Patents Examined by Sheridan Swope
  • Mps1 and KNL1 phosphorylation system
    Patent number: 10669320
    Abstract: Provided herein are compositions and methods for the treatment of cancer by activating the spindle assembly checkpoint (SAC) in cells. In particular, dimerized Mps1 and Spc105/KNL1 constructs are provided as tunable activators of SAC, allowing for control of chromosome segregation accuracy and prevention of aneuploidies that are common in cancer.
    Type: Grant
    Filed: November 18, 2016
    Date of Patent: June 2, 2020
    Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Ajit P. Joglekar, Pavithra Aravamudhan
  • Method for removing viruses in a physiologically-active protein-containing sample
    Patent number: 10654888
    Abstract: A method for manufacturing an antibody formulation in which DNA contaminants are removed by binding the antibody to a protein-A or probtin-G affinity column and eluting the antibody with an acidic eluting solution, preferably of low conductivity.
    Type: Grant
    Filed: July 11, 2014
    Date of Patent: May 19, 2020
    Assignee: CHUGAI SEIYAKU KABUSHIKI KAISHA
    Inventors: Kozo Takeda, Norimichi Ochi, Kimie Ishii, Manabu Matsuhashi, Akinori Imamura
  • Diagnostic and prognostic assays based on circulating tyrosine kinase activity
    Patent number: 10655161
    Abstract: Provided herein are methods for the diagnosis, prognosis, or management of diseases, such as cancer, by measuring the tyrosine kinase activity in acellular body fluids. Further provided are methods of predicting response to therapy in certain populations of cancer patients by contacting an acellular body fluid sample from a patient with a test agent, such as a tyrosine kinase inhibitor, and then measuring the effect of the test agent on tyrosine kinase activity in the sample.
    Type: Grant
    Filed: December 3, 2014
    Date of Patent: May 19, 2020
    Assignee: Quest Diagnostics Investments Incorporated
    Inventor: Chen-Hsiung Yeh
  • Method of treating glycogen storage disease type IX using acid alpha-glucosidase
    Patent number: 10647969
    Abstract: The disclosure relates, in general, to Glycogen Storage Disease and, in particular, to a method of treating Glycogen Storage Disease Type IX and to compounds and compositions suitable for use in such a method, including acid alpha-glucosidase.
    Type: Grant
    Filed: July 25, 2017
    Date of Patent: May 12, 2020
    Assignee: Duke University
    Inventors: Yuan-Tsong Chen, Priya Kishnani, Baodong Sun
  • Modified strains for the production of recombinant silk
    Patent number: 10647975
    Abstract: Disclosed herein are modified strains for reducing degradation of recombinantly expressed products secreted from a host organism and methods of using the modified strains. In some embodiments, to attenuate a protease activity in Pichia pastoris, the genes encoding enzymes the degrade proteases are inactivated or mutated to reduce or eliminate activity. In preferred strains, the protease activity of proteases encoded by PAS_chr4_0584 (YPS1-1) and PAS_chr3_1157 (YPS1-2) (e.g., polypeptides comprising SEQ ID NO: 66 and 67) is attenuated.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: May 12, 2020
    Assignee: Bolt Threads, Inc.
    Inventors: Matthew Scott Gamboa, Joshua Tyler Kittleson
  • Microorganisms for producing putrescine or ornithine and process for producing putrescine or ornithine using them
    Patent number: 10640753
    Abstract: Disclosed is a modified microorganism producing putrescine or ornithine, and a method for producing putrescine or ornithine using the same.
    Type: Grant
    Filed: July 19, 2016
    Date of Patent: May 5, 2020
    Assignee: CJ Cheiljedang Corporation
    Inventors: Su Jin Park, Young Lyeol Yang, Hye Won Um, Hong Xian Li, Kyoung Min Lee, Baek Seok Lee, Hyo Hyoung Lee, Hee Kyoung Jung
  • Subtilase variants and polynucleotides encoding same
    Patent number: 10626388
    Abstract: The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.
    Type: Grant
    Filed: July 6, 2015
    Date of Patent: April 21, 2020
    Assignee: NOVOZYMES A/S
    Inventors: Frank Winther Rasmussen, Peter Kamp Hansen, Lars Lehman Hylling Christensen
  • Amylase variants having reduced disposition to form deamidation products
    Patent number: 10590404
    Abstract: The present invention pertains to the field of amylases with reduced disposition to form deamidation products, particularly alpha-amylases, and compositions comprising such amylases. Also described are means and methods for producing such amylases, and uses of amylases and amylase-comprising compositions, for example for cleaning starch-containing stains, textile desizing, starch liquefaction and saccharification, baking and brewing. The invention also discloses means for increasing amylase purity during fermentation, formulation and storage of amylase or amylase-containing formulations.
    Type: Grant
    Filed: April 15, 2015
    Date of Patent: March 17, 2020
    Assignee: BASF SE
    Inventors: Stefan Jenewein, Stefan Niedermaier
  • Cell-based methods for measuring activity of a protein inhibitor
    Patent number: 10584369
    Abstract: The invention provides a method for determining the activity of an inhibitor of a target protein that has a deleterious effect on cell growth. The principle of the claimed method is that a compound that inhibits the function of an overexpressed protein will relieve the detrimental effect of such overexpression in a concentration-dependent manner, thereby allowing the determination of the activity of the compound in inhibiting its target protein in live cells.
    Type: Grant
    Filed: January 13, 2014
    Date of Patent: March 10, 2020
    Assignee: Senex Biotechnology, Inc.
    Inventors: Igor B Roninson, Donald C Porter, Serena Altilia
  • Rapid bed-side measurement of neutrophil elastase activity in biological fluids
    Patent number: 10577639
    Abstract: The subject invention provides novel devices and methods for the detection and quantification of neutrophil elastase activity in biological samples.
    Type: Grant
    Filed: June 28, 2010
    Date of Patent: March 3, 2020
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: Gregory S. Schultz, Daniel J. Gibson
  • Polypeptides having protease activity
    Patent number: 10563188
    Abstract: The present invention relates to isolated polypeptides having protease activity and isolated nucleic acid sequences encoding the proteases. The invention also relates to nucleic acid constructs, vectors, and host cells, including plant and animal cells, comprising the nucleic acid sequences, as well as methods for producing and using the proteases, in particular the use of the proteases in animal feed.
    Type: Grant
    Filed: April 17, 2018
    Date of Patent: February 18, 2020
    Assignee: Novozymes A/S
    Inventors: Mary Ann Stringer, Tine Hoff, Peter Rahbek Oestergaard, Katrine Fruergaard Pontoppidan
  • Variant proteases and amylases having enhanced storage stability
    Patent number: 10550381
    Abstract: The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.
    Type: Grant
    Filed: July 6, 2015
    Date of Patent: February 4, 2020
    Assignee: NOVOZYMES A/S
    Inventors: Frank Winther Rasmussen, Peter Kamp Hansen, Lars Lehman Hylling Christensen
  • Suppression of prostate cancer using a targeted clostridial neurotoxin
    Patent number: 10550377
    Abstract: The present invention relates to a method for suppressing or treating cancer, in particular to a method for suppressing or treating one or more of colorectal cancer, breast cancer, prostate cancer and/or lung cancer. The therapy employs use of a non-cytotoxic protease, which is targeted to a growth hormone-secreting cell such as to a pituitary cell. When so delivered, the protease is internalised and inhibits secretion/transmission of growth hormone from said cell. The present invention also relates to polypeptides and nucleic acids for use in said methods.
    Type: Grant
    Filed: April 11, 2014
    Date of Patent: February 4, 2020
    Assignee: IPSEN BIOINNOVATION LIMITED
    Inventors: Frederic Madec, Philip Lecane, Philip Marks, Keith Foster
  • Method for prophylaxis or treatment of erbb1 positive cancers using a variant peptidase d with reduced activity
    Patent number: 10478477
    Abstract: Provided are compositions and methods for prophylaxis and/or therapy of ErbB1-positive cancer. The compositions include pharmaceutical preparations that contain isolated or recombinant or modified peptidase D (PEPD) proteins. The methods include prophylaxis and/or therapy of ErbB1-positive cancer by administering a PEPD to an individual who has or is at risk for developing ErbB1-positive cancer.
    Type: Grant
    Filed: August 20, 2015
    Date of Patent: November 19, 2019
    Assignee: Health Research, Inc.
    Inventors: Yuesheng Zhang, Lu Yang, Yun Li, Arup Bhattacharya
  • Acetyl transferases and their use for producing carotenoids
    Patent number: 10465174
    Abstract: The present invention relates to novel acetyl transferases, nucleic acid sequences coding therefore, expression constructs and vectors comprising these sequences, microorganisms transformed therewith and use thereof.
    Type: Grant
    Filed: August 28, 2013
    Date of Patent: November 5, 2019
    Assignee: DSM IP ASSETS B.V.
    Inventors: Christopher Farrell, Peter Houston, Lisa Laprade, Nathalie Balch, Maria Mayorga
  • Engineered proteins with a protease cleavage site
    Patent number: 10421953
    Abstract: Various aspects and embodiments herein relate to recombinant proteins with at least one protease recognition sequence, wherein the recombinant proteins can be inactivated by a cognate protease and methods of preparing such proteins. In some embodiments, recombinant phosphoglucose isomerase (Pgi) proteins are provided. In other embodiments, recombinant phosphotransacetylase (Pta) proteins are provided. In yet other embodiments, recombinant transketolase A (TktA) proteins are provided.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: September 24, 2019
    Assignee: GreenLight Biosciences, Inc.
    Inventors: William Jeremy Blake, Drew S. Cunningham
  • Paint having cell wall particulate material with a protective organophosphorus esterase
    Patent number: 10413769
    Abstract: Disclosed herein are novel coatings and paints comprising a biomolecule composition, wherein the biomolecule composition comprises a phosphoric triester hydrolase. Also disclosed herein are methods of detoxification of a surface contaminated with an organophosphorus compound by contacting the surface with such a coating or paint. Also disclosed herein are novel coating and paint components derived from microorganisms.
    Type: Grant
    Filed: July 9, 2015
    Date of Patent: September 17, 2019
    Assignee: Reactive Surfaces, Ltd., LLP
    Inventor: C. Steven McDaniel
  • Detergent compositions comprising a protease
    Patent number: 10407650
    Abstract: A detergent composition comprising a polypeptide having protease activity is disclosed. The composition may be used in laundry or hard surface cleaning such as automated dish wash, and it has improved performance compared with a commercial protease.
    Type: Grant
    Filed: May 1, 2013
    Date of Patent: September 10, 2019
    Assignee: NOVOZYMES A/S
    Inventors: Benie Astrid, Jeppe Wegener Tams, Peter Rahbek Oestergaard, Morten Gjermansen, Martin Simon Borchert
  • Methods, compositions and kits for high throughput kinase activity screening using mass spectrometry and stable isotopes
    Patent number: 10407712
    Abstract: A mass-spectrometry-based method and substrates are provided herein for large scale kinome activity profiling directly from crude lysates using 90 chemically synthesized peptide substrates with amino acid sequences derived from known phosphoproteins. Quantification of peptide phosphorylation rates was achieved via the use of stable isotope labeled synthetic peptides. A method and substrates for obtaining 90 simultaneous activity measurements in a single-reaction format were developed and validated. The kinome activity profiling strategy was successfully applied with lysates of: cells manipulated by combination of mitogen stimulation, pharmacological perturbation and siRNA-directed kinase knockdown; seven different breast cancer cell lines treated with gefitinib; and each of normal and cancerous tissue samples from renal cell carcinoma patients.
    Type: Grant
    Filed: October 16, 2017
    Date of Patent: September 10, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Steven P. Gygi, Kazuishi Kubota, Judit Villen, Yonghao Yu
  • Methods of producing a protease
    Patent number: 10407674
    Abstract: The present invention relates to methods of producing polypeptides having protease activity.
    Type: Grant
    Filed: May 8, 2018
    Date of Patent: September 10, 2019
    Assignee: NOVOZYMES A/S
    Inventors: Martin Simon Borchert, Jeppe Wegener Tams