Patents by Inventor Lakmal Jayasinghe

Lakmal Jayasinghe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHOD OF IMPROVING THE MOVEMENT OF A TARGET POLYNUCLEOTIDE WITH RESPECT TO A TRANSMEMBRANE PORE
    Publication number: 20200224262
    Abstract: The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein.
    Type: Application
    Filed: September 17, 2019
    Publication date: July 16, 2020
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Clive Gavin Brown, James White, Andrew John Heron, Mark John Bruce, Christopher Peter Youd, Rebecca Victoria Bowen
  • METHOD FOR NANOPORE RNA CHARACTERISATION
    Publication number: 20200063199
    Abstract: The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.
    Type: Application
    Filed: September 19, 2019
    Publication date: February 27, 2020
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White
  • MUTANT PORES
    Publication number: 20200017556
    Abstract: The invention relates to mutant forms of CsgG. The invention also related to analyte detection and characterisation using CsgG.
    Type: Application
    Filed: July 25, 2019
    Publication date: January 16, 2020
    Applicants: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • MODIFIED NANOPORES, COMPOSITIONS COMPRISING THE SAME, AND USES THEREOF
    Publication number: 20200010511
    Abstract: Provided herein relate to modified or mutant forms of secretin and compositions comprising the same. In particular, the modified or mutant forms of secretin permits efficient capture and/or translocation of an analyte through the modified or mutant secretin nanopores. Methods for using unmodified secretin or the modified or mutant forms of secretin and compositions, for example, for characterizing an analyte, e.g., a target polynucleotide, are also provided.
    Type: Application
    Filed: February 12, 2018
    Publication date: January 9, 2020
    Applicant: Oxford Nanopore Technologies Limited
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh
  • Method for nanopore RNA characterisation
    Patent number: 10480026
    Abstract: The invention relates to a new method of characterizing a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.
    Type: Grant
    Filed: October 19, 2015
    Date of Patent: November 19, 2019
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Daniel Ryan Garalde, Andrew John Heron, Lakmal Jayasinghe, Daniel John Turner, James White
  • METHOD FOR CONTROLLING THE MOVEMENT OF A POLYNUCLEOTIDE THROUGH A TRANSMEMBRANE PORE
    Publication number: 20190345550
    Abstract: The invention relates to new methods of controlling the movement of polynucleotides through transmembrane pores. The invention also relates to new methods of characterising target polynucleotides using helicases.
    Type: Application
    Filed: July 9, 2019
    Publication date: November 14, 2019
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Rebecca Victoria Bowen, Clive Gavin Brown, Mark John Bruce, Andrew John Heron, Elizabeth Jayne Wallace, James White, Joseph Hargreaves Lloyd, David Antoni Alves, Domenico Caprotti, Lakmal Jayasinghe, Luke McNeill, John Milton, Antonino Puglisi, Szabolcs Soeroes
  • Hetero-pores
    Patent number: 10472673
    Abstract: The invention relates to hetero-oligomeric pores derived from Msp. The invention also relates to polynucleotide characterisation using the hetero-oligomeric pores.
    Type: Grant
    Filed: February 17, 2016
    Date of Patent: November 12, 2019
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Clive Gavin Brown, James Anthony Clarke, Richard George Hambley, Andrew John Heron, Lakmal Jayasinghe, John Milton, Jonathan Bankes Pugh, Elizabeth Jayne Wallace, James White
  • MUTANT PORE
    Publication number: 20190330282
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.
    Type: Application
    Filed: March 2, 2017
    Publication date: October 31, 2019
    Applicant: Oxford Nanopore Technologies Limited
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
    Patent number: 10443097
    Abstract: The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein. The invention also relates to improved transmembrane pores and polynucleotide binding proteins.
    Type: Grant
    Filed: May 1, 2015
    Date of Patent: October 15, 2019
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Clive Gavin Brown, James White, Andrew John Heron, Mark John Bruce, Christopher Peter Youd, Rebecca Victoria Bowen
  • MUTANT PORES
    Publication number: 20190300582
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.
    Type: Application
    Filed: March 2, 2017
    Publication date: October 3, 2019
    Applicant: Oxford Nanpore Technolgies Limited
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Mutant CsgG pores
    Patent number: 10400014
    Abstract: The invention relates to mutant forms of the outer membrane-located lipoprotein CsgG, in particular, modifications at one or more of positions Tyr51; Asn55; and Phe56. The invention also relates to analyte detection and characterisation using said mutant CsgG.
    Type: Grant
    Filed: September 1, 2015
    Date of Patent: September 3, 2019
    Assignees: Oxford Nanopore Technologies Ltd., VIB VZW, Vrije Universiteit Brussel
    Inventors: Stefan Howorka, Han Remaut, Lakmal Jayasinghe, Elizabeth Jayne Wallace, James Anthony Clarke, Richard George Hambley, Jonathan Bankes Pugh
  • Method for controlling the movement of a polynucleotide through a transmembrane pore
    Patent number: 10392658
    Abstract: The invention relates to new methods of controlling the movement of polynucleotides through transmembrane pores. The invention also relates to new methods of characterizing target polynucleotides using helicases.
    Type: Grant
    Filed: September 10, 2014
    Date of Patent: August 27, 2019
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Rebecca Victoria Bowen, Clive Gavin Brown, Mark Bruce, Andrew John Heron, Elizabeth Jayne Wallace, James White, Joseph Hargreaves Lloyd, David Antoni Alves, Domenico Caprotti, Lakmal Jayasinghe, Luke McNeill, John Milton, Antonino Puglisi, Szabolcs Soeroes
  • MUTANT PORE
    Publication number: 20190202876
    Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.
    Type: Application
    Filed: April 6, 2017
    Publication date: July 4, 2019
    Applicant: Oxford Nanopore Technologies Limited
    Inventors: Lakmal Jayasinghe, Mark John Bruce, Luke McNeill, Ramiz Iqbal Nathani, Pratik Raj Singh, Neil Roger Wood, Stephen Robert Young
  • Mutant pores
    Patent number: 10266885
    Abstract: The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterization using Msp.
    Type: Grant
    Filed: October 6, 2015
    Date of Patent: April 23, 2019
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Andrew John Heron, James White
  • MUTANT PORE
    Publication number: 20190071721
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.
    Type: Application
    Filed: March 2, 2017
    Publication date: March 7, 2019
    Applicant: Oxford Nanopore Technologies Limited
    Inventors: Lakmal JAYASINGHE, Elizabeth Jayne WALLACE
  • ENZYME-PORE CONSTRUCTS
    Publication number: 20190002972
    Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
    Type: Application
    Filed: September 12, 2018
    Publication date: January 3, 2019
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
  • Mutant pores
    Patent number: 10167503
    Abstract: The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterization using Msp.
    Type: Grant
    Filed: May 1, 2015
    Date of Patent: January 1, 2019
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Enzyme-pore constructs
    Patent number: 10077471
    Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.
    Type: Grant
    Filed: September 18, 2015
    Date of Patent: September 18, 2018
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Lakmal Jayasinghe, John Hagan Pryce Bayley, Stephen Cheley, Brian McKeown, James White, James Anthony Clarke
  • ENZYME CONSTRUCT
    Publication number: 20180230526
    Abstract: The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
    Type: Application
    Filed: September 14, 2017
    Publication date: August 16, 2018
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Andrew John Heron, James Anthony Clarke, Ruth Moysey, Elizabeth Jayne Wallace, Mark Bruce, Lakmal Jayasinghe, Domenico Caprotti, Szabolcs Soeroes, Luke McNeill, Mihaela Misca
  • MUTANT LYSENIN PORES
    Publication number: 20180208632
    Abstract: The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.
    Type: Application
    Filed: August 31, 2017
    Publication date: July 26, 2018
    Applicant: Oxford Nanopore Technologies Ltd.
    Inventors: Mark Bruce, James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace