Abstract: Provided herein are genetic circuits and cell state classifiers for detecting the microRNA profile of a cell. In some embodiments, the cell state classifiers described herein utilize an endoribonuclease and a self-amplifying RNA molecule for controlling the expression of an output molecule. In some embodiments, the cell state classifiers described herein are encoded on a single RNA transcript, which is then processed to produce individual genetic circuits that function independently. The genetic circuits and cell state classifiers described herein may be used in various applications (e.g., therapeutic or diagnostic applications).

Abstract: Aspects of the present disclosure are directed to methods and compositions for the production of heterogeneous tissue from human induced pluripotent stem (hiPS) cells.

Abstract: Disclosed herein are polynucleotides encoding multi-epitope polypeptides and assemblies thereof, and their use for treating or limiting Toxoplasma gondii infection.

Abstract: Engineered synthetic RNA-based genetic circuits are provided that are regulated exclusively at the post-transcriptional level.

Abstract: Systems and methods facilitating removal of content from audio files are described. A method includes identifying a sound recording in a first audio file, identifying a reference file having at least a defined level of similarity to the sound recording, and processing the first audio file to remove the sound recording and generate a second audio file. In some embodiments, winner-take-all coding and Hough transforms are employed for determining alignment and rate adjustment of the reference file in the first audio file. After alignment, the reference file is filtered in the frequency domain to increase similarity between the reference file and the sound recording. The frequency domain representation (FR) of the filtered version is subtracted from the FR first audio and the result converted to a time representation of the second audio file. In some embodiments, spectral subtraction is also performed to generate a further improved second audio file.

Abstract: Provided herein are genetic circuits and encoded RNA transcripts that produce an output molecule in response to an RNA cleavage event that removes a degradation signal. In some embodiments, the genetic circuits described herein may be used for detecting RNA cleaver activities (e.g., in a cell). Methods of using the genetic circuits described herein in diagnostic or therapeutic applications are also provided.

Abstract: Provided herein are genetic circuits and cell state classifiers for detecting the microRNA profile of a cell. The cell state classifiers of the present disclosure are designed to incorporate multiple genetic circuits integrated together by transcriptional or translational control. Multiple inputs can be sensed simultaneously by coupling their detection to different portions of the genetic circuit such that the output molecule is produced only when the correct input profile of miRNAs is detected. The genetic circuits and cell state classifiers may be used in various applications (e.g., therapeutic or diagnostic applications).

Abstract: Engineered synthetic RNA-based genetic circuits are provided that are regulated exclusively at the post-transcriptional level.

Abstract: Aspects of the invention relate to reconfigurable chassis that allow for rapid construction and optimization of biocircuits.

Abstract: Aspects of the disclosure relate to synthetic regulatory systems composed of a multifunctional clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) nuclease and at least two distinct guide RNAs (gRNAs). The synthetic regulatory system modulates cleavage and transcription, including repression and activation, in a mammalian cell such as a human cell.

Abstract: The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmRNA molecules.

Abstract: A beverage container comprising a stem having an upper and a lower end, a cup having an upper opening and a lower bowl portion, and a base having a tapered upper end with a diameter that widens evenly along its length towards a lower end until the diameter is at least equal to a diameter of the upper opening of the cup. The upper end of the stem is attached to the cup at a point centered on the bowl portion of the cup. The upper end of the base is removably attached at to the lower end of the stem, the lower end of the base being capable of securely covering the upper opening when not attached to the stem.

Abstract: Aspects of the present disclosure are directed to methods and compositions for the production of heterogeneous tissue from human induced pluripotent stem (hiPS) cells.

Abstract: Aspects herein relate to composition, and related methods, for isolating and assembling DNA molecules without intermediate cloning steps.

Abstract: Systems and methods facilitating removal of content from audio files are described. A method includes identifying a sound recording in a first audio file, identifying a reference file having at least a defined level of similarity to the sound recording, and processing the first audio file to remove the sound recording and generate a second audio file. In some embodiments, winner-take-all coding and Hough transforms are employed for determining alignment and rate adjustment of the reference file in the first audio file. After alignment, the reference file is filtered in the frequency domain to increase similarity between the reference file and the sound recording. The frequency domain representation (FR) of the filtered version is subtracted from the FR first audio and the result converted to a time representation of the second audio file. In some embodiments, spectral subtraction is also performed to generate a further improved second audio file.

Abstract: The disclosure provides novel large serine recombinases and their respective recognition sites, as well as libraries of orthogonal recombinase recognition sites. Uses of the large serine recombinases, recognition sites, and libraries of orthogonal recombinase recognition sites also are provided.

Abstract: The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmRNA molecules.

Abstract: Aspects of the present disclosure are directed to methods and compositions for the production of heterogeneous tissue from human induced pluripotent stem (hiPS) cells.

Abstract: Systems and methods facilitating removal of content from audio files are described. A method includes identifying a sound recording in a first audio file, identifying a reference file having at least a defined level of similarity to the sound recording, and processing the first audio file to remove the sound recording and generate a second audio file. In some embodiments, winner-take-all coding and Hough transforms are employed for determining alignment and rate adjustment of the reference file in the first audio file. After alignment, the reference file is filtered in the frequency domain to increase similarity between the reference file and the sound recording. The frequency domain representation (FR) of the filtered version is subtracted from the FR first audio and the result converted to a time representation of the second audio file. In some embodiments, spectral subtraction is also performed to generate a further improved second audio file.

Abstract: Provided is a modular construction of synthetic gene circuits in mammalian cells using TALE transcriptional repressors.