Abstract: The invention provides methods for amplifying nucleic acids, particularly methods for reducing density-dependent GC bias and for reducing nucleic acid damage in a bridge amplification of a nucleic acid template. The invention also provides methods for evaluating the effect of reagents and/or additives on nucleic acid damage during bridge amplification of nucleic acid template strands. The methods are suited to solid phase amplification, for example, utilizing flow cells.

Abstract: A location detection system identifies the locations of medical devices such as patient support apparatuses and/or patient care devices within a medical facility. The devices communicate via a wired connection to one or more medical facility systems (e.g. nurse call system, computer network, etc.), and/or via a wireless connection to such systems. The location detection system automatically determines location information of the devices and communicates the location information so that the recipient of any outgoing alerts and/or other information sent from the devices is apprised of the location of the particular device sending the alert or other information. Caregivers are thereby able to respond to the correct location of an alert, and software systems such as EMR systems, admission discharge and transfer (ADT) systems, etc. are able to correlate transmitted device data with the location and/or patient assigned to that location.

Abstract: Techniques and architectures for data ingestion in an environment having a distributed data storage system. A request to import data into the environment. The data to be imported from an external source through an application programming interface (API). The request is analyzed to determine if the request corresponds to a request to import a large data load. The data is staged if the request is for the large data load. Data management jobs are created to cause the data to be stored in one or more nodes of the distributed data storage system. The data management jobs are transmitted to corresponding nodes in the distributed data storage system. The jobs are executed asynchronously to cause the data to be stored in the nodes.

Abstract: The present disclosure relates to a data storage system, and processes and computer programs for such data storage system, for example including processing of: managing one or more metadata tree structures for storing data to one or more storage devices of the data storage system in units of blocks, each metadata tree structure including a root node pointing directly and/or indirectly to blocks, and a leaf tree level having one or more direct nodes pointing to blocks, and optionally including one or more intermediate tree levels having one or more indirect nodes pointing to indirect nodes and/or direct nodes of the respective metadata tree structure; maintaining the root node and/or nodes of at least one tree level of each of at least one metadata structure in a cache memory; and managing I/O access to data based on the one or more metadata structures.

Abstract: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.

Abstract: A person support apparatus, such as a bed, cot, stretcher, chair, or the like, includes a frame, a support surface supported by the frame, a plurality of load cells that detect weight supported on the support surface, at least one A/D converter, and a controller. The load cells output analog signals that are converted to digital by the A/D converters. The controller switches a sampling rate of the A/D converters between at least first and second rates. The outputs from the load cells are forwarded to a plurality of signal acquisition nodes that include the A/D converters. The nodes are positioned at locations that minimize the length of travel of the analog signals, thereby reducing noise interference. Shivering, occupant absence/presence, vital signs, occupant movement, and/or other parameters are detected by the load cells.

Abstract: Systems and methods for rescheduling passengers are provided. One example aspect of the present disclosure is directed to a method for rescheduling passengers. The method includes receiving an indication that an event has disrupted a plurality of flights. The method includes identifying a plurality of itineraries. The method includes determining a plurality of sets for the itineraries. The method includes determining a plurality of operations plan alternatives based on the plurality of sets. The method includes determining one or more constraints from the plurality of sets of itineraries. The method includes applying the one or more constraints to the plurality of operations plan alternatives to determine an impact of the one or more constraints to the plurality of operations plan alternatives on the sets of itineraries. The method includes determining an operations plan comprising a schedule for the plurality of flights.

Abstract: Presented herein are recombinases for improved recombinase-mediated amplification of nucleic acids, such as a PCR-library having single-stranded adapter regions, on a patterned flow cell surface for improved cluster amplification, as well as methods and kits using the same.

Abstract: A method, medium, and system to receive a baseline airline schedule including details associated with at least one flight; optimize the baseline airline schedule in accordance with at least one specified optimization objective to generate an optimized airline schedule; evaluate a robustness of the optimized airline schedule based on an execution of a simulation based process to generate a set of quantitative metrics; and generate a record of the set of quantitative metrics.

Abstract: Presented herein are methods and compositions for enhancing utilization of surface primers during the surface amplification process. The methods are useful for surface amplification at improved densities. The methods and compositions provided herein enable creation of clusters which are brighter, but at the same densities as currently achieved using standard cluster amplification.

Abstract: A method of characterizing candidate agents including steps of (a) providing a library of candidate agents attached to nucleic acid tags; (b) contacting the library with a solid support to attach the candidate agents to the solid support, whereby an array of candidate agents is formed; (c) contacting the array with a screening agent, wherein one or more candidate agents in the array react with the screening agent; (d) detecting the array to determine that at least one candidate agent in the array reacts with the screening agent; (e) sequencing the nucleic acid tag to determine the tag sequences attached to candidate agents in the array; and (f) identifying the at least one candidate agent in the array that reacts with the screening agent based on the tag sequence that is attached to the at least one candidate agent.

Abstract: The present disclosure provides methods and systems for determining and/or characterizing one or more haplotypes and/or phasing of haplotypes in a nucleic acid sample. In particular, the disclosure provides methods for determining a haplotype and/or phasing of haplotypes in a nucleic acid sample by incorporating synthetic polymorphisms into fragments of a nucleic acid sample and utilizing the synthetic polymorphisms in determining one or more haplotypes and/or phasing of haplotypes.

Abstract: A patient support apparatus is provided, such as a bed, cot, stretcher, or the like, that includes a sensor adapted to detect movement of the occupant while the occupant is supported on the support apparatus. A controller monitors outputs from the sensor in response to a mobility assessment control being activated. After monitoring the outputs for a period of time, the controller generates a mobility score based on the outputs from the sensor. In some embodiments, the controller use outputs from the sensor to determine which region of a defined surface area the patient has moved to. The controller records these visited regions over a time period and uses them to generate the mobility assessment. The mobility assessment provides an objective measure of an important factor used in predicting a patient's risk of developing pressure ulcers.

Abstract: There is disclosed system for oxygenating a patient in relation to anaesthesia using high flow gas delivery. The system has a flow source, and a controller for determining oxygenation requirements of the patient before or during anaesthesia. A method of oxygenating a patient in relation to anaesthesia using high flow gas delivery is also disclosed. The method determines oxygenation requirements of the patient before or during anaesthesia.

Abstract: A patient support apparatus, such as a bed, cot, stretcher, or the like, uses the ability to communicate with a device via near field communication to determine that the device is physically proximate the support apparatus. The support apparatus uses this determination to associate itself with the device. In some instances, the associated device is a mattress positioned on the support apparatus; a footboard on the support apparatus; a medical device used with a patient supported on the support device; a nearby piece of furniture; another patient support apparatus; or an ID tag worn by a caregiver or patient, or attached to a piece of equipment. After the support apparatus and device are associated, they communicate information between each other using far field communication, which supports higher data transfer rates. Flux concentrators are added in some embodiments to extend and/or shape the range of near the field communication.

Abstract: A parts organizer receives small parts such as mechanical fasteners in bulk form and organizes a plurality of the parts in a common desired orientation. Unoriented parts are received on top of a parts holder and subsequently moved along an upper side of the parts holder by an agitator. A plurality of receptacles are formed in the parts holder and open on its upper side. Each receptacle is configured to hold one of the parts in the desired orientation and may include a lead-in feature to help reorient the parts as they encounter the receptacles during movement. A retaining wall operates to contain unheld parts at the upper side of the parts holder during a parts organizing step and to allow the agitator to clear unheld parts away from the upper side of the parts holder during a purge step.

Abstract: The present disclosure relates to the field of molecular biology and more specifically to methods for capturing and amplifying target polynucleotides on a solid surface.

Abstract: Presented herein are methods and compositions for concatenating template strands during the bridge amplification process. The methods are useful for surface amplification at improved densities. The methods and compositions provided herein enable creation of clusters which are brighter, but at the same densities as currently achieved using standard cluster amplification.

Abstract: Patient support apparatuses, such as beds, cots, stretchers, recliners, operating tables, and the like include wireless mesh network transceivers that enable them to communicate with each other, and other devices, via mesh networks and/or ad hoc networks. One or more additional wireless transceivers are included, such as WiFi transceivers, that enable direct communication with a healthcare facility network, such as an Ethernet. The mesh network communication between patient support apparatuses and other devices is used for any one or more of: extending the communication range of the existing IT infrastructure, efficiently routing data to the healthcare facility network, determining location of the patient support apparatuses and devices, transporting patient data from one patient support to the next as the patient moves, and for other aspects.

Abstract: A device is provided that humidifies breathing gas. The device has a chamber with an inlet and an outlet. A chemical heater is positioned within the chamber and a trigger activates the heater. The chamber can be a conduit with an inner wall and an outer wall with a reservoir defined therebetween. The chemical heater can be positioned within the reservoir.