Abstract: Healthy functional platelets are mass produced. A method for producing platelets, comprising: (1) a culture step of culturing megakaryocytes in a platelet producing medium in the presence of mechanical stress and platelet production promoting factors including MIF, NRDc, IGFBP2, TSP-1, PAI-1, and CCL5, and (2) a harvest step of harvesting the platelets obtained by the culture step; wherein the culture step comprises: (a) a step of promoting a release of the platelet production promoting factors from megakaryocytes by mechanical stress; and/or (b) a step of externally adding platelet production promoting factors including MIF, NRDc, and IGFBP2.

Abstract: To provide an authentication device and method for a security medium including a reflective volume hologram having a light source disposed on the front surface side of the reflective volume hologram so that light emitted therefrom is incident on the reflective volume hologram, a first observation device disposed in a pre-designed diffraction direction of the reflective volume hologram, and a second observation device disposed in a direction other than the pre-designed diffraction direction of the reflective volume hologram. Light including a pre-designed diffraction wavelength is emitted from the light source to be incident on the reflective volume hologram, and when the light amount observed in the first observation device is larger in the diffraction wavelength than in other wavelengths, and the light amount observed in the second observation device is smaller in the diffraction wavelength than in other wavelengths, it is determined that the reflective volume hologram is genuine.

Abstract: An object of the present invention is to provide a method of promoting polyploidization of megakaryocytes and thereby producing highly polyploidized megakaryocytes, a method of efficiently producing platelets from polyploidized megakaryocytes, and the like. The present invention provides a method of producing polyploidized megakaryocytes comprising a step of forcing expression of an apoptosis suppressor gene in megakaryocytes before polyploidization and culturing the resulting cells.

Abstract: A main object of the present invention is to provide a method of producing a volume hologram laminate which can regenerate a hologram image in an arbitrary wavelength by a simple process. To attain the object, the present invention provides a method of producing a volume hologram laminate using a volume hologram forming substrate which comprises: a substrate, a volume hologram layer formed on the substrate and containing a photopolymerizable material, a resin layer, formed on the substrate so as to contact to the volume hologram layer, containing a resin and a polymerizable compound, characterized in that the producing method comprises processes of: a hologram recording process to record a volume hologram to the volume hologram layer, a substance transit process of transiting the polymerizable compound to the volume hologram layer, and an after-treatment process of polymerizing the polymerizable compound.

Abstract: A display article includes a plurality of display areas. Display areas adjacent to each other differ in at least one of an average hue, an average brightness and an average chroma and a first object to be displayed is formed by a combination of the plurality of display areas. At least one of the display areas includes a Fourier transform hologram configured to convert incident ray from a point light source or a laser light source into a second object to be displayed.

Abstract: The present invention provides a method for culturing pluripotent stem cells, comprising the step of contacting pluripotent stem cells with laminin 421 or a fragment thereof, laminin 121 or a fragment thereof, or a combination thereof.

Abstract: Provided is a method for inducing mesoderm, comprising a step of bringing pluripotent stem cells into contact with bone morphogenetic protein 4 (BMP4) or CHIR for at least 3 days.

Abstract: The present invention provides a highly functional platelet production promoting agent which comprises one or a plurality of aryl hydrocarbon receptor (AhR) antagonists and one or a plurality of Rho-associated coiled-coil forming kinase (ROCK) inhibitors.

Abstract: The present invention provides a method for screening for platelet production promoters, the method including a step for selecting a candidate substance that significantly increases expression of TUBB1 as a platelet production promoter.

Abstract: The present invention provides: a platelet production promoter that contains one or more substances selected from the group consisting of Wnt inhibitors and FMS-like tyrosine kinase (FLT) inhibitors; and a platelet production method that uses this platelet production promoter.

Abstract: The present invention has an object of providing a method for producing specific cells by amplifying cells in a desired differentiation stage. The present invention provides a method for producing specific cells by inducing differentiation of cells, wherein an oncogene is forcibly expressed in cells in a desired differentiation stage to amplify the cells in the desired differentiation stage. The present invention also provides a method for producing specific cells, wherein oncogene-induced senescence (OIS) which is induced by the oncogene expressed in the cells in the desired differentiation stage is suppressed.

Abstract: In an image forming apparatus, when a processor starts to be supplied with a voltage in a first voltage range from a voltage generator, the processor executes start-up processing with an operation clock of a first frequency, and transmits a voltage adjustment instruction to the voltage generator. On receiving the voltage adjustment instruction, the voltage generator performs voltage adjustment to make the voltage supplied to the processor a voltage in a second voltage range. On completion of the voltage adjustment, the voltage generator transmits a completion notice to the processor. When communication related to the voltage adjustment does not end normally, the processor keeps the frequency of the operation clock at the first frequency to execute processing; thereafter based on the operation clock of the first frequency.

Abstract: Provided is a method for producing a stem cell clone, which comprises the steps of: (i) introducing into stem cells an exogenous gene associated with induction of differentiation into somatic cells; (ii) inducing differentiation of the stem cells, introduced with an exogenous gene, into the somatic cells; (iii) dedifferentiating the differentiation-induced somatic cells; and (iv) isolating stem cells having the exogenous gene incorporated into a chromosome thereof from a colony of the stem cells formed in step (iii).

Abstract: The present invention provides a method for producing megakaryocytes that have an increased capacity to produce platelets, the method comprising a step of culturing, under conditions that cause the death of cells that do not express a gene that is specifically expressed by megakaryocytes, cells that have the capacity to differentiate into megakaryocytes.

Abstract: An object of the present invention is to provide a method of promoting polyploidization of megakaryocytes and thereby producing highly polyploidized megakaryocytes, a method of efficiently producing platelets from polyploidized megakaryocytes, and the like. The present invention provides a method of producing polyploidized megakaryocytes comprising a step of forcing expression of an apoptosis suppressor gene in megakaryocytes before polyploidization and culturing the resulting cells.

Abstract: A display article (10) includes a plurality of display areas (12, and 13a to 13c). Display areas adjacent to each other differ in at least one of an average hue, an average brightness and an average chroma and a first object to be displayed (21) is formed by a combination of the plurality of display areas (12, and 13a to 13c). At least one of the display areas (12, 13a to 13c) includes a Fourier transform hologram (20R, 20Y) configured to convert incident ray from a point light source or a laser light source into a second object to be displayed.

Abstract: To provide an authentication device and method for a security medium including a reflective volume hologram, capable of easily performing authentication determination and a security medium including the reflective volume hologram. An authenticity determination device 1 for a security medium 10 including a reflective volume hologram 2 includes a light source L disposed on the front surface side of the reflective volume hologram 2 so that light emitted therefrom is incident on the reflective volume hologram 2, a first observation device 11 disposed in a pre-designed diffraction direction of the reflective volume hologram 2, and a second observation device 12 disposed in a direction other than the pre-designed diffraction direction of the reflective volume hologram 2.

Abstract: The invention relates to a holographic viewing device that enable printing or the like to be directly applied to a transmission hologram substrate without recourse to any frame for supporting and reinforcing a transmission hologram, thereby simplifying construction while enhancing aesthetic and decorative attributes, and a holographic viewing card incorporating it. The holographic viewing device enables a given image or message to be viewed near the positions of point light sources upon viewing the point light sources through a hologram, and comprises a transparent substrate 41, a hologram-formation layer 42 and a printing layer 45. The hologram-formation layer 42 may be any one of a phase type diffractive optical element having a relief structure 43 on its surface, a phase type diffractive optical element having a refractive index profile in its layer, and an amplitude type diffractive optical element having a transmittance profile in its layer.

Abstract: An object of the present invention is to provide a method of promoting polyploidization of megakaryocytes and thereby producing highly polyploidized megakaryocytes, a method of efficiently producing platelets from polyploidized megakaryocytes, and the like. The present invention provides a method of producing polyploidized megakaryocytes comprising a step of forcing expression of an apoptosis suppressor gene in megakaryocytes before polyploidization and culturing the resulting cells.

Abstract: The present invention is to provide: a photosensitive composition for volume hologram recording and a photosensitive substrate for volume hologram recording, which provide increased sensitivity during hologram recording and a high refractive index modulation amount, and which do not inhibit the decolorization of the sensitizing dye; and a volume hologram recorded medium which provides high-luminance hologram images with increased productivity.