Abstract: Disclosed herein are compositions comprising non-naturally occurring zinc finger domains, fusion proteins comprising these zinc finger domains, polynucleotides encoding these proteins, cells expressing these proteins and pharmaceutical compositions comprising these proteins or polynucleotides as well as methods of modifying an Htt gene using these compositions for treating or preventing Huntington's Disease.

Abstract: Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.

Abstract: A mirror assembly for use with mirror housing on autonomous or semi-autonomous vehicle, including a reflector for collecting and distributing emitted light to a plurality of icon regions, a circuit board, a plurality of light sources mounted to the circuit board and positioned to selectively emit light to illuminate the icon regions, wherein the circuit board provides selective illumination of the icon regions via the light sources in response to one or more received signal inputs from the vehicle indicating an imminent autonomous vehicle maneuver, a mirror having a front surface and a rear surface, wherein the reflector is secured at least indirectly to the rear surface and a plurality of icons visible along a driver viewing axis relative to the front surface of the mirror during illumination, wherein the plurality of icons are positioned over the icon regions to allow for selective icon illumination via a selected icon region.

Abstract: A portable charger capable of jump starting a 12 V car battery includes a charger battery, a jump start circuit operatively electrically connected with the charger battery and with an ignition power outlet, and a microcontroller for coordinating safety functions to establish or interrupt the operative electrical connection of the jump start circuit with the ignition power outlet. The ignition power outlet comprises a positive power socket, a negative power socket, a positive sensing socket and a negative sensing socket. The sensing sockets are electrically isolated from the power sockets, and the microcontroller senses voltage across the sensing sockets and is configured to interrupt the operative electrical connection of the jump start circuit to the ignition power outlet until proper voltage is sensed across the sensing sockets.

Abstract: A reaction controlled system of includes a track, a trolley that travels along the track, an animated figure coupled to the trolley, at least one reaction control element coupled to a portion of the animated figure (e.g., on the features, limbs, and/or body of the animated figure), and a controller. The controller detects presence of a ride vehicle or a guest. The controller also controls the trolley to position the animated figure based on the presence of the ride vehicle or the guest. The controller also controls animation of the animated figure by operating the reaction control element.

Abstract: A modular energy system including a header module configured to removably connect to an energy module. The energy module can comprise a port configured to deliver one or more energy modalities to a surgical instrument connected thereto. The header module can comprise a display screen configured to display a user interface. The header module can further include a control circuit configured to detect attachment of energy modules to the modular energy system and control the display of the user interface to display UI portions for each connected module and reconfigure the displayed UI portions to accommodate the new UI portions as additional energy modules are connected to the modular energy system.

Abstract: A modular energy system that can include a header module removably couplable to one or more energy modules. The one or more energy modules collectively comprise multiple ports to which a surgical instrument is connectable and are each configured to drive a plurality of energy modalities for the surgical instrument. The header module can comprise a display screen configured to display a user interface. The header module is connectable to a footswitch such that the header module can receive a control signal from the footswitch and can send a control signal to the footswitch. The header module can further comprise configured to assign the footswitch to a particular port and, based on user input received via the user interface, reassign the footswitch to another of the ports.

Abstract: A fluid pump system having a first fluid pump for pumping fluid from a source to a surgical site and a second fluid pump for removing fluid from the surgical site at a first predetermined rate, wherein said fluid pump system intermittently operates in conjunction with a surgical tool which, when operational, removes fluid from the surgical site at a second predetermined rate greater than said first predetermined rate, the improvement including a sensor for sensing a predetermined parameter of the surgical tool and providing an output signal indicating that the surgical tool is operating; and an actuating means responsive to said output signal to actuate said second fluid pump to remove fluid from the surgical site at second predetermined rate.

Abstract: Provided herein, inter alia, are engineered antibodies or antigen-binding fragments thereof that exhibit one or more improved properties relating to manufacturability, thermostability, and/or protease resistance as well as methods for making and using the same.

Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, isoprenoids, and/or acetyl-CoA-derived products in recombinant microorganisms by engineering the microorganisms to comprise one or more acetylating proteins such that the expression and/or activity of the one or more acetylating proteins is modulated.

Abstract: An anchor is provided for placement in or against tissue. The anchor includes a flat fibrous construct having a first end and a second end; and a filament having a first end and a second end passing through the fibrous construct in two locations including a first passing location nearest a first end of the fibrous construct, a second passing location nearest a second end of the fibrous construct, the filament remaining free to slide through the first passing location and the second passing location such that the filament can be removed from the flat fibrous construct from the first end of the fibrous construct and the second end of the fibrous construct. The anchor can be inserted into a bone hole and deployed.

Abstract: A bottom hole assembly has a tubular component with an axis, a first axial end, a second axial end and a poppet valve assembly mounted in the second axial end. An orifice retainer is mounted inside the tubular component adjacent the first axial end. The orifice retainer has a retainer first axial end, a retainer second axial end opposite the retainer first axial end and an orifice pocket adjacent the retainer second axial end. The orifice retainer is installed in and can be removed from the first axial end of the tubular component, opposite the poppet valve assembly. In addition, an orifice is mounted in the orifice pocket of the orifice retainer. The orifice operates in conjunction with the poppet valve assembly.

Abstract: Rotary heat exchangers can include a ride-along compressor, at least a portion of which can be rotated along with the heat exchanger. By rotating at least a portion of the compressor along with the heat exchanger, a sealed fluid circuit containing a two-phase working fluid can be provided. A rotary heat pump or heat engine can include an evaporator and a condenser in the form of back-to-back centrifugal fans. The centrifugal fan blades or other portions of the evaporator and condenser may include internal cavities where the working fluid undergoes a phase change.

Abstract: The present disclosure relates to methods, devices, and systems for measuring nitric oxide released from a material. For example, a method of measuring nitric oxide release from a material can include introducing a continuous flow of a carrier gas into a sample holding chamber via a carrier gas inlet at an effective flow rate, introducing an amount of the nitric oxide releasing material into the sample holding chamber via a separate sample inlet to contact the continuous flow of the carrier gas, directing the carrier gas and released nitric oxide out of the sample holding chamber via a nitric oxide outlet toward a nitric oxide detector, and quantifying an amount of released nitric oxide using the nitric oxide detector.

Abstract: An apparatus is described. The apparatus includes a rack shelf back interface. The rack shelf back interface has first and second flanges to mount to a rack’s rear facing rack mounts. The rack shelf back interface has a power connector610 on an inside of a back face of the rack shelf interface, the power connector to mate with a corresponding power connector on an electronic system that is to be installed in the rack shelf. The rack shelf back interface has an alignment feature on the inside of the back face of the rack shelf interface to ensure that the power connector properly mates with the corresponding power connector.

Abstract: A fenestration assembly includes a sash, a frame surrounding the sash, and at least one screen assembly mounted in at least one of: the top portion and the bottom portion. The frame includes a top portion, a bottom portion, and two jambs forming first slots extending lengthwise along at least a portion of the jamb. The first slots each include a screen edge retention feature. The screen assembly includes a roller assembly substantially hidden from view, a screen material attached to the roller assembly, and a plurality of raised features. Edges of the screen material extend into the first slots. The raised features are attached to each of the edges of the screen material. The raised features are configured to engage the screen edge retention features and retain each of the edges of the screen material at least partially within the first slot of each of the two jambs.

Abstract: Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.

Abstract: A grasping tool (10) has a pair of elongate arms (20) attached via a hinge element (26). The elongate arms (20) provide gripping surfaces (28) for grasping an item therebetween. A finger engagement element (30) extends from each of the elongate arms, (20) spaced a distance from the proximal end (22). Each elongate arm (20) is generally disposed on a first axis, and each finger engagement element (30) is disposed around a second axis, such that the second axis is approximately parallel to and located below the first axis, such that the first and second axes are spaced a distance such that the pair of elongate arms (20) are positioned above the fingers of the user when the user's fingers are positioned through the finger engagement elements (30).

Abstract: Provided herein, inter alia, are fusion DNA constructs comprising improved protease recognition sequences for expressing and purifying one or more polypeptides of interest as well as methods for producing one or more polypeptides of interest in a recombinant host cell.

Abstract: Systems and methods are described for modifying input and output (I/O) to an object storage service by implementing any combination of and any number of owner-specified functions and native functions. A function can implement a data manipulation. The functions can be applied prior to implementing a request method (e.g., GET, PUT, LIST, etc.) specified within the I/O request, such that the data to which the method is applied may not match the object specified within the request. For example, a user may request to obtain a data set. The data set may be passed to a native function that filters sensitive data to the data set, the output of the native function may be passed to an owner-specified function that redacts data from the filtered data set, and the request method may then be applied to the output of the owner-specified function.