Abstract: A calorimeter that includes a sample cell, a reference cell, a pressure system that applies a variable pressure to the sample cell, and a pressure controller that controls the pressure applied by the pressure system to the sample sell. By applying identical pressure perturbations to both the sample and reference cells over a range of temperature, the calorimeter can be used to accurately calculate both the thermal coefficient of expansion of various substances, and the volume change of molecules undergoing a structural transition.

Abstract: This invention provides a transgenic mammal containing a defective beta 1 subunit (Kv&bgr;1) of a voltage sensitive potassium channel, where the Kv&bgr;1 subunit is unable to confer N-type inactivation of the K+ but retains the ability to co-associate with Kv1 family &agr;-subunits and thereby enhance channel surface expression. Preferably the Kv&bgr;1.1 gene encoding Kv&bgr;1 subunit has a mutation in all or a portion of codons 1-70 of its inactivation domain. The transgenic mammal is useful as a model for psychiatric and neurological disorders to identify anxiolytic compounds and pro-cognitive functions. The invention also provides for methods for screening and evaluating test compounds for their ability to modulate Kv&bgr;1.1 activity, specifically for inactivation of a potassium channel or for co-association with &agr;-subunits.

Abstract: The present invention provides a conjugate consisting essentially of a NK4 molecule and a polyethylene glycol group having a molecular weight of from about 20 to about 40 kDa. The invention also provides a composition in which the monoPEGylated conjugates comprise at least 90% of the total of pegylated NK4 molecules and unpegylated NK4 molecules in the composition. Also provided is a composition in which the monoPEGylated conjugates comprise conjugates in which the PEG groups are attached to groups randomly selected from the lysine side chains of NK4 molecules and the N-terminal amino groups of NK4 molecules. A method for the treatment of cancer by administering 1 to 30 mg monoPEGylated NK4 per kg per day is further provided.

Abstract: A method of determining a thermal coefficient of expansion of a substance, the method including providing a first liquid holder containing a solution including the substance, and a second liquid holder containing a liquid in which the substance is not present; applying a pressure perturbation to the solution in the first liquid holder and to the liquid in the second liquid holder at a known temperature; determining a differential heat effect between the first and second liquid holders in response to the pressure perturbation; calculating, from the differential heat effect, the heat effect of the substance in response to the pressure perturbation; and determining, from the calculated heat effect, the thermal coefficient of expansion of the substance at the known temperature.

Abstract: A process for selecting human cells for the production of human proteins by endogenous gene activation allows human proteins to be produced in economically feasible quantities and in a form suitable for producing a pharmaceutical composition. Also disclosed is a process for producing human proteins in a cell line identified in this matter.

Abstract: A method according to the invention includes determining a pattern probability for each of a number of target patterns achievable in a bingo-type game. Each pattern probability comprises a probability of achieving the respective target pattern in the bingo-type game. The method next includes associating or mapping different pattern sets to each different prize level in a desired prize distribution. The target patterns and their respective pattern probabilities are assigned or mapped to the different pattern sets so that the individual pattern probabilities included in each pattern set add up to the desired probability of the prize level with which the pattern set is associated.

Abstract: The invention relates to human cells which are capable, on the basis of an activation of the endogenous human EPO gene, of producing EPO in a sufficient amount and purity to make possible a cost-effective production of human EPO as a pharmaceutical preparation. The invention furthermore relates to a method for the preparation of such human EPO-producing cells, DNA constructs for the activation of the endogenous EPO in human cells, and a method for the large technical production of EPO in human cells.

Abstract: A process for selecting human cells for the production of human proteins by endogenous gene activation allows human proteins to be produced in economically feasible quantities and in a form suitable for producing a pharmaceutical composition. Also disclosed is a process for producing human proteins in a cell line identified in this matter.

Abstract: The invention relates to human cells which are capable, on the basis of an activation of the endogenous human EPO gene, of producing EPO in a sufficient amount and purity to make possible a cost-effective production of human EPO as a pharmaceutical preparation. The invention furthermore relates to a method for the preparation of such human EPO-producing cells, DNA constructs for the activation of the endogenous EPO inhuman cells, and a method for the large technical production of EPO in human cells.

Abstract: A calorimeter that includes a sample cell, a reference cell, a pressure system that applies a variable pressure to the sample cell, and a pressure controller that controls the pressure applied by the pressure system to the sample cell. By applying identical pressure perturbations to both the sample and reference cells over a range of temperatures, the calorimeter can be used to accurately calculate both the thermal coefficient of expansion of various substances, and the volume change of molecules undergoing a structural transition.

Abstract: A calorimeter that includes a sample cell, a reference cell, a pressure system that applies a variable pressure to the sample cell, and a pressure controller that controls the pressure applied by the pressure system to the sample cell. By applying identical pressure perturbations to both the sample and reference cells over a range of temperatures, the calorimeter can be used to accurately calculate both the thermal coefficient of expansion of various substances, and the volume change of molecules undergoing a structural transition.

Abstract: A system, method, and computer program product for self-authenticating an end-user of one of multiple service providers, each of the service providers having end-users connected to a common network. A digital repository is populated with information regarding the service providers, the end-users and service description information for the end-users. New devices are detected as they are connected to the high-speed network, and are allocated a limited amount of bandwidth. The end-user is given access to an authentication application. Based on the information entered by the end-user, the database is queried to determine a level of service purchased by the end-user from their service provider. The end-user is then allocated the appropriate level of service.

Abstract: The invention concerns human monoclonal antibodies of the IgG isotype against human pancreatic islet cells which can be obtained by immortalizing human lymphocytes of prediabetics or diabetics, treating the culture supernatant of the immortalized cells with a conjugate of antibodies against human Fc &ggr; and a label, subsequently treating with human immunoglobulin, incubating with immobilized human pancreatic islet cells identifying an immortalized human cell culture which produces an antibody against pancreatic islet cells via determination of the label bound to the immobilized islet cells, isolating a human immortalized cell which produces this antibody, propagating this immortalized cell and isolating the monoclonal antibody produced by these cells. The invention also concerns a process for the isolation of an islet cell antigen to which such antibodies bind as well as a method for the determination of antibodies against an islet cell antigen of the pancreas.

Abstract: A device for separating layers of laminate trim includes a pair of nip rollers defining a feed path for a laminate, at least two winders and a drive system for the winders. The winders are preferably arranged on an upstream side of the nip rollers. The device can be provided at the output of a lamination machine to receive longitudinal trim portions cut from a laminate being formed by the lamination machine, so as to provide continuous delamination of trim exiting the lamination machine. In the case of a three (or multiple) layer laminate, the device can include a third winder (or additional winders) for collecting a middle layer of the laminate.

Abstract: A differential calorimeter includes sample and reference cells, a thermal shield surrounding these cells, heating devices thermally coupled to the thermal shield and the cells, a temperature monitoring system for monitoring temperature of the shield, cell temperatures and temperature differentials between the cells and the shield, and a control system. The control system has input lines to receive signals from the temperature sensors and output lines connected to the heating devices. The control system is configured to generate output signals for control of heating devices. A gain setting and scan rate are selected by means of a user interface. The output control signals are functions of input temperature signals and the user-selected gain setting, and also functions of input temperature signals and the user-selected scan rate using a mapping function stored in memory.

Abstract: A device for making a personal computer child proof is disclosed. The device includes two mirror image adhesive-backed mounting plates and one guard. One mounting plate is adhered to one side of the personal computer case. The second mounting plate is adhered to the opposite side of the personal computer case. The guard is fed through both mounting plates and snapped into the deployed position. To disable the guard, the adult need only unhook the guard and feed it clear from both mounting plates.

Abstract: The invention concerns human monoclonal antibodies of the IgG isotype against human pancreatic islet cells which can be obtained by immortalizing human lymphocytes of prediabetics or diabetics, treating the culture supernatant of the immortalized cells with a conjugate of antibodies against human Fc .gamma. and a label, subsequently treating with human immunoglobulin, incubating with immobilized human pancreatic islet cells identifying an immortalized human cell culture which produces an antibody against pancreatic islet cells via determination of the label bound to the immobilized islet cells, isolating a human immortalized cell which produces this antibody, propagating this immortalized cell and isolating the monoclonal antibody produced by these cells.The invention also concerns a process for the isolation of an islet cell antigen to which such antibodies bind as well as a method for the determination of antibodies against an islet cell antigen of the pancreas.

Abstract: A calorimeter includes sample and reference cells, a thermal shield surrounding these cells, a heating device thermally coupled to the thermal shield, temperature sensors for monitoring a temperature of the shield and a temperature differential between the shield and the cells, and a control system. The control system has an output line connected to the heating device and input lines to receive signals from the temperature sensors. The control system is configured to generate on its output line an output signal which is a function of both the input signals received on its input lines.

Abstract: The present invention provides a human monoclonal antibody against melanoma, characterized in that it binds to the gangliosides GM3 and GD3 but essentially does not bind to the gangliosides GM1, GM2, GD1a, GD1b and GD2, the binding of the antibody to the gangliosides having been determined by immune staining after thin layer chromatographic separation of the gangliosides. The present invention also provides a process for the production of human monoclonal antibodies directed against melanoma, wherein, without previous immunization, B-lymphocytes are isolated from a healthy person, the isolated B-lymphocytes are immortalized, antibodies from the immortalized B-lymphocytes are screened by immune-histochemical analysis for binding against melanoma and/or melanoma metastases, the positively reacting B-lymphocytes are selected, cultured and monoclonal antibodies obtained therefrom.

Abstract: The spectrographic sample cell for the analysis of trace impurities in sample gases. The cell is provided with a gas inlet, a gas outlet and a volume for confining the sample gases for analysis. Two mirrors are provided, a first mirror proximate the gas inlet the second mirror proximate the gas outlet, the first mirror having a hole at its radial center. A conically-shaped cell profile is further provided such that the formation of vortices of sample gas is prevented in the cell upstream of or in the region where the analysis of trace impurities is to take place.