Abstract: The present invention is generally directed to a hierarchical genome assembly process for producing high-quality de novo genome assemblies. The method utilizes a single, long-insert, shotgun DNA library in conjunction with Single Molecule, Real-Time (SMRT®) DNA sequencing, and obviates the need for additional sample preparation and sequencing data sets required for previously described hybrid assembly strategies. Efficient de novo assembly from genomic DNA to a finished genome sequence is demonstrated for several microorganisms using as little as three SMRT® cells, and for bacterial artificial chromosomes (BACs) using sequencing data from just one SMRT® Cell. Part of this new assembly workflow is a new consensus algorithm which takes advantage of SMRT® sequencing primary quality values, to produce a highly accurate de novo genome sequence, exceeding 99.999% (QV 50) accuracy.

Abstract: Systems and methods for analyzing first and second strings against a ground truth string are provided. A construct representing a plurality of components is obtained, each component for a different portion of the truth string. The construct comprises a plurality of measurement string sampling pools each having an identifier and a corresponding plurality of measurement samplings corresponding to one or two of the components. Each sampling has the identifier and a portion of the first or second string. Samplings are assigned to first, second or third classes when coding a portion of the first string, second string, or both the first and second string. First and second positions are tested for sequence events by calculating a plurality of sequence event models using assumptions on the components having samplings encompassing the first and second positions and class assignments. These assumptions are updated using the calculated models and the models are recalculated.

Abstract: A method of optimizing computer-implemented building design, includes the following: defining one or more options for each building component; providing an energy use intensity versus cost optimization value for each option for a plurality of metrics; selecting a subset of the plurality of metrics applicable to each option; defining a metric vector for each metric through connecting the energy use intensity versus cost optimization value for each option; arranging each metric vector on a coordinate grid with an equal angle between each metric vector; constructing a two-dimensional polygon on an XY-plane by interconnecting for all the metric vectors the energy use intensity versus cost optimization value for each option; providing a performance value for each option for each metric vector based on a percentage the metric vector the associated option represents; and representing a fitness factor for each option as a function of each of the plurality of metrics.

Abstract: There is provided a mold apparatus and process whereby a warhead body can be fabricated in a single over molding process which may also include therein metal fragments, metal balls, obturators, boat tails and other aerodynamic features, metal rings and/or threads. Other molds and processes are presented whereby a warhead body may be over molded in a polymer matrix in sequential steps which may include adding therein metal balls, then fragments, and any of obturators, boat tails and other aerodynamic features, metal rings and/or threads, as desired. Still another process for fabricating a warhead body is presented whereby an insert section is first preloaded with metal fragments and covered with a skin, then the insert section is inserted into an oversized area in a mold, and polymer is then loaded around the insert section to form a unitary warhead body.

Abstract: A visualization system comprising a persistent memory, storing a dataset, and a non-persistent memory implements a pattern visualizing method. The dataset contains discrete attribute values for each first entity of a first type in a plurality of first entities of the first type and discrete attribute values for each first entity of a second type in a plurality of first entities of the second type for each second entity in a plurality of second entities. The dataset is compressed by blocked compression and represents discrete attribute values in both compressed sparse row and column formats. The discrete attribute values are clustered to assign each second entity to a cluster in a plurality of clusters.

Abstract: A method for providing an interactive interface for live event outcome selection and prediction may include generating a set of cells for an event. The set of cells may be provided to a client device to present in a user interface. A selection of a cell may be received from the client device. The selected cell may be assigned to a user account. The method may generate a coordinate for each cell in the set, wherein each cell coordinate includes a plurality of dimensions, each dimension corresponding to a different entity of the event, and wherein each cell coordinate is unique for the set of cells. The method may comprise calculating, prior to the start of the event, a probability that an event result represented by a cell coordinate will occur; and presenting the probability in association with the cell on the user interface. Other embodiments are described and claimed.

Abstract: Systems and methods for analyzing first and second strings against a ground truth string are provided. A construct representing a plurality of components is obtained, each component for a different portion of the truth string. The construct comprises a plurality of measurement string sampling pools each having an identifier and a corresponding plurality of measurement samplings corresponding to one or two of the components. Each sampling has the identifier and a portion of the first or second string. Samplings are assigned to first, second or third classes when coding a portion of the first string, second string, or both the first and second string. First and second positions are tested for events by calculating a plurality of event models using assumptions on the components having samplings encompassing the first and second positions and class assignments. These assumptions are updated using the calculated models and the models are recalculated.

Abstract: A method for providing an interactive interface for live event outcome selection and prediction may include generating a set of cells for an event. The set of cells may be provided to a client device to present in a user interface. A selection of a cell may be received from the client device. The selected cell may be assigned to a user account. The method may generate a coordinate for each cell in the set, wherein each cell coordinate includes a plurality of dimensions, each dimension corresponding to a different entity of the event, and wherein each cell coordinate is unique for the set of cells. The method may comprise calculating, prior to the start of the event, a probability that an event result represented by a cell coordinate will occur; and presenting the probability in association with the cell on the user interface. Other embodiments are described and claimed.

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

Abstract: Systems and methods for analyzing first and second strings against a ground truth string are provided. A construct representing a plurality of components is obtained, each component for a different portion of the truth string. The construct comprises a plurality of measurement string sampling pools each having an identifier and a corresponding plurality of measurement samplings corresponding to one or two of the components. Each sampling has the identifier and a portion of the first or second string. Samplings are assigned to first, second or third classes when coding a portion of the first string, second string, or both the first and second string. First and second positions are tested for sequence events by calculating a plurality of sequence event models using assumptions on the components having samplings encompassing the first and second positions and class assignments. These assumptions are updated using the calculated models and the models are recalculated.

Abstract: Systems and methods for analyzing first and second strings against a ground truth string are provided. A construct representing a plurality of components is obtained, each component for a different portion of the truth string. The construct comprises a plurality of measurement string sampling pools each having an identifier and a corresponding plurality of measurement samplings corresponding to one or two of the components. Each sampling has the identifier and a portion of the first or second string. Samplings are assigned to first, second or third classes when coding a portion of the first string, second string, or both the first and second string. First and second positions are tested for events by calculating a plurality of event models using assumptions on the components having samplings encompassing the first and second positions and class assignments. These assumptions are updated using the calculated models and the models are recalculated.

Abstract: A munition is rendered less sensitive to inadvertent initiations by providing a polymer liner on the entire inside surface of the munition. A thermoplastic liner may be fabricated totally within an empty inert munition casing by spraying a layer of electrostatically charged low melt thermoplastic material particles over substantially the entire inside surfaces of the munition casing, which casing is first electrically grounded. After thickening the particle layer through heat, further such layers may be then applied there over, until a desired cumulative thickness liner is fabricated.

Abstract: A system and method for assisted EVA self-return is provided herein. The system estimates a crewmember's navigation state relative to a fixed location, for example on an accompanying orbiting spacecraft, and computes a guidance trajectory for returning the crewmember to that fixed location. The system may account for safety and clearance requirements while computing the guidance trajectory. According to at least one embodiment, the system provides a directional cue (e.g., a visual, auditory, or tactile cue) to a crewmember corresponding to the prescribed trajectory back to the fixed location. The system may be activated by the crewmember or remotely by another crewmember and/or system.

Abstract: A system and method for assisted EVA self-return is provided herein. The system estimates a crewmember's navigation state relative to a fixed location, for example on an accompanying orbiting spacecraft, and computes a guidance trajectory for returning the crewmember to that fixed location. The system may account for safety and clearance requirements while computing the guidance trajectory. According to at least one embodiment, the system actuates the crewmember's safety jetpack to follow the prescribed trajectory to the fixed location.

Abstract: The present disclosure relates to methods, compositions and systems for haplotype phasing and copy number variation assays. Included within this disclosure are methods and systems for combining the barcode comprising beads with samples in multiple separate partitions, as well as methods of processing, sequencing and analyzing barcoded samples.

Abstract: Computer implemented methods, and systems performing such methods for processing signal data from analytical operations and systems, and particularly in processing signal data from sequence-by-incorporation processes to identify nucleotide sequences of template nucleic acids and larger nucleic acid molecules, e.g., genomes or fragments thereof.

Abstract: A system and method for assisted EVA self-return is provided herein. The system estimates a crewmember's navigation state relative to a fixed location, for example on an accompanying orbiting spacecraft, and computes a guidance trajectory for returning the crewmember to that fixed location. The system may account for safety and clearance requirements while computing the guidance trajectory. According to at least one embodiment, the system actuates the crewmember's safety jetpack to follow the prescribed trajectory to the fixed location. In another embodiment, the system provides the crewmember with a directional cue (e.g., a visual, auditory, or tactile cue) corresponding to the prescribed trajectory back to the fixed location. The system may be activated by the crewmember or remotely by another crewmember and/or system.

Abstract: Described are computer-implemented methods, systems, and media for de novo phased diploid assembly of nucleic acid sequence data generated from a nucleic acid sample of an individual utilizing nucleic acid tags to preserve long-range sequence context for the individual such that a subset of short-read sequence data derived from a common starting sequence shares a common tag. The phased diploid assembly is achieved without alignment to a reference sequence derived from organisms other than the individual. The methods, systems, and media described are computer-resource efficient, allowing scale-up.

Abstract: A system and method for assisted EVA self-return is provided herein. The system estimates a crewmember's navigation state relative to a fixed location, for example on an accompanying orbiting spacecraft, and computes a guidance trajectory for returning the crewmember to that fixed location. The system may account for safety and clearance requirements while computing the guidance trajectory. According to at least one embodiment, the system actuates the crewmember's safety jetpack to follow the prescribed trajectory to the fixed location. In another embodiment, the system provides the crewmember with a directional cue (e.g., a visual, auditory, or tactile cue) corresponding to the prescribed trajectory back to the fixed location. The system may be activated by the crewmember or remotely by another crewmember and/or system.

Abstract: A method for providing an interactive interface for live event outcome selection and prediction may include generating a set of cells for an event. The set of cells may be provided to a client device to present in a user interface. A selection of a cell may be received from the client device. The selected cell may be assigned to a user account. The method may generate a coordinate for each cell in the set, wherein each cell coordinate includes a plurality of dimensions, each dimension corresponding to a different entity of the event, and wherein each cell coordinate is unique for the set of cells. The method may comprise calculating, prior to the start of the event, a probability that an event result represented by a cell coordinate will occur; and presenting the probability in association with the cell on the user interface. Other embodiments are described and claimed.