Abstract: The present invention provides reagents, kits, and methods for single-cell whole genome amplification using Phi 29 DNA polymerase.

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

Abstract: Polymer-based drilling fluids may be used as an alternative to drilling fluids containing bentonite or other clay materials, although filter cake formation with polymer-based drilling fluids can often be inadequate. Drilling methods can comprise: providing a drilling fluid comprising a carrier fluid, a non-crosslinked hydroxyalkylcellulose polymer, and a plurality of non-biodegradable particulates; drilling a borehole in the presence of the drilling fluid; and forming a filter cake within the borehole, the filter cake comprising the non-crosslinked hydroxyalkylcellulose polymer and the non-biodegradable particulates.

Abstract: The present invention provides reagents, kits, and methods for single-cell whole genome amplification using Phi 29 DNA polymerase.

Abstract: The permeability of barrier layers formed from sodium bentonite clay may be significantly and unpredictably impacted upon contacting various ionic substances. Modifying agents may be admixed with sodium bentonite clay to lessen the impacts resulting from its contact with an ionic substance. Suitable modifying agents may comprise an anionic substance selected from the group consisting of a diutan, a polymer containing a 2-acrylamido-2-methylpropanesulfonic acid monomer, any salt thereof, and any combination thereof. Methods for forming a barrier layer may comprise: providing a barrier material comprising sodium bentonite clay and a modifying agent admixed with the sodium bentonite clay, the modifying agent comprising an anionic substance selected from the group consisting of a diutan, a polymer containing a 2-acrylamido-2-methylpropanesulfonic acid monomer, any salt thereof, and any combination thereof; and forming a barrier layer comprising the barrier material.

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing.

Abstract: A wellbore treatment fluid comprising: a base fluid; and a fluid loss additive package, the fluid loss additive package comprising: a water-swellable, superabsorbent polymer; a derivatized cellulose; and non-swellable particles that are insoluble in the base fluid. A method of drilling a wellbore into a subterranean formation comprising: providing the drilling fluid; and forming the wellbore using a drill bit and the drilling fluid.

Abstract: A device configured to couple to a wheel assembly of a mobile device is provided. The device includes a first side portion configured to couple to a first lateral side of the wheel assembly. The device also includes a second side portion configured to couple to a second lateral side of the wheel assembly opposite the first lateral side, wherein the first and second side portions are compliant mechanisms configured to both move a movable obstacle out of a path of the mobile device and to retract in response to a force exerted on the first and second side portions by a fixed obstacle disposed along the path.

Abstract: The presence of unmitigated metal ions in a drilling fluid can prevent acceptable rheological and fluid loss control performance from being realized during a drilling operation. Softening of a hard water source containing alkaline earth metal ions may allow the resultant treated water source to be used in formulating a drilling fluid with acceptable properties. Not all softening agents perform equivalently in this regard, and some softening agents may not be readily available. Methods for formulating a drilling fluid may comprise: providing a hard water source comprising one or more metal ions; introducing an alkali metal silicate into the hard water source to form a treated water source; and after forming the treated water source, adding a viscosifying agent to the treated water source to form a drilling fluid. The methods may further comprise drilling a borehole with the drilling fluid.

Abstract: Polymer-based drilling fluids may be used as an alternative to drilling fluids containing bentonite or other clay materials, although filter cake formation can often be inadequate when using polymer-based drilling fluids. As a result, drilling methods employing polymer-based drilling fluids may sometimes experience unacceptable fluid loss performance within a borehole. Drilling methods with enhanced fluid loss performance may comprise: providing a drilling fluid comprising a carrier fluid, a viscosity-enhancing polymer, and a plurality of non-biodegradable particulates, the non-biodegradable particulates comprising at least silica particulates; drilling a borehole in the presence of the drilling fluid; and forming a filter cake within the borehole, the filter cake comprising the non-biodegradable particulates.

Abstract: Methods of circulating a drilling fluid while drilling a shallow wellbore that is highly deviated or horizontal. The drilling fluid comprises an aqueous base fluid, iota carrageenan, partially hydrolyzed polyacrylamide, and a divalent cation selected from the group consisting of a calcium ion, a magnesium ion, and any combination thereof. The shallow wellbore that is highly deviated or horizontal is no greater than about 610 meters below an earth surface, and in a range of about 55° to about 125° from a vertical inclination.

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing.

Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out “in parallel,” i.e., essentially simultaneously in the separate reaction volumes.

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

Abstract: A wellbore treatment fluid comprising: a base fluid; and a fluid loss additive package, the fluid loss additive package comprising: a water-swellable, superabsorbent polymer; a derivatized cellulose; and non-swellable particles that are insoluble in the base fluid. A method of drilling a wellbore into a subterranean formation comprising: providing the drilling fluid; and forming the wellbore using a drill bit and the drilling fluid.

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

Abstract: Polymer-based drilling fluids may be used as an alternative to drilling fluids containing bentonite or other clay materials, although filter cake formation with polymer-based drilling fluids can often be inadequate. Drilling methods can comprise: providing a drilling fluid comprising a carrier fluid, a non-crosslinked hydroxyalkylcellulose polymer, and a plurality of non-biodegradable particulates; drilling a borehole in the presence of the drilling fluid; and forming a filter cake within the borehole, the filter cake comprising the non-crosslinked hydroxyalkylcellulose polymer and the non-biodegradable particulates.

Abstract: Dry drilling fluid additives may include a dry mixture that comprises a clay stabilizing agent at about 76% to about 93% by weight of the dry mixture, a dispersant at about 3% to about 6% by weight of the dry mixture, and a surfactant at about 4% to about 18% by weight of the dry mixture. Such dry drilling fluid additives may be useful in methods and systems for drilling wellbore penetrating subterranean formations, especially deviated and highly-deviated wellbores.

Abstract: Dry drilling fluid additives may include a dry mixture that comprises a clay stabilizing agent at about 76% to about 93% by weight of the dry mixture, a dispersant at about 3% to about 6% by weight of the dry mixture, and a surfactant at about 4% to about 18% by weight of the dry mixture. Such dry drilling fluid additives may be useful in methods and systems for drilling wellbore penetrating subterranean formations, especially deviated and highly-deviated wellbores.

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing.