Patents by Inventor Lakmal Jayasinghe
Lakmal Jayasinghe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20180179500Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: ApplicationFiled: October 6, 2015Publication date: June 28, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventors: Andrew John Heron, Rebecca Victoria Bowen, Mark Bruce, Lakmal Jayasinghe, Joseph Hargreaves Lloyd, Szabolcs Soeroes, Elizabeth Jayne Wallace, Christopher Peter Youd
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Publication number: 20180148481Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.Type: ApplicationFiled: September 1, 2015Publication date: May 31, 2018Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit BrusselInventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
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Publication number: 20180095066Abstract: The invention relates to a novel method of producing hetero-oligomeric pores. The invention also relates to hetero-oligomeric pores produced using the method and polynucleotide characterisation using the hetero-oligomeric pores.Type: ApplicationFiled: February 17, 2016Publication date: April 5, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, John Joseph Kilgour, Neil Roger Wood
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Patent number: 9885078Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: GrantFiled: August 8, 2014Date of Patent: February 6, 2018Assignee: Oxford Nanopore Technologies LimitedInventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
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Publication number: 20180030526Abstract: The invention relates to hetero-oligomeric pores derived from Msp. The invention also relates to polynucleotide characterisation using the hetero-oligomeric pores.Type: ApplicationFiled: February 17, 2016Publication date: February 1, 2018Applicant: Oxford Nanopore Technologies Ltd.Inventors: Clive Gavin Brown, James Anthony Clarke, Richard George Hambley, Andrew John Heron, Lakmal Jayasinghe, John Milton, Jonathan Bankes Pugh, Elizabeth Jayne Wallace
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Publication number: 20170356037Abstract: The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterisation using Msp.Type: ApplicationFiled: May 1, 2015Publication date: December 14, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
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Publication number: 20170335384Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: ApplicationFiled: August 3, 2017Publication date: November 23, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
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Publication number: 20170306398Abstract: The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterisation using Msp.Type: ApplicationFiled: October 6, 2015Publication date: October 26, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Andrew John Heron, James White
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Patent number: 9797009Abstract: The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: GrantFiled: July 18, 2013Date of Patent: October 24, 2017Assignee: Oxford Nanopore Technologies LimitedInventors: Andrew Heron, James Clarke, Ruth Moysey, Elizabeth Jayne Wallace, Mark Bruce, Lakmal Jayasinghe, Domenico Caprotti, Szabolcs Soeroes, Luke McNeill, Mihaela Misca
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Publication number: 20170283470Abstract: The invention relates to mutant forms of the outer membrane located lipoprotein CsgG, in particular, modifications at one or more of positions Tyr51; Asn55; and Phe56. The invention also relates to analyte detection and characterisation using said mutant CsgG.Type: ApplicationFiled: September 1, 2015Publication date: October 5, 2017Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit BrusselInventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
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Patent number: 9777049Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterization using lysenin.Type: GrantFiled: March 15, 2013Date of Patent: October 3, 2017Assignee: Oxford Nanopore Technologies Ltd.Inventors: Mark Bruce, James Clarke, Andrew Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
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Publication number: 20170253923Abstract: The invention relates to a new method of characterising FIG. 3 a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.Type: ApplicationFiled: October 19, 2015Publication date: September 7, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White
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Patent number: 9751915Abstract: The invention relates to mutant forms of Msp. The invention also relates to nucleic acid characterization using Msp.Type: GrantFiled: February 10, 2012Date of Patent: September 5, 2017Assignee: Oxford Nanopore Technologies Ltd.Inventors: James Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James White
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Publication number: 20170058338Abstract: The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein. The invention also relates to improved transmembrane pores and polynucleotide binding proteins.Type: ApplicationFiled: May 1, 2015Publication date: March 2, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Clive Gavin Brown, James White
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Publication number: 20170058337Abstract: The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterisation using Msp.Type: ApplicationFiled: May 1, 2015Publication date: March 2, 2017Applicant: Oxford Nanopore Technologies Ltd. Oxford Nanapore TEchnologies LomitedInventors: James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
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Publication number: 20170002406Abstract: The invention relates to new methods of controlling the movement of polynucleotides through transmembrane pores. The invention also relates to new methods of characterising target polynucleotides using helicases.Type: ApplicationFiled: September 10, 2014Publication date: January 5, 2017Applicant: Oxford Nanopore Technologies Ltd.Inventors: Rebecca Victoria Bowen, Clive Gavin Brown, Mark Bruce, Andrew John Heron, Elizabeth Jayne Wallace, James White, Joseph Hargreaves Lloyd, David Antoni Alves, Domenico Caprotti, Lakmal Jayasinghe, Luke McNeill, John Milton, Antonino Puglisi, Szabolcs Soeroes
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Patent number: 9447152Abstract: The invention relates to a mutant ?-hemolysin (?-HL) pore which is useful for detecting one or more nucleotides by stochastic sensing. The pore is particularly useful for sequencing DNA or RNA. A molecular adaptor that allows detection of the nucleotide(s) is covalently attached to the pore. The pore is specifically modified to facilitate positioning of the adaptor and may be modified to facilitate covalent attachment.Type: GrantFiled: July 6, 2009Date of Patent: September 20, 2016Assignee: Oxford Nanopore Technologies LimitedInventors: James Anthony Clarke, Lakmal Jayasinghe, Terence Reid, John Hagan Pryce Bayley
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Publication number: 20160076092Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: ApplicationFiled: September 18, 2015Publication date: March 17, 2016Inventors: Lakmal JAYASINGHE, John Hagan Pryce BAYLEY, Stephen CHELEY, Brian MCKEOWN, James WHITE, James Anthony CLARKE
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Patent number: 9222082Abstract: The invention provides method of covalently coupling two or more moieties, the method comprising: (a) providing a first moiety having covalently attached thereto (i) at least one first linker comprising a first hybridizable region and (ii) at least one first group capable of forming a covalent bond; (b) providing a second moiety having covalently attached thereto (i) at least one second linker comprising a second hybridizable region capable of hybridizing to the first hybridizable region and (ii) at least a second group capable of forming a covalent bond with the first group; (c) contacting the first and second moieties under conditions that allow the first and second hybridizable regions to hybridize and link the moieties; and (d) exposing the linked moieties to conditions that allow the formation of a covalent bond between the first and second groups.Type: GrantFiled: January 29, 2010Date of Patent: December 29, 2015Assignee: Oxford Nanopore Technologies LimitedInventors: Lakmal Jayasinghe, John Milton, Luke McNeill, James Anthony Clarke, James White, Ruth Moysey
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Publication number: 20150218629Abstract: The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.Type: ApplicationFiled: July 18, 2013Publication date: August 6, 2015Inventors: Andrew Heron, James Clarke, Ruth Moysey, Elizabeth Jayne Wallace, Mark Bruce, Lakmal Jayasinghe, Domenico Caprotti, Szabolcs Soeroes, Luke McNeill, Mihaela Misca