Abstract: Embodiments of the invention are directed to stable full-length cDNA clones of a clinical, Asian lineage ZIKV strain. Certain embodiments of the invention are directed to high-throughput assays for ZIKV and dengue virus (DENV) diagnosis.

Abstract: The invention generally relates to the development of variant Zika strains, cDNA clones and mRNA transcripts that contain specific mutations in the Zika ORF, and methods for producing high yields of Zika viruses (“ZIKVs”) using these variant cDNA clones, transcripts and strains. The produced ZIKVs can be used for the manufacture of purified inactivated vaccines (PIVs), which may be useful for treating ZIKV-related diseases and for providing immunoprotection against ZIKV.

Abstract: The invention generally relates to the development of variant Zika strains, cDNA clones and mRNA transcripts that contain specific mutations in the Zika ORF, and methods for producing high yields of Zika viruses (“ZIKVs”) using these variant cDNA clones, transcripts and strains. The produced ZIKVs can be used for the manufacture of purified inactivated vaccines (PIVs), which may be useful for treating ZIKV-related diseases and for providing immunoprotection against ZIKV.

Abstract: A method for controlling an inverter including, when it is detected that a voltage across a bus capacitor, for example, a bus voltage, is excessively large, controlling a photo-voltaic (PV) battery to short-circuit so that the bus voltage drops. When the bus voltage drops to a particular value, continuing charging, by the PV battery, the bus capacitor to control the bus voltage to rise so that the bus voltage is kept within an operating voltage range of the inverter and the inverter can be equipped with a PV battery having a higher open circuit voltage. Therefore, a charging rate of the bus capacitor can be increased and a startup speed of the inverter can be increased.

Abstract: Provided is a method of detecting the presence of an anti-Zika virus (ZIKV) antibody in a sample, including contacting a sample with a suspension having a plurality of microspheres wherein individual microspheres are conjugated to a peptide and the peptide includes a ZIKV peptide selected from the group including ZIKV NS1, ZIKV NS5, and ZIKV envelope protein, forming a first incubated suspension by incubating said sample with said suspension to permit binding of anti-ZIKV antibodies present in the sample to said microspheres, forming a second incubated suspension by contacting said first incubated suspension with an anti-ZIKV antibody detecting-reagent to permit binding of the anti-ZIKV antibody detecting reagent to said microspheres, removing from the second incubated suspension anti-ZIKV antibody detecting-reagent molecules that are not bound to said microspheres, and detecting the presence of anti-ZIKV antibody detecting-reagent molecules in the second incubated suspension.

Abstract: A method for controlling an inverter including, when it is detected that a voltage across a bus capacitor, for example, a bus voltage, is excessively large, controlling a photo-voltaic (PV) battery to short-circuit so that the bus voltage drops. When the bus voltage drops to a particular value, continuing charging, by the PV battery, the bus capacitor to control the bus voltage to rise so that the bus voltage is kept within an operating voltage range of the inverter and the inverter can be equipped with a PV battery having a higher open circuit voltage. Therefore, a charging rate of the bus capacitor can be increased and a startup speed of the inverter can be increased.

Abstract: The present invention discloses a method of eliciting an immune response and a method of vaccination comprising administration of a mutated flavivirus. The mutated flavivirus comprises at least one mutation in a nucleic acid sequence encoding for the non-structural protein 5 of the flavivirus sequence resulting in inactivation of the 2?O-methyltransferase.

Abstract: A method for backing up a TCP connection includes a data transmission process and a data receiving process. The data transmission process includes obtaining, by an AMB of a transmitting end, boundary information of data; and backing up the data and the boundary information of the data to a SMB of the transmitting end. The data receiving process includes backing up, by the SMB, data received from a peer side of the TCP connection and the boundary information of the data received by the peer side to the AMB; and deleting the data received by the peer side from the data backed up by the SMB during the transmission process according to the boundary information of the data received by the peer side. The disclosure also provides an apparatus for backing up a TCP connection.

Abstract: A method and a system for process upgrade are disclosed. A source process implements data interaction, via a socket interface, with a far end entity connected with the socket interface. When the source process intends to upgrade itself, the source process copies its process data to a target process and determines if the data from the far end entity received via socket interface is complete. If it is determined that the data is complete, the source process ceases data interaction with the socket interface and copies the received data to the target process. After the data is copied, the source process sends an upgrade and switch indication to the target process and enables the target process to send an upgrade and switch request to the socket interface. The socket interface then switches a socket interface service to the target process. Accordingly, the technical solutions according to the present invention realize a reliable and smooth process upgrade without disconnecting the socket connection.

Abstract: Piezoelectric nanostructures, including nanofibers, nanotubes, nanojunctions and nanotrees, may be made of piezoelectric materials alone, or as composites of piezoelectric materials and electrically-conductive materials. Homogeneous or composite nanofibers and nanotubes may be fabricated by electrospinning. Homogeneous or composite nanotubes, nanojunctions and nanotrees may be fabricated by template-assisted processes in which colloidal suspensions and/or modified sol-gels of the desired materials are deposited sequentially into the pores of a template. The electrospinning or template-assisted fabrication methods may employ a modified sol-gel process for obtaining a perovskite phase in the piezoelectric material at a low annealing temperature.

Abstract: Piezoelectric nanostructures, including nanofibers, nanotubes, nanojunctions and nanotrees, may be made of piezoelectric materials alone, or as composites of piezoelectric materials and electrically-conductive materials. Homogeneous or composite nanofibers and nanotubes may be fabricated by electrospinning. Homogeneous or composite nanotubes, nanojunctions and nanotrees may be fabricated by template-assisted processes in which colloidal suspensions and/or modified sol-gels of the desired materials are deposited sequentially into the pores of a template. The electrospinning or template-assisted fabrication methods may employ a modified sol-gel process for obtaining a perovskite phase in the piezoelectric material at a low annealing temperature.

Abstract: The present invention relates to anti-flavivirus compounds, including lycorine and derivatives thereof, and their use in treating a subject infected by a flavivirus. The present invention also relates to the use of the anti-flavivirus compounds for the prophylaxis of flavivirus infection. The present invention further relates to a method of suppressing viral RNA synthesis of a flavivirus. Also described is a method of preparing an anti-flavivirus compound for use in the treatment or prophylaxis of flavivirus infection.

Abstract: Piezoelectric nanostructures, including nanofibers, nanotubes, nanojunctions and nanotrees, may be made of piezoelectric materials alone, or as composites of piezoelectric materials and electrically-conductive materials. Homogeneous or composite nanofibers and nanotubes may be fabricated by electrospinning. Homogeneous or composite nanotubes, nanojunctions and nanotrees may be fabricated by template-assisted processes in which colloidal suspensions and/or modified sol-gels of the desired materials are deposited sequentially into the pores of a template. The electrospinning or template-assisted fabrication methods may employ a modified sol-gel process for obtaining a perovskite phase in the piezoelectric material at a low annealing temperature.

Abstract: The instant application provides a fluidic array device having an elastomeric body that provides easy fluidic control of the device. The elastomeric body may include plurality of intersecting row and column channels. Reactions may occur at the intersection spots formed by the intersecting channels. Pinching applied at suitable locations along the channels enables the channels to be opened or closed, and thus provides control of fluids pumped through the device. The surfaces of the channels and intersection spots may be engineered to have certain properties. In particular, the intersection spots may be nanoengineered to have surface properties differing from those of the channels, and thus reactions may be selectively controlled to occur only, or highly preferentially, in the intersection spots.

Abstract: The present invention provides a rapid and sensitive method for the detection of a West Nile virus (WNV), Japanese encephalitis virus (JEV), St. Louis encephalitis virus (SLEV) and Dengue virus (DENV) and antibodies directed against thereof involving contacting a biological specimen suspected of being infected with WNV, JE, SLE or DEN with a substantially purified and isolated WNV E glycoprotein or subfragment thereof having a native conformation wherein the E glycoprotein or subfragment thereof has a reactivity with antibodies against WNV and a cross-reactivity with antibodies against JEV, SLEV and DENV. The instant invention further provides a rapid, sensitive, and consistent method for the specific detection of WNV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-WNV antibodies but not cross-reactive with antibodies against other flaviviruses such as JEV, SLEV, or DENV.

Abstract: A method of fabricating micro- and nano-scale fiber comprises: spreading micro- and nano-scale particles into a liquid or fluid-like material prior to forcing portions of the liquid or fluid-like material that surround the particles to depart from the original liquid or fluid-like environment by using a force field; stretching to elongate the portions of the liquid or fluid-like material until the free ends of the stretched portions stop motion to complete fiber or fiber-like structures in micro- and nano-scales.

Abstract: A method and a system for process upgrade are disclosed. A source process implements data interaction, via a socket interface, with a far end entity connected with the socket interface. When the source process intends to upgrade itself, the source process copies its process data to a target process and determines if the data from the far end entity received via socket interface is complete. If it is determined that the data is complete, the source process ceases data interaction with the socket interface and copies the received data to the target process. After the data is copied, the source process sends an upgrade and switch indication to the target process and enables the target process to send an upgrade and switch request to the socket interface. The socket interface then switches a socket interface service to the target process. Accordingly, the technical solutions according to the present invention realize a reliable and smooth process upgrade without disconnecting the socket connection.

Abstract: Piezoelectric nanostructures, including nanofibers, nanotubes, nanojunctions and nanotrees, may be made of piezoelectric materials alone, or as composites of piezoelectric materials and electrically-conductive materials. Homogeneous or composite nanofibers and nanotubes may be fabricated by electrospinning. Homogeneous or composite nanotubes, nanojunctions and nanotrees may be fabricated by template-assisted processes in which colloidal suspensions and/or modified sol-gels of the desired materials are deposited sequentially into the pores of a template. The electrospinning or template-assisted fabrication methods may employ a modified sol-gel process for obtaining a perovskite phase in the piezoelectric material at a low annealing temperature.

Abstract: A method for backing up a TCP connection includes a data transmission process and a data receiving process. The data transmission process includes obtaining, by an AMB of a transmitting end, boundary information of data; and backing up the data and the boundary information of the data to a SMB of the transmitting end. The data receiving process includes backing up, by the SMB, data received from a peer side of the TCP connection and the boundary information of the data received by the peer side to the AMB; and deleting the data received by the peer side from the data backed up by the SMB during the transmission process according to the boundary information of the data received by the peer side. The disclosure also provides an apparatus for backing up a TCP connection.

Abstract: The instant application provides a fluidic array device having an elastomeric body that provides easy fluidic control of the device. The elastomeric body may include plurality of intersecting row and column channels. Reactions may occur at the intersection spots formed by the intersecting channels. Pinching applied at suitable locations along the channels enables the channels to be opened or closed, and thus provides control of fluids pumped through the device. The surfaces of the channels and intersection spots may be engineered to have certain properties. In particular, the intersection spots may be nanoengineered to have surface properties differing from those of the channels, and thus reactions may be selectively controlled to occur only, or highly preferentially, in the intersection spots.