Patents Examined by Sheridan Swope
  • Liquid washing or cleaning agent containing protease and amylase
    Patent number: 9752102
    Abstract: The intention is to improve storage stability in a liquid washing or cleaning agent which contains a protease and amylase. This is achieved by using a protease which comprises an amino acid sequence which, over the entire length thereof, is at least 70% identical to the amino acid sequence stated in SEQ ID no. 1 and, in the numbering according to SEQ ID no. 1, has the amino acid substitution R99E or R99D in combination with at least two further amino acid substitutions which are selected from the group consisting of S3T, V4I and V199I.
    Type: Grant
    Filed: June 12, 2014
    Date of Patent: September 5, 2017
    Assignee: HENKEL AG & CO. KGAA
    Inventors: Nina Mussmann, Thomas Eiting, Thorsten Bastigkeit, Konstantin Benda, Hendrik Hellmuth
  • Polynucleotide encoding psicose epimerase and method of producing psicose using the same
    Patent number: 9701953
    Abstract: A psicose 3-epimerase, a polynucleotide encoding the enzyme, a recombinant vector carrying the polynucleotide, a recombinant cell harboring the recombinant vector, and use thereof are provided.
    Type: Grant
    Filed: July 4, 2014
    Date of Patent: July 11, 2017
    Assignee: SAMYANG CORPORATION
    Inventors: Eun Jin Han, Hye Jung Kim, Sin Hye Ahn, Se-Hui Jeon, Chong Jin Park, Kang Pyo Lee
  • Method of analyzing L-tryptophan in biological samples, and kit used therein
    Patent number: 9695460
    Abstract: Disclosed is a method for quantifying L-tryptophan involving a step for mixing a specimen, L-tryptophan oxidase, and water, a step for allowing the obtained reaction solution to stand a predetermined period of time in the presence of oxygen, and a step for measuring the reaction product resulting from action of enzymes present in the reaction solution after allowing to stand. Also disclosed are a kit used to quantify the L-tryptophan containing L-tryptophan oxidase, and an enzyme sensor using the L-tryptophan oxidase. This method, kit and enzyme sensor use an L-tryptophan-specific enzyme, so are capable of quantifying L-tryptophan even in the presence of other amino acids.
    Type: Grant
    Filed: September 3, 2013
    Date of Patent: July 4, 2017
    Assignees: Public University Corporation Toyama Prefectural University, Ajinomoto Co., Inc.
    Inventors: Yasuhisa Asano, Masafumi Kameya, Hiroyasu Onaka
  • Engineered phosphoglucose isomerase proteins with a protease cleavage site
    Patent number: 9688977
    Abstract: Various aspects and embodiments herein relate to recombinant proteins with at least one protease recognition sequence that can be inactivated by a cognate protease and methods of preparing such proteins. In some embodiments, recombinant phosphoglucose isomerase (Pgi) proteins are provided. In other embodiments, recombinant phosphotransacetylase (Pta) proteins are provided. In yet other embodiments, recombinant transketolase A (TktA) proteins are provided.
    Type: Grant
    Filed: August 5, 2014
    Date of Patent: June 27, 2017
    Assignee: GreenLight Biosciences, Inc.
    Inventors: William Jeremy Blake, Drew S. Cunningham
  • Method for producing a recombinant protein of interest by using the Ntechnology
    Patent number: 9677107
    Abstract: Disclosed is a method for producing a recombinant protein of interest, the method being characterized in by the following steps: (a) providing a fusion protein comprising an Npro autoprotease moiety and a protein of interest moiety in inclusion bodies, (b) solubilizing the inclusion bodies, (c) allowing the fusion protein to be cleaved by the Npro autoprotease moiety under chaotropic conditions, wherein the recombinant protein of interest is cleaved from the fusion protein and wherein the recombinant protein of interest is not yet renatured or simultaneously renatured, and (d) recovering the protein of interest, optionally including a renaturing step for the protein of interest.
    Type: Grant
    Filed: December 19, 2013
    Date of Patent: June 13, 2017
    Assignees: SANDOZ AG, BOEHRINGER INGELHEIM RCV GMBH & CO KG
    Inventors: Maria Reitmeir, Rainer Schneider, Bernhard Auer
  • Methods of treatment of cancer using SRC-modulating peptides
    Patent number: 9663561
    Abstract: A method for regulating Src and its downstream signaling pathway which includes binding between Src and Na+/K+-ATPase is disclosed. The Na+/K+-ATPase/Src complex is a functional receptor for cardiotonic steroids such as ouabain. Also disclosed are Src inhibitors or activators which include either Na+/K+-ATPase or Src that interfere with the interaction between the Na/K-ATPase and Src, act via a different mechanism from ATP analogues, and is pathway (Na+/K+-ATPase) specific. Also disclosed is use of said Src-modulating peptides for treatment of cancer.
    Type: Grant
    Filed: January 28, 2015
    Date of Patent: May 30, 2017
    Assignee: The University Of Toledo
    Inventors: Zi-Jian Xie, Joseph I. Shapiro, Jiang Tian, Zhichuan Li
  • Polypeptide cleavage method using OmpT protease variant
    Patent number: 9657323
    Abstract: A polypeptide cleavage method characterized in that arginine or lysine is at the P1 position of a desired cleavage site in a polypeptide, an amino acid other than aspartic acid, glutamic acid or proline is at the P1? position, a single basic amino acid or two or three consecutive basic amino acids are situated at any site in the amino acid sequence from the P10 position to the P3 position or from the P3? position to the P5? position (with the proviso that a single basic amino acid is not situated at the P6 or P4 position), and OmpT protease or its variant enzyme having a substitution at the 97th amino acid from the N-terminus is used to cleave the desired cleavage site in the polypeptide.
    Type: Grant
    Filed: September 29, 2004
    Date of Patent: May 23, 2017
    Assignee: DAIICHI SANKYO COMPANY, LIMITED
    Inventors: Kazuaki Okuno, Masayuki Yabuta
  • Improving the solubility of an alkaline protease in a liquid detergent by amino acid substitution
    Patent number: 9650623
    Abstract: Providing an alkaline protease exhibiting an improved solubility in a liquid detergent. A mutant alkaline protease consisting of the amino acid sequence represented by SEQ ID No: 2 or an amino acid sequence having 80% or more sequence identity therewith, in which at least one amino acid residue selected from the group consisting of the amino acid residues at predetermined positions of the amino acid sequence represented by SEQ ID No: 2 or corresponding positions thereto are substituted.
    Type: Grant
    Filed: April 9, 2013
    Date of Patent: May 16, 2017
    Assignee: Kao Corporation
    Inventors: Masatoshi Tohata, Yumi Nishimura, Yasunao Wada, Katsuhisa Saeki, Mitsuyoshi Okuda
  • Cold-adapted protease derived from PAMC 21717 and uses thereof
    Patent number: 9644194
    Abstract: A cold-adapted protease derived from Pseudoalteromonas arctica PAMC 21717 or a recombinant cold-adapted protease obtained by expressing a gene encoding the cold-adapted protease in E. coli is described, and more particularly, a crystal of a protease exhibiting activity at low temperatures, a method for crystallizing the protease, a method for preparing the protease, a recombinant microorganism that expresses the protease, a method for preparing the recombinant microorganism, a method for preparing the recombinant protease and the use of the cold-adapted protease. The cold-adapted protease exhibits high activity at low temperatures, and securely maintains its enzymatic activity even in the presence of high pH, various metal ions and surfactants. Thus, it is useful in various industrial applications.
    Type: Grant
    Filed: December 26, 2014
    Date of Patent: May 9, 2017
    Assignee: KOREA INSTITUTE OF OCEAN SCIENCE & TECHNOLOGY
    Inventors: Joung Han Yim, Bon-Hun Koo, Chul Soo Shin, Dockyu Kim, Il-Chan Kim, Se Jong Han, Jun Hyuck Lee, Ha Ju Park
  • Mutant luciferases
    Patent number: 9587266
    Abstract: Described are mutant luciferases, nucleic acids that encode them, cells and animals expressing them, methods of use thereof, and kits.
    Type: Grant
    Filed: February 9, 2012
    Date of Patent: March 7, 2017
    Assignee: University of Massachusetts
    Inventors: Stephen C. Miller, David Mofford, Katryn Harwood
  • Protease-deficient
    Patent number: 9555064
    Abstract: The invention relates to a Bacillus anthracis (B. anthracis) in which more than one secreted protease is inactivated by genetic modification. Such a protease-deficient B. anthracis has an improved ability to produce recombinant secreted proteins compared to other bacteria, particularly other Bacillus. Improvements include production of intact (i.e., mature full-length) proteins, often at high yield. The disclosure provides a B. anthracis that comprises a genetic modification that inactivates a protease of the M4 family of metalloproteases and a genetic modification that inactivates a protease of the M6 family of metalloproteases. Also provided is a modified B. anthracis comprising such genetic modification transformed with a recombinant molecule encoding a product, as well as methods to prepare and use such B. anthracis.
    Type: Grant
    Filed: August 2, 2012
    Date of Patent: January 31, 2017
    Assignee: The United States of America, as represented by the Secretary Dept. of Health and Human Services
    Inventors: Andrei P. Pomerantsev, Stephen H. Leppla
  • Granzyme B protease variants
    Patent number: 9528101
    Abstract: The present disclosure provides novel variants of enzymes exhibiting serine protease activity; nucleic acid molecules encoding said proteases, vectors, host cells containing the nucleic acids and methods for preparation and producing such enzymes; compositions and complexes comprising at least one of the proteases; and methods for using such enzymes as a part of an immunoprotease, in particular for the treatment of cancer.
    Type: Grant
    Filed: September 21, 2012
    Date of Patent: December 27, 2016
    Assignee: PHARMED ARTIS GmbH
    Inventors: Stefan Barth, Sonja Schiffer, Grit Hehmann-Titt
  • Mutants of staphylokinase carrying amino and carboxy-terminal extensions for polyethylene glycol conjugation
    Patent number: 9518255
    Abstract: The present invention relates to the development of new derivatives of a bacterial plasminogen activator, Staphylokinase (SAK), having one or more amino acid residues with single or multiple cysteines at the amino and/or carboxy terminal ends and their conjugation with PEG (Polyethylene Glycol), resulting in new Staphylokinase derivatives that display altered oligomeric states, enhanced thermal and protease stability and extended plasma half-life. Also included is the cloning and expression in a suitable bacterial host; purification of Staphylokinase derivatives to homogeneity and their chemical modification by integrating a PEG molecule to create new biologically active Staphylokinases having higher protein stability and improved in vivo plasma half life, that may enhance the clinical potential of Staphylokinase in thrombolytic therapy for the treatment of cardiovascular diseases.
    Type: Grant
    Filed: March 12, 2014
    Date of Patent: December 13, 2016
    Assignee: COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH
    Inventors: Satish Singh, Kanak Lata Dikshit
  • Enzymes for inhibiting growth of biofilms and degrading same
    Patent number: 9480729
    Abstract: Enzymes for inhibiting growth of biofilms and degrading biofilms. The enzymes comprise glycosyl hydrolases capable of degrading biofilms. The enzymes are formulated in compositions with and without antimicrobial agents. The enzymes with and without the antimicrobial agents are delivered to biofilms to degrade the biofilms and treat infections of microorganisms associated with the biofilms, delivered to surfaces to inhibit growth of biofilms thereon, and administered to animals to inhibit growth of biofilms therein.
    Type: Grant
    Filed: November 11, 2013
    Date of Patent: November 1, 2016
    Assignee: C5-6 Technologies, Inc.
    Inventor: Phillip J. Brumm
  • Biosensor for detecting RAF/KSR family kinase dimerization and uses thereof
    Patent number: 9464313
    Abstract: Disclosed herein are biosensors useful for detecting the dimerization of RAF and/or KSR polypeptides. These biosensors comprise fusion proteins comprising RAF and/or KSR proteins fused to bioluminescent or fluorescent proteins. Also disclosed are methods of using the biosensors to detect and measure the dimerization of RAF/RAF and RAF/KSR polypeptides by resonance energy transfer such as BRET or FRET, for example to screen for inhibitors of dimerization.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: October 11, 2016
    Assignees: MOUNT SINAI HOSPITAL, UNIVERSITÉ DE MONTRÉAL
    Inventors: Hugo Lavoie, Malha Sahmi, Marc Therrien, Thanashan Rajakulendran, Frank Sicheri
  • Phosphatidic acid phosphatase gene
    Patent number: 9447393
    Abstract: The present invention provides a novel phosphatidic acid phosphatase gene. The object of the present invention can be solved by providing a nucleic acid comprising the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 4, or SEQ ID NO: 5; a protein comprising the amino acid sequence set forth in SEQ ID NO: 2; and mutants thereof.
    Type: Grant
    Filed: July 27, 2012
    Date of Patent: September 20, 2016
    Assignee: SUNTORY HOLDINGS LIMITED
    Inventor: Misa Ochiai
  • Methods and compositions for digestion of organic waste
    Patent number: 9388088
    Abstract: The present invention relates to a process wherein organic material derived from plant and animal material is processed to recover nutritional elements. In particular, there is provided a process for releasing nutritional elements from plant and animal material comprising the steps of treating the material with one or more enzymes to digest said material under appropriate conditions and separating the resulting liquid hydrolysate from the undigested material.
    Type: Grant
    Filed: July 21, 2015
    Date of Patent: July 12, 2016
    Assignee: REDF-ORGANIC RECOVERY, LLC
    Inventors: Jeffrey W. Young, Lewis A. Spencer
  • Performance-enhanced protease variant
    Patent number: 9365844
    Abstract: Proteases encompassing an amino acid sequence that is at least 70% identical to the amino acid sequence indicated in SEQ ID NO. 1 over its entire length, and exhibit the amino acid substitution I21V in the count in accordance with SEQ ID NO. 1, agents that encompass such proteases, display very good cleaning performance on protease-sensitive stains.
    Type: Grant
    Filed: March 10, 2011
    Date of Patent: June 14, 2016
    Assignee: Henkel AG & Co. KGaA
    Inventors: Petra Siegert, Ulrich Schwaneberg, Ronny Martinez, Marion Merkel, Astrid Spitz, Susanne Wieland, Hendrik Hellmuth, Karl-Heinz Maurer
  • Alpha-amylase variants
    Patent number: 9334466
    Abstract: Variants of Bacillus sp. no. 707 alpha-amylase are provided that are produced more efficiently and thus more economically. Higher fermentation yields are achieved through introducing amino acid variations that promote solubility of the variant in a fermentation broth. Increased solubility allows more enzyme to remain in solution after expression in a host cell. This in turn increases the efficiency with which the expressed variant enzyme can be recovered from the fermentation broth.
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: May 10, 2016
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Neelam S. Amin
  • Luciferase derived from lucidina accensa
    Patent number: 9328334
    Abstract: An object of the invention is to provide a novel and useful luciferase. The luciferase according to the embodiments of the invention is derived from Lucidina accensa.
    Type: Grant
    Filed: September 11, 2013
    Date of Patent: May 3, 2016
    Assignee: OLYMPUS CORPORATION
    Inventors: Katsunori Ogo, Ryutaro Akiyoshi, Mariko Murai, Takashi Kinebuchi, Hirobumi Suzuki