Abstract: A nanoparticle drug based on a small interfering RNA (siRNA) targeting JFK and use thereof are provided. Nucleotide sequences of the sense strand and the antisense strand of the siRNA targeting JFK are set forth in SEQ ID NO: 1 and SEQ ID NO: 2, respectively. Liposomes can be used to encapsulate the complex of the siRNA and a protamine to produce a nanoparticle drug targeting JFK, which may be used to treat a disease related to abnormally-high expression of JFK. In this disclosure, breast cancer 4T1 cell-based tumor-bearing mice serve as the model for evaluating the anti-tumor activity of combined therapy involving the nanoparticle drug targeting JFK and radiotherapy. The results reveal that this combined therapy exhibits superior anti-tumor activity compared to the control group.

Abstract: Provided are a rolling circle amplification method, a method for preparing a sequencing library, and a DNA nanoball prepared therefrom. The rolling circle amplification method includes: sequentially denaturing and annealing a double-stranded DNA and a mediating sequence in a same system, to complementarily pair the mediating sequence with two ends of a denatured single-stranded DNA; simultaneously introducing a ligase and a polymerase into the system to connect the two ends of the single-stranded DNA under action of the ligase; and performing a rolling circle amplification reaction under action of the polymerase by using the mediating sequence as a primer and the single-stranded DNA as a template, to obtain DNA nanoball.

Abstract: Disclosed are a floating tray mechanism and a robot. The floating tray mechanism includes a tray and a mounting bracket, the mounting bracket support the tray; the floating tray mechanism further comprises a floating contacting portion and a pulling rebounding portion, the floating contacting portion is provided on one of the tray and the mounting bracket, and the tray is movably supported on the mounting bracket, the pulling rebounding portion is configured to be able to pull the tray back to an initial position of translation when the tray moves relative to the mounting bracket. In the present application, the tray will have a damping relative stroke and a recovery relative stroke relative to the robot when a robot suddenly stops or suddenly speeds up, which greatly reduces the inertial motion of the goods, and thus the fluid goods will not spill out of the tray.

Abstract: Provided are a rolling circle amplification method, a method for preparing a sequencing library, and a DNA nanoball prepared therefrom. The rolling circle amplification method includes: sequentially denaturing and annealing a double-stranded DNA and a mediating sequence in a same system, to complementarily pair the mediating sequence with two ends of a denatured single-stranded DNA; simultaneously introducing a ligase and a polymerase into the system to connect the two ends of the single-stranded DNA under action of the ligase; and performing a rolling circle amplification reaction under action of the polymerase by using the mediating sequence as a primer and the single-stranded DNA as a template, to obtain DNA nanoball.

Abstract: An in-situ large-area drilling and furnace construction method for coal gasification and co-mining of kerosene and/or coalbed methane is provided, which includes: constructing an injection well to the target coal seam and then constructing a horizontal well to divide the target coal seam into sections of coal; extracting coalbed methane; transforming the horizontal wells on both sides of the coal in the first section of coal into coal gasification production wells, and dividing them into intake and exhaust channels; constructing ventilation boreholes to form a “honeycomb shaped” gasifier, which ignites and gasifies to promote the production of combined gases from combustion; after the combustion of first section of coal is completed, continuing to construct the next section of coal and drill holes between adjacent horizontal wells to achieve continuous coal seam combustion and gasification. The method of the present disclosure can improve combustion efficiency and increase kerosene extraction efficiency.

Abstract: Provided is a method for sequencing a long-fragment nucleic acid. The nucleic acid molecules each containing a long insert, a first sequencing adapter, and a second sequencing adapter, is used to construct a sequencing library, and the sequencing is performed in segments to sequence the nucleic acids having the long inserts.

Abstract: The present application discloses a cell slitting method and a slitting device. The cell slitting method includes: controlling a pressing plate to tightly press a lamination unit on a cell support table, and controlling the slitting device to slit multiple layers of membranes of the lamination unit according to a pre-set slitting line to obtain m×n independent cell bodies. With respect to the existing slitting device using the mode of cutting off a single cell single-layer tail membrane, according to the present application, the slitting device slits the lamination unit according to a pre-set slitting line such that m×n independent cell bodies can be obtained by simultaneously cutting off multiple layers of membranes, which greatly improves the slitting efficiency and improves the production efficiency of cells.

Abstract: The present application discloses a method and device for stacking cell pole pieces. The first mechanical hand simultaneously grabs corrected m×n first pole pieces from the first position correction platform, and simultaneously places the m×n first pole pieces on a separator of the same lamination table in m rows and n columns for lamination, and the second mechanical hand simultaneously grabs corrected mxn second pole pieces from the second position correction platform and simultaneously places m×n second pole pieces on a separator on the same lamination table in m rows and n columns for lamination. By simultaneously laminating m×n pole pieces on the same lamination table in an arrangement of m rows and n columns, multiple pole pieces can be stacked at one time, so as to improve the lamination efficiency.

Abstract: In a method for playing a first game video, a video playing interface is displayed in a game application. Preview information of at least one game video of a game video library is displayed. The preview information includes description information that is determined based on an account tag of a user account and content of the first game video from the at least one game video. The account tag is determined based on game play activity information of the user account associated with the game application. Based on a user operation performed on the first game video from the at least one game video, the first game video is played in the video playing interface. Apparatus and non-transitory computer-readable storage medium counterpart embodiments are also contemplated.

Abstract: A target tracking method and device, relating to the field of vehicles. The method comprises: reading a target fusion list at the current moment, wherein the target fusion list comprises a target attribute set corresponding to each environment target in one or more environment targets, and the target attribute set comprises parameter attribute sets, measured by one or more sensors, of the environment targets; determining whether the target fusion list at the current moment is an initial target fusion list or not; and if the target fusion list at the current moment is not the initial target fusion list, determining a target tracking list at the current moment according to the target fusion list at the current moment and a stored target tracking list at the previous moment, wherein the target tracking list comprises a parameter attribute set corresponding to each screened environment target and a fusion tracking ID.

Abstract: The present disclosure provides the utilization of an RPN11 marker in detecting myeloma and assessing disease risk, prognostic analysis, and therapeutic drug development. This includes a detection reagent and a kit for detecting smoldering multiple myeloma (SMM) and multiple myeloma (MM) and assessing disease risk. Additionally, it involves molecular typing of MM patients' cassette, prognostic analysis of the MM patients, and predicting the response of the MM patients to a bortezomib treatment. It entails the use of an RPN11 inhibitor in preparation of a pharmaceutical composition for treating MM characterized by drug-resistant t(4;14) translocation and a high RPN11 expression level. Notably, the combined use of the RPN11 with histone methyltransferase (HMT) enhances detection sensitivity and accuracy, preventing a mismatch between the patients' genotype and the prediction of disease development, and can effectively predict the overall survival and prognosis of MM patients with t(4;14) translocation.

Abstract: A nanoparticle drug based on a small interfering RNA (siRNA) targeting JFK and use thereof are provided. Nucleotide sequences of the sense strand and the antisense strand of the siRNA targeting JFK are set forth in SEQ ID NO: 1 and SEQ ID NO: 2, respectively. Liposomes can be used to encapsulate the complex of the siRNA and a protamine to produce a nanoparticle drug targeting JFK, which may be used to treat a disease related to abnormally-high expression of JFK. In this disclosure, breast cancer 4T1 cell-based tumor-bearing mice serve as the model for evaluating the anti-tumor activity of combined therapy involving the nanoparticle drug targeting JFK and radiotherapy. The results reveal that this combined therapy exhibits superior anti-tumor activity compared to the control group.

Abstract: A use of a detection reagent for a microRNA (miRNA) marker miR-143-3p in the preparation of a product for evaluating a therapeutic effect of olanzapine in the treatment of schizophrenia (SZ) and a kit are provided. An expression level of miR-143-3p can be detected to determine a therapeutic effect of olanzapine in the treatment of SZ of a patient, and can be used as a detection or treatment index in clinical practice, which provides a theoretical basis for the mechanism research of SZ and the clinical application.

Abstract: Disclosed are a road feature point extraction method and system. The road feature point extraction method comprises: acquiring map information for the current position of a vehicle, wherein the map information comprises the attributes of the current road and the attributes of the next road section; comparing the attributes of the current road with the attributes of the next road section to determine the road type of the next road section; and extracting, in conjunction with the road type of the next road section and the map information corresponding to the next road section, a road feature point representing a road scene on the next road section, and outputting the name of the extracted road feature point and information of the relative distance between the road feature point and the vehicle.

Abstract: Dual nucleic acid and protein isoform measurements are performed on low starting cell numbers (e.g. equivalent to the number of blastomeres composing early embryonic development stages (morula and blastocysts)), comprising integrating fractionation polyacrylamide gel electrophoresis (fPAGE) of 10-100 cells with off-chip analysis of nucleic acids in the nuclei.

Abstract: A traffic lane line fitting method includes: obtaining map information for a current position of a vehicle, the map information comprising a number of traffic lanes, a road width and line point information of traffic lane lines on both sides of the vehicle; determining, from among the traffic lane lines on both sides of the vehicle, a traffic lane line on one side, consistent with the direction indicated by at least one of the following, to be a traffic lane line offset reference used for traffic lane line fitting; offsetting and generating a plurality of traffic lane lines of the vehicle on the basis of the traffic lane line offset reference; performing curve fitting on a set of line points on the generated plurality of traffic lane lines so as to obtain a corresponding traffic lane line equation.

Abstract: Disclosed are a floating tray mechanism and a robot. The floating tray mechanism includes a tray and a mounting bracket, the mounting bracket support the tray; the floating tray mechanism further comprises a floating contacting portion and a pulling rebounding portion, the floating contacting portion is provided on one of the tray and the mounting bracket, and the tray is movably supported on the mounting bracket, the pulling rebounding portion is configured to be able to pull the tray back to an initial position of translation when the tray moves relative to the mounting bracket. In the present application, the tray will have a damping relative stroke and a recovery relative stroke relative to the robot when a robot suddenly stops or suddenly speeds up, which greatly reduces the inertial motion of the goods, and thus the fluid goods will not spill out of the tray.

Abstract: A method for controlling lane changing of a vehicle, including: receiving a lane changing instruction (step 101); acquiring a speed parameter of the vehicle (step 102); according to the speed parameter, determining a controlling coefficient of a predetermined controller (step 103); acquiring a position-deviation parameter and a heading-angle parameter of the vehicle; if the position-deviation parameter is greater than or equal to a first threshold, or, if the heading-angle parameter is greater than or equal to a second threshold, according to the controlling coefficient, the position-deviation parameter and the heading-angle parameter, determining a target steering-wheel steering angle of the vehicle; and according to the target steering-wheel steering angle, controlling the vehicle to perform a lane changing operation, till the position-deviation parameter is less than the first threshold, and the heading-angle parameter is less than the second threshold (step 105).