Patents by Inventor Lakmal Jayasinghe
Lakmal Jayasinghe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11965183Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: GrantFiled: October 13, 2021Date of Patent: April 23, 2024Assignee: Oxford Nanopore Technologies PLCInventors: Andrew John Heron, Rebecca Victoria Bowen, Mark Bruce, Lakmal Jayasinghe, Joseph Hargreaves Lloyd, Szabolcs Soeroes, Elizabeth Jayne Wallace, Christopher Peter Youd
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Patent number: 11945840Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: GrantFiled: March 18, 2021Date of Patent: April 2, 2024Assignees: VIB VZW, Vrije Universiteit Brussel, Oxford Nanopore Technologies PLCInventors: Han Remaut, Sander Van Der Verren, Nani Van Gerven, Lakmal Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Jordan, John Joseph Kilgour
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Patent number: 11939359Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.Type: GrantFiled: July 26, 2021Date of Patent: March 26, 2024Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Jayasinghe, Mark Bruce, Luke McNeill, Ramiz Iqbal Nathani, Pratik Raj Singh, Neil Roger Wood, Stephen Robert Young
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Patent number: 11859247Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: GrantFiled: June 23, 2021Date of Patent: January 2, 2024Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Clarke
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Patent number: 11845780Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.Type: GrantFiled: December 1, 2020Date of Patent: December 19, 2023Assignee: Oxford Nanopore Technologies PLCInventors: Mark Bruce, James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
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Patent number: 11840556Abstract: Provided herein relate to modified or mutant forms of secretin and compositions comprising the same. In particular, the modified or mutant forms of secretin permits efficient capture and/or translocation of an analyte through the modified or mutant secretin nanopores. Methods for using unmodified secretin or the modified or mutant forms of secretin and compositions, for example, for characterizing an analyte, e.g., a target polynucleotide, are also provided.Type: GrantFiled: February 12, 2018Date of Patent: December 12, 2023Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh
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Publication number: 20230227799Abstract: The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: ApplicationFiled: October 18, 2022Publication date: July 20, 2023Applicant: Oxford Nanopore Technologies PLCInventors: Andrew John Heron, James Anthony Clarke, Ruth Moysey, Elizabeth Jayne Wallace, Mark John Bruce, Lakmal Jayasinghe, Domenico Caprotti, Szabolcs Soeroes, Luke McNeill, David Antoni Alves, Rebecca Victoria Bowen, John Milton
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Publication number: 20230084931Abstract: Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.Type: ApplicationFiled: August 3, 2022Publication date: March 16, 2023Applicant: Oxford Nanopore Technologies PLCInventors: James Anthony Clarke, James White, Richard Muscat, Jessica Mary May Knott, Ramiz Iqbal Nathani, Andrew John Heron, Mark John Bruce, Lakmal Jayasinghe, Domenico Caprotti, David Jackson Stoddart, Rebecca Victoria Bowen, Christopher James Wright, Paul Richard Moody
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Publication number: 20230012471Abstract: The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.Type: ApplicationFiled: April 30, 2021Publication date: January 12, 2023Applicant: Oxford Nanopore Technologies Ltd.Inventors: Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White
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Patent number: 11525126Abstract: The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: GrantFiled: October 6, 2020Date of Patent: December 13, 2022Assignee: Oxford Nanopore Technologies PLCInventors: Andrew Heron, Anthony Clarke, Ruth Moysey, Elizabeth Jayne Wallace, Mark John Bruce, Lakmal Jayasinghe, Domenico Caprotti, Szabolcs Soeroes, Luke McNeill, David Antoni Alves, Rebecca Victoria Bowen, John Milton
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Publication number: 20220283141Abstract: The invention relates to a novel method of producing hetero-oligomeric pores. The invention also relates to hetero-oligomeric pores produced using the method and polynucleotide characterisation using the hetero-oligomeric pores.Type: ApplicationFiled: March 14, 2022Publication date: September 8, 2022Applicant: Oxford Nanopore Technologies PLCInventors: Lakmal Jayasinghe, John Joseph Kilgour, Neil Roger Wood
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Publication number: 20220162264Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: ApplicationFiled: March 18, 2021Publication date: May 26, 2022Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit BrusselInventors: Han Remaut, Sander Van Der Verren, Nani Van Gerven, Lakmal Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Jordan, John Joseph Kilgour
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Publication number: 20220154269Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterization using CsgG.Type: ApplicationFiled: March 8, 2021Publication date: May 19, 2022Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
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Publication number: 20220135956Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: ApplicationFiled: October 13, 2021Publication date: May 5, 2022Applicant: Oxford Nanopore Technologies Ltd.Inventors: Andrew John Heron, Rebecca Victoria Bowen, Mark Bruce, Lakmal Jayasinghe, Joseph Hargreaves Lloyd, Szabolcs Soeroes, Elizabeth Jayne Wallace, Christopher Peter Youd
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Publication number: 20220119879Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterization using CsgG.Type: ApplicationFiled: October 26, 2021Publication date: April 21, 2022Applicant: Oxford Nanopore Technologies PLCInventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
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Patent number: 11307192Abstract: The invention relates to a novel method of producing hetero-oligomeric pores. The invention also relates to hetero-oligomeric pores produced using the method and polynucleotide characterisation using the hetero-oligomeric pores.Type: GrantFiled: February 17, 2016Date of Patent: April 19, 2022Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Jayasinghe, John Joseph Kilgour, Neil Roger Wood
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Publication number: 20220064722Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: ApplicationFiled: June 23, 2021Publication date: March 3, 2022Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Clarke
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Publication number: 20220064230Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.Type: ApplicationFiled: July 26, 2021Publication date: March 3, 2022Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, Mark Bruce, Luke McNeill, Ramiz Iqbal Nathani, Pratik Raj Singh, Neil Roger Wood, Stephen Robert Young
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Publication number: 20210395811Abstract: The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.Type: ApplicationFiled: April 30, 2021Publication date: December 23, 2021Applicant: Oxford Nanopore Technologies Ltd.Inventors: Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White
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Patent number: 11186868Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.Type: GrantFiled: March 2, 2017Date of Patent: November 30, 2021Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace