Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.

Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.

Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.

Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.

Abstract: A method, apparatus, and computer instructions for marking data versioning for code. Responsive to receiving an application programming interface call, a first marking statement in the code and a second marking statement are inserted in the code. A portion of code between the first marking statement and the second marking statement forms a marked section of code. A change made in data in an object caused by execution of the marked portion of code is detected. The detected change is tracked in manner such that the object may be restored to a prior state.

Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.

Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.

Abstract: A method, apparatus, and computer instructions for managing versioning data for memory segments. Responsive to a request from a requester to allocate a memory segment, the memory segment is allocated. A versioning data structure is created for the memory segment. The versioning data structure is used to store changes in data for the memory segment, and the memory segment includes a pointer to the versioning data structure. When a change in the data is detected for the memory segment, wherein the change in the data forms the delta data. Responsive to detecting the change in the data for the memory segment, the delta data is stored in the versioning data structure, wherein the versioning data structure is used to return the data in the memory segment to a prior state.

Abstract: A marking device is disclosed to enable the placement of an indicator mark on one side of a sheet of material to mark the approximate periphery of a stationary object. The device consists of a base containing multiple marking members with a marking surface raised above the base surface. A contact plate, portions of which extend above the base surface, is connected to the base through the use of a pair of opposing connectors. The contact plate extends from the base a distance sufficient to prevent the sheet material from contacting the marking members without the application of sufficient pressure to compress the contact plate.

Abstract: The invention discloses an apparatus and method including a bed and a therapeutic mattress having a cover therefor. The bed has a frame and a therapeutic mattress with a plurality of inflatable air chambers; a cover on the mattress has a generally planar extent sufficient for covering at least a portion of the mattress; a plurality of spaced apart bolsters extend upwardly from the top surface of the cover adjustably connected and oriented generally parallel to a periphery of the mattress; a plurality of connectors is positioned on the cover and are engageable with the mattress and/or the bed frame to thereon secure the cover; the plurality of bolsters includes at least two bolsters sufficiently spaced apart along a lateral periphery of the mattress to therebetween leave a gap to aid ingress and egress of a person, and at least one bolster positioned along the foot of the mattress.

Abstract: A marking device is disclosed to enable the placement of an indicator mark on one side of a sheet of material to mark the approximate periphery of a stationary object. The device consists of a base containing at least one inking transfer with a marking surface raised above the base surface. Multiple flexible self-hinges, each having a width, a thickness, a first end and a second end extend, from the first end, at an angle from the first surface of the base. Each of the self-hinges as a contact pad, positioned parallel to the base and adjacent to the second end of the self-hinge. The self-hinges extend from the base a distance sufficient to prevent the sheet material from contacting the inking transfer without the application of sufficient pressure to compress the self-hinges. In one embodiment, the base contains positioned to receive the self-hinges and contact pads during compression. The second surface of the base has affixing members to enable the base to be positioned on the item to be marked.

Abstract: A marking device is disclosed to enable the placement of an indicator mark on one side of a sheet of material to mark the approximate periphery of a stationary object. The device consists of a base containing at least one inking transfer with a marking surface raised above the base surface. Multiple flexible self-hinges, each having a width, a thickness, a first end and a second end extend, from the first end, at an angle from the first surface of the base. Each of the self-hinges as a contact pad, positioned parallel to the base and adjacent to the second end of the self-hinge. The self-hinges extend from the base a distance sufficient to prevent the sheet material from contacting the inking transfer without the application of sufficient pressure to compress the self-hinges. In one embodiment, the base contains positioned to receive the self-hinges and contact pads during compression. The second surface of the base has affixing members to enable the base to be positioned on the item to be marked.

Abstract: A park system alarm is provided which is energized when operation of the park mechanism fails to open the park system switch, even though the shift lever is in the park position, and the driver initiates the process of opening his or her door. An external alarm is also energized through the park system switch and a seat switch if the driver leaves his normal driving position when the shift lever is not in park and when the shift lever is in park but the park system is broken or maladjusted. A put-in-park alarm is energized through the park system switch, a gear switch, and a door switch if the driver initiates the process of opening his or her door with the shift lever in any position except park. A seat switch circuit causes the parking pawl to engage the parking gear if the driver leaves the vehicle without placing the shift lever in park.

Abstract: An easy to use and accurate marking device enables the placement of an indicator mark on one side of a sheet of material to mark the periphery of an object. A marking line located within the base transfers a marking substance to the material upon contact. A recess within the base contains a contact pad that is moveable within the recess. The pad is maintained a predetermined distance from the base plate, when not under pressure, by a spring between the contact pad and the recess. At least one retaining member prevents removal of the contact pad from the base plate while permitting contact pad movement within the recess. At least one holding member, such as flanges forming a friction fit, is used to secure the base plate to the stationary object. A portion of the body of the marking device can be notched, giving a thinner cross-sectional thickness and placing at least a portion of the recess into the object.

Abstract: A park system alarm is provided which is energized when operation of the park mechanism fails to open the park system switch, even though the shift lever is in the park position, and the driver initiates the process of opening his or her door. An external alarm is also energized through the park system switch and a seat switch if the driver leaves his normal driving position when the shift lever is not in park and when the shift lever is in park but the park system is broken or maladjusted. A put-in-park alarm is energized through the park system switch, a gear switch, and a door switch if the driver initiates the process of opening his or her door with the shift lever in any position except park.

Abstract: A put-in-park alarm which is energized whenever an automatic shift vehicle is stopped but the shift lever is not in park. The put-in-park alarm is energized when the driver moves his door handle in a door opening direction. Not only is the alarm energized but the door cannot be opened until the shift lever is moved to park whereupon the signal is deenergized, but the door is unlocked upon a second movement of the handle in a door opening direction. The invention includes a broken/maladjusted alarm which is different from the put-in-park alarm but produces substantially similar results.

Abstract: A vehicle seat back cushion assembly includes a substantially U-shaped outer cushion with a removable cushion insert positioned within the central opening formed by the substantially U-shaped outer cushion. The removable cushion insert cooperates with the outer cushion to form an aperture therebetween. Plastic strips are provided on the outer cover and cushion insert such that at least one Christmas tree-type fastener may be inserted through the plastic strips to attach the removable insert and outer cushion to the seat back frame. The cushion insert is also zippered to the outer cushion.

Abstract: A put-in-park alarm which is energized whenever an automatic shift vehicle is stopped but the shift lever is not in park. The put-in-park alarm is energized when the driver moves his door handle in a door opening direction. Not only is the alarm energized but the door cannot be opened until the shift lever is moved to park whereupon the signal is deenergized, but the door is unlocked upon a second movement of the handle in a door opening direction. The invention includes a broken/maladjusted alarm which is different from the put-in-park alarm but produces substantially similar results.

Abstract: A vehicle seat assembly comprises a lower support frame with a seat back frame pivotally connected to the lower support frame. The back frame includes a headrest portion with an aperture formed therethrough. First and second recliner brackets are secured to the lower support frame between the lower support frame and seat back frame, and each recliner bracket forms a slot therein. A seat dump mechanism includes a lever extending from the aperture with first and second cables operatively connected to the lever. First and second lock-pins are operatively connected to the cable and selectively movable between the first position in which the first and the second lock-pins are engaged within the first and second slots, and a second position in which the first and second lock-pins are disengaged from the first and second slots such that the back frame is disengaged from the recliner to allow pivotal movement of the back frame with respect to the lower support frame.

Abstract: A put-in-park alarm which is energized whenever an automatic shift vehicle is stopped but the shift lever is not in park. The put-in-park alarm is energized when the driver moves his door handle in a door opening direction. Not only is the alarm energized but the door cannot be opened until the shift lever is moved to park whereupon the signal is deenergized, but the door is unlocked upon a second movement of the handle in a door opening direction. The invention includes a broken/maladjusted alarm which is different from the put-in-park alarm but produces substantially similar results.