Abstract: Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.

Abstract: An animal trap may include a source of compressed gas; a trigger mechanism configured to be actuated by an animal, and a kill mechanism powered by compressed gas. The trap may include two or more trigger valves connected in series. At least one trigger valve may include an opening, a valve seat surrounding the opening, a valve member having a sealing surface, and an O-ring arranged to seal between the sealing surface and the valve seat. One or more flow paths may allow gas to flow along at least part of an internal periphery of the O-ring. The trigger may include a trigger rod extending in a rest position from a mount at or near a first end thereof. The trigger rod may be mounted for pivoting movement about the mount, and may have two rotational degrees of freedom. Pivoting movement of the trigger rod in any direction away from the rest position may result in actuation of the trigger.

Abstract: An animal trap may include a source of compressed gas; a trigger mechanism configured to be actuated by an animal, and a kill mechanism powered by compressed gas. The trap may include two or more trigger valves connected in series. At least one trigger valve may include an opening, a valve seat surrounding the opening, a valve member having a sealing surface, and an O-ring arranged to seal between the sealing surface and the valve seat. One or more flow paths may allow gas to flow along at least part of an internal periphery of the O-ring. The trigger may include a trigger rod extending in a rest position from a mount at or near a first end thereof. The trigger rod may be mounted for pivoting movement about the mount, and may have two rotational degrees of freedom. Pivoting movement of the trigger rod in any direction away from the rest position may result in actuation of the trigger.

Abstract: Techniques for peer-to-peer Peripheral Component Interconnect Express (PCIe) storage transfers are disclosed. In some embodiments, the techniques may be realized as a method for providing peer-to-peer storage transfers between PCIe devices including providing, in memory of a first PCIe device, a queue for data communicated between the first PCIe device and a target PCIe device, receiving, at the first PCIe device, queue memory allocation information transmitted from a host device communicatively coupled to the first PCIe device and the target PCIe device, and generating, using a computer processor of the first PCIe device, a storage command.

Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.

Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.

Abstract: An animal trap is powered by compressed gas and includes a trigger mechanism and kill mechanism. The trap is preferably self-resetting. A variety of sources of compressed gas may be used, and different kill mechanisms may also be used. The trap is particularly suited to applications where low-maintenance traps are required, because it uses a simple, reliable mechanism which can kill a number of animals over a long period of time without requiring maintenance or manual resetting.

Abstract: This invention relates to the preparation of nucleic acid samples for analysis. The invention may be particularly useful for single stranded samples. Embodiments of the invention involve the attachment of double stranded or hairpin oligonucleotides using template independent polymerase enzymes in the preparation of nucleic acid sequencing libraries.

Abstract: An animal trap includes: a bait, a support, and an elastic band, wherein in a set state the support supports the elastic band in an expanded state, and wherein removal, consumption or destruction of the bait by an animal: causes the elastic band to contract around the animal to kill the animal; or causes the elastic band to drive a kill mechanism to kill the animal. Animal traps may be formed substantially or entirely of biodegradable materials. Animal traps may be adapted for aerial distribution. A trap dispenser may dispense traps as they are triggered.

Abstract: Techniques for peer-to-peer Peripheral Component Interconnect Express (PCIe) storage transfers are disclosed. In some embodiments, the techniques may be realized as a method for providing peer-to-peer storage transfers between PCIe devices including providing, in memory of a first PCIe device, a queue for data communicated between the first PCIe device and a target PCIe device, receiving, at the first PCIe device, queue memory allocation information transmitted from a host device communicatively coupled to the first PCIe device and the target PCIe device, and generating, using a computer processor of the first PCIe device, a storage command.

Abstract: Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.

Abstract: Techniques for peer-to-peer Peripheral Component Interconnect Express (PCIe) storage transfers are disclosed. In some embodiments, the techniques may be realized as a method for providing peer-to-peer storage transfers between PCIe devices including providing, in memory of a first PCIe device, a queue for data communicated between the first PCIe device and a target PCIe device, receiving, at the first PCIe device, queue memory allocation information transmitted from a host device communicatively coupled to the first PCIe device and the target PCIe device, and generating, using a computer processor of the first PCIe device, a storage command.

Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.

Abstract: Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.

Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.

Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.

Abstract: Techniques for peer-to-peer Peripheral Component Interconnect Express (PCIe) storage transfers are disclosed. In some embodiments, the techniques may be realized as a method for providing peer-to-peer storage transfers between PCIe devices including providing, in memory of a first PCIe device, a queue for data communicated between the first PCIe device and a target PCIe device, receiving, at the first PCIe device, queue memory allocation information transmitted from a host device communicatively coupled to the first PCIe device and the target PCIe device, and generating, using a computer processor of the first PCIe device, a storage command.

Abstract: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.

Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.

Abstract: A method for communicating data between peripheral devices and an embedded processor that includes receiving, at a data buffer unit of the embedded processor, the data from a peripheral device. The method also includes copying data from the data buffer unit into the bridge buffer of the embedded processor as a bridge buffer message. Additionally, the method includes creating, after storing the data as a bridge buffer message, a peripheral device message comprising the bridge buffer message, and sending the peripheral device message to a thread message queue of a subscriber.