Abstract: A truss system may include a plurality of beams. Each beam of the plurality of beams may have various cross-sectional sizes in a same plane. Additionally, the plurality of beams may have a geometric arrangement such that a structural weight at required strength level may be reduced to achieve optimal design.

Abstract: Provided herein are methods of predicting the effect of a concentration of a sensitizer on cell viability in a production bioreactor, methods of improving cell viability in a production bioreactor, methods of predicting cell viability in a production bioreactor, and methods for culturing a cell in a production bioreactor.

Abstract: This disclosure relates to the use of one or more proteins (e.g., globular proteins) having a low isoelectric point and/or a limited number (e.g., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (PCR).

Abstract: Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

Abstract: Example implementations relate to fluid feed holes. For example, a method of forming a fluid feed hole can include forming a via of a threshold size in a plurality of thin films of a fluid ejection die by removing a portion of the plurality of thin films, forming a fluid-attack-resistant material on the plurality of thin films and in the via, planarizing the fluid-attack-resistant material using chemical mechanical planarization (CMP), and forming the fluid feed hole by removing a portion of the planarized fluid-attack-resistant material in the via.

Abstract: Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.

Abstract: Sensors are configured to capture measurement data representing dissolved oxygen (DO) measurements of an environment and capacitance measurements of a medium of the environment. A memory includes computer-executable instructions. One or more processors are communicatively coupled to the one or more sensors and configured to execute the computer-executable instructions to carry out operations comprising: generating, using first measurement data captured by the one or more sensors, a model based on a relationship between the first set of DO measurements and the first set of capacitance measurements; receiving, from the one or more sensors, second measurement data; predicting, using the model and based on the new capacitance measurement, an expected DO measurement; determining whether to use the expected DO measurement or the new DO measurement; and controlling, a valve to cause the determined oxygen input amount to flow into the environment based on the expected DO measurement.

Abstract: According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.

Abstract: Disclosed herein are methods, systems, and devices for system event detection associated with power grid components. Methods include detecting, at a plurality of sensors, emissions from a system event associated with at least one winding of a power component. Methods also include determining, using a processor, a plurality of event parameters based, at least in part, on measurements made by the plurality of sensors, the event parameters identifying arrival times of the emissions at each of the plurality of sensors. Methods further include generating, using the processor, an output identifying an estimate of a position of the system event within the power component, the estimate being generated based, at least in part, on the arrival times identified by the plurality of event parameters.

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

Abstract: A surface emitting laser includes a conductive substrate, a metal bonding layer, a laser structure layer, an epitaxial semiconductor reflection layer, and an electrode layer. The laser structure layer has an epitaxial current-blocking layer having a current opening. Currents are transmitting through the current opening. The epitaxial current-blocking layer is grown by a semiconductor epitaxy process to confine the range of the currents to form electric fields.

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

Abstract: A front-folding golf cart may include a main rod, a handle, and a front wheel assembly rotatably attached to an end of the main rod. The rotation of the front wheel assembly, from an extended to retracted position, may occur in a forward-and-upward direction. The front wheel assembly may include a front wheel fork having a front wheel mounted thereto, a support chassis extending from the end of the main rod to a connecting portion of the front wheel fork, and a locking mechanism positioned therebetween at the connecting portion. The front wheel fork may fixedly attach to the support chassis, while the connection between the main rod and the supporting chassis may be capable of adjusting in situ. A lower bracket may attach to the support chassis. The front-folding golf cart may further include two rear wheels respectively connected to the main rod through two foldable rear wheel frames.

Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.

Abstract: A front-folding golf cart may include a main rod, a handle, and a front wheel assembly rotatably attached to an end of the main rod. The rotation of the front wheel assembly, from an extended to retracted position, may occur in a forward-and-upward direction. The front wheel assembly may include a front wheel fork having a front wheel mounted thereto, a support chassis extending from the end of the main rod to a connecting portion of the front wheel fork, and a locking mechanism positioned therebetween at the connecting portion. The front wheel fork may fixedly attach to the support chassis, while the connection between the main rod and the supporting chassis may be capable of adjusting in situ. A lower bracket may attach to the support chassis. The front-folding golf cart may further include two rear wheels respectively connected to the main rod through two foldable rear wheel frames.

Abstract: Provided herein are methods of predicting the effect of a concentration of a sensitizer on cell viability in a production bioreactor, methods of improving cell viability in a production bioreactor, methods of predicting cell viability in a production bioreactor, and methods for culturing a cell in a production bioreactor.

Abstract: This disclosure relates to the use of one or more proteins (e.g., globular proteins) having a low isoelectric point and/or a limited number (e.g., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (PCR).

Abstract: A surface emitting laser includes a conductive substrate, a metal bonding layer, a laser structure layer, an epitaxial semiconductor reflection layer, and an electrode layer. The laser structure layer has an epitaxial current-blocking layer having a current opening. Currents are transmitting through the current opening. The epitaxial current-blocking layer is grown by a semiconductor epitaxy process to confine the range of the currents to form electric fields.

Abstract: A surface emitting laser includes a conductive substrate, a metal bonding layer, a laser structure layer, an epitaxial semiconductor reflection layer, and an electrode layer. The laser structure layer has an epitaxial current-blocking layer having a current opening. Currents are only transmitting through the current opening. The epitaxial current-blocking layer is grown by a semiconductor epitaxy process to confine the range of the currents to form electric fields.