Patents by Inventor Ron Weiss

Ron Weiss has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210095287
    Abstract: The present disclosure, at least in part, relates to a miRNA based logic gate that comprises an engineered RNA carrier that comprises an nuclear export signal, a target site for a first miRNA and a pre-miRNA sequence for a second miRNA. Also provided by the disclosure are recombinant viruses (e.g., recombinant adeno-associated viruses (rAAV)) for delivery of the miRNA based logic gates.
    Type: Application
    Filed: September 25, 2020
    Publication date: April 1, 2021
    Applicant: Massachusetts Institute of Technology
    Inventors: Ron Weiss, Giulio Alighieri
  • Publication number: 20210095286
    Abstract: The present disclosure, at least in part, relates to an engineered RNA (e.g., microRNA and sgRNA), in the absence of an input signal, that is engineered to have a large enough energy gap between the formations of a first secondary structure, which is unrecognizable by an actuator, and a second secondary structure, which is recognizable by an actuator (e.g., Drosha and Cas protein).
    Type: Application
    Filed: September 25, 2020
    Publication date: April 1, 2021
    Applicant: Massachusetts Institute of Technology
    Inventors: Ron Weiss, Giulio Alighieri
  • Publication number: 20200362345
    Abstract: The present disclosure is related to an engineered nucleic acid encoding a post-poly A signal RNA 3? to a terminator for expression of protein, and/or non-coding RNA. Also provided herein are methods for reducing epigenetic silencing, genetic modification, transcriptional regulation of the engineered nucleic acid described herein.
    Type: Application
    Filed: May 15, 2020
    Publication date: November 19, 2020
    Applicant: Massachusetts Institute of Technology
    Inventors: Ron Weiss, James J. Collins, Elvira Vitu, Casper Enghuus, Jeremy Jonathan Gam
  • Publication number: 20200325472
    Abstract: Disclosed herein is a subgenomic promoter library derived from alphavirus. Also provided herein are methods of using the subgenomic promoters to produce antibodies and other molecules.
    Type: Application
    Filed: February 7, 2020
    Publication date: October 15, 2020
    Applicant: Massachusetts Institute of Technology
    Inventors: Ron Weiss, Jin Huh
  • Publication number: 20200289629
    Abstract: Disclosed herein are polynucleotides encoding multi-epitope polypeptides and assemblies thereof, and their use for treating or limiting Toxoplasma gondii infection.
    Type: Application
    Filed: June 3, 2020
    Publication date: September 17, 2020
    Inventors: Rima MCLEOD, Kamal EL BISSATI, Ying ZHOU, Jeff ALEXANDER, Lo VANG, Steve REED, Sara M. PAULILLO, Senthil K. RAMAN, Peter BURKHARD, Mariane MELO, Darrel IRVINE, Ron WEISS, Yuan ZHANG, Alessandro SETTE, John SIDNEY, Brian D. LIVINGSTON, Hernan LORENZI
  • Publication number: 20200291428
    Abstract: Provided herein are engineered HSV-1 vectors comprising a modified HSV-1 genome. The engineered HSV-1 vectors can be used to deliver genetic circuits (e.g., up to 100 kb) to cells in vitro or in vivo. Methods of treating or diagnosing a disease (e.g., cancer) using the engineered HSV-1 vectors described herein are also provided.
    Type: Application
    Filed: January 14, 2020
    Publication date: September 17, 2020
    Applicant: Massachusetts Institute of Technology
    Inventors: Ron Weiss, Maria Hottelet Foley, Jin Huh, Ross D. Jones
  • Publication number: 20200283796
    Abstract: Provided herein, in some aspects, are antibody expression systems comprising DNA launched RNA replicons for high level antibody expression. In some embodiments, the antibody is a therapeutic antibody. In some embodiments, the antibody is an immune check point inhibitor. Methods of using the antibody expression system for treating diseases (e.g., cancer) are also provided.
    Type: Application
    Filed: March 5, 2020
    Publication date: September 10, 2020
    Applicant: Massachusetts Institute of Technology
    Inventors: Ron Weiss, Jin Huh, Jacob Becraft
  • Publication number: 20200281994
    Abstract: The present disclosure relates to synthetic oncolytic viruses comprising a lipid nanoparticle comprising one or more types of lipid and a self-amplifying replicon RNA comprising a sequence that encodes an immunomodulatory molecule.
    Type: Application
    Filed: January 10, 2020
    Publication date: September 10, 2020
    Applicants: Massachusetts Institute of Technology, The Ohio State University
    Inventors: Darrell J. Irvine, Karl Dane Wittrup, Ron Weiss, Yingzhong Li, Noor Momin, Yizhou Dong
  • Patent number: 10731153
    Abstract: The disclosure provides novel large serine recombinases and their respective recognition sites, as well as libraries of orthogonal recombinase recognition sites. Uses of the large serine recombinases, recognition sites, and libraries of orthogonal recombinase recognition sites also are provided.
    Type: Grant
    Filed: January 20, 2017
    Date of Patent: August 4, 2020
    Assignees: Massachusetts Institute of Technology, INRIA (French Institute for Research in Computer Science and Automation)
    Inventors: Ron Weiss, Xavier Cesar Duportet, Gregory Batt, Yinqing Li
  • Publication number: 20200230225
    Abstract: Compositions and methods are described for inducing an immune response against an immunogen in humans. The induced immune response is obtained by administering a heterologous prime-boost combination of an in vitro transcribed (IVT) self-replicating RNA (repRNA) and an adenovirus vector. The compositions and methods can be used to provide a protective immunity against a disease, such as a viral infection or a cancer, in humans.
    Type: Application
    Filed: July 27, 2018
    Publication date: July 23, 2020
    Inventors: Ronald VOGELS, Marijn VAN DER NEUT KOLFSCHOTEN, Darrell J. IRVINE, Ron WEISS, Ely Blanton PORTER, Mariane Bandeira MELO, Tasuku KITADA
  • Publication number: 20200224174
    Abstract: The disclosure provides methods for an in vitro evolution technique to identify and characterize mutations in the non-structural genes of an alphavirus replicon that increase the strength and persistence of expression of the replicon genome. Also provided herein are in vivo methods for administering to an animal model a mutated alphavirus replicon that codes for a gene of experimental or therapeutic interest in the subgenome of the alphavirus replicon. The mutations identified herein improve the therapeutic potential of self-replicating RNA, which may have implications for cancer immunotherapy and beyond, e.g., for vaccination or gene therapy.
    Type: Application
    Filed: January 10, 2020
    Publication date: July 16, 2020
    Applicant: Massachusetts Institute of Technology
    Inventors: Darrell J. Irvine, Ron Weiss, Yingzhong Li
  • Publication number: 20200222332
    Abstract: Disclosed herein are lipid nanoparticles having a specific composition, compositions including such lipid nanoparticles, and methods of treatment using such lipid nanoparticles and compositions.
    Type: Application
    Filed: January 10, 2020
    Publication date: July 16, 2020
    Applicant: Massachusetts Institute of Technology
    Inventors: Darrell J. Irvine, Ron Weiss, Tasuku Kitada, Mariane Melo, Yuan Zhang
  • Publication number: 20200063158
    Abstract: The present invention provides compositions and methods for programming mammalian cells to perform desired functions. In particular, the present invention provides compositions and methods for programming stem cells to differentiate into a desired cell type. A quorum sensing systems that regulates the expression of cell fate regulators is introduced into mammalian host cells, such as stem cells. The quorum sensing systems generally comprises vectors that express the components of a bacterial quorum sensing pathway, including proteins which catalyze the synthesis of an autoinducer and a gene encoding a regulatory partner of the autoinducer, and vectors in which genes encoding cell fate regulators are operably linked to a promoter induced by the autoinducer/regulatory partner complex.
    Type: Application
    Filed: July 1, 2019
    Publication date: February 27, 2020
    Inventors: Ron Weiss, Ihor Lemischka, Priscilla E.M. Purnick, Christoph Schaniel, Miles Miller, Patrick Guye
  • Publication number: 20200056189
    Abstract: Provided herein are genetic circuits and cell state classifiers for detecting the microRNA profile of a cell. The cell state classifiers of the present disclosure utilize phosphorylation state of a transcription factor to control classifier output. Kinases and phosphatase pairs that function in phosphorylating or dephosphorylating the transcription factor are integrated into the circuit, their expression tuned by the presence of microRNAs of interest (e.g., in a cell). The genetic circuits and cell state classifiers may be used in various applications (e.g., therapeutic or diagnostic applications).
    Type: Application
    Filed: August 1, 2019
    Publication date: February 20, 2020
    Applicant: Massachusetts Institute of Technology
    Inventors: Ron Weiss, Ross D. Jones, Jin Huh
  • Patent number: 10563207
    Abstract: Provided is a modular construction of synthetic gene circuits in mammalian cells using TALE transcriptional repressors.
    Type: Grant
    Filed: March 6, 2015
    Date of Patent: February 18, 2020
    Inventors: Zhen Xie, Ron Weiss, Yinqing Li, Yun Jiang, Weixi Liao, He Chen
  • Publication number: 20200040338
    Abstract: Provided herein are genetic circuits and cell state classifiers for detecting the microRNA profile of a cell. In some embodiments, the cell state classifiers described herein utilize an endoribonuclease and a self-amplifying RNA molecule for controlling the expression of an output molecule. In some embodiments, the cell state classifiers described herein are encoded on a single RNA transcript, which is then processed to produce individual genetic circuits that function independently. The genetic circuits and cell state classifiers described herein may be used in various applications (e.g., therapeutic or diagnostic applications).
    Type: Application
    Filed: August 1, 2019
    Publication date: February 6, 2020
    Applicant: Massachusetts Institute of Technology
    Inventors: Ron Weiss, Kevin J. Lebo, Jin Huh
  • Patent number: 10435710
    Abstract: Aspects of the present disclosure are directed to methods and compositions for the production of heterogeneous tissue from human induced pluripotent stem (hiPS) cells.
    Type: Grant
    Filed: May 9, 2017
    Date of Patent: October 8, 2019
    Assignee: Massachusetts Institute of Technology
    Inventors: Patrick Guye, Ron Weiss, Mohammad Reza Ebrahimkhani
  • Publication number: 20190282679
    Abstract: Disclosed herein are polynucleotides encoding multi-epitope polypeptides and assemblies thereof, and their use for treating or limiting Toxoplasma gondii infection.
    Type: Application
    Filed: April 14, 2017
    Publication date: September 19, 2019
    Inventors: Rima MCLEOD, Kamal EL BISSATI, Ying ZHOU, Jeff ALEXANDER, Lo VANG, Steve REED, Sara M. PAULILLO, Senthil K. RAMAN, Peter BURKHARD, Mariane MELO, Darrel IRVINE, Ron WEISS, Yuan ZHANG, Alessandro SETTE, John SIDNEY, Brian D. LIVINGSTON, Hernan LORENZI
  • Publication number: 20190151474
    Abstract: Engineered synthetic RNA-based genetic circuits are provided that are regulated exclusively at the post-transcriptional level.
    Type: Application
    Filed: September 8, 2015
    Publication date: May 23, 2019
    Applicants: Massachusetts Institute of Technology, Kyoto University
    Inventors: Ron Weiss, Liliana Wroblewska, Velia Siciliano, Tasuku Kitada, Maria Hottelet Foley, Katie Bodner, Hirohide Saito, Kei Endo, Darrell J. Irvine, Tyler Wagner, Jacob Becraft
  • Patent number: 10210884
    Abstract: Systems and methods facilitating removal of content from audio files are described. A method includes identifying a sound recording in a first audio file, identifying a reference file having at least a defined level of similarity to the sound recording, and processing the first audio file to remove the sound recording and generate a second audio file. In some embodiments, winner-take-all coding and Hough transforms are employed for determining alignment and rate adjustment of the reference file in the first audio file. After alignment, the reference file is filtered in the frequency domain to increase similarity between the reference file and the sound recording. The frequency domain representation (FR) of the filtered version is subtracted from the FR first audio and the result converted to a time representation of the second audio file. In some embodiments, spectral subtraction is also performed to generate a further improved second audio file.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: February 19, 2019
    Assignee: Google LLC
    Inventors: Richard Francis Lyon, Ron Weiss, Thomas Chadwick Walters