Patents by Inventor Lakmal Jayasinghe

Lakmal Jayasinghe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Mutant pores
    Patent number: 10266885
    Abstract: The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterization using Msp.
    Type: Grant
    Filed: October 6, 2015
    Date of Patent: April 23, 2019
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Andrew John Heron, James White
  • MUTANT PORE
    Publication number: 20190071721
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.
    Type: Application
    Filed: March 2, 2017
    Publication date: March 7, 2019
    Applicant: Oxford Nanopore Technologies Limited
    Inventors: Lakmal JAYASINGHE, Elizabeth Jayne WALLACE
  • ENZYME-PORE CONSTRUCTS
    Publication number: 20190002972
    Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
    Type: Application
    Filed: September 12, 2018
    Publication date: January 3, 2019
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
  • Mutant pores
    Patent number: 10167503
    Abstract: The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterization using Msp.
    Type: Grant
    Filed: May 1, 2015
    Date of Patent: January 1, 2019
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Enzyme-pore constructs
    Patent number: 10077471
    Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
    Type: Grant
    Filed: September 18, 2015
    Date of Patent: September 18, 2018
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
  • ENZYME CONSTRUCT
    Publication number: 20180230526
    Abstract: The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
    Type: Application
    Filed: September 14, 2017
    Publication date: August 16, 2018
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Andrew John Heron, James Anthony Clarke, Ruth Moysey, Elizabeth Jayne Wallace, Mark Bruce, Lakmal Jayasinghe, Domenico Caprotti, Szabolcs Soeroes, Luke McNeill, Mihaela Misca
  • MUTANT LYSENIN PORES
    Publication number: 20180208632
    Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.
    Type: Application
    Filed: August 31, 2017
    Publication date: July 26, 2018
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Mark Bruce, James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • MODIFIED ENZYMES
    Publication number: 20180179500
    Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
    Type: Application
    Filed: October 6, 2015
    Publication date: June 28, 2018
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Andrew John Heron, Rebecca Victoria Bowen, Mark Bruce, Lakmal Jayasinghe, Joseph Hargreaves Lloyd, Szabolcs Soeroes, Elizabeth Jayne Wallace, Christopher Peter Youd
  • MUTANT CSGG PORES
    Publication number: 20180148481
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.
    Type: Application
    Filed: September 1, 2015
    Publication date: May 31, 2018
    Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • METHOD
    Publication number: 20180095066
    Abstract: The invention relates to a novel method of producing hetero-oligomeric pores. The invention also relates to hetero-oligomeric pores produced using the method and polynucleotide characterisation using the hetero-oligomeric pores.
    Type: Application
    Filed: February 17, 2016
    Publication date: April 5, 2018
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, John Joseph Kilgour, Neil Roger Wood
  • Enzyme-pore constructs
    Patent number: 9885078
    Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
    Type: Grant
    Filed: August 8, 2014
    Date of Patent: February 6, 2018
    Assignee: Oxford Nanopore Technologies Limited
    Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
  • HETERO-PORES
    Publication number: 20180030526
    Abstract: The invention relates to hetero-oligomeric pores derived from Msp. The invention also relates to polynucleotide characterisation using the hetero-oligomeric pores.
    Type: Application
    Filed: February 17, 2016
    Publication date: February 1, 2018
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Clive Gavin Brown, James Anthony Clarke, Richard George Hambley, Andrew John Heron, Lakmal Jayasinghe, John Milton, Jonathan Bankes Pugh, Elizabeth Jayne Wallace
  • MUTANT PORES
    Publication number: 20170356037
    Abstract: The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterisation using Msp.
    Type: Application
    Filed: May 1, 2015
    Publication date: December 14, 2017
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • ENZYME-PORE CONSTRUCTS
    Publication number: 20170335384
    Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
    Type: Application
    Filed: August 3, 2017
    Publication date: November 23, 2017
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
  • MUTANT PORES
    Publication number: 20170306398
    Abstract: The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterisation using Msp.
    Type: Application
    Filed: October 6, 2015
    Publication date: October 26, 2017
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Andrew John Heron, James White
  • Enzyme construct
    Patent number: 9797009
    Abstract: The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
    Type: Grant
    Filed: July 18, 2013
    Date of Patent: October 24, 2017
    Assignee: Oxford Nanopore Technologies Limited
    Inventors: Andrew Heron, James Clarke, Ruth Moysey, Elizabeth Jayne Wallace, Mark Bruce, Lakmal Jayasinghe, Domenico Caprotti, Szabolcs Soeroes, Luke McNeill, Mihaela Misca
  • MUTANT CSGG PORES
    Publication number: 20170283470
    Abstract: The invention relates to mutant forms of the outer membrane located lipoprotein CsgG, in particular, modifications at one or more of positions Tyr51; Asn55; and Phe56. The invention also relates to analyte detection and characterisation using said mutant CsgG.
    Type: Application
    Filed: September 1, 2015
    Publication date: October 5, 2017
    Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • Mutant lysenin pores
    Patent number: 9777049
    Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterization using lysenin.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: October 3, 2017
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Mark Bruce, James Clarke, Andrew Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • METHOD FOR NANOPORE RNA CHARACTERISATION
    Publication number: 20170253923
    Abstract: The invention relates to a new method of characterising FIG. 3 a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.
    Type: Application
    Filed: October 19, 2015
    Publication date: September 7, 2017
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White
  • Mutant pores
    Patent number: 9751915
    Abstract: The invention relates to mutant forms of Msp. The invention also relates to nucleic acid characterization using Msp.
    Type: Grant
    Filed: February 10, 2012
    Date of Patent: September 5, 2017
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: James Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James White