Abstract: Provided are a vesicular linker and a single-chain cyclic library constructed by using the linker. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantages of high throughput sequencing, high accuracy and simple operations.

Abstract: Provided is a method of constructing a sequencing library using an adaptor element in a bubble shape. The bubble-shaped adaptor is ligated to a DNA fragment respectively at the 3?-terminal and the 5?-terminal i.e., two bubble-shaped adaptors with same sequences are ligated in one step. The bubble-shaped adaptor-ligated product is then amplified with a primer complementary to the 3?-terminal of the long-chain nucleic acid of the bubble-shaped adaptor, so as to replace the non-paired sequence in the short-chain nucleic acid.

Abstract: Methods and systems are provided for processing speech inputs for a controlling one or more vehicle systems of a vehicle. In one embodiment, a method includes: receiving speech input from an audio channel; performing, by a processor, speech recognition on the speech input to obtain recognized results; determining, by a processor, an accuracy level of the audio channel based on a comparison of the recognized results and predictive phraseology; determining, by a processor, an integrity level of the audio channel based on situational awareness information; communicating the recognized results, accuracy level, and the integrity level to a vehicle system; and selectively using the recognized results by the vehicle system based on the accuracy level and the integrity level.

Abstract: The present invention provides a new and improved oligonucleotide detection method based on the nanopore technology with a probe containing a complementary sequence to the target oligonucleotide and a terminal extension at the probe's 3? terminus, 5? terminus, or both termini. The improved nanopore sensor with the probe enables sensitive, selective, and direct detection, differentiation and quantification of target oligonucleotides such as miRNAs. The inventive detection method may also be employed as a non-invasive and cost-effective diagnostic method for cancer detection based on miRNA levels in the patient's blood sample.

Abstract: A startup method and apparatus, and a computer system. When a CPU fails to start a system, receiving, by a startup apparatus, a backup area selection instruction, where the backup area selection instruction is used to instruct the startup apparatus to read a boot loader from a backup area of Nand-Flash; receiving a program read instruction sent by the CPU, where the program read instruction includes a primary area address of the boot loader in the Nand-Flash; acquiring a backup address of the boot loader in the Nand-Flash from a correspondence between primary and backup addresses according to the primary area address; reading the boot loader from the backup address; and sending the boot loader to the CPU, such that the CPU starts the system according to the boot loader.

Abstract: Provided are a vesicular linker and a single-chain cyclic library constructed by using the linker. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantages of high throughput sequencing, high accuracy and simple operations.

Abstract: Methods and systems are provided for processing speech inputs for a controlling one or more vehicle systems of a vehicle. In one embodiment, a method includes: receiving speech input from an audio channel; performing, by a processor, speech recognition on the speech input to obtain recognized results; determining, by a processor, an accuracy level of the audio channel based on a comparison of the recognized results and predictive phraseology; determining, by a processor, an integrity level of the audio channel based on situational awareness information; communicating the recognized results, accuracy level, and the integrity level to a vehicle system; and selectively using the recognized results by the vehicle system based on the accuracy level and the integrity level.

Abstract: The present invention provides a new and improved oligonucleotide detection method based on the nanopore technology with a probe containing a complementary sequence to the target oligonucleotide and a terminal extension at the probe's 3? terminus, 5? terminus, or both termini. The improved nanopore sensor with the probe enables sensitive, selective, and direct detection, differentiation and quantification of target oligonucleotides such as miRNAs. The inventive detection method may also be employed as a non-invasive and cost-effective diagnostic method for cancer detection based on miRNA levels in the patient's blood sample.

Abstract: Methods and charging apparatus are presented for charging cell capacitors of a multilevel power conversion system, in which an auxiliary charging source is selectively applied across DC bus terminals while one or more selected multilevel converter cells are alternately switched between a first state to bypass the selected cell or cells and a second state to charge the cell capacitor or capacitors of the selected cell via charging current from the auxiliary charging source.

Abstract: The present invention provides a new and improved oligonucleotide detection method based on the nanopore technology with a probe containing a complementary sequence to the target oligonucleotide and a terminal extension at the probe's 3? terminus, 5? terminus, or both termini. The improved nanopore sensor with the probe enables sensitive, selective, and direct detection, differentiation and quantification of target oligonucleotides such as miRNAs. The inventive detection method may also be employed as a non-invasive and cost-effective diagnostic method for cancer detection based on miRNA levels in the patient's blood sample.

Abstract: Multilevel converters and space vector modulation operating methods are presented in which fewer than all possible switching states are employed for space vector modulation of rectifier and inverter circuits to promote common mode voltage contribution cancellation or reduction therebetween and by using subsets of possible space vector modulation switching states associated with minimal positive or negative common mode voltages and in some cases using virtual vectors which have zero common mode voltages to eliminate the 3rd order harmonic components in common mode voltage as well as to reduce common mode voltage affects associated with multilevel inverter stages.

Abstract: The present invention provides a new and improved oligonucleotide detection method based on the nanopore technology with a probe containing a complementary sequence to the target oligonucleotide and a terminal extension at the probe's 3? terminus, 5? terminus, or both termini. The improved nanopore sensor with the probe enables sensitive, selective, and direct detection, differentiation and quantification of target oligonucleotides such as miRNAs. The inventive detection method may also be employed as a non-invasive and cost-effective diagnostic method for cancer detection based on miRNA levels in the patient's blood sample.

Abstract: Methods and charging apparatus are presented for charging cell capacitors of a multilevel power conversion system, in which an auxiliary charging source is selectively applied across DC bus terminals while one or more selected multilevel converter cells are alternately switched between a first state to bypass the selected cell or cells and a second state to charge the cell capacitor or capacitors of the selected cell via charging current from the auxiliary charging source.

Abstract: The present invention provides a new and improved oligonucleotide detection method based on the nanopore technology with a probe containing a complementary sequence to the target oligonucleotide and a terminal extension at the probe's 3? terminus, 5? terminus, or both termini. The improved nanopore sensor with the probe enables sensitive, selective, and direct detection, differentiation and quantification of target oligonucleotides such as miRNAs. The inventive detection method may also be employed as a non-invasive and cost-effective diagnostic method for cancer detection based on miRNA levels in the patient's blood sample.

Abstract: A versatile detection method is disclosed that utilizes a base-pair-specific inter-strand lock for genetic and epigenetic detection. Reagents, devices, etc., for implementing the method have also been discovered and/or developed. In certain embodiments, compounds have been identified to be able to specifically bind certain mismatched base pairs including T-T, U-T, and C-C base pair mismatches using either Hg2+ or Ag+. Such binding can strengthen the base-pair hybridization in orders of magnitude, forming a so-called reversible inter-strand lock that can greatly stabilize double-stranded nucleic acid fragments.

Abstract: A startup method and apparatus, and a computer system. When a CPU fails to start a system, receiving, by a startup apparatus, a backup area selection instruction, where the backup area selection instruction is used to instruct the startup apparatus to read a boot loader from a backup area of Nand-Flash; receiving a program read instruction sent by the CPU, where the program read instruction includes a primary area address of the boot loader in the Nand-Flash; acquiring a backup address of the boot loader in the Nand-Flash from a correspondence between primary and backup addresses according to the primary area address; reading the boot loader from the backup address; and sending the boot loader to the CPU, such that the CPU starts the system according to the boot loader.

Abstract: The invention discloses a touch detecting structure, an organic light emitting touch display device, a method of detecting a touch on a device and a method of manufacturing a device. The touch detecting structure includes: a first signal transmission line arranged on a first substrate of an organic light emitting touch display device; an insulating layer arranged on the first signal transmission line and having a via hole and a protrusion; a second signal transmission line located on the insulating layer and passing the top of the protrusion; a signal transmission terminal arranged on the lateral surface of the protrusion and having one end connected with the first signal transmission line through the via hole and the other end located on the top of the protrusion, and insulated from the second signal transmission line; and a cathode film arranged on a second substrate.

Abstract: An Organic Light-Emitting Diode (OLED) display device and a corresponding flexible printed circuit (FPC) are disclosed. The display device includes a first substrate and a second substrate arranged in opposition. In addition, frit is arranged on an inner side of the first substrate, and the display device also includes at least one bonding mark, where the bonding mark includes the frit, and at least one sub-bonding mark arranged on a surface of the first substrate. The at least one sub-bonding mark includes a first portion and a second portion arranged apart from each other, and the first portion and the second portion are arranged at an edge of the frit. The FPC includes at least one auxiliary bonding mark aligning the FPC to the OLED display device, where the at least one auxiliary bonding mark is aligned with the at least one sub-bonding mark of the OLED display device.

Abstract: Multilevel converters and space vector modulation operating methods are presented in which fewer than all possible switching states are employed for space vector modulation of rectifier and inverter circuits to promote common mode voltage contribution cancellation or reduction therebetween and by using subsets of possible space vector modulation switching states associated with minimal positive or negative common mode voltages and in some cases using virtual vectors which have zero common mode voltages to eliminate the 3rd order harmonic components in common mode voltage as well as to reduce common mode voltage affects associated with multilevel inverter stages.

Abstract: A method for activating a voice control function of a mobile phone communicating with and carried in a vehicle is provided. The method includes the following steps. A sensing unit arranged in the vehicle senses a real-time speed of the vehicle when the vehicle is moving. A processor of the vehicle determines whether a real-time speed of the vehicle exceeds a predefined speed according to the sensed real-time speed of the vehicle. The processor controls the mobile phone to activate the voice control function of the mobile phone if the speed of the vehicle exceeds a predefined speed. A vehicle using the method is also provided by the present disclosure.