZU BERLIN
Inventors:
Kylie Vincent, Lars Lauterbach, Oliver Lenz
Abstract: A system for the identification of proteases and protease inhibitors is provided. The system has at least two components. The first component is a reporter construct with at least one binding site, a transcriptional promoter, an inducible promoter region, and at least one reporter gene, all functionally connected for expression of the reporter gene(s) in functional coordination with a transcriptional activation agent. The second component is a transcriptional activation agent comprising a nucleic acid binding domain, at least one protease substrate domain, and at least one transcriptional activation domain for an inducible promoter. The system allows detection and evaluation of agents affecting protease activity directed to the protease substrate domain. The system also allows for the detection of the presence of proteases in environmental samples.
Type:
Grant
Filed:
September 13, 2013
Date of Patent:
September 29, 2020
Assignee:
IPSEN PHARMA S.A.S.
Inventors:
George A. Oyler, Yung-Nien Chang, Yien Che Tsai
Abstract: The present application demonstrates that sirtuin 4 (SIRT4) acts as a cellular lipoamidase that negatively regulates pyruvate dehydrogenase complex (PDHC) activity through hydrolysis of its lipoamide cofactors.
Type:
Grant
Filed:
January 15, 2015
Date of Patent:
September 29, 2020
Assignee:
THE TRUSTEES OF PRINCETON UNIVERSITY
Inventors:
Thomas Shenk, Todd M. Greco, Ileana M. Cristea, Rommel A. Mathias, Adam Oberstein
Abstract: The present disclosure relates to engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.
Type:
Grant
Filed:
July 29, 2019
Date of Patent:
September 22, 2020
Assignee:
Codexis, Inc.
Inventors:
Goutami Banerjee, Jie Yang, Xiyun Zhang, Erika M. Milczek, Melissa Ann Mayo, Stephan Jenne
Abstract: The present invention relates in general to cellular analysis tools and more particularly to methods and systems for detecting or determining cyclic nucleotide concentrations and related pathway activation in samples. Samples containing cyclic nucleotides may be contacted with a cyclic nucleotide-dependent protein kinase (e.g., cAMP- and cGMP-dependent protein kinases) and a detection system that includes a substrate capable of being phosphorylated by the cyclic nucleotide-dependent protein kinase. The activity of cyclic nucleotide related cell signaling pathways may be measured based on detecting the activity of the cyclic nucleotide-dependent protein kinase.
Type:
Grant
Filed:
June 7, 2018
Date of Patent:
September 22, 2020
Assignee:
PROMEGA CORPORATION
Inventors:
Said A. Goueli, Kuei-Hsuan Hsiao, Meera Kumar, Jolanta Vidugiriene
Abstract: A liquid or dried granulated milk clotting aspartic protease enzyme composition and process for isolating a milk clotting aspartic protease enzyme of interest.
Type:
Grant
Filed:
July 11, 2014
Date of Patent:
September 22, 2020
Assignee:
CHR. HANSEN A/S
Inventors:
Martin Lund, Jonas Jacobsen, Johannes Maarten Van Den Brink
Abstract: The presently-disclosed subject matter includes isolated polypeptides that comprise a butyrylcholinestrase (BChE) polypeptide and a second polypeptide. The BChE polypeptide as well as the second polypeptide can be variants and/or fragments thereof. The presently-disclosed subject matter also includes a pharmaceutical composition that comprises the present isolated polypeptide and a suitable pharmaceutical carrier. Further still, methods are provided for treating cocaine-induced conditions, and comprise administering the isolated polypeptide and/or pharmaceutical compositions thereof to an individual.
Type:
Grant
Filed:
December 11, 2013
Date of Patent:
September 15, 2020
Assignee:
University of Kentucky Research Foundation
Inventors:
Chang-Guo Zhan, Fang Zheng, Hsin-Hsiung Tai, Xiabin Chen, Liu Xue, Shurong Hou
Abstract: Methods and compositions related to the use of a protein with kynureninase activity are described. For example, in certain aspects there may be disclosed a modified kynureninase capable of degrading kynurenine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with kynurenine depletion using the disclosed proteins or nucleic acids.
Type:
Grant
Filed:
April 2, 2019
Date of Patent:
September 15, 2020
Assignee:
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
Abstract: The present invention relates to a novel polypeptide which displays IgG cysteine protease activity, and in vivo and ex vivo uses thereof. Uses of the polypeptide include methods for the prevention or treatment of diseases and conditions mediated by IgG, and methods for the analysis of IgG.
Type:
Grant
Filed:
February 12, 2016
Date of Patent:
September 1, 2020
Assignee:
HANSA BIOPHARMA AB
Inventors:
Christian Kjellman, Sofia Jarnum, Emma Nordahl
Abstract: The present invention relates to a modified polypeptide comprising a non-cytotoxic protease, a translocation domain, a destructive protease cleavage site and a Targeting Moiety that binds to a Binding Site on a nerve cell, wherein after cleavage of the destructive cleavage site the polypeptide has reduced potency. The destructive cleavage site is recognised and cleaved by a protease present at or in an off-site target cell, and, in one embodiment, the polypeptide is a modified clostridial neurotoxin. The present invention also relates to the use of said polypeptides for treating a range of conditions, and to nucleic acids encoding said polypeptides.
Type:
Grant
Filed:
April 28, 2016
Date of Patent:
August 18, 2020
Assignee:
IPSEN BIOINNOVATION LIMITED
Inventors:
John Andrew Chaddock, Keith Alan Foster
Abstract: Methods for producing a trans-splicing intein-modified protease with enhanced solubility and regulating its activity are described. Intein-modified proteases having enhanced solubility and polynucleotides encoding the same are provided. Methods of storing trans-splicing proteases are also described.
Type:
Grant
Filed:
October 28, 2015
Date of Patent:
August 4, 2020
Assignee:
AGRIVIDA, INC.
Inventors:
R. Michael Raab, Binzhang Shen, Gabor Lazar
Abstract: The present invention relates to isolated polypeptides with lipase activity. The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.
Type:
Grant
Filed:
November 8, 2018
Date of Patent:
July 21, 2020
Assignee:
NOVOZYMES A/S
Inventors:
Ming Li, Tianqi Sun, Xianshi Jiang, Lone Baunsgaard, Carsten Hoerslev Hansen, Kim Borch, Han Bucong
Abstract: Isolated polypeptides with lipase activity; polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides for cleaning are disclosed.
Type:
Grant
Filed:
February 5, 2019
Date of Patent:
June 9, 2020
Assignee:
NOVOZYMES A/S
Inventors:
Ming Li, Tianqi Sun, Xianzhi Jiang, Lone Baunsgaard, Carsten Hoerslev Hansen, Kim Borch, Han Bucong