Patents by Inventor Lakmal Jayasinghe

Lakmal Jayasinghe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220283141
    Abstract: The invention relates to a novel method of producing hetero-oligomeric pores. The invention also relates to hetero-oligomeric pores produced using the method and polynucleotide characterisation using the hetero-oligomeric pores.
    Type: Application
    Filed: March 14, 2022
    Publication date: September 8, 2022
    Applicant: Oxford Nanopore Technologies PLC
    Inventors: Lakmal Jayasinghe, John Joseph Kilgour, Neil Roger Wood
  • Publication number: 20220162264
    Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.
    Type: Application
    Filed: March 18, 2021
    Publication date: May 26, 2022
    Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit Brussel
    Inventors: Han Remaut, Sander Van Der Verren, Nani Van Gerven, Lakmal Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Jordan, John Joseph Kilgour
  • Publication number: 20220154269
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterization using CsgG.
    Type: Application
    Filed: March 8, 2021
    Publication date: May 19, 2022
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Publication number: 20220135956
    Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
    Type: Application
    Filed: October 13, 2021
    Publication date: May 5, 2022
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Andrew John Heron, Rebecca Victoria Bowen, Mark Bruce, Lakmal Jayasinghe, Joseph Hargreaves Lloyd, Szabolcs Soeroes, Elizabeth Jayne Wallace, Christopher Peter Youd
  • Publication number: 20220119879
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterization using CsgG.
    Type: Application
    Filed: October 26, 2021
    Publication date: April 21, 2022
    Applicant: Oxford Nanopore Technologies PLC
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Patent number: 11307192
    Abstract: The invention relates to a novel method of producing hetero-oligomeric pores. The invention also relates to hetero-oligomeric pores produced using the method and polynucleotide characterisation using the hetero-oligomeric pores.
    Type: Grant
    Filed: February 17, 2016
    Date of Patent: April 19, 2022
    Assignee: Oxford Nanopore Technologies PLC
    Inventors: Lakmal Jayasinghe, John Joseph Kilgour, Neil Roger Wood
  • Publication number: 20220064230
    Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.
    Type: Application
    Filed: July 26, 2021
    Publication date: March 3, 2022
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Mark Bruce, Luke McNeill, Ramiz Iqbal Nathani, Pratik Raj Singh, Neil Roger Wood, Stephen Robert Young
  • Publication number: 20220064722
    Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
    Type: Application
    Filed: June 23, 2021
    Publication date: March 3, 2022
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Clarke
  • Publication number: 20210395811
    Abstract: The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.
    Type: Application
    Filed: April 30, 2021
    Publication date: December 23, 2021
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White
  • Patent number: 11186868
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.
    Type: Grant
    Filed: March 2, 2017
    Date of Patent: November 30, 2021
    Assignee: Oxford Nanopore Technologies PLC
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Patent number: 11180741
    Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
    Type: Grant
    Filed: October 6, 2015
    Date of Patent: November 23, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Andrew John Heron, Rebecca Victoria Bowen, Mark Bruce, Lakmal Jayasinghe, Joseph Hargreaves Lloyd, Szabolcs Soeroes, Elizabeth Jayne Wallace, Christopher Peter Youd
  • Publication number: 20210324020
    Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.
    Type: Application
    Filed: December 1, 2020
    Publication date: October 21, 2021
    Applicant: Oxford Nanopore Technologies Limited
    Inventors: Mark Bruce, James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Publication number: 20210317520
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.
    Type: Application
    Filed: March 29, 2021
    Publication date: October 14, 2021
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Publication number: 20210292376
    Abstract: The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.
    Type: Application
    Filed: March 8, 2021
    Publication date: September 23, 2021
    Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • Publication number: 20210284696
    Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.
    Type: Application
    Filed: March 18, 2021
    Publication date: September 16, 2021
    Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit Brussel
    Inventors: Han Remaut, Sander Van Der Verren, Nani Van Gerven, Lakmal Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Jordan, John Joseph Kilgour
  • Publication number: 20210269872
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterization using CsgG.
    Type: Application
    Filed: March 8, 2021
    Publication date: September 2, 2021
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Patent number: 11104709
    Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.
    Type: Grant
    Filed: April 6, 2017
    Date of Patent: August 31, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Mark John Bruce, Luke McNeill, Ramiz Iqbal Nathani, Pratik Raj Singh, Neil Roger Wood, Stephen Robert Young
  • Patent number: 11078530
    Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
    Type: Grant
    Filed: September 12, 2018
    Date of Patent: August 3, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
  • Patent number: 11034734
    Abstract: The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.
    Type: Grant
    Filed: July 25, 2019
    Date of Patent: June 15, 2021
    Assignees: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • Patent number: 11021747
    Abstract: The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.
    Type: Grant
    Filed: September 19, 2019
    Date of Patent: June 1, 2021
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White