Abstract: Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same. A multilayer film used for forming a cell culture container, comprising: a base material composed of a polyethylene-based resin having a density of 0.87 g/cm3 to 0.90 g/cm3; and an inner layer composed of a polyethylene-based resin having a density of 0.896 g/cm3 to 0.93 g/cm3 and forming a cell culture icy surface. A cell culture container is formed by using this multilayer film.

Abstract: A wire housing protector in which a locking mechanism includes an elastic projecting piece provided in a lid with an engagement protrusion on an outer surface of its leading end, and an elastic projecting piece insertion portion that is provided in a side wall of the protector body, and includes an engagement portion that engages with the engagement protrusion, contact surfaces include first engagement surfaces that each extend from the side wall side of the protector body toward a side to which the engagement protrusion protrudes, second engagement surfaces that extend from end portions of the first engagement surfaces toward the lid side, and third engagement surfaces that extend from end portions of the second engagement surfaces toward the side to which the engagement protrusion protrudes.

Abstract: An elastic projecting piece insertion hole is defined by an outer wall portion, an inner wall portion that opposes the outer wall portion across a gap, and a left wall portion and a right wall portion that connect edge portions on both sides of the outer wall portion and the inner wall portion. A first tapered surface whose height gradually decreases from an outer edge toward an inner edge is formed at an upper surface of the outer wall portion. An upper surface of the inner wall portion is set to be lower in height than the outer edge of the upper surface of the outer wall portion. Second tapered surfaces whose height gradually decreases from the height of the outer edge of the upper surface of the outer wall portion toward the height of the upper surface of the inner wall portion are respectively formed at upper surfaces of the left wall portion and the right wall portion.

Abstract: A cell culture container includes a container main body comprising a gas-permeable plastic film, and an injection/discharge port. A peripheral portion of the container main body is sealed, and the container main body has a bulging shape on a top surface side of the container main body. A plurality of concave portions serving as cell culture parts are provided on a bottom surface of the container main body.

Abstract: A wire housing protector having a structure in which the volume of electric wires that can be accommodated can be increased by downsizing the locking mechanism, the workability at the time of assembly can be improved, and whether members in the locking mechanism are engaged properly can be more easily confirmed. A fitting protrusion that protrudes toward a lid side is provided at an upper surface of the side wall of the protector body in the vicinity of the elastic projecting piece insertion hole, and the lid is provided with a fitting protrusion housing hole that passes through the lid at a position corresponding to the fitting protrusion in plan view.

Abstract: A cell culture apparatus in which on an abutting surface (2a) around an opening part of a thermostatic chamber (3) and a cold storage chamber (4) that abuts against packing elements (3b, 4b) when front doors (3a, 4a) are closed, a communication groove (5) is provided that intercommunicates the inside and outside of the thermostatic chamber (3) by a communication groove (51) when a transfer tube (U40) is removed. The shut-off mechanism (5) is formed of: a guide hole (52) that opens on the abutting surface (2a) across the communication groove (51) and is deeper than the communication groove (51); a shutter member (53) which is inserted into the guide hole (52) and moves between the blocking position blocking the communication groove (51) and the opening position opening the communication groove (51); and a spring member (54) energizing the shutter member (53) to the blocking position.

Abstract: A method for removing bubbles from an observation area includes pressing, with a pressing member, a part of an upper surface of the container that is positioned above the observation area, and moving the pressing member so as to push the bubbles outside the observation area.

Abstract: A locking mechanism includes an elastic projecting piece provided with an engagement protrusion at its leading end, and an elastic projecting piece insertion hole including an engagement portion that engages with the engagement protrusion. The elastic projecting piece is inserted into the elastic projecting piece insertion hole while the leading end of the elastic projecting piece is pressed toward a side opposite to a side to which the engagement protrusion protrudes, and then the elastic projecting piece elastically returns, whereby the engagement protrusion engages with the engagement portion, and the lid is held by the protector body. A collapsing rib that is located above and in the vicinity of the engagement portion is provided on a surface, of the elastic projecting piece insertion hole, in which the engagement portion is formed.

Abstract: A recessed jig insertion groove that opens in an upper surface of a side wall of a protector body and extends downward is provided in a surface in which an engagement portion in an elastic projecting piece insertion hole is formed, and a jig engagement portion that is located below the upper surface of the side wall of the protector body and which engages a leading end of a jig that has been inserted into the recessed jig insertion groove is provided in an opposing surface of an elastic projecting piece that opposes the recessed jig insertion groove.

Abstract: A wire housing protector in which a locking mechanism includes an elastic projecting piece provided in a lid with an engagement protrusion on an outer surface of its leading end, and an elastic projecting piece insertion portion that is provided in a side wall of the protector body, and includes an engagement portion that engages with the engagement protrusion, contact surfaces include first engagement surfaces that each extend from the side wall side of the protector body toward a side to which the engagement protrusion protrudes, second engagement surfaces that extend from end portions of the first engagement surfaces toward the lid side, and third engagement surfaces that extend from end portions of the second engagement surfaces toward the side to which the engagement protrusion protrudes.

Abstract: An elastic projecting piece insertion hole is defined by an outer wall portion, an inner wall portion that opposes the outer wall portion across a gap, and a left wall portion and a right wall portion that connect edge portions on both sides of the outer wall portion and the inner wall portion. A first tapered surface whose height gradually decreases from an outer edge toward an inner edge is formed at an upper surface of the outer wall portion. An upper surface of the inner wall portion is set to be lower in height than the outer edge of the upper surface of the outer wall portion. Second tapered surfaces whose height gradually decreases from the height of the outer edge of the upper surface of the outer wall portion toward the height of the upper surface of the inner wall portion are respectively formed at upper surfaces of the left wall portion and the right wall portion.

Abstract: A culture bag for culturing adherent cells is provided without requiring a highly clean production environment and a complex production step such as a masking step. An adherent cell culture vessel that is formed of a polyolefin is configured so that part or the entirety of the inner surface of the culture vessel has a static water contact angle of 95° or more, and has an advancing contact angle and a receding contact angle that satisfy the inequality “advancing contact angle?receding contact angle>25°” when water runs down along the inner surface.

Abstract: A cell culture apparatus including cell culture units U and a measurement part (5). The measurement part (5) is provided with an imaging part (51) and is disposed so as to be capable of moving up and down between a back panel part (21) of a casing (2) and a culture container accommodating shelf (32). When the measurement unit (5) reaches a measurement position of each cell culture unit U which is a measurement subject, the culture container accommodating shelf (32) slides toward the measurement unit (5) so that portions to be measured of culture containers U20, U30 in each cell culture unit U come in an imaging range of the imaging part (51).

Abstract: A cell culture system includes a culture medium container to supply a culture medium to a culture container, two culture containers for cell culture, and a transfer tube to connect these containers, whereby liquid delivery is performed by one pump. The containers are connected to the transfer tube; the one pump is attached to the transfer tube; the second culture container is connected to the transfer tube on both sides of the pump to connect the second culture container and the transfer tube, to inject cells into the first culture container; the culture medium is transferred from the culture medium container to the first culture container by the pump; after the cell culture in the first culture container, a cell suspension is transferred from the first culture container to the second culture container; and the culture medium is transferred from the culture medium container to the second culture container.

Abstract: In a cell culture in a sealed cell culture container, gradual deterioration of culture environment is prevented.

Abstract: Adhesive culture cells cultured by using a culture container made of a flexible film can selectively be released from the culture container without causing damage to the cells. A cell release method for releasing, from a culture container, adhesive culture cells cultured adhering to the culture container made of a flexible film includes bringing a surface of the film of the culture container into contact with the adhesive culture cells, applying a pressure to the adhesive culture cells, and releasing the adhesive culture cells from the culture container, wherein the surface of the film faces a surface of the film of the culture container to which the adhesive culture cells adhere.

Abstract: A method for collecting cells that have been cultured by using a cell culture kit in which, at least, a culture container for cultivating cells, a cell collection container for collecting cells after cultivation and a waste liquid container for collecting a culture medium after cultivation are linked to one another through a conduit is provided. The method includes transferring a culture supernatant that has been cultured from the culture container to the waste liquid container, transferring a concentrated suspension of cells from the culture container to the cell collection container, returning part of the culture supernatant discharged in the waste liquid container to the culture container and allowing cells remaining in the culture container to be suspended in a suspension of cells, and transferring again a suspension of cells in which remaining cells are suspended from the culture container to the cell collection container.