Abstract: An object of the present invention is to provide a novel approach capable of conveniently producing a large quantity of substantially uniform size cell aggregates. Provided is a method for producing cell aggregates using a cell culture bag, the cell culture bag having a lower face comprising a plurality of recesses, the method comprising the steps of: (1) adding cells and a medium to the cell culture bag, stirring the contents, and culturing the cells while applying a pressure to the cell culture bag; and (2) recovering the formed cell aggregates after the completion of culture.

Abstract: Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same. A multilayer film used for forming a cell culture container, comprising: a base material composed of a polyethylene-based resin having a density of 0.87 g/cm3 to 0.90 g/cm3; and an inner layer composed of a polyethylene-based resin having a density of 0.896 g/cm3 to 0.93 g/cm3 and forming a cell culture icy surface. A cell culture container is formed by using this multilayer film.

Abstract: An object of the present invention is to provide a novel approach capable of conveniently and efficiently removing air that has entered a cell culture bag at the time of addition of a culture medium. The present invention provides a system for culturing cells or cell aggregates using a cell culture bag, the cell culture bag having a lower face comprising a plurality of recesses, the culturing system comprising a mechanism which removes air in the cell culture bag through the cell culture bag by applying a pressure before cell addition to the cell culture bag supplemented with a culture medium.

Abstract: [Object] To provide a vessel suitable for efficient culture and differentiation induction or the like of cells in the same vessel while reducing any contamination risk, and also to provide a cell culture vessel capable of ensuring provision of a flat culture surface over a wide range. [Solving Means] A vessel manufacturing method includes placing a bag-shaped, film-based vessel on a placement stage with concave portions formed on the stage, introducing fluid into the vessel placed on the stage, and pressing the vessel which is placed on the stage, by a pressing member while heating at least one of the stage and the pressing member. Meanwhile, a cell culture vessel includes a first vessel wall as a bottom wall and a second vessel wall. The first vessel wall is formed of a flat film having gas permeability. The second vessel wall is disposed in contact with a peripheral edge portion of the first vessel wall, and has a bulge shape protruding relative to the first vessel wall.

Abstract: A cell culture container includes a container main body comprising a gas-permeable plastic film, and an injection/discharge port. A peripheral portion of the container main body is sealed, and the container main body has a bulging shape on a top surface side of the container main body. A plurality of concave portions serving as cell culture parts are provided on a bottom surface of the container main body.

Abstract: A cell culturing method and device that uses a cell culture bag (1) that includes a bag body (2) configured from an upper surface film (21) and a lower surface film (22) having a sealed periphery, and a port (3) attached to the bag body (2), and a plurality of recesses (4) being formed in the lower surface film (22). The method including: a closing-off step for closing off the plurality of recesses (4) using the upper surface film (21) by discharging a culture medium (S) contained in the bag body (2) through the port (3); and a releasing step for releasing, in some or all of the plurality of closed-off recesses (4), cells or cell aggregates adhering to the inner surfaces of the recesses (4).

Abstract: A grommet is provided with a small-diameter tubular portion that is fixated to an outer circumferential surface of a wire harness, a large-diameter tubular portion that is fixated to a through-hole of a fixed member, and an increasing-diameter tubular portion that connects the small-diameter tubular portion and the large-diameter tubular portion. The increasing-diameter tubular portion includes a small-diameter tubular portion-side end portion provided isolated from the small-diameter tubular portion on the outer circumference side of the small-diameter tubular portion, and a flexible connecting portion connecting an inner circumferential surface of the increasing-diameter tubular portion to the small-diameter tubular portion.

Abstract: A cell culturing method using a cell culturing bag (1) in which a plurality of recess portions are formed, including: a bag body (2) configured from an upper film (21) and a lower film (22) which seal a peripheral portion; and a port (3) mounted on the bag body (2), wherein the cell culturing bag (1) is placed on a placement surface (5a) of a stand (5), and while a culture medium (S) is being injected or discharged from the port (3), a pressing member (6) having a bottom surface (6a) substantially parallel to the placement surface (5a) is used to press the upper film (21) from above.

Abstract: A grommet is provided with a small-diameter tubular portion that is fixated to an outer circumferential surface of a wire harness, a large-diameter tubular portion that is fixated to a through-hole of a fixed member, and an increasing-diameter tubular portion that connects the small-diameter tubular portion and the large-diameter tubular portion. The increasing-diameter tubular portion includes a small-diameter tubular portion-side end portion provided isolated from the small-diameter tubular portion on the outer circumference side of the small-diameter tubular portion, and a flexible connecting portion connecting an inner circumferential surface of the increasing-diameter tubular portion to the small-diameter tubular portion.

Abstract: A cell culture apparatus including cell culture units U and a measurement part (5). The measurement part (5) is provided with an imaging part (51) and is disposed so as to be capable of moving up and down between a back panel part (21) of a casing (2) and a culture container accommodating shelf (32). When the measurement unit (5) reaches a measurement position of each cell culture unit U which is a measurement subject, the culture container accommodating shelf (32) slides toward the measurement unit (5) so that portions to be measured of culture containers U20, U30 in each cell culture unit U come in an imaging range of the imaging part (51).

Abstract: Provided is a mass culture method for cells, which uses a culture vessel having flexibility, can form cell aggregates as needed, and is reduced in contamination risk. A pressure is applied onto a culture vessel, which has flexibility, is filled with cells and a culture fluid, and is placed on a placement surface having one or more depressions. With recessed and projected portions, which conform to the depressions, being formed on and in an outer surface of the culture fluid vessel under the application of the pressure, the cells in the culture vessel are cultured.

Abstract: A counting device counts the number of counting objects in liquid in a flexible container. The counting device includes a mounting table on which the container is mounted and a pressing table arranged opposite to a container-arranged side of the mounting table. The mounting table and the pressing table move relatively in a direction of sandwiching the container, and a light-passing hole facing a surface of the container is provided in at least one of the mounting table and the pressing table. The pressing table includes one or more thickness-setting members that sandwich the container between the pressing table and the mounting table in a periphery of the light-passing hole and on the side opposite to the container in a protruding manner.

Abstract: A vessel manufacturing method includes placing a bag-shaped, film-based vessel on a placement stage with concave portions formed on the stage, introducing fluid into the vessel placed on the stage, and pressing the vessel which is placed on the stage, by a pressing member while heating at least one of the stage and the pressing member. Meanwhile, a cell culture vessel includes a first vessel wall as a bottom wall and a second vessel wall. The first vessel wall is formed of a flat film having gas permeability. The second vessel wall is disposed in contact with a peripheral edge portion of the first vessel wall, and has a bulge shape protruding relative to the first vessel wall. The first vessel wall is flat at its section other than a region where the first vessel wall is in contact with at least a charge/discharge port.