Abstract: Provided is a method for obtaining a double-stranded sequence by single-stranded rolling circle amplification, comprising: 1) performing rolling circle amplification reaction on single-stranded circular DNA by means of a first primer to obtain an amplified sequence, the first primer being complementary to a partial region of the single-stranded circular DNA, and the single-stranded circular DNA having a break mechanism that can cause the single-stranded circular DNA to ring-open; 2) ring-opening the single-stranded circular DNA by means of the break mechanism to obtain single-stranded linear DNA; and 3) using the single-stranded linear DNA as a second primer and using the amplified sequence obtained in step 1) as a template to perform amplification reaction to obtain an amplified double-stranded sequence.

Abstract: Provided are a magnetic bead-based detection method, a storage medium, and a detection device. The detection method includes: collecting a white light image of a to-be-detected solution, in which the to-be-detected solution is mixed with a to-be-detected sample and magnetic beads with a capture agent (S1); determining magnetic stripe regions in the white light image, and determining first magnetic bead regions based on the magnetic stripe regions (S2); selecting, by using a first neural network, second magnetic bead regions containing magnetic beads from the first magnetic bead regions, and obtaining a marker position of each of the magnetic beads (S3); and obtaining, by using a second neural network and based on each of the second magnetic bead regions, codes at code bits of a corresponding magnetic bead, and obtaining corresponding code information based on the codes of the code bits and the marker position of the magnetic bead (S4).

Abstract: Provided is an underwater wireless optical communication system. The system includes an emitting end and a receiving end; where the emitting end includes a beam emitting module and an optical phased array, where the beam emitting module is configured to emit a communication beam obtained by modulating communication information, and the optical phased array is configured to adjust an emission direction of the communication beam; and the receiving end includes a tracking module and an information demodulation module, where the tracking module is configured to receive a target communication beam emitted after adjustment by the optical phased array and align the target communication beam, and the information demodulation module is configured to demodulate the received target communication beam to obtain the communication information.

Abstract: Provided is an underwater wireless optical communication system. The system includes an emitting end and a receiving end; where the emitting end includes a beam emitting module and an optical phased array, where the beam emitting module is configured to emit a communication beam obtained by modulating communication information, and the optical phased array is configured to adjust an emission direction of the communication beam; and the receiving end includes a tracking module and an information demodulation module, where the tracking module is configured to receive a target communication beam emitted after adjustment by the optical phased array and align the target communication beam, and the information demodulation module is configured to demodulate the received target communication beam to obtain the communication information.

Abstract: Example methods, systems, and apparatuses for verifying a printed indicium are provided. An example method may include receiving a captured image of a print media comprising the printed indicium; extracting a quiet zone grade portion from the captured image, where the quiet zone grade portion includes a printed indicium area of the printed indicium and at least one quiet zone area adjacent to the printed indicium area; in response to receiving a user input providing an overwrite quiet zone requirement indication and determining that the at least one quiet zone area does not satisfy at least one quiet zone requirement, causing at least one of adjusting the at least one quiet zone requirement to at least one reduced quiet zone requirement or adding at least one additional quiet zone area to the at least one quiet zone area.

Abstract: The present invention relates to the field of nucleic acid sequencing. Specifically, provided in the present invention is a nucleotide analogue for sequencing. The nucleotide analogue carries a linker on the base or the ribose ring 3?-OH, and can be used for NGS sequencing. The present invention further relates to a kit containing the nucleotide analogue, and a sequencing method based on the nucleotide analogue.

Abstract: A chimeric DNA polymerase includes: a first fragment having at least 80% homology to at least part of an N-end domain of KOD DNA polymerase; a second fragment having at least 80% homology to at least part of an exonucleolytic domain of Pab DNA polymerase; a third fragment having at least 80% homology to at least part of the N-end domain of KOD DNA polymerase; a fourth fragment having at least 80% homology to at least part of a palm domain of Pfu DNA polymerase; a fifth fragment having at least 80% homology to at least part of a finger domain of Pab DNA polymerase; a sixth fragment having at least 80% homology to at least part of the palm domain of Pfu DNA polymerase; and a seventh fragment having at least 80% homology to at least part of a thumb domain of KOD DNA polymerase.

Abstract: Provided is a visible light communication system. The system includes a modulation and light emission module configured to emit a communication light ray obtained by modulating information to be communicated; and a tracking and light sensing module configured to receive and demodulate the communication light ray to obtain the information to be communicated and further configured to align the tracking and light sensing module with the communication light ray according to the angle between the tracking and light sensing module and the communication light ray.

Abstract: A nucleic acid probe and a nucleic acid sequencing method for performing sequencing while ligating nucleic acids. The nucleic acid probe is a DNA sequencing probe, comprising a first moiety, a second moiety, a linker, and a detectable label. A base of the first moiety is A, T, U, C, or G, a base of the second moiety is a random base and/or a universal base, and 3 bases or more are present in the second moiety. The first moiety and the second moiety are ligated via the linker, the connection between the first moiety and the ligation can be cleaved, and the detectable label is ligated to the second moiety or the linker. The above probe, a combination formed therewith, or a sequencing method using the same can reduce the number or types of probes in nucleic acid sequencing, thereby reducing cost.

Abstract: A ground simulation device and method for an on-orbit manipulation of a space manipulator is provided. The ground simulation device includes: a dual-arm robot, configured to simulate the space manipulator operating a target object; a suspension device, including a fixed post and passive rods, where the passive rods are movably connected with a top end of the fixed post, and the target object is suspended to the passive rods; and a simulation platform, configured to fix the dual-arm robot and the suspension device thereon. The ground simulation device provides the passive rods on the suspension device and suspends the target object to the passive rods, thus overcoming the gravity of the target object. In addition, the passive rods can drive the target object to move under an influence of an external force, achieving a similar suspension effect to that in space, and providing a desired, safe, and reliable implementation effect.

Abstract: Provided is a visible light communication system. The system includes a modulation and light emission module configured to emit a communication light ray obtained by modulating information to be communicated; and a tracking and light sensing module configured to receive and demodulate the communication light ray to obtain the information to be communicated and further configured to align the tracking and light sensing module with the communication light ray according to the angle between the tracking and light sensing module and the communication light ray.

Abstract: Example methods, systems, and apparatuses for verifying a printed indicium are provided. An example method may include receiving a captured image of a print media comprising the printed indicium; extracting a quiet zone grade portion from the captured image, where the quiet zone grade portion includes a printed indicium area of the printed indicium and at least one quiet zone area adjacent to the printed indicium area; in response to receiving a user input providing an overwrite quiet zone requirement indication and determining that the at least one quiet zone area does not satisfy at least one quiet zone requirement, causing at least one of adjusting the at least one quiet zone requirement to at least one reduced quiet zone requirement or adding at least one additional quiet zone area to the at least one quiet zone area.

Abstract: A modified nucleoside or nucleotide, the 3?-OH of the modified nucleoside or nucleotide being reversibly blocked; meanwhile, the present invention also relates to a kit comprising the nucleoside or nucleotide, and a sequencing method based on the nucleoside or nucleotide.

Abstract: Example methods, systems, and apparatuses for verifying a printed indicium are provided. An example method may include receiving a captured image of a print media comprising the printed indicium; extracting a quiet zone grade portion from the captured image, where the quiet zone grade portion includes a printed indicium area of the printed indicium and at least one quiet zone area adjacent to the printed indicium area; in response to receiving a user input providing an overwrite quiet zone requirement indication and determining that the at least one quiet zone area does not satisfy at least one quiet zone requirement, causing at least one of adjusting the at least one quiet zone requirement to at least one reduced quiet zone requirement or adding at least one additional quiet zone area to the at least one quiet zone area.

Abstract: Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.

Abstract: The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing. FIG.

Abstract: A ground simulation device and method for an on-orbit manipulation of a space manipulator is provided. The ground simulation device includes: a dual-arm robot, configured to simulate the space manipulator operating a target object; a suspension device, including a fixed post and passive rods, where the passive rods are movably connected with a top end of the fixed post, and the target object is suspended to the passive rods; and a simulation platform, configured to fix the dual-arm robot and the suspension device thereon. The ground simulation device provides the passive rods on the suspension device and suspends the target object to the passive rods, thus overcoming the gravity of the target object. In addition, the passive rods can drive the target object to move under an influence of an external force, achieving a similar suspension effect to that in space, and providing a desired, safe, and reliable implementation effect.

Abstract: The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing.

Abstract: A method for obtaining a single-cell mRNA sequence, including: (1) capturing mRNA of a cell by using a cell tag carrier, and performing reverse transcription to obtain cDNA having a cell tag; (2) obtaining multiple cDNA fragments having molecular tags by using a transposase complex and a molecular tag carrier; (3) performing high-throughput sequencing; (4) performing sequence assembly according to the molecular tags to obtain the sequence of each mRNA; and (5) obtaining the sequence of all mRNAs of each single cell according to the cell tags.

Abstract: Provided are a method for sequencing polynucleotides based on optical signal kinetics of luminescent labels and secondary luminescent signals, in which different luminescence forms and luminescence timings are applied to distinguish the sequential incorporation of different nucleotides, so as to realize the sequencing of the polynucleotides.