Abstract: The invention provides a method for preparing an expanded cell or tissue sample suitable for microscopic analysis. Expanding the sample can be achieved by binding, e.g., anchoring, key biomolecules to a DMAA-TF polymer network and swelling, or expanding, the polymer network, thereby moving the biomolecules apart as further described herein. As the biomolecules are anchored to the polymer network isotropic expansion of the polymer network retains the spatial orientation of the biomolecules resulting in an expanded, or enlarged, sample.

Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA. Aspects of the invention in particular relate to Cpf1 mutants having altered PAM specificity.

Abstract: Engineered systems comprising components of defense systems identified in prokaryotes are provided.

Abstract: A profile download method and system, and a related device are provided, and the method includes: obtaining, by a user terminal, local profile assistant LPA indication information, and obtaining download information required for downloading a target profile; and sending, by the user terminal, the download information to an embedded universal integrated circuit card eUICC if the LPA indication information instructs to download the profile by using an LPA in the eUICC, so that the eUICC downloads, by using the LPA in the eUICC, the target profile from a download server indicated by the download information. According to the method, the profile can be downloaded when an LPA is deployed in both the user terminal and the eUICC.

Abstract: An embedded universal integrated circuit card (eUICC) profile management method and apparatus update an operating system of an eUICC include setting, by a local profile assistant (LPA), an operating system update flag, and obtaining and storing first metadata of a first profile. A profile server generates a second profile based on the operating system update flag, and generates second metadata of the second profile. The LPA configures the second metadata based on the first metadata, and activates the second profile based on the second metadata.

Abstract: Novel programmable targeting sequences and applications thereof. The targeting sequences can be engineered for binding to proteins, polypeptides, and other macromolecules.

Abstract: Disclosed and claimed are mutation(s) or modification(s) of the CRISPR enzyme, for example a Cas enzyme such as a Cas9, which obtain an improvement, for instance a reduction, as to off-target effects of a CRISPR-Cas or CRISPR-enzyme or CRISPR-Cas9 system or complex containing or including such a mutated or modified Cas or CRISPR enzyme or Cas9. Methods for making and using and uses of such mutated or modified Cas or CRISPR enzyme or Cas9 and systems or complexes containing the same and products from such methods and uses are also disclosed and claimed.

Abstract: Embodiments of the present invention disclose a method for adding an authentication algorithm program, and a relevant device and system, where the method includes: receiving, by an SM-DP+ server, an authentication algorithm program sent by an MNO, where the authentication algorithm program corresponds to target information, and the target information is at least one of: firmware version information of an eUICC, an EID issuer identifier of the eUICC, platform/operating system version information of the eUICC, or capability information of the eUICC; and generating, by the SM-DP+ server, a bound profile package that includes the authentication algorithm program, and sending the bound profile package to the eUICC by using an LPA. As can be learned, the eUICC can add the authentication algorithm program into the eUICC in time by implementing the authentication algorithm program described in a first aspect.

Abstract: An embedded universal integrated circuit cart (eUICC) profile management method and apparatus to update an operating system of an eUICC include setting, by a local profile assistant (LPA), an operating system update flag, and obtaining and storing first metadata of a first profile. A profile server generates a second profile based on the operating system update flag, and generates second metadata of the second profile. The LPA configures the second metadata based on the first metadata, and activates the second profile based on the second metadata.

Abstract: An information verification method and a related device. The method includes: receiving, by a first profile server, a remote profile management request sent by a remote profile management server, where the remote profile management request includes a first profile owner identifier; obtaining a second profile owner identifier in profile information; determining whether the first profile owner identifier is the same as the second profile owner identifier; and sending a remote profile management command to user equipment when the first profile owner identifier is the same as the second profile owner identifier. In this way, a loss caused to an operator or a profile owner due to a user's access to an invalid network or random profile unlocking is avoided, network security is improved, and waste of network resources is reduced.

Abstract: An application processing method and apparatus for an embedded universal integrated circuit card (eUICC) are disclosed. The method includes: activating, by a terminal device, an embedded universal integrated circuit card (eUICC); and selecting, by the terminal device, an Issuer Security Domain Root (ISD-R) application on the eUICC during an initialization process of the eUICC, to maintain a session between the terminal device and the eUICC. The session between the terminal device and the eUICC can be maintained during the initialization process of the eUICC.

Abstract: A method for downloading a profile of an operator includes sending, by a terminal to a subscription manager-data preparer (SMDP) using a subscription manager-secure router (SM-SR), a request for downloading a profile of an operator, where the download request carries the download certificate, an identifier (ID) of an embedded universal integrated circuit card (eUICC) of the terminal, and addressing information of the SM-DP; and receiving, by the terminal, the profile of the operator that is sent by the SM-DP using the SM-SR and that corresponds to the download request, and transmitting the profile of the operator to the eUICC, where the profile of the operator is obtained by the SM-DP according to an identity of the profile of the operator after the SM-DP verifies that the certificate that is for downloading the profile of the operator and is carried in the download request is valid.

Abstract: Embodiments of this application provide a method for updating network access application authentication information. The method is applicable to a terminal. The terminal has an eUICC, and the eUICC has at least one profile. The method includes: receiving, by the terminal, a first message from a remote server, where the first message includes network access application NAA authentication parameter update information and a first identifier; updating, by the terminal, authentication information of a profile corresponding to the first identifier based on the NAA authentication parameter update information; and detaching, by the terminal, from a network, and re-attaching to the network based on updated authentication information of the profile. According to the embodiments of this application, the NAA authentication information can be updated, and communication security can be improved by periodically or aperiodically updating the NAA authentication information.

Abstract: A profile download method and system, and a related device are provided, and the method includes: obtaining, by a user terminal, local profile assistant LPA indication information, and obtaining download information required for downloading a target profile; and sending, by the user terminal, the download information to an embedded universal integrated circuit card eUICC if the LPA indication information instructs to download the profile by using an LPA in the eUICC, so that the eUICC downloads, by using the LPA in the eUICC, the target profile from a download server indicated by the download information. According to the method, the profile can be downloaded when an LPA is deployed in both the user terminal and the eUICC.

Abstract: Embodiments include a method for installing a subscription profile, a terminal, and a server. The method includes obtaining, by a local profile assistant (LPA) in the terminal, metadata of a subscription profile, determining, based on an authorization file, whether use of each policy rule included in the metadata is allowed. The method further includes obtaining, by the LPA, the subscription profile when the use of each policy rule included in the metadata is allowed. The method further includes sending the subscription profile to an embedded universal integrated circuit card (eUICC) in the terminal. The method further includes determining, by the eUICC based on the authorization file, whether use of the subscription profile is allowed. The method further includes installing, by the eUICC, the subscription profile when the use of the subscription profile is allowed.

Abstract: A method for authorizing management for an embedded universal integrated circuit card includes: generating, by an eUICC manager, authorization information (S101); encrypting the authorization information by using eUICC management credential (S102); and sending the encrypted authorization information to an eUICC (S103), where the authorization information includes an identifier of at least one first device; or the authorization information includes at least one authorization credential. The authorization information is configured in the eUICC, and therefore, when a subsequently authorized first device manages a profile in the eUICC, the eUICC may directly accept or reject, according to a stored correspondence between a profile management function and an authorized first device, to be managed, without obtaining authorization information each time.

Abstract: A profile download method and system, and a related device, where the method includes obtaining, by a user terminal, local profile assistant (LPA) indication information, obtaining download information required for downloading a target profile, and sending, by the user terminal, the download information to an embedded universal integrated circuit card (eUICC) when the LPA indication information instructs to download the profile using an LPA in the eUICC such that the eUICC downloads, using the LPA in the eUICC, the target profile from a download server indicated by the download information. Hence, the profile can be downloaded when an LPA is deployed in both the user terminal and the eUICC.

Abstract: Disclosed and claimed are mutation(s) or modification(s) of the CRISPR enzyme, for example a Cas enzyme such as a Cas9, which obtain an improvement, for instance a reduction, as to off-target effects of a CRISPR-Cas or CRISPR-enzyme or CRISPR-Cas9 system or complex containing or including such a mutated or modified Cas or CRISPR enzyme or Cas9. Methods for making and using and uses of such mutated or modified Cas or CRISPR enzyme or Cas9 and systems or complexes containing the same and products from such methods and uses are also disclosed and claimed.

Abstract: Embodiments of the present invention disclose a method for adding an authentication algorithm program, and a relevant device and system, where the method includes: receiving, by an SM-DP+ server, an authentication algorithm program sent by an MNO, where the authentication algorithm program corresponds to target information, and the target information is at least one of: firmware version information of an eUICC, an EID issuer identifier of the eUICC, platform/operating system version information of the eUICC, or capability information of the eUICC; and generating, by the SM-DP+ server, a bound profile package that includes the authentication algorithm program, and sending the bound profile package to the eUICC by using an LPA. As can be learned, the eUICC can add the authentication algorithm program into the eUICC in time by implementing the authentication algorithm program described in a first aspect.

Abstract: Embodiments of the present invention disclose a method and system for updating a certificate issuer public key, and a related device. The method includes: receiving, by an embedded universal integrated circuit card eUICC, first information sent by a local profile assistant LPA, where the first information includes a first certificate issuer CI public key identifier, and the first CI public key identifier is a CI public key identifier that the eUICC does not have; sending, by the eUICC, second information to an OPS by using the LPA, where the second information includes the first CI public key identifier; receiving, by the eUICC, a patch package sent by the OPS by using the LPA, where the patch package includes at least a first CI public key corresponding to the first CI public key identifier; and updating, by the eUICC, a CI public key of the eUICC by using the first CI public key.