Patents by Inventor Alireza Rezania

Alireza Rezania has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • USE OF SMALL MOLECULES TO ENHANCE MAFA EXPRESSION IN PANCREATIC ENDOCRINE CELLS
    Publication number: 20180291348
    Abstract: The present invention provides methods, cell cultures and differentiation media to promote differentiation of pluripotent stem cells to pancreatic endocrine cells of a mature phenotype. The resulting pancreatic endocrine cells express single hormonal insulin, PDX1, NKX6.1, and MAFA. In one or more differentiation stages, culturing may be carried out in a culture vessel at the air-liquid interface.
    Type: Application
    Filed: June 13, 2018
    Publication date: October 11, 2018
    Applicant: Janssen Biotech, Inc.
    Inventor: Alireza Rezania
  • METHODS FOR PURIFYING CELLS DERIVED FROM PLURIPOTENT STEM CELLS
    Publication number: 20180258401
    Abstract: The present invention is directed to methods to differentiate pluripotent stem cells. In particular, the present invention provides methods of characterization of cells differentiated into cells expressing markers characteristic of the pancreatic endocrine lineage utilizing unique surface markers. The present invention also provides methods to enrich or sort cells expressing markers characteristic of the pancreatic endocrine lineage. The present invention also provides methods to deplete cells that may contaminate populations of cells expressing markers characteristic of the pancreatic endocrine lineage formed by the methods of the present invention, thereby reducing the incidence of tumor formation in vivo following transplantation.
    Type: Application
    Filed: May 14, 2018
    Publication date: September 13, 2018
    Applicant: JANSSEN BIOTECH, INC.
    Inventors: Francis Karanu, Alireza Rezania
  • DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS
    Publication number: 20180258402
    Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells and the products related to or resulting from such methods. In particular, the present invention provides an improved method for the formation of pancreatic hormone expressing cells and pancreatic hormone secreting cells. In addition, the present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer and the products related to or resulting from such methods. The present invention also provides methods to promote glucose-stimulated insulin secretion in insulin-producing cells derived from pluripotent stem cells.
    Type: Application
    Filed: May 14, 2018
    Publication date: September 13, 2018
    Applicant: LIFESCAN INC.
    Inventor: Alireza Rezania
  • Differentiation of human embryonic stem cells into pancreatic endocrine cells
    Patent number: 10066210
    Abstract: The present invention provides methods to promote differentiation of pancreatic endoderm cells to pancreatic endocrine rich clusters and to enhance insulin expression in hormone-expressing cells.
    Type: Grant
    Filed: June 6, 2013
    Date of Patent: September 4, 2018
    Assignee: Janssen Biotech, Inc.
    Inventor: Alireza Rezania
  • Use of small molecules to enhance MAFA expression in pancreatic endocrine cells
    Patent number: 10006006
    Abstract: The present invention provides methods, cell cultures and differentiation media to promote differentiation of pluripotent stem cells to pancreatic endocrine cells of a mature phenotype. The resulting pancreatic endocrine cells express single hormonal insulin, PDX1, NKX6.1, and MAFA. In one or more differentiation stages, culturing may be carried out in a culture vessel at the air-liquid interface.
    Type: Grant
    Filed: May 7, 2015
    Date of Patent: June 26, 2018
    Assignee: Janssen Biotech, Inc.
    Inventor: Alireza Rezania
  • Differentiation of human embryonic stem cells
    Patent number: 9969982
    Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells and the products related to or resulting from such methods. In particular, the present invention provides an improved method for the formation of pancreatic hormone expressing cells and pancreatic hormone secreting cells. In addition, the present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer and the products related to or resulting from such methods. The present invention also provides methods to promote glucose-stimulated insulin secretion in insulin-producing cells derived from pluripotent stem cells.
    Type: Grant
    Filed: May 21, 2015
    Date of Patent: May 15, 2018
    Assignee: LifeScan, Inc.
    Inventor: Alireza Rezania
  • Methods for purifying cells derived from pluripotent stem cells
    Patent number: 9969981
    Abstract: The present invention is directed to methods to differentiate pluripotent stem cells. In particular, the present invention provides methods of characterization of cells differentiated into cells expressing markers characteristic of the pancreatic endocrine lineage utilizing unique surface markers. The present invention also provides methods to enrich or sort cells expressing markers characteristic of the pancreatic endocrine lineage. The present invention also provides methods to deplete cells that may contaminate populations of cells expressing markers characteristic of the pancreatic endocrine lineage formed by the methods of the present invention, thereby reducing the incidence of tumor formation in vivo following transplantation.
    Type: Grant
    Filed: February 28, 2011
    Date of Patent: May 15, 2018
    Assignee: Janssen Biotech, Inc.
    Inventors: Francis Karanu, Alireza Rezania
  • GENERATION OF HUMAN PLURIPOTENT STEM CELL DERIVED FUNCTIONAL BETA CELLS SHOWING A GLUCOSE-DEPENDENT MITOCHONDRIAL RESPIRATION AND TWO-PHASE INSULIN SECRETION RESPONSE
    Publication number: 20170362572
    Abstract: The invention provides for methods of differentiating pancreatic endocrine cells into pancreatic beta cells expressing PDX1, NKX6.1, MAFA, UCN3 and SLC2A. These pancreatic beta cells may be obtained by step-wise differentiation of pluripotent stem cells. The pancreatic beta cells exhibit glucose-dependent mitochondrial respiration and glucose-stimulated insulin secretion similar to islet cells.
    Type: Application
    Filed: June 14, 2017
    Publication date: December 21, 2017
    Applicant: Janssen Biotech, Inc.
    Inventors: Sebastian Rieck, Alireza Rezania
  • DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS
    Publication number: 20170355963
    Abstract: The present invention provides a method for increasing the expression of MAFA in cells expressing markers characteristic of the pancreatic endocrine lineage comprising the steps of culturing the cells expressing markers characteristic of the pancreatic endocrine lineage in medium comprising a sufficient amount of a cyclin-dependent kinase inhibitor to cause an increase in expression of MAFA.
    Type: Application
    Filed: August 22, 2017
    Publication date: December 14, 2017
    Applicant: Janssen Biotech, Inc.
    Inventors: Alireza Rezania, Benjamin Fryer
  • Differentiation of Human Embryonic Stem Cells
    Publication number: 20170335289
    Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides an improved method for the formation of pancreatic endoderm, pancreatic hormone expressing cells and pancreatic hormone secreting cells. The present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer.
    Type: Application
    Filed: July 26, 2017
    Publication date: November 23, 2017
    Applicant: LifeScan, Inc.
    Inventors: Alireza Rezania, Jean Xu
  • DIFFERENTIATION OF PLURIPOTENT STEM CELLS TO INTESTINAL MIDGUT ENDODERM CELLS
    Publication number: 20170296594
    Abstract: Cell populations of intestinal midgut endoderm cells and methods of generating the cells expressing markers characteristic of intestinal endoderm lineage are disclosed. Methods of treating conditions such as diabetes are also disclosed.
    Type: Application
    Filed: April 4, 2017
    Publication date: October 19, 2017
    Applicant: Janssen Biotech, Inc.
    Inventors: Sebastian Rieck, Alireza Rezania
  • Differentiation of human pluripotent stem cells
    Patent number: 9752126
    Abstract: The present invention provides a method for increasing the expression of MAFA in cells expressing markers characteristic of the pancreatic endocrine lineage comprising the steps of culturing the cells expressing markers characteristic of the pancreatic endocrine lineage in medium comprising a sufficient amount of a cyclin-dependent kinase inhibitor to cause an increase in expression of MAFA.
    Type: Grant
    Filed: December 9, 2015
    Date of Patent: September 5, 2017
    Assignee: Janssen Biotech, Inc.
    Inventors: Alireza Rezania, Benjamin Fryer
  • Differentiation of human embryonic stem cells
    Patent number: 9725699
    Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides an improved method for the formation of pancreatic endoderm, pancreatic hormone expressing cells and pancreatic hormone secreting cells. The present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer.
    Type: Grant
    Filed: March 18, 2014
    Date of Patent: August 8, 2017
    Assignee: LifeScan, Inc.
    Inventors: Alireza Rezania, Jean Xu
  • DEFINED MEDIA FOR EXPANSION AND MAINTENANCE OF PLURIPOTENT STEM CELLS
    Publication number: 20170183626
    Abstract: The present invention provides methods to promote the proliferation of undifferentiated pluripotent stem cells in defined media. Specifically, the invention provides a defined cell culture formulation for the culture, maintenance, and expansion of pluripotent stem cells, wherein culturing stem cells in the defined cell culture formulation maintains the pluripotency and karyotypic stability of the cells for at least 10 passages. Further disclosed is a cell population, grown under defined media conditions, that expresses OCT4, SOX2, NANOG, and FOXA2.
    Type: Application
    Filed: March 13, 2017
    Publication date: June 29, 2017
    Applicant: Janssen Biotech, Inc.
    Inventor: Alireza Rezania
  • DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS
    Publication number: 20170081634
    Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells, wherein greater than 85% of the cells in the population express markers characteristic of the definitive endoderm lineage.
    Type: Application
    Filed: December 2, 2016
    Publication date: March 23, 2017
    Applicant: Janssen Biotech, Inc.
    Inventor: Alireza Rezania
  • Defined media for expansion and maintenance of pluripotent stem cells
    Patent number: 9593307
    Abstract: The present invention provides methods to promote the proliferation of undifferentiated pluripotent stem cells in defined media. Specifically, the invention provides a defined cell culture formulation for the culture, maintenance, and expansion of pluripotent stem cells, wherein culturing stem cells in the defined cell culture formulation maintains the pluripotency and karyotypic stability of the cells for at least 10 passages. Further disclosed is a cell population, grown under defined media conditions, that expresses OCT4, SOX2, NANOG, and FOXA2.
    Type: Grant
    Filed: May 9, 2016
    Date of Patent: March 14, 2017
    Assignee: Janssen Biotech, Inc.
    Inventor: Alireza Rezania
  • DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS INTO PANCREATIC ENDOCRINE CELLS
    Publication number: 20170044499
    Abstract: The present invention provides methods to promote differentiation of pancreatic endoderm cells to pancreatic endocrine rich clusters and to enhance insulin expression in hormone-expressing cells.
    Type: Application
    Filed: November 1, 2016
    Publication date: February 16, 2017
    Applicant: Janssen Biotech, Inc.
    Inventor: Alireza Rezania
  • SC-BETA CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME
    Publication number: 20170029778
    Abstract: Disclosed herein are methods, compositions, kits, and agents useful for inducing ? cell maturation, and isolated populations of SC-? cells for use in various applications, such as cell therapy.
    Type: Application
    Filed: June 11, 2014
    Publication date: February 2, 2017
    Inventors: Quinn P. Peterson, Felicia J. Pagliuca, Douglas A. Melton, Jeffrey R. Millman, Michael Saris Segel, Mads Gürtler, Alireza Rezania, Benjamin Fryer
  • Differentiation of human embryonic stem cells
    Patent number: 9528090
    Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells, wherein greater than 85% of the cells in the population express markers characteristic of the definitive endoderm lineage.
    Type: Grant
    Filed: August 17, 2011
    Date of Patent: December 27, 2016
    Assignee: Janssen Biotech, Inc.
    Inventor: Alireza Rezania
  • Differentiation of pluripotent stem cells
    Patent number: 9458430
    Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method utilizing a CYP26A inhibitor to produce a population of pancreatic endocrine precursor cells.
    Type: Grant
    Filed: October 14, 2015
    Date of Patent: October 4, 2016
    Assignee: Janssen Biotech, Inc.
    Inventor: Alireza Rezania