Abstract: A navigation system is disclosed which comprises a tracker having a trackable feature, a localizer configured to track states of the trackable feature within a field of view, an illumination assembly operable to direct light within the field of view, and a controller coupled to the localizer and to the illumination assembly. The controller is configured to receive the tracked states from the localizer, to determine a status condition of the tracker based on the tracked states received from the localizer, and to control the illumination assembly to direct light at the tracker such that light reflected by the tracker from the illumination assembly is visible to a user of the navigation system to communicate the status condition of the tracker.

Abstract: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.

Abstract: Protein arrays and their use to assay, in a parallel fashion, the protein products of highly homologous or related DNA coding sequences and described. By highly homologous or related it is meant those DNA coding sequences which share a common sequence and which differ only by one or more naturally occurring mutations such as single nucleotide polymorphisms, deletions or insertions, or those sequences which are considered to be haplotypes. Such highly homologous or related DNA coding sequences are generally naturally occurring variants of the same gene. Arrays according to the invention have two or more individual proteins deposited in a spatially defined pattern on a surface in a form whereby a property such as an activity or function of the proteins can be investigated or assayed in parallel by interrogation of the array.

Abstract: There are provided methods and systems for making or repairing a specified part. For example, there is provided a method for creating an optimized manufacturing process to make or repair the specified part. The method includes receiving data from a plurality of sources, the data including as-designed, as-manufactured, as-simulated, and as-tested data relative to one or more parts similar to the specified part. The method includes updating, in real time, a surrogate model corresponding with a physics-based model of the specified part, wherein the surrogate model forms a digital twin of the specified part. The method includes further updating the surrogate model with a model of manufactured variance associated with at least one of inspection and in-operation data of a similar part. The method includes executing, based on the digital twin, the optimized manufacturing process to either repair or make the specified part.

Abstract: There are provided methods and systems for making or repairing a specified part. For example, there is provided a method for creating an optimized manufacturing process to make or repair the specified part. The method includes receiving data from a plurality of sources, the data including as-designed, as-manufactured, as-simulated, as-inspected, as-operated, and as-tested data relative to one or more parts similar to the specified part. The method includes updating, in real time, a surrogate model corresponding with a physics-based model of the specified part, wherein the surrogate model forms a digital twin of the specified part. The method includes generating a prognostic model of predicted performance of the specified part based on the surrogate model and based on one or more characteristics of at least one of an additive and a reductive manufacturing process. The method includes executing, based on the digital twin, the optimized manufacturing process to either repair or make the specified part.

Abstract: The present invention relates to a sequencing method which allows for increased rates of sequencing and an increase in the density of sequencing data. The system may be based on next generation sequencing methods such as sequencing by synthesis (SBS) but uses multiple primers bound at different positions on the same nucleic acid strand.

Abstract: There are provided methods and systems for making or repairing a specified part. For example, there is provided a method for creating a manufacturing process to make or repair the specified part. The method includes receiving data from a plurality of sources, the data including as-designed, as-manufactured, as-simulated, as-operated, as-inspected, and as-tested data relative to one or more parts similar to the specified part. The method includes updating, in real time, a surrogate model corresponding with a physics-based model of the specified part, wherein the surrogate model forms a digital twin of the specified part. The method includes generating a multi-variant distribution including component performance and manufacturing variance, the manufacturing variance being associated with at least one of an additive manufacturing process step and a reductive manufacturing process step.

Abstract: There are provided methods and systems for making or repairing a specified part. For example, there is provided a method for creating an optimized manufacturing process to make or repair the specified part. The method includes receiving by a system configured to make or repair the specified part and from a machine communicatively coupled with the system, a set of sensor data and/or inspection data associated with at least one of an additive and a reductive manufacturing or repair process or with at least one of a pre-treatment and a post-treatment step. The method includes creating an optimized manufacturing process to make or repair the specified part, the creating including. The method includes updating, in real time, a surrogate model corresponding with a physics-based model of the specified part, wherein the surrogate model forms a digital twin of the specified part. The method includes further updating the surrogate model with the sensor data and/or inspection data.

Abstract: The invention provides methods for amplifying nucleic acids, particularly methods for reducing density-dependent GC bias and for reducing nucleic acid damage in a bridge amplification of a nucleic acid template. The invention also provides methods for evaluating the effect of reagents and/or additives on nucleic acid damage during bridge amplification of nucleic acid template strands. The methods are suited to solid phase amplification, for example, utilizing flow cells.

Abstract: The present disclosure relates to a data storage system, and processes and computer programs for such data storage system, for example including processing of: managing one or more metadata tree structures for storing data to one or more storage devices of the data storage system in units of blocks, each metadata tree structure including a root node pointing directly and/or indirectly to blocks, and a leaf tree level having one or more direct nodes pointing to blocks, and optionally including one or more intermediate tree levels having one or more indirect nodes pointing to indirect nodes and/or direct nodes of the respective metadata tree structure; maintaining the root node and/or nodes of at least one tree level of each of at least one metadata structure in a cache memory; and managing I/O access to data based on the one or more metadata structures, including obtaining the root node and/or nodes of the at least one tree level of the metadata structure maintained in the cache memory from the cache memory an

Abstract: An electrical connector for a glazing including a first connector foot and a second connector foot for soldering to the glazing, and a bridge portion comprising sheet metal having a thickness in a specified range linking the first connector foot and second connector foot. The first connector foot and the second connector foot each are shaped so that most of the perimeter of each foot is curved, the first connector foot and the second connector foot are connected to the bridge portion by a first neck portion and a second neck portion respectively, and the first neck portion and the second neck portion each have a narrower width than a width of the bridge portion.

Abstract: The present invention relates to compounds of formula I that function as inhibitors of RET (rearranged during transfection) kinase enzyme activity: wherein HET, X1, X2, X3, X4, integer a and R3 are each as defined herein. The present invention also relates to processes for the preparation of these compounds, to pharmaceutical compositions comprising them, and to their use in the treatment of proliferative disorders, such as cancer, as well as other diseases or conditions in which RET kinase activity is implicated.

Abstract: A method of compressing image data representing one or more images, the method including obtaining pixel data from the image data, the pixel data representing an pixel array within the one or more images, applying a transformation to the pixel data to determine a set of frequency coefficients indicative of frequency coefficients of the pixel array, encoding a selected subset of the set of frequency coefficients, generating an index indicative of the encoded frequency coefficients and generating compressed image data using the encoded frequency coefficients and the index.

Abstract: A monitoring system includes one or more units that are adapted monitor the radio frequency conditions of a facility or portion of the facility. The units include a packet sniffer and/or an RF spectrum analyzer. Sniffed packets and spectrum data are recorded and made available for analysis and display, either locally on the units or at one or more remote locations. The locations of the units are also gathered, thereby enabling correlation of the sniffed packets and/or RF spectrum data with locations within the facility. Real time RF conditions can thereby be gathered and used to improve the wireless communications within the facility and/or to ensure the wireless communication infrastructure of the facility is operating satisfactorily. The units may be person support apparatuses, such as beds, chairs, stretchers, cots, or the like.

Abstract: A device is provided that humidifies breathing gas. The device has a chamber with an inlet and an outlet. A chemical heater is positioned within the chamber and a trigger activates the heater. The chamber can be a conduit with an inner wall and an outer wall with a reservoir defined therebetween. The chemical heater can be positioned within the reservoir.

Abstract: The present disclosure relates to the field of molecular biology and more specifically to microarrays and methods.

Abstract: Systems and techniques for managing data in a relational database environment and a non-relational database environment. Data in the relational database environment that is static and to be maintained beyond a preselected threshold length of time is identified. The data is copied from the relational database and stored in the data the non-relational database. Access to the data is provided from the non-relational database via a user interface that accesses both the relational database and the non-relational database.

Abstract: Protective packaging, surgical kits, systems, and methods are described herein for assisting in determining whether a tool is properly installed on a surgical device. The protective packaging retains the tool and has trackable features defined relative to a tool center point of the tool. The trackable features have a predetermined state defined relative to the tool center point and the trackable features are configured to be detectable by a localizer to locate the tool center point. One or more controllers can compare the actual state of the tool center point with an expected state of the tool center point, which is based on an expected condition in which the tool is properly mounted to the surgical device. Based on the comparison, the one or more controllers can determine whether the tool is properly mounted to the surgical device.