Abstract: To achieve, with a simple configuration and simple work, a configuration in which a wire fixating member with band is mounted to a wire harness in a state capable of movement relative to the wire harness. A wire fixating member with band is wound around a wire harness and includes: a vehicle fixation portion capable of fixating to a vehicle; a band fixation portion provided to the vehicle fixation portion and having an engagement claw inside a band insertion hole; and a band formed in a strip shape extending from the band fixation portion. The band includes, at a location separated from a base end of the band, an engagement groove with which the engagement claw can engage.

Abstract: To enable accurate counting the number of cultured cells without pulling apart cell clusters even if cells are aggregated densely to form three-dimensional cell clusters. An image of cultured cells is acquired, and from this image, an image of cell clusters and an image of individual cells are separated. Based on each of the image of cell clusters and the image of individual cells, the number of cells in the cell cluster and the number of individual cells are calculated.

Abstract: A wire fixating member with band is wound around a wire harness and includes: a vehicle fixation portion capable of fixating to a vehicle; a band fixation portion provided to the vehicle fixation portion and including engagement claws inside a band insertion hole; and a band. The band includes a band main body formed in a strip shape extending from the band fixation portion, the band main body having engagement grooves with which the engagement claws can engage and also being capable of insertion into the band insertion hole; and a position regulating protrusion provided at a position on the band main body separated from a base end of the band main body, the position regulating protrusion regulating insertion displacement of the band main body within the band insertion hole.

Abstract: By maintaining the cell density during culture at an appropriate level and eliminating the procedure of transferring cells from a container to another container when a large amount of cells are cultured, damage on cells can be reduced, risk of contamination can be lowered, labor saving can be attained and automation can be attained. A cell culture apparatus including: a culture container 11 formed of a soft packing material and having an enclosure part 11-1 in which a culture medium and/or cells being cultured are enclosed is placed; a container table 12 on which the culture container 11 is placed; a roller 13 which is placed on the upper surface of the culture container 11 and divides the enclosure part 11-1 into two or more chambers; and a driving means 15 which relatively moves the roller 13 or the culture container 11, thereby to change the volume of the culture part 11-11 in the enclosure part 11-1 in which a culture medium or cells being cultured are enclosed.

Abstract: Adhesive culture cells cultured by using a culture container made of a flexible film can selectively be released from the culture container without causing damage to the cells. A cell release method for releasing, from a culture container, adhesive culture cells cultured adhering to the culture container made of a flexible film includes bringing a surface of the film of the culture container into contact with the adhesive culture cells, applying a pressure to the adhesive culture cells, and releasing the adhesive culture cells from the culture container, wherein the surface of the film faces a surface of the film of the culture container to which the adhesive culture cells adhere.

Abstract: A method for counting counted targets disposed in a liquid enclosed in a container, includes adjusting a thickness of at least a part of the container; setting at least a part of the adjusted part as a measurement target region; and counting a number of counted targets in the measurement target region.

Abstract: A counting device counts the number of counting objects in liquid in a flexible container. The counting device includes a mounting table on which the container is mounted and a pressing table arranged opposite to a container-arranged side of the mounting table. The mounting table and the pressing table move relatively in a direction of sandwiching the container, and a light-passing hole facing a surface of the container is provided in at least one of the mounting table and the pressing table. The pressing table includes one or more thickness-setting members that sandwich the container between the pressing table and the mounting table in a periphery of the light-passing hole and on the side opposite to the container in a protruding manner.

Abstract: To enable accurate counting the number of cultured cells without pulling apart cell clusters even if cells are aggregated densely to form three-dimensional cell clusters. An image of cultured cells is acquired, and from this image, an image of cell clusters and an image of individual cells are separated. Based on each of the image of cell clusters and the image of individual cells, the number of cells in the cell cluster and the number of individual cells are calculated.

Abstract: A wire-harness grommet that is not easily dislodged from a plate while minimizing the increase in its weight and cost is provided. In a grommet, a small-diameter cylindrical portion is connected to a connecting portion and can be bound at its ends to a member penetrating an interior of the small-diameter cylindrical portion. A large-diameter cylindrical portion includes an oblique reduced-diameter portion extending fully circumferentially and obliquely inward from where it is connected to the connecting portion. The small-diameter cylindrical portion includes a deformation limiting portion that protrudes farther than other parts of the small-diameter cylindrical portion so as to oppose the oblique reduced-diameter portion. The deformation limiting portion limits the range of inward deformation of the oblique reduced-diameter portion.

Abstract: A wire accommodating protector includes a protector body and a lid for covering the protector body. Lock lugs are provided on outer peripheral edges of the lid and protrude toward the protector body in a manner that allows the lock lugs to flex. Each of the lock lugs has a lock projection provided thereon. Lock lug insertion holes are formed in sidewalls of the protector body, the lock lug insertion holes extend within and down the full height of the sidewalls and are open at top surfaces of the sidewalls. Each of the lock lug insertion holes has a lock protrusion formed therein. The lid is adapted to interlock with the protector body when the plurality of lock lugs are inserted into the respective plurality of lock lug insertion holes so as to engage the lock projections with the lock protrusions.

Abstract: Bubbles are removed from an observation area when observing the inside of a container that is made of a soft package material and encloses a liquid containing bubbles. Provided is a method in which, when observing the inside of a container that is made of a soft package material and encloses a liquid that contains bubbles, a part of space in the container is set to be an observation area, and the bubbles are pushed outside the observation area by pressing from above a part of the upper surface of the container that is positioned above the observation area by means of a pressing member and by moving the pressing member.

Abstract: A method for collecting cells that have been cultured by using a cell culture kit in which, at least, a culture container for cultivating cells, a cell collection container for collecting cells after cultivation and a waste liquid container for collecting a culture medium after cultivation are linked to one another through a conduit, the method including transferring a culture supernatant that has been cultured from the culture container to the waste liquid container, transferring a concentrated suspension of cells from the culture container to the cell collection container, returning part of the culture supernatant discharged in the waste liquid container to the culture container and allowing cells remaining in the culture container to be suspended in a suspension of cells, and transferring again a suspension of cells in which remaining cells are suspended from the culture container to the cell collection container.

Abstract: A wire accommodating protector includes a protector body and a lid for covering the protector body. Lock lugs are provided on outer peripheral edges of the lid and protrude toward the protector body in a manner that allows the lock lugs to flex. Each of the lock lugs has a lock projection provided thereon. Lock lug insertion holes are formed in sidewalls of the protector body, the lock lug insertion holes extend within and down the full height of the sidewalls and are open at top surfaces of the sidewalls. Each of the lock lug insertion holes has a lock protrusion formed therein. The lid is adapted to interlock with the protector body when the plurality of lock lugs are inserted into the respective plurality of lock lug insertion holes so as to engage the lock projections with the lock protrusions.

Abstract: An electrical connection box includes a bracket insertion member having an insertion hole for receiving an insertion plate of a fixing bracket on an automobile. The insertion hole includes edge insertion holes into which edges of the insertion plate of the fixing bracket are inserted. Additionally, a guide surface is formed on an opening of each edge insertion hole such that the guide surface extends obliquely out from the opining in directions opposite to the direction of insertion of the insertion plate into the insertion hole. Each guide surface extends out in the direction along which the edge insertion holes oppose each other and in directions orthogonal to the direction along which the edge insertion holes oppose each other. The dimension W between the outer edge of each opening and the outer edge of the respective guide surface is no less than the thickness t of the insertion plate.

Abstract: A method for counting counted targets disposed in a liquid enclosed in a container, includes adjusting a thickness of at least a part of the container; setting at least a part of the adjusted part as a measurement target region; and counting a number of counted targets in the measurement target region.

Abstract: A culture bag for culturing adherent cells is provided without requiring a highly clean production environment and a complex production step such as a masking step. An adherent cell culture vessel that is formed of a polyolefin is configured so that part or the entirety of the inner surface of the culture vessel has a static water contact angle of 95° or more, and has an advancing contact angle and a receding contact angle that satisfy the inequality “advancing contact angle?receding contact angle>25°” when water runs down along the inner surface.