Abstract: Provided are methods for introducing a sequence-specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

Abstract: A clean-in-place system for piping systems uses a pig train having a dry ice and glycol section. The pig train is moved through the piping system as part of a cleaning sequence. The system and method of the disclosure provide an alternative, effective clean-in-place system that significantly reduces water, chemical and energy usage as well as significantly reducing the related environmental impact.

Abstract: A drain plug assembly that has particular application for sealing a drain hole in a high voltage battery compartment on a vehicle. The plug assembly includes a plug that inserted into the drain hole. The plug assembly further includes a return spring coupled to the plug and causing the plug to be biased into the drain hole. The plug assembly also includes at least one shape memory alloy device coupled to the plug and a support structure. The SMA device receives an electrical current that causes the device to contract and move the plug out of the drain hole against the bias of the return spring.

Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

Abstract: As disclosed herein, optimal native genomic loci have been identified in monocot plants, such as maize plants, that represent best sites for targeted insertion of exogenous sequences.

Abstract: As disclosed herein, optimal native genomic loci have been identified in dicot plants, such as soybean plants, that represent best sites for targeted insertion of exogenous sequences.

Abstract: As disclosed herein, optimal native genomic loci from maize plants have been identified that represent best sites for targeted insertion of exogenous sequences.

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

Abstract: In one aspect, the invention relates to compounds having anticancer activity; synthetic methods for making the compounds; pharmaceutical compositions comprising the compounds; and methods of treating disorders associated with uncontrolled cellular proliferation using the compounds and compositions. This abstract is intended to be used as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

Abstract: Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.

Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

Abstract: A deployable camera system for a vehicle includes a body defining a cavity therein, and a camera including a housing having an exterior surface. The camera is reversibly transitionable between a stowed position in which the camera is recessed into the cavity and the exterior surface is substantially flush with the body, and a deployed position wherein the camera protrudes from the cavity and the exterior surface is not substantially flush with the body. The deployable camera system includes a first shape memory alloy element transitionable between a first state and a second state in response to a first thermal activation signal.

Abstract: A pumping assembly is provided. The assembly includes a first movable element selectively movable within a first pump housing and an actuator including an active material. The active material is configured to undergo a change in attribute in response to an activation signal. The active material is operatively connected to the first movable element such that the change in attribute causes the first movable element to move within the first pump housing. In one example, the active material is a shape memory alloy material having a crystallographic phase that is changeable between Austenite and Martensite in response to the activation signal.

Abstract: A protective device for a vehicle wiper assembly is provided. The protective device is configured to selectively at least partially cover the wiper assembly and prevent the wiper assembly from being pulled away from the vehicle. For example, the protective device may prevent entanglement of the wiper assembly with external elements such as those found in automatic car wash facilities. The device may include a member movable between first and second positions such that the member in the second position is configured to at least partially cover or encapsulate the wiper assembly. An active material actuator may be used to move the member between the first and second positions. In another embodiment, the device includes a member operatively connected to and configured to stiffen a pivot point of the wiper assembly.

Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Abstract: A browser extension relates to a Web application such as QuickBooks Online that manages data records, for example, data records about sales transactions. The extension allows a user to store, at a different Web server or other repository, supplemental records relating to particular data records of the Web application; for example, the user, when displaying a sales-transaction data record in the Web application's display window, could attach and/or view a scanned copy of the physical invoice document for the transaction, even if the Web application does not provide that capability natively. When the user is displaying data from the Web application's data record concerning that sales transaction, the browser extension displays, in an auxiliary display area, information about the supplemental records for that transaction's data record, such as (for example) the number of scanned documents “attached” to that data record, and allows the user to view copies of documents on the screen.

Abstract: The present invention is directed to a novel poly(diol citrates)-based nanocomposite materials created using completely biodegradable and biocompatible polymers that may be used in tissue engineering. More specifically, the specification describes methods and compositions for making and using nanocomposites comprised of citric acid copolymers and polymers including but not limited to poly(L-lactic acid) (PLLA) and poly(lactic-co-glycolic acid) (PLGA).

Abstract: A secondary barrier for use in conjunction with an isolation device in a portion of a non-vertical wellbore comprises: a swellable material, wherein the swellable material: (A) has dimensions such that, prior to swelling, the secondary barrier is capable of being placed into the portion of the non-vertical wellbore, and (B) fills a void within the portion of the non-vertical wellbore, wherein the swellable material fills the void after the swelling of at least a portion of the swellable material has occurred, wherein the secondary barrier is positioned adjacent to the isolation device in the portion of the non-vertical wellbore. A method of creating a secondary barrier comprises: introducing the secondary barrier into the portion of the non-vertical wellbore; and contacting or allowing the swellable material to come in contact with the swelling fluid or the increase in temperature.