Abstract: Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same. A multilayer film used for forming a cell culture container, comprising: a base material composed of a polyethylene-based resin having a density of 0.87 g/cm3 to 0.90 g/cm3; and an inner layer composed of a polyethylene-based resin having a density of 0.896 g/cm3 to 0.93 g/cm3 and forming a cell culture surface. A cell culture container is formed by using this multilayer film.

Abstract: Cell aggregate formation control and cell aggregate disintegration control are carried out with respect to cells in a culture container to increase cell proliferation efficiency. The number of the cells in the culture container is counted without disassembly of a culture system and irrespective of the density of the cells. An external force is applied to the culture container to carry out at least one of cell aggregate formation control and cell aggregate disintegration control with respect to cells in the culture container, thus culturing the cells. The external force is applied to the culture container by pressing an agitating member onto a top surface of the culture container placed in flat orientation to a predetermined pressing degree, and moving the agitating member in a horizontal direction at a predetermined speed, thus controlling at least one of cell aggregate formation and cell aggregate disintegration with respect to the cells in the culture container.

Abstract: A polyester container obtained by stretch-forming a preform of a thermoplastic polyester resin formed by injection forming wherein the central portion of the bottom wall is the remaining part of the gate at the time of injection forming, and is formed relatively thicker than the bottom wall surrounding the central portion, and the central portion of the bottom wall is substantially amorphous, and the bottom wall surrounding the central portion is oriented and crystallized. The container exhibits excellent heat resistance and shock resistance despite a thick portion that is the remaining portion of the injection gate is formed at the center of the bottom portion.

Abstract: By maintaining the cell density during culture at an appropriate level and eliminating the procedure of transferring cells from a container to another container when a large amount of cells are cultured, damage on cells can be reduced, risk of contamination can be lowered, labor saving can be attained and automation can be attained. A cell culture apparatus including: a culture container 11 formed of a soft packing material and having an enclosure part 11-1 in which a culture medium and/or cells being cultured are enclosed is placed; a container table 12 on which the culture container 11 is placed; a roller 13 which is placed on the upper surface of the culture container 11 and divides the enclosure part 11-1 into two or more chambers; and a driving means 15 which relatively moves the roller 13 or the culture container 11, thereby to change the volume of the culture part 11-11 in the enclosure part 11-1 in which a culture medium or cells being cultured are enclosed.

Abstract: In a cell culture in a sealed cell culture container, gradual deterioration of culture environment is prevented.

Abstract: A container with a flange having excellent heat resistance and impact resistance in the lower part of the barrel portion and having excellent transparency in the wall despite the container is formed by molding an amorphous polyester sheet, and a method of producing the same.

Abstract: A polyester container obtained by stretch-forming a preform of a thermoplastic polyester resin formed by injection forming wherein the central portion of the bottom wall is the remaining part of the gate at the time of injection forming, and is formed relatively thicker than the bottom wall surrounding the central portion, and the central portion of the bottom wall is substantially amorphous, and the bottom wall surrounding the central portion is oriented and crystallized. The container exhibits excellent heat resistance and shock resistance despite a thick portion that is the remaining portion of the injection gate is formed at the center of the bottom portion.

Abstract: A fuel injection device for supplying high-pressure fuel to an internal combustion engine includes a fuel supply pump, a first common rail and a second common rail. High-pressure fuel is directly supplied to the first common rail and then to the second common rail from the first common rail through a connecting passage having an orifice. The high-pressure fuel accumulated in the common rails is supplied to injectors and is injected into cylinders in a controlled manner. To suppress pressure wave propagation from the first common rail to the second common rail while providing an appropriate flow passage size, a passage diameter of the orifice is set to 0.9 mm–1.3 mm. In this manner, a pressure difference between the first common rail and the second common rail is minimized.

Abstract: A fuel injection system includes an injector, a controller, and a cylinder internal pressure sensor. The injector injects fuel to an engine. The controller controls injection start timing of the injector, an injection amount, and a supply pressure of the injected fuel. The cylinder internal pressure sensor detects one of a cylinder internal pressure and a heat release rate of the internal combustion engine. The controller has a standard estimator and a corrector. The standard state estimator obtains a standard state of one of the cylinder internal pressure and the heat release rate upon fuel injection from the injector. The corrector corrects at least one of the injection start timing, the injection amount, and the supply pressure in a direction to eliminate a difference that exists between the standard state obtained by the standard state estimator and one of the cylinder internal pressure and the heat release rate detected by the cylinder internal pressure sensor.

Abstract: When a pressure-feeding period of a supply pump and an injection period of an injector overlap and an actual injection quantity is affected by a pump pressure-feeding quantity of fuel supplied by the supply pump, an engine control unit (ECU) calculates the pump pressure-feeding quantity supplied during the injection period and calculates a correction value in accordance with the pump pressure-feeding quantity. The ECU corrects a command injection quantity with the correction value. Thus, even if injection start timing changes in accordance with a change in an operating state and if the pump pressure-feeding quantity supplied during the injection period changes because of the change in the injection start timing, variation in the actual injection quantity can be inhibited. As a result, the injector can inject an optimum quantity of the fuel.

Abstract: A fuel injection system includes an injector, a controller, and a cylinder internal pressure sensor. The injector injects fuel to an engine. The controller controls injection start timing of the injector, an injection amount, and a supply pressure of the injected fuel. The cylinder internal pressure sensor detects one of a cylinder internal pressure and a heat release rate of the internal combustion engine. The controller has a standard estimation means and a correction means. The standard state estimation means obtains a standard state of one of the cylinder internal pressure and the heat release rate upon fuel injection from the injector. The correction means corrects at least one of the injection start timing, the injection amount, and the supply pressure in a direction to eliminate a difference that exists between the standard state obtained by the standard state estimation means and one of the cylinder internal pressure and the heat release rate detected by the cylinder internal pressure sensor.

Abstract: A container with a flange having excellent heat resistance and impact resistance in the lower part of the barrel portion and having excellent transparency in the wall despite the container is formed by molding an amorphous polyester sheet, and a method of producing the same.

Abstract: A fuel injection device for supplying high-pressure fuel to an internal combustion engine includes a fuel supply pump, a first common rail and a second common rail. High-pressure fuel is directly supplied to the first common rail and then to the second common rail from the first common rail through a connecting passage having an orifice. The high-pressure fuel accumulated in the common rails is supplied to injectors and is injected into cylinders in a controlled manner. To suppress pressure wave propagation from the first common rail to the second common rail while providing an appropriate flow passage size, a passage diameter of the orifice is set to 0.9 mm-1.3 mm. In this manner, a pressure difference between the first common rail and the second common rail is minimized.

Abstract: When a pressure-feeding period of a supply pump and an injection period of an injector overlap and an actual injection quantity is affected by a pump pressure-feeding quantity of fuel supplied by the supply pump, an engine control unit (ECU) calculates the pump pressure-feeding quantity supplied during the injection period and calculates a correction value in accordance with the pump pressure-feeding quantity. The ECU corrects a command injection quantity with the correction value. Thus, even if injection start timing changes in accordance with a change in an operating state and if the pump pressure-feeding quantity supplied during the injection period changes because of the change in the injection start timing, variation in the actual injection quantity can be inhibited. As a result, the injector can inject an optimum quantity of the fuel.