Publication number: 20200283757
Abstract: The present disclosure relates to a method of generating an antibody library, not limiting to a human naïve antibody gene expression library encompassing a pool of nucleic acid sequences derived from a natural antibody repertoire comprising humoral immunity from healthy and genetically diverse human populations. More specifically, the method employs a combination of phage and/or yeast antibody surface display concept which allows to screen large antibody library size and facilitates better folding of antibody structure. The present disclosure also relates to a human naïve antibody library generated by employing the process of the present disclosure, a set of primers employed in the method and application(s) of said antibody library.
Type:
Application
Filed:
December 21, 2015
Publication date:
September 10, 2020
Inventors:
Sohang CHATTERJEE, Kavitha Iyer RODRIGUES, Maloy GHOSH, Sunit MAITY, Divya UNNIKRISHNAN, Yogendra Manjunath BANGALORE MUNIRAJU, Sathyabalan MURUGESAN, Pavithra MUKUNDA, Bhargav PRASAD, Veeresha KAMANAGOWDA, Sanghamitra BHATTACHARJEE, Pravin Kumar DAKSHINAMURTHY, Vivek HALAN, Sankaranarayanan SRINIVASAN, Anuradha HORA, Bairavabalakumar NATARAJAN, Karthika NAIR, Aswini THANIGAIVEL, Amol MALIWALAVE, Bharath Ravindra SHENOY, Sahana Bhima RAO, Subhra Prakash CHAKRABARTY, Ashvini Kumar DUBEY, Amir KHAN, Anurag TIWARI, Santosh KUMAR, Shivani PATEL, Nikitha M