Abstract: The present invention relates to a novel method for the preparation of a pooled or mixed population of cryopreserved cells (e.g. hepatocytes). In particular, the invention entails the rapid thaw of cells (e.g. hepatocytes), donated from a single individual, which are mixed to create a heterogeneous population and then cryopreserved. The invention also concerns preparations of multi-cryopreserved cells to increase viability prior to immediate use. The process entails reducing exposure to chemical and physical stresses to increase the resultant number of viable cells.

Abstract: A device and method for extending the lifespan of a shaft seal for an open-drive compressor is provided. The device and method can also reduce and/or prevent deterioration of the shaft seal regardless of the operation condition of the open-drive compressor. The device and method can further reduce and/or prevent leakage of a lubricant and/or refrigerant that can cause deterioration of components within a transport refrigeration unit (TRU).

Abstract: Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.

Abstract: The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.

Abstract: A device and method for extending the lifespan of a shaft seal for an open-drive compressor is provided. The device and method can also reduce and/or prevent deterioration of the shaft seal regardless of the operation condition of the open-drive compressor. The device and method can further reduce and/or prevent leakage of a lubricant and/or refrigerant that can cause deterioration of components within a transport refrigeration unit (TRU).

Abstract: A method and a continuous passive motion device for providing a dynamically loaded force and a static progressive force to a joint of a patient.

Abstract: The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.

Abstract: The present invention relates to a novel method for the preparation of a pooled or mixed population of cryopreserved cells (e.g. hepatocytes). In particular, the invention entails the rapid thaw of cells (e.g. hepatocytes), donated from a single individual, which are mixed to create a heterogeneous population and then cryopreserved. The invention also concerns preparations of multi-cryopreserved cells to increase viability prior to immediate use. The process entails reducing exposure to chemical and physical stresses to increase the resultant number of viable cells.

Abstract: Metal oxide nanoparticle-containing activated carbon cloth and methods for using such cloths for decontamination and protection from hazardous chemical agents are described herein.

Abstract: A device and method for extending the lifespan of a shaft seal for an open-drive compressor is provided. The device and method can also reduce and/or prevent deterioration of the shaft seal regardless of the operation condition of the open-drive compressor. The device and method can further reduce and/or prevent leakage of a lubricant and/or refrigerant that can cause deterioration of components within a transport refrigeration unit (TRU).

Abstract: Automatic trend analysis is provided on a dataset. Upon selection of a data cell or chart point within a data representation, a trend chart is automatically generated in order to demonstrate how the data within the selection trends over time. The system may automatically configure the trend chart based on the metadata of a data source associated with a selected data such that no input or configuration is needed by a user. The system may identify the time hierarchies and other data attributes included in the metadata of the data source associated with a selected data cell and may automatically configure the axis settings and the trend lines according to metadata of the data source. The trend chart may also be configured to display multiple trend lines for comparing multiple data set trends over a specified time period.

Abstract: A method and a continuous passive motion device for providing a dynamically loaded force and a static progressive force to a joint of a patient

Abstract: An energy management system facilitates the transfer of high frequency energy coupled into an implanted lead at a selected RF frequency or frequency band, to an energy dissipating surface. This is accomplished by conductively coupling the implanted lead to the energy dissipating surface through an energy diversion circuit including one or more passive electronic network components whose impedance characteristics are at least partially tuned to the implanted lead's impedance characteristics.

Abstract: Systems, methods, and computer interfaces for providing value assessment for a user that may involve interconnected computer hardware configured in a particular manner for receiving or suggesting a plurality of life aspects; receiving evaluation input data for each of a plurality of life aspects, wherein the evaluation input data indicates a level of satisfaction for the respective life aspect; receiving or suggesting a plurality of negative triggers; for each of the plurality of negative triggers, receiving or suggesting a positive counter to the respective negative trigger; identifying a plurality of values based on the positive counters to the plurality of negative triggers; for each combination of a value of the plurality of values and a life aspect of the plurality of life aspects, receiving value assessment input data that indicates how present the respective value is within the respective life aspect; and generating value assessment results based on the received value assessment input data and the eval

Abstract: A method and apparatus for performing accurate measurements of the magnetic properties of magnetic nanoparticles (MNPs) in both liquid media and biological matrices for providing information on their size, size distribution and concentration in these media and matrices and, resulting in parameters that influence their functionality and effectiveness.

Abstract: An energy management system that facilitates the transfer of high frequency energy induced on an implanted lead or a leadwire includes an energy dissipating surface associated with the implanted lead or the leadwire and at least one non-linear circuit element switch for diverting energy in the implanted lead or the leadwire to the energy dissipating surface. In alternate configurations, the switch may be disposed so that it electrically opens the implanted lead or the leadwire when diverting energy to the energy dissipating surface. The non-linear circuit element switch is typically a PIN diode.

Abstract: A method and apparatus for performing accurate measurements of the magnetic properties of magnetic nanoparticles (MNPs) in both liquid media and biological matrices for providing information on their size, size distribution and concentration in these media and matrices and, resulting in parameters that influence their functionality and effectiveness.

Abstract: An energy management system that facilitates the transfer of high frequency energy induced on an implanted lead or a leadwire includes an energy dissipating surface associated with the implanted lead or the leadwire, a diversion or diverter circuit associated with the energy dissipating surface, and at least one non-linear circuit element switch for diverting energy in the implanted lead or the leadwire through the diversion circuit to the energy dissipating surface. In alternate configurations, the switch may be disposed between the implanted lead or the leadwire and the diversion circuit, or disposed so that it electrically opens the implanted lead or the leadwire when diverting energy through the diversion circuit to the energy dissipating surface. The non-linear circuit element switch is typically a PIN diode. The diversion circuit may be either a high pass filter or a low pass filter.

Abstract: An energy management system that facilitates the transfer of high frequency energy induced on an implanted lead or a leadwire includes an energy dissipating surface associated with the implanted lead or the leadwire, a diversion or diverter circuit associated with the energy dissipating surface, and at least one non-linear circuit element switch for diverting energy in the implanted lead or the leadwire through the diversion circuit to the energy dissipating surface. In alternate configurations, the switch may be disposed between the implanted lead or the leadwire and the diversion circuit, or disposed so that it electrically opens the implanted lead or the leadwire when diverting energy through the diversion circuit to the energy dissipating surface. The non-linear circuit element switch is typically a PIN diode. The diversion circuit may be either a high pass filter or a low pass filter.

Abstract: A method and apparatus for performing accurate measurements of the magnetic properties of magnetic nanoparticles (MNPs) in both liquid media and biological matrices for providing information on their size, size distribution and concentration in these media and matrices and, resulting in parameters that influence their functionality and effectiveness.