Abstract: The present disclosure relates to improved biomanufacturing systems and methods with an ability to effectively control process variability to consistently generate high-quality cell-based therapies. In particular, disclosed herein is a using model-based control for determining process parameters in a bioreactor in real time. Additionally disclosed herein is a system for generating an AI digital twin model for a commercial-scale bioreactor.

Abstract: Techniques for controlling a trajectory of a mud motor directional drill are disclosed. A system receives one or more inputs pertaining to constraints for a wellbore in a mud motor drilling operation. The system constructs, as a function of the one or more inputs, a discretized well depth horizon. One or more binary slide and rotate commands for controlling a directional drill within the wellbore is generated based on an analysis of the discretized well depth horizon. The system controls the directional drill according to the one or more binary slide and rotate commands.

Abstract: Provided herein are compositions, systems, and methods comprising engineered effector proteins and uses thereof. These effector proteins may be characterized as CRISPR-associated (Cas) proteins. Various compositions, systems, and methods of the present disclosure may leverage the activities of these effector proteins for the modification, detection, and engineering of nucleic acids.

Abstract: A self-stabilizing integrally-molded pillar filling structure for underground mines is provided, belonging to the field of supporting structures, which includes an airbag, and the airbag is provided with a feed filling port and a gas inflation port; a telescopic bearing piece, and the telescopic bearing piece is fixedly provided on an inner surface of the airbag and is capable of expanding and contracting with volume change of an inner cavity of the airbag; and steel hoops, and the steel hoops are fixedly provided on an outer surface of the airbag.

Abstract: The present disclosure provides to systems, methods, and compositions for template-based DNA, synthesis. Particularly the present disclosure provides systems, methods, and compositions for generating multi-site (e.g., three or more) sequence variants of template nucleic acid strands kilobases (e.g., greater than Ikb) m length.

Abstract: A prefabricated tunnel prestressed anchor comprises an anchor rod, the anchor rod comprises a free section and a prestressed section which are arranged in sequence; the prestressed section of the anchor rod is sleeved with a steel casing, holes are formed in the steel casing, a filler material is arranged between the steel casing and the anchor rod and filled in a space between the steel casing and the anchor rod after a prestress is applied to the anchor rod on a tensioning bench, and when the filler material is electrified, the structure of the filler material is destroyed; sleeves are arranged at two ends of the steel casing, and grout plugs used for sealing the steel casing are arranged in openings of the sleeves. The prestressed anchor can be directly installed in site to be used, a prestress is applied after the structure of the filler material is destroyed.

Abstract: A precombustion chamber igniter with a retractable electric glow plug and a cold start control method for a methanol engine are provided. The precombustion chamber igniter includes a fuel injector, a spark plug, and an electric glow plug. The electric glow plug is retractable and has two working positions, at a first position, a heating segment is wholly located in an injection chamber to provide heat energy for the injection chamber, and at a second position, the heating segment is partially located in the injection chamber and passes through a first through hole, and the rest of the heating segment is located in a main combustion chamber of an engine, to provide heat energy for the injection chamber and the main combustion chamber. In a cold start stage of the engine, the heating segment of the electric glow plug is controlled to be located at the second position.

Abstract: An ammonia-rich combustion coupled dual-mode prechamber ammonia-fueled engine control system is provided, including an ammonia-fueled engine and an electronic control unit. The ammonia-fueled engine includes a combustion chamber, a dual-mode jet ignition device, and an ammonia injector. The dual-mode jet ignition device includes a prechamber, an air injector, and a fuel injector. When the engine operates, ammonia fuel is firstly injected into the combustion chamber by the ammonia injector to form a rich ammonia-air mixture with air in the combustion chamber. During a compression stroke of the engine, the rich ammonia-air mixture in the combustion chamber is entered into the prechamber through a jet hole. The air injector performs scavenging in the prechamber until a equivalence ratio of prechamber gas mixture is less than 1.0, and then the fuel injector injects fuel into the prechamber forming target mixture. Finally, the target mixture is ignited by the spark plug.

Abstract: A portable standby starting device for a vehicle includes a battery circuit, a load access detecting circuit, and a vehicle starting circuit. The battery circuit is coupled to the load access detecting circuit and the vehicle starting circuit, and is configured to supply power to the load access detecting circuit and the vehicle starting circuit; the load access detecting circuit is coupled to the vehicle starting circuit, and is configured to detect whether the vehicle starting circuit is connected to a vehicle load; the vehicle starting circuit is configured to, when the load access detecting circuit detects the connection of the vehicle load, output a vehicle starting current for controlling an ignition operation performed for the vehicle.

Abstract: The present disclosure provides methods for conducting in situ multiplex and multi-omics detection and identification using coded padlocks probes. The methods comprise simultaneous use of RNA-specific padlock probes and polypeptide-specific padlock probes to detect both RNA and polypeptides in a cellular sample. Both types of probes include a barcode that unique identifies the RNA or polypeptide that that padlock probe detects. Both types of probes also include a batch-specific sequencing primer binding site to enable sequencing a desired subset of concatemer template molecules. Use of the batch-specific sequencing primers reduces overcrowding signals and images, to produces optical images that are intense and resolvable. By conducting multiple rounds of sequencing on the same cellular sample using different batch-specific sequencing primers enables multiplex and multi-omics sequencing to reveal numerous target RNAs and their encoded polypeptides.

Abstract: A method for synthesizing diacylglycerol, comprising the following steps: (1) mixing an oil, a lipase and a solvent, and carrying out a hydrolysis reaction to obtain a hydrolyzate; and (2) under the action of a diacylglycerol lipase, performing an esterification reaction on the hydrolyzate obtained in step (1) with glycerol; the lipase in step (1) is lipase MAS1 or MAS1-H108A.

Abstract: Among the various aspects of the present disclosure is the provision of methods of diagnosing and treating inflammatory bowel disease (IBD) including ulcerative colitis (UC) or Crohn's disease (CD). In particular, the present disclosure provides in part a panel of IBD biomarkers useful in diagnosing and making treatment decisions. In addition, the present disclosure provides methods of treating IBD with a plasminogen activator inhibitor-1 (PAI-1) inhibitor or tissue plasminogen activator (tPA).

Abstract: Embodiments of the present disclosure relate to nucleotide molecules with a 3? AOM blocking group. Also provided herein are methods to prepare such nucleotide molecules, and the uses of fully functionalized nucleotides containing the 3?-OH blocking group for sequencing applications.

Abstract: An eyepiece waveguide for augmented reality applications includes a substrate and a set of incoupling diffractive optical elements coupled to the substrate. A first subset of the set of incoupling diffractive optical elements is operable to diffract light into the substrate along a first range of propagation angles and a second subset of the set of incoupling diffractive optical elements is operable to diffract light into the substrate along a second range of propagation angles. The eyepiece waveguide also includes a combined pupil expander diffractive optical element coupled to the substrate.

Abstract: Provided herein are anaplastic lymphoma kinase (ALK) fusion nucleic acid molecules and polypeptides, methods related to detecting ALK fusion nucleic acid molecules and polypeptides in cancer, as well as methods of treatment and uses related thereto. Detection of an ALK fusion nucleic acid molecule or polypeptide can be used to identify individuals that may benefit from treatment with an anti-cancer therapy.

Abstract: The embodiments of the present disclosure provide a light-emitting substrate and a manufacturing method thereof, a backlight module, and a display device, and the light-emitting substrate includes a base substrate, a light-emitting device and a reflective layer on the base substrate. The reflective layer has an opening, and the light-emitting device is in the opening and spaced apart from the reflective layer; the reflective layer includes an edge part close to the light-emitting device and defining the opening, and a body part on a side of the edge part away from the light-emitting device, wherein the edge part has a plurality of reflective edges facing the light-emitting device and defining the opening, and a plurality of reflective included angles formed by the reflective edges interesting with each other, and the reflective included angles include included angles that are not completely identical in shape or angle.

Abstract: An internal circulation type clathrate hydrate-based cold storage system and method are provided in the field of industrial refrigeration. The internal circulation type clathrate hydrate-based cold storage system includes a water chilling unit, a hydrate-based cold storage tank, a gas disturbance device, a concentration measurement system, a heat exchange system, a direct-current power supply and circuit control system, and a system monitoring device. The water chilling unit provides a secondary refrigerant at a low temperature. The secondary refrigerant flows through an evaporator coil in the hydrate-based cold storage tank for a heat exchange. The gas disturbance device induces hydrate nucleation. The concentration measurement system monitors a concentration of a hydrate solution in real time. The heat exchange system is equipped with a finned tube heat exchanger with a fan.

Abstract: A method for optimizing computing power of a neural network module, a chip, an electronic device, and a medium are provided. The method includes: obtaining, by the chip, a computational graph of the neural network module having respective operators; performing at least one of adjustments below at least 1 time on a first operator in the computational graph according to specific operation of each operator: counterchanging a position of the first operator with a subsequent operator or a preceding operator in the computational graph, splitting the first operator into more than two identical operators, and inserting a plurality of first operators that are capable of canceling each other out; determining a second operator adjacent to the adjusted first operator in the computational graph according to the specific operations of each operator; and performing merge or cancellation, by the chip, on the adjusted first operator and the second operator.

Abstract: A combination thermal module includes a vapor chamber defining an airtight chamber filled with a working fluid and having at least one through hole formed thereon communicable with the airtight chamber; at least one annular wick structure provided in the airtight chamber corresponding to the through hole; and at least one heat pipe has an open end inserted into the airtight chamber to contact with a first wick structure on an inner lower surface of the vapor chamber and be axially supported on the annular wick structure. The annular wick structure includes communicable axial and radial passages, allowing vaporized working fluid to flow from the vapor chamber to the heat pipe quickly. With these arrangements, the thermal module has a shortened flow-back path between the vapor chamber and the heat pipe to avoid dry burning in the vapor chamber and upgrade the two-phase heat exchange efficiency of the vapor chamber.

Abstract: A load balancing method for two compressors. The two compressors are used in a refrigeration system and are driven coaxially by the same driving device. The method comprises the steps of obtaining parameters, determining balance, and controlling start/stop states. The parameters in the step of obtaining parameters are parameters related to the two compressors, such as a compressor suction side flow rate, or exhaust side flow rate, or suction side temperature; the step of determining balance comprises determining, on the basis of the obtained parameters related to the two compressors, whether load is balanced between the two compressors; the step of controlling start/top states comprises controlling the start/stop states of the two compressors according to whether the load is balanced. The method can monitor the load balance state of two compressors that are coaxially driven, thereby effectively avoiding failure of the refrigeration system caused by unbalanced loads of the compressors.