Patents by Inventor Selena Chan

Selena Chan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 7226733
    Abstract: A biological sensor which includes: a porous semiconductor structure comprising a central layer interposed between upper and lower layers, each of the upper and lower layers including strata of alternating porosity; and one or more probes coupled to the porous semiconductor structure, the one or more probes binding to a target molecule, whereby a detectable change occurs in a refractive index of the biological sensor upon binding of the one or more probes to the target molecule. Methods of making the biological sensor and methods of using the same are disclosed, as is a detection device which includes such a biological sensor.
    Type: Grant
    Filed: February 21, 2002
    Date of Patent: June 5, 2007
    Assignee: University of Rochester
    Inventors: Selena Chan, Philippe M. Fauchet, Scott R. Horner, Benjamin L. Miller
  • Publication number: 20070116882
    Abstract: The methods, systems 400 and apparatus disclosed herein concern metal 150 impregnated porous substrates 110, 210. Certain embodiments of the invention concern methods for producing metal-coated porous silicon substrates 110, 210 that exhibit greatly improved uniformity and depth of penetration of metal 150 deposition. The increased uniformity and depth allow improved and more reproducible Raman detection of analytes. In exemplary embodiments of the invention, the methods may comprise oxidation of porous silicon 110, immersion in a metal salt solution 130, drying and thermal decomposition of the metal salt 140 to form a metal deposit 150. In other exemplary embodiments of the invention, the methods may comprise microfluidic impregnation of porous silicon substrates 210 with one or more metal salt solutions 130. Other embodiments of the invention concern apparatus and/or systems 400 for Raman detection of analytes, comprising metal-coated porous silicon substrates 110, 210 prepared by the disclosed methods.
    Type: Application
    Filed: May 18, 2006
    Publication date: May 24, 2007
    Inventors: Selena Chan, Sunghoon Kwon, Narayan Sundararajan
  • Publication number: 20070116605
    Abstract: The methods, systems 400 and apparatus disclosed herein concern metal 150 impregnated porous substrates 110, 210. Certain embodiments of the invention concern methods for producing metal-coated porous silicon substrates 110, 210 that exhibit greatly improved uniformity and depth of penetration of metal 150 deposition. The increased uniformity and depth allow improved and more reproducible Raman detection of analytes. In exemplary embodiments of the invention, the methods may comprise oxidation of porous silicon 110, immersion in a metal salt solution 130, drying and thermal decomposition of the metal salt 140 to form a metal deposit 150. In other exemplary embodiments of the invention, the methods may comprise microfluidic impregnation of porous silicon substrates 210 with one or more metal salt solutions 130. Other embodiments of the invention concern apparatus and/or systems 400 for Raman detection of analytes, comprising metal-coated porous silicon substrates 110, 210 prepared by the disclosed methods.
    Type: Application
    Filed: May 17, 2006
    Publication date: May 24, 2007
    Inventors: Selena Chan, Sunghoon Kwon, Narayan Sundararajan
  • Publication number: 20070059733
    Abstract: The present methods and apparatus concern nucleic acid sequencing by incorporation of nucleotides into nucleic acid strands. The incorporation of nucleotides is detected by changes in the mass and/or surface stress of the structure. In some embodiments of the invention, the structure comprises one or more nanoscale or microscale cantilevers. In certain embodiments of the invention, each different type of nucleotide is distinguishably labeled with a bulky group and each incorporated nucleotide is identified by the changes in mass and/or surface stress of the structure upon incorporation of the nucleotide. In alternative embodiments of the invention only one type of nucleotide is exposed at a time to the nucleic acids. Changes in the properties of the structure may be detected by a variety of methods, such as piezoelectric detection, shifts in resonant frequency of the structure, and/or position sensitive photodetection.
    Type: Application
    Filed: June 2, 2006
    Publication date: March 15, 2007
    Inventors: Narayanan Sundararajan, Andrew Berlin, Mineo Yamakawa, Xing Su, Selena Chan, Tae-Woong Koo
  • Publication number: 20060281119
    Abstract: The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nano-barcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nano-barcodes may be any molecule or complex that is distinguishable by SPM, such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and SPM analysis. Compositions comprising coded probes are also disclosed herein. Systems for biomolecule analysis may comprise an SPM instrument and at least one coded probe attached to a surface.
    Type: Application
    Filed: June 2, 2006
    Publication date: December 14, 2006
    Inventors: Selena Chan, Xing Su, Mineo Yamakawa
  • Publication number: 20060215154
    Abstract: The disclosed methods and apparatus concern Raman spectroscopy using metal coated nanocrystalline porous silicon substrates. Porous silicon substrates may be formed by anodic etching in dilute hydrofluoric acid. A thin coating of a Raman active metal, such as gold or silver, may be coated onto the porous silicon by cathodic electromigration or any known technique. In certain alternatives, the metal coated porous silicon substrate comprises a plasma-oxidized, dip and decomposed porous silicon substrate. The metal-coated substrate provides an extensive, metal rich environment for SERS, SERRS, hyper-Raman and/or CARS Raman spectroscopy. In certain alternatives, metal nanoparticles may be added to the metal-coated substrate to further enhance the Raman signals. Raman spectroscopy may be used to detect, identify and/or quantify a wide variety of analytes, using the disclosed methods and apparatus.
    Type: Application
    Filed: October 31, 2005
    Publication date: September 28, 2006
    Inventors: Selena Chan, Andrew Berlin, Sunghoon Kwon, Narayanan Sundararajan, Mineo Yamakawa
  • Patent number: 7105301
    Abstract: The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.
    Type: Grant
    Filed: September 22, 2003
    Date of Patent: September 12, 2006
    Assignee: Intel Corporation
    Inventors: Xing Su, Selena Chan, Tae-Woong Koo, Mineo Yamakawa, Andrew A. Berlin
  • Publication number: 20060199193
    Abstract: Embodiments of the present invention provide devices methods for sequencing DNA using arrays of reaction cavities containing sensors to monitor changes in solutions contained in the reaction cavities. Additional embodiments provide devices and methods for sequencing DNA using arrays of reaction cavities that allow for optical monitoring of solutions in the reaction cavities. Test and fill reaction schemes are disclosed that allow DNA to be sequenced. By sequencing DNA using parallel reactions contained in large arrays, DNA can be rapidly sequenced.
    Type: Application
    Filed: September 13, 2005
    Publication date: September 7, 2006
    Inventors: Tae-Woong Koo, Selena Chan, Xing Su, Zhang Jingwu, Mineo Yamakawa, Val Dubin
  • Publication number: 20060166243
    Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the release of labeled or unlabeled nucleotides that are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides released from a nucleic acid by exonuclease treatment are covalently cross-linked to nanoparticles and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus for nucleic acid sequencing.
    Type: Application
    Filed: January 10, 2006
    Publication date: July 27, 2006
    Inventors: Xing Su, Andrew Berlin, Selena Chan, Steven Kirch, Tac-Woong Koo, Gabi Neubauer, Valluri Rao, Narayanan Sundararajan, Mineo Yamakawa
  • Publication number: 20060134714
    Abstract: Embodiments of the present invention provide devices and methods for detecting, identifying, distinguishing, and quantifying modification states of proteins and peptides using Surface Enhanced Raman (SERS) and Raman spectroscopy. Applications of embodiments of the present invention include, for example, proteome wide modification profiling and analyses with applications in disease diagnosis, prognosis and drug efficacy studies, enzymatic activity profiling and assays.
    Type: Application
    Filed: August 11, 2005
    Publication date: June 22, 2006
    Inventors: Narayan Sundararajan, Lei Sun, Xing Su, Mineo Yamakawa, Zhang Jingwu, Selena Chan, Andrew Berlin, Tae-Woong Koo, Mark Roth, Phil Gafken
  • Publication number: 20060068440
    Abstract: The methods and apparatus disclosed herein concern nucleic acid characterization by enhanced Raman spectroscopy. In certain embodiments of the invention, exonuclease treatment of the nucleic acids results in the release of nucleotides. The nucleotides may pass from a reaction chamber through a microfluidic channel and enter a nanochannel or microchannel. The nanochannel or microchannel may be packed with nanoparticle aggregates containing hot spots for Raman detection. As the nucleotides pass through the nanoparticle hot spots, they may be detected by Raman spectroscopy. Identification of the sequence of nucleotides released from the nucleic acid is used to characterize the nucleic acid, for example by sequencing or identifying the nucleic acid. Other embodiments of the invention concern apparatus for nucleic acid sequencing.
    Type: Application
    Filed: November 8, 2005
    Publication date: March 30, 2006
    Inventors: Selena Chan, Xing Su
  • Patent number: 7019828
    Abstract: Briefly, in accordance with one embodiment of the invention, the intensity of the signals from surface enhanced Raman spectroscopy may be increased by using lithium chloride as an enhancer to activate a metallic structure used for surface enhanced Raman spectroscopy. The increased signal intensity may allow surface enhanced Raman spectroscopy to be utilized to detect individual analytes such as nucleotides, for example in DNA sequencing without requiring a dye or radioactive label.
    Type: Grant
    Filed: March 12, 2003
    Date of Patent: March 28, 2006
    Assignee: Intel Corporation
    Inventors: Xing Su, Lei Sun, Tae-Woong Koo, Selena Chan
  • Publication number: 20060046313
    Abstract: Methods and apparatus are provided for assaying cell samples, which may be living cells, using probes labeled with composite organic-inorganic nanoparticles (COINs) and microspheres with COINs embedded within a polymer matrix to which the probe moiety is attached. COINs intrinsically produce SERS signals upon laser irradiation, making COIN-labeled probes particularly suitable in a variety of methods for assaying cells, including biological molecules that may be contained on or within cells, most of which are not inherently Raman-active. The invention provides variations of the sandwich immunoassay employing both specific and degenerate binding, methods for reverse phase assay of tissue samples and cell microstructures, in solution displacement and competition assays, and the like. Systems and chips useful for practicing the invention assays are also provided.
    Type: Application
    Filed: December 30, 2004
    Publication date: March 2, 2006
    Applicant: Intel Corporation
    Inventors: Mark Roth, Tae-Woong Koo, Andrew Berlin, Selena Chan, Xing Su, Lei Sun
  • Publication number: 20060046311
    Abstract: Methods and apparatus are provided herein for assaying biological samples using probes labeled with composite organic-inorganic nanoparticles (COINs) and microspheres with COINs embedded within a polymer matrix to which the probe moiety is attached. COINs are Raman-active nanoparticles made up of aggregated primary metal crystal particles with Raman-active organic compounds adsorbed on the surface in the junctions of aggregated primary metal crystal particles or embedded in the crystal lattice of the primary metal particles. Since COINs intrinsically produce SERS signals upon laser irradiation, COIN-labeled probes are particularly suitable in a variety of methods for assaying biological molecules, most of which are not inherently Raman-active. The invention provides variations of the sandwich immunoassay employing both specific and degenerate binding, methods for reverse phase assay of tissue samples and cell microstructures, in solution displacement and competition assays, and the like.
    Type: Application
    Filed: August 26, 2004
    Publication date: March 2, 2006
    Applicant: Intel Corporation
    Inventors: Lei Sun, Xing Su, Tae-Woong Koo, Selena Chan
  • Publication number: 20060029969
    Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid 13. Exonuclease 15 treatment of the labeled nucleic acid 13 results in the release of labeled nucleotides 16, 130, which are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides 16, 130 released from a nucleic acid 13 by exonuclease 15 treatment are covalently cross-linked to silver or gold nanoparticles 140 and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERFS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus 10, 100, 210 for nucleic acid sequencing.
    Type: Application
    Filed: September 26, 2005
    Publication date: February 9, 2006
    Inventors: Xing Su, Selena Chan, Andrew Berlin, Tae-Woong Koo, Narayan Sundararajan, Mineo Yamakawa
  • Patent number: 6989897
    Abstract: The disclosed methods and apparatus concern Raman spectroscopy using metal coated nanocrystalline porous silicon substrates. Porous silicon substrates may be formed by anodic etching in dilute hydrofluoric acid. A thin coating of a Raman active metal, such as gold or silver, may be coated onto the porous silicon by cathodic electromigration or any known technique. In certain alternatives, the metal coated porous silicon substrate comprises a plasma-oxidized, dip and decomposed porous silicon substrate. The metal-coated substrate provides an extensive, metal rich environment for SERS, SERRS, hyper-Raman and/or CARS Raman spectroscopy. In certain alternatives, metal nanoparticles may be added to the metal-coated substrate to further enhance the Raman signals. Raman spectroscopy may be used to detect, identify and/or quantify a wide variety of analytes, using the disclosed methods and apparatus.
    Type: Grant
    Filed: October 7, 2003
    Date of Patent: January 24, 2006
    Assignee: Intel Corporation
    Inventors: Selena Chan, Andrew A. Berlin, Sunghoon Kwon, Narayanan Sundararajan, Mineo Yamakawa
  • Publication number: 20060009914
    Abstract: Embodiments of the present invention provide devices and methods for detecting, identifying, distinguishing, and quantifying modifications to nucleic acids, proteins, and peptides using SERS and Raman spectroscopy. Applications of embodiments of the present invention include proteome wide modification profiling and analyses with applications in disease diagnosis, prognosis and drug efficacy studies, enzymatic activity profiling and assays.
    Type: Application
    Filed: August 16, 2004
    Publication date: January 12, 2006
    Inventors: Narayan Sundararajan, Lei Sun, Xing Su, Mineo Yamakawa, Zhang Jingwu, Selena Chan, Andrew Berlin, Tae-Woong Koo, Mark Roth, Phil Gafken
  • Patent number: 6972173
    Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid 13. Exonuclease 15 treatment of the labeled nucleic acid 13 results in the release of labeled nucleotides 16, 130, which are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides 16, 130 released from a nucleic acid 13 by exonuclease 15 treatment are covalently cross-linked to silver or gold nanoparticles 140 and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus 10, 100, 210 for nucleic acid sequencing.
    Type: Grant
    Filed: March 14, 2002
    Date of Patent: December 6, 2005
    Assignee: Intel Corporation
    Inventors: Xing Su, Selena Chan, Andrew A. Berlin, Tae-Woong Koo, Narayan Sundararajan, Mineo Yamakawa
  • Patent number: 6970239
    Abstract: The methods and apparatus 300 disclosed herein concern Raman spectroscopy using metal coated nanocrystalline porous silicon substrates 240, 340. In certain embodiments of the invention, porous silicon substrates 110, 210 may be formed by anodic etching in dilute hydrofluoric acid 150. A thin coating of a Raman active metal, such as gold or silver, may be coated onto the porous silicon 110, 210 by cathodic electromigration or any known technique. The metal-coated substrate 240, 340 provides an extensive, metal rich environment for SERS, SERRS, hyper-Raman and/or CARS Raman spectroscopy. In certain embodiments of the invention, metal nanoparticles may be added to the metal-coated substrate 240, 340 to further enhance the Raman signals. Raman spectroscopy may be used to detect, identify and/or quantify a wide variety of analytes, using the disclosed methods and apparatus 300.
    Type: Grant
    Filed: June 12, 2002
    Date of Patent: November 29, 2005
    Assignee: Intel Corporation
    Inventors: Selena Chan, Andrew A. Berlin, Mineo Yamakawa
  • Publication number: 20050250159
    Abstract: The invention provides methods for analyzing the protein content of a biological sample, for example to obtain a protein profile of a sample provided by a particular individual. The proteins and protein fragments in the sample are separated on the basis of chemical and/or physical properties and maintained in a separated state at discrete locations on a solid substrate or within a stream of flowing liquid. Raman spectra are then detected as produced by the separated proteins or fragments at the discrete locations such that a spectrum from a discrete location provides information about the structure or identity of one or more particular proteins or fragments at the discrete location. The proteins or fragments at discrete locations can be coated with a metal, such as gold or silver, and/or the separated proteins can be contacted with a chemical enhancer to provide SERS spectra. Method and kits for practicing the invention are also provided.
    Type: Application
    Filed: March 17, 2005
    Publication date: November 10, 2005
    Inventors: Xing Su, Lei Sun, Mineo Yamakawa, Tae-Woong Koo, Selena Chan, Andrew Berlin, Narayanan Sundararajan