Abstract: The present disclosure relates to pharmaceutical compounds, compositions and methods, especially as they are related to compositions and methods comprising a compound of any one of Formulas (I), (II), (III), and (IV) as further described herein. The compounds are inhibitors of protein kinases associated with cancers, and are thus useful for the treatment and/or prevention of a tumor or cancer.

Abstract: Provided herein is a pleuromutilin derivative containing a cycloalkyl group and preparation method and application thereof. Specifically, the pleuromutilin is reacted with p-toluenesulfonyl chloride to obtain an intermediate I, and the intermediate I is reacted with 1-amino-2-methylpropane-2-thiol hydrochloride to obtain an intermediate II, and then the intermediate II is reacted with cycloalkylcarbonyl chloride to obtain a target derivative. The synthesis process of these derivatives is simple, with a high yield and simple purification. These derivatives can effectively inhibit activities of Staphylococcus aureus and Streptococcus, and have superior activities to the marketed Tiamulin, and they also have good antibacterial activities against drug-resistant bacteria. In particular, the treatment effect of the derivative 2 of the target derivative is significantly superior to the clinical drugs Tiamulin and Valnemulin.

Abstract: Provided herein, inter alia, are compounds, pharmaceutical compositions and methods related to the treatment of viral infections caused by coronavirus or enterovirus. Provided herein are compounds of Formula (I), (II) and (III) and methods of using the compounds for therapy. These compounds are peptidomimetics that inhibit protease 3CL of a coronavirus, and are useful for treating conditions caused by viral infections, including COVID-19.

Abstract: A production method for a bispecific antibody, comprising constructing cells for producing a bispecific antibody, screening the cells, culturing the cells obtained in step one to obtain a culture solution, and performing separation and purification to obtain the bispecific antibody. The cells comprise CHO cells. The culture mode comprises fed-batch culture or perfusion culture. Various influence factors in fermentation culture and separation and purification are comprehensively analyzed, and by effective control, all factors can effectively cooperate, so that a high-purity bispecific antibody is efficiently produced, the daily output can reach 3 g/L or above, and the purity of the purified bispecific antibody can reach 90% or above. Moreover, the production process is amplified and verified, the process is stable and reliable, the cost is low, a great breakthrough is achieved compared with an existing production method.

Abstract: The present disclosure provides an information processing method and apparatus, an electronic device, and a storage medium. The present disclosure provides an information processing method. The method includes: receiving a first document, so as to perform preset processing on the first document; determining, based on document content of the first document, or the document content and associated information of the first document, whether there is an alert item in the first document that satisfies a preset condition; and displaying the first document in association with the alert item in response to there being the alert item in the first document, where the alert item is displayed in association with category information.

Abstract: A fused ring-containing compound, an application thereof, and a composition containing same, and specifically, a fused ring-containing compound represented by formula II, a pharmaceutically acceptable salt or solvate thereof, or a solvate of said pharmaceutically acceptable salt. The compound has good STAT5 inhibitory activity.

Abstract: Provided is a perception evaluation method for a bearing capacity of a highway tunnel lining structure, including: from the load effect evolution state of surrounding rock and the decay mechanical state of a lining structure, the characterization index system of a highway tunnel bearing capacity is constructed; a characterization index inversion function relationship based on strain data of an inner surface of the highway tunnel lining is provided, a bearing capacity perception layout scheme of the tunnel structure is constructed accordingly, and a complete set of perception method for bearing capacity characterization indexes is obtained; then, by calculating a load effect function and a resistance effect function, the calculation and evaluation method of the tunnel structure bearing capacity.

Abstract: Methods for managing a robot arm are disclosed. In one method, during a movement of the robot arm in an axis thereof, a signal collected by a sensor equipped at a frame of the robot arm is received. A change in strength of the received signal is detected, where the change is caused by an offset between a position of a reference mark equipped at the robot arm and a position of the sensor. An original point of the axis of the robot arm is determined based on the detected change. Further, robot systems, apparatuses, systems, and computer readable media for managing a robot arm in the robot systems are disclosed. The original point of the axis of the robot arm may be determined without a dedicated calibrating tool, and then the robot arm may be calibrated based on the original point accurately.

Abstract: Provided are a cardiomyocyte preparation, a preparation method therefor and the use of the preparation in treating heart failure. The cardiomyocyte preparation comprises cardiomyocytes and fibroblasts, differentiated from pluripotent stem cells, wherein the concentration of the cardiomyocytes in the cardiomyocyte preparation is 0.75×107-1.0×109 cells/mL, and the content of the cardiomyocytes is higher than 80% while the content of the fibroblasts is not higher than 20%. The cardiomyocyte preparation can improve cardiac ejection fraction and left ventricular fractional shortening, and increase the thickness of the left ventricular inner wall.

Abstract: A Co-based superelastic alloy material contains a Co—Cr—Al—Si-based alloy and has a composition (at. %) within a pentagon area on the Co—Cr—(Al,Si) pseudo-ternary phase diagram, and containing Al: 2.5 at. % to 10.0 at. % and Si: 2.5 at. % to 13.0 at. %, the pentagon area being formed of five points of point A: Co49.5Cr27.5(Al,Si)23, point B: Co57Cr27.5(Al,Si)13.5, point C: Co50Cr45(Al,Si)5, point D: Co45Cr45(Al,Si)10, and point E: Co45Cr32(Al,Si)23.

Abstract: Methods of assessing cytolysis of cancer cells are provided, which include: providing a cell-substrate impedance monitoring device operably connected to an impedance analyzer, adding target cells characterized as cancer cells to a well of the device adding effector cells to the well to form a test well, where the effector cells are immune cells obtained or derived from a same patient as the target cells; monitoring cell-substrate impedance of the test well before and after adding the effector cells and optionally deriving an impedance-based parameter from the impedance; and determining effectiveness of effector cell killing of the target cells by comparing the impedance or impedance-based curves over time.

Abstract: A battery includes multiple battery cells, each battery cell includes shell, electrode terminal, and insulation layer, the shell includes a first wall, which includes a body and a first convex part, the body has an inner surface and an outer surface arranged oppositely in the thickness direction thereof, the first convex part protrudes from the outer surface in a direction away from the inner part of the battery cells, the electrode terminal is arranged on the body, and the insulation layer covers and is fixed on the first convex part; and a box, provided with an accommodating cavity with an opening, wherein the multiple battery cells are accommodated in the accommodating cavity, and the insulation layer binds with the cover piece covering the opening to fixedly connect the multiple battery cells to the cover piece.

Abstract: A system for monitoring cells, which includes a device for monitoring cell-substrate impedance, the device having a plurality of wells on a nonconductive substrate, where each of the plurality of wells has an electrode array fabricated on the substrate for measurement of cell-substrate impedance; an impedance analyzer that measures cell-substrate impedance from the plurality of wells; electronic circuitry with multiple analogue-to-digital conversion channels, where the electronic circuitry electrically connects the electrode arrays to the impedance analyzer such that the electrode arrays are electrically monitored at millisecond time resolution; and a software program that analyzes the measured cell-substrate impedance.

Abstract: Provided herein, inter alia, are compounds, pharmaceutical compositions and methods related to the treatment of viral infections caused by coronavirus or enterovirus. Provided herein are compounds of Formula (I), (II) and (III) and methods of using the compounds for therapy. These compounds are peptidomimetics that inhibit protease 3CL of a coronavirus, and are useful for treating conditions caused by viral infections, including COVID-19.

Abstract: A plurality of constraint relationships in a UI are identified and grouped into two types, a constraint relationship of a first type is solved in an integrated development environment (IDE), and a constraint relationship of a second type is a constraint relationship solved in a terminal or the IDE. The plurality of constraint relationships are used to determine a layout of one or more components in the UI. The IDE calculates a first intermediate solution set based on the constraint relationship of the first type in the plurality of constraint relationships. The first intermediate solution set is used to determine the layout of the one or more components in the UI together with the constraint relationship of the second type.

Abstract: A defect detection method includes obtaining a first screen image and a second screen image of a screen module, in response to determining a defect area in the first screen image, determining an image feature correlation between the first screen image and the second screen image, and determining a defect detection result of the screen module based on the image feature correlation. The first screen image is an image displayed by a display screen collected when the display screen of the screen module is in a first test state. The second screen image is an image displayed by the display screen collected when the display screen is in a second test state. The defect area indicates that the screen module has a display defect.

Abstract: A system for monitoring cells, which includes a device for monitoring cell-substrate impedance, the device having a plurality of wells on a nonconductive substrate, where each of the plurality of wells has an electrode array fabricated on the substrate for measurement of cell-substrate impedance; an impedance analyzer that measures cell-substrate impedance from the plurality of wells; electronic circuitry with multiple analogue-to-digital conversion channels, where the electronic circuitry electrically connects the electrode arrays to the impedance analyzer such that the electrode arrays are electrically monitored at millisecond time resolution; and a software program that analyzes the measured cell-substrate impedance.

Abstract: This disclosure relates, inter alia to: modified interleukin 2 (IL-2) polypeptides; RNA polynucleotides, DNA polynucleotides, non-viral vectors, and viral vectors encoding such modified IL-2 polypeptides; methods of making such modified IL-2 polypeptides and RNA polynucleotides, DNA polynucleotides, non-viral vectors, and viral vectors encoding same; and methods of using such modified IL-2 polypeptides and RNA polynucleotides, DNA polynucleotides, non-viral vectors, and viral vectors encoding same.

Abstract: The present disclosure provides a method for knocking out an N-myristoyltransferase (NMT) gene from Eimeria tenella, and belongs to the technical field of microorganisms. The method includes: mixing sporozoites of Eimeria tenella with a pCRISPR::EtNMT plasmid and a pEtNMT::DHFR plasmid, and subjecting a resulting mixture to electrotransformation to obtain NMT gene-knockout Eimeria tenella. The method provided by the present disclosure can successfully knock out the NMT gene from Eimeria tenella, which lays a foundation for studying the function of the Eimeria tenella gene and developing a vaccine therefor.

Abstract: An engineered radioactive polymeric microsphere, and a preparation and application thereof. The preparation method includes: adding a styrene monomer and a disperser and/or a crosslinker into a medium, followed by feeding of nitrogen or helium and stirring to obtain a first reaction mixture; heating the first reaction mixture, and adding an initiator, followed by reaction under stirring at a constant temperature to obtain a second reaction mixture; subjecting the second reaction mixture to washing with ethanol and water, and vacuum drying to obtain a crude polymeric microsphere; subjecting the crude polymeric microsphere to radiation-induced graft polymerization with a functional monomer to obtain the functionalized polymeric microsphere; and exposing the functionalized polymeric microsphere to a radionuclide to prepare the engineered radioactive polymeric microsphere.