Abstract: Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded with two adaptors to obtain a fragmented product with two adaptors respectively at two ends; and 4) amplifying the fragmented product with two adaptors respectively at two ends using a tag sequence and a pair of primers to obtain said sequencing library.

Abstract: Provided are a method for breaking a nucleic acid and adding an adaptor by means of a transposase, and a reagent.

Abstract: The present invention provides a primer for nucleic acid random fragmentation and a nucleic acid random fragmentation method. The primer consists of a plurality of upstream random primers and downstream random primers. The sequence composition of the upstream random primers is 5?-X-Y-3?, and the sequence composition of the downstream random primers is 5?-P-Y?-X?-close-3?, wherein Y and Y? are random sequences, X is all or part of sequences of a sequencing platform 5? end adaptor, X? is all or part of sequences of a sequencing platform 3? end adaptor, P is phosphorylation modification, and close is close modification. The primer of the present invention adopts double random anchoring of both the upstream random primers and the downstream random primers, and a DNA sample can be randomly broken.

Abstract: Provided are an isolated oligonucleotide and a use thereof in nucleic acid sequencing, wherein the isolated oligonucleotide comprises a first strand, wherein the 5?-end nucleotide of the first strand has a phosphate group, and the 3?-end nucleotide of the first strand is a dideoxynucleotide, and a second strand, wherein the 5?-end nucleotide of the second strand does not have a phosphate group, and the 3?-end nucleotide of the second strand is a dideoxynucleotide, wherein the first strand is longer than the second strand in length, and a double-stranded structure is formed between the first strand and the second strand.

Abstract: Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5? end of the long strand has a phosphoric acid modification, and the 3? end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3? end thereof can be in a complementary pairing with the 5? end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.

Abstract: An integrated circuit (IC) device includes a lead frame having a first and a second opposing surface and a plurality of lead fingers. A first die including a signal processor is mounted on the first surface of the lead frame while a second die is mounted on the second surface of the lead frame. The second die includes at least one sensor that senses at least one non-electrical parameter and has at least one sensor output that provides a sensing signal for the parameter. The sensor output is coupled to the signal processor for processing the sensing signal.

Abstract: An optical keyless entry sensor system and method includes an optical sensor in association with a mirror that reflects light transmitted from the optical sensor, wherein reflected light is detectable by the optical sensor. An attenuation filter can be located between the mirror and the optical sensor, wherein the attenuation filter is configured to simulate a contamination of the optical sensor in order to determine an exact level of attenuation representative of contamination that causes a performance failure of the optical sensor, thereby providing data which is indicative of a dynamic range of the optical sensor, such that the dynamic range is utilized to enhance the performance of the optical keyless entry sensor system.

Abstract: A humidity sensor having temperature sensing includes a substrate and a RLC (damped resonant) circuit formed in or on the substrate. The RLC circuit comprises a capacitor having a first and a second electrically conductive plate and a moisture sensitive dielectric interposed between the first and second plate. An RL network comprising an inductor and a resistor is coupled to the capacitor and comprises a high relative temperature coefficient of resistance (TCR) portion formed from a first material which is coupled to a low relative TCR portion formed from a second material different from the first material. The RLC circuit can be a series RLC circuit. In another embodiment, the RLC circuit can comprises a parallel RLC circuit.

Abstract: A SAW-based humidity sensor apparatus with integrated signal conditioning on the same substrate. A micro-electronic circuit can be processed at a silicon substrate to obtain on-chip signal conditioning internally and the silicon substrate can be covered with a protective layer of, for example, silicon nitride. Surface acoustic wave media (e.g., a ZnO film) can be coated on top of the protective layer. A humidity sensitive film and two sets of interdigital transducers can then be deposited on the surface acoustic wave media. The humidity sensitive film absorbs moisture and changes the receiving frequency at the two sets of interdigital transducers. The output from the two sets of interdigital transducers can be processed by the micro-electronic circuit, which eliminates common mode noise and generates an output signal proportional to the humidity value tested.

Abstract: A system for heating a medical fluid includes a container including a fluid path including an inlet side and an outlet side. A broadband infrared radiation source including a power supply is coupled to a broadband emitter for emitting infrared radiation. The broadband emitter provides a focal location within the fluid path to heat the medical fluid to generate a heated medical fluid. A flow turbulence enhancing structure mixes the heated medical fluid to reduce temperature gradients therein. A fluid detector senses a presence of the medical fluid in the fluid path and generates a fluid sensing signal. A controller is coupled to receive the fluid sensing signal and has an output coupled to the power supply for controlling a power output of the power supply based on the fluid sensing signal indicating a presence of the medical fluid in the container.

Abstract: A system for heating a medical fluid includes a container including a fluid path including an inlet side and an outlet side. A broadband infrared radiation source including a power supply is coupled to a broadband emitter for emitting infrared radiation. The broadband emitter provides a focal location within the fluid path to heat the medical fluid to generate a heated medical fluid. A flow turbulence enhancing structure mixes the heated medical fluid to reduce temperature gradients therein. A fluid detector senses a presence of the medical fluid in the fluid path and generates a fluid sensing signal. A controller is coupled to receive the fluid sensing signal and has an output coupled to the power supply for controlling a power output of the power supply based on the fluid sensing signal indicating a presence of the medical fluid in the container.

Abstract: A system and method for heating a fluid for delivery of the fluid in the context of a particular medical, pharmaceutical, industrial or mechanical application. A cassette is associated with an application, such as, for example, a peritoneal dialysis machine. The cassette includes one or more outlet tubes for delivery of the fluid to, for example, a patient. A heating device is associated with the cassette, and heats a portion of the fluid prior to delivery of the fluid to the patient, thereby saving time and electrical power during delivery of the fluid to the patient. Additionally, a controller can be employed to control the heating range of the heating device and thereby provide a temperature of the fluid that is adaptable to the patient or a particular application.

Abstract: An integrated circuit (IC) device includes a lead frame having a first and a second opposing surface and a plurality of lead fingers. A first die including a signal processor is mounted on the first surface of the lead frame while a second die is mounted on the second surface of the lead frame. The second die includes at least one sensor that senses at least one non-electrical parameter and has at least one sensor output that provides a sensing signal for the parameter. The sensor output is coupled to the signal processor for processing the sensing signal.

Abstract: A SAW-based humidity sensor apparatus with integrated signal conditioning on the same substrate. A micro-electronic circuit can be processed at a silicon substrate to obtain on-chip signal conditioning internally and the silicon substrate can be covered with a protective layer of, for example, silicon nitride. Surface acoustic wave media (e.g., a ZnO film) can be coated on top of the protective layer. A humidity sensitive film and two sets of interdigital transducers can then be deposited on the surface acoustic wave media. The humidity sensitive film absorbs moisture and changes the receiving frequency at the two sets of interdigital transducers. The output from the two sets of interdigital transducers can be processed by the micro-electronic circuit, which eliminates common mode noise and generates an output signal proportional to the humidity value tested.

Abstract: A flexible heater 200 includes at least one hot wire resistive element 215 and a thermally insulating and electrically insulating flex holding material (201, 202) surrounding the resistive element (215) for holding the resistive element (215). A temperature sensor (225) having at least a portion embedded in the holding material is operable for measuring a temperature of at least one location along a length of the resistive element (215). A monitored flexible heater system (600) includes a flexible heater (610) including at least one resistive element, a thermally insulating and electrically insulating flex holding material surrounding the resistive element and a temperature sensor (615) having at least a portion embedded in the holding material operable for measuring a temperature of at least one location along a length of the resistive element.

Abstract: A humidity sensor having temperature sensing includes a substrate and a RLC (damped resonant) circuit formed in or on the substrate. The RLC circuit comprises a capacitor having a first and a second electrically conductive plate and a moisture sensitive dielectric interposed between the first and second plate. An RL network comprising an inductor and a resistor is coupled to the capacitor and comprises a high relative temperature coefficient of resistance (TCR) portion formed from a first material which is coupled to a low relative TCR portion formed from a second material different from the first material. The RLC circuit can be a series RLC circuit. In another embodiment, the RLC circuit can comprises a parallel RLC circuit.

Abstract: A system and method for heating a fluid for delivery of the fluid in the context of a particular medical, pharmaceutical, industrial or mechanical application. A cassette is associated with an application, such as, for example, a peritoneal dialysis machine. The cassette includes one or more outlet tubes for delivery of the fluid to, for example, a patient. A heating device is associated with the cassette, and heats a portion of the fluid prior to delivery of the fluid to the patient, thereby saving time and electrical power during delivery of the fluid to the patient. Additionally, a controller can be employed to control the heating range of the heating device and thereby provide a temperature of the fluid that is adaptable to the patient or a particular application.

Abstract: A strain gauge sensor system and method for measuring the strain in a member, such as a fiber rope, has a sensor target assembly having a plurality of magnets carried spaced apart by individual gauge lengths along the member. Position and trigger sensor devices are configured spaced apart along side a desired path of travel of the member. When the member is fed along the desired path of travel, the trigger sensor device, in response to magnetically sensing a passing magnet, triggers the position sensor device to read the position of an adjacent passing magnet. The strain in the gauge length is determined from the read position. RFID tags placed on the member between the magnets can be identified by an RF reader to identify a particular gauge length being measured. The system can include a deployment device for feeding the member along the desired path.

Abstract: The invention provides an optical liquid level sensor used to determine the level of a liquid within a vessel. The sensor includes two adjacent elongated optical elements, such as optical fibers, which connect a first end, or “sensing end”, to a second end, or “non-sensing end”. The sensing end includes an optical lens for sensing the level of a high temperature liquid, while the non-sensing end includes electronic components for processing optical signal data relating to the sensed liquid level. The ends are sufficiently separated by the elongated optical elements, such that high temperatures exerted on the sensing end when it is immersed in high-temperature liquid are not exerted on the non-sensing end. This configuration avoids the temperature degradation of the electronic components, thus extending the lifetime of the sensor.

Abstract: A barcode sensor package has an optical emitter circuit and an optical detector circuit which are formed as one or more integrated optical circuits disposed in a housing which has a reading surface and one or more apertures located between the optical emitter and detector circuits. The optical emitter circuit has an emitter die, such as a vertical cavity surface emitting laser (VCSEL), for emitting a light beam and a diffraction optical element disposed on the emitter die for focusing the light beam to a bar code. The optical detector circuit has a photodetector die, such as a phototransistor, for detecting reflective light and another diffraction optical element disposed on the photodetector die for guiding light reflected from the bar code to the detector. The diffraction optical elements are fabricated by patterning optical layers, deposited on respective emitter and detector dies, using photolithograph or a direct write process.