Patents by Inventor Wenwei Zhang

Wenwei Zhang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220202984
    Abstract: A germicidal device includes a purification/disinfection and LED lighting member configured to generate UVC light and negative air ions for destroying microorganisms and removing odors in a space in which the purification and sterilization member is placed; a sensing member configured to detect at least one event occurred in said space in real time; and a microcontroller unit coupled with the purification and sterilization member and the sensing member for controlling operations of the purification and sterilization member in a respective state in accordance with the at least one event occurred in said space and energy saving. The UVC light has one or more wavelengths in a range of about 100-400 nm.
    Type: Application
    Filed: March 15, 2022
    Publication date: June 30, 2022
    Inventors: Wenwei Zhang, Wei Li, Yuan Di, Annie You, Low Wee Fong
  • Publication number: 20220205036
    Abstract: The present invention provides a sequencing method based on a single fluorescent dye, in which a self-luminescence signal is used to distinguish the sequential incorporation of different nucleotides, thereby realizing the determination of the polynucleotide sequence.
    Type: Application
    Filed: May 15, 2019
    Publication date: June 30, 2022
    Inventors: Sha LIAO, Xi CHEN, Ao CHEN, Wenwei ZHANG, Chongjun XU, Hongmin CHEN, Jie ZHAO, Defeng FU
  • Publication number: 20220025429
    Abstract: Provided are a rolling circle amplification method, a method for preparing a sequencing library, and a DNA nanoball prepared therefrom. The rolling circle amplification method includes: sequentially denaturing and annealing a double-stranded DNA and a mediating sequence in a same system, to complementarily pair the mediating sequence with two ends of a denatured single-stranded DNA; simultaneously introducing a ligase and a polymerase into the system to connect the two ends of the single-stranded DNA under action of the ligase; and performing a rolling circle amplification reaction under action of the polymerase by using the mediating sequence as a primer and the single-stranded DNA as a template, to obtain DNA nanoball.
    Type: Application
    Filed: December 5, 2018
    Publication date: January 27, 2022
    Inventors: Sha LIAO, Ao CHEN, Wenwei ZHANG, Chongjun XU, Hanjie SHEN, Lin HE, Junqiang XU
  • Publication number: 20220010370
    Abstract: The present invention provides a method for sequencing polynucleotides. Sequential incorporation of different nucleotides is detected by using the same light emitting signal, thereby achieving the determination of polynucleotide sequences.
    Type: Application
    Filed: November 7, 2018
    Publication date: January 13, 2022
    Inventors: Jie ZHAO, Sha LIAO, Wenwei ZHANG, Ao CHEN, Chongjun XU, Defeng FU
  • Publication number: 20210384031
    Abstract: Provided are a chip matrix, a sequencing chip, and a manufacturing method thereof. The chip matrix includes: a wafer layer (111), the wafer layer (111) having cutting lines that are evenly distributed thereon; a first silicon oxide layer (112), the first silicon oxide layer (112) being made of silicon oxide and formed on an upper surface of the wafer layer (111); a transition metal oxide layer (113), the transition metal oxide layer (113) being made of transition metal oxide and formed on an upper surface of the first silicon oxide layer (112). The chip matrix has characteristics such as resistances against high temperature, high humidity and other harsh environments. Meanwhile, by changing pH, surfactant and other components of a solution containing sequences to be sequenced, a surface functional region of the chip matrix can specifically adsorb a sequence to be sequenced.
    Type: Application
    Filed: July 15, 2021
    Publication date: December 9, 2021
    Applicant: BGI SHENZHEN
    Inventors: Shifeng Li, Tengyue Li, Yuan Li, Zhaohui Wang, Xueqin Jiang, Jiacheng Chen, Aoli Wang, Fuxing Huang, Xiaogang Song, Lingling Peng, Handong Li, Wenwei Zhang
  • Publication number: 20210363576
    Abstract: Provided are a nucleic acid sequencing method and a nucleic acid sequencing kit. The kit comprises a nucleic acid probe, a ligase, dNTP having a blocking group attached to a 3? end, a polymerase, a reagent 1 for excising the blocking group attached to the 3? end of the dNTP, and a reagent 2 for excising the remaining nucleotides on the nucleic acid probe that are not bound to a to-be-tested base group.
    Type: Application
    Filed: October 12, 2018
    Publication date: November 25, 2021
    Inventors: Erkai LIU, Wenwei ZHANG, Ao CHEN, Chongjun XU
  • Publication number: 20210355459
    Abstract: Provided are a Phi29 DNA polymerase mutant with improved thermal stability and an use thereof in sequencing. The phi29 DNA polymerase mutant is represented by A) or B) below: the DNA polymerase mutant represented by the A) is a protein having DNA polymerase activity that is obtained by modifying at least one amino acid residue(s) in the following six sites in a phi29 DNA polymerase amino acid sequence: the 97-th, 123-th, 217-th, 224-th, 515-th, and 474-th sites; the DNA polymerase mutant represented by the B) is a protein having DNA polymerase activity that is derived from the A) by adding a tag sequence to a terminal of the amino acid sequence of the protein represented by the A).
    Type: Application
    Filed: October 11, 2018
    Publication date: November 18, 2021
    Inventors: Zhougang ZHANG, Huanhuan LIU, Yue ZHENG, Yujun ZHOU, Jun XIA, Yuliang DONG, Chongjun XU, Wenwei ZHANG
  • Publication number: 20210340509
    Abstract: The present disclosure relates to a reverse transcriptase and an application thereof. The reverse transcriptase has mutation sites such as R450H compared with the wild-type M-MLV reverse transcriptase. The reverse transcriptase has increased polymerase activity, improved thermal stability, and reduced RNase H activity.
    Type: Application
    Filed: June 25, 2021
    Publication date: November 4, 2021
    Inventors: Huanhuan Liu, Na Guo, Huizhen Li, Zhougang Zhang, Hongyan Han, Miaomiao Guo, Yue Zheng, Yuliang Dong, Wenwei Zhang, Chongjun Xu
  • Publication number: 20210324466
    Abstract: Provided is a method for sequencing a long-fragment nucleic acid. The nucleic acid molecules each containing a long insert, a first sequencing adapter, and a second sequencing adapter, is used to construct a sequencing library, and the sequencing is performed in segments to sequence the nucleic acids having the long inserts.
    Type: Application
    Filed: June 4, 2021
    Publication date: October 21, 2021
    Inventors: Lin HE, Sha LIAO, Chongjun XU, Wenwei ZHANG, Ao CHEN
  • Publication number: 20210324353
    Abstract: Provided is a recombinant KOD polymerase, which is the following A) or B): the polymerase shown in A) is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 18 positions in a wild-type KOD DNA polymerase amino acid sequence: 675th, 385th, 710th, 674th, 735th, 736th, 606th, 709th, 347th, 349th, 590th, 676th, 389th, 589th, 680th, 384th, 496th and 383rd; the polymerase described by B) is a protein having DNA polymerase activity that is derived from A) by adding a tag sequence to an end of the amino acid sequence of the protein shown in A).
    Type: Application
    Filed: September 3, 2018
    Publication date: October 21, 2021
    Inventors: Lili ZHAI, Lin WANG, Wenwei ZHANG, Yuliang DONG, Yue ZHENG, Fen LIU
  • Publication number: 20210295117
    Abstract: Printers capable of determining a media type of a media and associated methods are provided. An example method includes scanning a media with a verifier associated with the printer to generate at least two consecutive windows of the media. The example method further includes determining a vertical length of a captured image based on a comparison of image characteristic values captured in the at least two consecutive windows. The example method further includes determining a media characteristic associated with the media based at least on the captured image and the vertical length and one or more stored media profiles. The example method further includes calibrating the printer based on the media characteristic.
    Type: Application
    Filed: June 7, 2021
    Publication date: September 23, 2021
    Applicant: Datamax-O'Neil Corporation
    Inventors: Heng Yew LIM, Ying CAO, Phek Thong LEE, Cheng Khoon NG, WenWei ZHANG
  • Publication number: 20210283285
    Abstract: A germicidal device includes a lighting member operably in an on-state in which the lighting member emits ultraviolet light for killing microorganisms, or in an off-state in which the lighting member emits no light; a sensing member operably detecting at least one event occurred in an area; and a microcontroller unit coupled with the lighting member and the sensing member for controlling operations of the lighting member in a respective state in accordance with the at least one event occurred in the area. The at least one event includes a movement in the area, an acoustic wave in the area, an intrusion into the area, an expiration of an on-state time period in which the lighting member is in the on-state, an expiration of an off-state time period in which the lighting member is in the off-state, and/or an instruction from a remote operation.
    Type: Application
    Filed: January 20, 2021
    Publication date: September 16, 2021
    Inventors: Wei Li, Wenwei Zhang, Wenting Wang, Yuan Di, Annie You
  • Patent number: 11104889
    Abstract: Provided are a group of phi29 DNA polymerase mutants having increased thermal stability and use thereof. The phi29 DNA polymerase mutants are proteins obtained by performing point mutation A and/or point mutation B and/or point mutation C on phi29 DNA polymerase, the point mutation A meaning that an amino acid residue M at position 97 of the phi29 DNA polymerase is mutated to other amino acid residue, the point mutation B meaning that an amino acid residue L at position 123 of the phi29 DNA polymerase is mutated into other amino acid residue, and the point mutation C meaning that an amino acid residue E at position 515 of the phi29 DNA polymerase is mutated to other amino acid residue. The stability of the phi29 DNA polymerase mutants is higher than that of a wild-type phi29 DNA polymerase.
    Type: Grant
    Filed: August 9, 2017
    Date of Patent: August 31, 2021
    Assignee: MGI TECH CO., LTD.
    Inventors: Zhougang Zhang, Huanhuan Liu, Yue Zheng, Yujun Zhou, Xing Liu, Yuliang Dong, Chongjun Xu, Wenwei Zhang
  • Publication number: 20210256330
    Abstract: Printers capable of determining a media type of a media and associated methods are provided. An example method includes scanning a media with a verifier associated with the printer to generate at least two consecutive windows of the media. The example method further includes determining a vertical length of a captured image based on a comparison of image characteristic values captured in the at least two consecutive windows. The example method further includes determining a media characteristic associated with the media based at least on the captured image and the vertical length and one or more stored media profiles. The example method further includes calibrating the printer based on the media characteristic.
    Type: Application
    Filed: February 17, 2020
    Publication date: August 19, 2021
    Applicant: Datamax-O'Neil Corporation
    Inventors: Heng Yew LIM, Ying CAO, Phek Thong LEE, Cheng Khoon NG, WenWei ZHANG
  • Patent number: 11074487
    Abstract: Printers capable of determining a media type of a media and associated methods are provided. An example method includes scanning a media with a verifier associated with the printer to generate at least two consecutive windows of the media. The example method further includes determining a vertical length of a captured image based on a comparison of image characteristic values captured in the at least two consecutive windows. The example method further includes determining a media characteristic associated with the media based at least on the captured image and the vertical length and one or more stored media profiles. The example method further includes calibrating the printer based on the media characteristic.
    Type: Grant
    Filed: February 17, 2020
    Date of Patent: July 27, 2021
    Assignee: DATAMAX-O'NEIL CORPORATION
    Inventors: Heng Yew Lim, Ying Cao, Phek Thong Lee, Cheng Khoon Ng, WenWei Zhang
  • Publication number: 20210214783
    Abstract: A method for constructing a sequencing library, a sequencing library and a sequencing method.
    Type: Application
    Filed: March 25, 2021
    Publication date: July 15, 2021
    Inventors: Luman CUI, Fei FAN, Wenwei ZHANG, Zhou LONG, Weimao WANG, Ou WANG
  • Publication number: 20210172009
    Abstract: Provided are a hooked probe, a method for ligating a nucleic acid and a method for constructing a sequencing library. The hooked probe comprises a target specific area and a hooked area ligated thereto; the target specific area comprises a sequence complementarily paired with at least part of the single chain of the nucleic acid fragment to be ligated; the hooked area comprises a sequence unpaired with the nucleic acid fragment; the end of the hooked area is a ligatable end; and the ligatable end can ligate the end of the single chain of the nucleic acid fragment.
    Type: Application
    Filed: November 9, 2017
    Publication date: June 10, 2021
    Applicant: MGI TECH CO., LTD.
    Inventors: Yuan JIANG, Yang XI, Wenwei ZHANG, Pengjuan LIU, Xia ZHAO, Qiaoling LI, Hanjie SHEN, Yongwei ZHANG, Radoje DRMANAC
  • Publication number: 20210164042
    Abstract: Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.
    Type: Application
    Filed: February 10, 2021
    Publication date: June 3, 2021
    Inventors: Yuan Jiang, Xia Zhao, Qiaoling Li, Shengmao Liu, Bo Wang, Li Chen, Wenwei Zhang, Hui Jiang, Radoje Drmanac
  • Patent number: 10995367
    Abstract: Provided are a vesicular adaptor and a single-chain cyclic library constructed by using the adaptor. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantage of high throughput sequencing, high accuracy and simple operations.
    Type: Grant
    Filed: September 19, 2019
    Date of Patent: May 4, 2021
    Assignee: MGI TECH CO., LTD.
    Inventors: Yuan Jiang, Jing Guo, Xiaojun Ji, Chunyu Geng, Kai Tian, Xia Zhao, Huaiqian Xu, Wenwei Zhang, Hui Jiang, Radoje Drmanac
  • Patent number: 10961519
    Abstract: Provided are a phi29 DNA polymerase mutant with increased thermo stability, a method for preparing the mutant, the use of the mutant, and a method for increasing the stability of the phi29 DNA polymerase.
    Type: Grant
    Filed: October 18, 2019
    Date of Patent: March 30, 2021
    Assignee: MGI TECH CO., LTD.
    Inventors: Huanhuan Liu, Yue Zheng, Yujun Zhou, Xi Zhang, Zhougang Zhang, Yuliang Dong, Wenwei Zhang, Chongjun Xu, Snezana Drmanac