Abstract: Disclosed is a method for correlating at least one amino acid sequence from an antibody isolated from human or animal blood with at least one DNA sequence corresponding to the antibody in the immunorepertoire of the human or animal. The method also provides a means for pairing heavy and light chains to produce synthesized monoclonal antibodies.

Abstract: Disclosed is a method for amplifying RNA and/or DNA from immune cell populations and using the amplified products to produce an immune response profile and evaluate the possible correlation between a normal or abnormal immune response and the development of a disease such as an autoimmune disease, cancer, diabetes, or heart disease.

Abstract: The present disclosure generally pertains to a multiplex method for analyzing samples comprising using polynucleotide amplification to produce amplified products wherein one or more target sequences are tagged with a non-interfering, non-canceling target-specific polynucleotide identification tag, pyrosequencing the amplified products through the non-canceling target-specific polynucleotide identification tag sequence to detect the presence of one or more specific polynucleotide identification tags. The presence of a specific polynucleotide identification tag being correlated with the presence of a specific target sequence.

Abstract: The present invention discloses a system and method for CSCF entity disaster tolerance and load balancing the system comprises P-CSCF entities, I-CSCF entities and S-CSCF entities, and further comprises a DNS Server. The present invention uses a DNS UPDATE message to report the load equivalent weight of the CSCF entity at regular time, so that the DNS Server can use the load equivalent weight when executing the load balancing strategy. It makes the disaster tolerance and load balancing in the IMS network be much easier to use and extend, thus reducing the load of the IMS network.

Abstract: Embodiments of the present disclosure generally pertain to systems and methods for performing amplicon rescue multiplex polymerase chain reaction (arm-PCR). In one embodiment, the system comprises a processor and a reader coupled to a control element. The control element is configured to control the operation of the processor and the reader based on a variety of settings. The processor is configured to receive a self-contained cassette for performing PCR amplification of DNA and/or RNA obtained from an organic specimen. The processor engages with the cassette and manipulates reagents within the cassette in order to amplify and detect the DNA from the specimen. The processor also causes the cassette to deposit the DNA on a microarray within the cassette. The reader is configured to receive the cassette after it has been processed by the processor and to capture an image of the microarray for transmission to the control element as test data.

Abstract: Disclosed is an isolated strain of a previously unknown Staphylococcus, Staphylococcus pseudolugdunensis. Also disclosed are the sequences of the S. pseudolugdunensis tuf gene and 16s rRNA and methods for distinguishing S. pseudolugdunensis from other staphylococcal species.

Abstract: A cassette for performing multiplex PCR is described. The cassette allows for the automated amplification and detection of multiple nucleic acid targets while limiting contamination. The device facilitates a two-amplification, two-primer PCR protocol which results in highly specific, highly sensitive and semi-quantitative amplification of multiple nucleic acid targets. The cassette contains a cavity, a base with a plurality of chambers, a movable pipette for the transfer of reagents and a detection area. The cassettes are single use, disposable devices which may be loaded with reagents needed for the detection of multiple targets. The cassettes may be used in a hospital or clinical setting to quickly screen clinical samples and detect the presence of infectious agents.

Abstract: Disclosed is a method for calculating a Polymerase Preference Index (PPI) for potential primers for DNA sequencing and/or amplification, and thereby increasing the efficiency of DNA sequencing and/or amplification performed using primers selected according to their PPI number.

Abstract: The present invention discloses a system and method for CSCF entity disaster tolerance and load balancing the system comprises P-CSCF entities, I-CSCF entities and S-CSCF entities, and further comprises a DNS Server. The present invention uses a DNS UPDATE message to report the load equivalent weight of the CSCF entity at regular time, so that the DNS Server can use the load equivalent weight when executing the load balancing strategy. It makes the disaster tolerance and load balancing in the IMS network be much easier to use and extend, thus reducing the load of the IMS network.

Abstract: Disclosed is a method for distinguishing the immunorepertoires of normal, healthy individuals from those of individuals who have symptomatic and/or non-symptomatic disease.

Abstract: A drive circuit comprising: a direct current power source; a control unit for supplying control signals; a power switch topology comprising a first switch and a second switch each having an input terminal, an output terminal, and a control terminal, the input terminals being respectively connected to the power source, the control terminals being connected to the control unit for receiving the control signals there from, the output terminals being connected to a node; and an inductance connected with a capacitive load in series between the node and the power source, wherein the control signals control the switches to alternately conduct to thereby cause the node to output a pulse signal.

Abstract: Disclosed is a method for amplifying and detecting polynucleotides which can provide sensitive, specific detection of multiple targets from a clinical specimen within a relatively short time.

Abstract: Disclosed is a method for amplifying and detecting polynucleotides which can provide sensitive, specific detection of multiple targets from a clinical specimen within a relatively short time.

Abstract: The present disclosure is a cassette for PCR amplification and detection of DNA amplicons, the cassette having a cassette pipette which may be moved vertically and horizontally within the cassette, at least one reagent chamber, and at least one detector, such as a microarray, for the detection of DNA amplified during the PCR amplification steps.

Abstract: Described is a novel method for the detection of ncRNA molecules. The disclosed method is especially useful for the detection miRNA and siRNA. The method can be used to generate a profile of the ncRNA molecules present in a sample. In addition, using the methods of the present disclosure a ncRNA signature for a given disease or condition can be created. The ncRNA signature can be used for diagnostic purposes, therapeutic purposes and drug discovery purposes, as well as other uses.

Abstract: Disclosed is an isolated strain of a previously unknown Staphylococcus, Staphylococcus pseudolugdunensis. Also disclosed are the sequences of the S. pseudolugdunensis tuf gene and 16s rRNA and methods for distinguishing S. pseudolugdunensis from other staphylococcal species.

Abstract: Disclosed is a method for isolating, and thereby concentrating, microorganisms from mammalian blood. The method may be used in conjunction with PCR amplification of the DNA/RNA of the microorganism(s) to identify pathogenic microorganisms within the blood without requiring that those organisms be cultured as a first step in the identification process.

Abstract: A drive circuit comprising: a direct current power source; a control unit for supplying control signals; a power switch topology comprising a first switch and a second switch each having an input terminal, an output terminal, and a control terminal, the input terminals being respectively connected to the power source, the control terminals being connected to the control unit for receiving the control signals there from, the output terminals being connected to a node; and an inductance connected with a capacitive load in series between the node and the power source, wherein the control signals control the switches to alternately conduct to thereby cause the node to output a pulse signal.

Abstract: Disclosed is a method for amplifying RNA and/or DNA from immune cell populations and using the amplified products to produce an immune response profile and evaluate the possible correlation between a normal or abnormal immune response and the development of a disease such as an autoimmune disease, cancer, diabetes, or heart disease.

Abstract: Disclosed is a method for amplifying and detecting polynucleotides which can provide sensitive, specific detection of multiple targets from a clinical specimen within a relatively short time.