Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues of organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provide dare methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.

Abstract: A method of fabricating a display panel includes forming a first conductive layer on a base substrate, wherein the first conductive layer is formed in an encapsulated area and a peripheral area of the display panel; forming an organic insulating layer on a side of the first conductive layer away from the base substrate, wherein the organic insulating layer is formed to be limited in the encapsulated area; forming a first inorganic insulating layer on a side of the organic insulating layer away from the base substrate; forming a second conductive layer on a side of the organic insulating layer and the first inorganic insulating layer away from the base substrate, wherein the second conductive layer is formed in the encapsulated area and the peripheral area; and forming a second inorganic insulating layer, the second inorganic insulating layer formed between the organic insulating layer and the first conductive layer.

Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a novel RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.

Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a novel RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.

Abstract: An intelligent massage chair is provided. The chair includes a chair frame and armrests connected on left and right sides of the chair frame. At least one of the armrests is connected to the chair frame through a flipping device. The flipping device includes a base connected to the chair frame and a flipping fixed seat rotatably connected to the base around a rotation axis extending left and right, the flipping fixed seat is fixed to the at least one of the armrests. When the flipping device flips the armrest to a set position, the chair frame has a side entrance on the side of the armrest. The armrest can be rotated open from the side to form a side entrance for users to sit in the massage chair from the left and right sides, which is convenient for users to sit in the massage chair.

Abstract: The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

Abstract: A data readout circuit of a RRAM includes: an adaptive current sense amplifier (CSA) and a reference current generator, the adaptive CSA is configured to electrically connect to the RRAM, and the adaptive CSA is electrically connected to the reference current generator; the reference current generator is configured to generate a basic reference current; the adaptive CSA is configured to obtain a reference current according to the basic reference current and a bit-line current of the RRAM; and the adaptive CSA is configured to compare the size of the reference current and that of the bit-line current so as to read out stored data. The present disclosure can improve the problem of data readout error due to the degradation of high resistance state of the RRAM.

Abstract: The disclosure provides novel programmable targeting sequences and applications thereof. The targeting sequences can be engineered for binding to proteins, polypeptides, and other macromolecules.

Abstract: An efficient structure and methodology for container-based applications are provided for disaster recovery for data in a storage system using application consistency groups for replication from the storage system to a disaster recovery system. Application consistency groups can be generated containing applications, a disaster recovery plan, and a disaster recovery policy, where each application has a consistency level. The applications in an application consistency group can include stateless applications and stateful applications. The replication of an application consistency group from the system to a disaster recovery storage system can be controlled according to the disaster recovery policy, the disaster recovery plan, and the consistency levels of the application consistency group.

Abstract: Integrated circuit structures having recessed trench contacts and deep boundary vias are described. For example, an integrated circuit structure includes a plurality of gate lines extending over a plurality of semiconductor nanowire stack channel structures. A plurality of trench contacts extends over a plurality of source or drain structures, where a first one of the plurality of trench contacts has a recess therein. A backside metal routing layer is extending beneath the plurality of gate lines and beneath the plurality of trench contacts. A conductive structure couples the backside metal routing layer to a second one of the one or more of the plurality of trench contacts. The conductive structure includes a pillar portion in contact with the second one of the plurality of trench contacts, the pillar portion on a line portion, the line portion in contact with and extending along the backside metal routing layer.

Abstract: Embodiments of the present application disclose a method for manufacturing a naked-eye 3D device and a naked-eye 3D device. The method includes: forming a display module including a plurality of pixel islands; forming a spacer layer on the display module; and forming a micro-lens array on the spacer layer, wherein the spacer layer is formed to have a thickness such that the plurality of pixel islands are located at a focal plane of the micro-lens array. The method further includes: forming an alignment mark between the spacer layer and the display module, wherein the alignment mark is used for, when forming the micro-lens array, aligning each micro-lens in the micro-lens array with one of the plurality of pixel islands.

Abstract: The present disclosure discloses a method and a device for continuously synthesizing cyclopropane compounds. The method includes the following steps: continuously performing a synthetic reaction of a diazomethane precursor in a first reactor, the reaction product of the first reactor flowing into a separator for stratification, the organic phase obtained by stratification overflowing into a second reactor, continuously consuming the diazomethane precursor in a second reactor to prepare diazomethane and performing an electron-rich monoolefin cyclopropanation reaction in situ so as to obtain the cyclopropane compound.

Abstract: The application relates to a deep scanning mutagenesis library to interrogate phenotypic changes in a population of cells comprising a plurality of CRISPR-Cas system guide RNAs targeting genomic sequences within at least one continuous genomic region, wherein the guide RNAs target at least 100 genomic sequences upstream of a PAM sequence for every 1000 base pairs within the continuous genomic region and methods for their use.

Abstract: A heat exchanger including: a collecting pipe that has a collecting pipe wall; and a plurality of flat pipes. The plurality of flat pipes include two groups of first flat pipes, which are positioned on the outermost side in an axial direction of the collecting pipe, and a second flat pipe, which is positioned between the two groups of first flat pipes; and the collecting pipe includes two first parts, which correspond to the two groups of first flat pipes in the axial direction of the collecting pipe, and a second part, which is positioned between the two first parts.

Abstract: Disclosed is a method for recommending real-time audios and/or videos, including acquiring description data of a plurality of real-time audios and/or videos; acquiring user data; determining at least one candidate real-time audio and/or video in the real-time audios and/or videos; acquiring audio and/or video data of the at least one candidate real-time audio and/or video upon initiation of the candidate real-time audio and/or video; acquiring content data of the at least one candidate real-time audio and/or video; acquiring a degree of association between the content data of the at least one candidate real-time audio and/or video and the user data; and displacing a target candidate real-time audio and/or video in the at least one candidate real-time audio and/or video in a user interface.

Abstract: The present disclosure discloses a data readout circuit of a RRAM and a RRAM circuit. The data readout circuit of the RRAM comprises: an adaptive current sense amplifier (CSA) and a reference current generator, the adaptive CSA is configured to electrically connect to the RRAM, and the adaptive CSA is electrically connected to the reference current generator; the reference current generator is configured to generate a basic reference current; the adaptive CSA is configured to obtain a reference current according to the basic reference current and a bit-line current of the RRAM; and the adaptive CSA is configured to compare the size of the reference current and that of the bit-line current so as to read out stored data. The present disclosure can improve the problem of data readout error due to the degradation of high resistance state of the RRAM.

Abstract: A vacuum switching valve and a suction system having the same. The vacuum switching valve comprises: a valve body, comprising a first end and a second end, the second end being provided with an air inlet, an air outlet and a through hole; a valve element movably arranged in the valve body; a cylinder, the cylinder being connected to the first end and the valve element, the cylinder drives the valve element to move in the valve body, to close or open the air inlet; a stopper passing through the through hole, the stopper comprising a third end and a fourth end, the third end being connected to the valve element, the fourth end being located on the side of the through hole away from the valve element.

Abstract: This application relates to a galvanometer-based laser synchronization controlling method, calibration method and apparatus, and a LiDAR. The galvanometer-based laser synchronization controlling method includes obtaining a fast-axis feedback signal when a galvanometer scans; obtaining a first phase difference between a fast-axis drive signal and the fast-axis feedback signal and obtaining a second phase difference between an emission period of a laser beam and the fast-axis drive signal; and setting a phase for the fast-axis drive signal based on the first phase difference and the second phase difference.

Abstract: The embodiments of this application disclose a time of flight measurement method, including: performing delay processing on an echo signal to obtain N delayed signals; generating X clock signals with different phases based on a multi-phase clock unit; performing delay latch on the N delayed signals based on each of the X clock signals; determining a to-be-processed time of flight based on the reference signal and each of the delay latch result; and determining a target time of flight based on the X to-be-processed time of flights, where the target time of flight is a time difference between emitting the reference signal and receiving the echo signal. With the embodiment of this application, the precision of measuring the time of flight of the echo signal can be effectively improved while saving costs and simplifying calculations.