CROSS REFERENCE TO RELATED APPLICATIONS This application is related to and claims the benefit of priority of U.S. Provisional Application Ser. No. 61/266,733, filed Dec. 4, 2009, which is incorporated herein by, reference.
STATEMENT OF GOVERNMENT SUPPORT This invention was made with United States government support from the National Institutes of Health grant no. K12-CA-100639. The United States government has certain rights in this invention.
SEQUENCE LISTING The sequence listing is filed with the application in electronic format only and is incorporated by reference herein. The sequence listing text file “WO_ASFILED_SequenceListing.txt” was created on Dec. 3, 2010, and is 262,254 bytes in size.
BACKGROUND Naive B cells migrate through the circulation to lymphoid organs where they undergo the T cell-dependent germinal center reaction. Adaptive immunity is acquired as specific antigen-reactive germinal center B cells differentiate into the major effector B cells of the adaptive immune system: memory cells and plasma cells. See, e.g., FIG. 1A. Although the role of specific transcription factors in mature B cell differentiation has been examined (see Nutt et al. Nature. 1999; 401:556-562; Chang et al. Proc Natl Acad Sci USA. 1996; 93:6947-6952; Turner et al. Cell. 1994; 77:297-306; Shaffer et al., Immunity. 2004; 21:81-93; and Schebesta et al. Curr Opin Immunol. 2002; 14:216-223), mechanisms regulating such transcription factors during mature B cell differentiation are largely unknown.
Many malignancies derived from mature B cells are known and are believed to constitute the majority of leukemias and lymphomas. Such malignancies appear to reflect defined stages of normal B cell differentiation. Diagnosis of leukemias and lymphomas can be particularly difficult because of their shared lineage. These cancers frequently display overlapping morphologies, genetic abnormalities, and expression of surface markers, which can complicate the diagnosis. However, the distinction of these tumors is clinically important because there are important differences in the treatments and expected response to treatment. Thus methods that improve the accuracy of their diagnosis should provide to improved outcomes for these patients.
MicroRNAs are commonly 18-22 nucleotide-long RNA molecules that regulate expression of genes. There is an increasing recognition of the role of microRNAs in oncogenesis, lineage-selection, and immune cell function, including early B cell differentiation. See Calin et al. N Engl J Med. 2005; 353:1793-1801; O'Donnell et al. Nature. 2005; 435:839-843; Chen et al. Science. 2004; 303:83-86; Lim et al. Nature. 2005; 433:769-773; Li et al. Cell. 2007; 129:147-161; Xiao et al., Nat Immunol. 2008; 9:405-414; Baltimore et al. Nat Immunol. 2008; 9:839-845; and Ventura et al. Cell. 2008; 132:875-886. However, the full extent and function of microRNA expression during mature B cell differentiation and in B cell malignancies are not known.
Correct diagnosis of B cell malignancies is important from both a clinical standpoint and from the standpoint of setting appropriate patient expectations. A misdiagnosed B cell malignancy may lead to an inappropriate therapy, which can unnecessarily endanger the patient's life and/or be an ineffective treatment for the B cell malignancy. As an example, the diagnostic distinction of Burkitt lymphoma (BL) from diffuse large B cell lymphomas (DLBCLs) can be difficult because of overlapping morphology, immunophenotype and cytogenetics. Burkitt lymphoma tumors are molecularly distinct from DLBCL, however. The difficulty and importance of obtaining the correct diagnosis in BL was highlighted by the experience of a multicenter clinical trial, CALGB trial#925119, in which nearly half of the 100 patients with an assigned diagnosis of BL were found to have another diagnosis upon further pathology review.
If diagnosed and treated appropriately, nearly 80% of patients with BL can be cured with intensive (high dose) chemotherapy regimens. Thus, a misclassification of BL as DLBCL can result in a missed opportunity to cure the malignancy. On the other hand, misclassification of DLBCL as BL leads to unnecessarily morbidity from intensive chemotherapy regimens. Thus, methods that improve the diagnosis of BL, and other B cell malignancies, can provide better outcomes in patients.
DLBCLs can also be subclassified into two different B cell malignancies, activated B-cell (ABC) DLBCL and germinal center B cell like (GCB) DLBCL. There are at least two important clinical applications for the molecular sub-grouping of DLBCL patients. First, the prognostic information could inform the choices and expectations of patients and their physicians. Second, the important molecular differences in these subgroups form the basis of testing different targeted therapies in these patients. The possibility of a differential response to therapy among ABC and GCB DLBCLs is supported by data that suggest that the benefit of receiving a proteosome inhibitor, bortozemib, is predominantly limited to those patients who have ABC DLBCL. However, the clinical distinction of the subgroups of DLBCL using immunohistochemistry is difficult with current methods distinguishing GCB DLBCL from non-GCB DLBCL with limited success.
Mature B cell differentiation is important for the development of adaptive immunity. The process is also of interest because B cell malignancies are common and retain a number of features derived from their normal counterpart B cell subsets. Unlike other maturation pathways in the hematopoietic and other cell lineages, successive stages of mature B cells do not simply signify progressive differentiation away from the stem cell stage. Rather, each stage represents a specialized state with specific functions. Thus, germinal center (GC) cells interact with CD4 T cells and dendritic cells and undergo somatic hypermutation and Ig-heavy chain class-switching. On the other hand, plasma cells secrete immunoglobulin, while memory cells are primed to proliferate and differentiate into plasma cells upon repeat contact with antigen. The specialized functions demand a finely tuned program of gene regulation.
MicroRNAs represent a novel class of biomarkers that provide new opportunities for clinical translation. First, intact microRNAs can be isolated from tissues preserved using standard methods, such as formalin fixed, paraffin embedded (FFPE) tissue. Thus, microRNA-based biomarkers could be easy to translate to clinical use. Second, microRNAs can be readily assayed using real-time PCR and other methods available in conventional pathology.
SUMMARY In an aspect the disclosure provides an isolated nucleic acid molecule having at least 80% sequence identity to any one of SEQ ID NOs: 763-1350 or 1565 or a complementary sequence thereof. Embodiments provide for sequence identity of at least 90% or 95%.
In an aspect the disclosure provides an isolated nucleic acid molecule comprises any one of SEQ ID NOs: 763-1350 or 1565 or a complementary sequence thereof. Embodiments provide for isolated nucleic acid molecules comprising a primary miRNA, a precursor miRNA, a mature miRNA, or a DNA molecule coding therefore. Embodiments further provide for a cDNA molecule comprising sequence that corresponds to a miRNA sequence of any one of SEQ ID NOs: 763-1350 or 1565.
Aspects of the disclosure provide compositions, pharmaceutical compositions, vectors, host cells, and DNA libraries comprising at least one nucleic acid molecule described herein.
In an aspect the disclosure provides a method of determining the B cell stage of a B cell malignancy in a subject comprising determining the level of expression of at least one microRNA in a sample comprising a B cell taken from the subject relative the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in Table 4.
In an aspect the disclosure provides a method of identifying a B cell malignancy in a subject comprising determining the level of expression of at least one microRNA in a sample comprising a B cell taken from the subject relative the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in Table 4.
In an aspect the disclosure provides a method of diagnosing a B cell malignancy in a subject comprising determining the level of expression of at least one microRNA in a sample comprising a B cell taken from the subject relative the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in Table 4.
In an aspect the disclosure provides a method of identifying a B cell malignancy in a subject comprising determining the level of expression of at least one microRNA in a sample comprising a B cell taken from the subject relative the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in any one of Tables 7 to 35.
In an aspect the disclosure provides a method of diagnosing a B cell malignancy in a subject comprising determining the level of expression of at least one microRNA in a sample comprising a B cell taken from the subject relative to the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in any one of Tables 7 to 35.
Embodiments of these aspects provide for identification or diagnosis of a B cell malignancy selected from chronic lymphocytic leukemia, follicular lymphoma, Hodgkin's lymphoma, activated B-cell diffuse large B cell lymphoma (DLBCL), germinal center-like DLBCL, and Burkitt lymphoma.
In an aspect the disclosure provides a method of determining whether a B cell malignancy in a sample is Burkitt lymphoma, activated B cell-like diffuse large B cell lymphoma (DLBCL), or germinal center-like DLBCL, comprising determining the level of expression of at least one microRNA in the sample relative to the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in Table 10, column “BL miRNA list”; Table 14, column “BL High”; Table 11; Table 14, column “ABC High”; Table 10, column “GCB miRNA list”; Table 14, column “GCB High”; Table 32, or Table 35.
In an aspect the disclosure provides a method of determining whether a B cell malignancy in a sample is Burkitt lymphoma, activated B cell-like diffuse large B cell lymphoma (DLBCL), or germinal center-like DLBCL, comprising determining the level of expression of at least one microRNA in the sample relative to the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in at least one column labeled “GCBvsBL” or “GCBvsABC” in Table 7 or “ABCvsBL” in Table 8.
In an aspect the disclosure provides a method of determining whether a B cell malignancy in a sample is activated B cell-like diffuse large B cell lymphoma (DLBCL), or germinal center-like DLBCL, comprising determining the level of expression of at least one microRNA in the sample relative to the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in Table 35.
In an aspect the disclosure provides a microarray comprising miRNA-specific probe oligonucleotides wherein at least one miRNA-specific probe oligonucleotide is specific for a sequence of SEQ ID NOs: 763-1350 or 1565, or any combination thereof.
In an aspect the disclosure provides a kit comprising at least one primer sequence that can detect any one of SEQ ID NOs: 763-1350 or 1565, or a combination thereof.
In an aspect the disclosure provides a kit comprising at least one isolated nucleic acid molecule having a sequence of any one of SEQ ID NOs: 1351-1564.
In an aspect, the disclosure provides a method of identifying a B cell malignancy comprising determining the level of expression of at least one microRNA selected from the microRNAs listed in Table 4. In certain embodiments, a method comprises determining the level of expression of at least one microRNA selected from the microRNAs listed in Tables 7-35.
Other aspects and embodiments will be apparent to one of skill in the art in light of the following detailed description.
DESCRIPTION OF THE FIGURES FIG. 1 shows that mature B cell subsets demonstrate distinct microRNA profiles. FIG. 1A is a diagram showing the overall schema of mature B cell differentiation. FIG. 1B shows selection of B cell subsets using flow cytometry. FIG. 1C shows the distinction between naive and memory B cells based on IgD and CD27 expression using flow cytometry. FIG. 1D shows relative expression of microRNA in the naive to germinal center B cell transition. FIG. 1E shows relative expression of mRNA in the naive to germinal center B cell transition. FIG. 1F shows relative expression of microRNA in the germinal center B cell to plasma cell transition. FIG. 1G shows relative expression of mRNA in the germinal center B cell to plasma cell transition. FIG. 1H shows relative expression of microRNA in the germinal center B cell to memory B cell transition. FIG. 1I shows relative expression of mRNA in the germinal center B cell to memory B cell transition. In FIGS. 1D, 1F, and 1H, miRNAs that were, on average, at least 2-fold differentially expressed at a false discovery rate of less than 5% are shown according to the color scale. In Figures and 1E, 1G, and 1I, mRNAs that were, on average, at least 2-fold differentially expressed at a false discovery rate of less than 1% are shown according to the color scale. FIG. 1J shows that expression of certain microRNA processing genes, DGCR8, DICER1, EIF2C2, DROSHA, and XP05 is unchanged among the B cell subsets (P>0.1 in all cases).
FIG. 2 shows experimental validation of the interaction of miR-223, which is expressed highly in naive and memory B cells compared to germinal center B cells, and targets the transcription factor LM02. FIG. 2A shows base-pairing of the 3′UTR of the LM02 gene with nucleotides 1-8 of miR-223. This 8-mer is highly conserved across a number of species and serves as a potential binding site for miR-223. FIG. 2B shows the effects of over-expression of miR-223 in germinal center lymphoma-derived BJAB cells in 3 separate experiments. FIG. 2C shows the relative LM02 protein expression from a representative experiment (from 3 replicates) transfecting a scrambled control versus a precursor for miR-223 in BJAB cells. FIG. 2D shows average expression of LM02 relative to Actin over three Western blots of BJAB cells transfected with a scrambled control versus a precursor for miR-223. FIG. 2E shows luciferase activity in BJAB cells transfected with a vector comprising either a luciferase gene coupled to the 3 ′UTR of the LM02 gene or a luciferase gene coupled to the 3 ′UTR of the LM02 gene with the miR-223 binding site mutated, and cotransfected with miR-223.
FIG. 3 shows experimental validation of the interaction of miR-9 and miR-30, which are expressed highly in germinal center B cells compared to plasma cells and target the transcription factor PRDM1. FIG. 3A shows base-pairing of the 3′UTR of the PRDM1 gene with the 5′ seed region of miR-9 and the miR-30 family. FIG. 3B shows the effects of over-expressing miR-9 and 2 members of the miR-30 family, miR-30b and miR-30d, in plasma cell myeloma-derived U266 cells in 3 separate experiments. FIG. 3C shows the relative PRDM1 protein expression from a representative experiment (from 3 replicates) transfecting a scrambled control versus a precursor for miR-9, miR-30b, or miR-30d in U266 cells. FIG. 3D shows the average expression of PRDM1 relative to Actin over three Western blots of U266 cells transfected with a scrambled control versus a precursor for miR-9, miR-30b, or miR-30d. (P<0.05 for miR-30b and miR-30d, P=0.08 for miR-9.) FIG. 3E shows repression of luciferase activity from the PRDM1 3′UTR construct by overexpression of miR-9, miR-30b, and miR-30d wild-type and mutant sequences.
FIG. 4 shows that expression of microRNAs in normal B cells is conserved in certain B cell malignancies. FIG. 4A shows lineage prediction of both IgV mutated and unmutated chronic lymphocytic leukemia, germinal center B cell derived DLBCL, and Burkitt lymphoma based on differential expression of microRNAs in normal naïve B cells and germinal center B cells (microRNAs depicted in FIG. 1D). FIG. 4B shows miRNAs that were found to be differentially expressed (P<0.05) in malignant cells and normal cells as discussed in Example 5. FIG. 4C shows cloning frequency of miRNAs in unselected mature B cells (N=3) and certain B cell malignancies (N=42) from a previously published study (“sequencing data”), as discussed in Example 5. FIG. 4D shows differentially expressed miRNAs that distinguish Burkitt lymphoma, activated B cell-like (ABC) diffuse large B cell lymphoma (DLBCL), germinal center-like DLBCL (GCB DLBCL), and chronic lymphocytic leukemia. Predictor miRNAs from each pair-wise comparison that distinguish each entity are shown in the boxes.
FIG. 5 shows the distribution of miRNAs present in B-cell subsets.
FIG. 6 shows that certain microRNA targets are expressed at lower levels. FIG. 6A shows density plots of the expression frequency of predicted mRNA targets of miRNAs expressed highly in germinal center B cells compared to naive cells. FIG. 6B shows density plots of the expression frequency of predicted mRNA targets of miRNAs expressed highly in germinal center B cells compared to plasma cells. FIG. 6C shows density plots of the expression frequency of predicted mRNA targets of miRNAs expressed highly in the germinal center B cells compared to memory B cells.
FIG. 7 shows that differentially expressed transcription factors are frequently microRNA targets. In FIG. 7A, the left panel indicates the proportion of transcription factors that are differentially expressed in the naive to germinal center B-cell transition that are also predicted targets of differentially expressed miRNAs. The right panel indicates the proportion of transcription factors that are not differentially expressed and also are predicted targets of differentially expressed miRNA in that stage-transition. The p-value indicates the results of a chi-squared test for the enrichment of predicted miRNA targets among the differentially expressed transcription factors. FIG. 7B shows a similar analysis of the germinal center to plasma cell transition. FIG. 7C shows a similar analysis of the germinal center to memory cell transition.
FIG. 8 shows that Mybl transcript levels decrease with miR-223 overexpression. FIG. 8A shows base-pairing of the 3′UTR of the MYBL1 gene with nucleotides 2-8 of miR-223. This 7-mer is highly conserved across a number of species and serves as a potential binding site for miR-223. FIG. 8B shows the effects of over-expression of miR-223 in germinal center lymphoma-derived BJAB cells in 3 separate experiments.
FIG. 9 shows the specificity of real-time PCR probes for members of the miR-30 family.
FIG. 10 shows the results of leave one out cross validation applied to the predictors for Burkitt lymphoma, chronic lymphocytic leukemia, activated B-cell diffuse large B-cell lymphoma, and germinal center B-cell DLBCL.
FIG. 11 shows differentially expressed miRNAs that distinguish activated B cell-like diffuse large B cell lymphoma (DLBCL), germinal center-like DLBCL (GCB DLBCL), Burkitt lymphoma, chronic lymphocytic leukemia, follicular lymphoma, and Hodgkin's lymphoma. Predictor miRNAs from each pair-wise comparison that distinguish each entity are shown in the boxes.
DETAILED DESCRIPTION All patent and non-patent literature references that are cited herein are incorporated herein by reference in their entirety.
In a general sense, the disclosure relates to nucleic acid sequences, such as microRNAs (miRNA), as well as to the identification and analysis of microRNA expression levels and/or patterns in B cells. Through concomitant microRNA and mRNA profiling, the inventors have identified regulatory roles for microRNAs at each stage in mature B cell differentiation. This provides methods identifying microRNA-mediated regulation of oncogenes and key transcription factors in B cell differentiation. This work establishes the landscape of normal microRNA expression in mature B cells and its role in regulating normal B cell differentiation. Further, our work demonstrates that in contrast to the described down-regulation in other malignancies, stage-specific microRNAs are retained in B cell malignancies. The lineage of common B cell malignancies can be predicted based upon miRNA profiles of normal B cells, pointing to a role for microRNAs in the maintenance of mature B cell phenotypes in normal and malignant B cells.
In an aspect, the disclosure relates to an isolated nucleic acid molecule comprising: (a) a nucleotide sequence as shown in Table 32; (b) a nucleotide sequence which is the complement of (a), (c) a nucleotide sequence comprising a sequence identity of at least 80%, (e.g., 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, or at least 99%), to a sequence of (a) or (b) and/or (d) a nucleotide sequence which hybridizes under stringent conditions to a sequence of (a), (b) and/or (c). In some embodiments, the identity of sequence (c) to a sequence of (a) or (b) is at least 90%. In other embodiments, the identity of sequence (c) to a sequence of (a) or (b) at least 95%. The percent identity can be calculated by any routine method used by one of skill in the art such as, for example, the methods described herein.
In embodiments, the isolated nucleic acid molecule relates to a miRNA molecule and analogs thereof, a miRNA precursor molecule, or a primary miRNA molecule, as well as to DNA molecules encoding miRNA, miRNA precursor, or primary miRNA molecules. Accordingly, in such embodiments, the isolated nucleic acid molecule can function as a miRNA molecule under suitable conditions. Suitable conditions include, but are not limited to, various buffer systems that approximate physiologically relevant ionic concentrations and pHs, as well as physiological conditions.
In some embodiments, the nucleic acid molecule comprises a sequence that hybridizes to a nucleotide sequence as shown in Table 32, a complementary sequence thereof or a nucleic acid molecule having at least 80% sequence identity under stringent hybridization conditions. The basic parameters affecting the choice of hybridization conditions and guidance for devising suitable conditions are set forth by Sambrook, et al. (See, 1989, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.; and Current Protocols in Molecular Biology, 1995, Ausubel et al., eds., John Wiley & Sons, Inc.), and can be readily determined by those of ordinary skill in the art based on, for example, the length and/or base composition of the DNA. Generally, stringent salt concentration will ordinarily be less than about 750 mM NaCl and 75 mM trisodium citrate, or less than about 500 mM NaCl and 50 mM trisodium citrate, or even less than about 250 mM NaCl and 25 mM trisodium citrate. High stringency hybridization conditions can be obtained by adding an amount of organic solvent (e.g., at least about 35% to about 50% formamide). Stringent temperature conditions will ordinarily include temperatures of at least about 30° C., (e.g., at least about 37° C., 42° C., 45° C., 50° C., or 55° C.). Varying additional parameters, such as hybridization time, the concentration of detergent (e.g., 0.1-1.0% sodium dodecyl sulfate (SDS)), and the inclusion or exclusion of carrier DNA (e.g., about 100-200 μg/ml denatured salmon sperm DNA (ssDNA)), are well known to those skilled in the art. Stringent hybridization conditions are known in the art and include non-limiting examples such as, washing for 1 hr in 300 mM NaCl, 30 mM trisodium citrate and 0.1% SDS at 45-50° C.; washing for 1 h in 300 mM NaCl, 30 mM trisodium citrate and 0.1% SDS at 45-50° C.; in 500 mM NaCl, 50 mM trisodium citrate, 1% SDS, 35% formamide, and 100 μg/ml denatured salmon sperm DNA (ssDNA) at 37° C.; or in 250 mM NaCl, 25 mM trisodium citrate, 1% SDS, 50% formamide, and 200 μg/ml ssDNA at 42° C. Useful variations on these conditions will be readily apparent to those skilled in the art.
The isolated nucleic acid molecules provided herein suitably have a length of from about 18 to about 100 nucleotides. In embodiments wherein the isolated nucleic acid molecules are miRNAs, the lengths of the miRNAs are suitably in an expected range for the particular type of miRNA molecule. For example, mature miRNAs are typically from about 15 to about 28 nucleotides in length, and suitably have a length of about 19 to about 24 nucleotides (e.g., 19, 20, 21, 22, 23, or 24 nucleotides). Precursor miRNAs typically comprise the mature miRNA sequence and contain a stem-loop structure, suitably of length of about 50 to about 90 nucleotides (e.g., 50, 55, 60, 65, 70, 75, 80, 85, or 90 nucleotides). Primary miRNAs (e.g., a primary transcript comprising a precursor miRNA) can suitably have a length of greater than 100 nucleotides.
The nucleic acid molecules can be provided in either a single-stranded or double-stranded form. Typically, a miRNA as such is identified as a single-stranded molecule, while the precursor miRNA is typically at least partially self-complementary and capable of forming double-stranded portions, e.g. stem- and loop-structures. DNA molecules encoding the miRNA and miRNA precursor molecules (e.g., expression vectors, cloning vectors, and the like) are typically double-stranded. The individual nucleic acids that comprise the isolated nucleic acid molecules can be selected from RNA, DNA, or nucleic acid analog molecules, such as chemically modified sugar (e.g., 2′-modified (2′-F, 2′-OMe, etc.) or backbone (e.g., phosphorothioates), or cap (e.g., 5′- and/or 3′-abasic groups) moieties of ribonucleotides or deoxyribonucleotides. Other nucleic acid analogs, such as peptide nucleic acids (PNA) or locked nucleic acids (LNA), are also suitable in various embodiments described herein. In some embodiments the nucleic acid molecules can comprise any combination of nucleic acid analog(s).
While many of the nucleic acid molecules in the Tables are identified as RNA sequences, e.g., miRNAs, the disclosure of those sequences should be understood to encompass the corresponding DNA (e.g., cDNA) sequences, wherein the uracil (U) nucleotides of the disclosed RNAs are substituted by thymidine (T) nucleotides in the corresponding DNA. One of skill in the art is able to generate such DNA sequences (e.g., cDNA) through routine microbiological techniques known in the art such as, for example, reverse transcription using methods that incorporate the reverse transcriptase enzyme.
In another aspect, the disclosure provides a recombinant expression vector comprising a recombinant nucleic acid sequence operatively linked to an expression control sequence, wherein expression of the recombinant nucleic acid sequence provides a miRNA sequence, a precursor miRNA sequence, or a primary miRNA sequence as described herein. The resulting sequence (e.g., primary or precursor miRNAs) can optionally be further processed to provide the miRNA sequence. In embodiments, the recombinant expression vector comprises at least one sequence in Table 32. Any suitable expression vector can be used such as, for example, a DNA vector (e.g., viral vector, plasmid, etc.). In some embodiments the expression vector is selected for expression in a eukaryotic cell such as, for example, a mammalian cell. One of skill in the art will be able to select an appropriate vector based on the particular application and/or expression system to be employed.
Thus, embodiments provide nucleic acid constructs in the form of plasmids, vectors, transcription or expression cassettes which comprise at least one nucleotide sequence encoding a miRNA described herein, or fragments thereof, and a suitable promoter region. Suitable vectors can be chosen or constructed, which contain appropriate regulatory sequences, such as promoter sequences, terminator sequences, polyadenylation sequences, enhancer sequences, marker genes and other sequences as desired. Vectors can be plasmids, phage (e.g. phage, or phagemid) or viral (e.g. lentivirus, adenovirus, AAV) or any other appropriate vector. For further details see, for example, Molecular Cloning: a Laboratory Manual: 2nd edition, Sambrook et al., 1989, Cold Spring Harbor Laboratory Press.
Relatedness of Nucleic Acid Molecules/Sequences
The term “identity” refers to a relationship between the sequences of two or more two or more nucleic acid molecules, as determined by comparing the sequences. In the art, “identity” also means the degree of sequence relatedness between amino acid or nucleic acid molecule sequences, as the case may be, as determined by the match between strings of nucleotide or amino acid sequences. “Identity” measures the percent of identical matches between two or more sequences with gap alignments addressed by a particular mathematical model or computer programs (i.e., “algorithms”).
Identity of related nucleic acid molecules can be readily calculated by known methods, including but not limited to those described in Computational Molecular Biology, Lesk, A. M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects, Smith, D. W., ed., Academic Press, New York, 19933; Computer Analysis of Sequence Data, Part 1, Griffin, A. M., and Griffin, H. G., eds., Humana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M. Stockton Press, New York, 1991; and Carillo, H., and Lipman, D., SIAM J. Applied Math., 48:1073 (1988).
Non-limiting methods for determining identity are designed to give the largest match between the sequences tested. Methods to determine identity are codified in publicly available computer programs. Preferred computer program methods to determine identity between two sequences include, but are not limited to, the GCG program package, including GAP (Devereux, et al., Nucleic Acids Research 12:387 [1984]; Genetics Computer Group, University of Wisconsin, Madison, Wis.), BLASTN, and FASTA (Atschul et al., J. Molec. Biol. 215:403-410 [1990]). The BLAST X program is publicly available from the National Center for Biotechnology Information (NCBI) and other sources (BLAST Manual, Altschul] et al., NCB NLM NIH Bethesda, Md. 20894; Altschul et al., J. Mol. Biol. 215:403-410 [1990]). The well known Smith Waterman algorithm may also be used to determine identity.
Exemplary parameters for nucleic acid molecule sequence comparison include the following:
Algorithm: Needleman and Wunsch, J. Mol Biol. 48:443-453 (1970)
Comparison matrix: matches=+10, mismatch=0
Gap Penalty: 50
Gap Length Penalty: 3
The GAP program is also useful with the above parameters. The aforementioned parameters are the default parameters for nucleic acid molecule comparisons.
Other exemplary algorithms, gap opening penalties, gap extension penalties, comparison matrices, thresholds of similarity, etc. can be used by those of skill in the art, including those set forth in the Program Manual, Wisconsin Package, Version 9, September 1997. The particular choices to be made will depend on the specific comparison to be made, such as DNA to DNA or RNA to DNA; and additionally, whether the comparison is between given pairs of sequences (in which case GAP or BestFit are generally preferred) or between one sequence and a large database of sequences (in which case FASTA or BLASTA are preferred).
In an aspect, the disclosure provides a vector comprising the isolated polynucleotide as described herein such as, for example one or more of SEQ ID NOs 773-1046 or 1450-1542. In embodiments, the vector can be any type of vector that finds use as a vehicle to transfer foreign genetic material into a cell. Non-limiting examples of vectors include plasmids, viral vectors (e.g., derived from lentivirus, adenovirus, adeno-associated virus (AAV), retrovirus, etc.), bacteriophage, cosmids, and artificial chromosomes. In embodiments, the vector can be an expression (or expression constructs) for driving expression of the polynucleotide in a target cell. Vectors and methods for inserting them into a target cell are known in the art [See, e.g., Sambrook et al., 1989].
In an aspect, the disclosure provides recombinant cells that comprise the vectors and/or polynucleotides described herein. The cells can be any cell suitable as a host for recombinant nucleic acid molecules, and selected based on well known techniques. Techniques for generating and maintaining recombinant cells are known in the art, such as those described in Sambrook et al., 1989.
The term “B cell malignancy,” as used herein, refers to a malignancy derived from any stage of B cell, including, but not limited to, naïve cells, germinal center cells, memory B cells, and plasma cells. Examples of B cell malignancies include, but are not limited to, mantle cell lymphoma, follicular lymphoma, Hodgkin's lymphoma, Burkitt lymphoma, germinal center B-cell like diffuse large B cell lymphoma (DLBCL), chronic lymphocytic leukemia, small lymphocytic lymphoma, lymphoplasmacytic lymphoma, multiple myeloma, and activated B-cell like DLBCL.
In an aspect, the disclosure provides a method of distinguishing B cell malignancies on the basis of the B-cell origin. In certain embodiments, methods of diagnosing B cell malignancies on the basis of the B-cell origin are provided. In certain such embodiments, a B cell malignancy is determined to be derived from a particular B-cell stage. The B-cell origin of a B cell malignancy may be determined, in certain embodiments, by detecting one or more microRNAs that can be used to distinguish B-cell stages. Certain exemplary B-cell stages include, but are not limited to, naïve cells, germinal center cells, memory B cells, and plasma cells. Certain exemplary microRNAs that can be used to distinguish B-cell stages are shown in Table 4. In various embodiments, the method comprises detecting at least one, at least two, at least five, at least 10, at least 20, at least 30, at least 50, at least 75, or at least 100 microRNAs.
In certain embodiments, a panel of microRNAs is selected that will allow determination of the B cell stage from which a B cell malignancy is derived. For example, in certain embodiments, two or more microRNAs from Table 4 are selected such that detection of the levels of those microRNAs in a B cell malignancy will indicate whether the B cell malignancy is derived from naïve, germinal center, plasma, or memory B cells. In various embodiments, the panel of microRNAs comprises at least one, at least two, at least five, at least 10, at least 20, at least 30, at least 50, at least 75, or at least 100 microRNAs from Table 4. One skilled in the art can select a suitable panel of microRNAs, including one or more microRNAs from Table 4, according to the intended use of the panel.
As described throughout the disclosure, the methods herein can include detecting one or a plurality of miRNAs. When the term “at least” is used in association with a number (e.g., “at least 20”) that term will be understood to include 20 as well as optionally any integer after 20 and up to and including the total number of microRNAs disclosed herein.
In some embodiments, a B cell malignancy derived from naïve cells is mantle cell lymphoma. In other embodiments, a B cell malignancy derived from germinal center cells includes, but is not limited to, follicular lymphoma, Hodgkin's lymphoma, Burkitt lymphoma, or germinal center B-cell like diffuse large B cell lymphoma (DLBCL). In other embodiments, a B cell malignancy derived from memory B cells includes, but is not limited to, chronic lymphocytic leukemia or small lymphocytic lymphoma. In some embodiments, a B cell malignancy derived from plasma cells includes, but is not limited to, multiple myeloma or activated B-cell DLBCL.
Certain B cell malignancies can be difficult to distinguish using current methodologies. In extreme cases, almost any B cell malignancy can be confused with another. As illustrative examples, Burkitt lymphoma and DLBCLs are often confused. Similarly, mantle cell lymphoma and small lymphocytic lymphoma can also be confused. Burkitt lymphoma and germinal center DLBCL are both derived from germinal center cells, while activated B-cell DLBCL is derived from plasma cells. Thus, if a B cell malignancy appears to be Burkitt lymphoma or a DLBCL, in certain embodiments, microRNA analysis can be used to narrow down the B cell malignancy to either a germinal center cell-derived B cell malignancy or a plasma cell-derived B cell malignancy. If the B cell malignancy is plasma-cell derived, then it may be activated B-cell DLBCL.
Thus, in certain embodiments, when the identity of a particular B cell malignancy has been narrowed down to two or more possible B cell malignancies, and at least two of those B cell malignancies are derived from different B cell stages, microRNAs that distinguish certain B cell stages can be used to further narrow down the identity of the B cell malignancy. In certain embodiments, microRNAs that distinguish certain B cell stages can be used to identify the B cell malignancy. One or more such microRNAs can be selected, in certain embodiments, from the microRNAs in Table 4. One skilled in the art can select a suitable set of microRNAs, including at least one microRNA from Table 4, for distinguishing particular B cell stages.
In an aspect, the disclosure provides a method of identifying a B cell malignancy comprising detecting one or a plurality of microRNAs. In certain embodiments, the method can provide a diagnosis of a B cell malignancy. In certain embodiments, one or more microRNAs that are characteristic of a particular B cell malignancy are used to identify the B cell malignancy. In certain embodiments, the identity of the B cell malignancy is first narrowed down to a list of two or more particular B cell malignancies using, for example, tumor morphology and/or immunohistochemistry and/or microRNA detection, e.g., to determine the B cell stage from which the tumor is derived. Certain exemplary microRNAs that can be used to identify B cell malignancies are shown in the Tables (e.g., Tables 7-35).
In certain embodiments, methods of identifying B cell malignancies comprise detecting one or more microRNAs from one or more of Tables 7 to 15, and Appendix B, Tables 16 to 30. That is, in certain embodiments, a panel of microRNAs is selected that will identify a B cell malignancy as being one of a particular selection of B cell malignancies. As a non-limiting example, a panel of microRNAs can be designed to identify a B cell malignancy as one of Burkitt lymphoma, ABC DLBCL, or GCB DLBCL. In certain such embodiments, the panel of microRNAs comprises at least one microRNA from Table 10, column “BL miRNA list” and/or Table 14, column “BL High”; at least one microRNA from Table 11 and/or Table 14, column “ABC High”; and at least one microRNA from Table 10, column “GCB miRNA list” and/or Table 14, column “GCB High”.
When other methods indicate a particular identity for a B cell malignancy, in certain embodiments, microRNAs can be used to confirm that identification. Thus, for example, if a B cell malignancy is believed to be a Burkitt lymphoma, that identification can be confirmed by determining the expression level of one or more microRNAs listed in Table 10, column “BL miRNA list” and/or Table 14, column “BL High”. Similarly, if a B cell malignancy is believed to be ABC DLBCL, that identification can be confirmed by determining the expression level of one or more microRNAs listed in Table 11 and/or Table 14, column “ABC High”. If a B cell malignancy is believed to be GCB DLBCL, that identification can be confirmed by determining the expression level of one or more microRNAs listed in Table 10, column “GCB miRNA list” and/or Table 14, column “GCB High”. If a B cell malignancy is believed to be chronic lymphocytic leukemia, that identification can be confirmed by determining the expression level of one or more microRNAs listed in Table 12 and/or Table 15, column “CLL High”. If a B cell malignancy is believed to be Hodgkin's lymphoma, that identification can be confirmed by determining the expression level of one or more microRNAs listed in Table 13 and/or Table 15, column “HL High”. If a B cell malignancy is believed to be follicular lymphoma, that identification can be confirmed by determining the expression level of one or more microRNAs listed in Table 10, column “FL miRNA list” and/or Table 15, column “FL High”.
In certain embodiments, when the identity of a B cell malignancy has been narrowed down to two B cell malignancies selected from Burkitt lymphoma, GCB DLBCL, ABC DLBCL, chronic lymphocytic leukemia, follicular lymphoma, and Hodgkin's lymphoma, the identity of the B cell malignancy can be determined by detecting one or more microRNAs from Tables 16 to 30.
As discussed in the Examples, the miRNAs described herein as differentially expressed in a B cell malignancy have been identified with high confidence, and thus, identification of one miRNA is adequate to perform the methods of identification and diagnosis disclosed herein. Accordingly, in various embodiments, the methods can comprise detecting at least one, at least two, at least five, at least 10, at least 20, at least 30, or at least 50 microRNAs in order to narrow down the identity of, or identify, a B cell malignancy.
As noted above, the treatment regimens and prognoses for the various B cell malignancies can differ significantly. Thus, determining the correct identity and/or origin of a B cell malignancy can be important for selecting an effective therapy and/or setting appropriate patient expectations.
B cell malignancy samples may be obtained and prepared using methods known in the art. One skilled in the art can select an appropriate method of obtaining a B cell malignancy sample according to various parameters, such as the age, size, medical history, and/or identity of the patient. One skilled in the art can select an appropriate method of preparing a B cell malignancy sample for analysis according to the B cell malignancy sample source, size, quality, and/or intended use. For example, in certain embodiments, a B cell malignancy sample is prepared in a manner that preserves microRNAs in the sample as much as practicable under the circumstances.
MicroRNAs can be detected using any method known in the art. Exemplary methods of detecting microRNAs include, but are not limited to, hybridization-based methods and amplification-based methods. Certain exemplary detection methods include, but are not limited to, arrays (including microarrays and bead-based arrays), in situ hybridization, Northern blotting, TaqMan probes, RT-PCR, real-time PCR, and direct sequencing. One skilled in the art can select a suitable detection method according to the sample source, size, quality, and/or particular application.
In certain embodiments, real-time PCR is employed to determine the expression level of a microRNA. In some embodiments a miRNA is considered present in a subpopulation if the cycling time (CT) is less than 36 in all three biological replicates, and a CT greater than 36 is undetected.
In certain embodiments, the expression level of a microRNA in a sample is determined relative to a control sample. A control sample may be selected, in various embodiments, because it is expected to have either high or low expression of the microRNA.
In certain embodiments, the expression level of a microRNA may be normalized to the expression level of a polynucleotide that is expected to be expressed at similar levels in several different cell types and/or at constant levels in the cell type being analyzed.
In certain embodiments, an identified miRNA from Tables 7-35 is used to distinguish one of the six exemplified B cell malignancies from the other malignancies. A “high” and a “low” in Tables 10 to 13 refer to at least a 2-fold difference in the expression of the identified miRNA when one lymphoma is compared to other lymphomas and benign lymph nodes.
In embodiments, mRNA levels can be profiled by using a microarray. In some embodiments, array elements with median signal intensities of less than 7 log 2 units across samples are removed from analysis. In embodiments, a gene is considered for further analysis if it is on-average 2-fold or higher differentially expressed in a binary comparison of B cell subsets and expressed in at least one of the two B cell subsets being compared.
In an embodiment, the level of at least one miRNA is measured by reverse transcribing RNA from a test sample obtained from a subject to provide a set of target oligodeoxynucleotides, hybridizing the target oligodeoxynucleotides to one or more miRNA-specific probe oligonucleotides (e.g., a microarray that comprises miRNA-specific probe oligonucleotides) to provide a hybridization profile for the test sample, and comparing the test sample hybridization profile to a hybridization profile generated from a control sample. An alteration in the signal of at least one miRNA in the test sample relative to the control sample is indicative of the subject either having, or being at risk for developing, a B-cell malignancy. In an embodiment, the signal of at least one miRNA is upregulated, relative to the signal generated from the control sample. In another embodiment, the signal of at least one miRNA is down-regulated, relative to the signal generated from the control sample. In some embodiments, the microarray comprises miRNA-specific probe oligonucleotides for a substantial portion of all known human miRNAs. In a further embodiment, the microarray comprises miRNA-specific probe oligonucleotides for one or more miRNAs selected from the group consisting of SEQ ID NOs: 763-1350, or 1565 and any combination thereof.
The microarray can be prepared from gene-specific oligonucleotide probes generated from known miRNA sequences. The array may contain two different oligonucleotide probes for each miRNA, one containing the active, mature sequence and the other being specific for the precursor of the miRNA. The array may also contain controls, such as one or more mouse sequences differing from human orthologs by only a few bases, which can serve as controls for hybridization stringency conditions. tRNAs and other RNAs (e.g., rRNAs, mRNAs) from both species may also be printed on the microchip, providing an internal, relatively stable, positive control for specific hybridization. One or more appropriate controls for non-specific hybridization may also be included on the microchip. For this purpose, sequences are selected based upon the absence of any homology with any known miRNAs.
The microarray may be fabricated using techniques known in the art. For example, probe oligonucleotides of an appropriate length, e.g., 40 nucleotides, are 5′-amine modified at position C6 and printed using commercially available microarray systems. Labeled cDNA corresponding to the target RNA sequence(s) is prepared by reverse transcribing the target RNA with labeled primer. Following first strand synthesis, the RNA/DNA hybrids are denatured to degrade the RNA templates. The labeled target cDNAs thus prepared are then hybridized to the microarray chip under typical hybridizing conditions. At positions on the array where the immobilized probe DNA recognizes a complementary target cDNA in the sample, hybridization occurs. The labeled target cDNA marks the exact position on the array where binding occurs, allowing automatic detection and quantification. The output consists of a list of hybridization events, indicating the relative abundance of specific cDNA sequences, and therefore the relative abundance of the corresponding complementary miRNAs, in the patient sample.
In an aspect, the disclosure relates to kits. Such kits can be used in methods of identifying a miRNA or mRNA described herein; an expression level or expression pattern of one or more miRNA(s) or mRNA(s) described herein; and/or identifying a B-cell malignancy. In some embodiments the kit can provide a diagnosis of a B-cell type and or a B-cell malignancy. In some embodiments the kit can differentiate one B-cell malignancy from other B-cell malignancies (e.g., ABC-DLBCL from GBC-DLBCL), and can provide information useful to a medical professional regarding a preferred course of therapeutic treatment. Suitably, a kit can comprise an isolated nucleic acid molecule or a plurality of isolated nucleic acid molecules as described herein (e.g., a sequence complementary to any of the miRNAs disclosed in the Tables). In embodiments, the isolated nucleic acid molecule can comprise a sequence of one or more RT-PCR target sequences, primers directed thereto, or a sequence complementary thereto. The kit can also include adapter nucleic acid molecules (e.g., universal adapter molecules for attachment to expressed miRNAs/mRNAs for reverse transcription and amplification); appropriate buffer systems and reagents, detectable labels, an energy source (e.g., ATP), and other agents and components that can be used in performing analysis of miRNA expression (e.g., in RT-PCR, deep sequencing, or microarray-based methods). Kits also include instructions for use.
It will be understood that any numerical value recited herein includes all values from the lower value to the upper value. For example, if a concentration range is stated as 1% to 50%, it is intended that values such as 2% to 40%, 10% to 30%, or 1% to 3%, etc., are expressly enumerated in this specification. These are only examples of what is specifically intended, and all possible combinations of numerical values between the lowest value and the highest value enumerated are to be considered to be expressly stated in this application.
In an aspect, the disclosure provides a DNA library comprising one or more miRNA sequences from Tables 4-5, or 6-33. In a related aspect, the disclosure provides a method for generating such a DNA library. In an embodiment the library comprises a cDNA library that includes sequences derived from a sample of the miRNAs or, in addition or alternatively, the mRNA purified from a particular source such as, for example, a collection of cells, a particular tissue, or an entire organism. In embodiments, the source of the cDNA library is a B cell, such as a B cell in any stage (e.g., naïve, germinal center, memory, activated, or plasma, etc.) or a B cell malignancy (e.g., mantle cell lymphoma, follicular lymphoma, Hodgkin's lymphoma, Burkitt lymphoma, germinal center B-cell like diffuse large B cell lymphoma (DLBCL), chronic lymphocytic leukemia, small lymphocytic lymphoma, lymphoplasmacytic lymphoma, multiple myeloma, and activated B-cell like DLBCL). Typically, the isolated miRNA (or mRNA) is converted to a DNA template by reverse transcription, and comprises the cDNA version of the expressed RNA (e.g., miRNA or mRNA). Thus, a library can represent the cDNA version of the active “transcriptome” in a particular source under the physiological, developmental, or environmental conditions that existed when the miRNA/mRNA was purified.
In an embodiment, the library comprises a miRNA sequence described in Tables 4-5; 7-33. In embodiments, the library comprises at least one of SEQ ID NOs 763-1350 or 1565, and any combination thereof. In an embodiment, the library comprises a collection of miRNA sequences comprising SEQ ID NOs 763-1350 or 1565. In embodiments the library can be used to identify and/or differentiate a B-cell malignancy from other B-cell malignancies. In such embodiments, the library comprises at least one miRNA sequence selected from those listed in any of Tables 4 or 7-35.
As used herein, a “library” is a collection of DNA sequences that is stored and propagated in a population of microorganisms through standard molecular cloning processes. A DNA library can be of any type such as, for example, a cDNA library (formed from reverse-transcribed RNA) or a genomic library (formed from genomic DNA). The DNA library can be used in any routine application or technique known in the art (e.g., gene discovery; cloning of full-length cDNAs to identify/study gene function; miRNA/mRNA expression in different cells or tissues; splice variants in different cells or tissues) and, in some embodiments, can depend on the source of the original DNA fragments. In embodiments, the library can be used to isolate, characterize, and/or quantify the actively expressed miRNA is a population of cells such as, for example, B-cells or B-cell malignancies. In some embodiments, the library can be used to study miRNA-protein interactions or miRNA-based regulation of protein expression or activity.
Any known method of library preparation can be used to make the library described herein, including the methods described in the detailed description and non-limiting Examples. Further general techniques can be based on the methods and techniques known in the art, (see, e.g., RNA Methodologies: A Laboratory Guide for Isolation and Characterization (R. E. Farrell, Academic Press, 1998); cDNA Library Protocols (Cowell & Austin, eds., Humana Press; Functional Genomics (Hunt & Livesey, eds., 2000); and the Annual Review of Genomics and Human Genetics (E. Lander, ed., yearly publications by Annual Reviews). Suitably, the nucleotide sequences of interest in a library are preserved as inserts in a plasmid or the genome of a bacteriophage that has been used to infect bacterial cells. There are differences in the cloning vectors and techniques used in library preparation, but in general each DNA fragment is uniquely inserted into a cloning vector and the pool of recombinant DNA molecules is then transferred into a population of bacteria or yeast such that each organism contains on average one construct (vector+insert). The DNA molecules are copied and propagated along with the population of organisms in culture (thus, effectively, “cloned”). Accordingly, in some embodiments, the term “library” can refer to a population of organisms, each of which carries a DNA molecule inserted into a cloning vector, or alternatively to the collection of all of the cloned vector molecules.
An “increased level” of expression, as used herein, refers to a level of expression that is at least 2-fold greater than the level of expression in a control cell type or tissue. In various embodiments, the level of expression is at least 2.5-fold, at least 3-fold, at least 5-fold, or at least 10-fold, greater than the level of expression in a control cell. Exemplary control cells and tissues include, but are not limited to, normal cells, benign lymph nodes, and other B cell malignancies. In certain embodiments, benign lymph nodes are used as a control tissue. Such benign lymph node tissue contains a variety of cell types.
A “decreased level” of expression, as used herein, refers to a level of expression that is less than 50% of the level of expression in a control cell.
The term “differentially expressed” or “differential expression” relates to a difference in the observed or detected level of expression of a biomolecule such as, for example, nucleic acids (e.g., a polynucleotide, mRNA, miRNA, etc.) or amino acid sequence (e.g., protein, polypeptide, etc.) in a test sample relative to the observed or detected level of expression of the same biomolecule in a control sample or other reference (e.g., a previously established reference level). The difference in expression can be either an increase or a decrease in the expression of the biomolecule in the test sample relative to the control sample.
The Examples that follow provide further illustration of certain aspects and embodiments described in the foregoing description. These illustrative Examples should not be interpreted as limiting the scope of the appended claims.
EXAMPLES Example 1 Materials and Methods Patient Sample Processing
B cell populations were obtained from young patients undergoing routine tonsillectomy using a protocol approved by the Clinical Center at the National Institutes of Health. Patient tonsils were disaggregated and separated by Ficoll. The mononuclear cell layer was harvested, washed in PBS, and resuspended in ACK lysing buffer to remove small numbers of red blood cells. After a wash and resuspension with 10 ml of PBS with 10% Bovine Serum Albumin, cells were counted and 200 million were stained with fluorochrome-tagged monoclonal antibodies to CDI9, IgD, CD38 and CD27. The specific monoclonal antibodies employed were anti-CDI9-PE-Cy5.5, anti-IgD-FITC, anti-CD27-PE, and anti-CD38-APC, all from BD Biosciences and BD Pharmingen (San Jose Calif.). Cells were sorted using the MoFlo Cell sorter (Dako Cytomation, Colorado Springs, Colo.) into naive B cells (CDI9+IgD+CD2TCD38+), germinal center B cells (CDI9+IgD−CD38++), memory B cells (CDI9+IgD−CD27+CD38dim) and plasma cells (CDI9dimIgD−CD27++CD38+++). Three replicates of each B cell subset were obtained from separate patients. The sample purity was verified by FACS and found to be over 90% in all cases.
Tumor specimens were obtained from patients who were examined under a protocol approved by the Duke University Medical Center Institutional Review Board. The pathologic diagnosis of the samples was verified prior to analysis. Samples from patients with diffuse large B cell lymphoma were further subclassified as described previously. See Hans et al. (2004) Blood 103: 275-282. Chronic lymphocytic leukemia samples were processed and purified as described previously. See Volkheimer et al. (2007) Blood 109: 1559-1567. Total RNA was extracted using the phenol-chloroform method to preserve miRNAs, using Ambion reagents.
microRNA Profiling Using Multiplexed Real-Time PCR
MiRNA expression profiling was conducted using the Applied Biosystems 384-well multiplexed real-time PCR assay using 400 ng of total RNA. Eight reactions, each containing 50 ng of RNA and a multiplex looped primer pool with endogenous small nucleolar (sno)-RNA controls, were used to reverse-transcribe the miRNAs in parallel fashion. Each completed reaction was loaded onto the 384-well plate per manufacturer's instructions, and real-time PCR was run on the ABI 7900HT Prism. For each 384-well plate, we used the automatically determined cycle-threshold (CT) using the SDS 2.2.1 software (Applied Biosystems). Consistent with manufacturer recommendations, we considered CT greater than 36 as undetected. A miRNA was considered to be present in a subpopulation if the CT was less than 36 in all three biological replicates. The probes deemed to be present were normalized to the average expression of a sno-RNA control. The expression values were calculated as 2−ΔCT, then median centered to 500 and log 2-transformed.
Gene Expression (mRNA) Profiling Using Microarrays
Gene expression profiling and normalization were performed using methods identical to those we have described previously. See Dave et al. (2004) N. Engl. J. Med. 351: 2159-2169. Array elements with median signal intensities of less than 7 log 2 units across the samples were removed from analysis, in order to exclude poorly measured genes and genes not appreciably expressed in the samples. Genes that were on-average 2-fold or higher differentially expressed in a binary comparison of B cell subsets, and appreciably expressed in at least one of the 2 B cell subsets being compared, were selected for further analysis as described below. The data have been deposited in the publicly available Gene Expression Omnibus database (GSE12366).
MiRNA Profiling Using Microarray
MiRNA expression profiling from human B cell malignancies was conducted using up to 1 μg of total RNA from sample and reference (normal lymph node), which were labeled with Cy3 or Cy5 fluorescent dyes, using the miRNA/LNA labeling kit (Exiqon, Denmark). The fluorescently labeled samples were combined and hybridized to a miRNA microarray (v.10.0, Exiqon, Denmark), in a nitrogen atmosphere. The micro array slides were scanned with GenePix 4100 Scanner. The quantified signals were normalized using the global Lowess algorithm, using Genespring (Agilent) software. The intensity values for multiple spots were averaged and the normalized values were log 2-transformed. Missing values were replaced with the lowest value for analysis.
MiRNA Target Prediction
Annotated genes on the U133plus 2.0 array were matched to the miRNA target list downloaded from TargetScan (www.targetscan.org). For the purpose of this study, a target gene was defined by the presence of a seed sequence match (nucleotides 2-8) and conservation of the seed sequence and 3 ′UTR in humans, dog, rat, mouse and chicken. Additional conservation was examined in miRNA target genes selected for experimental validation. The distribution of the mRNA expression for these genes was plotted as a density plot using the Splus statistical software (Insightful Corporation). The difference in distribution between the B cell subsets was calculated using a two-sample, 1-sided Kolmogorov-Smirnov test to examine the hypothesis that being a miRNA target conferred repression in the appropriate population (consistent with the known biology of miRNA effects).
The 3′UTRs of LM02, MYBL1 and PRDM1 were aligned using Blastz alignment of Human, Chimp, Mouse, Rat, Dog, Chicken, Frog (Xenopus) and Zebrafish, and were displayed using the UCSC genome browser. The conservation of miR-223 seed sequence and the 3′UTRs of LM02 and MYBL1, as well as that of the miR-30 family and miR-9 on PRDM1, were thus verified.
Western Blot
RIPA Lysis buffer (1× phosphate-buffered saline [PBS], 1 Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 10 mM phenylmethylsulfonyl fluoride, 1 μg/mL aprotinin, and 100 mM sodium orthovanadate) was added to 750,000 cells and incubated on ice for 30 minutes. The mixture was spun down and the supernatant was transferred to a new tube as the whole cell extract. A total of 20 μg of cell lysate was separated on a 4-18% Tris-Bis NuPAGE gel (Invitrogen) and transferred using the iBlot transfer device (Invitrogen) program 3 for 7 minutes (LM02 detection) or program 2 for 6 minutes (PRDM1). The blots were probed using 1:200 mouse-anti-LM02 (Santa Cruz Biotechnologies SC-65736), 1:750 mouse-anti-Blimp-1 (Santa Cruz Biotechnologies SC-66015) or 1:5000 goat-anti-B-actin (Santa Cruz Biotechnologies SC-47778) for 1 hour at room temperature. The antibodies were detected using 1:10,000 goat-anti-mouse horseradish peroxidase conjugated antibodies (Santa Cruz Biotechnologies). Western Blotting Luminol Reagent (Santa Cruz Biotechnologies) was used to visualize the bands corresponding to each antibody.
Single miRNA/mRNA Expression Using Real-Time Polymerase Chain Reaction (RT-PCR)
With 10 ng of RNA per reaction, miRNAs of interest were reverse-transcribed with ABI individual stem-loop primers designed to detect only mature miRNA, and measured by Taqman real-time PCR normalized to the small nucleolar RNA, RNU48. In order to assess mRNA expression using RT-PCR, 1 μg of RNA was reverse-transcribed with the ABI High Capacity cDNA Reverse Transcription kit. Gene expression was measured with exon-spanning Taqman probes, and normalized to beta-2 micro globulin expression.
Cell Culture
BJAB and H929 were cultured in RPMI (Gibco) supplemented with 10% fetal bovine serum, and U266 was cultured in RPMI supplemented with 15% fetal bovine serum. 293T cells were grown in DMEM media (Gibco) with 10% FBS. All cell lines were grown in 37° C. humidified cell culture incubators with CO2 maintained at 5%.
MiRNA Functional Analysis
MicroRNA Transfection
miRNAs of interest were over-expressed in cell lines of interest by transfecting the appropriate miRNA precursors (Ambion) at 100 nanomoles using Amaxa's Nucleofector system. In particular, BJAB was transfected with Nucleofector solution T, program T-016, U266 with Nucleofector C, program X-005, and H929 with Nucleofector V, program T-001. 1.5 million cells were used per transfection and mixed with appropriate miRNA precursors (Ambion) for a concentration of 100 nM.
Statistical Analysis
Identifying Differentially Expressed miRNA and mRNA
MiRNAs were considered to be differentially expressed if the mean signal was changed at least 2-fold and a false discovery rate (q) was less than 5% using Significance Analysis of Microarrays (SAM) with 1000 permutations. See Tusher et al. Proc Natl Acad Sci USA. 2001; 98:5116-5121.
Differentially expressed genes (mRNA) in Naive versus Germinal Center, Germinal Center versus Plasma Cells, and Germinal Center versus Memory Cells comparisons were identified using SAM. Genes that were 2-fold differentially expressed at a false discovery rate (q) less than 1% with 1000 permutations were identified as significantly differentially expressed.
Transcription Factors and miRNA Target Genes
Transcription factors were identified based on the gene ontology (GO search term “transcription factor”) and matched to the probes of the Affymetrix U133plus 2.0 microarray. Of the total of 938 transcription factor genes thus identified, we selected 364 genes that were differentially expressed in at least one of the B cell stage transitions. We evaluated the breakdown of the differentially expressed transcription factors among miRNA targets versus non targets. The p-values were computed using a chi-square test separately in each B cell stage-transition.
B Cell Malignancy Sample Classification
The top 50 most differentially expressed miRNAs (P<0.01) in each pair-wise B cell malignancy type comparison were chosen as the initial predictor. Singular value decomposition was applied to reduce the list to 20 most informative miRNAs in each pair-wise comparison. See West et al. Proc Natl Acad Sci USA. 2001; 98:11462-11467. A Bayesian logistic regression was performed in Matlab (Mathworks) using the 20-predictor miRNAs for each pair-wise comparison. Each sample was tested using the microRNA-based predictor in a leave-one-out fashion to determine the accuracy of each prediction. For a sample to be classified as a particular B cell malignancy (or normal) type, it had to be predicted as such in every pair-wise comparison.
Normal B Cell Stage Classification of B Cell Malignancies
We constructed a Bayesian predictor to distinguish normal naive from germinal center B cells based on the 32 miRNAs depicted in FIG. 1D. We then applied the predictor without optimization to the microarray data generated for GCB DLCBL, Burkitt lymphoma and chronic lymphocytic leukemia to render a Bayesian prediction of lineage; i.e. naive versus germinal center B cell.
Western Blot Quantitative Analysis
Western blot scans were quantified using NIH ImageJ software. For each experiment, the ratios of protein of interest (LM02, PRDM1) to Actin were determined and mean centered to 100 across the experiment. The average and standard deviation of these values across the three experiments were calculated and displayed relative to the scrambled control expression.
Luciferase Indicator Assay Quantitative Analysis
Firefly luciferase reporter constructs were created in the pL/SV40/GL3 vector for the LM02 3′UTR and the LM02 3′UTR with the predicted miR-223 binding site mutated, as described below. Mature microRNA expression of a pL/CMV/eGFP vector coding for pri-miR-223 from the 3′UTR of EGFP of the vector was confirmed by Taqman-real time PCR in transfected 293T cells. gl3 activity was normalized in dual luciferase assays to pL/SV40/RLuc, with which it was cotransfected. The PRDM1 3′UTR was also cloned into the pL/SV40/GL3 vector. microRNA expression vectors and their respective seed sequence mutants were created for miR-9-2, miR-30b, and miR-30d.
LM02
The LM02 3′UTR was PCR-amplified from BJAB cDNA using primers 1 and 2 (SEQ ID NOs: 1 and 2, respectively) and ligated into the XhoI and XbaI sites of the previously described lentiviral vector pL/SV40/GL3, which expresses firefly luciferase. See Tusher et al. Proc Natl Acad Sci USA. 2001; 98:5116-5121. As a control, an LM02 3′UTR mutant was created using mutant PCR primers 3 and 4 and then outer primers 1 and 2. The resulting fragment was also placed into the XhoI and XbaI sites of pL/SV40/GL3. In this LM02 3′UTR mutant, the seed match predicted to bind to nucleotides 2-8 of miR-223 is converted from 5′AACUGAC 3′ to 5′AACAGUC 3′. To create a miR-223 expression vector, a ˜350 nucleotide-long fragment of pri-miR-223, encompassing the pre-miRNA stem loop in its middle, was PCR-amplified from genomic BJAB DNA with primers 6 and 7 (SEQ ID NOs. 6 and 7) and ligated into the XhoI and XbaI sites of the pL/CMV/eGFP vector. This pL/CMV/eGFP vector was generated by ligating a fragment containing the CMV promoter and the EGFP ORF into the BamHI and XhoI sites of the previously described lentiviral backbone pL. See Tusher et al. Proc Natl Acad Sci USA. 2001; 98:5116-5121. The expression of miR-223 from the 3′UTR of EGFP in the resulting vector was confirmed by Taqman real time PCR in transfected 293T cells.
For luciferase indicator assays, 293T cells plated in 24 well plates were transfected using FUGENE6 as follows:
TABLE 1
Luciferase indicator assay compositions
2.5 ng pL/SV40/GL3 2.5 ng pL/SV40/GL3/ 2.5 ng pL/SV40/GL3/
2.5 ng pL/SV40/RLuc LMO2 LMO2 seed
0.4 μg pL/CMV/ UTR mut
eGFP/miR-223 2.5 ng pL/SV40/RLuc 2.5 ng pL/SV40/RLuc
2.5 ng pL/SV40/GL3 0.4 μg pL/CMV/ 0.4 μg pL/CMV/eGFP/
2.5 ng pL/SV40/RLuc eGFP/miR-223 miR-223
0.4 μg pL/CMV/eGFP 2.5 ng pL/SV40/ 2.5 ng pL/SV40/GL3/
GL3/LMO2 LMO2 seed
UTR mut
2.5 ng pL/SV40/RLuc 2.5 ng pL/SV40/RLuc
0.4 μg pL/CMV/eGFP 0.4 μg pL/CMV/eGFP
Reporter expression was evaluated by dual luciferase assays (Promega) 48 hours post-transfection. Firefly Luciferase (GL3) to internal control Renilla Luciferase (RLuc) ratios from 293T cells transfected with pL/CMV/eGFP/miR-223 were divided by those obtained from 293T transfected with the pL/CMV/eGFP vector control. The average and standard deviation were taken across five experiments for the pL/SV40/gl3 empty, LM02, and LM02 mutant vectors.
Firefly Luciferase (GL3) activity readings of the PRDM1 3′UTR construct were divided by internal control Renilla Luciferase (RLuc) activity readings. The average and standard deviation of these ratios across three experiments were calculated and scaled relative to the empty vector (pL/CMV/eGFP) transfection.
TABLE 2
LM02 primer sequences
Primer
SEQ ID description Sequence (5′ → 3′)
1543 LMO2 3′UTR, FW ATATCTCGAGGCCCGAGTCCCCGGGCATCTTTGG
1544 LMO2 3′UTR, REV ATATATCTAGACTACACACGACAAATACTTTG
1545 LMO2 3′UTR seed CAGCCCATCCATAGTAACAGTCATGATTAGCAGAAGAAAGG
mutant, FW
1546 LMO2 3′UTR seed CCTTTCTTCTGCTAATCATGACTGTTACTATGGATGGGCTG
mutant, REV
1547 pri-mir-223, FW ATATCTCGAGGGTCACATCTCCCAGGAAGATC
1548 pri-mir-223, REV ATATATCTAGAAGCACTCTCATGGTGTGTGTAG
PRDM1
The PRDM1 3′UTR was PCR-amplified from BJAB genomic DNA in two reactions using primer pairs 7 and 8 in one reaction, and 9 and 10 in another (SEQ ID NOs: 7 to 10, respectively). The two fragments were then ligated together into the XhoI and NotI sites of pL/SV40/GL3 to generate the entire PRDM1 3′UTR. microRNA expression vectors were created as described above for mir-9-2, mir-30b, and mir-30d with the primers listed below. Again, over-expression from the 3′UTR of EGFP in the resulting vector was confirmed by Taqman real-time PCR in transfected 293T cells. For the PRDM1 luciferase assays, we used the sequences listed below to PCR-mutate the seed sequence of the microRNA expression vectors rather than the PRDM1 3′UTR, which had numerous seed sequence binding sites.
TABLE 3
PRDM1 primer sequences
Primer
SEQ ID: description sequence
1549 PRDM1 3′UTR piece 1, FW AGAGACTCGAGGATTTTCAGAAAACACTTATTT
1550 PRDM1 3′UTR piece 1, REV TTGCTTCTCTAGAGGAGAAAC
1551 PRDM1 3′UTR piece 2, FW GTTTCTCCTCTAGAGAAGCAA
1552 PRDM1 3′UTR piece 2, REV AGAGAGCGGCCGCAGGGGAGAGACAAATTGCATTG
1553 pri-mir-9-2, FW AGAGACTCGAGATAAAAGGAGGAATCTTAAG
1554 pri-mir-9-2, REV AGAGAGCGGCCGCGAAAAAAACAAAACAAAAACAA
1555 pri-mir-30b, FW AGAGAGCGGCCGCCCGATTGAGTCTTGCCTCAT
1556 pri-mir-30b, REV AGAGAGAATTCAATGGTCTCACATTTCCAAC
1557 pri-mir-30d, FW AGAGAGCGGCCGCATGTCACAGCTATTGTTCAG
1558 pri-mir-30d, REV AGAGAGAATTCGCAGTAAAAGAATGCAGCTA
1559 pri-mir-9-2 seed mutant, FW GGAAGCGAGTTGTTATCTATGCTTATCTAGCTGTATGAGT
1560 pri-mir-9-2 seed mutant, REV ACTCATACAGCTAGATAAGCATAGATAACAACTCGCTTCC
1561 pri-mir-30b seed mutant, FW ACCAAGTTTCAGTTCATGTTAAGATCCTACACTCAGCTGT
1562 pri-mir-30b seed mutant, REV ACAGCTGAGTGTAGGATCTTAACATGAACTGAAACTTGGT
1563 pri-mir-30d seed mutant, FW CAGAAAGTCTGTTGTTGTTAAGATCCCCGACTGGAAGCTG
1564 pri-mir-30d seed mutant, REV CAGCTTCCAGTCGGGGATCTTAACAACAACAGACTTTCTG
Luciferase assays were carried out in a manner similar to those described for LMO2.
IgVH Mutation Status of Chronic Lymphocytic Leukemia Samples
IgVH mutation status was determined as described in Volkheimer et al. (Blood. 2007; 109:1559-1567) using genomic DNA. In brief, genomic DNA was isolated from purified CLL cells and isolated using the GenElute Mammalian DNA extraction Kit from Sigma (St. Louis, Mo.) according to the manufacturer's instructions. DNA was amplified using nested PCR primers. PCR products were electrophoresed, purified, and sequenced using an automated DNA sequencer (Applied Biosystems, Foster City, Calif.) with the BigDye Terminator kit (Perkin Elmer, Boston, Mass.). Forward and reverse sequences were aligned into a single resolved sequence using Sequencher 4.1 software (Gene Codes Corporation, Ann Arbor, Mich.), and then aligned with germline sequences derived from DNA Plot on the V BASE directory website (http://vbase.mrc-cpe.cam.ac.uk/). The percent sequence identity was calculated by dividing the number of mutations from FR1 to FR3 by the total number of nucleotides in this region. Samples were considered somatically mutated if they had greater than 2% mutations in this region.
Example 2 Mature B Cell Stages Display Characteristic Patterns of MicroRNA Expression Mature B cell subsets can be defined by the expression of surface CD19, IgD, CD38, and CD27, and were obtained by fluorescence activated cell sorting of tonsils from young individuals undergoing routine tonsillectomy. See FIGS. 1B and 1C. Cells were previously gated on CD19 positive cells. Naive and memory B cells were distinguished from germinal center and plasma cells based on surface CD38 and IgD expression.
To determine whether mature B cell subsets had unique patterns of microRNA (miRNA) expression, we used a 384-well multiplexed real time polymerase chain reaction (RT-PCR) assay (Applied Biosystems) that allowed measurement of all 365 miRNAs in miRBase 9.2. See Chen et al. Nucleic Acids Res. 2005; 33:e179; and He et al. Nature. 2007; 447:1130-1134. We detected a total of 113 unique miRNAs in the B cell populations. See Table 4. This detection frequency compares favorably to the identification of 71 unique miRNAs (45 miRNAs with more than one clone) through the examination of 3101 sequences cloned from unselected CD19-positive mature B cells. See Landgraf et al. Cell. 2007; 129:1401-1414. We identified differentially expressed miRNAs in mature B cell subsets using a false discovery rate of less than 5%. See FIGS. 1D, 1F, and 1H. The complete list of assayed miRNAs found to be expressed in the B cell populations is shown in Tables 4 and 5.
In Table 4, normalized expression values for B-cell subsets are shown, along with significance analysis of microarrays q values. Empty microRNA expression cells indicate below-threshold values, defined as RT-QPCR Ct values greater than 36 or undetected. SAM q values greater than 5 were considered non-significant and not displayed.
TABLE 4
MicroRNAs detected in at least one B-cell subset
qNaive qGerminal qGerminal
Germinal vs Center Center
Naïve Center Plasma Cell Memory Germinal vs vs
average average average average Center Plasma Cell Memory
hsa-let-7a 11.7 9.2 11.2 1.4
hsa-let-7b 10.3 9.0 7.7 11.3 0.0
hsa-let-7c 7.3 7.2
hsa-let-7d 9.0 9.0 10.0
hsa-let-7f 10.3 7.6 8.4 10.2 0.0
hsa-let-7g 13.1 10.3 10.5 14.0 0.0 0.0
hsa-miR-100 7.4
hsa-miR-101 8.4 10.0
hsa-miR-103 9.7 11.4 9.8 10.8 0.0 0.0
hsa-miR-106b 11.7 12.5 11.6 12.7
hsa-miR-125a 6.7 8.3
hsa-miR-125b 6.3 7.3
hsa-miR-130b 7.8 9.8 8.6 10.2 3.6 4.5
hsa-miR-132 7.5 8.9
hsa-miR-133b 7.7 7.7 7.2 7.3
hsa-miR-140 10.8 12.0 11.1 12.0 4.7
hsa-miR-141 7.0 6.2
hsa-miR-142-3p 16.9 17.0 15.3 18.0 1.1
hsa-miR-142-5p 11.7 11.0 9.8 13.4 2.1
hsa-miR-146a 10.1 14.0 14.0 14.6 0.0
hsa-miR-146b 9.2 9.2 8.1 10.5 2.1
hsa-miR-148a 6.7 9.4 11.9 9.0 0.0
hsa-miR-148b 6.4 8.5
hsa-miR-151 8.2 9.6 6.6 9.0 1.5 0.0
hsa-miR-152 6.4 9.7 6.6
hsa-miR-155 14.2 14.6 15.0 15.6
hsa-miR-15a 10.2 10.1 7.8 10.8 2.0
hsa-miR-15b 11.2 14.0 12.6 12.9 0.0 2.8
hsa-miR-16 16.7 17.3 17.1 17.1
hsa-miR-17-3p 9.1
hsa-miR-17-5p 8.8 11.5 8.9 9.4 0.0 0.0 0.0
hsa-miR-181b 9.0 11.7 10.8 9.3 0.0 0.0
hsa-miR-181d 8.2 10.6 8.5 9.0 0.0 1.1
hsa-miR-182 8.7 8.7
hsa-miR-186 8.8 9.2 8.7 9.7
hsa-miR-18a 5.7 8.9 6.8 7.4 0.0 0.0
hsa-miR-191 11.9 12.7 12.4 13.3
hsa-miR-192 9.1 9.0 8.8 10.1
hsa-miR-193b 6.6
hsa-miR-194 6.8 7.4 4.9 9.5 2.0 4.7
hsa-miR-195 10.3 8.7 8.8 9.2
hsa-miR-197 9.7 10.5 8.7 11.0 0.0
hsa-miR-199a* 7.0
hsa-miR-19a 9.7 12.8 11.9 11.1 0.0
hsa-miR-19b 14.0 15.6 14.9 15.0 0.8
hsa-miR-200a 6.5 6.8
hsa-miR-200b 7.5
hsa-miR-200c 9.0 9.6 9.7
hsa-miR-203 7.9 7.6
hsa-miR-20a 12.5 14.9 12.2 13.8 0.0 0.0
hsa-miR-20b 8.4 11.0 8.0 8.5 0.0 1.1 0.0
hsa-miR-21 12.6 13.0 11.2 15.1 2.8 2.1
hsa-miR-210 9.2 11.1 10.7 10.2 0.8
hsa-miR-214 7.6
hsa-miR-22 8.3 7.4
hsa-miR-221 6.9
hsa-miR-222 11.4 11.6 7.8 12.7 0.0 4.7
hsa-miR-223 13.7 10.4 9.6 14.6 0.0 0.0
hsa-miR-23a 8.7
hsa-miR-23b 6.2
hsa-miR-24 11.7 11.8 12.8 13.4 2.1
hsa-miR-25 9.7 10.8 8.8 11.2 2.8
hsa-miR-26a 14.7 13.6 14.2 16.3 0.0
hsa-miR-26b 12.6 12.1 12.0 14.1 3.4
hsa-miR-27a 6.5 11.1 0.0
hsa-miR-27b 7.2
hsa-miR-28 9.4 13.3 8.4 10.3 0.0 0.0 0.0
hsa-miR-296 6.9 8.1 6.6 7.9 0.0 2.0
hsa-miR-29a 13.4 11.7 13.5 15.0 0.0
hsa-miR-29c 11.2 8.7 12.3 13.3 1.5 2.6 0.0
hsa-miR-301 6.0 9.6 7.7 7.9 0.0 0.0
hsa-miR-30a-3p 8.2
hsa-miR-30a-5p 11.9 12.6 11.3 13.0 2.0
hsa-miR-30b 11.7 12.6 11.2 13.0 2.8
hsa-miR-30c 13.0 13.9 12.7 14.3 4.5
hsa-miR-30d 10.8 11.9 10.1 12.1 3.8 0.0
hsa-miR-30e-3p 8.3 9.0 6.8 9.9 0.0
hsa-miR-30e-5p 12.5 8.1 10.2 14.8 0.0
hsa-miR-31 9.0
hsa-miR-32 8.4 7.0 10.5
hsa-miR-320 10.1 8.2 10.3 10.5 1.5 2.6 0.0
hsa-miR-324-3p 8.8 9.9 9.0 9.5
hsa-miR-324-5p 8.0 7.8
hsa-miR-328 8.1 8.6 7.4 8.7 2.8
hsa-miR-331 9.4 12.6 12.0 10.8 0.0 2.6
hsa-miR-335 6.3 6.9
hsa-miR-339 7.3 5.7 8.9
hsa-miR-342 12.7 12.5 10.6 13.1 0.0
hsa-miR-345 6.8 7.1 7.8 8.6 3.4
hsa-miR-361 8.3
hsa-miR-365 6.0 7.8 8.3 8.4 0.0
hsa-miR-374 8.0 8.9 10.4 9.8
hsa-miR-423 8.0 9.7 7.6 8.7 0.0 0.0
hsa-miR-425 6.9
hsa-miR-425-5p 8.7 11.6 9.7 10.2 0.0 0.0
hsa-miR-484 11.5 12.3 12.9 12.0
hsa-miR-486 7.0 8.8 9.9 9.6 3.8
hsa-miR-532 7.0 7.8
hsa-miR-545 5.5 6.3
hsa-miR-572 7.7 6.8
hsa-miR-629 6.7 7.3 7.2 9.0 1.4
hsa-miR-646 6.0 7.3
hsa-miR-650 8.8 11.4 8.0
hsa-miR-659 7.6 6.0 6.8
hsa-miR-660 9.5 8.6 10.1
hsa-miR-7 6.1 5.8
hsa-miR-9 7.8 6.8 6.2 2.8
hsa-miR-9* 6.4 6.5
hsa-miR-92 14.5 15.8 14.2 15.5 3.6 0.0
hsa-miR-93 11.5 14.6 10.5 12.3 0.0 0.0 0.0
hsa-miR-98 6.3 5.0 5.0 7.8 2.1
hsa-miR-99a 7.3
hsa-miR-99b 6.8
TABLE 5
miRNAs measured using the multiplex RT-PCR assay, but not consistently
detected in any B-cell population
hsa-let-7e hsa-miR-383 hsa-miR-376a hsa-miR-566
hsa-miR-I0a hsa-miR-198 hsa-miR-376b hsa-miR-551b
hsa-miR-I0b hsa-miR-224 hsa-miR-380-5p hsa-miR-569
hsa-miR-34a hsa-miR-299-5p hsa-miR-410 hsa-miR-570
hsa-miR-34b hsa-miR-409-5p hsa-miR-412 hsa-miR-548a
hsa-miR-34c hsa-miR-432 hsa-miR-432 hsa-miR-586
hsa-miR-107 hsa-miR-433 hsa-miR-512-5p hsa-miR-587
hsa-miR-181c hsa-miR-485-5p hsa-miR-199a hsa-miR-548b
hsa-miR-215 hsa-miR-489 hsa-miR-199b hsa-miR-588
hsa-miR-218 hsa-miR-494 hsa-miR-219 hsa-miR-589
hsa-miR-372 hsa-miR-506 hsa-miR-323 hsa-miR-550
hsa-miR-375 hsa-miR-508 hsa-miR-338 hsa-miR-591
hsa-miR-378 hsa-miR-521 hsa-miR-368 hsa-miR-593
hsa-miR-137 hsa-miR-134 hsa-miR-373 hsa-miR-596
hsa-miR-200a hsa-miR-147 hsa-miR-373 hsa-miR-597
hsa-miR-I hsa-miR-149 hsa-miR-382 hsa-miR-622
hsa-miR-183 hsa-miR-153 hsa-miR-424 hsa-miR-599
hsa-miR-302a hsa-miR-187 hsa-miR-448 hsa-miR-600
hsa-miR-302c hsa-miR-190 hsa-miR-450 hsa-miR-624
hsa-miR-302d hsa-miR-193a hsa-miR-451 hsa-miR-601
hsa-miR-367 hsa-miR-196a hsa-miR-452 hsa-miR-626
hsa-miR-369-5p hsa-miR-196b hsa-miR-452 hsa-miR-548d
hsa-miR-449 hsa-miR-205 hsa-miR-453 hsa-miR-639
hsa-miR-497 hsa-miR-208 hsa-miR-485-3p hsa-miR-613
hsa-miR-501 hsa-miR-213 hsa-miR-488 hsa-miR-614
hsa-miR-509 hsa-miR-220 hsa-miR-490 hsa-miR-615
hsa-miR-510 hsa-miR-325 hsa-miR-492 hsa-miR-616
hsa-miR-511 hsa-miR-326 hsa-miR-493 hsa-miR-548c
hsa-miR-514 hsa-miR-337 hsa-miR-503 hsa-miR-617
hsa-miR-515-3p hsa-miR-340 hsa-miR-504 hsa-miR-642
hsa-miR-515-5p hsa-miR-380-3p hsa-miR-505 hsa-miR-618
hsa-miR-517a hsa-miR-422b hsa-miR-507 hsa-miR-644
hsa-miR-517b hsa-miR-422a hsa-miR-513 hsa-miR-647
hsa-miR-517c hsa-miR-429 hsa-miR-516-5p hsa-miR-649
hsa-miR-518a hsa-miR-491 hsa-miR-517 hsa-miR-661
hsa-miR-518b hsa-miR-496 hsa-miR-518c hsa-miR-662
hsa-miR-518c hsa-miR-500 hsa-miR-518f hsa-miR-449b
hsa-miR-518d hsa-miR-502 hsa-miR-519b hsa-miR-653
hsa-miR-518e hsa-miR-105 hsa-miR-519c hsa-miR-411
hsa-miR-520a hsa-miR-122a hsa-miR-519d hsa-miR-654
hsa-miR-520b hsa-miR-124a hsa-miR-51ge hsa-miR-575
hsa-miR-520c hsa-miR-126 hsa-miR-522 hsa-miR-576
hsa-miR-520d hsa-miR-128b hsa-miR-523 hsa-miR-578
hsa-miR-520e hsa-miR-129 hsa-miR-524 hsa-miR-579
hsa-miR-520f hsa-miR-130a hsa-miR-526b hsa-miR-580
hsa-miR-520g hsa-miR-139 hsa-miR-96 hsa-miR-585
hsa-miR-520h hsa-miR-143 hsa-miR-651 hsa-miR-
512-3p
hsa-miR-95 hsa-miR-145 hsa-miR-376a hsa-miR-631
hsa-miR-126 hsa-miR-182 hsa-miR-542-5p hsa-miR-363
hsa-miR-127 hsa-miR-185 hsa-miR-544 hsa-miR-487b
hsa-miR-133a hsa-miR-189 hsa-miR-656 hsa-miR-645
hsa-miR-135a hsa-miR-18b hsa-miR-549 hsa-miR-556
hsa-miR-135b hsa-miR-202 hsa-miR-657 hsa-miR-558
hsa-miR-184 hsa-miR-202 hsa-miR-658 hsa-miR-627
hsa-miR-204 hsa-miR-299-3p hsa-miR-652 hsa-miR-630
hsa-miR-206 hsa-miR-302a hsa-miR-551a hsa-miR-603
hsa-miR-211 hsa-miR-302b hsa-miR-552 hsa-miR-606
hsa-miR-216 hsa-miR-302b hsa-miR-553 hsa-miR-607
hsa-miR-217 hsa-miR-302c hsa-miR-554 hsa-miR-608
hsa-miR-330 hsa-miR-329 hsa-miR-555 hsa-miR-609
hsa-miR-371 hsa-miR-33 hsa-miR-562 hsa-miR-633
hsa-miR-379 hsa-miR-362 hsa-miR-563 hsa-miR-565*
hsa-miR-381 hsa-miR-369-3p hsa-miR-564 hsa-miR-594*
miRNAs marked with * were not used in analyses because they have been reclassified as non-miRNAs.
The B cell subsets were profiled for gene expression at the whole genome level, as described previously. See Dave et al. N Engl J Med. 2004; 351:2159-2169. At each stage, we identified differentially expressed genes as those genes with a mean two-fold difference in expression and a false-discovery rate of less than 1%. See FIGS. 1E, 1G, and 1I. Genes that we found to be differentially expressed in each stage-transition were consistent with previous studies that examined gene expression in B cell subsets using microarrays with fewer probes, an overlap that was found to be highly statistically significant (P<0.001, chi-squared test). See, e.g., Klein et al. Proc Natl Acad Sci USA. 2003; 100:2639-2644; and Shaffer et al. Immunity. 2001; 15: 375-385.
In the naïve→germinal center (GC) B cell transition, we identified 32 miRNAs that were differentially expressed. Interestingly, all but 4 miRNAs were found to be expressed more highly in GC cells than in naive B cells. See FIG. 1D. We confirmed the mRNA expression patterns of several genes that are known to be differentially expressed in the transition including BCL6, MME, MYBL1, as well as LM02. See FIG. 1E. LM02 was found to be expressed more highly in germinal center B cells compared to both naive B cells and memory B cells. See FIGS. 1E and 1I. In the GC→plasma cell transition, we found 33 miRNAs that were differentially expressed. Once again, we noted a striking asymmetry, with all but 2 miRNAs found to be expressed highly in GC cells, but down-regulated in plasma cells. See FIG. 1F. We also confirmed that the plasma cell-specific genes, PRDM1 (FIG. 1G), XBP1 and IRF4 were highly differentially expressed in our experiments. In the GC→memory B cell transition, there was a preponderance of the 27 significant miRNAs expressed at higher levels in memory cells, See FIG. 1H. Five miRNAs were expressed highly in GC cells compared to all the other B cell types. These included 3 members of the miR-17˜92 cluster (miR-17-5p, miR-20b, miR-93), as well as miR-28 and miR-181b.
The expression pattern of all the miRNAs that were measurable in at least one of the B cell subsets is summarized in FIG. 5. Notably, there were no differences in the expression of genes involved in miRNA processing, including DICER1, DROSHA, XP05 (exportin5), EIF2C2 (ag02) and DGCR8, among the B cell subsets. See FIG. 1J.
Separately, we examined the expression of predicted target genes of differentially expressed microRNAs. We also found that predicted mRNA target genes of microRNAs expressed highly in GC cells were expressed at lower levels in GC cells compared to other stages. See FIG. 6 and Appendix A. FIG. 6A shows density plots of the expression frequency of predicted mRNA targets of microRNAs expressed highly in germinal center B cells compared to naive cells. mRNAs from FIG. 1E that were also predicted targets of the miRNAs (N=830) were plotted for both naive cells and germinal center B cells. The depicted p-value was calculated using a 1-sided Kolmogorov-Smirnov test. FIG. 6B shows density plots of the expression frequency of predicted mRNA targets of miRNAs expressed highly in germinal center B cells compared to plasma cells. mRNAs from FIG. 1G that were also predicted targets of the miRNAs (N=1098) were plotted for both plasma cells and germinal center B cells. The depicted p-value was calculated using a 1-sided Kolmogorov-Smirnov test. FIG. 6C shows density plots of the expression frequency of predicted mRNA targets of miRNAs expressed highly in the germinal center B cells compared to memory B cells. mRNAs from FIG. 1I that were also predicted targets of the miRNAs (N=269) were plotted for both naive cells and germinal center B cells. The depicted p-value was calculated using a 1-sided Kolmogorov-Smirnov test.
In the naïve to germinal center transition (see FIG. 6A), genes with at least a two-fold change in expression were analyzed by SAM using a false discovery rate of less than 1% (Larsson et. al, BMC Bioinformatics. 2005; 6:129), depicted in FIG. 1E. From that list of significantly differentially expressed genes, we determined those that were predicted targets of miRNAs (defined as genes with 3′UTR sequence complementarity to microRNA nucleotides 2-7) that were expressed more highly in germinal center cells. The gene expression distribution of those mRNA targets was graphed for naïve B Cells (blue curve) and germinal center B cells (orange curve). Thus, in FIG. 6A, we show genes differentially expressed between naïve and germinal center B cells that are also predicted targets of miRNA expressed more highly in germinal center B cells. We observe that the expression of these microRNA target genes is lower in germinal center B cells compared to naïve B cells.
A similar analysis was carried out for the transitions from germinal center to plasma cell (FIG. 6B) and germinal center to memory cell (FIG. 6C). In all three cases examined, the distribution of germinal center miRNA target gene expression is statistically significantly lower (leftward shift of orange curve), which suggests a possible gene regulatory role for the miRNAs that are more highly expressed in germinal center cells. We note that the observed lower expression of the miRNA target genes could, however, also be caused by other factors such as downstream effects of particular transcription factors. The complete set of genes plotted in FIG. 6 is listed in Appendix A.
Finally, we found that a higher proportion of differentially expressed transcription factors are predicted microRNA targets. See FIG. 7. Table 6 shows differentially expressed transcription factors, with the average level of expression for each in naïve, germinal center, plasma, and memory cells. Transcription factors for which the significance analysis showed a q value of 0 are indicated.
TABLE 6
Differentially-expressed transcription factors
Naïve GC
Germinal Plasma vs GC vs
Naïve Center Cell Memory GC: q vs PC: q Mem: q
ID NAME average average average average value value value
1 AHR 8.9 10.33 8.36 10.38 0 0
2 APBB2 6.86 7.87 7.35 6.39 0 0
3 ARID1A 12.3 12.22 11.11 12.17 0
4 ARID3A 8.03 6.9 9.81 8.76 0 0 0
5 ARNTL 9.47 8.4 8.23 9.7 0 0
6 ATF7 7.83 8.97 8.23 8.44 0
7 BACH1 10.68 9.97 11.76 10.59 0
8 BHLHB3 11.11 8.05 13.26 13.26 0 0 0
9 BPTF 12.23 12.46 11.34 11.86 0
10 BTG1 15.88 14.95 13.94 15.28 0
11 CASP8AP2 10.17 10.55 9.44 10.22 0
12 CBL 11.07 11.69 10.26 11.04 0
13 CBX4 12.23 12.36 13.69 12.68 0
14 CCNE1 7.86 9.18 8.87 7.66 0 0
15 CDH1 5.85 3.85 10.26 4.91 0
16 CITED2 9.11 9.88 12.56 9.22 0
17 CLOCK 9.85 7.68 9.91 9.62 0 0 0
18 CREB5 4.91 5.46 7.24 6.95 0
19 CREBL1 7.53 6.97 8.06 8.09 0
20 CREBL2 10.18 9.95 11.1 10.79 0
21 CSDA 12.12 8.59 10.1 8.98 0 0
22 DEK 12.73 14.09 10.29 13.15 0 0
23 DLX2 6.08 4.66 7.04 7.08 0 0
24 DR1 11.83 11.97 10.65 12.1 0
25 DTX1 10.98 11.6 10.55 9.62 0 0
26 DYRK1B 6.32 5.92 7.61 4.96 0
27 E2F1 7.01 8.37 7.95 7.21 0 0
28 EGR1 11.92 13.24 13.77 12.75 0
29 EGR2 10.21 10.43 8.07 10.4 0
30 EGR3 11.59 9.35 8.24 10.62 0 0 0
31 ELF1 12.39 12.7 11.34 12.55 0
32 ELL 7.12 7.83 9.17 7.59 0
33 ELL2 9.25 8.23 13.6 9.27 0
34 EPAS1 7.1 7.02 8.39 7.5 0
35 ETS1 14.25 14.4 12.62 14.17 0
36 ETS2 6.37 7.5 5.66 4.9 0 0 0
37 ETV1 8.19 6.9 7.38 7.06 0
38 ETV4 5.99 5.42 7.15 6.09 0
39 ETV6 10.36 7.14 8.89 10.43 0 0 0
40 FHL2 4.82 7.77 6.82 5.65 0 0
41 FLNA 9.95 9.2 8.57 11.03 0
42 FOS 12.35 11.25 13.34 11.23 0 0
43 FOSB 10.13 7.21 11.05 10.79 0 0 0
44 FOXC1 6.81 5.84 7.39 6.59 0
45 FOXF2 7.05 4.83 6.37 5.84 0
46 FOXJ2 10.01 8.38 9.97 9.85 0 0 0
47 FOXK2 8.94 8.4 9.63 8.71 0
48 GATA3 8.05 7.76 8.51 9.6 0
49 GATA6 7.08 5.41 7.59 6.26 0 0
50 GCN5L2 10.18 8.97 8.9 10.36 0 0
51 GLI2 6.93 7.26 7.52 6.09 0
52 GPX3 6.72 5.92 7.61 5.58 0
53 HCLS1 13.94 14.42 13.12 13.92 0
54 HIPK2 9.36 8.66 11.04 10.85 0 0
55 HMGA1 11.26 12.47 10.36 10.86 0 0 0
56 HMGB1 15.47 16.36 14.98 14.97 0 0
57 HMGB2 12.96 15.78 12.73 13.36 0 0 0
58 HOXA1 5.36 7.7 7.23 7.64 0
59 HOXA5 7.03 8.32 8.36 7.54 0
60 HOXB4 8.2 7.58 9.27 8.01 0
61 HOXB9 7.4 6.2 8.41 6.87 0
62 HOXC11 6.6 6.4 7.88 6.21 0
63 HOXC8 7.05 5.48 6.88 6.71 0
64 ID4 6.49 5.93 7.79 6.41 0
65 ILF3 11.26 11.94 10.71 10.99 0
66 IRAK1 12.19 11.48 12.8 11.96 0
67 IRF4 11.16 9.28 13.66 10.51 0 0 0
68 ISL1 6.98 5.91 7.6 6.11 0
69 JAZF1 11.93 10.65 7.49 11.31 0 0
70 JMJD1C 12.34 12.34 11.11 12.56 0
71 JUN 12.14 9.34 12.7 12.16 0 0 0
72 JUNB 11.62 9.72 10.84 11.37 0 0
73 KLF11 8.08 5.94 6.88 8.39 0 0
74 KLF2 12.61 9.1 11.7 12.61 0 0 0
75 KLF4 10.04 7.57 9.09 7.49 0 0
76 KLF5 7.08 5.26 6.14 5.07 0
77 KLF6 12.06 11.33 9.24 12.40 0 0
78 KLF7 9.36 8.35 8.88 9.8 0
79 KLF9 9.47 7.34 6.64 9.27 0 0
80 LITAF 12.52 10.18 11.82 12.49 0 0 0
81 LMO1 6.86 4.75 7.16 6.88 0
82 MAF 8.05 9.95 8.25 10.83 0 0
83 MAFB 6.58 7.3 8.56 7.29 0
84 MAML3 8.05 10.85 9.07 7.04 0 0 0
85 MDFIC 12.06 13.67 13.6 13.08 0
86 MEIS2 7.45 7.15 7.56 5.29 0
87 MEN1 10.79 11.33 10.49 10.23 0
88 MITF 7.51 8.05 8.01 6.41 0
89 MTF1 10.08 9.92 8.86 10.5 0
90 MXD1 9.07 7.59 9.62 8.9 0 0 0
91 MXI1 10.63 7.85 11.1 9.42 0 0 0
92 MYB 6.97 8.62 5.61 7.99 0 0
93 MYBL1 9.68 14.13 10.02 9.72 0 0 0
94 MYBL2 9.07 12.41 9.12 8.52 0 0 0
95 NAT14 6.62 7.66 7.33 7.63 0
96 NF1 9.23 10.46 11.21 9.88 0
97 NFAT5 11.71 12.09 10.72 11.77 0
98 NFATC4 6.36 7.92 8.96 5.71 0 0
99 NFIX 8.09 7.43 8.67 7.37 0
100 NFKB1 11.91 11.9 10.7 11.55 0
101 NFYC 10.2 10.27 9.03 10.03 0
102 NR3C2 8.85 5.58 4.91 8.41 0 0
103 NR6A1 9.1 6.03 6.42 7.62 0 0
104 NRIP1 11.79 10.3 10.33 11.20 0
105 PAX3 7.01 5.55 5.01 5.96 0
106 PHF1 11.85 10.69 12.26 11.45 0 0
107 POU3F1 6.08 7.52 6.86 7.52 0
108 POU4F1 5.62 8.85 8.46 7.53 0 0
109 POU4F2 7.09 5.72 7.21 5.1 0 0
110 PPARD 11.23 9.98 10.29 10.69 0
111 PPARG 7.79 7.22 6.74 5.47 0
112 PPARGC1B 6.89 6.09 6.51 7.91 0
113 PRDM1 8.49 9.15 14.1 9.33 0
114 PRDM4 10.48 9.81 9.76 10.83 0
115 PROX1 7.18 6.38 7.89 5.54 0
116 RAN 13.46 14.57 12.39 13.78 0 0
117 RSF1 10.05 9.1 9.56 10.18 0
118 RUNX1T1 7.37 5.95 6.63 6.34 0
119 RUNX2 8.75 7.91 10.22 9.75 0 0
120 RUNX3 11.8 11.13 10.26 12.47 0
121 RXRA 9.07 7.15 8.81 8.32 0 0 0
122 SAP30 8.22 9.91 9.28 8.33 0 0
123 SCMH1 8.48 9.69 9.65 8.62 0 0
124 SCML1 10.32 8.48 7.81 7.91 0
125 SCML2 10.33 8.76 9.27 8.96 0
126 SF1 11.72 11.44 10.36 11.37 0
127 SIAH2 10.35 12.34 10.57 9.90 0 0 0
128 SLC2A4RG 8.25 8.98 7.4 8.91 0
129 SMAD1 5.91 8.1 6.34 7.33 0 0
130 SMAD2 10.45 11.57 10.8 10.98 0
131 SMAD3 10.72 9.23 7.74 10.54 0 0 0
132 SMARCA2 11.14 10.12 10.79 11.19 0 0
133 SOLH 8.78 7.88 8.7 9.03 0
134 SOX4 8.99 9.14 9.17 10.2 0
135 SOX5 9.3 10.47 7.33 9.08 0 0 0
136 SOX9 6.72 8.42 8.18 7.03 0 0
137 SP4 11.26 10.14 9.58 11.75 0 0
138 SRCAP 8.58 8.06 6.96 8.38 0
139 SREBF1 8.23 8.36 6.56 5.89 0 0
140 STAT5B 10.35 10.95 9.73 10.7 0
141 SUFU 7.12 6.03 7.84 7.62 0 0
142 SUPT16H 11.12 11.35 9.6 10.71 0
143 SUPT3H 7.77 6.3 8.05 8.35 0 0 0
144 TARDBP 12.31 12.72 11.49 11.91 0
145 TBP 10.55 10.14 9.09 10.38 0
146 TBX3 5.67 6.05 7.89 5.53 0
147 TCEA1 13.31 15 14.05 13.6 0 0
148 TCERG1 12.29 12.5 11.46 12.04 0
149 TCF7 10.21 10.82 9.97 12.61 0
150 TFAP2A 6.7 5.54 8.26 6.88 0
151 TFAP4 7.28 5.29 5.42 5.13 0
152 TFDP1 10.95 12.9 10.49 11.36 0 0 0
153 TFEB 11.56 11.15 9.49 11.77 0
154 THRA 7.41 7.26 8.48 7.05 0
155 THRB 7.45 7.51 6.11 6.29 0 0
156 TLE1 9.19 7.74 9.63 9.13 0 0 0
157 TMF1 10.76 9.68 10.71 10.42 0
158 TSC22D3 13.22 11.26 12.67 13.4 0 0 0
159 UHRF1 9.23 12.22 8.72 8.96 0 0 0
160 VEZF1 10.98 11.45 9.94 10.81 0
161 XBP1 10.1 10.74 15.84 10.65 0
162 YBX1 14.79 15.31 14.2 14.53 0
163 YWHAH 8.94 10.82 9.09 9.36 0 0 0
164 YWHAZ 13.38 13.62 12.61 13.62 0
165 ZFP36L1 14.07 12.89 10.09 13.87 0 0
166 ZHX3 8.29 7.76 7.47 6.66 0
167 ZNF207 12.29 13.51 12.69 12.54 0
168 ZNF217 11.68 10.77 9.73 11.61 0
169 ZNF219 6.13 6.11 8.42 6.48 0
170 ZNF238 13.05 11.27 10.08 12.54 0 0 0
171 ZNF3 8.86 7.27 8.75 8.89 0 0 0
172 ZNF367 7.97 10.99 8.04 8.22 0 0 0
173 ZNF398 10.39 9.28 10.47 10.25 0 0
Example 3 MiR-223 Regulates LM02 in the Naive˜Germinal Center and Germinal Center˜Memory Cell Transitions MiR-223 has previously been described as being important in the commitment to myeloid lineage. See Chen et al. Science. 2004; 303:83-86; Johnnidis et al. Nature. 2008; 451:1125-1129. We found miR-223 to be expressed at nearly 8-fold higher levels in both naive and memory cells compared to GC cells. This miRNA has a highly conserved sequence complementarity to the 3 ′UTR of 2 transcription factors that are expressed highly during GC cell differentiation: LM02 (FIG. 2A) and MYBL1 (FIG. 8A). Higher expression of miR-223 in the naive B cell stage could inhibit the untimely expression of these transcription factors until the cell is ready to undergo the germinal center reaction.
We evaluated the effects of miR-223 expression on its predicted target gene, LM02, by transfecting precursors of miR-223 into a cell line derived from GC cell lymphoma cells (B1AB). Over-expression of miR-223 resulted in a consistent down-regulation of LM02 at the transcript level compared to a transfection with a scrambled control with no sequence complementarity to the human genome. See FIG. 2B. In that figure, the blue bars depict expression of LM02 24 hours after transfection with a scrambled control that does not possess complementarity to the human genome. The orange bars depict the expression of LM02 24 hours after transfection with a precursor for miR-223. The expression of LM02 was consistently lower in the cells treated with the miR-223 precursor, and the results were statistically significant (P<0.05 in all cases, student's t-test). There was no effect on the expression of a non-target control, beta-2 micro globulin in these experiments (data not shown). Over-expression of miR-223 also resulted in a consistent down-regulation of LM02 at the protein level compared to a transfection with a scrambled control with no sequence complementarity to the human genome. See FIG. 2C. We quantified the results of 3 separate experiments examining LM02 protein expression and found consistent down-regulation of LM02 in cells treated with miR-223 compared to cells transfected with scrambled controls. See FIG. 2D. Those results that were statistically significant (P<0.05, student's t-test). The extent of down-regulation of LM02 mRNA and protein by miR-223 was comparable, suggesting that miR-223 regulation of LM02 occurs predominantly at the mRNA level. Similarly, over-expression of miR-223 resulted in a down-regulation of MYBL1 transcripts. See FIG. 8B. In that figure, the blue bars depict expression of MYBL1 mRNA 24 hours after transfection with a scrambled control that does not possess complementarity to the human genome. The orange bars depict the expression of Mybl1 24 hours after transfection with a precursor for miR-223. The expression of LM02 was consistently lower in the cells treated with the miR-223 precursor (P<0.05 in all cases).
As additional validation, we investigated whether the miR-223 had a direct effect on LM02 by cloning the 3′UTR sequence of LM02 3′ to the firefly luciferase ORF (Fluc). See Gottwein et al. Nature. 2007; 450:1096-1099. The resulting constructs and the unmodified vector were co-transfected into 293T cells along with a Renilla luciferase internal control and pL-CMV-eGFP constructs expressing either no miRNA or miR-223. Fluc expression from constructs bearing LM02 3′ UTR sequences were differentially down-regulated by miR-223 compared to those with mutated seed sequences; the seed sequence mutant construct had consistently diminished miR-223 repression compared to the wild-type construct in 5 separate experiments. See FIG. 2E. Those results were statistically significant (P<0.05, student's t-test). These observations provide evidence for an inhibitory role for miR-223 in regulating the transcription factor LM02.
Activation of LM02 has been associated with the development of leukemia in patients undergoing gene therapy. Hacein-Bey-Abina et al. Science. 2003; 302:415-419. On the other hand, higher expression of PRDM1 alone is sufficient to induce plasma cell-differentiation. Turner et al. Cell. 1994; 77:297-306. Inappropriate expression of such genes must be effectively turned off for a cell to maintain its state. This mode of regulation is reflected in the effects of miR-223, miR-9 and miR-30, which turn off the inappropriate expression of LM02 and PRDM1 and might promote state maintenance and inhibition of lymphomagenesis.
On the other hand, our data also identify a number of instances in which miRNAs are co-expressed with their predicted targets. It is possible that such interactions within the cell help to stabilize a defined expression level by dampening fluctuations. For example, in GC cells, we found that miR-181b was strongly co-expressed with its predicted target, BCL6. Such interactions could also be important in B cell stage maintenance and curbing the oncogenic potential of genes involved in B cell differentiation. See Cattoretti et al. Cancer Cell. 2005; 7:445-455; Dorsett et al. Immunity. 2008; 28:630-638.
Example 4 MiR-9 and the miR-30 Family Regulate PRDM1 (blimp-1) in the Germinal Center→Plasma Cell Transition In the GC→plasma cell transition, we observed that several members of the miR-30 family were expressed at 2-fold or higher levels in GC cells. See FIG. 1F. The transcription factor PRDM1 is an essential regulator of plasma cell differentiation. Martins et al. Annu Rev Immunol. 2008; 26:133-169. The miR-30 family comprises 5 members (miR-30a, 30b, 30c, 30d and 30e), of which 4 (all except 30e) were found to be expressed at higher levels in GC cells compared to plasma cells. Control transfection experiments documented good specificity of the RT-PCR probes for individual members of the miR-30 family with no discernible cross-hybridization. See FIG. 9. Three separate transfection experiments using high concentrations of miR-30b precursors are shown in the left panel with measurement of miR-30b and miR-30d. Similar experiments were performed with precursors for miR-30d (right panel).
The 3′UTR of PRDM1 contains 3 highly conserved binding sites complementary to the seed sequence of members of the miR-30 family, as well as 3 binding sites for the seed sequence of miR-9, 2 of which are highly conserved across multiple species. See FIG. 3A. The 3′UTR region of PRDM1 complementary to miR-30 is shown in red. The 3 sites are complementary to nucleotides 2-8 (UTR position 408), nucleotides 1-8 (UTR position 2370) and nucleotides 2-8 (UTR position 2383) on the miRNA, respectively. The 3′UTR region of PRDM1 complementary to miR-9 is shown in green. The 3 sites are complementary to nucleotides 1-7 (UTR position 1459), nucleotides 2-8 (UTR position 2108) and nucleotides 2-8 (UTR position 2323) on the miRNA respectively. These sites are highly conserved across a number of species, with the exception of one miR-9 site (UTR position 1459) that is only present in humans.
To evaluate the effects of the miR-30 family and miR-9 on PRDM1 expression in plasma cells, we introduced precursors of miR-9, miR-30b and miR-30d into the U266 multiple myeloma (plasma cell) cell line. Overexpression of miR-30 family members miR-30b and miR-30d, as well as miR-9, had no effect on PRDM1 at the mRNA level. See FIG. 3B. In that figure, the blue bars depict expression of PRDM1 24 hours after transfection with a scrambled control with no complementarity to the human genome. The magenta bars depict the expression of PRDM1 24 hours after transfection with a hairpin precursor for miR-30b, while the red bars depict the expression of PRDM1 24 hours after transfection with a hairpin precursor for miR-30d. The green bars depict the expression of PRDM1 24 hours after transfection with a hairpin precursor for miR-9. By contrast, there was a consistent down-regulation of PRDM1 at the protein level. See FIGS. 3C and 3D. Those results were statistically significant in each case (P<0.05, student's t-test), except for the transfections with the precursor to miR-9 (P=0.08, student's t-test). Overexpression of each of those microRNAs had an average knockdown effect of around 40%. These exclusively post-transcriptional effects of miR-9 and miR-30 on PRDM1 expression are consistent with one mechanism of miRNA regulation that has been described previously in other systems. See, e.g., O'Donnell et al. Nature. 2005; 435:839-843; Gottwein et al. Nature. 2007; 450:1096-1099; Selbach et al. Nature. 2008; 455:58-63; and Back et al. Nature. 2008; 455:64-71. There was no effect on the expression of a non-target control (Actin).
Additionally, luciferase reporter activity of the PRDM1 3′UTR construct was decreased by overexpression of miR-9, miR-30b, and miR-30d, but not their respective seed sequence mutants. See FIG. 3E, which shows the average of three experiments. The down-regulation of the luciferase reporter signal and its restoration in the mutant constructs was found to be statistically significant in each of the 3 microRNAs: miR-9, miR-30b and miR-30d (P<0.05, student's t-test). The luciferase reporter activity level was rescued to the activity level of the empty vector control when the seed sequence of the microRNAs was mutated.
The combined effect of 5 different microRNA species (miR-30a, miR-30b, miR-30c, miR-30d and miR-9) is likely to be more potent than that of a single microRNA. The role of mutual repression of BCL6 and PRDM1 in the germinal center to plasma cell differentiation as been described previously. See Martins et al. Immunol. 2008; 26:133-169. Our data suggest that microRNAs may bolster the effects of BCL6 in the inhibition of PRDM1.
Our data show that members of the miR-17˜92 family are consistently expressed in GC cells and may play a role in mature B cell differentiation. Interestingly, the miR-17˜92 family has been implicated in early B cell differentiation and mice lacking the loci that encode these miRNAs have arrested early B cell development. See Ventura et al. Cell. 2008; 132:875-886. The expression patterns of the miR-17˜92 family suggest that the regulatory motifs embedded in the interaction of this miRNA family and its targets might have an additional function in regulating mature B cell differentiation.
A striking observation in this study is the high degree of asymmetry observed in relative expression of miRNAs in GC cells compared to naive and plasma cells. At least 2 hypotheses could account for these findings. First, miRNA expression may promote a highly regulated state that enables GC cells to interact with T cells and antigen presenting cells, and to leave the GC cells poised for differentiation into memory or plasma cells. Second, miRNAs expressed highly in naive and plasma cells may be underrepresented in current miRNA libraries. Such libraries are often constructed from lymph nodes, which are typically enriched in GC cells. High throughput sequencing of sorted populations of B cells could reveal novel miRNAs that are highly expressed in those populations. Interestingly, a larger number of miRNAs were highly expressed in memory cells compared to GC cells. This observation might stem from the fact that memory cells are known to be heterogeneous (Sanz et al. Semin Immunol. 2008; 20:67-82) and standard methods used to select memory cells may capture a diverse group of memory subpopulations.
Example 5 MiRNAs and B Cell Malignancies To examine the expression of B cell stage-specific microRNAs in B cell malignancies, we undertook miRNA profiling of 75 tissue samples derived from normal lymph nodes (N=5) as well as patients with B cell malignancies including the molecular subsets of diffuse large B cell lymphoma (DLBCL; see Rosenwald et al. N Engl J Med. 2002; 346:1937-1947), germinal center B cell-like (GCB) DLBCL (N=20) and activated B cell-like (ABC) DLBCL (N=20), as well as cases of IgV mutated and unmutated chronic lymphocytic leukemia (N=20) and Burkitt lymphoma (N=10).
We constructed a Bayesian predictor from miRNAs that distinguished normal memory B cells from GC cells. See FIG. 1H. We tested the predictor in the B cell malignancies derived from germinal center B cells (Burkitt lymphoma and GCB DLBCL) along with chronic lymphocytic leukemia, which is thought to arise from memory B cells. See Klein et al. J Exp Med. 2001; 194:1625-1638. Using leave one out cross-validation, we found that the predictor constructed from miRNAs expressed in normal B cells was able to correctly identify the B cell specific stage of the B cell malignancy type in over 95% of the cases. See FIG. 4A.
An interesting aspect regarding the role of miRNAs in malignancies is their reported down-regulation in a number of malignancies compared to normal cells from the same lineage. See Lu et al. Nature. 2005; 435:834-838. To further examine this effect in B cell malignancies, we examined the expression of 113 miRNAs that we had identified in normal B cells. See Table 4, above. Of the 113 miRNAs, 103 were detected using the microarrays that we used to profile B cell malignancies and normal lymph nodes. We applied a 2-sided student's t-test to evaluate the relative expression of those 103 miRNAs in B cell malignancies (N=60) and normal lymph nodes (N=5). 34 miRNAs were differentially expressed (P<0.05) at higher levels in malignant cells and 9 miRNAs were expressed more highly in normal cells. 60 miRNAs were not differentially expressed. See FIG. 4B.
As additional validation, we examined miRNA cloning frequencies for sequences cloned from normal and malignant B cells. See Landgraf et al. Cell. 2007; 129:1401-1414. MiRNAs for which a sequence was identified in at least 2 of the 3 available normal B cell samples were used in the analysis. We applied a 2-sided student's t-test (P<0.05) to compare the differential cloning frequency of the miRNAs between normal B cells (N=3) and a variety of mature B cell malignancy patient samples and cell lines (N=42). In all, we found 56 miRNAs that were consistently expressed in normal B cells. We found 13 of those 56 miRNAs were differentially expressed (P<0.05) between normal and malignant B cells, of which 12 miRNAs were expressed more highly in malignant cells and 1 miRNA was expressed more highly in normal cells. See FIG. 4C. In order to avoid effects from tumor-infiltrating non-malignant cells, we repeated the analysis with 20 chronic lymphocytic leukemia samples in the malignant group. The results were similar to those obtained with the larger set of malignancies (data not shown).
These results demonstrate that miRNAs are not down-regulated in B cell malignancies compared to normal B cells and that normal B cell stage-specific miRNAs are maintained in B cell malignancies.
MicroRNA profiling also revealed that each B cell malignancy type had a distinctive pattern of miRNA expression. See FIG. 4D. In order to evaluate the ability of miRNA profiles to distinguish different B cell malignancy types, we constructed Bayesian predictors from the most highly differentially expressed miRNAs for each pair-wise comparison. See Tables 7, 8, and 9.
TABLE 7
Predictor microRNAs that distinguish germinal center B-cell (GCB)
DLBCL from Burkitt Lymphoma, activated B-cell (ABC) DLBCL, and
chronic lymphocytic leukemia
GCBvsBL GCBvsABC GCBvsCLL
hsa-miR-146a hsa-miR-142-3p hsa-miR-126
hsa-miR-154 hsa-miR-16 hsa-miR-130a
hsa-miR-155 hsa-miR-184 hsa-miR-I0b
hsa-miR-184 hsa-miR-191 hsa-miR-154
hsa-miR-29b hsa-miR-19a hsa-miR-193b
hsa-miR-29c hsa-miR-19b hsa-miR-199a-3p
hsa-miR-363 hsa-miR-299-5p hsa-miR-365
hsa-miR-503 hsa-miR-32 hsa-miR-99b
hsa-miR-519c-5p hsa-miR-30e* hsa-miR-143
hsa-miR-301a hsa-miR-151-5p hsa-miR-585
hsa-miR-152 hsa-miR-583 hsa-miR-193a-5p
hsa-miR-30b* mghv-miR-MI-7-5p hsa-miR-886-5p
hsa-miR-590-5p hsa-miR-142-5p hsa-miR-I00
hsa-miR-149* hsa-miR-106b hsa-miR-768-5p
hsa-miR-300 hsa-miR-30e hsa-miR-145
hsa-miR-625* hsa-miR-140-3p hsa-miR-943
kshv-miR-K12-3 hsa-miR-20a hsa-miR-371-5p
hsa-miR-28-5p hsa-miR-526b* hsa-miR-675
hsa-miR-25* hsa-miR-28-5p hsa-miR-150
ebv-miR-BHRFI-2 hsa-miR-30c hsa-miR-181a
BL = Burkitt lymphoma,
ABC = activated B-cell DLBCL,
CLL = chronic lymphocytic lymphoma
TABLE 8
Predictor microRNAs that distinguish activated B-cell (ABC) DLBCL
from Burkitt Lymphoma and chronic lymphocytic leukemia
ABCvsBL ABCvsCLL
hsa-miR-182 hsa-miR-193b
hsa-miR-377 hsa-miR-99b
hsa-miR-660 hsa-miR-551a
hsa-miR-595 mghv-miR-MI-7-3p
ebv-miR-BARTI0 hsa-miR-585
hsa-miR-532-5p hsa-miR-617
hsa-miR-200c* hsa-miR-629*
hsa-miR-362-3p hsa-miR-575
hsa-miR-455-3p kshv-miR-K12-6-5p
hsa-miR-128 hsa-miR-193a-5p
hsa-miR-21* hsa-miR-30e
hsa-miR-589 hsa-miR-296-3p
hsa-miR-135a* hsa-miR-518b
hsa-miR-532-3p hsa-miR-492
hsa-miR-548d-5p hsa-miR-220c
hsa-miR-652 hsa-miR-326
hsa-miR-150* hsa-miR-671-5p
hsa-miR-330-5p hsa-miR-340*
hsa-miR-339-3p hsa-miR-150
hsa-miR-502-3p hsa-miR-193b*
BL = Burkitt lymphoma,
CLL = chronic lymphocytic lymphoma.
TABLE 9
Predictor microRNAs that distinguish Burkitt lymphoma from chronic
lymphocytic leukemia
BLvsCLL
hsa-miR-130b
hsa-miR-154
hsa-miR-155
hsa-miR-29b
hsa-miR-29c
hsa-miR-637
hsa-miR-658
hsa-miR-193a-5p
hsa-miR-886-5p
hsa-miR-768-5p
hsa-miR-101
hsa-miR-933
hsa-miR-371-5p
hsa-miR-675
hsa-miR-150
hsa-miR-874
hsa-miR-181a
hsa-miR-30c
ebv-miR-BHRFI-2
hsa-miR-628-3p
CLL = chronic lymphocytic lymphoma
We tested the performance of the predictor using leave-one-out cross-validation applied to the predictors for Burkitt lymphoma, chronic lymphocytic leukemia, activated B-cell diffuse large B-cell lymphoma, and germinal center B-cell DLBCL. For a sample prediction to be correct, it had to be classified correctly in each pair-wise comparison with all remaining entities. We found it to be over 90% accurate in the identification of each entity. See FIG. 10.
We next expanded the analysis to include follicular lymphoma and Hodgkin's lymphoma. We included the samples from the earlier study and we undertook miRNA profiling of the following additional samples: normal lymph nodes (N=5) and patients with germinal center B cell-like (GCB) DLBCL (N=10), activated B cell-like (ABC) DLBCL (N=10), chronic lymphocytic leukemia (N=10), Burkitt lymphoma (N=10), follicular lymphoma (N=10), and Hodgkin's lymphoma (N=9). As before, microRNA profiling revealed that each B cell malignancy type had a distinctive pattern of miRNA expression. See FIG. 11.
From that data, we identified microRNAs that can be used to distinguish each of the six B cell malignancies analyzed from the other five. Lists of those microRNAs, and whether they are expressed in high levels or low levels relative to the other 5 B cell malignancies, are shown in Tables 10 through 13. In certain embodiments, each of those microRNAs could be used to distinguish one of the six B cell malignancies from the other five. High and low in the table refer to at least a 2 fold difference when compared to other lymphomas and benign lymph nodes.
TABLE 10
Predictor microRNAs that distinguish Burkitt lymphoma, germinal center B-cell
(GCB) DLBCL, and follicular lymphoma from all other B cell malignancies in the study
BL vs GCB vs FL vs
BL miRNA list others GCB miRNA list others FL miRNA list others
hsa-miR-106a High hsa-miR-93/mmu-miR- High hsa-miR-96/mmu-miR- High
hsa-miR-17/mmu- High 93/rno-miR-93 96/rno-miR-96
miR-17/rno-miR-17- hsa-miR-886-3p High hsa-miR-138/mmu-miR- High
5p/rno-miR-17 hsa-miR-365/mmu-miR- High 138/rno-miR-138
hsa-miR-628-3p High 365/rno-miR-365 hsa-miR-342-5p/mmu- High
hsa-miR-193a-5p High hsa-miR-378/mmu-miR- High miR-342-5p/rno-miR-342-
hsa-miR-371-5p High 378/rno-miR-378 5p
hsa-miR-20a/mmu- High hsa-miR-320/mmu-miR- High hsa-miR-337-3p High
miR-20a/rno-miR-20a 320/rno-miR-320 hsa-miR-301a/mmu-miR- High
hsa-miR-23a/mmu- Low hsa-miR-103/mmu-miR- High 301a/rno-miR-301a
miR-23a/rno-miR-23a 103/rno-miR-103 hsa-miR-922 High
hsa-miR-27a/mmu- Low hsa-miR-637 High hsa-miR-744/mmu-miR- High
miR-27a/rno-miR-27a hsa-miR-148a/mmu- High 744
hsa-miR-34b/mmu- Low miR-148a hsa-miR-221* High
miR-34b-3p hsa-miR-199a-3p/hsa- High hsa-miR-215 High
hsa-miR-21/mmu- Low miR-199b-3p/mmu-miR- hsa-miR-197/mmu-miR- High
miR-21/rno-miR-21 199a-3p/mmu-miR- 197
hsa-miR-29a/mmu- Low 199b/rno-miR-199a-3p hsa-miR-92b/mmu-miR- High
miR-29a/rno-miR-29a hsa-miR-126*/mmu- High 92b/rno-miR-92b
hsa-let-7b/mmu-let- Low miR-126-5p/rno-miR- hsa-miR-218-2*/mmu- High
7b/rno-let-7b 126* miR-218-2*/rno-miR-
hsa-let-7f/mmu-let- Low kshv-miR-K12-6-3p High 218*
7f/rno-let-7f hsa-miR-125a-5p/mmu- High hsa-miR-636 High
hsa-miR-29b/mmu- Low miR-125a-5p/rno-miR- hsa-miR-600 High
miR-29b/rno-miR- 125a-5p kshv-miR-K12-7 High
29b hsa-miR-551b/mmu- Low hsa-miR-194/mmu-miR- High
hsa-miR-549 Low miR-551b/rno-miR-551b 194/rno-miR-194
hsa-miR-374a Low hsa-miR-923 Low hsa-miR-524-5p High
hsa-miR-513a-5p Low hsa-miR-22*/mmu-miR- High
hsa-miR-148a/mmu- Low 22*/rno-miR-22*
miR-148a hsa-miR-34c-5p/mmu- High
hsa-miR-223/mmu- Low miR-34c/rno-miR-34c
miR-223/rno-miR-223 hsa-miR-151-3p High
hsa-miR-138- Low hsa-miR-425/mmu-miR- High
1*/mmu-miR- 425/rno-miR-425
138*/rno-miR-138* ebv-miR-BART16 High
hsa-miR-342- Low hsa-miR-20b* High
3p/mmu-miR-342- hsa-miR-574-3p/mmu- High
3p/rno-miR-342-3p miR-574-3p
hsa-miR-146b- Low hsa-miR-155* High
5p/mmu-miR- hsa-miR-374b* High
146b/rno-miR-146b hsa-miR-497/mmu-miR- High
hsa-miR-195/mmu- Low 497/rno-miR-497
miR-195/rno-miR-195 hsa-miR-708/mmu-miR- High
hsa-miR-103/mmu- Low 708/rno-miR-708
miR-103/rno-miR-103 hsa-miR-92a/mmu-miR- High
hsa-miR-888* Low 92a/rno-miR-92a
hsa-miR-363/mmu- Low hsa-miR-361-3p High
miR-363/rno-miR-363 hsa-miR-513a-3p High
hsa-miR-140- Low hsa-miR-934 High
3p/mmu-miR- hsa-miR-363*/rno-miR- High
140*/rno-miR-140* 363*
hsa-miR-191/mmu- Low mghv-miR-M1-3 High
miR-191/rno-miR-191 hsa-miR-129* High
hsa-miR-768-5p Low hsa-miR-148b/mmu-miR- High
hsa-miR-222/mmu- Low 148b/rno-miR-148b-3p
miR-222/rno-miR-222 hsa-miR-493 High
hsa-miR-668/mmu- Low hsa-miR-151-5p/mmu- High
miR-668 miR-151-5p/rno-miR-151
hsa-miR-221/mmu- Low hsa-miR-146b-3p High
miR-221/rno-miR-221 hsa-miR-886-5p High
hsa-miR-24-1*/mmu- Low hsa-miR-331-3p/mmu- High
miR-24-1*/rno-miR- miR-331-3p/rno-miR-331
24-1* hsa-miR-574-5p/mmu- High
hsa-miR-29c/mmu- Low miR-574-5p
miR-29c/rno-miR-29c hsa-miR-424 High
hsa-miR-146a/mmu- Low hsa-miR-152/mmu-miR- High
miR-146a/rno-miR- 152/rno-miR-152
146a hsa-miR-302a/mmu-miR- High
hsa-miR-154/mmu- Low 302a
miR-154/rno-miR-154 hsa-miR-181a/mmu-miR- High
hsa-miR-215 Low 181a/rno-miR-181a
hsa-miR-487b/mmu- Low hsa-miR-509-5p High
miR-487b/rno-miR- hsa-let-7d/mmu-let- High
487b 7d/rno-let-7d
hsa-miR-155 Low hsa-miR-18b High
hsa-miR-620 High
hsa-miR-18a/mmu-miR- High
18a/rno-miR-18a
hsa-miR-298 High
hsa-miR-98/mmu-miR- High
98/rno-miR-98
hsa-miR-576-3p High
hsa-miR-374b/mmu-miR- High
374/rno-miR-374
hsa-miR-32* High
hsa-miR-302d* High
ebv-miR-BART17-5p High
hsa-miR-620 High
hsa-miR-665 High
hsa-miR-185/mmu-miR- High
185/rno-miR-185
hsa-miR-34b/mmu-miR- High
34b-3p
hsa-let-7e/mmu-let- High
7e/rno-let-7e
TABLE 11
Predictor microRNAs that distinguish activated B-cell (ABC) DLBCL
from all other B cell malignancies in the study
ABC vs
ABC miRNA list others
hsa-miR-126/mmu-miR- High
126-3p/rno-miR-126
hsa-miR-125b/mmu-miR- High
125b-5p/rno-miR-125b-5p
hsa-miR-145/mmu-miR- High
145/rno-miR-145
hsa-miR-22/mmu-miR- High
22/rno-miR-22
hsa-miR-21/mmu-miR- High
21/rno-miR-21
hsa-miR-199a-3p/hsa-miR- High
199b-3p/mmu-miR-199a-
3p/mmu-miR-199b/rno-
miR-199a-3p
hsa-miR-24/mmu-miR- High
24/rno-miR-24
hsa-miR-143/mmu-miR- High
143/rno-miR-143
hsa-miR-23b/mmu-miR- High
23b/rno-miR-23b
hsa-miR-23a/mmu-miR- High
23a/rno-miR-23a
hsa-miR-142-3p/mmu-miR- High
142-3p/rno-miR-142-3p
hsa-let-7a/mmu-let-7a/rno- High
let-7a
hsa-miR-146b-5p/mmu- High
miR-146b/rno-miR-146b
hsa-miR-27a/mmu-miR- High
27a/rno-miR-27a
hsa-miR-30a/mmu-miR- High
30a/rno-miR-30a
hsa-miR-27b/mmu-miR- High
27b/rno-miR-27b
hsa-let-7c/mmu-let-7c/rno- High
let-7c
hsa-miR-921 Low
ebv-miR-BHRF1-2 Low
hsa-miR-199a-5p/mmu- Low
miR-199a-5p/rno-miR-
199a-5p
hsa-miR-768-5p Low
hsa-miR-491-3p Low
hsa-miR-185/mmu-miR- Low
185/rno-miR-185
ebv-miR-BART17-5p Low
hsa-miR-32* Low
hsa-miR-335/mmu-miR- Low
335-5p/rno-miR-335
hsa-miR-149* Low
hsa-miR-576-3p Low
hsa-miR-214/mmu-miR- Low
214/rno-miR-214
hsa-miR-184/mmu-miR- Low
184/rno-miR-184
hsa-miR-520d-5p Low
hsa-miR-518c* Low
hsa-miR-801/mmu-miR-801 Low
hsa-miR-298 Low
hsa-miR-634 Low
hsa-miR-583 Low
hsa-miR-187* Low
hsa-miR-30d/mmu-miR- Low
30d/rno-miR-30d
hsa-miR-129-5p/mmu-miR- Low
129-5p/rno-miR-129
hsa-miR-300 Low
hsa-miR-620 Low
hsa-miR- Low
130b*/mmu-miR-
130b*
hsa-miR-20b/mmu- Low
miR-20b/rno-miR-
20b-5p
kshv-miR-K12-3 Low
hsa-miR-28-3p/rno- Low
miR-28*
hsa-miR-557 Low
hsa-miR-150/mmu- Low
miR-150/rno-miR-
150
hsa-miR-98/mmu- Low
miR-98/rno-miR-98
hsa-miR-486- Low
hsa-miR-518a-
5p/hsa-miR-527
hsa-miR-302d* Low
hsa-miR-516a-5p Low
hsa-miR-148b/mmu- Low
miR-148b/rno-miR-
148b-3p
hsa-miR-25* Low
hsa-miR-374b/mmu- Low
miR-374/rno-miR-
374
hsa-miR-638 Low
hsa-miR-302a/mmu- Low
miR-302a
hsa-miR-99b*/mmu- Low
miR-99b*/rno-miR-
99b*
hsa-miR-29c*/mmu- Low
miR-29c*/rno-miR-
29c*
hsa-miR-138/mmu- Low
miR-138/rno-miR-
138
hsa-miR-766 Low
hsa-miR-488 Low
hsa-miR-498 Low
hsa-miR-339- Low
5p/mmu-miR-339-
5p/rno-miR-339-5p
hsa-miR-193b* Low
hsa-miR-299- Low
5p/mmu-miR-
299*/rno-miR-299
ebv-miR-BART8* Low
hsa-miR-107/mmu- Low
miR-107/rno-miR-
107
hsa-miR-519e* Low
hsa-miR-146b-3p Low
hsa-miR-552 Low
hsa-miR-509-5p Low
hsa-miR-574- Low
5p/mmu-miR-574-
5p
hsa-miR-524-5p Low
mghv-miR-M1-7-5p Low
hsa-miR-659 Low
hcmv-miR-UL148D Low
hsa-miR-92a/mmu- Low
miR-92a/rno-miR-
92a
hsa-miR- Low
30e*/mmu-miR-
30e*/rno-miR-
30e*
hsa-miR- Low
183*/mmu-miR-
183*
hsa-miR-144* Low
hsa-miR-574- Low
3p/mmu-miR-574-
3p
hsa-miR-889 Low
hsa-miR-525-5p Low
kshv-miR-K12-8 Low
hsa-miR-32/mmu- Low
miR-32/rno-miR-
32
hsa-miR-938 Low
hsa-miR-198 Low
hsa-miR- Low
186/mmu-miR-
186/rno-miR-186
hsa-miR- Low
18a/mmu-miR-
18a/rno-miR-18a
hsa-miR-516b Low
hsa-miR-625* Low
hsa-miR-551b* Low
hsa-miR-885-5p Low
hsa-miR-891a Low
hsa-miR- Low
340/mmu-miR-
340-5p/rno-miR-
340-5p
hsa-let-7d/mmu- Low
let-7d/rno-let-7d
hsa-miR-151- Low
5p/mmu-miR-151-
5p/rno-miR-151
hsa-miR-18b Low
ebv-miR-BHRF1-1 Low
hsa-miR-510 Low
hsa-miR-625 Low
mghv-miR-M1-8 Low
ebv-miR-BART19- Low
3p
hsa-miR-147 Low
hsa-miR-28- Low
5p/mmu-miR-
28/rno-miR-28
ebv-miR-BART13 Low
hsa-miR-25/mmu- Low
miR-25/rno-miR-
25
hsa-miR-519d Low
hsa-miR-361- Low
5p/mmu-miR-
361/rno-miR-361
hsa-miR-331- Low
3p/mmu-miR-331-
3p/rno-miR-331
hsa-miR-423- Low
3p/mmu-miR-423-
3p/rno-miR-423
hsa-miR-93/mmu- Low
miR-93/rno-miR-
93
TABLE 12
Predictor microRNAs that distinguish chronic lymphocytic leukemia
from all other B cell malignancies in the study
CLL vs
CLL miRNA list others
hsa-miR- High
30e*/mmu-miR-
30e*/rno-miR-30e*
hsa-miR-32/mmu- High
miR-32/rno-miR-32
hsa-let-7g/mmu- High
let-7g
hsa-miR-186/mmu- High
miR-186/rno-miR-
186
hsa-miR-140- High
5p/mmu-miR-
140/rno-miR-140
hsa-miR- High
196a*/mmu-miR-
196a*/rno-miR-
196a*
hsa-miR- High
487b/mmu-miR-
487b/rno-miR-487b
hsa-miR-150/mmu- High
miR-150/rno-miR-
150
hsa-miR-147 High
hsa-miR-486- High
5p/mmu-miR-486
hsa-miR-144* High
hsa-miR-154/mmu- High
miR-154/rno-miR-
154
hsa-miR-28- High
5p/mmu-miR-
28/rno-miR-28
hsa-miR-299- High
5p/mmu-miR-
299*/rno-miR-299
hsa-miR-33a/mmu- High
miR-33/rno-miR-33
hsa-miR-363/mmu- High
miR-363/rno-miR-
363
hsa-miR-891a High
hsa-miR-768-5p High
hsa-miR-361- High
5p/mmu-miR-
361/rno-miR-361
hsa-miR-519d High
hsa-miR-335/mmu- High
miR-335-5p/rno-
miR-335
hsa-miR-668/mmu- High
miR-668
hsa-let-7f/mmu-let- High
7f/rno-let-7f
hsa-miR-24- High
1*/mmu-miR-24-
1*/rno-miR-24-1*
hsa-miR-223/mmu- High
miR-223/rno-miR-
223
hsa-miR-140- High
3p/mmu-miR-
140*/rno-miR-140*
hsa-miR-144/mmu- High
miR-144/rno-miR-
144
hsa-miR-638 High
hsa-miR-30d/mmu- High
miR-30d/rno-miR-
30d
hsa-miR-423- High
3p/mmu-miR-423-
3p/rno-miR-423
hsa-miR-155 High
hsa-miR-101/mmu- High
miR-101a/rno-miR-
101a
hsa-miR-20b/mmu- High
miR-20b/rno-miR-
20b-5p
hsa-miR-374a High
hsa-miR-25/mmu- High
miR-25/rno-miR-25
hsa-miR-199a- High
5p/mmu-miR-199a-
5p/rno-miR-199a-
5p
hsa-miR-649 High
hsa-miR-191/mmu- High
miR-191/rno-miR-
191
hsa-miR-30e/mmu- High
miR-30e/rno-miR-
30e
hsa-miR-107/mmu- High
miR-107/rno-miR-
107
hsa-miR-93/mmu- High
miR-93/rno-miR-93
hsa-miR-29c/mmu- High
miR-29c/rno-miR-
29c
hsa-miR-541* High
hsa-miR-888* High
hsa-miR-549 High
hsa-miR-19a/mmu- High
miR-19a/rno-miR-
19a
hsa-miR-342- High
3p/mmu-miR-342-
3p/rno-miR-342-3p
hsa-miR-142- High
5p/mmu-miR-142-
5p/rno-miR-142-5p
hsa-miR-801/mmu- High
miR-801
hsa-let-7i/mmu-let- High
7i/rno-let-7i
hsa-miR-26a/mmu- High
miR-26a/rno-miR-
26a
hsa-miR-15a/mmu- High
miR-15a
hsa-miR-195/mmu- High
miR-195/rno-miR-
195
hsa-miR- High
106b/mmu-miR-
106b/rno-miR-106b
hsa-miR-26b/mmu- High
miR-26b/rno-miR-
26b
hsa-miR-15b/mmu- High
miR-15b/rno-miR-
15b
hsa-miR-222/mmu- High
miR-222/rno-miR-
222
hsa-miR-185/mmu- High
miR-185/rno-miR-
185
hsa-miR-550 High
hsa-let-7e/mmu- Low
let-7e/rno-let-7e
hsa-miR-24/mmu- Low
miR-24/rno-miR-24
hsa-miR-30c- Low
2*/mmu-miR-30c-
2*/rno-miR-30c-2*
hsa-miR-765 Low
mghv-miR-M1-4 Low
hsa-miR-933 Low
hsa-miR-620 Low
hsa-miR-30b* Low
hsa-miR-658 Low
hsa-miR-10a/mmu- Low
miR-10a/rno-miR-
10a-5p
hsa-miR-665 Low
hsa-miR-185* Low
hsa-miR-503 Low
hsa-miR-126*/mmu-miR- Low
126-5p/rno-miR-126*
hsa-miR-10b/mmu-miR- Low
10b/rno-miR-10b
hsa-miR-628-3p Low
hsa-miR-422a Low
hsa-miR-193a-5p Low
hsa-miR-143/mmu-miR- Low
143/rno-miR-143
hsa-miR-371-5p Low
hsa-miR-100/mmu-miR- Low
100/rno-miR-100
hsa-miR-365/mmu-miR- Low
365/rno-miR-365
hsa-miR-145/mmu-miR- Low
145/rno-miR-145
kshv-miR-K12-6-3p Low
ebv-miR-BART6-3p Low
hsa-miR-220c Low
hsa-miR-519c-5p/hsa-miR- Low
519b-5p/hsa-miR-523*/hsa-
miR-518e*/hsa-miR-
522*/hsa-miR-519a*
hsa-miR-130a/mmu-miR- Low
130a/rno-miR-130a
hsa-miR-424 Low
hsa-miR-483-5p Low
hsa-miR-193b Low
hsa-miR-637 Low
hsa-miR-920 Low
hsa-miR-488 Low
ebv-miR-BHRF1-2 Low
hsa-miR-526b Low
hsa-miR-126/mmu-miR-126- Low
3p/rno-miR-126
hsa-miR-943 Low
hsa-miR-199a-3p/hsa-miR- Low
199b-3p/mmu-miR-199a-
3p/mmu-miR-199b/rno-miR-
199a-3p
mghv-miR-M1-3 Low
hsa-miR-934 Low
hsa-miR-886-5p Low
hsa-miR-200b*/mmu-miR- Low
200b*
hsa-miR-485-3p/mmu-miR- Low
485*
hsa-miR-181a/mmu-miR- Low
181a/rno-miR-181a
hsa-miR-125b/mmu-miR- Low
125b-5p/rno-miR-125b-5p
TABLE 13
Predictor microRNAs that distinguish Hodgkin's lympoma from all
other B cell malignancies in the study
HL vs
HL miRNA list others
hsa-miR-338- High
5p/mmu-miR-338-
5p/rno-miR-338*
hsa-miR-433/mmu- High
miR-433/rno-miR-
433
hsa-miR-552 High
hsa-miR-202 High
hsa-miR-299-3p High
hsa-miR-509-3-5p High
hsa-miR-490-5p High
hsa-miR-508-5p High
hsa-miR-181a-2* High
hsa-miR-663 High
hsa-miR-326/mmu- High
miR-326/rno-miR-
326
hsa-miR-542- High
3p/mmu-miR-542-
3p/rno-miR-542-3p
hsa-miR-492 High
hsa-miR-584 High
hsa-miR-654-5p High
ebv-miR-BART20-3p High
hsa-miR-542- High
5p/mmu-miR-542-
5p/rno-miR-542-5p
ebv-miR-BART9* High
hsa-miR-124/mmu- High
miR-124/rno-miR-
124
hsa-miR-551a High
hsa-miR-208a/mmu- High
miR-208a/rno-miR-
208
hsa-miR-220b High
hsa-miR-615- High
3p/mmu-miR-615-3p
hsa-miR- High
135a*/mmu-miR-
135a*
hiv1-miR-H1 High
hsa-miR-124*/mmu- High
miR-124*/rno-miR-
124*
hsa-miR-502-5p High
hsa-miR-92b* High
hsa-miR-518a-3p High
hsa-miR-377* High
hsa-miR-125a- High
3p/mmu-miR-125a-
3p/rno-miR-125a-3p
hsa-miR-30c- High
1*/mmu-miR-30c-
1*/rno-miR-30c-1*
hsa-miR-650 High
hsa-miR-629 High
hsa-miR-296- High
3p/mmu-miR-296-
3p/rno-miR-296
hsa-miR-425*/mmu- High
miR-425*
hsa-miR-514 High
hsa-miR-519e High
hsa-miR-938 High
hsa-miR-340*/mmu- High
miR-340-3p/rno-
miR-340-3p
hsa-miR-657 High
hsa-miR-9*/mmu- High
miR-9*/rno-miR-9*
ebv-miR-BART7* High
hsa-miR-612 High
hsa-miR-640 High
hsa-miR-623 High
hsa-miR-99b*/mmu- High
miR-99b*/rno-miR-
99b*
hsa-miR-645 High
hsa-miR-484/mmu- High
miR-484/rno-miR-
484
hsa-miR-376a* High
hsa-miR-345 High
hsa-miR-586 High
hsa-miR-622 High
hsa-miR-206/mmu- High
miR-206/rno-miR-
206
hcmv-miR-US25-1* High
hsa-miR-302c* High
hsa-miR- High
106b*/mmu-miR-
106b*/rno-miR-
106b*
hsa-miR-500 High
hsa-miR-890 High
hsa-miR-10a*/mmu- High
miR-10a*/rno-miR-
10a-3p
kshv-miR-K12-1 High
hsa-miR-629* High
hsa-miR-193b* High
ebv-miR-BHRF1-3 High
hsa-miR-183/mmu- High
miR-183/rno-miR-
183
hsa-let-7b*/mmu- High
let-7b*/rno-let-7b*
hsa-miR-409- High
5p/mmu-miR-409-
5p/rno-miR-409-5p
hsa-miR-585 High
hsa-miR-526b* High
hsa-miR-337-3p High
hsa-miR-212/mmu- High
miR-212/rno-miR-
212
hsa-miR-548b-3p High
hcmv-miR-UL112 High
hsa-miR-601 High
hsa-let-7d*/mmu- High
let-7d*/rno-let-7d*
hsa-miR-181b/mmu- High
miR-181b/rno-miR-
181b
hsa-miR-195* High
kshv-miR-K12-5 High
hsa-miR-500* High
hsa-miR-24- High
2*/mmu-miR-24-
2*/rno-miR-24-2*
hsa-miR-382/mmu- High
miR-382/rno-miR-
382
ebv-miR-BART8* High
hsa-miR-125b- High
2*/rno-miR-125b*
hsa-miR-194* High
hsa-miR-297/mmu- High
miR-297a
hsa-miR-610 High
hsa-miR-575 High
hsa-miR-21* High
hsa-miR-936 High
kshv-miR-K12-6-5p High
hsa-miR-553 High
hsa-miR-652/mmu- High
miR-652/rno-miR-
652
hsa-miR-877/mmu- High
miR-877/rno-miR-
877
hsa-miR-526a/hsa- High
miR-520c-5p/hsa-
miR-518d-5p
hsa-miR-122* High
hsa-miR-576-5p High
mghv-miR-M1-6 High
hsa-miR-551b* High
hsa-miR-125b- High
1*/mmu-miR-125b-
3p/rno-miR-125b-
3p
hsa-miR-137/mmu- High
miR-137/rno-miR-
137
ebv-miR-BART18-3p High
hsa-miR-452 High
hsa-miR-23a*/rno- High
miR-23a*
hsa-miR-617 High
hsa-miR-550* High
hsa-miR-557 High
hsa-miR-331- High
5p/mmu-miR-331-
5p
hsa-miR-296- High
5p/mmu-miR-296-
5p/rno-miR-296*
mghv-miR-M1-2 High
ebv-miR-BART6-3p High
hsa-miR-518b High
hsa-miR-99b/mmu- High
miR-99b/rno-miR-
99b
hsa-miR-525-5p High
hsa-miR-589 High
hsa-miR-7-2* High
hsa-miR-490- High
3p/mmu-miR-490
hsa-miR- High
150*/mmu-miR-
150*
hsa-miR-17*/rno- High
miR-17-3p
hsa-miR-509-3p High
ebv-miR-BHRF1-1 High
hsa-miR- High
183*/mmu-miR-
183*
hsa-miR-635 High
hsa-miR- High
130b/mmu-miR-
130b/rno-miR-130b
mghv-miR-M1-8 High
hsa-miR-887 High
hsa-miR-210/mmu- High
miR-210/rno-miR-
210
hsa-miR-766 High
hsa-miR-671- High
5p/mmu-miR-671-
5p
hsa-miR-659 High
hsa-miR-330- High
5p/mmu-miR-
330/rno-miR-330
hsa-miR-323- High
3p/mmu-miR-323-
3p/rno-miR-323
ebv-miR-BART13 High
ebv-miR-BART5 High
hsa-miR-602 High
hcmv-miR-UL148D High
hsa-miR-373* High
hsa-miR-526b High
hsa-miR-328/mmu- High
miR-328/rno-miR-
328
hsa-miR-874/mmu- High
miR-874/rno-miR-
874
ebv-miR-BART19-3p High
hsa-miR-595 High
hsa-miR-889 High
mghv-miR-M1-7-5p High
hsa-miR-483-5p High
hsa-miR- High
487b/mmu-miR-
487b/rno-miR-487b
hsa-miR-675 High
hsa-miR-220c High
mghv-miR-M1-7-3p High
hsa-miR-485- High
3p/mmu-miR-485*
hsa-miR-198 High
hsa-miR-483-3p High
hcmv-miR-UL70-3p High
hsa-miR-149/mmu- High
miR-149
hsa-miR-516a-5p High
hsa-miR- High
145*/mmu-miR-
145*
hsa-miR-656 High
hsa-miR-502-3p High
hsa-miR-29c*/mmu-miR- High
29c*/rno-miR-29c*
hsa-miR-937 High
hsa-miR-515-5p High
hsa-miR-153/mmu-miR- High
153/rno-miR-153
hsa-miR-519e* High
hsa-miR-128/mmu-miR- High
128/rno-miR-128
hsa-miR-516b High
hsa-miR-532-5p/mmu-miR- High
532-5p/rno-miR-532-5p
kshv-miR-K12-8 High
hsa-miR-455-3p High
hsa-miR-27a*/mmu-miR- High
27a*/rno-miR-27a*
hsa-miR-510 High
hsa-miR-505/rno-miR-505 High
hsa-miR-187* High
hsa-miR-498 High
hsa-miR-625 High
hsa-miR-129-5p/mmu-miR- High
129-5p/rno-miR-129
ebv-miR-BHRF1-2 High
hsa-miR-143* High
kshv-miR-K12-3 High
hsa-miR-660 High
hsa-miR-25* High
hsa-miR-29a*/mmu-miR- High
29a*/rno-miR-29a*
hsa-miR-422a High
hsa-miR-518a-5p/hsa-miR- High
527
hsa-miR-519c-5p/hsa-miR- High
519b-5p/hsa-miR-
523*/hsa-miR-518e*/hsa-
miR-522*/hsa-miR-519a*
hsa-miR-28-3p/rno-miR-28* High
hsa-miR-300 High
hsa-miR-130a/mmu-miR- High
130a/rno-miR-130a
hsa-miR-583 High
hsa-miR-149* High
hsa-miR-184/mmu-miR- High
184/rno-miR-184
hsa-miR-625* High
hsa-miR-99a/mmu-miR- High
99a/rno-miR-99a
hsa-miR-199b-5p High
hsa-miR-513a-5p High
hsa-miR-494/mmu-miR- High
494/rno-miR-494
mghv-miR-M1-4 High
hsa-miR-634 High
hsa-miR-923 High
hsa-miR-503 High
ebv-miR-BART2-3p High
hsa-miR-520d-5p High
hsa-miR-30b* High
hsa-miR-30c-2*/mmu-miR- High
30c-2*/rno-miR-30c-2*
hsa-miR-658 High
hsa-miR-921 High
hsa-miR-423-5p/mmu-miR- High
423-5p
hsa-miR-933 High
hsa-miR-23b/mmu-miR- Low
23b/rno-miR-23b
hsa-miR-27b/mmu-miR- Low
27b/rno-miR-27b
hsa-miR-550 Low
hsa-let-7a/mmu-let-7a/rno- Low
let-7a
hsa-miR-24/mmu-miR- Low
24/rno-miR-24
hsa-miR-451/mmu-miR- Low
451/rno-miR-451
hsa-miR-30a/mmu-miR- Low
30a/rno-miR-30a
hsa-miR-20b/mmu-miR- Low
20b/rno-miR-20b-5p
hsa-miR-26a/mmu-miR- Low
26a/rno-miR-26a
hsa-miR-26b/mmu-miR- Low
26b/rno-miR-26b
hsa-miR-101/mmu-miR- Low
101a/rno-miR-101a
hsa-miR-106b/mmu-miR- Low
106b/rno-miR-106b
hsa-miR-16/mmu-miR- Low
16/rno-miR-16
hsa-miR-29b/mmu-miR- Low
29b/rno-miR-29b
hsa-miR-768-3p Low
hsa-miR-30e/mmu-miR- Low
30e/rno-miR-30e
hsa-miR-106a Low
hsa-miR-142-5p/mmu-miR- Low
142-5p/rno-miR-142-5p
hsa-miR-144/mmu-miR- Low
144/rno-miR-144
hsa-miR-17/mmu-miR- Low
17/rno-miR-17-5p/rno-miR-
17
hsa-miR-15b/mmu-miR- Low
15b/rno-miR-15b
hsa-miR-30c/mmu-miR- Low
30c/rno-miR-30c
hsa-miR-142-3p/mmu-miR- Low
142-3p/rno-miR-142-3p
hsa-miR-20a/mmu-miR- Low
20a/rno-miR-20a
hsa-miR-30b/mmu-miR- Low
30b/rno-miR-30b-5p
hsa-miR-19a/mmu-miR- Low
19a/rno-miR-19a
hsa-miR-19b/mmu-miR- Low
19b/rno-miR-19b
From the data in Tables 10 through 13, we identified subsets of microRNAs that are sufficient to distinguish each of the six B cell malignancies from the other five. We selected only microRNAs that are expressed more highly in the selected B cell malignancy. Those microRNAs are listed in Tables 14 and 15. In certain embodiments, each of the microRNAs listed in Tables 14 and 15 can be used to distinguish one B cell malignancy from the other five.
TABLE 14
Predictor microRNAs that distinguish activated B-cell (ABC) DLBCL, germinal
center B-cell like (GCB) DLBCL, and Burkitt lymphoma
ABC High GCB High BL High
hsa-miR-22/mmu-miR-22/rno-miR- hsa-miR-93/mmu-miR-93/rno-miR-93 hsa-miR-628-
22 hsa-miR-103/mmu-miR-103/rno-miR-103 3p
hsa-miR-21/mmu-miR-21/rno-miR- hsa-miR-320/mmu-miR-320/rno-miR-320
21 hsa-miR-125a-5p/mmu-miR-125a-5p/rno-miR-
hsa-miR-24/mmu-miR-24/rno-miR- 125a-5p
24
hsa-miR-23b/mmu-miR-23b/rno-
miR-23b
hsa-miR-23a/mmu-miR-23a/rno-
miR-23a
hsa-let-7a/mmu-let-7a/rno-let-7a
hsa-let-7c/mmu-let-7c/rno-let-7c
TABLE 15
Predictor microRNAs that distinguish chromic lymphocytic leukemia, follicular
lymphoma, and Hodgkin's lymphoma
CLL High FL High HL High
hsa-miR-32/mmu-miR-32/rno- hsa-miR-152/mmu-miR- hsa-miR-498
miR-32 152/rno-miR-152 hsa-miR-525-5p
hsa-miR-150/mmu-miR-150/rno- hsa-miR-885-5p hsa-miR-551b*
miR-150 hsv1-miR-H1 hsa-miR-340*/mmu-miR-340-
hsa-miR-140-5p/mmu-miR- hsa-miR-548d-5p 3p/rno-miR-340-3p
140/rno-miR-140 hsa-miR-488 hsa-miR-494/mmu-miR-494/rno-
hsa-let-7g/mmu-let-7g ebv-miR-BART16 miR-494
hsa-miR-154/mmu-miR-154/rno- hsa-miR-22*/mmu-miR- hsa-miR-183*/mmu-miR-183*
miR-154 22*/rno-miR-22* hsa-miR-659
hsa-miR-486-5p/mmu-miR-486 hsa-miR-513a-3p hsa-miR-193b*
hsa-miR-101/mmu-miR- hsa-miR-708/mmu-miR- hsa-miR-766
101a/rno-miR-101a 708/rno-miR-708 hsa-miR-516a-5p
hsa-miR-30e/mmu-miR-30e/rno- hsa-miR-425/mmu-miR- hsa-miR-125b-1*/mmu-miR-
miR-30e 425/rno-miR-425 125b-3p/rno-miR-125b-3p
hsa-miR-768-5p hsa-miR-337-3p ebv-miR-BART6-3p
hsa-miR-363/mmu-miR-363/rno- ebv-miR-BART17-5p ebv-miR-BART8*
miR-363 hsa-miR-221* hsa-miR-509-3-5p
hsa-miR-668/mmu-miR-668 hsa-miR-92b/mmu-miR- hsa-miR-602
hsa-miR-147 92b/rno-miR-92b ebv-miR-BHRF1-1
hsa-miR-196a*/mmu-miR- hsa-miR-197/mmu-miR-197 mghv-miR-M1-2
196a*/rno-miR-196a* hsa-miR-32* hsa-miR-145*/mmu-miR-145*
hsa-miR-142-5p/mmu-miR-142- hsa-miR-342-5p/mmu-miR- hsa-miR-296-5p/mmu-miR-296-
5p/rno-miR-142-5p 342-5p/rno-miR-342-5p 5p/rno-miR-296*
hsa-miR-199a-5p/mmu-miR- hsa-miR-524-5p hsa-miR-17*/rno-miR-17-3p
199a-5p/rno-miR-199a-5p hsa-miR-34c-5p/mmu-miR- hsa-miR-452
hsa-miR-24-1*/mmu-miR-24- 34c/rno-miR-34c hsa-miR-326/mmu-miR-326/rno-
1*/rno-miR-24-1* hsa-let-7e/mmu-let-7e/rno-let- miR-326
hsa-miR-891a 7e hsa-miR-652/mmu-miR-652/rno-
hsa-miR-550 hsa-miR-151-3p miR-652
hsa-miR-801/mmu-miR-801 hsa-miR-744/mmu-miR-744 hsa-miR-623
hsa-miR-549 hsa-miR-574-3p/mmu-miR- hsa-miR-194*
hsa-miR-888* 574-3p hsa-miR-125a-3p/mmu-miR-
hsa-miR-600 125a-3p/rno-miR-125a-3p
hsa-miR-20b* hsa-miR-10a*/mmu-miR-
hsa-miR-194/mmu-miR- 10a*/rno-miR-10a-3p
194/rno-miR-194 hsa-miR-519e
hsa-miR-363*/rno-miR-363* hsa-miR-502-5p
hsa-miR-155* hsa-miR-124*/mmu-miR-
hsa-miR-34b/mmu-miR-34b-3p 124*/rno-miR-124*
hsa-miR-922 hsa-miR-345
hsa-miR-497/mmu-miR- hsa-miR-584
497/rno-miR-497 hsa-miR-650
hsa-miR-493 hsa-miR-202
hsa-miR-138/mmu-miR- hsa-miR-548b-3p
138/rno-miR-138 hsa-miR-492
hsa-miR-215 hsa-miR-135a*/mmu-miR-135a*
hsa-miR-302a/mmu-miR-302a ebv-miR-BART20-3p
hsa-miR-96/mmu-miR-96/rno- hsa-miR-586
miR-96 hsa-miR-338-5p/mmu-miR-338-
hsa-miR-218-2*/mmu-miR- 5p/rno-miR-338*
218-2*/rno-miR-218* hsa-miR-92b*
kshv-miR-K12-7 hiv1-miR-H1
hsa-miR-301a/mmu-miR- hsa-miR-508-5p
301a/rno-miR-301a hsa-miR-542-5p/mmu-miR-542-
5p/rno-miR-542-5p
hsa-miR-490-5p
hsa-miR-663
hsa-miR-433/mmu-miR-433/rno-
miR-433
Finally, we identified sets of microRNAs that can distinguish between each pair of B cell malignancies in the study. Lists of those microRNAs are shown in Appendix B, Tables 16 to 30. In certain embodiments, each of the listed microRNAs is sufficient to distinguish between the two B cell malignancies listed in the table description.
The consistent expression of a number of miRNAs in a diverse set of B cell malignances suggests a role for miRNAs in the maintenance of tumor phenotype. Assays for stage-specific B cell markers such as BCL6, a marker for GC cells, are essential in the clinical diagnosis of B cell malignancies. Our data suggest that stage-specific biology in B cell malignancies is retained at the miRNA level. Recent work has demonstrated the utility of gene expression profiling in reliably distinguishing closely related B cell malignancies. See Hummel et al. N Engl J Med. 2006; 354:2419-2430; Dave et al. N Engl J Med. 2006; 354:2431-2442. However, clinical translation of gene expression profiling has proved to be difficult because of the need for freshly frozen tissue. Since intact miRNAs can be isolated from tissues preserved using standard methods (see, e.g., Doleshal et al. J Mol Diagn. 2008; 10:203-211; Xi et al. Rna. 2007; 13:1668-1674), diagnostic methods based upon miRNA profiles could be fairly easy to translate to clinical use.
Interestingly, in contrast to a previous study (Lu et al. Nature 2005; 435:834-838), we did not note a decrease in the expression of the total number or overall expression levels of miRNAs in B cell malignancies compared to normal lymph nodes. Although B cell malignancies maintain the expression of a number of stage-specific miRNAs, their miRNA expression patterns are clearly deranged compared to normal lymph nodes. The consequences of altered miRNA expression in B cell tumors would be important to explore in future studies.
In conclusion, our study demonstrates that mature B cell subsets have distinct patterns of microRNA expression, suggesting a role for miRNAs in B cell differentiation. We provide experimental evidence that transcription factors such as LM02 and PRDM1 are direct targets of differentially expressed miRNAs. B cell malignancies demonstrate a distinct pattern of miRNA expression that could be useful in distinguishing morphologically identical subtypes of these tumors. The conserved expression of stage-specific microRNAs in normal and malignant B cells suggests a role for microRNAs in the maintenance of the mature B cell phenotype.
Example 6 Differential Expression of miRNAs in ABC DLBCL Vs. GBC DLBCL Malignancies As discussed above, microRNAs have shown promise as biomarkers in a number of malignancies. Diffuse large B cell lymphoma (DLBCL) is the most common form of lymphoma and is known to comprise at least 2 molecularly distinct subgroups with different responses to standard therapy. These two distinct subgroups are typically identified as ABC DLBCL and GC (or GCB) DLBCL.
However, the current methods used to distinguish GCB from non GCB DLBCL are limited fashion and can yield inconsistent results. While gene expression profiling remains the gold standard for distinguishing these 2 molecular subgroups it is not routinely performed in clinical laboratories. In an effort to expand and improve the existing analytical options we sought to develop microRNA-based assays. We created RNA libraries from 31 different samples and performed deep sequencing analysis to identify the present miRNAs.
Small RNA Library Creation and Deep Sequencing
Total RNA was extracted from the 31 samples using the phenol-chloroform method to preserve microRNAs, using Ambion reagents. Total RNA (typically 5 μg) from each sample was run on denaturing polyacrylamide-urea gels. The ˜17-25 nucleotide RNAs were excised from the gel, ligated to sequencing adaptors on both ends and reverse transcribed. The resulting cDNA library was PCR-amplified for 15 cycles and gel purified on 6% acrylamide gel. The gel-purified amplicon quality and quantity were analyzed on a 6% acrylamide gel relative to oligonucleotides of known concentration and size. 120 μl of 1-4 μM library were loaded on to the Illumina cluster station, where DNA molecules were attached to high-density universal adaptors in the flow cells and amplified. The DNA clusters generated via this process were sequenced with sequencing-by-synthesis technology, where successive high-resolution images of the four-color fluorescence excitation dependent on the base incorporated during each cycle were captured. Sequencing reads were generated for each of the 31 samples and base calls were rendered using Illumina software. All the primary sequencing data and gene expression data is publicly available through the GEO archive through accession GSE22898.
The small RNA libraries from the 31 samples which were subjected to massively parallel, high-throughput sequencing using the Illumina platform generated a total of 328 million separate reads. Our approach to analyzing the sequences and discovering microRNAs broadly follows a previously described method (see, Zheng, Q., et al. GOEAST: a web-based software toolkit for Gene Ontology enrichment analysis, Nucleic acids Res. 2008; 36 (Web Server issue: W358-363). All bioinformatics analyses were performed using a cluster of 1024 Linux computer nodes. Preprocessing was carried out using locally written Shell and Perl scripts.
From the raw sequences generated by high-throughput sequencing, the 3′- and 5′-adaptor sequences were trimmed. Low quality sequences were identified as those sequencing reads that contained stretches of consecutive identical bases or uncalled nucleotides (N) in the first 12 bases and sequencing reads shorter than 17 nucleotides. To minimize redundancy, reads were initially curtailed to the first 22 nucleotides and identical sequences were represented with a single fasta entry for analysis. Each unique sequence was mapped to the reference genome (Ensembl, build 50) and reads were filtered such that only perfect alignments (full length, 100% identity) were retained. Reads that aligned to more than five positions in the genome and reads that overlapped with the UCSC RNA genes were identified and excluded from microRNA analysis.
Identification and Analysis of the Captured miRNA Sequences
Sequences that occurred 20 or more times in at least one sample were consolidated and annotated for the 31 samples. Genomic loci that overlapped with microRNAs described in miRBase (version 13) were identified as known microRNAs (see Table 31). The remaining genomic loci were identified as encoding candidate novel microRNAs (see Table 32).
The vast majority (96%) of the candidate novel microRNAs were found in more than one sample, with only a small minority of microRNAs were expressed exclusively in a specific B cell subset or malignancy. Many of the microRNAs that we identified in normal and malignant B cells were expressed at ten-fold or higher levels in these non B cell cases. These findings suggest that the microRNAs we have identified are broadly expressed and may have roles in a number of diverse tissue types.
miRNA Profiling Using Real-Time PCR
miRNA expression profiling was conducted using the Applied Biosystems 384-well multiplexed real-time PCR assay using 400 ng of total RNA. Eight reactions, each containing 50 ng of RNA and a multiplex looped primer pool with endogenous small nucleolar (sno)-RNA controls, were used to reverse-transcribe the miRNAs in parallel fashion (see Tables 33 and 34 for primers). The completed reactions were loaded onto the 384-well plate per manufacturer's instructions, and real-time PCR was run on the ABI 7900HT Prism instrument. For each 384-well plate, we used the automatically determined cycle-threshold (CT) using the SDS 2.2.1 software (Applied Biosystems). Consistent with manufacturer recommendations, a CT greater than 35 was treated as undetected. The probes deemed to be present were normalized to the average expression of a sno-RNA control. The expression values were calculated as 2−ΔCT, then median centered to 500 and log 2-transformed.
For further validation of the deep sequencing results, we selected candidate novel microRNAs (see Table 34) that were detectably measured in at the sequencing data from least one of four diffuse large B cell lymphoma (DLBCL) cases. Using stem-loop reverse transcription (Ashburner, M., et al., Nat Genet. (2000); 25(1):25-29) for quantitative PCR, we tested the expression of the candidate microRNAs in 101 primary tumors from patients with DLBCL and found that about 92% were detectably measured by real-time PCR in at least 10% of these cases, suggesting that real-time PCR reproducibly identifies microRNAs that are expressed in lymphomas. We also used real-time PCR to measure the expression of known microRNAs (see Table 33) in the same 101 samples and found that over 90% of these were also detected in at least 10% of the cases using real-time PCR. We found that six of the seven RT-PCR constructs that targeted RNA hairpins that had low probability of being a microRNA resulted in no detectable signal. These results suggest that our assays have high specificity for microRNAs and that the computational predictions based on our sequencing data correctly identified microRNAs.
Differentiating ABC DLBCL Vs. GCB DLBCL Malignancies
Gene expression profiling of patients with DLBCL has demonstrated that the tumors comprise at least two distinct diseases with different response rates to standard chemotherapy regimens (Chen, C., et al., Nucleic Acids Res. (2005); 33(20):e179). We hypothesized that microRNAs might be used to make this clinically important distinction for which gene expression profiling remains the gold standard. We performed gene expression profiling on 101 DLBCL cases and further subdivided these cases into the molecular subgroups.
Tumor samples from 101 patients with diffuse large B cell lymphoma were obtained at the time of diagnosis and freshly frozen. These cases were profiled using Affymetrix Gene 1.0 ST arrays. The molecular subgroups were distinguished using a Bayesian approach described previously (Ambros, V., et al., RNA (2003);9(3):277-279).
We found that 25 microRNAs with the highest t-statistic were equally efficacious as the gene expression profiling in differentiating the two groups of DLBCL with over 95% overlap between the classifications rendered by the two methods, using leave out one cross-validation (see Table 35). Interestingly, a subset of these 25 predictor microRNAs was candidate novel microRNAs, suggesting a biological and clinical relevance for these candidate novel microRNAs in DLBCL tumors.
Our work provides an exhaustive identification of the microRNAs in normal and malignant B cells; that is a prerequisite to the delineation of their role. Further, we have developed a comprehensive framework that spans the identification of microRNAs from deep sequencing data to measuring their expression using real-time PCR and validating their expression in primary human tumors.
It is also conceivable that some of the low-abundance microRNAs that we have identified in our study may be expressed at higher levels in other development stages or in other cell types. This notion is confirmed by our examination of the novel microRNAs in non B cell data. For instance, a number of the microRNAs that we discovered were also present at 10-fold or higher levels in cell lines derived from breast cancer and cervical cancer, suggesting that the microRNAs that we have discovered in B cells have broad biological significance.
Deep Sequencing Reveals a Novel miRNA Cluster that Regulates the TGF-β Pathway
Although microRNAs appear to be distributed throughout the genome, a number of microRNAs have been found in clusters such as miR-17-92 that are transcribed from a single primary transcript and cleaved into the individual microRNAs by the enzyme DROSHA. We found 2 separate clusters of candidate novel microRNAs on chromosome 9 and chromosome 14 (within the IgH locus), respectively. The first cluster was previously annotated as a hypothetical gene LOC100130622, and subsequently discarded from Refseq when no associated protein was identified. Our data demonstrate that this cluster (miR-2355), conserved only in primates, encodes 6 separate microRNAs: has-miR-2355a-1, has-miR-2356-1, has-miR-2355a-2, has-miR-2356-2, hsa-miR2355a-3, and hsa-miR-2355b (see Table 32 for sequence).
In order to evaluate whether the microRNAs encoded in these clusters originate from the same primary transcript, we took KMS12 multiple myeloma cells which express these microRNAs and used siRNA to knock-down the expression of the microRNA processing enzyme Drosha. This enzyme acts at the first step of microRNA processing by cleaving microRNA precursors from the primary transcript. We found that decreased Drosha expression was associated with increased accumulation of primary transcripts of both the miR-17-92 cluster as well as the novel miR-2355 cluster. MicroRNAs from miR-2355 cluster were found to be expressed more highly in normal germinal center (GC) B cells compared to memory cells.
The microRNAs of this cluster all share the same seed sequence, suggesting that they target the same genes. Among the computationally predicted targets of this microRNA cluster, we identified SMAD2 and SMAD3 which are well known mediators of the TGF-beta signaling pathway. We noted that gene expression of both SMAD2 and SMAD3 in our set of 101 DLBCLs were inversely correlated with this cluster (P<0.001, correlation test). Gene set enrichment analysis revealed that expression of the TGF-beta pathway in DLBCL samples varied inversely with the expression of the microRNA cluster, with a higher expression of the microRNA associated with a lower expression of the pathway (P<10−6), which has been noted as a factor in the biology of these tumors.
TABLE 31
miRNAs identified by deep sequencing analysis.
Putative
Mature/ miRBase miRBase
SEQ Minor Mature/ Mature/Minor miRBase SEQ
ID Mature Sequence miRNA miRBase Minor Accession Mature/Minor ID
NO. Captured ID ID ID Number Sequence NO.
1 CUGCGCAAGCUACUGCCUUGCU hsa-let- hsa-let- hsa-let- MIMAT0004585 CUGCGCAAGCUACUGCCUUGCU 393
7i* 7i 7i*
2 CCACGGAUGUUUGAGCAUGUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0004516 ACGGAUGUUUGAGCAUGUGCUA 394
105-1* 105-1 105*
3 CCACGGAUGUUUGAGCAUGUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0004516 ACGGAUGUUUGAGCAUGUGCUA 395
105-2* 105-2 105*
4 UACUGCAAUGUAAGCACUUCUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004517 CUGCAAUGUAAGCACUUCUUAC 396
106a* 106a 106a*
5 CAUUAUUACUUUUGGUACGCG hsa-mir- hsa-mir- hsa-miR- MIMAT0000444 CAUUAUUACUUUUGGUACGCG 397
126* 126 126*
6 AAGCCCUUACCCCAAAAAGUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0004548 AAGCCCUUACCCCAAAAAGUAU 398
129* 129-1 129*
7 AAGCCCUUACCCCAAAAAGCAU hsa-mir- hsa-mir- hsa-miR- MIMAT0004605 AAGCCCUUACCCCAAAAAGCAU 399
129-2* 129-2 129-3p
8 UCUACAGUGCACGUGUCUCCAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000250 UCUACAGUGCACGUGUCUCCAG 400
139-5p 139 139-5p
9 CUGGUACAGGCCUGGGGGACAG hsa-mir- hsa-mir- hsa-miR- MIMAT0004610 CUGGUACAGGCCUGGGGGACAG 401
150* 150 150*
10 ACUGCAGUGAAGGCACUUGUAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000071 ACUGCAGUGAAGGCACUUGUAG 402
17* 17 17*
11 UGAAUUACCGAAGGGCCAUAA hsa-mir- hsa-mir- hsa-miR- MIMAT0004560 GUGAAUUACCGAAGGGCCAUAA 403
183* 183 183*
12 AGGGGCUGGCUUUCCUCUGGUC hsa-mir- hsa-mir- hsa-miR- MIMAT0004611 AGGGGCUGGCUUUCCUCUGGUC 404
185* 185 185*
13 ACUGCCCUAAGUGCUCCUUCUGG hsa-mir- hsa-mir- hsa-miR- MIMAT0002891 ACUGCCCUAAGUGCUCCUUCUGG 405
18a* 18a 18a*
14 AACUGGCCUACAAAGUCCCAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000459 AACUGGCCUACAAAGUCCCAGU 406
193a-3p 193a 193a-3p
15 CGGGGUUUUGAGGGCGAGAUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004767 CGGGGUUUUGAGGGCGAGAUGA 407
193b* 193b 193b*
16 CCCAGUGUUCAGACUACCUGUUC hsa-mir- hsa-mir- hsa-miR- MIMAT0000231 CCCAGUGUUCAGACUACCUGUUC 408
199a-2* 199a-2 199a-5p
17 UAGUUUUGCAUAGUUGCACUAC hsa-mir- hsa-mir- hsa-miR- MIMAT0004490 AGUUUUGCAUAGUUGCACUACA 409
19a* 19a 19a*
18 AGUUUUGCAGGUUUGCAUCCAGC hsa-mir- hsa-mir- hsa-miR- MIMAT0004491 AGUUUUGCAGGUUUGCAUCCAGC 410
19b-1* 19b-1 19b-1*
19 ACUGUAGUAUGGGCACUUCCAG hsa-mir- hsa-mir- hsa-miR- MIMAT0004752 ACUGUAGUAUGGGCACUUCCAG 411
20b* 20b 20b*
20 ACCUGGCAUACAAUGUAGAUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004568 ACCUGGCAUACAAUGUAGAUUU 412
221* 221 221*
21 GGGUUCCUGGCAUGCUGAUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004587 UGGGUUCCUGGCAUGCUGAUUU 413
23b* 23b 23b*
22 AGGCGGAGACUUGGGCAAUUG hsa-mir- hsa-mir- hsa-miR- MIMAT0004498 AGGCGGAGACUUGGGCAAUUG 414
25* 25 25*
23 AGAGCUUAGCUGAUUGGUGAAC hsa-mir- hsa-mir- hsa-miR- MIMAT0004588 AGAGCUUAGCUGAUUGGUGAAC 415
27b* 27b 27b*
24 CUGGGAGGUGGAUGUUUACUUC hsa-mir- hsa-mir- hsa-miR- MIMAT0004589 CUGGGAGGUGGAUGUUUACUUC 416
30b* 30b 30b*
25 CUGGGAGAGGGUUGUUUACUCC hsa-mir- hsa-mir- hsa-miR- MIMAT0004674 CUGGGAGAGGGUUGUUUACUCC 417
30c-1* 30c-1 30c-1*
26 CUGGGAGAAGGCUGUUUACUCU hsa-mir- hsa-mir- hsa-miR- MIMAT0004550 CUGGGAGAAGGCUGUUUACUCU 418
30c-2* 30c-2 30c-2*
27 CUUUCAGUCGGAUGUUUACAGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000693 CUUUCAGUCGGAUGUUUACAGC 419
30e* 30e 30e*
28 UCCCUGUCCUCCAGGAGCUCACG hsa-mir- hsa-mir- hsa-miR- MIMAT0000764 UCCCUGUCCUCCAGGAGCUCACG 420
339-5p 339 339-5p
29 UCUCACACAGAAAUCGCACCCGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000753 UCUCACACAGAAAUCGCACCCGU 421
342-3p 342 342-3p
30 AAUCAGCAAGUAUACUGCCCUA hsa-mir- hsa-mir- hsa-miR- MIMAT0004557 CAAUCAGCAAGUAUACUGCCCU 422
34a* 34a 34a*
31 UCCCCCAGGUGUGAUUCUGAUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004682 UCCCCCAGGUGUGAUUCUGAUUU 423
361-3p 361 361-3p
32 AAUCCUUGGAACCUAGGUGUGAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000705 AAUCCUUGGAACCUAGGUGUGAGU 424
362-5p 362 362-5p
33 AGGGACUUUCAGGGGCAGCUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0009199 AGGGACUUUCAGGGGCAGCUGU 425
365-2* 365-2 365*
34 CUUAUCAGAUUGUAUUGUAAUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004688 CUUAUCAGAUUGUAUUGUAAUU 426
374a* 374a 374a*
35 CUUAGCAGGUUGUAUUAUCAUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004956 CUUAGCAGGUUGUAUUAUCAUU 427
374b* 374b 374b*
36 AGGUUACCCGAGCAACUUUGCAU hsa-mir- hsa-mir- hsa-miR- MIMAT0001638 AGGUUACCCGAGCAACUUUGCAU 428
409-5p 409 409-5p
37 CAAAACGUGAGGCGCUGCUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0004749 CAAAACGUGAGGCGCUGCUAU 429
424* 424 424*
38 UAUGUGCCUUUGGACUACAUCG hsa-mir- hsa-mir- hsa-miR- MIMAT0003150 UAUGUGCCUUUGGACUACAUCG 430
455-5p 455 455-5p
39 GUCAUACACGGCUCUCCUCUCU hsa-mir- hsa-mir- hsa-miR- MIMAT0002176 GUCAUACACGGCUCUCCUCUCU 431
485-3p 485 485-3p
40 UGUCUUACUCCCUCAGGCACAU hsa-mir- hsa-mir- hsa-miR- MIMAT0003257 UGUCUUACUCCCUCAGGCACAU 432
550-1* 550-1 550*
41 UGUCUUACUCCCUCAGGCACAU hsa-mir- hsa-mir- hsa-miR- MIMAT0003257 UGUCUUACUCCCUCAGGCACAU 433
550-2* 550-2 550*
42 GAAAUCAAGCGUGGGUGAGACC hsa-mir- hsa-mir- hsa-miR- MIMAT0004794 GAAAUCAAGCGUGGGUGAGACC 434
551b* 551b 551b*
43 AUUCUAAUUUCUCCACGUCUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0003241 AUUCUAAUUUCUCCACGUCUUU 435
576-5p 576 576-5p
44 UCAGAACAAAUGCCGGUUCCCAGA hsa-mir- hsa-mir- hsa-miR- MIMAT0003256 UCAGAACAAAUGCCGGUUCCCAGA 436
589* 589 589*
45 GAGCUUAUUCAUAAAAGUGCAG hsa-mir- hsa-mir- hsa-miR- MIMAT0003258 GAGCUUAUUCAUAAAAGUGCAG 437
590-5p 590 590-5p
46 GGGGGUCCCCGGUGCUCGGAUC hsa-mir- hsa-mir- hsa-miR- MIMAT0004804 GGGGGUCCCCGGUGCUCGGAUC 438
615-5p 615 615-5p
47 GACUAUAGAACUUUCCCCCUCA hsa-mir- hsa-mir- hsa-miR- MIMAT0004808 GACUAUAGAACUUUCCCCCUCA 439
625* 625 625*
48 UGGUGGGCCGCAGAACAUGUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0003330 UGGUGGGCCGCAGAACAUGUGC 440
654-5p 654 654-5p
49 AGGAAGCCCUGGAGGGGCUGGAG hsa-mir- hsa-mir- hsa-miR- MIMAT0003880 AGGAAGCCCUGGAGGGGCUGGAG 441
671-5p 671 671-5p
50 AACUAGACUGUGAGCUUCUAGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004927 CAACUAGACUGUGAGCUUCUAG 442
708* 708 708*
51 CAACAAAUCACAGUCUGCCAUA hsa-mir- hsa-mir- hsa-miR- MIMAT0004553 CAACAAAUCACAGUCUGCCAUA 443
7-1* 7-1 7-1*
52 AUAAAGCUAGAUAACCGAAAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000442 AUAAAGCUAGAUAACCGAAAGU 444
9-1* 9-1 9*
53 AUAAAGCUAGAUAACCGAAAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000442 AUAAAGCUAGAUAACCGAAAGU 445
9-2* 9-2 9*
54 AGGGACGGGACGCGGUGCAGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0004792 AGGGACGGGACGCGGUGCAGUG 446
92b* 92b 92b*
55 AUAAAGCUAGAUAACCGAAAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000442 AUAAAGCUAGAUAACCGAAAGU 447
9-3* 9-3 9*
56 UGAGGUAGUAGGUUGUAUAGUU hsa-let- hsa-let- hsa-let- MIMAT0000062 UGAGGUAGUAGGUUGUAUAGUU 448
7a-2 7a-2 7a
57 UGAGGUAGUAGGUUGUAUAGUU hsa-let- hsa-let- hsa-let- MIMAT0000062 UGAGGUAGUAGGUUGUAUAGUU 449
7a-3 7a-3 7a
58 UGAGGUAGUAGGUUGUAUGGUU hsa-let- hsa-let- hsa-let- MIMAT0000064 UGAGGUAGUAGGUUGUAUGGUU 450
7c 7c 7c
59 UGAGGUAGGAGGUUGUAUAGUU hsa-let- hsa-let- hsa-let- MIMAT0000066 UGAGGUAGGAGGUUGUAUAGUU 451
7e 7e 7e
60 UGAGGUAGUAGAUUGUAUAGUU hsa-let- hsa-let- hsa-let- MIMAT0000067 UGAGGUAGUAGAUUGUAUAGUU 452
7f-1 7f-1 7f
61 UGAGGUAGUAGUUUGUACAGUU hsa-let- hsa-let- hsa-let- MIMAT0000414 UGAGGUAGUAGUUUGUACAGUU 453
7g 7g 7g
62 UGAGGUAGUAGUUUGUGCUGUU hsa-let- hsa-let- hsa-let- MIMAT0000415 UGAGGUAGUAGUUUGUGCUGUU 454
7i 7i 7i
63 AACCCGUAGAUCCGAACUUGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000098 AACCCGUAGAUCCGAACUUGUG 455
100 100 100
64 UACAGUACUGUGAUAACUGAA hsa-mir- hsa-mir- hsa-miR- MIMAT0000099 UACAGUACUGUGAUAACUGAA 456
101-1 101-1 101
65 GUACAGUACUGUGAUAACUGAA hsa-mir- hsa-mir- hsa-miR- MIMAT0000099 UACAGUACUGUGAUAACUGAA 457
101-2 101-2 101
66 AGCAGCAUUGUACAGGGCUAUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000101 AGCAGCAUUGUACAGGGCUAUGA 458
103-1 103-1 103
67 AGCAGCAUUGUACAGGGCUAUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000101 AGCAGCAUUGUACAGGGCUAUGA 4589
103-2 103-2 103
68 AAAUGCUCAGACUCCUGUGGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000102 UCAAAUGCUCAGACUCCUGUGGU 460
105-1 105-1 105
69 AAAUGCUCAGACUCCUGUGGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000102 UCAAAUGCUCAGACUCCUGUGGU 461
105-2 105-2 105
70 AAAAGUGCUUACAGUGCAGGUAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000103 AAAAGUGCUUACAGUGCAGGUAG 462
106a 106a 106a
71 UAAAGUGCUGACAGUGCAGAU hsa-mir- hsa-mir- hsa-miR- MIMAT0000680 UAAAGUGCUGACAGUGCAGAU 463
106b 106b 106b
72 AGCAGCAUUGUACAGGGCUAUCA hsa-mir- hsa-mir- hsa-miR- MIMAT0000104 AGCAGCAUUGUACAGGGCUAUCA 464
107 107 107
73 UACCCUGUAGAUCCGAAUUUGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000253 UACCCUGUAGAUCCGAAUUUGUG 465
10a 10a 10a
74 UACCCUGUAGAACCGAAUUUGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000254 UACCCUGUAGAACCGAAUUUGUG 466
10b 10b 10b
75 UGGAAUGUAAAGAAGUAUGUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0000416 UGGAAUGUAAAGAAGUAUGUAU 467
1-1 1-1 1
76 UGGAAUGUAAAGAAGUAUGUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0000416 UGGAAUGUAAAGAAGUAUGUAU 468
1-2 1-2 1
77 UGGAGUGUGACAAUGGUGUUUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000421 UGGAGUGUGACAAUGGUGUUUG 469
122 122 122
78 UAAGGCACGCGGUGAAUGCC hsa-mir- hsa-mir- hsa-miR- MIMAT0000422 UAAGGCACGCGGUGAAUGCC 470
124-1 124-1 124
79 UAAGGCACGCGGUGAAUGCC hsa-mir- hsa-mir- hsa-miR- MIMAT0000422 UAAGGCACGCGGUGAAUGCC 471
124-2 124-2 124
80 UAAGGCACGCGGUGAAUGCC hsa-mir- hsa-mir- hsa-miR- MIMAT0000422 UAAGGCACGCGGUGAAUGCC 472
124-3 124-3 124
81 ACCCGUCCCGUUCGUCCCCGGA hsa-mir- hsa-mir- hsa-miR- MIMAT0005899 ACCCGUCCCGUUCGUCCCCGGA 473
1247 1247 1247
82 ACGGUGCUGGAUGUGGCCUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0005902 ACGGUGCUGGAUGUGGCCUUU 474
1250 1250 1250
83 AGAAGGAAAUUGAAUUCAUUUA hsa-mir- hsa-mir- hsa-miR- MIMAT0005944 AGAAGGAAAUUGAAUUCAUUUA 475
1252 1252 1252
84 AGCCUGGAAGCUGGAGCCUGCAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0005905 AGCCUGGAAGCUGGAGCCUGCAGU 476
1254 1254 1254
85 AGGAUGAGCAAAGAAAGUAGAUU hsa-mir- hsa-mir- hsa-miR- MIMAT0005906 AGGAUGAGCAAAGAAAGUAGAUU 477
1255a 1255a 1255a
86 AGGCAUUGACUUCUCACUAGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0005907 AGGCAUUGACUUCUCACUAGCU 478
1256 1256 1256
87 CGUACCGUGAGUAAUAAUGCG hsa-mir- hsa-mir- hsa-miR- MIMAT0000445 UCGUACCGUGAGUAAUAAUGCG 479
126 126 126
88 AUGGGUGAAUUUGUAGAAGGAU hsa-mir- hsa-mir- hsa-miR- MIMAT0005914 AUGGGUGAAUUUGUAGAAGGAU 480
1262 1262 1262
89 AUGGUACCCUGGCAUACUGAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0005915 AUGGUACCCUGGCAUACUGAGU 481
1263 1263 1263
90 CAGGAUGUGGUCAAGUGUUGUU hsa-mir- hsa-mir- hsa-miR- MIMAT0005918 CAGGAUGUGGUCAAGUGUUGUU 482
1265 1265 1265
91 CCUCAGGGCUGUAGAACAGGGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0005920 CCUCAGGGCUGUAGAACAGGGCU 483
1266 1266 1266
92 CUGGACUGAGCCGUGCUACUGG hsa-mir- hsa-mir- hsa-miR- MIMAT0005923 CUGGACUGAGCCGUGCUACUGG 484
1269 1269 1269
93 CUGGAGAUAUGGAAGAGCUGUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0005924 CUGGAGAUAUGGAAGAGCUGUGU 485
1270 1270 1270
94 CUUGGCACCUAGCAAGCACUCA hsa-mir- hsa-mir- hsa-miR- MIMAT0005796 CUUGGCACCUAGCAAGCACUCA 486
1271 1271 1271
95 UCGGAUCCGUCUGAGCUUGGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000446 UCGGAUCCGUCUGAGCUUGGCU 487
127-3p 127 127-3p
96 UACGUAGAUAUAUAUGUAUUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0005933 UACGUAGAUAUAUAUGUAUUUU 488
1277 1277 1277
97 UAGUACUGUGCAUAUCAUCUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0005936 UAGUACUGUGCAUAUCAUCUAU 489
1278 1278 1278
98 UCACAGUGAACCGGUCUCUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0000424 UCACAGUGAACCGGUCUCUUU 490
128-1 128-1 128
99 UCACAGUGAACCGGUCUCUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0000424 UCACAGUGAACCGGUCUCUUU 491
128-2 128-2 128
100 CUUUUUGCGGUCUGGGCUUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000242 CUUUUUGCGGUCUGGGCUUGC 492
129-1 129-1 129-5p
101 CUUUUUGCGGUCUGGGCUUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000242 CUUUUUGCGGUCUGGGCUUGC 493
129-2 129-2 129-5p
102 UGUGAGGUUGGCAUUGUUGUCU hsa-mir- hsa-mir- hsa-miR- MIMAT0005884 UGUGAGGUUGGCAUUGUUGUCU 494
1294 1294 1294
103 UUAGGCCGCAGAUCUGGGUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0005885 UUAGGCCGCAGAUCUGGGUGA 495
1295 1295 1295
104 UUCAUUCGGCUGUCCAGAUGUA hsa-mir- hsa-mir- hsa-miR- MIMAT0005800 UUCAUUCGGCUGUCCAGAUGUA 496
1298 1298 1298
105 UUGCAGCUGCCUGGGAGUGACUUC hsa-mir- hsa-mir- hsa-miR- MIMAT0005797 UUGCAGCUGCCUGGGAGUGACUUC 497
1301 1301 1301
106 CGGUUUGAGGCUACAGUGAGAU hsa-mir- hsa-mir- hsa-miR- MIMAT0005892 UUUGAGGCUACAGUGAGAUGUG 498
1304 1304 1304
107 ACGUUGGCUCUGGUGGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0005950 ACGUUGGCUCUGGUGGUG 499
1306 1306 1306
108 ACUCGGCGUGGCGUCGGUCGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0005951 ACUCGGCGUGGCGUCGGUCGUG 500
1307 1307 1307
109 CAGUGCAAUGUUAAAAGGGCAU hsa-mir- hsa-mir- hsa-miR- MIMAT0000425 CAGUGCAAUGUUAAAAGGGCAU 501
130a 130a 130a
110 CAGUGCAAUGAUGAAAGGGCAU hsa-mir- hsa-mir- hsa-miR- MIMAT0000691 CAGUGCAAUGAUGAAAGGGCAU 502
130b 130b 130b
111 UAACAGUCUACAGCCAUGGUCG hsa-mir- hsa-mir- hsa-miR- MIMAT0000426 UAACAGUCUACAGCCAUGGUCG 503
132 132 132
112 ACCGUGGCUUUCGAUUGUUACU hsa-mir- hsa-mir- hsa-miR- MIMAT0004594 ACCGUGGCUUUCGAUUGUUACU 504
132* 132 132*
113 UGUGACUGGUUGACCAGAGGGG hsa-mir- hsa-mir- hsa-miR- MIMAT0000447 UGUGACUGGUUGACCAGAGGGG 505
134 134 134
114 UAUGGCUUUUCAUUCCUAUGUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000758 UAUGGCUUUUCAUUCCUAUGUGA 506
135b 135b 135b
115 ACUCCAUUUGUUUUGAUGAUGGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000448 ACUCCAUUUGUUUUGAUGAUGGA 507
136 136 136
116 AGCUGGUGUUGUGAAUCAGGCCG hsa-mir- hsa-mir- hsa-miR- MIMAT0000430 AGCUGGUGUUGUGAAUCAGGCCG 508
138-1 138-1 138
117 AGCUGGUGUUGUGAAUCAGGCCG hsa-mir- hsa-mir- hsa-miR- MIMAT0000430 AGCUGGUGUUGUGAAUCAGGCCG 509
138-2 138-2 138
118 UGGAGACGCGGCCCUGUUGGAG hsa-mir- hsa-mir- hsa-miR- MIMAT0004552 GGAGACGCGGCCCUGUUGGAGU 510
139-3p 139 139-3p
119 UACCACAGGGUAGAACCACGG hsa-mir- hsa-mir- hsa-miR- MIMAT0004597 UACCACAGGGUAGAACCACGG 511
140-3p 140 140-3p
120 UAACACUGUCUGGUAAAGAUGG hsa-mir- hsa-mir- hsa-miR- MIMAT0000432 UAACACUGUCUGGUAAAGAUGG 512
141 141 141
121 CCCAUAAAGUAGAAAGCACU hsa-mir- hsa-mir- hsa-miR- MIMAT0000433 CAUAAAGUAGAAAGCACUACU 513
142 142 142-5p
122 UGAGAUGAAGCACUGUAGCUC hsa-mir- hsa-mir- hsa-miR- MIMAT0000435 UGAGAUGAAGCACUGUAGCUC 514
143 143 143
123 GGAUAUCAUCAUAUACUGUAAG hsa-mir- hsa-mir- hsa-miR- MIMAT0004600 GGAUAUCAUCAUAUACUGUAAG 515
144 144 144*
124 GUCCAGUUUUCCCAGGAAUCCCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000437 GUCCAGUUUUCCCAGGAAUCCCU 516
145 145 145
125 UGAGAACUGAAUUCCAUGGGUU hsa-mir- hsa-mir- hsa-miR- MIMAT0000449 UGAGAACUGAAUUCCAUGGGUU 517
146a 146a 146a
126 UGAGAACUGAAUUCCAUAGGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0002809 UGAGAACUGAAUUCCAUAGGCU 518
146b 146b 146b-5p
127 UCAGUGCACUACAGAACUUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000243 UCAGUGCACUACAGAACUUUGU 519
148a 148a 148a
128 UCAGUGCAUCACAGAACUUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000759 UCAGUGCAUCACAGAACUUUGU 520
148b 148b 148b
129 UCUCCCAACCCUUGUACCAGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000451 UCUCCCAACCCUUGUACCAGUG 521
150 150 150
130 CUAGACUGAAGCUCCUUGAGG hsa-mir- hsa-mir- hsa-miR- MIMAT0000757 CUAGACUGAAGCUCCUUGAGG 522
151-3p 151 151-3p
131 UCAGUGCAUGACAGAACUUGG hsa-mir- hsa-mir- hsa-miR- MIMAT0000438 UCAGUGCAUGACAGAACUUGG 523
152 152 152
132 UUAAUGCUAAUCGUGAUAGGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000646 UUAAUGCUAAUCGUGAUAGGGGU 524
155 155 155
133 UAGCAGCACAUAAUGGUUUGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000068 UAGCAGCACAUAAUGGUUUGUG 525
15a 15a 15a
134 UAGCAGCACAUCAUGGUUUACA hsa-mir- hsa-mir- hsa-miR- MIMAT0000417 UAGCAGCACAUCAUGGUUUACA 526
15b 15b 15b
135 UAGCAGCACGUAAAUAUUGGCG hsa-mir- hsa-mir- hsa-miR- MIMAT0000069 UAGCAGCACGUAAAUAUUGGCG 527
16-1 16-1 16
136 UAGCAGCACGUAAAUAUUGGCG hsa-mir- hsa-mir- hsa-miR- MIMAT0000069 UAGCAGCACGUAAAUAUUGGCG 528
16-2 16-2 16
137 CAAAGUGCUUACAGUGCAGGUAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000070 CAAAGUGCUUACAGUGCAGGUAG 529
17 17 17
138 AACAUUCAACGCUGUCGGUGAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000256 AACAUUCAACGCUGUCGGUGAGU 530
181a-1 181a-1 181a
139 AACAUUCAACGCUGUCGGUGAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000256 AACAUUCAACGCUGUCGGUGAGU 531
181a-2 181a-2 181a
140 AACAUUCAUUGCUGUCGGUGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000257 AACAUUCAUUGCUGUCGGUGGGU 532
181b-1 181b-1 181b
141 AACAUUCAUUGCUGUCGGUGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000257 AACAUUCAUUGCUGUCGGUGGGU 533
181b-2 181b-2 181b
142 AACAUUCAACCUGUCGGUGAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000258 AACAUUCAACCUGUCGGUGAGU 534
181c 181c 181c
143 AACAUUCAUUGUUGUCGGUGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0002821 AACAUUCAUUGUUGUCGGUGGGU 535
181d 181d 181d
144 UUUGGCAAUGGUAGAACUCACACU hsa-mir- hsa-mir- hsa-miR- MIMAT0000259 UUUGGCAAUGGUAGAACUCACACU 536
182 182 182
145 UAUGGCACUGGUAGAAUUCACU hsa-mir- hsa-mir- hsa-miR- MIMAT0000261 UAUGGCACUGGUAGAAUUCACU 537
183 183 183
146 UGGACGGAGAACUGAUAAGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000454 UGGACGGAGAACUGAUAAGGGU 538
184 184 184
147 UGGAGAGAAAGGCAGUUCCUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000455 UGGAGAGAAAGGCAGUUCCUGA 539
185 185 185
148 CAAAGAAUUCUCCUUUUGGGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000456 CAAAGAAUUCUCCUUUUGGGCU 540
186 186 186
149 CAUCCCUUGCAUGGUGGAGGG hsa-mir- hsa-mir- hsa-miR- MIMAT0000457 CAUCCCUUGCAUGGUGGAGGG 541
188-5p 188 188-5p
150 UAAGGUGCAUCUAGUGCAGAUAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000072 UAAGGUGCAUCUAGUGCAGAUAG 542
18a 18a 18a
151 CAACGGAAUCCCAAAAGCAGCUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000440 CAACGGAAUCCCAAAAGCAGCUG 543
191 191 191
152 CUGACCUAUGAAUUGACAGCC hsa-mir- hsa-mir- hsa-miR- MIMAT0000222 CUGACCUAUGAAUUGACAGCC 544
192 192 192
153 UGGGUCUUUGCGGGCGAGAUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004614 UGGGUCUUUGCGGGCGAGAUGA 545
193a-5p 193a 193a-5p
154 AACUGGCCCUCAAAGUCCCGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0002819 AACUGGCCCUCAAAGUCCCGCU 546
193b 193b 193b
155 UGUAACAGCAACUCCAUGUGGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000460 UGUAACAGCAACUCCAUGUGGA 547
194-1 194-1 194
156 UAGCAGCACAGAAAUAUUGGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000461 UAGCAGCACAGAAAUAUUGGC 548
195 195 195
157 UAGGUAGUUUCAUGUUGUUGGG hsa-mir- hsa-mir- hsa-miR- MIMAT0000226 UAGGUAGUUUCAUGUUGUUGGG 549
196a-1 196a-1 196a
158 UAGGUAGUUUCAUGUUGUUGGG hsa-mir- hsa-mir- hsa-miR- MIMAT0000226 UAGGUAGUUUCAUGUUGUUGGG 550
196a-2 196a-2 196a
159 UAGGUAGUUUCCUGUUGUUGGG hsa-mir- hsa-mir- hsa-miR- MIMAT0001080 UAGGUAGUUUCCUGUUGUUGGG 551
196b 196b 196b
160 UUCACCACCUUCUCCACCCAGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000227 UUCACCACCUUCUCCACCCAGC 552
197 197 197
161 ACAGUAGUCUGCACAUUGGUUA hsa-mir- hsa-mir- hsa-miR- MIMAT0000232 ACAGUAGUCUGCACAUUGGUUA 553
199a-1 199a-1 199a-3p
162 ACAGUAGUCUGCACAUUGGUUA hsa-mir- hsa-mir- hsa-miR- MIMAT0000232 ACAGUAGUCUGCACAUUGGUUA 554
199a-2 199a-2 199a-3p
163 ACAGUAGUCUGCACAUUGGUUA hsa-mir- hsa-mir- hsa-miR- MIMAT0004563 ACAGUAGUCUGCACAUUGGUUA 555
199b 199b 199b-3p
164 UGUGCAAAUCUAUGCAAAACUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000073 UGUGCAAAUCUAUGCAAAACUGA 556
19a 19a 19a
165 UGUGCAAAUCCAUGCAAAACUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000074 UGUGCAAAUCCAUGCAAAACUGA 557
19b-1 19b-1 19b
166 UGUGCAAAUCCAUGCAAAACUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000074 UGUGCAAAUCCAUGCAAAACUGA 558
19b-2 19b-2 19b
167 UAACACUGUCUGGUAACGAUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000682 UAACACUGUCUGGUAACGAUGU 559
200a 200a 200a
168 UAAUACUGCCUGGUAAUGAUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000318 UAAUACUGCCUGGUAAUGAUGA 560
200b 200b 200b
169 UAAUACUGCCGGGUAAUGAUGGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000617 UAAUACUGCCGGGUAAUGAUGGA 561
200c 200c 200c
170 UUCCUAUGCAUAUACUUCUUUG hsa-mir- hsa-mir- hsa-miR- MIMAT0002810 UUCCUAUGCAUAUACUUCUUUG 562
202* 202 202*
171 GUGAAAUGUUUAGGACCACUAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000264 GUGAAAUGUUUAGGACCACUAG 563
203 203 203
172 UUCCCUUUGUCAUCCUAUGCCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000265 UUCCCUUUGUCAUCCUAUGCCU 564
204 204 204
173 UCCUUCAUUCCACCGGAGUCUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000266 UCCUUCAUUCCACCGGAGUCUG 565
205 205 205
174 UGGAAUGUAAGGAAGUGUGUGG hsa-mir- hsa-mir- hsa-miR- MIMAT0000462 UGGAAUGUAAGGAAGUGUGUGG 566
206 206 206
175 UAAAGUGCUUAUAGUGCAGGUAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000075 UAAAGUGCUUAUAGUGCAGGUAG 567
20a 20a 20a
176 CAAAGUGCUCAUAGUGCAGGUAG hsa-mir- hsa-mir- hsa-miR- MIMAT0001413 CAAAGUGCUCAUAGUGCAGGUAG 568
20b 20b 20b
177 UAGCUUAUCAGACUGAUGUUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000076 UAGCUUAUCAGACUGAUGUUGA 569
21 21 21
178 CUGUGCGUGUGACAGCGGCUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000267 CUGUGCGUGUGACAGCGGCUGA 570
210 210 210
179 UAACAGUCUCCAGUCACGGCC hsa-mir- hsa-mir- hsa-miR- MIMAT0000269 UAACAGUCUCCAGUCACGGCC 571
212 212 212
180 ACAGCAGGCACAGACAGGCAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000271 ACAGCAGGCACAGACAGGCAGU 572
214 214 214
181 UGACCUAUGAAUUGACAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000272 AUGACCUAUGAAUUGACAGAC 573
215 215 215
182 AAAUCUCUGCAGGCAAAUGUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004959 AAAUCUCUGCAGGCAAAUGUGA 574
216b 216b 216b
183 AUACUGCAUCAGGAACUGAUUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000274 UACUGCAUCAGGAACUGAUUGGA 575
217 217 217
184 AGAGUUGAGUCUGGACGUCCCG hsa-mir- hsa-mir- hsa-miR- MIMAT0004567 AGAGUUGAGUCUGGACGUCCCG 576
219-1 219-1 219-1-3p
185 AAGCUGCCAGUUGAAGAACUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000077 AAGCUGCCAGUUGAAGAACUGU 577
22 22 22
186 AGCUACAUUGUCUGCUGGGUUUC hsa-mir- hsa-mir- hsa-miR- MIMAT0000278 AGCUACAUUGUCUGCUGGGUUUC 578
221 221 221
187 AGCUACAUCUGGCUACUGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000279 AGCUACAUCUGGCUACUGGGU 579
222 222 222
188 CGUGUAUUUGACAAGCUGAGUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004570 CGUGUAUUUGACAAGCUGAGUU 580
223* 223 223*
189 CAAGUCACUAGUGGUUCCGUU hsa-mir- hsa-mir- hsa-miR- MIMAT0000281 CAAGUCACUAGUGGUUCCGUU 581
224 224 224
190 AUCACAUUGCCAGGGAUUUCC hsa-mir- hsa-mir- hsa-miR- MIMAT0000078 AUCACAUUGCCAGGGAUUUCC 582
23a 23a 23a
191 AUCACAUUGCCAGGGAUUACC hsa-mir- hsa-mir- hsa-miR- MIMAT0000418 AUCACAUUGCCAGGGAUUACC 583
23b 23b 23b
192 UGGCUCAGUUCAGCAGGAACAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000080 UGGCUCAGUUCAGCAGGAACAG 584
24-1 24-1 24
193 UGGCUCAGUUCAGCAGGAACAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000080 UGGCUCAGUUCAGCAGGAACAG 585
24-2 24-2 24
194 CAUUGCACUUGUCUCGGUCUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000081 CAUUGCACUUGUCUCGGUCUGA 586
25 25 25
195 UUCAAGUAAUCCAGGAUAGGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000082 UUCAAGUAAUCCAGGAUAGGCU 587
26a-1 26a-1 26a
196 UUCAAGUAAUCCAGGAUAGGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000082 UUCAAGUAAUCCAGGAUAGGCU 588
26a-2 26a-2 26a
197 UUCAAGUAAUUCAGGAUAGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000083 UUCAAGUAAUUCAGGAUAGGU 589
26b 26b 26b
198 UUCACAGUGGCUAAGUUCCGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000084 UUCACAGUGGCUAAGUUCCGC 590
27a 27a 27a
199 UUCACAGUGGCUAAGUUCUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000419 UUCACAGUGGCUAAGUUCUGC 591
27b 27b 27b
200 CACUAGAUUGUGAGCUCCUGGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004502 CACUAGAUUGUGAGCUCCUGGA 592
28 28 28-3p
201 GAGGGUUGGGUGGAGGCUCUCC hsa-mir- hsa-mir- hsa-miR- MIMAT0004679 GAGGGUUGGGUGGAGGCUCUCC 593
296-3p 296 296-3p
202 AUGGUUUACCGUCCCACAUACA hsa-mir- hsa-mir- hsa-miR- MIMAT0002890 UGGUUUACCGUCCCACAUACAU 594
299-5p 299 299-5p
203 UAGCACCAUCUGAAAUCGGUUA hsa-mir- hsa-mir- hsa-miR- MIMAT0000086 UAGCACCAUCUGAAAUCGGUUA 595
29a 29a 29a
204 UAGCACCAUUUGAAAUCAGUGUU hsa-mir- hsa-mir- hsa-miR- MIMAT0000100 UAGCACCAUUUGAAAUCAGUGUU 596
29b-1 29b-1 29b
205 UAGCACCAUUUGAAAUCAGUGUU hsa-mir- hsa-mir- hsa-miR- MIMAT0000100 UAGCACCAUUUGAAAUCAGUGUU 597
29b-2 29b-2 29b
206 UAGCACCAUUUGAAAUCGGUUA hsa-mir- hsa-mir- hsa-miR- MIMAT0000681 UAGCACCAUUUGAAAUCGGUUA 598
29c 29c 29c
207 CAGUGCAAUGAUAUUGUCAAAGC hsa-mir- hsa-mir- hsa-miR- MIMAT0004958 CAGUGCAAUGAUAUUGUCAAAGC 599
301b 301b 301b
208 UGUAAACAUCCUCGACUGGAAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000087 UGUAAACAUCCUCGACUGGAAG 600
30a 30a 30a
209 UGUAAACAUCCUACACUCAGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000420 UGUAAACAUCCUACACUCAGCU 601
30b 30b 30b
210 UGUAAACAUCCUACACUCUCAGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000244 UGUAAACAUCCUACACUCUCAGC 602
30c-1 30c-1 30c
211 UGUAAACAUCCUACACUCUCAGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000244 UGUAAACAUCCUACACUCUCAGC 603
30c-2 30c-2 30c
212 UGUAAACAUCCCCGACUGGAAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000245 UGUAAACAUCCCCGACUGGAAG 604
30d 30d 30d
213 UGUAAACAUCCUUGACUGGAAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000692 UGUAAACAUCCUUGACUGGAAG 605
30e 30e 30e
214 AGGCAAGAUGCUGGCAUAGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000089 AGGCAAGAUGCUGGCAUAGCU 606
31 31 31
215 UAUUGCACAUUACUAAGUUGCA hsa-mir- hsa-mir- hsa-miR- MIMAT0000090 UAUUGCACAUUACUAAGUUGCA 607
32 32 32
216 AAAAGCUGGGUUGAGAGGGCGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000510 AAAAGCUGGGUUGAGAGGGCGA 608
320a 320a 320a
217 AAAAGCUGGGUUGAGAGGGCAA hsa-mir- hsa-mir- hsa-miR- MIMAT0005792 AAAAGCUGGGUUGAGAGGGCAA 609
320b-1 320b-1 320b
218 AAAAGCUGGGUUGAGAGGGCAA hsa-mir- hsa-mir- hsa-miR- MIMAT0005792 AAAAGCUGGGUUGAGAGGGCAA 610
320b-2 320b-2 320b
219 AAAAGCUGGGUUGAGAGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0005793 AAAAGCUGGGUUGAGAGGGU 611
320c-1 320c-1 320c
220 AAAAGCUGGGUUGAGAGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0005793 AAAAGCUGGGUUGAGAGGGU 612
320c-2 320c-2 320c
221 AAAAGCUGGGUUGAGAGGA hsa-mir- hsa-mir- hsa-miR- MIMAT0006764 AAAAGCUGGGUUGAGAGGA 613
320d-1 320d-1 320d
222 AAAAGCUGGGUUGAGAGGA hsa-mir- hsa-mir- hsa-miR- MIMAT0006764 AAAAGCUGGGUUGAGAGGA 614
320d-2 320d-2 320d
223 CACAUUACACGGUCGACCUCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000755 CACAUUACACGGUCGACCUCU 615
323 323 323-3p
224 CGCAUCCCCUAGGGCAUUGGUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000761 CGCAUCCCCUAGGGCAUUGGUGU 616
324 324 5324-p
225 CUGGCCCUCUCUGCCCUUCCGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000752 CUGGCCCUCUCUGCCCUUCCGU 617
328 328 328
226 GCAAAGCACACGGCCUGCAGAGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000751 GCAAAGCACACGGCCUGCAGAGA 618
330 330 330-3p
227 GCCCCUGGGCCUAUCCUAGAA hsa-mir- hsa-mir- hsa-miR- MIMAT0000760 GCCCCUGGGCCUAUCCUAGAA 619
331 331 331-3p
228 UCAAGAGCAAUAACGAAAAAUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000765 UCAAGAGCAAUAACGAAAAAUGU 620
335 335 335
229 UCCAGCAUCAGUGAUUUUGUUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000763 UCCAGCAUCAGUGAUUUUGUUG 621
338 338 338-3p
230 UGAGCGCCUCGACGACAGAGCCG hsa-mir- hsa-mir- hsa-miR- MIMAT0004702 UGAGCGCCUCGACGACAGAGCCG 622
339-3p 339 339-3p
231 GUGCAUUGUAGUUGCAUUGCA hsa-mir- hsa-mir- hsa-miR- MIMAT0000091 GUGCAUUGUAGUUGCAUUGCA 623
33a 33a 33a
232 GUGCAUUGCUGUUGCAUUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0003301 GUGCAUUGCUGUUGCAUUGC 624
33b 33b 33b
233 UUAUAAAGCAAUGAGACUGAUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004692 UUAUAAAGCAAUGAGACUGAUU 625
340 340 340
234 AGGGGUGCUAUCUGUGAUUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004694 AGGGGUGCUAUCUGUGAUUGA 626
342-5p 342 342-5p
235 GCUGACUCCUAGUCCAGGGCUC hsa-mir- hsa-mir- hsa-miR- MIMAT0000772 GCUGACUCCUAGUCCAGGGCUC 627
345 345 345
236 UGGCAGUGUCUUAGCUGGUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000255 UGGCAGUGUCUUAGCUGGUUGU 628
34a 34a 34a
237 AGGCAGUGUCAUUAGCUGAUUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000685 UAGGCAGUGUCAUUAGCUGAUUG 629
34b* 34b 34b*
238 AGGCAGUGUAGUUAGCUGAUUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0000686 AGGCAGUGUAGUUAGCUGAUUGC 630
34c-5p 34c 34c-5p
239 UUAUCAGAAUCUCCAGGGGUAC hsa-mir- hsa-mir- hsa-miR- MIMAT0000703 UUAUCAGAAUCUCCAGGGGUAC 631
361-5p 361 361-5p
240 AACACACCUAUUCAAGGAUUCA hsa-mir- hsa-mir- hsa-miR- MIMAT0004683 AACACACCUAUUCAAGGAUUCA 632
362-3p 362 362-3p
241 AAUUGCACGGUAUCCAUCUGUA hsa-mir- hsa-mir- hsa-miR- MIMAT0000707 AAUUGCACGGUAUCCAUCUGUA 633
363 363 363
242 UAAUGCCCCUAAAAAUCCUUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0000710 UAAUGCCCCUAAAAAUCCUUAU 634
365-2 365-2 365
243 AAUAAUACAUGGUUGAUCUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0000721 AAUAAUACAUGGUUGAUCUUU 635
369 369 369-3p
244 GCCUGCUGGGGUGGAACCUGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000722 GCCUGCUGGGGUGGAACCUGGU 636
370 370 370
245 ACUCAAACUGUGGGGGCACU hsa-mir- hsa-mir- hsa-miR- MIMAT0004687 ACUCAAACUGUGGGGGCACU 637
371 371 371-5p
246 UUAUAAUACAACCUGAUAAGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000727 UUAUAAUACAACCUGAUAAGUG 638
374a 374a 374a
247 AUAUAAUACAACCUGCUAAGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0004955 AUAUAAUACAACCUGCUAAGUG 639
374b 374b 374b
248 UUUGUUCGUUCGGCUCGCGUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000728 UUUGUUCGUUCGGCUCGCGUGA 640
375 375 375
249 AGAGGUUGCCCUUGGUGAAUUC hsa-mir- hsa-mir- hsa-miR- MIMAT0004689 AGAGGUUGCCCUUGGUGAAUUC 641
377* 377 377*
250 ACUGGACUUGGAGUCAGAAGG hsa-mir- hsa-mir- hsa-miR- MIMAT0000732 ACUGGACUUGGAGUCAGAAGG 642
378 378 378
251 UGGUAGACUAUGGAACGUAGG hsa-mir- hsa-mir- hsa-miR- MIMAT0000733 UGGUAGACUAUGGAACGUAGG 643
379 379 379
252 UAUACAAGGGCAAGCUCUCUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000736 UAUACAAGGGCAAGCUCUCUGU 644
381 381 381
253 GAAGUUGUUCGUGGUGGAUUCG hsa-mir- hsa-mir- hsa-miR- MIMAT0000737 GAAGUUGUUCGUGGUGGAUUCG 645
382 382 382
254 AGAUCAGAAGGUGAUUGUGGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000738 AGAUCAGAAGGUGAUUGUGGCU 646
383 383 383
255 CGAAUGUUGCUCGGUGAACCCC hsa-mir- hsa-mir- hsa-miR- MIMAT0001639 GAAUGUUGCUCGGUGAACCCCU 647
409-3p 409 409-3p
256 AAUAUAACACAGAUGGCCUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0002171 AAUAUAACACAGAUGGCCUGU 648
410 410 410
257 AUAGUAGACCGUAUAGCGUACG hsa-mir- hsa-mir- hsa-miR- MIMAT0003329 UAGUAGACCGUAUAGCGUACG 649
411 411 411
258 AUCAACAGACAUUAAUUGGGCGC hsa-mir- hsa-mir- hsa-miR- MIMAT0003339 AUCAACAGACAUUAAUUGGGCGC 650
421 421 421
259 UGAGGGGCAGAGAGCGAGACUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004748 UGAGGGGCAGAGAGCGAGACUUU 651
423 423 423-5p
260 CAGCAGCAAUUCAUGUUUUGAA hsa-mir- hsa-mir- hsa-miR- MIMAT0001341 CAGCAGCAAUUCAUGUUUUGAA 652
424 424 424
261 UAAUACUGUCUGGUAAAACCGU hsa-mir- hsa-mir- hsa-miR- MIMAT0001536 UAAUACUGUCUGGUAAAACCGU 653
429 429 429
262 UCUUGGAGUAGGUCAUUGGGUGG hsa-mir- hsa-mir- hsa-miR- MIMAT0002814 UCUUGGAGUAGGUCAUUGGGUGG 654
432 432 432
263 AUCAUGAUGGGCUCCUCGGUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0001627 AUCAUGAUGGGCUCCUCGGUGU 655
433 433 433
264 UUGCAUAUGUAGGAUGUCCCAU hsa-mir- hsa-mir- hsa-miR- MIMAT0001532 UUGCAUAUGUAGGAUGUCCCAU 656
448 448 448
265 UGGCAGUGUAUUGUUAGCUGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0001541 UGGCAGUGUAUUGUUAGCUGGU 657
449a 449a 449a
266 AGGCAGUGUAUUGUUAGCUGGC hsa-mir- hsa-mir- hsa-miR- MIMAT0003327 AGGCAGUGUAUUGUUAGCUGGC 658
449b 449b 449b
267 UUUUGCGAUGUGUUCCUAAUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0001545 UUUUGCGAUGUGUUCCUAAUAU 659
450a-1 450a-1 450a
268 UUUUGCGAUGUGUUCCUAAUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0001545 UUUUGCGAUGUGUUCCUAAUAU 660
450a-2 450a-2 450a
269 UUUUGCAAUAUGUUCCUGAAUA hsa-mir- hsa-mir- hsa-miR- MIMAT0004909 UUUUGCAAUAUGUUCCUGAAUA 661
450b-5p 450b 450b-5p
270 AACUGUUUGCAGAGGAAACUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0001635 AACUGUUUGCAGAGGAAACUGA 662
452 452 452
271 UAGUGCAAUAUUGCUUAUAGGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0003885 UAGUGCAAUAUUGCUUAUAGGGU 663
454 454 454
272 GCAGUCCAUGGGCAUAUACAC hsa-mir- hsa-mir- hsa-miR- MIMAT0004784 GCAGUCCAUGGGCAUAUACAC 664
455-3p 455 455-3p
273 AAGACGGGAGGAAAGAAGGGAG hsa-mir- hsa-mir- hsa-miR- MIMAT0004761 AAGACGGGAGGAAAGAAGGGAG 665
483-5p 483 483-5p
274 UCAGGCUCAGUCCCCUCCCGAU hsa-mir- hsa-mir- hsa-miR- MIMAT0002174 UCAGGCUCAGUCCCCUCCCGAU 666
484 484 484
275 AGAGGCUGGCCGUGAUGAAUUC hsa-mir- hsa-mir- hsa-miR- MIMAT0002175 AGAGGCUGGCCGUGAUGAAUUC 667
485-5p 485 485-5p
276 UCCUGUACUGAGCUGCCCCGAG hsa-mir- hsa-mir- hsa-miR- MIMAT0002177 UCCUGUACUGAGCUGCCCCGAG 668
486-5p 486 486-5p
277 AAUCGUACAGGGUCAUCCACUU hsa-mir- hsa-mir- hsa-miR- MIMAT0003180 AAUCGUACAGGGUCAUCCACUU 669
487b 487b 487b
278 CCCAGAUAAUGGCACUCUCAA hsa-mir- hsa-mir- hsa-miR- MIMAT0002804 CCCAGAUAAUGGCACUCUCAA 670
488* 488 488*
279 UUGUACAUGGUAGGCUUUCAUU hsa-mir- hsa-mir- hsa-miR- MIMAT0002813 UUGUACAUGGUAGGCUUUCAUU 671
493* 493 493*
280 UGAAACAUACACGGGAAACCUC hsa-mir- hsa-mir- hsa-miR- MIMAT0002816 UGAAACAUACACGGGAAACCUC 672
494 494 494
281 AAACAAACAUGGUGCACUUCUU hsa-mir- hsa-mir- hsa-miR- MIMAT0002817 AAACAAACAUGGUGCACUUCUU 673
495 495 495
282 CAGCAGCACACUGUGGUUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0002820 CAGCAGCACACUGUGGUUUGU 674
497 497 497
283 UUAAGACUUGCAGUGAUGUUU hsa-mir- hsa-mir- hsa-miR- MIMAT0002870 UUAAGACUUGCAGUGAUGUUU 675
499-5p 499 499-5p
284 AUGCACCUGGGCAAGGAUUCUG hsa-mir- hsa-mir- hsa-miR- MIMAT0002871 AUGCACCUGGGCAAGGAUUCUG 676
500* 500 500*
285 AAUGCACCCGGGCAAGGAUUCU hsa-mir- hsa-mir- hsa-miR- MIMAT0004774 AAUGCACCCGGGCAAGGAUUCU 677
501-3p 501 501-3p
286 AAUGCACCUGGGCAAGGAUUCA hsa-mir- hsa-mir- hsa-miR- MIMAT0004775 AAUGCACCUGGGCAAGGAUUCA 678
502-3p 502 502-3p
287 UAGCAGCGGGAACAGUUCUGCAG hsa-mir- hsa-mir- hsa-miR- MIMAT0002874 UAGCAGCGGGAACAGUUCUGCAG 679
503 503 503
288 AGACCCUGGUCUGCACUCUAUC hsa-mir- hsa-mir- hsa-miR- MIMAT0002875 AGACCCUGGUCUGCACUCUAUC 680
504 504 504
289 GGGAGCCAGGAAGUAUUGAUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0004776 GGGAGCCAGGAAGUAUUGAUGU 681
505* 505 505*
290 UGAUUGUAGCCUUUUGGAGUAGA hsa-mir- hsa-mir- hsa-miR- MIMAT0002880 UGAUUGUAGCCUUUUGGAGUAGA 682
508-3p 508 508-3p
291 UACUGCAGACGUGGCAAUCAUG hsa-mir- hsa-mir- hsa-miR- MIMAT0004975 UACUGCAGACGUGGCAAUCAUG 683
509-3-5p 509-3 509-3-5p
292 UUCACAGGGAGGUGUCAU hsa-mir- hsa-mir- hsa-miR- MIMAT0002877 UUCACAGGGAGGUGUCAU 684
513a-1 513a-1 513a-5p
293 UUCACAGGGAGGUGUCAU hsa-mir- hsa-mir- hsa-miR- MIMAT0002877 UUCACAGGGAGGUGUCAU 685
513a-2 513a-2 513a-5p
294 UUCACAAGGAGGUGUCAUUUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0005788 UUCACAAGGAGGUGUCAUUUAU 686
513b 513b 513b
295 UUCUCAAGGAGGUGUCGUUUAU hsa-mir- hsa-mir- hsa-miR- MIMAT0005789 UUCUCAAGGAGGUGUCGUUUAU 687
513c 513c 513c
296 CAUGCCUUGAGUGUAGGACCGU hsa-mir- hsa-mir- hsa-miR- MIMAT0002888 CAUGCCUUGAGUGUAGGACCGU 688
532 532 532-5p
297 UGUGACAGAUUGAUAACUGAAA hsa-mir- hsa-mir- hsa-miR- MIMAT0003389 UGUGACAGAUUGAUAACUGAAA 689
542-3p 542 542-3p
298 AAACAUUCGCGGUGCACUUCUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004954 AAACAUUCGCGGUGCACUUCUU 690
543 543 543
299 CAAAACUGGCAAUUACUUUUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0003251 CAAAACUGGCAAUUACUUUUGC 691
548a-1 548a-1 548a-3p
300 CAAAACUGGCAAUUACUUUUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0003251 CAAAACUGGCAAUUACUUUUGC 692
548a-2 548a-2 548a-3p
301 CAAAACUGGCAAUUACUUUUGC hsa-mir- hsa-mir- hsa-miR- MIMAT0003251 CAAAACUGGCAAUUACUUUUGC 693
548a-3 548a-3 548a-3p
302 CAAGAACCUCAGUUGCUUUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0003254 CAAGAACCUCAGUUGCUUUUGU 694
548b-3p 548b 548b-3p
303 AAAAACUGAGACUACUUUUGCA hsa-mir- hsa-mir- hsa-miR- MIMAT0005874 AAAAACUGAGACUACUUUUGCA 695
548e 548e 548e
304 AAAAGUAAUCGCGGUUUUUGUC hsa-mir- hsa-mir- hsa-miR- MIMAT0005928 AAAAGUAAUCGCGGUUUUUGUC 696
548h-1 548h-1 548h
305 AAAAGUAAUCGCGGUUUUUGUC hsa-mir- hsa-mir- hsa-miR- MIMAT0005928 AAAAGUAAUCGCGGUUUUUGUC 697
548h-2 548h-2 548h
306 AAAAGUAAUCGCGGUUUUUGUC hsa-mir- hsa-mir- hsa-miR- MIMAT0005928 AAAAGUAAUCGCGGUUUUUGUC 698
548h-3 548h-3 548h
307 AAAAGUAAUCGCGGUUUUUGUC hsa-mir- hsa-mir- hsa-miR- MIMAT0005928 AAAAGUAAUCGCGGUUUUUGUC 699
548h-4 548h-4 548h
308 AAAAGUAAUUGCGGUCUUUGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0005875 AAAAGUAAUUGCGGUCUUUGGU 700
548j 548j 548j
309 AAAAGUACUUGCGGAUUUUGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0005882 AAAAGUACUUGCGGAUUUUGCU 701
548k 548k 548k
310 AAAAGUAUUUGCGGGUUUUGUC hsa-mir- hsa-mir- hsa-miR- MIMAT0005889 AAAAGUAUUUGCGGGUUUUGUC 702
548l 548l 548l
311 CAAAAGUAAUUGUGGAUUUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0005916 CAAAAGUAAUUGUGGAUUUUGU 703
548n 548n 548n
312 AGUGCCUGAGGGAGUAAGAGCCC hsa-mir- hsa-mir- hsa-miR- MIMAT0004800 AGUGCCUGAGGGAGUAAGAGCCC 704
550-1 550-1 550
313 AGUGCCUGAGGGAGUAAGAGCCC hsa-mir- hsa-mir- hsa-miR- MIMAT0004800 AGUGCCUGAGGGAGUAAGAGCCC 705
550-2 550-2 550
314 GCGACCCAUACUUGGUUUCAG hsa-mir- hsa-mir- hsa-miR- MIMAT0003233 GCGACCCAUACUUGGUUUCAG 706
551b 551b 551b
315 CACGCUCAUGCACACACCCACA hsa-mir- hsa-mir- hsa-miR- MIMAT0003239 CACGCUCAUGCACACACCCACA 707
574-3p 574 574-3p
316 AAGAUGUGGAAAAAUUGGAAUC hsa-mir- hsa-mir- hsa-miR- MIMAT0004796 AAGAUGUGGAAAAAUUGGAAUC 708
576-3p 576 576-3p
317 GUAGAUAAAAUAUUGGUACCUG hsa-mir- hsa-mir- hsa-miR- MIMAT0003242 UAGAUAAAAUAUUGGUACCUG 709
577 577 577
318 UAACUGGUUGAACAACUGAACC hsa-mir- hsa-mir- hsa-miR- MIMAT0004797 UAACUGGUUGAACAACUGAACC 710
582-3p 582 582-3p
319 UUACAGUUGUUCAACCAGUUACU hsa-mir- hsa-mir- hsa-miR- MIMAT0003247 UUACAGUUGUUCAACCAGUUACU 711
582-5p 582 582-5p
320 UUAUGGUUUGCCUGGGACUGAG hsa-mir- hsa-mir- hsa-miR- MIMAT0003249 UUAUGGUUUGCCUGGGACUGAG 712
584 584 584
321 UGAGAACCACGUCUGCUCUGAG hsa-mir- hsa-mir- hsa-miR- MIMAT0004799 UGAGAACCACGUCUGCUCUGAG 713
589 589 589
322 UAAUUUUAUGUAUAAGCUAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0004801 UAAUUUUAUGUAUAAGCUAGU 714
590-3p 590 590-3p
323 UACGUCAUCGUUGUCAUCGUCA hsa-mir- hsa-mir- hsa-miR- MIMAT0003266 UACGUCAUCGUUGUCAUCGUCA 715
598 598 598
324 UCCGAGCCUGGGUCUCCCUCUU hsa-mir- hsa-mir- hsa-miR- MIMAT0003283 UCCGAGCCUGGGUCUCCCUCUU 716
615-3p 615 615-3p
325 AAGUCAUUGGAGGGUUUGAGCA hsa-mir- hsa-mir- hsa-miR- MIMAT0004805 AGUCAUUGGAGGGUUUGAGCAG 717
616 616 616
326 AAACUCUACUUGUCCUUCUGAGU hsa-mir- hsa-mir- hsa-miR- MIMAT0003287 AAACUCUACUUGUCCUUCUGAGU 718
618 618 618
327 AGGGGGAAAGUUCUAUAGUCC hsa-mir- hsa-mir- hsa-miR- MIMAT0003294 AGGGGGAAAGUUCUAUAGUCC 719
625 625 625
328 AUGCUGACAUAUUUACUAGAGG hsa-mir- hsa-mir- hsa-miR- MIMAT0004809 AUGCUGACAUAUUUACUAGAGG 720
628-5p 628 628-5p
329 UGGGUUUACGUUGGGAGAACU hsa-mir- hsa-mir- hsa-miR- MIMAT0004810 UGGGUUUACGUUGGGAGAACU 721
629 629 629
330 AAAGACAUAGGAUAGAGUCACCUC hsa-mir- hsa-mir- hsa-miR- MIMAT0003311 AAAGACAUAGGAUAGAGUCACCUC 722
641 641 641
331 ACACUUGUAUGCUAGCUCAGGU hsa-mir- hsa-mir- hsa-miR- MIMAT0003313 ACUUGUAUGCUAGCUCAGGUAG 723
643 643 643
332 UUUAGGAUAAGCUUGACUUUUG hsa-mir- hsa-mir- hsa-miR- MIMAT0003321 UUUAGGAUAAGCUUGACUUUUG 724
651 651 651
333 AAUGGCGCCACUAGGGUUGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0003322 AAUGGCGCCACUAGGGUUGUG 725
652 652 652
334 UAUGUCUGCUGACCAUCACCUU hsa-mir- hsa-mir- hsa-miR- MIMAT0004814 UAUGUCUGCUGACCAUCACCUU 726
654-3p 654 654-3p
335 UACCCAUUGCAUAUCGGAGUUG hsa-mir- hsa-mir- hsa-miR- MIMAT0003338 UACCCAUUGCAUAUCGGAGUUG 727
660 660 660
336 UCCGGUUCUCAGGGCUCCACC hsa-mir- hsa-mir- hsa-miR- MIMAT0004819 UCCGGUUCUCAGGGCUCCACC 728
671-3p 671 671-3p
337 AAGGAGCUUACAAUCUAGCUGGG hsa-mir- hsa-mir- hsa-miR- MIMAT0004926 AAGGAGCUUACAAUCUAGCUGGG 729
708 708 708
338 UGGAAGACUAGUGAUUUUGUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000252 UGGAAGACUAGUGAUUUUGUUGU 730
7-1 7-1 7
339 UGGAAGACUAGUGAUUUUGUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000252 UGGAAGACUAGUGAUUUUGUUGU 731
7-2 7-2 7
340 UGGAAGACUAGUGAUUUUGUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000252 UGGAAGACUAGUGAUUUUGUUGU 732
7-3 7-3 7
341 CGGCUCUGGGUCUGUGGGGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004957 CGGCUCUGGGUCUGUGGGGA 733
760 760 760
342 UGCACCAUGGUUGUCUGAGCAUG hsa-mir- hsa-mir- hsa-miR- MIMAT0003882 UGCACCAUGGUUGUCUGAGCAUG 734
767-5p 767 767-5p
343 UGAGACCUCUGGGUUCUGAGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0003886 UGAGACCUCUGGGUUCUGAGCU 735
769-5p 769 769-5p
344 GCAGGAACUUGUGAGUCUCCU hsa-mir- hsa-mir- hsa-miR- MIMAT0004953 GCAGGAACUUGUGAGUCUCCU 736
873 873 873
345 CUGCCCUGGCCCGAGGGACCGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004911 CUGCCCUGGCCCGAGGGACCGA 737
874 874 874
346 GUAGAGGAGAUGGCGCAGGG hsa-mir- hsa-mir- hsa-miR- MIMAT0004949 GUAGAGGAGAUGGCGCAGGG 738
877 877 877
347 GUGAACGGGCGCCAUCCCGAGG hsa-mir- hsa-mir- hsa-miR- MIMAT0004951 GUGAACGGGCGCCAUCCCGAGG 739
887 887 887
348 UUAAUAUCGGACAACCAUUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0004921 UUAAUAUCGGACAACCAUUGU 740
889 889 889
349 UGCAACGAACCUGAGCCACUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0004902 UGCAACGAACCUGAGCCACUGA 741
891a 891a 891a
350 CACUGUGUCCUUUCUGCGUAG hsa-mir- hsa-mir- hsa-miR- MIMAT0004907 CACUGUGUCCUUUCUGCGUAG 742
892a 892a 892a
351 UCUUUGGUUAUCUAGCUGUAUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000441 UCUUUGGUUAUCUAGCUGUAUGA 743
9-1 9-1 9
352 UCUUUGGUUAUCUAGCUGUAUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000441 UCUUUGGUUAUCUAGCUGUAUGA 744
9-2 9-2 9
353 UAUUGCACUUGUCCCGGCCUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000092 UAUUGCACUUGUCCCGGCCUGU 745
92a-1 92a-1 92a
354 UAUUGCACUUGUCCCGGCCUGU hsa-mir- hsa-mir- hsa-miR- MIMAT0000092 UAUUGCACUUGUCCCGGCCUGU 746
92a-2 92a-2 92a
355 UAUUGCACUCGUCCCGGCCUCC hsa-mir- hsa-mir- hsa-miR- MIMAT0003218 UAUUGCACUCGUCCCGGCCUCC 747
92b 92b 92b
356 CAAAGUGCUGUUCGUGCAGGUAG hsa-mir- hsa-mir- hsa-miR- MIMAT0000093 CAAAGUGCUGUUCGUGCAGGUAG 748
93 93 93
357 UCUUUGGUUAUCUAGCUGUAUGA hsa-mir- hsa-mir- hsa-miR- MIMAT0000441 UCUUUGGUUAUCUAGCUGUAUGA 749
9-3 9-3 9
358 AAGGCAGGGCCCCCGCUCCCC hsa-mir- hsa-mir- hsa-miR- MIMAT0004983 AAGGCAGGGCCCCCGCUCCCC 750
940 940 940
359 UUCUCUGUUUUGGCCAUGUGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0004985 UCUUCUCUGUUUUGGCCAUGUG 751
942 942 942
360 AAAUUAUUGUACAUCGGAUGAG hsa-mir- hsa-mir- hsa-miR- MIMAT0004987 AAAUUAUUGUACAUCGGAUGAG 752
944 944 944
361 UUCAACGGGUAUUUAUUGAGCA hsa-mir- hsa-mir- hsa-miR- MIMAT0000094 UUCAACGGGUAUUUAUUGAGCA 753
95 95 95
362 UUUGGCACUAGCACAUUUUUGCU hsa-mir- hsa-mir- hsa-miR- MIMAT0000095 UUUGGCACUAGCACAUUUUUGCU 754
96 96 96
363 UGAGGUAGUAAGUUGUAUUGUU hsa-mir- hsa-mir- hsa-miR- MIMAT0000096 UGAGGUAGUAAGUUGUAUUGUU 755
98 98 98
364 AACCCGUAGAUCCGAUCUUGUG hsa-mir- hsa-mir- hsa-miR- MIMAT0000097 AACCCGUAGAUCCGAUCUUGUG 756
99a 99a 99a
365 CACCCGUAGAACCGACCUUGCG hsa-mir- hsa-mir- hsa-miR- MIMAT0000689 CACCCGUAGAACCGACCUUGCG 757
99b 99b 99b
366 AACAUAGAGGAAAUUCCACGU hsa-mir- hsa-mir- hsa-mir- AACAUAGAGGAAAUUCCACGU 758
376c 376c 376c
367 AUCAUAGAGGAAAAUCCAUGUU hsa-mir- hsa-mir- hsa-mir- AUCAUAGAGGAAAAUCCAUGUU 759
376b 376b 376b
368 UGGUGGGCCGCAGAACAUGUGC hsa-mir- hsa-mir- hsa-mir- UGGUGGGCCGCAGAACAUGUGC 760
654 654 654
369 AUCAUAGAGGAAAAUCCACGU hsa-mir- hsa-mir- hsa-mir- AUCAUAGAGGAAAAUCCACGU 761
376a-2 376a-2 376a-2
370 AUCAUAGAGGAAAAUCCACGU hsa-mir- hsa-mir- hsa-mir- AUCAUAGAGGAAAAUCCACGU 762
376a-1 376a-1 376a-1
371 AAAACCGUCUAGUUACAGUUGU hsa-mir-
1537
372 AUAUACAGGGGGAGACUCUCAU hsa-mir-
1185-2
373 CCUAGAAUGGGGAUUGUGGG hsa-mir-
1301*
374 AGCGAGACCUCAACUCUACAAU hsa-mir-
1303
375 UCGACCGGACCUCGACCGGCUC hsa-mir-
1307*
376 AUGUAGGGAUGGAAGCCAUGAA hsa-mir-
135a-2
377 CUCACUGAACAAUGAAUGCAA hsa-mir-
181b-1*
378 CGGGUAGAGAGGGCAGUGGGAG hsa-mir-
197*
379 GCUGGGAAGGCAAAGGGACGU hsa-mir-
204*
380 ACCUUGGCUCUAGACUGCUUAC hsa-mir-
212*
381 GCUCUGACUUUAUUGCACUACU hsa-mir-
301a
382 AGGGACUUUUGGGGGCAGAUGU hsa-mir-
365-1
383 UUGGGGACAUUUUGCAUUCAU hsa-mir-
450a-2*
384 AAUGUGUAGCAAAAGACAGA hsa-mir-
511-1-3p
385 AAUGUGUAGCAAAAGACAGA hsa-mir-
511-2-3p
386 AUCAUACAAGGACAAUUUCUUU hsa-mir-
539
387 AAAGGUAAUUGCAGUUUUUCCC hsa-mir-
570
388 UCGCGGUUUGUGCCAGAUGACG hsa-mir-
579
389 AGAAGGCACUAUGAGAUUUAGA hsa-mir-
605
390 AGACACAUUUGGAGAGGGAACC hsa-mir-
642
391 AGGACCUUCCCUGAACCAAGGA hsa-mir-
659
392 AUAUACAGGGGGAGACUCUUAU hsa-mir-
1185-1
TABLE 32
Novel miRNAs identified by deep sequencing analysis.
SEQ Temporary SEQ
ID Mature Assigned miRBase ID
NO. Sequence Captured miRNA ID ID NO. miRNA Precursor Sequence
763 CCAAAACUGCAGUUACUUUUG has-mir-548o- pending 1057 UggUgcaaaagUaaUUgcggUUUUUgccaUUaaaagUaaUgc
2 ggCCAAAACUGCAGUUACUUUUGcaccc
764 GAGCCUGGAAGCUGGAGCCUGC hsa-mir-1254- hsa-mir- 1058 cUGAGCCUGGAAGCUGGAGCCUGCagUgagcUaUgaUca
2 1254-2 UgUcccUgUacUcUagccUgggca
765 CGGGCGUGGUGGUGGGGGUG hsa-mir-1268b hsa-mir- 1059 accCGGGCGUGGUGGUGGGGGUGggUgccUgUaaUUcca
1268b gcUagUUggga
766 UCGAGGAGCUCACAGUCUAGA hsa-miR-151- hsa-mir- 1060 agUcUcUcUUcagggcUcccgagacacagaaacagacaccUgccc
5p-2 151b UCGAGGAGCUCACAGUCUAGAca
767 UAAGGUGCAUCUAGUGCAGUU hsa-mir-18b- hsa-mir- 1061 UcUUgUgUUAAGGUGCAUCUAGUGCAGUUagUgaagcag
2 18b cUUagaaUcUacUgcccUaaaUgccccUUcUggcacaggc
768 GAGGGUUGGGUGGAGGC hsa-miR-296- hsa-miR- 1062 cUgccUccaccccgccUggccUgacccagccagggcUcUagGAGG
2 296-2 GUUGGGUGGAGGCaa
769 AUCAUAGAGGAAAAUCCAUGUU hsa-mir-376b- hsa-mir- 1063 UaaaacgUggaUaUUccUUcUaUgUUUacgUgaUUccUggU
2 376b UaAUCAUAGAGGAAAAUCCAUGUUUUc
770 AACAUAGAGGAAAUUCCACGU hsa-mir-376c- hsa-mir- 1064 UaaaaggUggaUaUUccUUcUaUgUUUaUgUUaUUUaUgg
2 376c UUaAACAUAGAGGAAAUUCCACGUUUUc
771 ACUGGACUUGGAGUCAGAAA hsa-mir-378b- hsa-mir- 1065 acUgUUUcUgUccUUgUUcUUgUUgUUaUUACUGGACU
1 378d-1 UGGAGUCAGAAAcagg
772 ACUGGACUUGGAGUCAGAAA hsa-mir-378b- hsa-mir- 1066 aggagagaacACUGGACUUGGAGUCAGAAAacUUUcaUcc
2 378d-2 aagUcaUUcccUgcUcUaagUcccaUUUcUgUUcca
773 ACUGGACUUGGAGUCAGGA hsa-miR-378c hsa-mir- 1067 cUgacUccagUgUccaggccaggggcagacagUggacagagaacag
378e UgcccaagaccACUGGACUUGGAGUCAGGAcaU
774 AGGCAGUGUAUUGCUAGCGGCU hsa-mir-449c hsa-mir- 1068 UcaggUAGGCAGUGUAUUGCUAGCGGCUgUUaaUgaUU
449c UUaacagUUgcUagUUgcacUccUcUcUgU
775 UGCACCCAGGCAAGGAUUCUGC hsa-mir-500- hsa-mir- 1069 gUUcccccUcUcUaaUccUUgcUaccUgggUgagagUgcUUUc
2 500b UgaaUgcagUGCACCCAGGCAAGGAUUCUGCaagggggag
U
776 UAAUCCUUGCUACCUGGGUGAG hsa-mir-500- has-mir- 1070 gUUcccccUcUcUAAUCCUUGCUACCUGGGUGAGagUgc
2* 500b UUUcUgaaUgcagUgcacccaggcaaggaUUcUgcaagggggag
U
777 AAAAGUAAUCGCGGUUUUUGUC hsa-mir-548h- hsa-mir- 1071 acAAAAGUAAUCGCGGUUUUUGUCaUUacUUUUaacUg
5 548h-5 UaaaaaccacggUUgcUUUUgc
778 AAAAGUAAUUGUGGAUUUUGCU hsa-mir-548r- hsa-mir- 1072 aUgUUggUgcAAAAGUAAUUGUGGAUUUUGCUaUUacU
1 548ab UgUaUUUaUUUgUaaUgcaaaacccgcaaUUagUUUUgcacc
aacc
779 AAAAGUAAUUGUGGAUUUUGCU hsa-mir-548r- has-mir- 1073 UgUUggUgcAAAAGUAAUUGUGGAUUUUGCUaUUacU
2 548ab UgUaUUUaUUUgUaaUgcaaaacccgcaaUUagUUUUgcacc
aacc
780 UGUCUUACUCCCUCAGGCACAU hsa-miR-550- pending 1074 cUggUgcagUgccUgagggagUaagagUccUgUUgUUgUaaga
3* UagUGUCUUACUCCCUCAGGCACAUcUccaa
781 GGUGGGCUUCCCGGAGGG hsa-mir-2221 hsa-mir- 1075 gaaaacaaccaGGUGGGCUUCCCGGAGGGcggaacacccagc
4417 cccagcaUccagggcUcaccUaccacgUUUg
782 GAAGCGGUGGCUGGGCUG hsa-mir-2222 pending 1076 gaggcggcccUagcgccaUUUUgUgggagcGAAGCGGUGGCU
GGGCUGcgcUUg
783 CACUGCAGGACUCAGCAG hsa-mir-2223 hsa-mir- 1077 UggUUUUUgcUcUgagUgaccgUggUggUUgUgggagUCAC
4418 UGCAGGACUCAGCAGgaaUUc
784 UGAGGGAGGAGACUGCA hsa-mir-2224 hsa-mir- 1078 UggUggUgUgUgccUgUagUcUUagcUacUcgggaggcUGAG
4419a GGAGGAGACUGCAgUgagUggaggUcacgccacUg
785 ACUGGACUUGGAGCCAGAAG hsa-mir-2225 hsa-mir- 1079 gUcaggUccUggacUcccaUagUUUUcaggcUgcUaaacaacag
378f aacgagcACUGGACUUGGAGCCAGAAGUcUUggg
786 GUCACUGAUGUCUGUAGCUGAG hsa-mir-2226 hsa-mir- 1080 cUcUUggUaUgaacaUcUgUgUgUUcaUgUcUcUcUgUgca
4420 caggggacgagaGUCACUGAUGUCUGUAGCUGAGac
787 GAUGAGGAUGGAUAGCAAGGAA hsa-mir-2227 hsa-mir- 1081 cUggccUcUgUgccUggaUacUUUaUacgUgUaaUUgUGAU
3605 GAGGAUGGAUAGCAAGGAAgccgc
788 ACCUGUCUGUGGAAAGGAGCUA hsa-mir-2228 hsa-mir- 1082 cUgggUcUccUUUcUgcUgagagUUgaacacUUgUUgggaca
4421 ACCUGUCUGUGGAAAGGAGCUAccUac
789 AAAAGCAUCAGGAAGUACCCA hsa-mir-2229 hsa-mir- 1083 agUUcUUcUgcagacAAAAGCAUCAGGAAGUACCCAccaU
4422 gUaccagUgggcccUUcUUgaUgcUcUUgaUUgcagaggagcc
790 UGCCUGGAACAUAGUAGGGACU hsa-mir-2230- hsa-mir- 1084 UccUUUaUUgagUcccUacUaUgUUccaggcaccUacgaUacc
1 3116-2 cagUGCCUGGAACAUAGUAGGGACUcaaUaaagU
791 UGCCUGGAACAUAGUAGGGACU hsa-mir-2230- hsa-mir- 1085 ccUUUaUUgagUcccUacUaUgUUccaggcaccUacgaUaccc
2 3116-1 agUGCCUGGAACAUAGUAGGGACUcaaUaaagU
792 UGGAUUAAAAACAAUGGAGG hsa-mir-2231 pending 1086 cgccUccaUgUUUcagcaUcUaUgUcaUgggcUUggUccUgga
gUGGAUUAAAAACAAUGGAGGU
793 AUAGGCACCAAAAAGCAACAA hsa-mir-2232 hsa-mir- 1087 aUcaUgUacUgcagUUgccUUUUUgUUcccaUgcUgUUUaa
4423 gccUagcAUAGGCACCAAAAAGCAACAAcagUaUgUgaa
794 ACUGGGCUUGGAGUCAGAAG hsa-mir-2233 hsa-mir- 1088 cACUGGGCUUGGAGUCAGAAGaccUggcUccagcccagcUc
378g gUaUUaggUUggUgcaaaagUUaUUgUggUUUUUgcUaUU
795 CAAAAACCGGCAAUUACUUUUG hsa-mir-2234 hsa-mir- 1089 UUUUUUUaaUggCAAAAACCGGCAAUUACUUUUGcacU
548ac aaccUagUag
796 GUCAAAUGAAGGGCUGAUCACG hsa-mir-2235 pending 1090 aaagUGUCAAAUGAAGGGCUGAUCACGaaaUagcgcaUU
agcUcUUUUUUUgaaaacUUg
797 AAAGGGAGGAUUUGCUUAGAAGG hsa-mir-2236 pending 1091 gacUggcUacgUagUUcgggcaaaUccUccaaaagggAAAGGG
AUGG AGGAUUUGCUUAGAAGGAUGGcgcUcc
798 AGAGUUAACUCAAAAUGGACUA hsa-mir-2237 hsa-mir- 1092 cUUacaUcacacacAGAGUUAACUCAAAAUGGACUAaUU
4424 UUUccacUagUUagUccaUUUcaagUUaacUcUgUgUgUga
UgUagU
799 UGUUGGGAUUCAGCAGGACCAU hsa-mir-2238 hsa-mir- 1093 gUgcUUUacaUgaaUggUcccaUUgaaUcccaacagcUUUgcg
4425 aagUgUUGUUGGGAUUCAGCAGGACCAUUcgUgUaaag
Uaa
800 UAAAUAGAGUAGGCAAAGGACA hsa-mir-2239 hsa-mir- 1094 UaaaUggUUaUgUccUUUgccUaUUcUaUUUaagacacccU
3121 gUaccUUAAAUAGAGUAGGCAAAGGACAgaaacaUUUU
801 AGAGUCGAGAGUGGGAGAAGAG hsa-mir-2240 pending 1095 gcAGAGUCGAGAGUGGGAGAAGAGcggagcgUgUgagcag
UacUgcggccUccUcUccUcUccUaaccUcgcUcUc
802 GAAGAUGGACGUACUUU hsa-mir-2241 hsa-mir- 1096 agUUGAAGAUGGACGUACUUUgUcUgacUacaaUaUUca
4426 aaaggagUcUacUcUUcaUcUUg
803 UCUGAAUAGAGUCUGAAGAGU hsa-mir-2242 hsa-mir- 1097 gaagccUcUUggggcUUaUUUagacaaUggUUUcaUcaUUUc
4427 gUCUGAAUAGAGUCUGAAGAGUcUUU
804 CAAGGAGACGGGAACAUGGAGC hsa-mir-2243 hsa-mir- 1098 UUggcaggUgccaUgUUgccUgcUccUUacUgUacacgUggcU
4428 ggCAAGGAGACGGGAACAUGGAGCcgccaU
805 AAGAGGAAGAAAUGGCUGGUUC hsa-mir-2244 hsa-mir- 1099 aggaagAAGAGGAAGAAAUGGCUGGUUCUcaggUgaaUg
3916 UgUcUgggUUcaggggaUgUgUcUccUcUUUUcU
806 UUCGCGGGCGAAGGCAAAGUC hsa-mir-2245 hsa-mir- 1100 ggcgggcUUCGCGGGCGAAGGCAAAGUCgaUUUccaaaag
3124 UgacUUUccUcacUcccgUgaagUcggcg
807 AAAAGCUGGGCUGAGAGGCG hsa-mir-2246 hsa-mir- 1101 agggagAAAAGCUGGGCUGAGAGGCGacUggUgUcUaaU
4429 UUgUUUgUcUcUccaacUcagacUgccUggccca
808 AUGGCCAAAACUGCAGUUAUUU hsa-mir-2247 hsa-mir- 1102 cUgcaaaaaUaaUUgcagUUUUUgccaUUaUUUUUaaUaa
548s UUaUaaUaAUGGCCAAAACUGCAGUUAUUUUUgcac
809 UAGUGGAUGAUGCACUCUGUGC hsa-mir-2248 hsa-mir- 1103 cUacUUccagUAGUGGAUGAUGCACUCUGUGCagggccaa
3681 cUgUgcacacagUgcUUcaUccacUacUggaagUgU
810 AGGCUGGAGUGAGCGGAG hsa-mir-2249 hsa-mir- 1104 gUgAGGCUGGAGUGAGCGGAGaUcgUaccacUgcacUcca
4430 accUggUga
811 GAAAACGACAAUGACUUUUGCA hsa-mir-2250 hsa-mir- 1105 cUgUUaggUUggUgcaaaagUaaUUgUggUUUUUgaaagUa
548ad acUUggcGAAAACGACAAUGACUUUUGCAccaaUcUaaUa
c
812 GCGACUCUGAAAACUAGAAGGU hsa-mir-2251 hsa-mir- 1106 UggUUUGCGACUCUGAAAACUAGAAGGUUUaUgacUgg
4431 gcaUUUcUcacccaaUgcccaaUaUUgaacUUUcUagUUgUc
agagUcaUUaaccc
813 AGAAGGCUGGAGCGCGGCGGU hsa-mir-2252 pending 1107 gcacUgcggUUcUgaggccgUUacUccggcUUcUccaUagaggg
cggAGAAGGCUGGAGCGCGGCGGUga
814 AAAGACUCUGCAAGAUGCCU hsa-mir-2253 hsa-mir- 1108 gcaUcUUgcagagccgUUccaaUgcgacaccUcUagagUgUcaU
4432 ccccUagaaUgUcaccUUggAAAGACUCUGCAAGAUGCCU
815 ACAGGAGUGGGGGUGGGACAU hsa-mir-2254 hsa-mir- 1109 caUccUccUUacgUcccaccccccacUccUgUUUcUggUgaaaU
4433 aUUcaaACAGGAGUGGGGGUGGGACAUaaggaggaUa
816 AGGAGAAGUAAAGUAGAA hsa-mir-2255 hsa-mir- 1110 UcacUUUAGGAGAAGUAAAGUAGAAcUUUggUUUUcaa
4434 cUUUUccUacagUgU
817 AUGGCCAGAGCUCACACAGAGG hsa-mir-2256 hsa-mir- 1111 aggcagcaaAUGGCCAGAGCUCACACAGAGGgaUgagUgca
4435-1 cUUcaccUgcagUgUgacUcagcaggccaacagaUgcUa
818 GCAGGACAGGCAGAAGUGGAU hsa-mir-2257 hsa-mir- 1112 gccUcacUUUUccacUUaUgccUgcccUgccccUcgaaUcUgcU
4436 ccacgaUUUggGCAGGACAGGCAGAAGUGGAUaagUgagg
a
819 AUCAGGGCUUGUGGAAUGGGAA hsa-mir-2258 hsa-mir- 1113 ggcccAUCAGGGCUUGUGGAAUGGGAAggagaagggacgc
3127 UUccccUUcUgcaggccUgcUgggUg
820 AUGGCCAGAGCUCACACAGAGG hsa-mir-2259 hsa-mir- 1114 gcaaAUGGCCAGAGCUCACACAGAGGgaUgagUgcacUUc
4435-2 accUgcagUgUgacUcagcaggccaacagaUgcU
821 UGAGGAUAUGGCAGGGAAGGGG hsa-mir-2260 hsa-mir- 1115 acgUggUGAGGAUAUGGCAGGGAAGGGGagUUUcccUc
3679 UaUUcccUUccccccagUaaUcUUcaUcaUgc
822 AGGAGGUUGGUGUGGAUU hsa-mir-2261 pending 1116 UaUgAGGAGGUUGGUGUGGAUUcUgUUgaagaaaaagaa
ggggaacacUaaUUUUccaUU
823 UUGGAAGACAUGGAGCAUGAGG hsa-mir-2262 pending 1117 UUUUGGAAGACAUGGAGCAUGAGGUaagUgccUagaUcc
UcaaaccacUUgccUccaccUaUgcUUccaggU
824 UCUGGCAAGUAAAAAACUCUCA hsa-mir-2263 hsa-mir- 1118 cUUccUCUGGCAAGUAAAAAACUCUCAUUUUccUUaaaa
3128 aaUgagagUUUUUUacUUgcaaUaggaaa
825 UGGGCUCAGGGUACAAAGGUU hsa-mir-2264 hsa-mir- 1119 acUUUgUgcaUUgggUccacaaggaggggaUgacccUUgUGG
4437 GCUCAGGGUACAAAGGUU
826 CAAAAACUGCAAUUACUUUCA hsa-mir-2265- hsa-mir- 1120 gcagUUUUUgccaUUaagUUgcggUUUUUgccaUUaUaaUg
1 548ae-1 gCAAAAACUGCAAUUACUUUCAcaccUgc
827 CAAAAACUGCAAUUACUUUCA hsa-mir-2265- hsa-mir- 1121 UgUgcaaaagUaaUUgUggUUUUUgUcaUUUaaaagUaaUg
2 548ae-2 gCAAAAACUGCAAUUACUUUCAcacc
828 GCUGCACCGGAGACUGGGUAA hsa-mir-2266- hsa-mir- 1122 acUUgUcaUgUcUUacccagUcUccggUgcagccUgUUgUcaa
1 3130-1 gGCUGCACCGGAGACUGGGUAAgacaUgacaagc
829 GCUGCACCGGAGACUGGGUAA hsa-mir-2266- hsa-mir- 1123 UgUcUUacccagUcUccggUgcagccUUgacaacagGCUGCAC
2 3130-2 CGGAGACUGGGUAAgacaUgacaagUU
830 AUCCCCAGAUACAAUGGACAAU hsa-mir-2267 hsa-mir- 1124 cgUgUcAUCCCCAGAUACAAUGGACAAUaUgcUaUUaUa
2355 aUcgUaUggcaUUgUccUUgcUgUUUggagaUaaUacU
831 AUUGUCCUUGCUGUUUGGAGAU hsa-mir-2267* pending 1125 cgUgUcaUccccagaUacaaUggacaaUaUgcUaUUaUaaUcg
UaUggcAUUGUCCUUGCUGUUUGGAGAUaaUacU
832 CACAGGCUUAGAAAAGACAGU hsa-mir-2268 hsa-mir- 1126 UaagUgUaaacUUaaggacUgUcUUUUcUaagccUgUgccUU
4438 gccUUUccUUUggCACAGGCUUAGAAAAGACAGUcUUUa
agUUUacacUUc
833 UCGAGGACUGGUGGAAGGGCCU hsa-mir-2269 hsa-mir- 1127 UcUcagagUCGAGGACUGGUGGAAGGGCCUUUccccUca
3131 gaccaaggcccUggccccagcUUcUUcUcagagU
834 GUGACUGAUACCUUGGAGGCAU hsa-mir-2270 hsa-mir- 1128 ccaGUGACUGAUACCUUGGAGGCAUUUUaUcUaagaUac
4439 acacaaagcaaaUgccUcUaaggUaUcagUUUaccaggcca
835 AGGGCUGGACUCAGCGGCGGAG hsa-mir-2271 pending 1129 gcgcagAGGGCUGGACUCAGCGGCGGAGcUggcUgcUggc
cUcagUUcUgccUcUgUccaggUccUUgUga
836 UGUCGUGGGGCUUGCUGGCUUG hsa-mir-2272 hsa-mir- 1130 cUcUcaccaagcaagUgcagUggggcUUgcUggcUUgcaccgUg
4440 acUcccUcUcaccaagcaagUGUCGUGGGGCUUGCUGGCU
UGcacUgUgaagaU
837 ACAGGGAGGAGAUUGUA hsa-mir-2273 hsa-mir- 1131 cagagUcUccUUcgUgUacagggaggagacUgUacgUgagagaU
4441 agUcagaUccgcaUgUUagagcagagUcUccUUcgUgUACAG
GGAGGAGAUUGUAc
838 ACUGGACUUGGAGGCAGAA hsa-mir-2274 hsa-mir- 1132 UggUcaUUgagUcUUcaaggcUagUggaaagagcACUGGACU
378b UGGAGGCAGAAagaccc
839 GCCGGACAAGAGGGAGG hsa-mir-2275 hsa-mir- 1133 gcgcccUcccUcUcUccccggUgUgcaaaUgUgUgUgUgcggUg
4442 UUaUGCCGGACAAGAGGGAGGUg
840 AUACACAUACACGCAACACACA hsa-mir-2276 hsa-mir- 1134 aUgUgUgUgUaUaUgUgUgUUgcaUgUgUgUaUaUgUgUg
466 UaUaUaUgUacacAUACACAUACACGCAACACACAUaUa
UacaUgcac
841 UUGGAGGCGUGGGUUUU hsa-mir-2277 hsa-mir- 1135 ggUgggggUUGGAGGCGUGGGUUUUagaaccUaUcccUU
4443 UcUagcccUgagca
842 AAUUCCCUUGUAGAUAACCCGG hsa-mir-2278 hsa-mir- 1136 cgaUcacUagaUUaUcUacaagggaaUUUUUUUUUaaUUU
3938 aaaaAAUUCCCUUGUAGAUAACCCGGUggUca
843 AAGACUGGAGACAAAGUGGGAG hsa-mir-2279 pending 1137 CACCACCAAAAUCUCCAGGGGCAUCGUUGAAAUCGUA
AGGGAUGUGCAGCUCAUUAAGACUGGAGACAAAGUG
GGAG
844 CUGACUGAAUAGGUAGGGUCAU hsa-mir-2280 hsa-mir- 1138 aaaCUGACUGAAUAGGUAGGGUCAUUUUUcUgUgacUg
3136 cacaUggcccaaccUaUUcagUUagUUc
845 CUCGAGUUGGAAGAGGCG hsa-mir-2281 hsa-mir- 1139 gUgacgacUggccccgccUcUUccUcUcggUcccaUaUUgaaCU
4444 CGAGUUGGAAGAGGCGagUccggUcUcaaa
846 AGAUUGUUUCUUUUGCCGUGCA hsa-mir-2282 hsa-mir- 1140 UUccUgcAGAUUGUUUCUUUUGCCGUGCAagUUUaagU
4445 UUUUgcacggcaaaagaaacaaUccagagggU
847 CACGGCAAAAGAAACAAUCCA hsa-mir-2282* has-mir- 1141 UUccUgcagaUUgUUUcUUUUgccgUgcaagUUUaagUUU
4445 UUgCACGGCAAAAGAAACAAUCCAgagggU
848 CAGGGCUGGCAGUGACAUGGGU hsa-mir-2283 hsa-mir- 1142 cUggUccaUUUcccUgccaUUcccUUggcUUcaaUUUacUcc
4446 CAGGGCUGGCAGUGACAUGGGUcaa
849 GGUGGGGGCUGUUGUUU hsa-mir-2284 hsa-mir- 1143 gUUcUagagcaUggUUUcUcaUcaUUUgcacUacUgaUacU
4447 UggggUcagaUaaUUgUUUgUGGUGGGGGCUGUUGUUU
gcaUUgUaggaU
850 UGGGGAGGUGUGGAGUCAGCAU hsa-mir-2285 pending 1144 gggcaUGGGGAGGUGUGGAGUCAGCAUggggcUaggaggc
cccgcgcUgacccgccUUcUccgcagcUg
accUgagcaccaUUUacUgagUccUUUgUUcUcUacUagUUU
851 GCAGAGAACAAAGGACUCAGU hsa-mir-2286 hsa-mir- 1145 gUagUagUUcgUaGCAGAGAACAAAGGACUCAGUaaaUg
3919 gUgcUcagga
852 GGCUCCUUGGUCUAGGGGUA hsa-mir-2287 hsa-mir- 1146 aggagUgaccaaaagacaagagUgcgagccUUcUaUUaUgcccag
4448 acagggccaccagagGGCUCCUUGGUCUAGGGGUAaUgcca
853 CGUCCCGGGGCUGCGCGAGGCA hsa-mir-2288 hsa-mir- 1147 agcagcccUcggcggcccggggggcgggcggcggUgccCGUCCCG
4449 GGGCUGCGCGAGGCAcaggcg
854 AAAGGUAAUUGUGGUUUCUGC hsa-mir-2289- hsa-mir- 1148 gUgcAAAGGUAAUUGUGGUUUCUGCUUUUaaaggUaaU
1 548ag-1 ggcaaaUaUUacaUUUacUUUUgcacca
855 AAAGGUAAUUGUGGUUUCUGC hsa-mir-2289- hsa-mir- 1149 UgcAAAGGUAAUUGUGGUUUCUGCcaUUgaaagUaaagg
2 548ag-2 caagaaccUcaaUUaccUUUgcagc
856 UGGGGAUUUGGAGAAGUGGUGA hsa-mir-2290 hsa-mir- 1150 UgUcUGGGGAUUUGGAGAAGUGGUGAgcgcaggUcUUU
4450 ggcaccaUcUccccUggUcccUUggcU
857 AAAAGUGAUUGCAGUGUUUG hsa-mir-2291 hsa-mir- 1151 aggUUggUgcAAAAGUGAUUGCAGUGUUUGccaaUaaaa
548ah gUaaUgacaaaaacUgcagUUacUUUUgcaccagccc
858 UGGUAGAGCUGAGGACA hsa-mir-2292 hsa-mir- 1152 UcUgUaccUcagcUUUgcUcccaaccaaccacUUccacaUgUU
4451 UUgcUGGUAGAGCUGAGGACAgc
859 UUGAAUUCUUGGCCUUAAGUGAU hsa-mir-2293 hsa-mir- 1153 UggaUcacUUgaggccaagagUgcaaggcUgUagUgUgcacagc
4452 cUUGAAUUCUUGGCCUUAAGUGAUccc
860 UAGGAGCUCAACAGAUGCCUGU hsa-mir-2294 hsa-mir- 1154 UcagagUAGGAGCUCAACAGAUGCCUGUUgacUgaaUaa
3139 UaaacaggUaUcgcaggagcUUUUgUUaUgU
861 AGCUUUUGGGAAUUCAGGUAG hsa-mir-2295 hsa-mir- 1155 UgUccUcUUgaggUaccUgaaUUaccaaaagcUUUaUgUaUU
3140 cUgaagUUaUUgaaaaUaagAGCUUUUGGGAAUUCAGGU
AGUUcaggagUgacU
862 GAGCUUGGUCUGUAGCGGUU hsa-mir-2296 hsa-mir- 1156 UggaGAGCUUGGUCUGUAGCGGUUUccUUggggcaggUg
4453 gggacUgcUccUUUgggaggaaggaggaggcccaggccgcgUcUU
cagg
863 GGAUCCGAGUCACGGCACCA hsa-mir-2297 hsa-mir- 1157 ccGGAUCCGAGUCACGGCACCAaaUUUcaUgcgUgUccgU
4454 gUgaagagaccacca
864 CAAAAGUGAUCGUGGUUUUUG hsa-mir-2298 hsa-mir- 1158 gUggUgCAAAAGUGAUCGUGGUUUUUGcaaUUUUUUaa
548t UgacaaaaaccacaaUUacUUUUgcaccaa
865 AGGGUGUGUGUGUUUUU hsa-mir-2299 hsa-mir- 1159 agaAGGGUGUGUGUGUUUUUccUgagaaUaagagaaggaa
4455 ggacagccaaaUUcUUca
866 CCUGGUGGCUUCCUUUU hsa-mir-2300 hsa-mir- 1160 aUgaaCCUGGUGGCUUCCUUUUcUgggaggaagUUagggU
4456 Uca
867 UCACAAGGUAUUGACUGGCGUA hsa-mir-2301 hsa-mir- 1161 ggagUacUccagUcaaUaccgUgUgagUUagaaaagcUcaaUU
4457 CACAAGGUAUUGACUGGCGUAUUca
868 AGAGGUAGGUGUGGAAGAA hsa-mir-2302 hsa-mir- 1162 gagcgcacAGAGGUAGGUGUGGAAGAAagUgaaacacUaU
4458 UUUaggUUUUagUUacacUcUgcUgUggUgUgcUg
869 CCAGGAGGCGGAGGAGGUGGAG hsa-mir-2303 hsa-mir- 1163 acCCAGGAGGCGGAGGAGGUGGAGgUUgcagUgagccaag
4459 aUcgUggcacUgacUccagccUgggg
870 GUGGAGGACUGAGAAGGUGAG hsa-mir-2304 pending 1164 aUgaUGUGGAGGACUGAGAAGGUGAGgcagUUUUgcccc
gUgcUgccUUccaccggUUaagaccUccaaaaUcga
871 ACUGACAGGAGAGCAUUUUGA hsa-mir-2305 hsa-mir- 1165 ggacaaaaUUaaaaUgcUcUUcUgUcaUUgUaaUagUUcaUa
3660 UgggcACUGACAGGAGAGCAUUUUGAcUUUgUca
872 AGCGCGGGCUGAGCGCUGCCAG hsa-mir-2306 hsa-mir- 1166 ggUUcacUggUcgUgcUUccUgcgggcUgAGCGCGGGCUGA
2277 GCGCUGCCAGUcagcg
873 AAGCUCGGGCGCUCCGGCUGU hsa-mir-2307 pending 1167 gcUcagUcagcUgggccgccUcagcUcUcggagUaggAAGCUCG
GGCGCUCCGGCUGUaaggagcc
874 AUAGUGGUUGUGAAUUUACCUU hsa-mir-2308 hsa-mir- 1168 gUUUUUUgcccAUAGUGGUUGUGAAUUUACCUUcUccU
4460 cUUUgcagUgaUaaaggaggUaaaUUcacaaccacUgUgggcag
aaac
875 GAUUGAGACUAGUAGGGCUAGGC hsa-mir-2309 hsa-mir- 1169 gagUaggcUUaggUUaUgUacgUagUcUaggccaUacgUgUU
4461 ggaGAUUGAGACUAGUAGGGCUAGGCcUacUg
876 CUACCCCAGGAUGCCAGCAUAG hsa-mir-2310 pending 1171 aUagcUggUUggcaUUcUggcccUggUUcaUgccaacUcUUg
UgUUgaCUACCCCAGGAUGCCAGCAUAGUUgc
877 ACUGGACUUGGUGUCAGAUGG hsa-mir-2311 hsa-mir- 1171 acaggaacACUGGACUUGGUGUCAGAUGGgaUgagcccUg
378h gcUcUgUUUccUagcagcaaUcUgaUcUUgagcUagUcacUgg
878 GACAAUGAUGAGAAGACCUGAG hsa-mir-2312 pending 1172 gggGACAAUGAUGAGAAGACCUGAGgaUUUgcagccccca
gcccUgggUUcaagUcccagcUcUaccccUUcUUggcccc
879 ACCGCUCGAUCUUGGGACC hsa-mir-2313 pending 1173 gUUUcACCGCUCGAUCUUGGGACCcaccgcUgcccUcagcU
ccgagUccagggcgaggUaagggcUggagUcgggcagga
880 UAGUGGAUGAUGGAGACUCGGU hsa-mir-2314 hsa-mir- 1174 aUUgaggcacUgggUAGUGGAUGAUGGAGACUCGGUacc
3691 cacUgcUgagggUggggaccaagUcUgcgUcaUccUcUccUcag
UgccUcaaa
881 CUCGGGAGCGUUAGAGAUGGA hsa-mir-2315 pending 1175 gUCUCGGGAGCGUUAGAGAUGGAgacUaacgUcUUccaa
gggagaUUgcgUcUccacUUUcacccUggUacUgagag
882 GGCUGGAGCGAGUGCAGUGGUG hsa-mir-2316 hsa-mir- 1176 UgcccaGGCUGGAGCGAGUGCAGUGGUGcagUcagUccU
3135b agcUcacUgcagccUcgaacUccUgggcU
883 UCAGGUGUGGAAACUGAGGCAG hsa-mir-2317 hsa-mir- 1177 UUUUCAGGUGUGGAAACUGAGGCAGgaggcagUgaagUa
3934 acUUgcUcaggUUgcacagcUgggaagU
884 UGACACGGAGGGUGGCUUGGGAA hsa-mir-2318 hsa-mir- 1178 cUUcccagcUgcccUaagUcaggagUggcUUUccUGACACGG
4462 AGGGUGGCUUGGGAAa
885 GAAGAUGGUGCUGUGCUGAGGA hsa-mir-2319 pending 1179 ggUGAAGAUGGUGCUGUGCUGAGGAaaggggaUgcagagc
ccUgcccagcaccaccaccUccUaUg
886 AAAGACUGCAAUUACUUUUGCG hsa-mir-2320 hsa-mir- 1180 aUggUgcaaaagUaaUgUggUUUUUUUcUUUacUUUUaaU
548u ggcAAAGACUGCAAUUACUUUUGCGcca
887 GAGACUGGGGUGGGGCC hsa-mir-2321 hsa-mir- 1181 aaUagaUUaUUggUcaccaccUccagUUUcUgaaUUUgUGA
4463 GACUGGGGUGGGGCCUgagaaUUUgc
888 AAGGUUUGGAUAGAUGCAAUA hsa-mir-2322 hsa-mir- 1182 ggaaccUUagUAAGGUUUGGAUAGAUGCAAUAaagUaUg
4464 UccacagcUgaaaggacaUacUUUaUUgcaUgUaUccaaaccU
UacUaaUUca
889 AAAGGUAAUUGCAGUUUUUCCC hsa-mir-2323 hsa-mir- 1183 gUaUUaggUUggUgcAAAGGUAAUUGCAGUUUUUCCCa
548ai UUUaaaaUaUggaaaaaaaaaUcacaaUUacUUUUgcaUcaa
ccUaaUaa
890 AGGGGACCAAAGAGAUAUAUAG hsa-mir-2324 hsa-mir- 1184 gaaacUacacUUUaAGGGGACCAAAGAGAUAUAUAGaUa
3144 UcagcUaccUaUaUaccUgUUcggUcUcUUUaaagUgUagU
UUa
891 UAAAAACUGCAAUUACUUUUA hsa-mir-2325- hsa-mir- 1185 aUUggUgUaaaagUaaUUgcaggUUaUgccaUUaaaagUaaU
1 548aj-1 ggUAAAAACUGCAAUUACUUUUAcacUaac
892 UAAAAACUGCAAUUACUUUUA hsa-mir-2325- hsa-mir- 1186 aaggUaUUaggUUggUgcaaaagUaaUUgcagUUUUUgcUa
2 548aj-2 UUacUUUUaaUggUAAAAACUGCAAUUACUUUUAcacca
accUaaUaUUUa
893 CUCAAGUAGUCUGACCAGGGGA hsa-mir-2326 hsa-mir- 1187 caUgUgUccccUggcacgcUaUUUgaggUUUacUaUggaacC
4465 UCAAGUAGUCUGACCAGGGGAcacaUga
894 UCUGGCUGAGGAGGAAGUGGAG hsa-mir-2327- pending 1188 gcUcUagUagccacagccaUccccUagagggaUCUGGCUGAGG
1 AGGAAGUGGAGg
895 UCUGGCUGAGGAGGAAGUGGAG hsa-mir-2327- pending 1189 ccgccUcagUggcUUccUccacagccaccUccggagggaUCUGGC
2 UGAGGAGGAAGUGGAGgUgUcacUgg
896 GGCGACAAAACGAGACCCUGU hsa-mir-2328 hsa-mir- 1190 cUgGGCGACAAAACGAGACCCUGUcUUUUUUUUUUUc
1273c UgagacagagUcUcgUUcUgUUgcccaa
897 GGGUGCGGGCCGGCGGGG hsa-mir-2329 hsa-mir- 1191 acgcGGGUGCGGGCCGGCGGGGUagaagccacccggcccggc
4466 ccggcccggcga
898 CCUGCUGGUCAGGAGUGGAUAC hsa-mir-2330 hsa-mir- 1192 gccaUUCCUGCUGGUCAGGAGUGGAUACUggagcaaUag
3692 aUacagUUccacacUgacacUgcagaagUgga
899 CAUGCUAGGAUAGAAAGAAUGG hsa-mir-2331 hsa-mir- 1193 aUUUUcUUUgcUaagUcccUUcUUUcUaUccUagUaUaac
3146 UUgaagaaUUcaaaUagUCAUGCUAGGAUAGAAAGAAUG
GgacUUggccagggaagaa
900 AAUGUGGAAGUGGUCUGAGGCA hsa-mir-2332 pending 1194 gaaUagaaagAAUGUGGAAGUGGUCUGAGGCAUaUagag
UaUaUgccaagaacacUaccaUa
901 GCAAAGUGAUGAGUAAUACUGG hsa-mir-2333 hsa-mir- 1195 acagUaacUUUUaUUcUcaUUUUccUUUUcUcUaccUUgU
3609 agagaaGCAAAGUGAUGAGUAAUACUGGcUgg
902 UGGCGGCGGUAGUUAUGGGCUU hsa-mir-2334 hsa-mir- 1196 UggUGGCGGCGGUAGUUAUGGGCUUcUcUUUcUcacca
4467 gcagccccUgggccgccgccUcccU
903 AGAGCAGAAGGAUGAGAU hsa-mir-2335 hsa-mir- 1197 agUcUUcUccUggggcUUUggUggcUaUggUUgacUgggccac
4468 UcAGAGCAGAAGGAUGAGAUg
904 UGGAAUGGCCUGAAGGUGGA hsa-mir-2336 pending 1198 ccUggcagcccUcUggccUagUUcccaccacacaUgaggUggUG
GAAUGGCCUGAAGGUGGAacaga
905 GCUCCCUCUAGGGUCGCUCGGA hsa-mir-2337 hsa-mir- 1199 ccgacgcggagagcggcUcUaggUgggUUUggcggcggcgaggaca
4469 ccgccGCUCCCUCUAGGGUCGCUCGGAgcgUga
906 UGGCAAACGUGGAAGCCGAGA hsa-mir-2338 hsa-mir- 1200 cgagccUcUUUcggcUUUccagUUUgUcUcggUccUUUggaa
4470 cgUGGCAAACGUGGAAGCCGAGAgggcUcU
907 UUUGUAUGGAUAUGUGUGUGUA hsa-mir-2339 hsa-mir- 1201 aUaUacaUacaUgUacacacacaUgUcaUccacacacaUacaUa
3149 UaUaUaUgUUUGUAUGGAUAUGUGUGUGUAUgUgUg
UgUaUac
908 UGAGGAGAUCGUCGAGGUUGGC hsa-mir-2340 hsa-mir- 1202 aaagcaggccaaccUcgaggaUcUccccagccUUggcgUUcaggU
3150b gcUGAGGAGAUCGUCGAGGUUGGCcUgcUUc
909 UGGGAACUUAGUAGAGGUUUAA hsa-mir-2341 hsa-mir- 1203 ccaaaUUUaaaacUUaaaccUcUacUaagUUUccaUgaaaaga
4471 acccaUGGGAACUUAGUAGAGGUUUAAgUUUUaaaUUU
ga
910 GGUGGGGGGUGUUGUUUU hsa-mir-2342- hsa-mir- 1204 UggcagacccUUgcUcUcUcacUcUcccUaaUggggcUgaagac
1 4472-1 agcUcaggggcagGGUGGGGGGUGUUGUUUUUgUUU
911 GGUGGGGGGUGUUGUUUU hsa-mir-2342- hsa-mir- 1205 UggUgggGGUGGGGGGUGUUGUUUUUgUUUUUgagaca
2 4472-2 gagUcUUgcUccgUcgcccaggccggagU
912 CUAGUGCUCUCCGUUACAAGUA hsa-mir-2343 hsa-mir- 1206 aaggaacaggggacacUUgUaaUggagaacacUaagcUaUggac
4473 UgcUaUggacUgCUAGUGCUCUCCGUUACAAGUAUcccc
UgUUaccU
913 CACUUGUAAUGGAGAACACUAA hsa-mir-2344 pending 1207 aaaggaacaggggaCACUUGUAAUGGAGAACACUAAgcUa
UggacUgcUaUggacUgcUagUgcUcUccgUUacaagUaUccc
cUgUUaccUUg
914 UUGUGGCUGGUCAUGAGGCUAA hsa-mir-2345 hsa-mir- 1208 UUgccUaccUUgUUagUcUcaUgaUcagacacaaaUaUggcUc
4474 UUUGUGGCUGGUCAUGAGGCUAAcaaggUaggcac
915 CCCUGGGGUUCUGAGGACAUG hsa-mir-2346 pending 1209 aUgcUgCCCUGGGGUUCUGAGGACAUGcUcUgacUcccc
UgaUgUccUcUgUUccUcaggUgcUgggcga
916 CAAGGGACCAAGCAUUCAUUAU hsa-mir-2347 hsa-mir- 1210 aUcUcaaUgagUgUgUggUUcUaaaUgacUcaUagUCAAGG
4475 GACCAAGCAUUCAUUAUgaa
917 CAGGAAGGAUUUAGGGACAGGC hsa-mir-2348 hsa-mir- 1211 aaaagccUgUcccUaagUcccUcccagccUUccagagUUggUgc
4476 CAGGAAGGAUUUAGGGACAGGCUUUg
918 CUAUUAAGGACAUUUGUGAUUC hsa-mir-2349 hsa-mir- 1212 UccUccUcccaUcaaUcacaaaUgUccUUaaUggcaUUUaagg
4477a aUUgCUAUUAAGGACAUUUGUGAUUCacgggaggaggU
919 AUUAAGGACAUUUGUGAUUGAU hsa-mir-2350 hsa-mir- 1213 accUccUcccgUgaaUcacaaaUgUccUUaaUagcaaUccUUaa
4477b aUgccAUUAAGGACAUUUGUGAUUGAUgggaggagga
920 AAAAGGCAUAAAACCAAGACA hsa-mir-2351- hsa-mir- 1214 cUUUUUUUgUUgcUUgUcUUggUUUUaUgccUUUUaUg
1 3910-2 UgccUUgaUaUAAAAGGCAUAAAACCAAGACAagcaacag
aaaaac
921 AAAAGGCAUAAAACCAAGACA hsa-mir-2351- hsa-mir- 1215 cUgUcagUUUUUcUgUUgcUUgUcUUggUUUUaUgccUU
2 3910-1 UUaUaUcaaggcacaUAAAAGGCAUAAAACCAAGACAagc
aacaa
922 GAGGCUGAGCUGAGGAG hsa-mir-2352 hsa-mir- 1216 ggccGAGGCUGAGCUGAGGAGccUccaaaccUgUagacagg
4478 gUcaUgcagUacUaggggcgagccUcaUccccUgcagcccUggcc
923 CUGGGAGGUGUGAUAUUGUGGU hsa-mir-2353 hsa-mir- 1217 gggaggUgUgaUaUcgUggUUccUgggaggUgUgaUaUcgUg
3689c gUUcCUGGGAGGUGUGAUAUUGUGGUUccU
924 UAAAAACUGCAAUUACUUUC hsa-mir-2354- hsa-mir- 1218 aUgccaaaUaUUaggUUggcacaaaagUaaUUgUggcUUUUg
1 548x-2 ccaUUaaaagUaaUggUAAAAACUGCAAUUACUUUCgUgc
caaccUaaUaUUUgUgUg
925 UAAAAACUGCAAUUACUUUC hsa-mir-2354- hsa-mir- 1219 agUgcaaaagUaaUUgcagUUUUUgcgUUacUUUcaaUcgU
2 548x-1 AAAAACUGCAAUUACUUUCacacc
926 UGUGAUAUCAUGGUUCCUGGGA hsa-mir-2355- hsa-mir- 1220 gcUcccUgggaggUGUGAUAUCAUGGUUCCUGGGAggUg
1 3689a UgaUccUgUgcUUccUgggaggUgUgaUaUcgUggUUccUgg
gagg
927 GGGAGGUGUGAUCUCACACUCG has-mir-2356- hsa-mir- 1221 UGGGAGGUGUGAUCUCACACUCGcUgggaggUgUgcUaU
1 3689d-1 cgUcUUccccgggaggUgUgaUccUgUUcUUccUg
928 CUGGGAGGUGUGAUAUUGUGGU mir-2355-2* has-mir- 1222 gggaggUGUGAUAUCAUGGUUCCUGGGAggUgUgaUccc
3689b-1 gUgcUUcCUGGGAGGUGUGAUAUUGUGGUUccU
929 UGUGAUAUCAUGGUUCCUGGGA hsa-mir-2355- hsa-mir- 1223 gggaggUGUGAUAUCAUGGUUCCUGGGAggUgUgaUccc
2 3689b-1 gUgcUUcCUGGGAGGUGUGAUAUUGUGGUUccU
930 GGGAGGUGUGAUCUCACACUCG has-mir-2356- hsa-mir- 1224 acUGGGAGGUGUGAUCUCACACUCGcUgggaggUgUgcU
2 3689d-2 aUcgUcUUcccUgggaggUgUgaUccUgUUcUUccUgagcg
931 UGUGAUAUCAUGGUUCCUGGGA hsa-mir-2355- has-mir- 1225 gggaggUGUGAUAUCAUGGUUCCUGGGAggUaUgaUaUc
3 3689b-2 gUggUUccUgggaggUgUgaUcccgUgcUcccU
932 UGUGAUAUCGUGCUUCCUGGGA hsa-mir-2355b hsa-mir- 1226 aggUGUGAUAUCGUGCUUCCUGGGAcgUgUgaUgcUgUg
3689f cUUccUgggaggUgUgaUcccacacUc
933 CGCGCGGCCGUGCUCGGAGCAG hsa-mir-2356 hsa-mir- 1227 gaaaccaagUccgagcgUggcUggcgcgggaaagUUcgggaacgC
4479 GCGCGGCCGUGCUCGGAGCAGcgcca
934 CCAGGCUCUGCAGUGGGAACU hsa-mir-2357a hsa-mir- 1228 ccUgUUccgggcaUcaccUcccacUgcagagccUggggagccggac
3155a agcUcccUUcCCAGGCUCUGCAGUGGGAACUgaUgccUgg
aacagU
935 CCAGGCUCUGCAGUGGGA hsa-mir-2357b hsa-mir- 1229 ccacUgcagagccUgggaagggagcUgUccggcUccCCAGGCUC
3155b UGCAGUGGGAgU
936 AAAAGUAACUGCGGUUUUUGA hsa-mir-2358 hsa-mir- 1230 gUgcAAAAGUAACUGCGGUUUUUGAgaagUaaUUgaaaa
548ak ccgcaaUUacUUUUgcag
937 AGCCAAGUGGAAGUUACUUUA hsa-mir-2359 hsa-mir- 1231 gcagaggUgagUUgaccUccacagggccacccagggagUaagUAG
4480 CCAAGUGGAAGUUACUUUAccUcUgU
938 GGAGUGGGCUGGUGGUU hsa-mir-2360 hsa-mir- 1232 GGAGUGGGCUGGUGGUUUUUUaagaggaagggagaccUa
4481 agcUagcacaUgagcacgcUc
939 GAGCGAUCCGAGGGACUG hsa-mir-2361 pending 1233 cggcUUcccgcggUccccggUgcUgaggagaGAGCGAUCCGAG
GGACUGcgccgcc
940 AAGGGCUUCCUCUCUGCAGGAC hsa-mir-2362- hsa-mir- 1234 acaUUaUUcaggccggUccUgcagagaggaagcccUUccaaUacc
1 3158-2 UgUaagcagAAGGGCUUCCUCUCUGCAGGACcggccUgaa
UaaUga
941 AAGGGCUUCCUCUCUGCAGGAC hsa-mir-2362- hsa-mir- 1235 ggaUcaUUaUUcaggccggUccUgcagagaggaagcccUUcUgc
2 3158-1 UUacaggUaUUggAAGGGCUUCCUCUCUGCAGGACcggcc
UgaaUaaUgUaaUca
942 AACCCAGUGGGCUAUGGAAAUG hsa-mir-2363 hsa-mir- 1236 agUgagcAACCCAGUGGGCUAUGGAAAUGUgUggaagaU
4482 ggcaUUUcUaUUUcUcagUggggcUcUUacc
943 GGGGUGGUCUGUUGUUG hsa-mir-2364 hsa-mir- 1237 aaaaaacaacaUacUUagUgcaUacccaUaUaaUaUUaGGGG
4483 UGGUCUGUUGUUGUUUUUcU
944 AAAAGGCGGGAGAAGCCCCA hsa-mir-2365 hsa-mir- 1238 gggUUUccUcUgccUUUUUUUccaaUgaaaaUaacgaaaccU
4484 gUUaUUUcccaUUgagggggaAAAAGGCGGGAGAAGCCCC
A
945 UGGCUGUUGGAGGGGGCAGGCU hsa-mir-2366 pending 1239 ggagccagcccUccUcccgcacccaaacUUggagcacUUgaccUU
UGGCUGUUGGAGGGGGCAGGCUcg
946 UAACGGCCGCGGUACCCUAA hsa-mir-2367 hsa-mir- 1240 agaggcaccgccUgcccagUgacaUgcgUUUAACGGCCGCGG
4485 UACCCUAAcUgUgca
947 UAGGAUUACAAGUGUCGGCCAC hsa-mir-2368 hsa-mir- 1241 ccUAGGAUUACAAGUGUCGGCCACgggcUgggcacagUggc
3159 UcacgccUgUaaUcccagc
948 GCUGGGCGAGGCUGGCA hsa-mir-2369 hsa-mir- 1242 gcaUGCUGGGCGAGGCUGGCAUcUagcacaggcggUagaUg
4486 cUUgcUcUUgccaUUgcaaUga
949 AGAGCUGGCUGAAGGGCAG hsa-mir-2370 hsa-mir- 1243 acUgUccUUcagccAGAGCUGGCUGAAGGGCAGaagggaac
4487 UgUccUUcagccagagcUggcUgaagggcaga
950 AGGGGGCGGGCUCCGGCG hsa-mir-2371 hsa-mir- 1244 ggUAGGGGGCGGGCUCCGGCGcUgggaccccacUagggUgg
4488 cgccUUggccccgccccgccc
951 GCCGAGAGUCGUCGGGGUU hsa-mir-2372 pending 1245 UcGCCGAGAGUCGUCGGGGUUUccUgcUUcaacagUgcU
Uggacggaacccggcgc
952 UGGGGCUAGUGAUGCAGGACG hsa-mir-2373 hsa-mir- 1246 gggggUGGGGCUAGUGAUGCAGGACGcUggggacUggaga
4489 agUccUgccUgacccUgUccca
953 UGUGACUUUAAGGGAAAUGGCG hsa-mir-2374 hsa-mir- 1247 UggaaacUGUGACUUUAAGGGAAAUGGCGcacagcagacc
3164 cUgcaaUcaUgccgUUUUgcUUgaagUcgcagUUUccc
954 UCUCAGGAGUAAAGACAGAGUU hsa-mir-2375 hsa-mir- 1248 aacUUgaaggUagggaacUcUgUcUUcacUcaUgagUaccUUc
3664 caacacgagcUCUCAGGAGUAAAGACAGAGUUcccUaccU
UcaaUgU
955 AGGUGGAUGCAAUGUGACCUCA hsa-mir-2376 hsa-mir- 1249 caAGGUGGAUGCAAUGUGACCUCAacUcUUggUccUcUg
3165 aggUcacaUUgUaUccaccUUa
956 AACGGCAAUGACUUUUGUACCA hsa-mir-2377 hsa-mir- 1250 ggUcggUgcaaaagUaaUUgcUgUUUUUgccaUUaaaaaUaa
548a1 UggcaUUaaaagUaaUggcaaaAACGGCAAUGACUUUUGU
ACCAaUcUaaUaUcU
957 UCUGGUAAGAGAUUUGGGCAUA hsa-mir-2378 hsa-mir- 1251 aUagUUUcUgcaaUgcUcaaaUcUcUggccaaagaccagaacU
4490 UaaUggUcUCUGGUAAGAGAUUUGGGCAUAUUagaaac
Uaa
958 AAUGUGGACUGGUGUGACCAAA hsa-mir-2379- hsa-mir- 1252 acaUUUggUcacaccagUccacaUUaacgUggaccagacaaUaU
1 4491 UAAUGUGGACUGGUGUGACCAAAa
959 AAUGUGGACUGGUGUGACCAAA hsa-mir-2379- pending 1253 accUggacaUUUggUcacaccagUccacaUUaacgUggaccagac
2 aaUaUUAAUGUGGACUGGUGUGACCAAAagUccaggc
960 GGGGCUGGGCGCGCGCC hsa-mir-2380 hsa-mir- 1254 cUgcagcgUgcUUcUccaggccccgcgcgcggacagacacacgga
4492 caagUcccgccaGGGGCUGGGCGCGCGCCagccgg
961 AGAAGGCCUUUCCAUCUCUGU hsa-mir-2381 hsa-mir- 1255 ccagagaUgggaaggccUUccggUgaUUaUcacagccaUgccUU
4493 UaccUccAGAAGGCCUUUCCAUCUCUGUc
962 CCAGACUGUGGCUGACCAGAGG hsa-mir-2382 hsa-mir- 1256 agUUUUagUUacccUggUcaUcUgcagUcUgaaaaUacaaaa
4494 UggaaaaUUCCAGACUGUGGCUGACCAGAGGUaacUgaa
acc
963 AGGAGAAGCAGGAGCUGU hsa-mir-2383 pending 1257 cUgAGGAGAAGCAGGAGCUGUcUUggUacaUUcaggUcac
Ug
964 AAUGUAAACAGGCUUUUUGCU hsa-mir-2384 hsa-mir- 1258 aagaAAUGUAAACAGGCUUUUUGCUcagUggagUUaUUU
4495 UgagcaaaaagcUUaUUUacaUUUcUg
965 GAGGAAACUGAAGCUGAGAGGG hsa-mir-2385 hsa-mir- 1259 ACAUCAGCUCAUAUAAUCCUCGAAGCUGCCUUUAGAA
4496 AUGAGGAAACUGAAGCUGAGAGGG
966 CUCCGGGACGGCUGGGC hsa-mir-2386 hsa-mir- 1260 acCUCCGGGACGGCUGGGCgccggcggccgggagaUccgcgcU
4497 UccUgaaUcccggccggcccgcccggcgcccgUccgcccgcgggUc
967 UGGGCUGGCAGGGCAAGUGCUG hsa-mir-2387 hsa-mir- 1261 agggcUGGGCUGGCAGGGCAAGUGCUGcagaUcUUUgUc
4498 UaagcagccccUgccUUggaUcUccca
968 GAGGCUGAAGGAAGAUGG hsa-mir-2388 hsa-mir- 1262 cUcaggcUcagUggUgcaUgcUUaUagUcccagccacUcUgGA
4419b GGCUGAAGGAAGAUGGcUUgagccU
969 AAGACUGAGAGGAGGGA hsa-mir-2389 hsa-mir- 1263 AAGACUGAGAGGAGGGAacUggUgagUUgUacaUagaaa
4499 UgcUUUcUaacUccUUgUcUcagUcUgUUU
970 UGAGGUAGUAGUUUCUU hsa-mir-2390 hsa-mir- 1264 caggagagaaagUacUgcccagaagcUaaagUgUagaUcaaacgc
4500 aUaaUggcUGAGGUAGUAGUUUCUUgaacUU
971 CGGCUGGGAGCCGAGGCGUCGG hsa-mir-2391 pending 1265 ggCGGCUGGGAGCCGAGGCGUCGGUgcagaccUggagacg
ggcaUgggggggcUgcggcUgcUggcUgUg
972 UAUGUGACCUCGGAUGAAUCA hsa-mir-2392 hsa-mir- 1266 UAUGUGACCUCGGAUGAAUCAcUgaaaUaUgUcUgagcU
4501 UcUgUUUcaUcagaUgUcacaUUUU
agccUUUagcaagUUgUaaUcUUUUUgcUgaUggagggUcU
973 GCUGAUGAUGAUGGUGCUGAAG hsa-mir-2393 hsa-mir- 1267 UgccUccaUggggaUgGCUGAUGAUGAUGGUGCUGAAGg
4502 c
974 AGAUGUAUGGAAUCUGUAUAU hsa-mir-2394 hsa-mir- 1268 gAGAUGUAUGGAAUCUGUAUAUaUcUaUaUaUaUgUgU
3171 aUaUaUagaUUccaUaaaUcUa
975 UUUAAGCAGGAAAUAGAAUUUA hsa-mir-2395 hsa-mir- 1269 acaaUgUagaUaUUUAAGCAGGAAAUAGAAUUUAcaUaU
4503 aaaUUUcUaUUUgUUUcUaUUUccUgcUUaaaUaUcUaca
UUgc
976 UGUGACAAUAGAGAUGAACAUG hsa-mir-2396 hsa-mir- 1270 cUaagaUaaUgUccUccaggUUcaUcUcUgUUgUcaUUUgU
4504 ggcaUggaccaUUUGUGACAAUAGAGAUGAACAUGgagga
UaUUaUcUUaa
977 AGGCUGGGCUGGGACGGA hsa-mir-2397 hsa-mir- 1271 ggAGGCUGGGCUGGGACGGAcacccggccUccacUUUcUg
4505 UggcaggUaccUccUccaUgUcggcccgccUUg
978 AAAUGGGUGGUCUGAGGCAA hsa-mir-2398 hsa-mir- 1272 UggccUcUgccaUcagaccaUcUgggUUcaagUUUggcUccaU
4506 cUUUaUgAAAUGGGUGGUCUGAGGCAAgUggUcU
979 UAGGAUGGGGGUGAGAGGUG hsa-mir-2399 hsa-mir- 1273 aUggUcccUcccaaUccagccaUUccUcagaccaggUggcUcccg
2392 agccaccccaggcUgUAGGAUGGGGGUGAGAGGUGcUag
980 CUGGGUUGGGCUGGGCUGGG hsa-mir-2400 hsa-mir- 1274 UcUgggcUgagccgagcUgggUUaagccgagCUGGGUUGGGC
4507 UGGGCUGGGU
981 AAGGGACUGGAGUGGAUUGGGU hsa-mir-2401 pending 1275 ggAAGGGACUGGAGUGGAUUGGGUacaUcUaUUaUagU
gggagcaccUacUacaacccgUcccUcaagagUcgagUcacc
982 AAGGGGCUGGAGUGGAUUGGGG hsa-mir-2402 pending 1276 cUggUgaaUcUgggUccgccagcccccagggAAGGGGCUGGA
GUGGAUUGGGGaaaUccaU
983 GCGGGGCUGGGCGCGCG hsa-mir-2403 hsa-mir- 1277 aggacccaGCGGGGCUGGGCGCGCGgagcagcgcUgggUgca
4508 gcgccUgcgccggcagcUgcaagggccg
984 ACUAAAGGAUAUAGAAGGUUUU hsa-mir-2404- hsa-mir- 1278 cUUUaaUacUaUcUcaaACUAAAGGAUAUAGAAGGUUU
1 4509-1 UcccUUUcUcUUgcccUgaaaccUUcUgUaUccUUUaUUUU
gagaUagUaUUagaa
985 ACUAAAGGAUAUAGAAGGUUUU hsa-mir-2404- hsa-mir- 1279 cUUUaaUacUaUcUcaaACUAAAGGAUAUAGAAGGUUU
2 4509-2 UcccUUUcUcUUgcccUgaaaccUUcUgUaUccUUUaUUUU
gagaUagUaUUagaa
986 ACUAAAGGAUAUAGAAGGUUUU hsa-mir-2404- hsa-mir- 1280 cUUUaaUacUaUcUcaaACUAAAGGAUAUAGAAGGUUU
3 4509-3 UcccUUUcUcUUgcccUgaaaccUUcUgUaUccUUUaUUUU
gagaUagUaUUagaa
987 AAGCAAUACUGUUACCUGAAAU hsa-mir-2405 hsa-mir- 1281 cUcaaagAAGCAAUACUGUUACCUGAAAUaggcUgcgaaga
3942 UaacagUaUUUcagaUaacagUaUUacaUcUUUgaa
988 UGAGGGAGUAGGAUGUAUGGUU hsa-mir-2406 hsa-mir- 1282 gUgUaUgUGAGGGAGUAGGAUGUAUGGUUgUUagaUa
4510 gacaacUacaaUcUUUUcUcacaacagacag
989 GAAGAACUGUUGCAUUUGCCCU hsa-mir-2407 hsa-mir- 1283 aaaaaaaagggaaaGAAGAACUGUUGCAUUUGCCCUgcac
4511 UcagUUUgcacagggUaaaUgcaaUagUUcUUcUUUcccUU
UUUUUa
990 CAGGGCCUCACUGUAUCGCCCA hsa-mir-2408 hsa-mir- 1284 cUcagcccgggcaaUaUagUgagaccUcgUcUcUacaaaaaaUU
4512 gagaCAGGGCCUCACUGUAUCGCCCAggcUgga
aUUcUaggUggggAGACUGACGGCUGGAGGCCCAUaagc
991 AGACUGACGGCUGGAGGCCCAU hsa-mir-2409 hsa-mir- 1285 UgUcUaaaacUUcggcccccagaUUUcUggUcUccccacUUcag
4513 aac
992 ACAGGCAGGAUUGGGGAA hsa-mir-2410 hsa-mir- 1286 gUUgagACAGGCAGGAUUGGGGAAacaUcUUUUaccUcg
4514 UcUcUUgccUgUUUUaga
993 AGGACUGGACUCCCGGCAGCCC hsa-mir-2411 hsa-mir- 1287 gcgggaggUgUaacAGGACUGGACUCCCGGCAGCCCcaggg
4515 caggggcgUggggagcUggUccUagcUcagcgcUcccgga
994 UAGUGAGUUAGAGAUGCAGAGC hsa-mir-2412 hsa-mir- 1288 agcgUUaccUggUAGUGAGUUAGAGAUGCAGAGCccUgg
3174 gcUccUcagcaaaccUacUggaUcUgca
995 ACUGGCCUGGGACUACCGGGGG hsa-mir-2413 hsa-mir- 1289 UcUgcagcUcccggcagccUcgggccacacUcccgggaUccccag
3176 ggACUGGCCUGGGACUACCGGGGGUggcggc
996 GGGAGAAGGGUCGGGGC hsa-mir-2414 hsa-mir- 1290 aGGGAGAAGGGUCGGGGCagggagggcagggcaggcUcUgg
4516 ggUggggggUcUgUgagUcagccacggcUcUgcccacgUcUcccc
997 UGGGGCGGAGCUUCCGGAGGCC hsa-mir-2415- hsa-mir- 1291 agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc
1 3180-1 gUGGGGCGGAGCUUCCGGAGGCCcc
998 UGGGGCGGAGCUUCCGGAGGCC hsa-mir-2415- hsa-mir- 1292 agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc
2 3180-2 gUGGGGCGGAGCUUCCGGAGGCCcc
999 UGGGGCGGAGCUUCCGGAGGCC hsa-mir-2415- hsa-mir- 1293 agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc
3 3180-3 gUGGGGCGGAGCUUCCGGAGGCCcc
1000 UGGGGCGGAGCUUCCGGAGGCC hsa-mir-2415- hsa-mir- 1294 agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc
4 3180-4 gUGGGGCGGAGCUUCCGGAGGCCcc
1001 UGGGGCGGAGCUUCCGGAGGCC hsa-mir-2415- hsa-mir- 1295 agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc
5 3180-5 gUGGGGCGGAGCUUCCGGAGGCCcc
1002 CUCGUGGGCUCUGGCCACGGC hsa-mir-2416 hsa-mir- 1296 UggagggcaUUaggcagUggccagagcccUgcagUgcUgggcaUg
3677 ggcUUCUCGUGGGCUCUGGCCACGGCccUgagcUccUcc
1003 AGAAGGGGUGAAAUUUAAACGU hsa-mir-2417- hsa-mir- 1297 gccaggaUcacagacgUUUaaaUUacacUccUUcUgcUgUgcc
1 3179-1 UUacagcagUAGAAGGGGUGAAAUUUAAACGUcUgUga
UccUggg
1004 AGAAGGGGUGAAAUUUAAACGU hsa-mir-2417- hsa-mir- 1298 gaUcacagacgUUUaaaUUacacUccUUcUgcUgUgccUUaca
2 3179-2 gcagUAGAAGGGGUGAAAUUUAAACGUcUgUgaUccUgg
g
1005 AGAAGGGGUGAAAUUUAAACGU hsa-mir-2417- hsa-mir- 1299 gccaggaUcacagacgUUUaaaUUacacUccUUcUgcUgUgcc
3 3179-3 UUacagcagUAGAAGGGGUGAAAUUUAAACGUcUgUga
UccUgg
1006 AAAUAUGAUGAAACUCACAGCUG hsa-mir-2418 hsa-mir- 1300 aggUAAAUAUGAUGAAACUCACAGCUGAGgagcUUagca
AG 4517 agUagcUaaggccagagcUUgUgUUUgggUggUgUggcUg
1007 GCUCAGGGAUGAUAACUGUGCUG hsa-mir-2419 hsa-mir- 1301 UgggggaaaagUgcUgggaUUgaUUagUgaUgUcUgcUgggga
AGA 4518 accgggGCUCAGGGAUGAUAACUGUGCUGAGAagcccccU
1008 CAGCAGUGCGCAGGGCUG hsa-mir-2420 hsa-mir- 1302 aaccUCAGCAGUGCGCAGGGCUGcacUgUcUccgUcUgcgg
4519 ccUgcagUaagcgggUa
1009 UUGGACAGAAAACACGCAGGAA hsa-mir-2421 hsa-mir- 1303 gUgUgccaccUgcgUgUUUUcUgUccaaaUcagaaaaggaUU
4520 UGGACAGAAAACACGCAGGAAgaaggaa
1010 GCUAAGGAAGUCCUGUGCUCAG hsa-mir-2422 hsa-mir- 1304 UcgGCUAAGGAAGUCCUGUGCUCAGUUUUgUagcaUca
4521 aaacUaggaUUUcUcUUgUUac
1011 CUGGACUGAGCCAUGCUACUGG hsa-mir-2423 hsa-mir- 1305 UgaggUUUCUGGACUGAGCCAUGCUACUGGcUUcUcUg
1269b gUUcUccagcUUacagaUggcUUaUcaUgggaccUcU
1012 UGACUCUGCCUGUAGGCCGGU hsa-mir-2424 hsa-mir- 1306 gcgggcgUUgccUgggggccUcgcagggggagaUccagcccaggcU
4522 ggUUccgcUGACUCUGCCUGUAGGCCGGUggcgUcUUcU
gg
1013 GACCGAGAGGGCCUCGGCUGU hsa-mir-2425 hsa-mir- 1307 gcggggGACCGAGAGGGCCUCGGCUGUgUgaggacUagagg
4523 cggccgaggcccgggccggUUcccccga
1014 UAGAGGCUGGAAUAGAGAUUCU hsa-mir-2426 pending 1308 aUUUUAGAGGCUGGAAUAGAGAUUCUUgaggcUUggaa
gagUaaggaUcccUUUaUcUgUccUcUaggag
1015 UAGCCUUCAGAUCUUGGUGUUU hsa-mir-2427 hsa-mir- 1309 UggaUcUgaaggcUgccccUUUgcUcUcUggggUAGCCUUCA
3614 GAUCUUGGUGUUUU
1016 CCACUUGGAUCUGAAGGCUGCC hsa-mir-2427* has-mir- 1310 UgggCCACUUGGAUCUGAAGGCUGCCccUUUgcUcUcUg
3614 gggUagccUUcagaUcUUggUgUUUU
1017 AUAGCAGCAUGAACCUGUCUCA hsa-mir-2428 hsa-mir- 1311 gaacgAUAGCAGCAUGAACCUGUCUCAcUgcagaaUUaUU
4524 UUgagacaggcUUaUgcUgcUaUccUUca
1018 UGAGACAGGCUUAUGCUGCUAU hsa-mir-2428* has-mir- 1312 ggaacgaUagcagcaUgaaccUgUcUcacUgcagaaUUaUUUU
4524 GAGACAGGCUUAUGCUGCUAUccUUca
1019 GGGGGGAUGUGCAUGCUGGUU hsa-mir-2429 hsa-mir- 1313 gUcagaGGGGGGAUGUGCAUGCUGGUUggggUgggcUgc
4525 cUgUggaccaaUcagcgUgcacUUccccacccUgaa
1020 CAGGGAGGUGAAUGGUUCUGUC hsa-mir-2430 pending 1314 cUUagcUcccUggcUUcagcccUUUUUcCAGGGAGGUGAA
UGGUUCUGUCUcgc
1021 GCUGACAGCAGGGCUGGCCGCU hsa-mir-2431 hsa-mir- 1315 UgcggUgacaUcagggcccagUcccUgcUgUcaUgccccaggUga
4526 cgUgcUggGCUGACAGCAGGGCUGGCCGCUaacgUcacUg
Uc
1022 UGGUCUGCAAAGAGAUGACUGU hsa-mir-2432 hsa-mir- 1316 ccagaagUGGUCUGCAAAGAGAUGACUGUgaaUccaagaU
4527 ccacaUcagcUcUgUgcUgccUacaUcUga
1023 UCAUUAUAUGUAUGAUCUGGAC hsa-mir-2433 hsa-mir- 1317 UaUUcUacUgagagUacagaUcUUUaUaUaUaUgaUcaUUa
4528 UaUgUaUgaUgagaUCAUUAUAUGUAUGAUCUGGACacc
cagUagaaUc
1024 AUUGGACUGCUGAUGGCCCGU hsa-mir-2434 hsa-mir- 1318 aUgacaggccaUcagcagUccaaUgaagacaUgaagacccaaUgU
4529 cUUcAUUGGACUGCUGAUGGCCCGUcacUggga
1025 UUGGAGGGUGUGGAAGACAUC hsa-mir-2435 pending 1319 cUccacaUUGGAGGGUGUGGAAGACAUCUgggccaacUcU
gaUcUcUUcaUcUaccccccaggacUggga
1026 CCCAGCAGGACGGGAGCG hsa-mir-2436 hsa-mir- 1320 cgaccgcacccgcccgaagcUgggUcaaggagCCCAGCAGGACG
4530 GGAGCGcggcgc
1027 AUGGAGAAGGCUUCUGA hsa-mir-2437 hsa-mir- 1321 gccUaggagUccUUggUcagUggggacAUGGAGAAGGCUUC
4531 UGAgga
1028 AAAAGCUGGGUUGAGAA hsa-mir-2438 hsa-mir- 1322 gccUUcUcUUcccagUUcUUccUggagUcggggAAAAGCUG
320e GGUUGAGAAggUgaaaaga
1029 UGGAAGGUAGACGGCCAGAGAG hsa-mir-2439 hsa-mir- 1323 ggUcaccUgUcUggccagcUacgUccccacggcccUUgUcagUg
3190 UGGAAGGUAGACGGCCAGAGAGgUgaccc
1030 UCUGGGAGGUUGUAGCAGUGGA hsa-mir-2440 hsa-mir- 1324 aggaagggaUUCUGGGAGGUUGUAGCAGUGGAaaaagUU
3192 cUUUUcUUccUcUgaUcgcccUcUcagcUcUUUccUUcUg
1031 CCCCGGGGAGCCCGGCG hsa-mir-2441 hsa-mir- 1325 acagaCCCCGGGGAGCCCGGCGgUgaagcUccUggUaUccU
4532 gggUgUcUga
1032 UGGAAGGAGGUUGCCGGACGCU hsa-mir-2442 hsa-mir- 1326 UgagaaUgUGGAAGGAGGUUGCCGGACGCUgcUggcUgc
4533 cUUccagcgUccacUUcccUUUcUcUcUcUcc
1033 CAAGAACCUCAAUUACCUUUGC hsa-mir-2443 pending 1327 cggUUggUgcaaaggUaaUUgUggUUUcUgccaUUgaaagUa
aaggCAAGAACCUCAAUUACCUUUGCagcgaccU
1034 AAUCUGAGAAGGCGCACAAGGU hsa-mir-2444 has-mir- 1328 UcgagggAAUCUGAGAAGGCGCACAAGGUUUgUgUccaa
3200 UacagUccacaccUUgcgcUacUcaggUcUgcUcgUg
1035 CACCUUGCGCUACUCAGGUCUG hsa-mir-2444* hsa-mir- 1329 UcgagggaaUcUgagaaggcgcacaaggUUUgUgUccaaUacag
3200 UccaCACCUUGCGCUACUCAGGUCUGcUcgU
1036 GGAGGAACCUUGGAGCUUCGGC hsa-mir-2445 hsa-mir- 1330 ggcUgaagcUcUaaggUUccgccUgcgggcaggaagcGGAGGA
3928 ACCUUGGAGCUUCGGCa
1037 GGAUGGAGGAGGGGUCU hsa-mir-2446 hsa-mir- 1331 UgUgaaUgacccccUUccagagccaaaaUcaccagGGAUGGAG
4534 GAGGGGUCUUgggUacU
1038 ACUGGACUAGGAGUCAGAAGG hsa-mir-2447 hsa-mir- 1332 gggagcACUGGACUAGGAGUCAGAAGGUggagUUcUggg
378i UgcUgUUUUcccacUcUUgggcccUgggcaUgUUcUg
1039 GUGGACCUGGCUGGGAC hsa-mir-2448 hsa-mir- 1333 aacUgggUcccagUcUUcacagUUggUUUcUgacacGUGGAC
4535 CUGGCUGGGACgaUgUg
1040 ACCUGGACCCAGCGUAGACAAA hsa-mir-2449 hsa-mir- 1334 ccACCUGGACCCAGCGUAGACAAAgaggUgUUUcUacUcc
3690 aUaUcUaccUggacccagUgU
1041 CAAAAACUGCAGUUACUUUUGU hsa-mir-2450 hsa-mir- 1335 agUUggUgcaaaagUaaUUgcggUUUUUgccgUcgaaaaUaa
548am UggCAAAAACUGCAGUUACUUUUGUaccaaUg
1042 UUGGGCUGGGCUGGGUUGGG hsa-mir-2451 hsa-mir- 1336 UUUGGGCUGGGCUGGGUUGGGcagUUcUUcUgcUggac
1587 UcaccUgUgaccagc
1043 UGUGGUAGAUAUAUGCACGAU hsa-mir-2452 hsa-mir- 1337 aUgUgUggUagaUaUaUgcacUgUaUaUaaacaUaaUGUGG
4536 UAGAUAUAUGCACGAUaUag
1044 AGAAGGGAAAGAACAUCAA hsa-mir-2453 pending 1338 aUggUgUUUgccUccUUcaUccgcaaggcaUcUgaUgcccacga
agUUaggaaggUccUUggggAGAAGGGAAAGAACAUCAAa
1045 CUGUCCUAAGGUUGUUGAGUU hsa-mir-2454 hsa-mir- 1339 cgcaUgacUcUUcaaccUcaggacUUgcagaaUUaaUggaaUg
676 CUGUCCUAAGGUUGUUGAGUUgUgca
1046 AAAAGGCAUUGUGGUUUUUG hsa-mir-2455 hsa-mir- 1340 caUUaggUUggUgcAAAAGGCAUUGUGGUUUUUGccUa
548an UaaaagUaaUggcaaaaaccgcaaUUccUUUUgcaccaaccUaa
U
1047 CGGGCGGCGGCUGUGUUGCGCA hsa-mir-2456 pending 1341 cacUcgcgcUgcggccagcgcccgggccUgcgggccCGGGCGGCG
GCUGUGUUGCGCAgUc
1048 UAUGGAAGGGAGAAGAGCUUUA hsa-mir-2457 hsa-mir- 1342 UUaUUaaUAUGGAAGGGAGAAGAGCUUUAaUgaUUgga
3202-1 gUcaUUUUcagagcaUUaaagcUcUUcUcccUUccaUaUUaa
UgU
1049 CGGCGGGGACGGCGAUUGGU hsa-mir-1908 hsa-mir- 1343 UgccgCGGCGGGGACGGCGAUUGGUccgUaUgUgUggUg
1908 ccaccggccgccggcUccgccccggcc
1050 CCUCCUGCCCUCCUUGCUGUAG hsa-mir-1976 pending 1344 gcaaagggUggcagcaaggaaggcaggggUccUaaggUgUgUCC
UCCUGCCCUCCUUGCUGUAGacUUUgg
1051 UGAGCCGAGCUGAGCUUAGCUG hsa-mir-2457 hsa-mir- 1345 UGAGCCGAGCUGAGCUUAGCUGggcUgagcUa
4537 accagggcUgggcUgagcUgggcUgagcUgagcUgagc
GAGCUUGGAUGAGCUGGGCUGAacUgggcUgg
1052 GAGCUUGGAUGAGCUGGGCUGA hsa-mir-2458 hsa-mir- 1346 gUUgagcUgggcUgggcUgagUUgagccaggcUgaU
4538 cUgggcUgag
1053 GCUGAACUGGGCUGAGCUGGGC hsa-mir-2459 hsa-mir- 1347 UgagcUgggcUcUgcUgUgcUgUgcUgagcagggcU
4539 gaGCUGAACUGGGCUGAGCUGGGC
1054 CUGGGCUGAAUGACAGUGAUGAG hsa-mir-2460 pending 1348 gUcaagUcagaacagccaggUagagcccUUgUccaaac
CUGGGCUGAAUGACAGUGAUGAG
1055 GGGAGCCGGGGCUGUGAGAGGA hsa-mir-2461 pending 1349 cUcUUUgagccUUggcUgccUUggUgcagcagggUc
aUcUgUagggccaccccacagcUcUUUccUUccccUc
cUcUcUccaGGGAGCCGGGGCUGUGAGAGGA
1056 UUAGUCCUGCCUGUAGGUUUA hsa-mir-2462 hsa-mir- 1350 aagcUgcaUggaccaggacUUggcaccUUUggccUU
4540 AGUCCUGCCUGUAGGUUUA
TABLE 33
Primers for Reverse Transcription for a subset of the known miRNAs
identified by deep sequencing analysis.
SEQ Putative Primer for reverse SEQ
ID Major/Minor Mature Sequence transcription ID
NO. miRNA ID Captured of miRNA NO.
7 hsa-mir-129- AAGCCCUUACCCCAAAAAGCAU AAGCCCTTACCCCAAAAAGCAT 1351
2*
22 hsa-mir-25* AGGCGGAGACUUGGGCAAUUG AGGCGGAGACTTGGGCAATTG 1352
24 hsa-mir-30b* CUGGGAGGUGGAUGUUUACUUC CTGGGAGGTGGATGTTTACTTC 1353
26 hsa-mir-30c- CUGGGAGAAGGCUGUUUACUCU CTGGGAGAAGGCTGTTTACTCT 1354
2*
27 hsa-mir-30e* CUUUCAGUCGGAUGUUUACAGC CTTTCAGTCGGATGTTTACAGC 1355
29 hsa-mir-342- UCUCACACAGAAAUCGCACCCGU TCTCACACAGAAATCGCACCCGT 1356
3p
56 hsa-let-7a-2 UGAGGUAGUAGGUUGUAUAGUU TGAGGTAGTAGGTTGTATAGTT 1357
57 hsa-let-7a-3 UGAGGUAGUAGGUUGUAUAGUU TGAGGTAGTAGGTTGTATAGTT 1358
58 hsa-let-7c UGAGGUAGUAGGUUGUAUGGUU TGAGGTAGTAGGTTGTATGGTT 1359
63 hsa-mir-100 AACCCGUAGAUCCGAACUUGUG AACCCGTAGATCCGAACTTGTG 1360
64 hsa-mir-101-1 UACAGUACUGUGAUAACUGAA TACAGTACTGTGATAACTGAA 1361
65 hsa-mir-101-2 GUACAGUACUGUGAUAACUGAA TACAGTACTGTGATAACTGAA 1362
66 hsa-mir-103-1 AGCAGCAUUGUACAGGGCUAUGA AGCAGCATTGTACAGGGCTATGA 1363
67 hsa-mir-103-2 AGCAGCAUUGUACAGGGCUAUGA AGCAGCATTGTACAGGGCTATGA 1364
70 hsa-mir-106a AAAAGUGCUUACAGUGCAGGUAG AAAAGTGCTTACAGTGCAGGTAG 1365
71 hsa-mir-106b UAAAGUGCUGACAGUGCAGAU TAAAGTGCTGACAGTGCAGAT 1366
72 hsa-mir-107 AGCAGCAUUGUACAGGGCUAUCA AGCAGCATTGTACAGGGCTATCA 1367
73 hsa-mir-10a UACCCUGUAGAUCCGAAUUUGUG TACCCTGTAGATCCGAATTTGTG 1368
74 hsa-mir-10b UACCCUGUAGAACCGAAUUUGUG TACCCTGTAGAACCGAATTTGTG 1369
98 hsa-mir-128-1 UCACAGUGAACCGGUCUCUUU TCACAGTGAACCGGTCTCTTT 1370
99 hsa-mir-128-2 UCACAGUGAACCGGUCUCUUU TCACAGTGAACCGGTCTCTTT 1371
100 hsa-mir-129-1 CUUUUUGCGGUCUGGGCUUGC CTTTTTGCGGTCTGGGCTTGC 1372
101 hsa-mir-129-2 CUUUUUGCGGUCUGGGCUUGC CTTTTTGCGGTCTGGGCTTGC 1373
103 hsa-mir-1295 UUAGGCCGCAGAUCUGGGUGA TTAGGCCGCAGATCTGGGTGA 1374
119 hsa-mir-140- UACCACAGGGUAGAACCACGG TACCACAGGGTAGAACCACGG 1375
3p
122 hsa-mir-143 UGAGAUGAAGCACUGUAGCUC TGAGATGAAGCACTGTAGCTC 1376
125 hsa-mir-146a UGAGAACUGAAUUCCAUGGGUU TGAGAACTGAATTCCATGGGTT 1377
126 hsa-mir-146b UGAGAACUGAAUUCCAUAGGCU TGAGAACTGAATTCCATAGGCT 1378
127 hsa-mir-148a UCAGUGCACUACAGAACUUUGU TCAGTGCACTACAGAACTTTGT 1379
130 hsa-mir-151- CUAGACUGAAGCUCCUUGAGG CTAGACTGAAGCTCCTTGAGG 1380
3p
131 hsa-mir-152 UCAGUGCAUGACAGAACUUGG TCAGTGCATGACAGAACTTGG 1381
132 hsa-mir-155 UUAAUGCUAAUCGUGAUAGGGGU TTAATGCTAATCGTGATAGGGGT 1382
133 hsa-mir-15a UAGCAGCACAUAAUGGUUUGUG TAGCAGCACATAATGGTTTGTG 1383
134 hsa-mir-15b UAGCAGCACAUCAUGGUUUACA TAGCAGCACATCATGGTTTACA 1384
135 hsa-mir-16-1 UAGCAGCACGUAAAUAUUGGCG TAGCAGCACGTAAATATTGGCG 1385
136 hsa-mir-16-2 UAGCAGCACGUAAAUAUUGGCG TAGCAGCACGTAAATATTGGCG 1386
137 hsa-mir-17 CAAAGUGCUUACAGUGCAGGUAG CAAAGTGCTTACAGTGCAGGTAG 1387
138 hsa-mir-181a- AACAUUCAACGCUGUCGGUGAGU AACATTCAACGCTGTCGGTGAGT 1388
1
139 hsa-mir-181a- AACAUUCAACGCUGUCGGUGAGU AACATTCAACGCTGTCGGTGAGT 1389
2
140 hsa-mir-181b- AACAUUCAUUGCUGUCGGUGGGU AACATTCATTGCTGTCGGTGGG 1390
1
141 hsa-mir-181b- AACAUUCAUUGCUGUCGGUGGGU AACATTCATTGCTGTCGGTGGG 1391
2
142 hsa-mir-181c AACAUUCAACCUGUCGGUGAGU AACATTCAACCTGTCGGTGAGT 1392
143 hsa-mir-181d AACAUUCAUUGUUGUCGGUGGGU AACATTCATTGTTGTCGGTGGGTT 1393
147 hsa-mir-185 UGGAGAGAAAGGCAGUUCCUGA TGGAGAGAAAGGCAGTTCCTGA 1394
150 hsa-mir-18a UAAGGUGCAUCUAGUGCAGAUAG TAAGGTGCATCTAGTGCAGATAG 1395
151 hsa-mir-191 CAACGGAAUCCCAAAAGCAGCUG CAACGGAATCCCAAAAGCAGCTG 1396
152 hsa-mir-192 CUGACCUAUGAAUUGACAGCC CTGACCTATGAATTGACAGCC 1397
153 hsa-mir-193a- UGGGUCUUUGCGGGCGAGAUGA TGGGTCTTTGCGGGCGAGATGA 1398
5p
159 hsa-mir-196b UAGGUAGUUUCCUGUUGUUGGG TAGGTAGTTTCCTGTTGTTGGG 1399
160 hsa-mir-197 UUCACCACCUUCUCCACCCAGC TTCACCACCTTCTCCACCCAGC 1400
163 hsa-mir-199b ACAGUAGUCUGCACAUUGGUUA ACAGTAGTCTGCACATTGGTTA 1401
164 hsa-mir-19a UGUGCAAAUCUAUGCAAAACUGA TGTGCAAATCTATGCAAAACTGA 1402
175 hsa-mir-20a UAAAGUGCUUAUAGUGCAGGUAG TAAAGTGCTTATAGTGCAGGTAG 1403
176 hsa-mir-20b CAAAGUGCUCAUAGUGCAGGUAG CAAAGTGCTCATAGTGCAGGTAG 1404
186 hsa-mir-221 AGCUACAUUGUCUGCUGGGUUUC AGCTACATTGTCTGCTGGGTTTC 1405
187 hsa-mir-222 AGCUACAUCUGGCUACUGGGU AGCTACATCTGGCTACTGGGT 1406
190 hsa-mir-23a AUCACAUUGCCAGGGAUUUCC ATCACATTGCCAGGGATTTCC 1407
194 hsa-mir-25 CAUUGCACUUGUCUCGGUCUGA CATTGCACTTGTCTCGGTCTGA 1408
195 hsa-mir-26a-1 UUCAAGUAAUCCAGGAUAGGCU TTCAAGTAATCCAGGATAGGCT 1409
196 hsa-mir-26a-2 UUCAAGUAAUCCAGGAUAGGCU TTCAAGTAATCCAGGATAGGCT 1410
197 hsa-mir-26b UUCAAGUAAUUCAGGAUAGGU TTCAAGTAATTCAGGATAGGT 1411
198 hsa-mir-27a UUCACAGUGGCUAAGUUCCGC TTCACAGTGGCTAAGTTCCGC 1412
203 hsa-mir-29a UAGCACCAUCUGAAAUCGGUUA TAGCACCATCTGAAATCGGTTA 1413
208 hsa-mir-30a UGUAAACAUCCUCGACUGGAAG TGTAAACATCCTCGACTGGAAG 1414
209 hsa-mir-30b UGUAAACAUCCUACACUCAGCU TGTAAACATCCTACACTCAGCT 1415
210 hsa-mir-30c-1 UGUAAACAUCCUACACUCUCAGC TGTAAACATCCTACACTCTCAGC 1416
211 hsa-mir-30c-2 UGUAAACAUCCUACACUCUCAGC TGTAAACATCCTACACTCTCAGC 1417
212 hsa-mir-30d UGUAAACAUCCCCGACUGGAAG TGTAAACATCCCCGACTGGAAG 1418
213 hsa-mir-30e UGUAAACAUCCUUGACUGGAAG TGTAAACATCCTTGACTGGAAG 1419
216 hsa-mir-320a AAAAGCUGGGUUGAGAGGGCGA AAAAGCTGGGTTGAGAGGGCGA 1420
217 hsa-mir-320b- AAAAGCUGGGUUGAGAGGGCAA AAAAGCTGGGTTGAGAGGGCAA 1421
1
218 hsa-mir-320b- AAAAGCUGGGUUGAGAGGGCAA AAAAGCTGGGTTGAGAGGGCAA 1422
2
227 hsa-mir-331 GCCCCUGGGCCUAUCCUAGAA GCCCCTGGGCCTATCCTAGAA 1423
231 hsa-mir-33a GUGCAUUGUAGUUGCAUUGCA GTGCATTGTAGTTGCATTGCA 1424
233 hsa-mir-340 UUAUAAAGCAAUGAGACUGAUU TTATAAAGCAATGAGACTGATT 1425
236 hsa-mir-34a UGGCAGUGUCUUAGCUGGUUGU TGGCAGTGTCTTAGCTGGTTGT 1426
239 hsa-mir-361- UUAUCAGAAUCUCCAGGGGUAC TTATCAGAATCTCCAGGGGTAC 1427
5p
241 hsa-mir-363 AAUUGCACGGUAUCCAUCUGUA AATTGCACGGTATCCATCTGTA 1428
242 hsa-mir-365-2 UAAUGCCCCUAAAAAUCCUUAU TAATGCCCCTAAAAATCCTTAT 1429
246 hsa-mir-374a UUAUAAUACAACCUGAUAAGUG TTATAATACAACCTGATAAGTG 1430
250 hsa-mir-378 ACUGGACUUGGAGUCAGAAGG ACTGGACTTGGAGTCAGAAGG 1431
259 hsa-mir-423 UGAGGGGCAGAGAGCGAGACUUU TGAGGGGCAGAGAGCGAGACTTT 1432
265 hsa-mir-449a UGGCAGUGUAUUGUUAGCUGGU TGGCAGTGTATTGTTAGCTGGT 1433
287 hsa-mir-503 UAGCAGCGGGAACAGUUCUGCAG TAGCAGCGGGAACAGTTCTGCAG 1434
296 hsa-mir-532 CAUGCCUUGAGUGUAGGACCGU CATGCCTTGAGTGTAGGACCGT 1435
316 hsa-mir-576- AAGAUGUGGAAAAAUUGGAAUC AAGATGTGGAAAAATTGGAATC 1436
3p
327 hsa-mir-625 AGGGGGAAAGUUCUAUAGUCC AGGGGGAAAGTTCTATAGTCC 1437
338 hsa-mir-7-1 UGGAAGACUAGUGAUUUUGUUGU TGGAAGACTAGTGATTTTGTTGT 1438
339 hsa-mir-7-2 UGGAAGACUAGUGAUUUUGUUGU TGGAAGACTAGTGATTTTGTTGT 1439
340 hsa-mir-7-3 UGGAAGACUAGUGAUUUUGUUGU TGGAAGACTAGTGATTTTGTTGT 1440
345 hsa-mir-874 CUGCCCUGGCCCGAGGGACCGA CTGCCCTGGCCCGAGGGACCGA 1441
351 hsa-mir-9-1 UCUUUGGUUAUCUAGCUGUAUGA TCTTTGGTTATCTAGCTGTATGA 1442
352 hsa-mir-9-2 UCUUUGGUUAUCUAGCUGUAUGA TCTTTGGTTATCTAGCTGTATGA 1443
353 hsa-mir-92a-1 UAUUGCACUUGUCCCGGCCUGU TATTGCACTTGTCCCGGCCTGT 1444
354 hsa-mir-92a-2 UAUUGCACUUGUCCCGGCCUGU TATTGCACTTGTCCCGGCCTGT 1445
356 hsa-mir-93 CAAAGUGCUGUUCGUGCAGGUAG CAAAGTGCTGTTCGTGCAGGTAG 1446
357 hsa-mir-9-3 UCUUUGGUUAUCUAGCUGUAUGA TCTTTGGTTATCTAGCTGTATGA 1447
364 hsa-mir-99a AACCCGUAGAUCCGAUCUUGUG AACCCGTAGATCCGATCTTGTG 1448
387 hsa-mir-570 AAAGGUAAUUGCAGUUUUUCCC AAAGGTAATTGCAGTTTTTCCCA 1449
TABLE 34
Primers for Reverse Transcription of a subset of novel miRNAs
identified by deep sequencing analysis.
SEQ Temporary Primer for Reverse SEQ
ID Assigned Mature Sequence Transcription ID
NO. miRNA ID Captured of miRNA NO.
773 hsa-miR- ACUGGACUUGGAGUCAGGA ACTGGACTTGGAGTCAGGA 1450
378c
774 hsa-mir-449c AGGCAGUGUAUUGCUAGCGGCU AGGCAGTGTATTGCTAGCGGCTGT 1451
775 hsa-mir-500- UGCACCCAGGCAAGGAUUCUGC TGCACCCAGGCAAGGATTCTGC 1452
2
784 hsa-mir-2224 UGAGGGAGGAGACUGCA TGAGGGAGGAGACTGCA 1453
785 hsa-mir-2225 ACUGGACUUGGAGCCAGAAG ACTGGACTTGGAGCCAGAAG 1454
786 hsa-mir-2226 GUCACUGAUGUCUGUAGCUGAG GTCACTGATGTCTGTAGCTGAGACGG 1455
787 hsa-mir-2227 GAUGAGGAUGGAUAGCAAGGAA GATGAGGATGGATAGCAAGGAAG 1456
789 hsa-mir-2229 AAAAGCAUCAGGAAGUACCCA AAAAGCATCAGGAAGTACCCA 1457
796 hsa-mir-2235 GUCAAAUGAAGGGCUGAUCACG GTCAAATGAAGGGCTGATCACGAAATA 1458
798 hsa-mir-2237 AGAGUUAACUCAAAAUGGACUA AGAGTTAACTCAAAATGGACTA 1459
799 hsa-mir-2238 UGUUGGGAUUCAGCAGGACCAU TGTTGGGATTCAGCAGGACCATT 1460
800 hsa-mir-2239 UAAAUAGAGUAGGCAAAGGACA TAAATAGAGTAGGCAAAGGACA 1461
805 hsa-mir-2244 AAGAGGAAGAAAUGGCUGGUUC AAGAGGAAGAAATGGCTGGTTCTCAG 1462
809 hsa-mir-2248 UAGUGGAUGAUGCACUCUGUGC TAGTGGATGATGCACTCTGTGC 1463
814 hsa-mir-2253 AAAGACUCUGCAAGAUGCCU AAAGACTCTGCAAGATGCCT 1464
816 hsa-mir-2255 AGGAGAAGUAAAGUAGAA AGGAGAAGTAAAGTAGAA 1465
817 hsa-mir-2256 AUGGCCAGAGCUCACACAGAGG ATGGCCAGAGCTCACACAGAGG 1466
819 hsa-mir-2258 AUCAGGGCUUGUGGAAUGGGAA ATCAGGGCTTGTGGAATGGGAAG 1467
820 hsa-mir-2259 AUGGCCAGAGCUCACACAGAGG ATGGCCAGAGCTCACACAGAGG 1468
821 hsa-mir-2260 UGAGGAUAUGGCAGGGAAGGGG TGAGGATATGGCAGGGAAGGGGA 1469
825 hsa-mir-2264 UGGGCUCAGGGUACAAAGGUU TGGGCTCAGGGTACAAAGGTTC 1470
828 hsa-mir- GCUGCACCGGAGACUGGGUAA GCTGCACCGGAGACTGGGTAA 1471
2266-1
829 hsa-mir- GCUGCACCGGAGACUGGGUAA GCTGCACCGGAGACTGGGTAA 1472
2266-2
836 hsa-mir-2272 UGUCGUGGGGCUUGCUGGCUUG TGTCGTGGGGCTTGCTGGCTTG 1473
838 hsa-mir-2274 ACUGGACUUGGAGGCAGAA ACTGGACTTGGAGGCAGAA 1474
841 hsa-mir-2277 UUGGAGGCGUGGGUUUU TTGGAGGCGTGGGTTTT 1475
844 hsa-mir-2280 CUGACUGAAUAGGUAGGGUCAU CTGACTGAATAGGTAGGGTCAT 1476
846 hsa-mir-2282 AGAUUGUUUCUUUUGCCGUGCA AGATTGTTTCTTTTGCCGTGCA 1477
847 hsa-mir- CACGGCAAAAGAAACAAUCCA CACGGCAAAAGAAACAATCCA 1478
2282*
848 hsa-mir-2283 CAGGGCUGGCAGUGACAUGGGU CAGGGCTGGCAGTGACATGGGT 1479
849 hsa-mir-2284 GGUGGGGGCUGUUGUUU GGTGGGGGCTGTTGTTT 1480
850 hsa-mir-2285 UGGGGAGGUGUGGAGUCAGCAU TGGGGAGGTGTGGAGTCAGCATG 1481
852 hsa-mir-2287 GGCUCCUUGGUCUAGGGGUA GGCTCCTTGGTCTAGGGGTA 1482
856 hsa-mir-2290 UGGGGAUUUGGAGAAGUGGUGA TGGGGATTTGGAGAAGTGGTGA 1483
857 hsa-mir-2291 AAAAGUGAUUGCAGUGUUUG AAAAGTGATTGCAGTGTTTGCC 1484
860 hsa-mir-2294 UAGGAGCUCAACAGAUGCCUGU TAGGAGCTCAACAGATGCCTGT 1485
861 hsa-mir-2295 AGCUUUUGGGAAUUCAGGUAG AGCTTTTGGGAATTCAGGTAG 1486
864 hsa-mir-2298 CAAAAGUGAUCGUGGUUUUUG CAAAAGTGATCGTGGTTTTTG 1487
865 hsa-mir-2299 AGGGUGUGUGUGUUUUU AGGGTGTGTGTGTTTTT 1488
871 hsa-mir-2305 ACUGACAGGAGAGCAUUUUGA ACTGACAGGAGAGCATTTTGA 1489
874 hsa-mir-2308 AUAGUGGUUGUGAAUUUACCUU ATAGTGGTTGTGAATTTACCTTC 1490
876 hsa-mir-2310 CUACCCCAGGAUGCCAGCAUAG CTACCCCAGGATGCCAGCATAGTT 1491
877 hsa-mir-2311 ACUGGACUUGGUGUCAGAUGG ACTGGACTTGGTGTCAGATGG 1492
880 hsa-mir-2314 UAGUGGAUGAUGGAGACUCGGU TAGTGGATGATGGAGACTCGGT 1493
888 hsa-mir-2322 AAGGUUUGGAUAGAUGCAAUA AAGGTTTGGATAGATGCAATA 1494
889 hsa-mir-2323 AAAGGUAAUUGCAGUUUUUCCC AAAGGTAATTGCAGTTTTTCCCA 1495
890 hsa-mir-2324 AGGGGACCAAAGAGAUAUAUAG AGGGGACCAAAGAGATATATAG 1496
896 hsa-mir-2328 GGCGACAAAACGAGACCCUGU GGCGACAAAACGAGACCCTGTC 1497
897 hsa-mir-2329 GGGUGCGGGCCGGCGGGG GGGTGCGGGCCGGCGGGGT 1498
899 hsa-mir-2331 CAUGCUAGGAUAGAAAGAAUGG CATGCTAGGATAGAAAGAATGG 1499
901 hsa-mir-2333 GCAAAGUGAUGAGUAAUACUGG GCAAAGTGATGAGTAATACTGG 1500
915 hsa-mir-2346 CCCUGGGGUUCUGAGGACAUG CCCTGGGGTTCTGAGGACATG 1501
917 hsa-mir-2348 CAGGAAGGAUUUAGGGACAGGC CAGGAAGGATTTAGGGACAGGC 1502
919 hsa-mir-2350 AUUAAGGACAUUUGUGAUUGAU ATTAAGGACATTTGTGATTGAT 1503
920 hsa-mir- AAAAGGCAUAAAACCAAGACA AAAAGGCATAAAACCAAGACA 1504
2351-1
921 hsa-mir- AAAAGGCAUAAAACCAAGACA AAAAGGCATAAAACCAAGACA 1505
2351-2
924 hsa-mir- UAAAAACUGCAAUUACUUUC TAAAAACTGCAATTACTTTC 1506
2354-1
926 hsa-mir- UGUGAUAUCAUGGUUCCUGGGA TGTGATATCATGGTTCCTGGGA 1507
2355-1
929 hsa-mir- UGUGAUAUCAUGGUUCCUGGGA TGTGATATCATGGTTCCTGGGA 1508
2355-2
931 hsa-mir- UGUGAUAUCAUGGUUCCUGGGA TGTGATATCATGGTTCCTGGGA 1509
2355-3
932 hsa-mir- UGUGAUAUCGUGCUUCCUGGGA TGTGATATCGTGCTTCCTGGGA 1510
2355b
936 hsa-mir-2358 AAAAGUAACUGCGGUUUUUGA AAAAGTAACTGCGGTTTTTGA 1511
938 hsa-mir-2360 GGAGUGGGCUGGUGGUU GGAGTGGGCTGGTGGTT 1512
940 hsa-mir- AAGGGCUUCCUCUCUGCAGGAC AAGGGCTTCCTCTCTGCAGGAC 1513
2362-1
941 hsa-mir- AAGGGCUUCCUCUCUGCAGGAC AAGGGCTTCCTCTCTGCAGGAC 1514
2362-2
949 hsa-mir-2370 AGAGCUGGCUGAAGGGCAG AGAGCTGGCTGAAGGGCAG 1515
953 hsa-mir-2374 UGUGACUUUAAGGGAAAUGGCG TGTGACTTTAAGGGAAATGGCG 1516
954 hsa-mir-2375 UCUCAGGAGUAAAGACAGAGUU TCTCAGGAGTAAAGACAGAGTT 1517
955 hsa-mir-2376 AGGUGGAUGCAAUGUGACCUCA AGGTGGATGCAATGTGACCTCA 1518
977 hsa-mir-2397 AGGCUGGGCUGGGACGGA AGGCTGGGCTGGGACGGA 1519
979 hsa-mir-2399 UAGGAUGGGGGUGAGAGGUG TAGGATGGGGGTGAGAGGTG 1520
988 hsa-mir-2406 UGAGGGAGUAGGAUGUAUGGUU TGAGGGAGTAGGATGTATGGTT 1521
991 hsa-mir-2409 AGACUGACGGCUGGAGGCCCAU AGACTGACGGCTGGAGGCCCAT 1522
994 hsa-mir-2412 UAGUGAGUUAGAGAUGCAGAGC TAGTGAGTTAGAGATGCAGAGC 1523
996 hsa-mir-2414 GGGAGAAGGGUCGGGGC GGGAGAAGGGTCGGGGC 1524
1002 hsa-mir-2416 CUCGUGGGCUCUGGCCACGGC CTCGTGGGCTCTGGCCACGGCC 1525
1003 hsa-mir- AGAAGGGGUGAAAUUUAAACGU AGAAGGGGTGAAATTTAAACGT 1526
2417-1
1004 hsa-mir- AGAAGGGGUGAAAUUUAAACGU AGAAGGGGTGAAATTTAAACGT 1527
2417-2
1005 hsa-mir- AGAAGGGGUGAAAUUUAAACGU AGAAGGGGTGAAATTTAAACGT 1528
2417-3
1007 hsa-mir-2419 GCUCAGGGAUGAUAACUGUGCUGAGA GCTCAGGGATGATAACTGTGCTGAGA 1529
1011 hsa-mir-2423 CUGGACUGAGCCAUGCUACUGG CTGGACTGAGCCATGCTACTGG 1530
1017 hsa-mir-2428 AUAGCAGCAUGAACCUGUCUCA ATAGCAGCATGAACCTGTCTCA 1531
1018 hsa-mir- UGAGACAGGCUUAUGCUGCUAU TGAGACAGGCTTATGCTGCTAT 1532
2428*
1022 hsa-mir-2432 UGGUCUGCAAAGAGAUGACUGU TGGTCTGCAAAGAGATGACTGTG 1533
1025 hsa-mir-2435 UUGGAGGGUGUGGAAGACAUC TTGGAGGGTGTGGAAGACATC 1534
1027 hsa-mir-2437 AUGGAGAAGGCUUCUGA ATGGAGAAGGCTTCTGA 1535
1028 hsa-mir-2438 AAAAGCUGGGUUGAGAA AAAAGCTGGGTTGAGAAG 1536
1029 hsa-mir-2439 UGGAAGGUAGACGGCCAGAGAG TGGAAGGTAGACGGCCAGAGAG 1537
1030 hsa-mir-2440 UCUGGGAGGUUGUAGCAGUGGA TCTGGGAGGTTGTAGCAGTGGA 1538
1036 hsa-mir-2445 GGAGGAACCUUGGAGCUUCGGC GGAGGAACCTTGGAGCTTCGGCA 1539
1038 hsa-mir-2447 ACUGGACUAGGAGUCAGAAGG ACTGGACTAGGAGTCAGAAGG 1540
1041 hsa-mir-2450 CAAAAACUGCAGUUACUUUUGU CAAAAACTGCAGTTACTTTTGT 1541
1046 hsa-mir-2455 AAAAGGCAUUGUGGUUUUUG AAAAGGCATTGTGGTTTTTG 1542
TABLE 35
miRNAs that differentiate ABC DLBCL vs.
GCB DLBCL
FoldChange pVal
ABC vs. ABC vs.
MicroRNA GCB GCB
UGGUCUGCAAAGAGAUGACUGUG −4.30138 0.001335
(SEQ ID NO. 1565)
CAAAAACUGCAGUUACUUUUGU −3.52085 0.022949
(SEQ ID NO. 1041)
UGGGGAUUUGGAGAAGUGGUGA −2.8374 0.000753
(SEQ ID NO. 856)
hsa-miR-129-3p −2.48708 7.39E−05
ACUGGACUUGGUGUCAGAUGG −1.3712 0.01173
(SEQ ID NO. 877)
hsa-miR-196b −1.28463 0.032606
hsa-miR-9 −1.00737 0.003176
hsa-miR-28-5p −0.82319 0.00026
hsa-miR-365 −0.78573 0.032749
hsa-miR-185 −0.75345 0.044082
hsa-miR-199b-3p −0.74898 0.015518
hsa-miR-152 −0.67388 0.001282
hsa-miR-23a −0.65979 0.028732
hsa-miR-193a-5p −0.54233 0.031154
hsa-miR-27a −0.49729 0.027207
hsa-miR-331-3p −0.47573 0.0128
hsa-miR-301a −0.45261 0.014231
hsa-miR-128 −0.4224 0.024065
AGAUUGUUUCUUUUGCCGUGCA 0.557435 0.02141
(SEQ ID NO. 846)
hsa-miR-625 0.747299 0.01813
hsa-miR-155 0.781901 0.024175
hsa-miR-20b 0.986918 0.000746
GGCUCCUUGGUCUAGGGGUA 1.945372 0.002596
(SEQ ID NO. 852)
UAGUGAGUUAGAGAUGCAGAGC 2.347695 0.009081
(SEQ ID NO. 994)
CAGGAAGGAUUUAGGGACAGGC 2.519326 0.04483
(SEQ ID NO.-917)
*The 3′-end G of SEQ ID NO. 1565 is optionally omitted (providing SEQ ID NO. 1022).
APPENDIX A
Average expression of the genes depicted in FIG. 6*
Naïve
vs Germinal Germinal
Germinal Center vs Center vs
Center Plasma Memory
Naïve GC GC PC GC
Aver- Aver- aver- Aver- Aver- Memory
ID Gene Name age age age age age Average
1 VPS37B 10.60 4.58 4.58 9.71
2 SCN3A 9.50 3.98
3 NT5E 10.76 5.69
4 TBC1D9 12.03 7.03 7.03 11.19 7.03 12.88
5 MAP7 1.64 8.03
6 SPRY1 10.40 5.72
7 TNFSF12 8.11 3.54 3.54 7.55
8 SOCS3 9.00 4.48 4.48 9.39
9 EDG1 11.43 6.91 6.91 8.69
10 CTGF 7.62 3.30 3.30 9.65
11 FAM46A 10.65 6.46 6.46 8.29
12 MOBKL2B 11.63 7.45
13 DNMT3A 10.56 6.44 6.44 7.85
14 BHLHB2 10.75 6.64 6.64 10.96
15 LAMC1 9.85 5.80 5.80 9.67
16 CD69 13.27 9.40 9.40 12.27
17 PLEKHA1 10.95 7.20
18 PTGDR 7.20 3.46
19 TXNIP 14.89 11.20 11.20 13.39 11.20 15.10
20 SIDT1 11.52 7.86 7.86 9.38
21 LY6E 8.57 4.92 4.92 7.39
22 IGF1R 8.73 5.13
23 PRICKLE1 11.68 8.09 8.09 9.69
24 CSDA 12.12 8.59 8.59 10.10
25 KLF2 12.61 9.10 9.10 11.70
26 ELOVL2 7.32 3.86
27 CCND2 12.06 8.61 8.61 11.53
28 LASS6 10.29 6.86 6.86 9.42 6.86 9.48
29 DUSP6 11.93 8.53 8.53 10.19
30 CUGBP2 14.28 10.93 10.93 14.29
31 PTPRO 9.64 6.32 6.32 8.63
32 PHF16 10.33 7.04
33 NR3C2 8.85 5.58
34 CRTC3 12.56 9.32 9.32 11.11
35 ADAMTS6 7.68 4.45
36 ETV6 10.36 7.14 7.14 8.89 7.14 10.43
37 LRRC17 3.10 7.49
38 FAM46C 12.18 8.98 8.98 14.76 8.98 10.54
39 SATB2 7.20 4.02 4.02 7.60
40 RNF125 9.00 5.83
41 ST6GALNAC3 8.12 4.96
42 LARGE 9.05 5.89 5.89 8.73
43 ZNF276 9.39 6.25
44 KCNA3 4.83 9.82
45 BCL2 10.89 7.76 7.76 11.41
46 MTSS1 12.92 9.82 9.82 12.05
47 NR6A1 9.10 6.03 6.03 7.62
48 BHLHB3 11.11 8.05 8.05 13.26 8.05 13.26
49 MYO10 7.22 4.19
50 ITM2C 6.44 13.85
51 C18orf1 9.49 6.47 6.47 8.63
52 FXYD7 7.36 4.35
53 DUSP8 7.96 4.96 4.96 8.41 4.96 7.75
54 BTBD3 7.41 4.42
55 TMEPAI 11.15 8.20 8.20 11.03
56 ANTXR2 9.90 6.97
57 FOSB 10.13 7.21 7.21 11.05
58 TMCC3 8.23 5.31 5.31 7.09 5.31 8.05
59 ARL4C 11.31 8.41 8.41 10.32
60 ZMYND11 11.33 8.43 8.43 11.34 8.43 11.68
61 RHOBTB1 7.33 4.48
62 JUN 12.14 9.34 9.34 12.70
63 SKI 10.46 7.67 7.67 10.68
64 TMEM121 7.07 4.29
65 IL13RA1 8.99 6.21
66 KIF13B 6.23 9.01
67 BHLHB5 4.26 7.39
68 PGM2L1 10.35 7.65
69 C14orf4 12.11 9.47 9.47 11.01 9.47 11.65
70 PDE4B 12.38 9.74 9.74 11.60
71 PDE7B 8.64 6.03
72 BCL9L 9.37 6.77 6.77 8.61
73 PCDH9 10.32 7.73
74 ARHGAP5 10.44 7.86 7.86 9.11 7.86 9.85
75 KIAA0802 9.18 6.61 6.61 7.91 6.61 7.89
76 ITGB4 7.48 4.91
77 NOTCH2NL 7.17 8.95
78 CDC42BPA 7.32 4.77
79 SPG20 9.25 6.73
80 KLF4 10.04 7.57 7.57 9.09
81 AHNAK 9.34 6.87 6.87 9.56 6.87 11.51
82 FCHSD1 9.15 6.69
83 KIAA1622 3.94 7.67
84 PLXNC1 10.92 8.48
85 TMEM150 8.39 5.96
86 ACVR2A 7.81 5.38 5.38 8.63 5.38 7.40
87 KALRN 5.61 7.64
88 ANK2 7.69 5.28 5.28 7.54 5.28 7.00
89 FLJ14213 7.61 5.21 5.21 7.97
90 CHPT1 10.92 8.52
91 TCF2 5.69 7.82
92 FGF5 5.41 7.52
93 SLC12A6 12.18 9.80 9.80 11.09
94 MGC17330 11.53 9.16
95 NR4A2 10.21 7.85 7.85 9.21 7.85 10.54
96 SLC39A10 11.06 8.70
97 LITAF 12.52 10.18 10.18 11.82
98 AKT3 9.32 6.99 6.99 9.25
99 PDCD4 12.89 10.57 10.57 13.33
100 STMN3 10.03 7.72
101 SIDT2 13.13 10.85
102 GRIA3 7.04 4.77
103 EML4 12.83 10.57
104 DIP2B 11.26 8.99 8.99 10.77
105 FBN1 4.89 8.17
106 FAM84B 9.21 6.96
107 EGR3 11.59 9.35 9.35 8.24 9.35 10.62
108 CTHRC1 4.25 11.94
109 RRAGD 7.69 5.46
110 MACF1 12.88 10.66
111 FOXF2 7.05 4.83
112 GAB1 8.01 5.81 5.81 10.91
113 ST18 4.42 7.40
114 ZFP36L2 13.78 11.59 11.59 13.43 11.59 14.05
115 GAB2 8.29 6.12 6.12 9.02
116 CHST11 11.17 8.99 8.99 10.28
117 CLOCK 9.85 7.68 7.68 9.91 7.68 9.62
118 PREX1 10.07 7.90 7.90 11.16
119 KLF11 8.08 5.94 5.94 8.39
120 PTGER4 11.66 9.53
121 KLF9 9.47 7.34 7.34 9.27
122 FLJ37078 7.55 5.42 5.42 7.49
123 ODZ2 7.20 5.08 5.08 7.25 5.08 7.22
124 SESN3 11.24 9.11 9.11 7.60
125 PDK4 7.22 5.09
126 CNTNAP3 7.35 5.23 5.23 7.10
127 DUSP1 13.34 11.22
128 MARCKS 11.19 9.07 9.07 11.05 9.07 12.21
129 SPRY4 7.02 4.91
130 LMO1 4.75 7.16
131 MTUS1 7.77 5.66 5.66 8.16
132 ADCY9 8.01 5.91 5.91 7.74
133 SLC17A6 3.80 7.06
134 NOX4 7.45 5.37 5.37 7.22
135 UTRN 9.46 10.97
136 ZBTB10 8.50 6.45
137 SLC26A7 8.37 6.32 6.32 7.89
138 PNRC1 13.14 11.09
139 LLGL2 6.83 9.29
140 CHST1 7.15 5.12
141 CREB3L2 10.53 8.51 8.51 13.30
142 DDIT4 12.69 10.68
143 C20orf108 8.82 6.80 6.80 10.19
144 CDH1 3.85 10.26
145 TFAP4 7.28 5.29
146 SLC38A2 13.57 11.58 11.58 13.74 11.58 13.17
147 SESN1 9.84 7.87 7.87 9.13
148 YPEL2 10.18 8.20 8.20 10.04
149 GRASP 8.47 6.50
150 TSC22D3 13.22 11.26 11.26 12.67
151 ATP11A 9.70 7.75 7.75 9.50
152 L3MBTL3 10.61 8.66 8.66 10.06
153 SORT1 7.61 5.67 5.67 8.11
154 CAV1 4.80 9.11
155 RXRA 9.07 7.15 7.15 8.81
156 CRELD1 8.40 6.48 6.48 8.26
157 RBMS1 12.40 10.49 10.49 8.80
158 LYST 10.90 8.99
159 PIP5K1B 9.61 7.70 7.70 9.97
160 JUNB 11.62 9.72
161 MBOAT1 4.80 7.27
162 IRF4 11.16 9.28 9.28 13.66
163 LIFR 4.62 7.08
164 MORC3 11.25 9.37
165 MBP 11.91 10.05 10.05 8.61
166 SRC 4.98 7.98
167 ALS2CR13 11.79 9.93 9.93 8.89 9.93 11.47
168 MYH1 4.40 7.37
169 DUSP3 9.36 7.50 7.50 9.16
170 HLX1 7.14 5.28 5.28 7.65
171 CDKN1A 9.09 7.23 7.23 8.97 7.23 8.43
172 SOCS5 10.00 8.15
173 PPP1R9A 5.14 7.35
174 TGFBR2 12.81 10.99 10.99 12.63
175 LRRC16 8.47 6.66
176 ZNF629 8.17 6.35 6.35 8.58
177 RPS6KA5 11.75 9.95 9.95 11.48
178 SATB1 12.79 10.99 10.99 9.06
179 SEMA4C 7.38 5.59 5.59 8.49 5.59 7.14
180 ULK1 7.79 5.99 5.99 8.72 5.99 8.06
181 STX3 8.88 7.09 7.09 8.91
182 BAMBI 4.81 7.26
183 MAP3K5 10.63 8.84 8.84 7.66 8.84 10.71
184 KIAA1147 11.70 9.91 9.91 11.53
185 SBK1 5.20 7.04
186 RYR3 7.80 6.02 6.02 8.27
187 ZNF238 13.05 11.27 11.27 10.08 11.27 12.54
188 IL12A 5.65 7.26
189 SLC2A3 12.00 10.23 10.23 11.89
190 GFPT2 3.39 7.18
191 G0S2 7.72 5.96
192 LYRM5 10.29 8.53 8.53 10.93
193 SSH2 11.67 9.91 9.91 11.85
194 NRP1 7.44 5.69 5.69 7.22
195 LMLN 8.92 7.19
196 UCP3 7.71 5.99
197 TMEM166 7.31 5.60
198 CACNA1I 7.46 5.76 5.76 7.06
199 PHOSPHO1 3.55 7.10
200 CRIM1 9.79 8.11 8.11 9.32
201 GATA6 5.41 7.59
202 SACS 10.06 8.39 8.39 10.54
203 CDKN1B 12.91 11.24 11.24 14.41
204 CACNA2D2 5.25 7.57 5.25 7.93
205 MKRN3 3.65 7.10
206 MTFR1 10.40 8.74 8.74 7.34 8.74 10.15
207 GALNT3 10.19 8.54
208 RPS6KA3 12.07 10.42 10.42 11.48
209 DTNA 5.47 7.86
210 MAGI2 7.04 5.40 5.40 7.42
211 FOXJ2 10.01 8.38 8.38 9.97 8.38 9.85
212 KIAA0513 5.29 8.44 5.29 7.36
213 NDRG1 7.75 10.02
214 AKAP7 10.16 8.54
215 CD72 13.74 12.12
216 IGFBP5 7.30 5.69 5.69 7.59
217 REPS2 7.51 5.92 5.92 7.60
218 PRDM12 7.17 5.57
219 ZNF3 8.86 7.27 7.27 8.75
220 TLL2 4.88 7.01
221 PCNX 9.77 8.19
222 ARHGAP24 11.31 9.73 9.73 8.65 9.73 11.32
223 THRAP2 11.31 9.73
224 RNF11 9.72 8.15 8.15 11.08
225 HOXC8 7.05 5.48
226 SCML2 10.33 8.76
227 BMPR2 9.70 8.14 8.14 10.06
228 STAC 5.27 7.20
229 C10orf54 9.12 7.56 7.56 10.16
230 FBXL17 9.51 7.95 7.95 9.36
231 CBX7 10.87 9.32 9.32 10.96
232 UBE2W 9.80 8.25 8.25 9.76 8.25 9.38
233 ProSAPiP1 4.12 7.54
234 UBL3 10.73 9.18 9.18 11.27 9.18 11.03
235 TTYH3 7.35 5.81 5.81 8.16
236 PUNC 5.39 8.85 5.39 7.30
237 GDF11 7.01 5.47 5.47 7.70
238 LMX1A 5.25 7.43
239 TIMP2 7.51 5.97 5.97 7.69 5.97 7.40
240 NHLH2 3.93 7.35
241 CLCF1 10.50 8.98
242 ITGB3 7.66 6.14
243 TMEM132E 3.93 7.20
244 KCTD17 7.45 5.94 5.94 7.33
245 ChGn 7.75 6.24 6.24 8.36
246 DIP2C 9.25 7.75
247 DKFZp667G2110 8.79 7.28 7.28 9.25
248 CSNK1G3 10.84 9.34 9.34 10.70
249 NRIP1 11.79 10.30
250 SMAD3 10.72 9.23 9.23 7.74 9.23 10.54
251 SHOX2 7.05 5.57
252 LDLRAP1 6.80 9.84 6.80 8.59
253 SUPT3H 7.77 6.30 6.30 8.05
254 KCNN2 7.18 5.70
255 DLL1 7.37 5.90
256 CEND1 7.72 6.26
257 NOTCH1 10.90 9.45
258 TLE1 7.74 9.63
259 FCHO2 8.75 7.30
260 JPH4 7.12 5.67 5.67 7.60
261 KCNMB2 7.16 5.72
262 KHDRBS2 7.17 5.73
263 LEFTY1 4.38 7.21
264 ST3GAL5 10.59 9.15
265 LGR4 5.30 7.29
266 FNDC8 4.25 8.01
267 NPTXR 5.87 7.65
268 PRICKLE2 5.47 7.47
269 GRIA4 5.19 7.04
270 RHOB 8.66 7.23 7.23 9.56
271 ZADH2 8.65 7.22
272 ZBTB41 9.73 8.31 8.31 9.42
273 GPRASP2 6.00 7.35
274 SYS1 10.79 9.36
275 RUNX1T1 7.37 5.95
276 DLX2 4.66 7.04
277 SLC30A7 11.58 10.17
278 PER1 8.82 7.40
279 NT5C3 12.67 11.25
280 PDE3A 11.62 10.21
281 OCRL 8.07 6.66 6.66 8.04
282 PSD3 7.99 6.60
283 LPHN1 7.80 6.41
284 TNRC6B 11.87 10.48 10.48 11.77
285 SNN 12.03 10.64 10.64 8.91 10.64 12.10
286 HERPUD2 11.56 10.18
287 UBQLNL 7.45 6.07
288 HES7 7.37 5.99
289 GALNT2 10.08 8.69 8.69 10.63
290 CAMKK1 8.45 7.07
291 ELN 5.44 7.18
292 ICK 8.09 6.72 6.72 8.01
293 POU4F2 7.09 5.72 5.72 7.21
294 GAS2 7.13 5.76 5.76 7.81
295 ARHGEF3 10.11 8.75 8.75 11.04
296 ZBTB4 11.45 10.09 10.09 11.78
297 CHD7 12.14 10.78 10.78 12.08
298 TMEM45B 5.97 7.61
299 CLDN11 5.74 7.22
300 PTPN1 10.85 9.51
301 PHF20 12.00 10.67
302 VAV3 10.44 7.84
303 CARKL 8.38 7.06 7.06 8.94
304 TRIM36 5.43 7.77
305 CTLA4 6.57 7.95
306 POLK 9.69 8.38 8.38 10.55
307 WSB1 13.22 11.91 11.91 13.02
308 ALS2CR2 9.87 8.57 8.57 10.14
309 PLK2 4.94 7.13
310 SRPK2 10.83 9.53 9.53 11.13
311 ARRDC2 10.86 9.55
312 IER5 13.10 11.80
313 EPN1 8.95 7.66 7.66 9.07
314 SLC20A2 9.45 8.16
315 DLG4 4.23 7.22
316 TMTC2 6.16 7.92
317 ETV1 8.19 6.90
318 JAZF1 11.93 10.65 10.65 7.49
319 VAV2 10.22 8.94
320 C15orf27 7.19 5.91
321 FYCO1 9.75 8.47
322 KIAA0789 7.27 6.00
323 OBFC2A 9.94 8.66 8.66 10.94
324 MCF2 7.19 5.92
325 KIAA2018 11.77 10.50 10.50 9.50
326 MTMR10 10.75 9.48
327 FAM63B 6.03 7.83 6.03 7.95
328 SNF1LK 10.20 8.93 8.93 10.18
329 ZNF385 8.89 7.63
330 SESTD1 11.05 9.78
331 SLC31A2 5.41 9.13
332 PCMTD1 12.03 10.76 10.76 12.44
333 NBEA 8.06 6.81 6.81 8.02 6.81 8.35
334 ZNF295 9.24 10.61
335 SIPA1L3 10.81 9.57
336 CC2D1B 9.11 7.87
337 PRKAG2 7.12 8.42
338 PKD1 9.04 7.81
339 CNTNAP2 7.54 6.32
340 FNBP1L 7.90 6.67
341 HEXIM1 10.27 9.05 9.05 10.24
342 C19orf2 12.29 11.07
343 MYLIP 11.62 10.41 10.41 11.81
344 SLC11A2 9.27 8.06
345 CLU 9.92 8.71
346 GCN5L2 10.18 8.97
347 DKFZP564J0863 10.85 9.65 9.65 11.11
348 CNNM2 7.85 6.64 6.64 8.07
349 CDC42SE1 12.07 10.87 10.87 12.18
350 HOXB9 6.20 8.41
351 NFATC3 11.39 10.19
352 UNC84B 11.12 9.93 9.93 8.59
353 DUSP5 6.23 9.44
354 ING1 11.35 10.17
355 ITM2B 11.23 13.62
356 FAM53B 11.67 10.49
357 ZFP36L1 14.07 12.89 12.89 10.09
358 NIPA1 9.75 8.57 8.57 10.07
359 GALNT1 10.93 9.76 9.76 11.12
360 MYPN 4.25 7.32
361 ITPK1 10.03 8.86
362 TTYH2 7.90 6.73
363 DOCK10 10.80 9.63 9.63 11.53
364 C14orf28 9.18 8.01 8.01 9.58 8.01 9.50
365 RP11-130N24.1 4.76 7.62
366 FGF12 5.82 7.33
367 ATP2B2 7.93 6.76
368 PPP1R9B 9.77 8.60
369 PPP3CA 13.50 12.33 12.33 13.86
370 TFAP2A 5.54 8.26
371 CYLD 10.91 9.75
372 PHF1 11.85 10.69 10.69 12.26
373 NEBL 7.98 6.82 6.82 8.08
374 ACIN1 12.04 10.89 10.89 11.99
375 SPTBN1 12.28 11.14 11.14 12.53 11.14 12.42
376 VAMP4 10.81 9.67 9.67 11.18
377 DNAJB9 10.80 9.66 9.66 15.21 9.66 11.03
378 ZDHHC2 11.55 10.42
379 TRIO 10.57 9.43
380 TMEM25 6.24 8.57
381 TAF9B 10.82 9.69
382 ARID3A 8.03 6.90 6.90 9.81
383 KIAA0182 10.68 9.55
384 RPS6KA2 8.61 7.49
385 C3orf58 11.47 10.34
386 CAST 11.94 10.82
387 SH3PXD2A 8.46 7.34
388 RAB6B 5.93 7.36
389 RNF141 11.47 10.35
390 SP4 11.26 10.14
391 ARMCX2 9.44 8.32 8.32 9.54
392 ZNF398 10.39 9.28
393 PBX3 9.69 8.58 8.58 7.00
394 FOS 12.35 11.25 11.25 13.34
395 FHOD3 6.40 7.94
396 C20orf59 10.11 9.01 9.01 10.92
397 FAM117A 11.67 10.57 10.57 12.06 10.57 11.64
398 ATP8B2 8.58 7.49 7.49 10.87 7.49 8.88
399 UXS1 9.53 8.43
400 GOLGA8A 12.84 11.74 11.74 13.14 11.74 13.05
401 SUFU 6.03 7.84
402 NAGPA 9.32 8.23
403 MLLT6 11.70 10.61
404 CPEB4 9.46 8.36 8.36 12.21
405 TMEM50B 10.67 9.58 9.58 11.41 9.58 10.63
406 AMPD3 11.65 10.56 10.56 8.76
407 SIX5 5.19 7.53
408 MMP16 5.66 7.47
409 LBH 11.87 10.79 10.79 8.83
410 POM121 9.60 11.26
411 ATP8B1 11.93 10.86 10.86 8.88
412 ARHGAP29 3.75 7.60
413 ZNF395 11.78 10.76
414 CRY2 6.50 8.01
415 BIN1 10.68 9.67
416 PSAP 11.24 13.41 11.24 12.71
417 ISL1 5.91 7.60
418 DNAJB5 8.04 6.97
419 KIAA0284 6.42 8.79
420 RBM35B 11.44 10.38
421 ADCY7 11.53 10.47
422 ARRDC3 9.46 10.68
423 MICAL1 11.72 10.67
424 MTHFR 10.31 9.26
425 HOOK3 8.57 7.53 7.53 6.52
426 EFNB3 5.16 7.79
427 ARHGAP12 9.86 8.82
428 LMBR1L 9.33 8.29
429 FGF7 5.88 7.86
430 USP2 5.50 7.52
431 SMARCA2 11.14 10.12
432 ELL2 8.23 13.60
433 C1orf26 7.06 10.01
434 CXCR4 14.61 13.45
435 HDGFRP3 5.58 7.39
436 BNC2 6.18 7.72
437 YPEL3 10.16 11.81 10.16 11.47
438 PPFIA3 4.58 8.06
439 DLGAP4 9.34 10.68
440 ZBTB7A 9.84 11.10
441 D4S234E 5.90 7.38
442 FNDC3A 9.41 13.60
443 FOXC1 5.84 7.39
444 KIF26B 5.55 7.30
445 NAV2 6.26 7.90
446 DDX3Y 8.07 10.38
447 FGD1 7.31 6.06
448 HTR4 7.26 9.01 7.26 8.40
449 C22orf31 5.67 8.10
450 RNF44 11.48 10.14
451 GALNAC4S-6ST 10.43 12.71
452 CCNT2 10.15 11.23
453 CHIC1 8.91 10.02
454 NNAT 5.97 8.19 5.97 7.84
455 CTDP1 6.81 8.26
456 BTG1 14.95 13.94
457 CMTM4 5.27 7.81
458 GOLGA8B 11.37 12.63
459 HBP1 10.07 12.13
460 GDPD1 6.35 7.61
461 CDYL 9.31 10.61 9.31 10.50
462 ZNF217 10.77 9.73
463 KIF5A 6.13 7.75
464 C1QL1 5.80 8.11
465 SOLH 7.88 9.03
466 ZC3H6 9.81 10.96
467 LATS2 7.61 10.19
468 COL18A1 6.46 7.85
469 C11orf24 9.89 11.07
470 SCUBE3 6.14 7.88
471 SEMA4G 5.57 7.81
472 FOXP1 12.31 10.84
473 TRIB2 10.62 9.61
474 LOC285382 5.35 7.20
475 RUNX2 7.91 10.22
476 LOC196463 4.16 7.52
477 LPGAT1 10.57 7.99
478 RASSF2 12.52 10.93
479 IRF1 9.86 11.60
480 RAB40B 7.31 10.89
481 CTDSPL 6.37 7.83
482 CLCN4 7.44 6.39
483 CACNB1 6.41 8.20
484 SYNGR1 8.45 10.40
485 ST8SLA4 10.67 11.81
486 PLD3 7.63 10.59
487 FOXO3A 10.17 12.41
488 TSPAN33 10.49 9.38
489 HIST1H4F 6.20 8.08
490 LAPTM4A 12.05 13.06
491 PRKCB1 12.14 10.50
492 PROX1 6.38 7.89
493 CDK5R1 10.12 7.78
494 MAP3K9 7.96 9.13
495 GPX3 5.92 7.61
496 GNS 8.79 11.06
497 ARL15 8.27 6.51
498 OXR1 9.21 11.17 9.21 10.25
499 AAK1 7.38 9.54 7.38 8.47
500 SH3PX3 6.72 8.58
501 MS4A7 10.55 8.35
502 FLJ20273 5.96 11.23 5.96 8.65
503 ISCU 12.01 13.55
504 ITGA2 5.70 7.60
505 ME1 5.87 7.89
506 LRP1 4.92 7.04
507 ZNF652 9.66 10.81
508 TRAK1 11.07 9.85
509 SLC8A1 6.27 7.83
510 C1orf119 10.76 11.98
511 KLF6 11.33 9.24 11.33 12.40
512 TRIM2 6.22 8.20
513 USP3 11.11 12.51
514 ARID5B 11.02 12.48
515 RASD1 6.15 9.32
516 ZCCHC2 10.63 8.93
517 LEFTY2 5.08 7.03
518 BACH1 9.97 11.76
519 IRAK1 11.48 12.80
520 RP11-217H1.1 10.76 13.04
521 HLCS 6.54 8.45
522 NAGK 10.94 12.14
523 CELSR2 6.26 7.89
524 PCBP4 7.75 9.08
525 FLJ25476 9.49 10.72
526 TPP1 12.10 11.06
527 ACVR1 7.95 9.31
528 EHD3 8.99 10.35
529 FAM80B 9.35 8.02
530 SPRYD3 8.19 9.51
531 PRDM4 9.81 10.83
532 C6orf134 7.92 5.60
533 HSP90B1 12.04 16.36
534 DYNC1I1 5.98 7.33
535 NFLX 7.43 8.67
536 DOCK4 5.96 7.97
537 ZNF287 7.09 8.47
538 XRN1 10.92 12.37
539 YES1 6.39 8.60
540 RBM35A 8.19 9.44
541 HOXB4 7.58 9.27
542 OTUD7B 7.24 8.57
543 CELSR3 6.83 8.39
544 RHOC 7.71 8.84
545 ZNF607 8.10 6.95
546 XYLT1 10.28 8.78 10.28 8.86
547 FAM89B 9.16 10.75
548 OSBPL8 12.99 11.66
549 SRCRB4D 6.82 8.67
550 RASL12 5.05 8.07
551 NAG8 8.39 9.78
552 MAN1A2 10.83 12.15
553 PPARA 8.59 9.65
554 CLDN12 6.66 8.39
555 ID4 5.93 7.79
556 HECTD2 7.98 6.80
557 EFCAB4A 5.93 8.96
558 CREBL1 6.97 8.09
559 SARM1 6.98 8.29
560 MIDN 8.48 7.29
561 EMB 10.48 8.65
562 FOXK2 8.40 9.63
563 INADL 6.87 8.44
564 KCNAB1 6.26 9.76
565 MAP3K8 10.92 9.60
566 C8orf58 7.89 5.62
567 SLC4A7 9.82 8.52 9.82 10.90
568 RAB8B 12.46 10.92
569 C10orf118 8.93 10.20
570 CRTC2 9.37 7.94
571 KLC2 7.31 9.19
572 SRCAP 8.06 6.96
573 CNTFR 7.15 8.67
574 SPTBN2 4.75 7.11
575 SMAD7 8.69 9.93
576 NDFIP1 9.10 10.95
577 BCL11A 14.03 10.63
578 SFXN5 7.26 8.87
579 RIMBP2 5.93 8.33
580 FUT8 8.62 11.08
581 PSEN2 5.86 9.17
582 MAP6 5.22 7.37
583 FOXO1A 11.53 10.14
584 BTG2 11.81 13.82
585 C10orf56 6.93 9.30
586 MAPK1 12.06 10.36
587 ZBTB47 6.63 8.12
588 GOSR2 9.73 11.07
589 ZFP90 10.15 8.92
590 RALGPS1 8.02 9.38
591 DGKI 4.77 7.39
592 RGL2 11.08 9.55
593 PAK6 5.90 8.55
594 DMXL1 11.85 10.31
595 TMEM113 12.28 11.03
596 SNX24 6.75 8.53
597 HOXA3 5.99 8.27
598 SAMD4A 9.84 8.36
599 WDR45 9.88 12.43
600 TLOC1 12.91 14.52
601 LARP2 7.64 11.50
602 DTX2 7.60 6.20
603 ITGA9 7.53 6.32
604 FASTK 10.04 11.22
605 TAOK2 7.47 6.37
606 CRTAP 9.78 11.08
607 SERTAD2 11.91 10.82
608 TBP 10.14 9.09
609 IQGAP2 7.26 11.19 7.26 8.93
610 RASGEF1B 6.23 8.01
611 PIP5K2B 9.69 8.53
612 PRRC1 10.32 11.92
613 RHPN2 6.73 8.21
614 DYRK1B 5.92 7.61
615 ADCY2 5.98 7.97
616 C12orf34 5.61 7.09
617 GMPR 5.35 8.15
618 PAK2 10.86 9.66
619 KIAA1539 8.12 9.49
620 PLAGL1 10.03 6.68
621 LCORL 9.74 11.29
622 BCL9 7.21 9.16
623 CPEB2 9.09 10.23
624 TMEM59 12.10 14.75
625 TRIP10 7.17 9.36
626 CCPG1 8.38 11.86
627 NDE1 10.71 9.30
628 ZDHHC21 9.38 8.11
629 STOX2 5.87 7.66
630 RAB4B 10.63 8.66
631 LRRFIP1 13.25 11.99
632 OSBP 10.10 11.39
633 RAB6A 10.76 12.27
634 RHOV 4.88 7.19
635 SLC39A14 8.80 11.00
636 ADARB1 10.05 7.69
637 ESPN 6.85 8.19
638 SEC31A 11.64 13.61
639 PJA2 10.85 11.99
640 SYNGR3 9.01 7.56
641 LOC339745 11.94 10.54
642 NEDD9 11.23 12.48
643 COL23A1 5.41 7.02
644 PIP5K3 11.93 10.79
645 PTPRG 7.30 8.49
646 DAB2IP 5.61 8.30
647 MAPRE3 5.80 8.60
648 SQSTM1 10.05 11.45
649 RAB6C 10.88 12.04
650 FAM57A 7.58 6.18
651 YPEL5 12.41 13.78
652 TTN 9.61 7.03
653 HTRA3 5.62 8.02
654 CHST2 7.80 12.49
655 BAZ1A 11.43 10.06
656 LRFN5 7.38 6.21
657 MBNL1 14.10 12.62
658 MLL2 10.93 9.89
659 SF1 11.44 10.36
660 FRMPD1 4.80 7.06
661 PPP2R5B 7.60 9.12
662 RNF43 10.04 8.01
663 GAP43 5.07 7.49
664 NOMO1 11.11 14.31
665 VEGFA 7.79 9.36
666 C22orf5 8.75 11.38
667 AKAP11 11.79 10.64
668 INSIG2 9.46 11.32
669 PDIK1L 9.11 10.72
670 TMEM4 10.93 13.32
671 LMNA 7.80 9.75
672 TP53INP1 11.84 14.14
673 NAV1 6.96 8.31
674 SPTY2D1 9.25 10.77
675 CREBL2 9.95 11.10
676 MFAP3L 5.90 7.46
677 REXO2 10.96 13.71
678 SPTBN4 6.77 8.22
679 NOMO2 9.74 12.94
680 PTEN 12.78 11.40
681 FBXL16 6.86 9.56
682 GPM6A 7.87 6.15 7.87 6.67
683 PRX 6.76 8.91
684 HOXC11 6.40 7.88
685 KIAA0329 8.27 9.87
686 PAFAH1B1 11.68 10.33
687 C20orf174 9.58 7.27
688 MTPN 13.87 12.17
689 TMED5 12.25 14.41
690 FURIN 8.03 9.54
691 ELAVL3 4.66 8.01
692 SH2B3 10.31 8.87
693 LIN28B 5.99 7.58
694 KIAA1033 12.65 11.38
695 TMEM28 4.95 7.14
696 TBC1D15 10.30 11.52
697 GOLGA4 10.11 11.71
698 STX1A 5.63 7.82
699 SLC40A1 6.87 8.27
700 KIAA1815 9.18 7.93
701 HNT 5.53 7.09
702 PDE11A 5.72 7.82
703 TGFBR3 7.84 9.52
704 MTF1 9.92 8.86
705 MAN1A1 9.99 13.65
706 THRA 7.26 8.48
707 CBLB 11.46 9.08
708 SLC35F1 6.29 8.16
709 FOXP4 8.52 7.30
710 ELOVL5 13.83 12.78
711 NMUR1 4.13 7.31
712 ITPR1 12.06 9.69
713 CDCA7L 11.95 10.33
714 RASSF1 9.35 10.69
715 IL1RAPL1 6.42 8.18
716 AUH 9.37 11.19
717 DIAPH1 11.52 9.74
718 ENSA 12.33 11.22
719 FAM43A 10.62 9.39
720 CSMD3 4.79 7.58
721 SLC25A37 10.10 8.69
722 SLC33A1 9.78 12.19
723 CNOT6 9.98 8.93
724 GRID1 7.05 8.80
725 C21orf91 11.51 10.18
726 ITPKB 11.28 8.91
727 CP 5.89 7.41
728 HMBOX1 10.05 8.50
729 ARID1A 12.22 11.11
730 C5orf5 11.86 10.38
731 TMSB4X 16.86 15.10
732 EPAS1 7.02 8.39
733 HPS3 11.65 10.16
734 DKK1 6.16 7.86
735 ZNF282 7.89 5.40
736 AP3D1 11.29 12.84
737 DERL1 11.69 13.43
738 C5orf13 9.89 7.62
739 CCNG2 13.32 11.64
740 PGRMC2 9.44 11.38
741 ARF6 13.60 12.55
742 TMEM110 7.75 9.24
743 FAM13A1 7.11 9.69
744 BRMS1L 8.58 10.18
745 TRAM1 12.52 15.17
746 CALU 9.42 11.20
747 GOLPH3 11.55 12.81
748 MAP3K7 11.14 10.06
749 ABCG4 6.90 8.22
750 NELF 8.75 7.70
751 ADIPOR1 9.67 10.92
752 INSR 8.48 11.62
753 GRM5 6.24 7.65
754 TFG 10.76 12.30
755 USP48 11.91 13.09
756 CLCN6 9.26 7.48
757 ZNF219 6.11 8.42
758 C7orf43 8.34 6.62
759 NFKB1 11.90 10.70
760 ARF3 11.11 9.87
761 JMJD1C 12.34 11.11
762 ROD1 13.13 11.42
763 OXSR1 10.69 9.15
764 ERGIC2 10.86 12.81
765 EIF4E3 7.61 9.87
766 SEC24A 10.05 12.85
767 SPRED1 7.19 5.77
768 HNRPH3 12.60 11.38
769 ZDHHC7 10.34 9.05
770 HNRPA1 16.14 15.14
771 TMED2 12.01 14.26
772 VASP 10.60 8.60
773 CHD4 11.40 10.31
774 RTN4RL1 5.48 8.36
775 PPP1R12A 12.03 10.97
776 SGK3 9.58 11.68
777 ARHGAP17 12.37 8.62
778 GORASP2 11.28 13.30
779 C2orf44 8.38 6.72
780 FAM116A 11.12 10.08
781 SSR1 12.75 14.73
782 MYH9 11.24 9.89
783 PIK3CD 12.99 10.03
784 DAZAP2 14.26 13.23
785 SEL1L 10.74 14.04
786 LOC388284 7.05 8.32
787 THRB 7.51 6.11 7.51 6.29
788 MGAT2 11.91 14.44
789 C8orf13 9.35 6.47
790 PDZD2 7.00 4.39
791 DERL2 10.74 12.75
792 ZCCHC5 7.39 6.27
793 NFYC 10.27 9.03
794 GLUD2 8.88 7.58
795 ESR1 7.55 9.88
796 NRP2 6.51 8.13
797 NUS1 10.01 11.53
798 BIRC6 12.15 10.94
799 ARID3B 7.83 9.22
800 STCH 10.16 13.88
801 SLC7A11 6.32 8.00
802 OSBPL5 7.91 9.22 7.91 6.62
803 IGFBP3 5.60 7.61
804 LNPEP 13.72 11.05
805 ZNRF1 8.18 9.68
806 DDAH1 7.96 5.87
807 ERG 4.07 7.49
808 APLP2 10.90 8.38
809 ZNFX1 10.14 8.93
810 GPM6B 8.66 6.69
811 BLCAP 11.49 10.10
812 SRP68 11.47 12.88
813 PIK3AP1 14.12 11.74
814 ANKRD28 11.00 12.46
815 KCNH8 8.27 4.80
816 KIAA0430 12.43 10.61
817 PPP2R1B 9.84 8.41
818 TMED9 11.62 13.82
819 CTNNBIP1 7.31 8.89
820 PPP2R5D 9.51 7.92
821 CBX4 12.36 13.69
822 AKAP6 7.38 5.55 7.38 4.93
823 C2orf30 10.72 13.84
824 DR1 11.97 10.65
825 MTDH 12.46 13.96
826 ETS1 14.40 12.62
827 EPHA8 6.12 8.10
828 ANKRD13B 7.23 5.85
829 C4orf16 8.83 7.70
830 SOX4 9.14 10.20
831 SLC12A2 10.23 9.01
832 IER3IP1 11.35 12.56
833 DST 7.02 8.34
834 WRNIP1 11.03 9.49
835 CLCC1 9.63 11.48
836 B4GALT6 7.90 6.82
837 BTBD10 10.12 8.07
838 SDC1 7.05 12.14
839 C10orf12 10.32 8.40
840 ZDHHC3 9.80 8.78
841 GNAI2 10.86 9.27
842 HNRPU 13.82 12.47
843 GNAZ 8.86 7.26
844 ALG9 8.93 10.97
845 FBXL10 11.75 10.43
846 ARID4B 12.19 10.96
847 PPP1R3F 6.19 7.79
848 EPB49 6.49 7.94
849 PCNP 12.91 11.74
850 PCYT1B 11.35 9.77
851 RAB14 11.64 10.41
852 TCERG1 12.50 11.46
853 MKNK2 14.87 12.67
854 COL4A3 8.78 12.23
855 HNRPA0 12.50 11.36
856 P2RX4 8.88 10.29
857 JUP 9.23 5.70
858 EGR2 10.43 8.07
859 SUPT16H 11.35 9.60
860 PNRC2 13.93 11.64
861 SRPR 11.87 13.89
862 BPTF 12.46 11.34
863 RBM16 12.05 10.52
864 YWHAZ 13.62 12.61
865 EDEM3 11.01 12.76
866 C12orf23 11.18 12.40
867 DOC2A 3.96 7.21
868 SETD2 11.54 10.39
869 FAM98A 10.91 12.10
870 C13orf18 13.25 9.86
871 BRP44L 10.48 11.91
872 CCDC6 11.25 9.82
873 GLCCI1 12.22 15.19
874 PLEKHH1 7.03 9.25
875 SMEK1 11.38 10.37
876 ANKRD9 4.70 8.61
877 CSNK1E 8.86 11.48
878 DCUN1D3 7.48 5.95
879 LTB 14.66 9.91
880 EOMES 7.30 6.14
881 PARP8 10.81 8.69
882 ABCD1 7.12 8.49
883 HOXA10 5.56 7.32
884 RNPS1 12.60 11.25
885 AYTL2 12.08 10.79
886 FLJ20699 5.74 8.80
887 SAPS1 11.43 9.76
888 SYNJ1 8.36 9.64
889 ZNF403 12.06 10.91
890 KPNB1 13.37 12.26
891 ISOC1 9.67 10.98
892 HYOU1 9.95 11.85
893 ZNF706 12.83 14.30
894 HDLBP 10.60 13.37
895 ELF1 12.70 11.34
896 TM9SF4 8.66 10.55
897 RAB8A 11.94 10.88
898 MORF4L2 12.94 14.69
899 SERP1 13.31 15.16
900 ADAMTS5 5.71 7.31
901 TRIOBP 11.23 10.18
902 ZNF664 11.32 12.57
903 RAB11FIP1 11.78 9.03
904 MYO18A 8.06 6.19
905 DUSP10 10.13 8.70
906 ATXN1 6.66 10.65 6.66 10.25
907 EPB41L4B 6.12 7.95
908 CNTNAP1 6.51 9.06
909 SLC17A7 6.47 8.01
910 SPCS2 13.75 15.53
911 CCNC 12.68 14.10
912 WDR77 10.67 8.83
913 GMFB 12.03 10.70
914 ITGA6 6.72 10.51
915 CASP8AP2 10.55 9.44
916 SEC24D 8.79 11.88
917 CBX1 12.16 10.96
918 TBX3 6.05 7.89
919 NFAT5 12.09 10.72
920 ATP6V1B2 10.73 9.53
921 ACTG1 16.72 14.81
922 RND3 7.31 5.65
923 HNRPUL1 11.97 10.58
924 TJP1 7.18 10.59
925 RAPGEF4 5.25 8.09
926 RGMA 5.65 7.42
927 NAP1L1 14.70 12.60
928 TARDBP 12.72 11.49
929 DAZAP1 12.74 11.30
930 ZNF609 8.26 6.94 8.26 9.52
931 ARL1 10.44 12.41
932 ERC2 7.03 5.97
933 PDE4A 7.65 9.22
934 CSK 12.83 10.59
935 IMPAD1 8.44 10.05
936 PNN 13.75 12.48
937 DCP2 13.06 10.74
938 PIK3R3 6.34 7.82
939 RAB1A 13.59 14.83
940 CENTB2 12.39 10.97
941 SPTAN1 11.48 9.79
942 DNAJC8 12.25 11.00
943 UNC13B 6.70 8.35
944 DHX15 14.19 12.99
945 VEZF1 11.45 9.94
946 DHDDS 8.74 10.05
947 FAM55C 8.19 9.74
948 USP37 8.89 7.60
949 MMD 11.68 10.32
950 HCLS1 14.42 13.12
951 KIAA1370 13.30 14.72
952 GANC 8.31 6.12
953 SSRP1 11.34 10.27
954 G3BP1 12.24 10.55
955 BAAT 4.91 7.24
956 FNDC3B 8.27 14.34
957 FBXW7 10.21 12.19
958 SPECC1L 10.27 7.66
959 YBX1 15.31 14.20
960 YAP1 7.23 5.61
961 ARF4 12.09 14.23
962 PNKD 10.86 9.79
963 CLEC2D 11.96 10.90
964 XPO1 13.81 11.82
965 TRAM2 9.38 12.80
966 MITF 8.05 6.41
967 CFL1 16.37 14.09
968 CREB5 5.46 7.24
969 GREM1 4.48 7.14
970 LONRF1 11.26 10.00
971 NME7 9.37 8.30
972 PMAIP1 12.26 10.75
973 KLHL14 11.89 10.84
974 AOF1 9.33 7.96 9.33 8.28
975 SMPD3 5.90 7.83
976 RGL1 6.66 8.04
977 LYPD6 5.38 7.39
978 STAT5B 10.95 9.73
979 C10orf6 10.04 8.44
980 CLDND1 10.72 12.01
981 DUSP9 5.59 7.70
982 SLC36A1 8.53 7.23
983 PAM 8.90 10.86
984 GHR 5.87 7.56
985 CBL 11.69 10.26
986 CENTG2 5.96 7.39
987 BACH2 13.68 9.35 13.68 11.88
988 NUP153 12.33 10.55
989 CPD 8.37 9.72
990 APLN 8.58 6.47
991 RFXDC2 11.67 10.00
992 GABRB2 5.58 8.10
993 NOMO3 5.39 7.39
994 RHOJ 6.41 7.75
995 PRDM1 9.15 14.10
996 IGSF3 7.62 5.91
997 UBE2A 12.47 11.47
998 KBTBD8 12.82 9.73 12.82 11.71
999 PRR15 7.27 5.94 7.27 4.67
1000 ILF3 11.94 10.71
1001 WIPF1 13.81 12.41
1002 GCNT2 11.01 8.49
1003 DBNL 10.43 8.48
1004 C14orf43 10.14 8.01
1005 MID1IP1 9.16 8.11
1006 LIMD2 10.43 8.88
1007 HNRPK 14.49 12.95 14.49 13.46
1008 ZNF697 5.74 8.07
1009 TSHZ3 7.31 5.32
1010 MBNL2 11.88 13.47
1011 ELL 7.83 9.17
1012 MAFB 7.30 8.56
1013 GGA2 13.25 10.77
1014 C20orf121 10.01 8.93
1015 CDC2L6 11.52 9.69
1016 TOB2 11.39 9.58
1017 MAP4K4 12.04 10.41
1018 FAM102A 11.41 10.18
1019 ITGB3BP 11.86 10.76
1020 HIVEP1 10.35 9.05
1021 NUDT21 12.51 11.11
1022 EIF4A1 14.02 12.98
1023 TSPAN14 8.38 6.31
1024 SLC2A4RG 8.98 7.40
1025 DGKZ 9.74 7.85
1026 PTPN9 9.30 7.85
1027 BCL7A 11.33 10.10
1028 LSM14A 12.74 11.19
1029 GNB2 11.50 10.12
1030 SLC6A6 11.35 8.51
1031 TACC1 13.21 11.62
1032 LIMK2 9.80 8.12
1033 ACVR2B 6.69 8.33
1034 SCC-112 12.25 10.55
1035 PTBP1 13.38 12.19 13.38 12.21
1036 CITED2 9.88 12.56
1037 SNX5 14.17 13.16
1038 ACTR2 15.12 13.32
1039 OSBPL3 10.59 12.84
1040 SFRS1 14.60 13.24
1041 ADM 7.24 11.10
1042 PFKFB3 11.91 10.01
1043 FNBP1 13.61 12.25
1044 MTMR2 10.53 9.06
1045 DDHD1 10.18 8.74
1046 HSPA5 13.70 16.32
1047 PTK2 12.12 8.33
1048 UBE2I 13.17 11.22
1049 SNAP23 13.78 12.23
1050 MTERFD2 10.53 8.59
1051 COTL1 13.89 10.14
1052 PHACTR2 8.65 6.86
1053 GLRA2 5.93 7.69
1054 NUTF2 10.39 9.26
1055 TSGA14 9.52 8.03
1056 FKBP1A 12.16 10.89 12.16 10.82
1057 LRRC1 8.82 7.68
1058 PDIA6 13.07 14.91
1059 YWHAQ 14.50 12.99
1060 ACHE 5.59 8.29
1061 KRAS 12.36 10.49
1062 FAM107B 13.87 12.55
1063 CCDC117 11.03 9.56
1064 WDR1 13.38 11.36
1065 HMGB1 16.36 14.98 16.36 14.97
1066 CCND3 12.69 9.79
1067 SUV39H1 9.28 8.07
1068 RANBP5 11.94 10.87
1069 SLC25A33 10.24 9.00
1070 EDNRA 5.94 7.12
1071 RAB11A 12.94 11.73
1072 DDEF1 11.98 10.54
1073 CXorf15 11.84 10.47
1074 C4orf34 13.65 10.16
1075 SLC44A1 11.10 13.50
1076 EIF2AK3 12.29 13.83
1077 RAP1B 14.21 13.21
1078 CDK2 10.20 8.73 10.20 9.01
1079 JDP2 7.94 5.97
1080 GSTCD 8.86 7.85
1081 TNKS2 10.95 9.62 10.95 9.62
1082 FUZ 7.15 8.96 7.15 8.19
1083 SYPL1 13.56 10.87
1084 EIF4H 13.07 12.02
1085 PIP5K2A 9.65 8.06
1086 RGS2 11.61 14.48
1087 ARF5 10.35 9.13
1088 JAKMIP2 8.57 6.96
1089 RABGAP1 12.19 9.65 12.19 10.85
1090 LRIG1 9.76 10.77 10.77 9.16
1091 CLTA 13.08 11.71
1092 APBB2 7.87 6.39
1093 SMC1A 12.88 11.33
1094 CDC27 9.86 10.88
1095 STT3A 8.64 9.66
1096 ZFAND6 12.01 13.04 13.04 11.95
1097 BZRAP1 8.43 7.15
1098 DPP3 9.41 10.44 10.44 8.39
1099 CNIH 11.94 12.98
1100 TMEM16F 9.42 10.46
1101 ARHGEF7 11.99 13.03 13.03 11.18 13.03 11.60
1102 CAMTA1 10.60 11.64
1103 PFN1 14.03 15.08
1104 USP12 10.18 11.23
1105 AZIN1 11.24 12.29
1106 GNA13 13.89 14.94 14.94 13.37
1107 RCOR1 10.46 11.51 11.51 9.96
1108 USP1 13.36 11.52
1109 VANGL1 7.47 9.15
1110 CAPZB 11.85 12.91
1111 CDV3 13.86 14.91
1112 ABI1 11.61 12.67 12.67 10.96
1113 ST5 7.21 8.28
1114 TTL 8.63 9.69 9.69 8.03
1115 ANP32E 12.76 13.82 13.82 12.19
1116 USP7 12.27 13.34
1117 BCL11B 7.20 8.27 8.27 9.68
1118 OAZ2 8.98 10.06
1119 PPP2CA 12.04 13.12 13.12 11.89
1120 TSC1 10.58 11.66
1121 CTDSPL2 9.81 10.90 10.90 9.51 10.90 9.68
1122 NEDD4L 7.81 8.90
1123 NAT13 12.06 13.16
1124 ASF1A 9.54 10.64
1125 DPF1 7.18 8.50 7.18 5.84
1126 RAN 13.46 14.57 14.57 12.39
1127 ELAVL1 11.66 12.77 12.77 11.43
1128 GRHL1 5.76 7.24
1129 SMAD2 10.45 11.57
1130 IQWD1 9.70 10.83 10.83 9.73
1131 ETS2 7.50 5.66
1132 CDC25B 10.04 11.18
1133 USP32 10.65 11.78 11.78 10.75
1134 EED 11.76 12.90 12.90 11.50
1135 CTNND2 5.93 7.81
1136 SHROOM3 7.86 9.01 9.01 6.05
1137 PSCD3 6.43 7.57
1138 ATF7 7.83 8.97
1139 CDK6 10.31 11.46
1140 GCH1 12.59 10.17
1141 AP3S1 11.95 13.12
1142 ARL6IP1 12.03 13.20 13.20 11.51
1143 TBC1D1 11.00 12.17 12.17 10.01
1144 ATP2A2 10.75 11.92 11.92 10.76
1145 SORL1 12.49 13.67 13.67 10.58
1146 SOX5 9.30 10.47 10.47 7.33 10.47 9.08
1147 KIAA1411 8.65 9.83
1148 KIAA0922 12.43 13.61 13.61 10.86 13.61 12.35
1149 SSX2IP 7.82 9.00 9.00 7.49
1150 SNRPD1 11.85 13.04
1151 EDD1 11.23 12.42
1152 BLMH 9.01 10.21
1153 PTPLB 11.32 12.52
1154 SLC25A27 7.44 3.28
1155 PGAM1 12.40 13.61 13.61 12.55
1156 HMGA1 11.26 12.47 12.47 10.36
1157 EDEM1 11.34 12.55
1158 PRPF38A 10.27 11.48 11.48 10.00
1159 DCUN1D1 9.82 11.03
1160 ROBO1 7.43 8.64
1161 LRRC59 11.26 12.65
1162 ZNF207 12.29 13.51
1163 GTDC1 9.19 10.42 10.42 9.35
1164 C1orf121 10.47 11.70
1165 NF1 9.23 10.46
1166 CUL3 12.98 14.21 14.21 12.80 14.21 12.99
1167 B4GALT5 8.74 9.97 9.97 8.08 9.97 8.71
1168 GADD45A 8.14 9.37 9.37 11.74
1169 RASD2 5.53 7.35
1170 CD4 6.76 8.01 8.01 6.11
1171 WWC1 6.16 7.88
1172 DUSP2 9.43 10.69 10.69 9.18
1173 TP53INP2 8.44 9.70 9.70 8.53 9.70 8.00
1174 NRAS 8.89 10.16
1175 TFRC 12.12 13.39 13.39 11.88
1176 MASTL 8.76 10.03 10.03 8.23 10.03 8.64
1177 USP6 9.12 10.39 10.39 9.25
1178 VGLL4 10.30 11.57 11.57 10.16
1179 C10orf78 8.93 10.21
1180 BTG3 9.87 11.16
1181 TMOD2 8.64 9.93 9.93 6.94
1182 HOXA5 7.03 8.32
1183 AK2 10.33 11.62
1184 MAP2K1 10.41 11.71 11.71 10.69 11.71 10.61
1185 CASP3 10.16 11.47
1186 STK40 9.30 10.60 10.60 8.13
1187 GRHL3 5.20 7.46
1188 SLC25A4 8.65 9.96
1189 KLHDC5 9.52 10.83
1190 SAMHD1 9.72 11.03
1191 PHLPP 7.93 9.24 9.24 7.58
1192 CCNE1 7.86 9.18
1193 EGR1 11.92 13.24
1194 PIM1 9.44 10.76 10.76 8.87
1195 HECW1 6.49 7.81
1196 CHAC1 6.73 8.76
1197 P4HA2 6.84 8.18
1198 PPM1E 6.59 7.92
1199 KIAA0746 13.65 14.98
1200 LOC401720 6.33 7.63
1201 CAPN5 5.90 7.24
1202 DYNLL1 12.69 14.03
1203 EHMT1 10.40 11.75
1204 RIC8B 7.54 8.89
1205 GRIN1 7.40 8.93
1206 DEK 12.73 14.09 14.09 10.29
1207 E2F1 7.01 8.37 8.37 7.21
1208 FAM45A 10.66 12.02
1209 VAMP1 11.10 12.47
1210 LTBP1 7.23 8.61
1211 SOCS1 9.18 10.56 10.56 9.25
1212 ZCCHC14 6.98 8.36
1213 KLHL3 8.04 9.43
1214 RET 5.90 7.29 7.29 5.99
1215 CUTL1 10.33 11.72 11.72 10.67 11.72 10.50
1216 RBL1 7.58 8.98 8.98 7.52 8.98 7.97
1217 TOP1 11.47 12.87
1218 GPD1L 7.94 9.35
1219 SAR1B 9.03 10.46 10.46 12.55
1220 MTF2 11.84 13.27 13.27 11.18
1221 ANP32B 15.53 14.21
1222 CIT 8.19 9.63 9.63 7.43 9.63 7.77
1223 POU3F1 6.08 7.52
1224 MTMR12 11.44 12.89 12.89 11.13 12.89 11.60
1225 MBOAT2 6.73 8.17 8.17 6.25
1226 DOCK9 9.28 10.73
1227 ZAK 8.60 10.05 10.05 8.21
1228 LOC152485 11.05 12.51 12.51 10.56
1229 HNRPA3 14.33 12.26
1230 LMNB1 10.63 8.78
1231 ZFYVE21 8.42 9.90
1232 TXNDC5 12.47 16.40
1233 UBE2G1 11.36 12.84
1234 KIF23 7.44 8.93 8.93 5.57 8.93 6.64
1235 DPYSL2 9.98 11.46 11.46 9.03
1236 ATP5G3 12.15 13.64
1237 GLRX5 10.08 11.57
1238 NLK 10.41 11.92
1239 ARL6IP6 10.51 12.02 12.02 10.43
1240 CNNM4 6.17 7.68 7.68 5.21
1241 TBC1D4 9.12 10.64 10.64 8.94
1242 CD163 5.49 7.00
1243 PKD2 8.17 9.71 9.71 7.48
1244 DIAPH3 8.23 9.77 9.77 8.34
1245 RAD23B 10.65 12.20 12.20 10.54
1246 DCAMKL2 5.53 7.08 7.08 5.88
1247 LMBR1 7.22 8.77
1248 RRAS2 11.24 12.79 12.79 11.03
1249 MYO1D 6.31 7.88 7.88 10.07
1250 KLHL5 10.57 12.15 12.15 10.43 12.15 10.80
1251 EPS15 12.32 13.91 13.91 12.70
1252 FASLG 5.47 7.06
1253 H2AFY 12.56 14.16
1254 LIMA1 8.47 10.07 10.07 9.06
1255 CDCA4 8.92 10.52 10.52 9.16
1256 HAS3 5.70 7.31 7.31 6.15
1257 HRBL 5.96 7.57 7.57 6.35 7.57 5.97
1258 SYAP1 9.71 11.32 11.32 9.76
1259 MDFIC 12.06 13.67
1260 FAM76B 11.27 12.89 12.89 10.52
1261 SNTB2 8.22 9.85 9.85 8.64
1262 ARL3 8.92 10.55
1263 GPR124 5.50 7.14
1264 BCL2L11 10.79 12.43 12.43 11.14
1265 RNF103 12.03 13.23
1266 MYB 6.97 8.62 8.62 5.61
1267 PKM2 11.21 12.87
1268 VCL 8.92 10.57 10.57 8.25
1269 RBBP7 12.45 14.11 14.11 12.96 14.11 12.70
1270 LBR 12.86 14.53 14.53 12.16 14.53 12.50
1271 RRBP1 6.97 8.64 8.64 11.94
1272 GABRB3 5.33 7.00
1273 SGCB 7.78 9.45 9.45 8.22
1274 FAM81A 6.61 8.30 8.30 5.44
1275 RAB15 5.75 7.44
1276 SOX9 6.72 8.42
1277 SAP30 8.22 9.91
1278 BRWD1 8.60 10.30 10.30 8.72 10.30 9.04
1279 KCNMA1 7.47 9.17 9.17 10.42
1280 WHSC1 9.67 11.37 11.37 9.60
1281 CCDC126 9.36 11.09
1282 GRAMD1C 7.83 9.57
1283 PHF19 8.98 10.73 10.73 9.18
1284 ADAM23 7.02 8.77 8.77 7.00
1285 C9orf150 5.38 7.13
1286 ZNF572 6.52 8.30 8.30 6.82
1287 STK39 9.38 11.15 11.15 9.40
1288 SMS 9.73 11.54
1289 DMD 8.96 10.77 10.77 9.32
1290 C1orf83 6.15 7.96
1291 MFHAS1 9.80 11.61 11.61 9.36
1292 STXBP1 6.57 7.79
1293 CPNE2 5.44 7.27 7.27 4.84
1294 MYH10 7.07 8.90 8.90 7.85
1295 CALM3 9.63 11.46 11.46 10.30
1296 EFNB2 7.01 8.85
1297 ACTN1 5.89 7.74
1298 RBMS3 5.67 7.51
1299 ACOT7 9.70 7.83
1300 RKHD1 5.92 7.77
1301 LRRK1 9.74 11.62 11.62 10.12
1302 PTCH1 7.70 9.58
1303 MGLL 7.30 9.18 9.18 10.57 9.18 8.06
1304 YWHAH 8.94 10.82 10.82 9.09
1305 PDE4D 6.78 8.66
1306 MAF 8.05 9.95 9.95 8.25
1307 PTGER3 5.18 7.09 7.09 6.06
1308 PRKCD 9.49 11.40
1309 CCDC64 8.15 10.07 10.07 7.55
1310 RASL11A 7.89 6.65
1311 KPNA2 11.18 13.09 13.09 11.78 13.09 11.55
1312 GPR137B 8.65 10.58 10.58 9.36
1313 TIAM1 6.97 8.90
1314 TFDP1 10.95 12.90 12.90 10.49
1315 SSBP2 10.47 12.42 12.42 10.32
1316 REEP1 5.64 7.61
1317 MAP2 7.85 9.83 9.83 7.75
1318 HOXA9 5.15 7.12
1319 SCRN1 11.81 9.54
1320 LOC129607 8.79 10.78 10.78 9.33 10.78 9.45
1321 SIAH2 10.35 12.34 12.34 10.57
1322 DKFZP564O0823 6.96 8.95
1323 POLQ 8.30 10.30 10.30 8.98 10.30 7.83
1324 KLF15 5.88 7.88 7.88 9.36 7.88 6.74
1325 PXDN 8.36 10.37
1326 BTBD12 8.06 10.08 10.08 8.56
1327 PHF6 11.19 13.21 13.21 11.96 13.21 12.09
1328 SLC41A2 5.67 7.68
1329 HN1 9.87 11.92 11.92 9.85 11.92 10.58
1330 ZNF608 8.98 11.07 11.07 9.74
1331 RNGTT 10.32 12.45
1332 RAP2A 10.35 12.47
1333 LIMK1 5.18 7.36 7.36 5.05
1334 SMAD1 5.91 8.10 8.10 6.34
1335 NCOA7 10.39 12.58
1336 PRDM15 10.29 12.49
1337 PELI1 10.67 12.88
1338 PLS1 5.43 7.65 7.65 4.53
1339 RAB23 6.14 8.36
1340 NAP1L5 7.40 9.63 9.63 8.30 9.63 8.34
1341 DNER 6.90 9.14
1342 LRRC42 7.58 9.83
1343 ID2 7.74 9.99
1344 IBRDC2 9.98 12.24 12.24 8.84 12.24 9.94
1345 DNMT1 11.21 13.50
1346 STAC3 5.74 8.02 8.02 7.01
1347 HMGB3 8.20 10.50 10.50 8.80 10.50 8.08
1348 BMPR1A 6.75 9.06
1349 SGK 10.06 12.37
1350 CBX2 6.06 8.37 8.37 6.38
1351 LRRC20 5.69 8.03
1352 LRRC4 4.81 7.15 7.15 5.05
1353 HOXA1 5.36 7.70
1354 LRRC62 4.95 7.30
1355 ATAD2 9.00 11.34 11.34 8.44 11.34 9.37
1356 MOBKL1A 9.73 12.08 12.08 9.13 12.08 10.12
1357 LOC220594 10.06 12.41 12.41 10.07
1358 ZNF804A 6.14 8.51
1359 C1orf113 5.99 8.36 8.36 5.74
1360 FMNL2 6.58 8.96
1361 H2AFX 12.49 10.60
1362 ATP1B1 5.60 8.03
1363 GPT2 6.54 8.97
1364 PSRC1 9.89 7.46
1365 SLC25A35 5.23 7.68
1366 LHFPL2 8.94 11.41
1367 UBE2J1 11.34 13.81 13.81 12.22
1368 TBC1D8B 5.43 7.91 7.91 6.89
1369 SGPP1 10.96 13.46 13.46 11.28
1370 C11orf9 4.74 7.24
1371 BCL6 12.29 14.82 14.82 10.60 14.82 11.78
1372 ANUBL1 8.12 10.66 10.66 8.78
1373 MTA3 8.37 10.92
1374 PGBD5 4.87 7.45 7.45 5.87
1375 LPP 11.00 13.59 13.59 10.07 13.59 10.89
1376 NDFIP2 7.39 9.99
1377 STMN1 11.76 9.03
1378 PITPNC1 8.77 11.42 11.42 10.00
1379 SH3RF1 7.37 10.05 10.05 8.10
1380 ASF1B 8.56 11.25 11.25 7.91 11.25 8.27
1381 FLJ20186 12.06 14.82 14.82 12.05
1382 SLC16A2 8.08 5.17
1383 PEX5 9.12 11.90 11.90 9.50
1384 ECT2 7.45 10.23 10.23 7.70 10.23 7.63
1385 MAML3 8.05 10.85
1386 TEAD1 4.42 7.24
1387 HMGB2 12.96 15.78 15.78 12.73 15.78 13.36
1388 NCALD 5.15 7.97
1389 RGC32 7.75 10.62
1390 PPP1R3C 4.18 7.06
1391 DEPDC1B 9.96 7.11
1392 WEE1 10.11 13.01 13.01 10.32 13.01 11.84
1393 FHL2 4.82 7.77
1394 ITGB8 4.70 7.66
1395 SLC1A1 10.86 8.88
1396 FAM83D 7.36 10.32
1397 UHRF1 12.22 8.72
1398 C7orf41 4.94 7.95 7.95 6.29
1399 ZBTB8 5.58 8.59 8.59 11.07
1400 ZNF367 7.97 10.99 10.99 8.04 10.99 8.22
1401 CDC25A 6.03 9.09 9.09 6.81
1402 CHEK1 7.59 10.68 10.68 7.72
1403 CDCA7 10.61 13.76 13.76 10.16 13.76 11.06
1404 FGF13 4.55 7.69
1405 SSBP3 6.20 9.35
1406 EZH2 9.40 12.57 12.57 10.22
1407 TNFSF11 5.18 8.36 8.36 5.91 8.36 9.78
1408 PACSIN1 5.16 8.35
1409 RRM2 10.59 13.82 13.82 9.47 13.82 10.79
1410 POU4F1 5.62 8.85
1411 MYBL2 9.07 12.41 12.41 9.12
1412 KIAA1212 8.46 11.81 11.81 8.55
1413 CCNE2 7.30 10.66 10.66 8.02 10.66 8.03
1414 IGF2BP3 7.92 11.34 11.34 9.93 11.34 9.55
1415 PRKAR2B 6.17 9.66 9.66 8.16
1416 CADPS 5.04 8.58 8.58 6.18
1417 ANKRD15 6.97 10.52 10.52 7.58
1418 DAAM1 9.33 12.92
1419 MAP1B 4.05 7.68 7.68 5.03 7.68 6.23
1420 AFF2 6.32 10.00
1421 E2F7 5.66 9.37 9.37 6.53 9.37 6.89
1422 PRC1 8.37 12.08
1423 ENPP5 3.54 7.26 7.26 4.97
1424 E2F8 7.68 11.41
1425 MED12L 7.82 11.59 11.59 7.46 11.59 10.57
1426 LOC162073 6.30 10.08
1427 LRRC32 4.14 7.93 7.93 6.61 7.93 4.95
1428 DMXL2 6.57 10.38 10.38 6.61 10.38 7.76
1429 FLJ20366 7.86 11.68 11.68 7.62 11.68 9.86
1430 TOX 9.05 12.88 12.88 11.27
1431 MME 11.56 10.33
1432 FGD6 7.63 11.73 11.73 9.12
1433 MYBL1 9.68 14.13 14.13 10.02 14.13 9.72
*Empty cells indicate values not appreciably measured.
TABLE 16
Predictor microRNAs that distinguish activated B-cell (ABC)
DLBCL from germinal center B-cell (GCB) DLBC
Higher
ABC vs GCB in
hsa-miR-93/mmu-miR-93/rno-miR-93 GCB
hsa-miR-331-3p/mmu-miR-331-3p/rno-miR-331 GCB
hsa-miR-129* GCB
hsa-miR-423-3p/mmu-miR-423-3p/rno-miR-423 GCB
hsa-miR-28-5p/mmu-miR-28/rno-miR-28 GCB
mghv-miR-M1-7-3p GCB
hsa-miR-518b GCB
ebv-miR-BHRF1-1 GCB
hsa-miR-140-5p/mmu-miR-140/rno-miR-140 GCB
hsa-miR-505* GCB
hsa-miR-675 GCB
hsa-miR-198 GCB
hsa-miR-125b-1*/mmu-miR-125b-3p/mo-miR- GCB
125b-3p
hsa-miR-361-5p/mmu-miR-361/rno-miR-361 GCB
ebv-miR-BART8* GCB
TABLE 17
Predictor microRNAs that distinguish activated B-cell (ABC)
DLBCL from Burkitt lymphoma
Higher
ABC vs BL in
hsa-miR-155 ABC
hsa-miR-29c/mmu-miR- ABC
29c/rno-miR-29c
hsa-miR-146b-5p/mmu-miR- ABC
146b/rno-miR-146b
hsa-miR-29b/mmu-miR- ABC
29b/rno-miR-29b
hsa-miR-22/mmu-miR- ABC
22/rno-miR-22
hsa-miR-21/mmu-miR- ABC
21/rno-miR-21
hsa-miR-768-3p ABC
hsa-miR-145/mmu-miR- ABC
145/rno-miR-145
hsa-miR-29a/mmu-miR- ABC
29a/rno-miR-29a
hsa-miR-30e/mmu-miR- ABC
30e/rno-miR-30e
hsa-miR-26a/mmu-miR- ABC
26a/rno-miR-26a
hsa-miR-101/mmu-miR- ABC
101a/rno-miR-101a
hsa-miR-24/mmu-miR- ABC
24/rno-miR-24
hsa-miR-26b/mmu-miR- ABC
26b/rno-miR-26b
hsa-miR-27a/mmu-miR- ABC
27a/rno-miR-27a
hsa-miR-27b/mmu-miR- ABC
27b/rno-miR-27b
hsa-miR-23b/mmu-miR- ABC
23b/rno-miR-23b
hsa-miR-23a/mmu-miR- ABC
23a/rno-miR-23a
hsa-miR-125b/mmu-miR- ABC
125b-5p/rno-miR-125b-5p
hsa-miR-30a/mmu-miR- ABC
30a/rno-miR-30a
hsa-miR-142-3p/mmu-miR- ABC
142-3p/rno-miR-142-3p
hsa-let-7a/mmu-let-7a/rno-let- ABC
7a
hsa-miR-30b/mmu-miR- ABC
30b/rno-miR-30b-5p
hsa-miR-142-5p/mmu-miR- ABC
142-5p/rno-miR-142-5p
hsa-miR-34b/mmu-miR-34b-3p ABC
hsa-miR-16/mmu-miR-16/rno- ABC
miR-16
hsa-miR-30c/mmu-miR- ABC
30c/rno-miR-30c
hsa-let-7c/mmu-let-7c/rno-let- ABC
7c
hsa-miR-550 ABC
hsa-miR-921 BL
hsa-miR-30c-2*/mmu-miR- BL
30c-2*/rno-miR-30c-2*
hsa-miR-933 BL
hsa-miR-658 BL
hsa-miR-628-3p BL
hsa-miR-503 BL
hsa-miR-193a-5p BL
hsa-miR-30b* BL
hsa-miR-93/mmu-miR-93/rno- BL
miR-93
hsa-miR-18b BL
hsa-miR-18a/mmu-miR- BL
18a/rno-miR-18a
hsa-miR-874/mmu-miR- BL
874/rno-miR-874
TABLE 18
Predictor microRNAs that distinguish activated B-cell (ABC)
DLBCL from chronic lymphocytic leukemia
Higher
ABC vs CLL in
hsa-miR-125b/mmu-miR- ABC
125b-5p/rno-miR-125b-5p
hsa-miR-126/mmu-miR- ABC
126-3p/rno-miR-126
hsa-miR-199a-3p/hsa- ABC
miR-199b-3p/mmu-miR-
199a-3p/mmu-miR-
199b/rno-miR-199a-3p
hsa-miR-145/mmu-miR- ABC
145/rno-miR-145
hsa-miR-143/mmu-miR- ABC
143/rno-miR-143
hsa-miR-637 ABC
hsa-miR-371-5p ABC
kshv-miR-K12-6-3p ABC
hsa-miR-628-3p ABC
hsa-miR-126*/mmu-miR- ABC
126-5p/rno-miR-126*
hsa-miR-193a-5p ABC
hsa-miR-21/mmu-miR- ABC
21/rno-miR-21
hsa-miR-24/mmu-miR- ABC
24/rno-miR-24
hsa-miR-503 ABC
hsa-miR-23a/mmu-miR- ABC
23a/rno-miR-23a
hsa-miR-23b/mmu-miR- ABC
23b/rno-miR-23b
hsa-miR-22/mmu-miR- ABC
22/rno-miR-22
hsa-miR-665 ABC
hsa-let-7c/mmu-let-7c/rno- ABC
let-7c
hsa-miR-658 ABC
hsa-let-7a/mmu-let-7a/rno- ABC
let-7a
mghv-miR-M1-4 ABC
hsa-miR-933 ABC
hsa-miR-550 CLL
hsa-miR-30e/mmu-miR- CLL
30e/rno-miR-30e
hsa-miR-142- CLL
5p/mmu-miR-142-
5p/rno-miR-142-5p
hsa-miR-101/mmu- CLL
miR-101a/rno-miR-
101a
hsa-miR-185/mmu- CLL
miR-185/rno-miR-185
hsa-miR-888* CLL
hsa-miR-199a- CLL
5p/mmu-miR-199a-
5p/rno-miR-199a-5p
hsa-miR-668/mmu- CLL
miR-668
hsa-miR-549 CLL
hsa-miR-801/mmu- CLL
miR-801
hsa-miR-649 CLL
hsa-miR-625* CLL
hsa-miR-140- CLL
3p/mmu-miR-
140*/rno-miR-140*
hsa-let-7f/mmu-let- CLL
7f/rno-let-7f
hsa-miR-768-5p CLL
hsa-miR-24-1*/mmu- CLL
miR-24-1*/rno-miR-
24-1*
ebv-miR-BART13 CLL
hsa-miR-339- CLL
5p/mmu-miR-339-
5p/rno-miR-339-5p
hsa-miR-20b/mmu- CLL
miR-20b/rno-miR-
20b-5p
hsa-miR-335/mmu- CLL
miR-335-5p/rno-miR-
335
mghv-miR-M1-8 CLL
hsa-miR-30d/mmu- CLL
miR-30d/rno-miR-30d
hsa-miR-363/mmu- CLL
miR-363/rno-miR-363
hsa-miR-361-3p CLL
hsa-miR-107/mmu- CLL
miR-107/rno-miR-107
hsa-miR-154/mmu- CLL
miR-154/rno-miR-154
hsa-miR-638 CLL
hsa-miR-340/mmu- CLL
miR-340-5p/rno-miR-
340-5p
hsa-miR-331- CLL
3p/mmu-miR-331-
3p/rno-miR-331
hsa-miR-151- CLL
5p/mmu-miR-151-
5p/rno-miR-151
hsa-miR-636 CLL
hsa-miR-33a/mmu- CLL
miR-33/rno-miR-33
hsa-miR-486- CLL
5p/mmu-miR-486
hsa-miR-150/mmu- CLL
miR-150/rno-miR-
150
hsa-miR-25/mmu- CLL
miR-25/rno-miR-25
hsa-miR-331- CLL
5p/mmu-miR-331-5p
hsa-miR-299- CLL
5p/mmu-miR-
299*/rno-miR-299
hsa-miR-891a CLL
hsa-miR-144* CLL
hsa-miR-363*/rno- CLL
miR-363*
hsa-miR-93/mmu- CLL
miR-93/rno-miR-93
hsa-miR-423- CLL
3p/mmu-miR-423-
3p/rno-miR-423
hsa-let-7g/mmu-let- CLL
7g
hsa-miR-28-5p/mmu- CLL
miR-28/rno-miR-28
hsa-miR-20b* CLL
hsa-miR-140- CLL
5p/mmu-miR-
140/rno-miR-140
hsa-miR-519d CLL
hsa-miR-147 CLL
hsa-miR-487b/mmu- CLL
miR-487b/rno-miR-
487b
hsa-miR-361- CLL
5p/mmu-miR-
361/rno-miR-361
hsa-miR-186/mmu- CLL
miR-186/rno-miR-
186
hsa-miR-32/mmu- CLL
miR-32/rno-miR-32
hsa-miR-129* CLL
hsa-miR-30e*/mmu- CLL
miR-30e*/rno-miR-
30e*
hsa-miR-196a*/mmu- CLL
miR-196a*/rno-miR-
196a*
TABLE 19
Predictor microRNAs that distinguish activated B-cell (ABC)
DLBCL from follicular lymphoma
Higher
ABC vs FL in
hsa-miR-938 ABC
hsa-miR-183*/mmu-miR- ABC
183*
hsa-miR-197/mmu-miR- ABC
197
hsa-miR-382/mmu-miR- ABC
382/rno-miR-382
hsa-miR-20b* ABC
hsa-miR-524-5p ABC
hsa-miR-337-3p ABC
hsa-miR-600 ABC
hsa-miR-96/mmu-miR- ABC
96/rno-miR-96
mghv-miR-M1-8 ABC
hsa-miR-29c*/mmu-miR- ABC
29c*/rno-miR-29c*
hsa-miR-575 ABC
hsa-miR-518a-3p ABC
hsa-miR-361-5p/mmu-miR- ABC
361/rno-miR-361
hsa-miR-193b* ABC
hsa-miR-340*/mmu-miR- ABC
340-3p/rno-miR-340-3p
hsa-miR-708/mmu-miR- ABC
708/rno-miR-708
hsa-miR-129* ABC
hsa-miR-525-5p ABC
hsa-miR-497/mmu-miR- FL
497/rno-miR-497
hsa-miR-22*/mmu-miR- FL
22*/rno-miR-22*
hsa-miR-130b/mmu-miR- FL
130b/rno-miR-130b
hsa-miR-551b* FL
hsa-miR-331-3p/mmu-miR- FL
331-3p/rno-miR-331
ebv-miR-BART13 FL
hsa-miR-877/mmu-miR- FL
877/rno-miR-877
hsa-miR-636 FL
hsa-miR-922 FL
hsa-miR-198 FL
hsa-miR-342-5p/mmu-miR- FL
342-5p/rno-miR-342-5p
hsa-miR-585 FL
ebv-miR-BART8* FL
hsa-miR-617 FL
hsa-miR-221* FL
hsa-miR-125b-1*/mmu- FL
miR-125b-3p/rno-miR-
125b-3p
hsa-miR-93/mmu-miR- FL
93/rno-miR-93
hsa-miR-363*/rno-miR- FL
363*
hsa-miR-744/mmu-miR- FL
744
hsa-miR-659 FL
hsa-miR-490-3p/mmu-miR- FL
490
hsa-let-7d/mmu-let-7d/rno- FL
let-7d
hsa-miR-361-3p FL
ebv-miR-BHRF1-1 FL
hsa-miR-92b/mmu-miR- FL
92b/rno-miR-92b
hsa-miR-151-5p/mmu-miR- FL
151-5p/rno-miR-151
hsa-miR-144* FL
hsa-miR-425/mmu-miR- FL
425/rno-miR-425
hsa-miR-138/mmu-miR- FL
138/rno-miR-138
hsa-miR-92a/mmu-miR- FL
92a/rno-miR-92a
hsa-miR-151-3p FL
hsa-miR-25/mmu-miR- FL
25/rno-miR-25
hsa-miR-509-3-5p FL
hsa-miR-30e*/mmu-miR- FL
30e*/rno-rniR-30e*
hsa-miR-28-5p/mmu-miR- FL
28/rno-miR-28
hsa-miR-200b*/mmu-miR- FL
200b*
hsa-miR-148b/mmu-miR- FL
148b/rno-miR-148b-3p
hsa-miR-488 FL
hsa-miR-99b/mmu-miR- FL
99b/rno-miR-99b
hsa-miR-339-5p/mmu-miR- FL
339-5p/rno-miR-339-5p
hsv1-miR-H1 FL
hsa-miR-32/mmu-miR- FL
32/rno-miR-32
hsa-miR-885-5p FL
hsa-miR-630 FL
ebv-miR-BART16 FL
hsa-miR-505* FL
hsa-miR-374b* FL
hsa-miR-574-3p/mmu-miR- FL
574-3p
hsa-miR-874/mmu-miR- FL
874/rno-miR-874
hsa-miR-423-3p/mmu-miR- FL
423-3p/rno-miR-423
hsa-miR-889 FL
hcmv-miR-UL148D FL
hsa-miR-487b/mmu-miR- FL
487b/rno-miR-487b
hsa-miR-552 FL
hsa-miR-220b FL
hsa-miR-551a FL
hsa-let-7d*/mmu-let- FL
7d*/rno-let-7d*
kshv-miR-K12-5 FL
hsa-miR-629* FL
hsa-miR-99b*/mmu-miR- FL
99b*/rno-miR-99b*
hsa-miR-615-3p/mmu-miR- FL
615-3p
hsa-miR-657 FL
hsa-miR-301a/mmu-miR- FL
301a/rno-miR-301a
hsa-miR-518b FL
hsa-miR-194/mmu- FL
miR-194/rno-miR-
194
hsa-miR-647 FL
kshv-miR-K12-6-5p FL
hsa-miR-622 FL
hsa-miR-516b FL
hsa-miR-675 FL
hsa-miR-526b FL
hsa-miR-671- FL
5p/mmu-miR-671-5p
hsa-miR-18a/mmu- FL
miR-18a/rno-miR-
18a
hsa-miR-18b FL
hsa-miR-181b/mmu- FL
miR-181b/rno-miR-
181b
hsa-miR-215 FL
hsa-miR-153/mmu- FL
miR-153/rno-miR-
153
hsa-miR-625 FL
hsa-miR-510 FL
hsa-miR-519d FL
mghv-miR-M1-7-3p FL
hsa-miR-485- FL
3p/mmu-miR-485*
hsa-miR-483-5p FL
hsa-miR-140- FL
5p/mmu-miR-
140/rno-miR-140
hsa-miR-921 FL
hsa-miR-186/mmu- FL
miR-186/rno-miR-
186
hsa-miR-196a*/mmu- FL
miR-196a*/rno-miR-
196a*
hsa-miR-381/mmu- FL
miR-381/rno-miR-
381
hsa-miR-620 FL
hsa-miR-152/mmu- FL
miR-152/rno-miR-
152
hsa-miR-766 FL
mghv-miR-M1-7-5p FL
hsa-miR-374b/mmu- FL
miR-374/rno-miR-
374
hsa-let-7c/mmu-let- FL
7c/rno-let-7c
kshv-miR-K12-8 FL
mghv-miR-M1-3 FL
hsa-miR-920 FL
hsa-miR-519e* FL
hsa-miR-147 FL
hsa-miR-424 FL
hsa-miR-193b FL
ebv-miR-BART19-3p FL
hsa-miR-146b-3p FL
hsa-miR-30c/mmu- FL
miR-30c/rno-miR-
30c
hsa-miR-30a/mmu- FL
miR-30a/rno-miR-
30a
hsa-miR-939 FL
hsa-let-7a/mmu-let- FL
7a/rno-let-7a
hsa-miR-122* FL
hsa-miR-206/mmu- FL
miR-206/rno-miR-
206
ebv-miR-BART18-3p FL
hsa-miR-183/mmu- FL
miR-183/rno-miR-
183
hsa-miR-9*/mmu- FL
miR-9*/rno-miR-9*
kshv-miR-K12-1 FL
hsa-miR-34c- FL
5p/mmu-miR-
34c/rno-miR-34c
hsa-miR-934 FL
hsa-miR-890 FL
hsa-miR-514 FL
hsa-miR-297/mmu- FL
miR-297a
hsa-miR-553 FL
hsa-miR-765 FL
hsa-let-7b*/mmu-let- FL
7b*/rno-let-7b*
hsa-miR-500* FL
hsa-miR-601 FL
ebv-miR-BHRF1-3 FL
hsa-miR-296- FL
3p/mmu-miR-296-
3p/rno-miR-296
hsa-miR-574- FL
5p/mmu-miR-574-5p
hsa-miR-409- FL
5p/mmu-miR-409-
5p/rno-miR-409-5p
hsa-miR-195* FL
hsa-miR-635 FL
hsa-miR-542- FL
3p/mmu-miR-542-
3p/rno-miR-542-3p
hcmv-miR-US25-1* FL
mghv-miR-M1-2 FL
hsa-miR-509-5p FL
hsa-miR-340/mmu- FL
miR-340-5p/rno-
miR-340-5p
hsa-miR-891a FL
hsa-miR-23a/mmu- FL
miR-23a/rno-miR-
23a
hsa-miR-19b/mmu- FL
miR-19b/rno-miR-
19b
hsa-miR-515-5p FL
hsa-miR-23b/mmu- FL
miR-23b/rno-miR-
23b
hsa-miR-498 FL
hsa-miR-886-5p FL
hsa-miR-220c FL
hsa-miR-10a/mmu- FL
miR-10a/rno-miR-
10a-5p
hsa-miR-32* FL
hsa-miR-24/mmu- FL
miR-24/rno-miR-24
hsa-miR-98/mmu- FL
miR-98/rno-miR-98
hsa-let-7g/mmu-let-7g FL
hsa-miR-302a/mmu- FL
miR-302a
hsa-miR-625* FL
hsa-miR-30b* FL
hsa-miR-30b/mmu- FL
miR-30b/rno-miR-
30b-5p
hsa-miR-377* FL
hsa-miR-106b*/mmu- FL
miR-106b*/rno-miR-
106b*
hsa-miR-181a-2* FL
hsa-miR-887 FL
hsa-miR-208a/mmu- FL
miR-208a/rno-miR-
208
ebv-miR-BART7* FL
hsa-miR-7-2* FL
hsa-miR-155* FL
hsa-miR-513a-3p FL
kshv-miR-K12-7 FL
hsa-miR-299-3p FL
hsa-miR-218-2*/mmu- FL
miR-218-2*/rno-miR-
218*
hsa-miR-130b*/mmu- FL
miR-130b*
hsa-miR-620 FL
hsa-miR-33a/mmu- FL
miR-33/rno-miR-33
hsa-miR-7/mmu-miR- FL
7a/rno-miR-7a
ebv-miR-BART6-3p FL
hsa-miR-22/mmu- FL
miR-22/rno-miR-22
hsa-miR-199b-5p FL
hsa-miR-768-3p FL
hsa-miR-494/mmu- FL
miR-494/rno-miR-494
hsa-miR-602 FL
hsa-miR-125b-2*/rno- FL
miR-125b*
hsa-miR-300 FL
hsa-let-7e/mmu-let- FL
7e/rno-let-7e
hsa-miR-298 FL
hsa-miR-576-3p FL
hsa-miR-187* FL
hsa-miR-365/mmu- FL
miR-365/rno-miR-365
hsa-miR-518a-5p/hsa- FL
miR-527
hsa-miR-302d* FL
hsa-miR-105 FL
hsa-miR-126/mmu- FL
miR-126-3p/rno-miR-
126
hsa-miR-107/mmu- FL
miR-107/rno-miR-107
hsa-miR-299-5p/mmu- FL
miR-299*/rno-miR-
299
hsa-miR-28-3p/rno- FL
miR-28*
hsa-miR-21/mmu- FL
miR-21/rno-miR-21
hsa-miR-27b/mmu- FL
miR-27b/rno-miR-27b
hsa-miR-516a-5p FL
hsa-miR-129-5p/mmu- FL
miR-129-5p/rno-miR-
129
hsa-miR-583 FL
hsa-miR-483-3p FL
hsa-miR-326/mmu- FL
miR-326/rno-miR-326
hsa-miR-548d-5p FL
hsa-miR-629 FL
ebv-miR-BART5 FL
hsa-miR-665 FL
hsa-miR-493 FL
hsa-miR-484/mmu- FL
miR-484/rno-miR-484
hsa-miR-645 FL
hsa-miR-452 FL
hsa-miR-518c* FL
hsa-miR-24-2*/mmu- FL
miR-24-2*/rno-miR-
24-2*
hsa-miR-124/mmu- FL
miR-124/rno-miR-124
hsa-miR-184/mmu- FL
miR-184/rno-miR-184
hsa-miR-27a*/mmu- FL
miR-27a*/rno-miR-
27a*
hsa-miR-25* FL
hsa-miR-34b/mmu- FL
miR-34b-3p
ebv-miR-BART17-5p FL
hsa-miR-658 FL
hsa-miR-212/mmu- FL
miR-212/rno-miR-212
hsa-miR-99a/mmu- FL
miR-99a/rno-miR-99a
hsa-miR-801/mmu- FL
miR-801
hsa-miR-491-3p FL
hsa-miR-551b/mmu- FL
miR-551b/rno-miR-
551b
hsa-miR-214/mmu- FL
miR-214/rno-miR-214
hsa-miR-30e/mmu- FL
miR-30e/rno-miR-30e
hsa-miR-888* FL
hsa-miR-505/rno-miR- FL
505
hsa-miR-27a/mmu- FL
miR-27a/rno-miR-27a
hsa-miR-15a/mmu- FL
miR-15a
hsa-miR-20a/mmu- FL
miR-20a/rno-miR-20a
kshv-miR-K12-3 FL
hsa-miR-455-3p FL
hsa-miR-486-5p/mmu- FL
miR-486
TABLE 20
Predictor microRNAs that distinguish activated B-cell (ABC)
DLBCL from Hodgkin's lymphoma
Higher
ABC vs HL in
hsa-miR-19b/mmu-miR- ABC
19b/rno-miR-19b
hsa-miR-30b/mmu-miR- ABC
30b/rno-miR-30b-5p
hsa-miR-142-3p/mmu- ABC
miR-142-3p/rno-miR-142-
3p
hsa-miR-768-3p ABC
hsa-miR-30c/mmu-miR- ABC
30c/rno-miR-30c
hsa-miR-29b/mmu-miR- ABC
29b/rno-miR-29b
hsa-miR-24/mmu-miR- ABC
24/rno-miR-24
hsa-miR-22/mmu-miR- ABC
22/rno-miR-22
hsa-miR-30e/mmu-miR- ABC
30e/rno-miR-30e
hsa-miR-30a/mmu-miR- ABC
30a/rno-miR-30a
hsa-miR-26b/mmu-miR- ABC
26b/rno-miR-26b
hsa-miR-26a/mmu-miR- ABC
26a/rno-miR-26a
hsa-miR-15a/mmu-miR- ABC
15a
hsa-miR-16/mmu-miR- ABC
16/rno-miR-16
hsa-miR-142-5p/mmu- ABC
miR-142-5p/rno-miR-142-
5p
hsa-miR-29a/mmu-miR- ABC
29a/rno-miR-29a
hsa-miR-101/mmu-miR- ABC
101a/rno-miR-101a
hsa-miR-126/mmu-miR- ABC
126-3p/rno-miR-126
hsa-miR-451/mmu-miR- ABC
451/rno-miR-451
hsa-let-7a/mmu-let-7a/rno- ABC
let-7a
hsa-miR-23b/mmu-miR- ABC
23b/rno-miR-23b
hsa-miR-21/mmu-miR- ABC
21/rno-miR-21
hsa-miR-29c/mmu-miR- ABC
29c/rno-miR-29c
hsa-miR-20a/mmu-miR- ABC
20a/rno-miR-20a
hsa-miR-27b/mmu-miR- ABC
27b/rno-miR-27b
hsa-miR-23a/mmu-miR- ABC
23a/rno-miR-23a
hsa-miR-27a/mmu-miR- ABC
27a/rno-miR-27a
hsa-miR-550 ABC
hsa-let-7c/mmu-let-7c/rno- ABC
let-7c
hsa-miR-34b/mmu-miR- ABC
34b-3p
hsa-miR-933 HL
hsa-miR-30c-2*/mmu- HL
miR-30c-2*/rno-miR-30c-
2*
hsa-miR-503 HL
hsa-miR-765 HL
hsa-miR-658 HL
hsa-miR-620 HL
hsa-miR-921 HL
hsa-miR-30b* HL
mghv-miR-M1-4 HL
hsa-miR-939 HL
hsa-miR-494/mmu-miR- HL
494/rno-miR-494
hsa-miR-32* HL
hsa-miR-491-3p HL
hsa-miR-10a/mmu-miR- HL
10a/rno-miR-10a-5p
hsa-miR-33a/mmu-miR- HL
33/rno-miR-33
hsa-miR-99a/mmu-miR- HL
99a/rno-miR-99a
hsa-miR-199b-5p HL
hsa-miR-365/mmu-miR- HL
365/rno-miR-365
hsa-miR-520d-5p HL
hsa-miR-518c* HL
hsa-miR-32/mmu-miR- HL
32/rno-miR-32
hsa-miR-214/mmu-miR- HL
214/rno-miR-214
hsa-miR-98/mmu-miR- HL
98/rno-miR-98
hsa-miR-302d* HL
hsa-miR-30e*/mmu-miR- HL
30e*/rno-miR-30e*
hsa-miR-374b/mmu-miR- HL
374/rno-miR-374
hsa-miR-29a*/mmu-miR- HL
29a*/rno-miR-29a*
hsa-miR-532-5p/mmu- HL
miR-532-5p/rno-miR-532-
5p
hsa-miR-149* HL
hsa-miR-422a HL
ebv-miR-BHRF1-2 HL
hsa-miR-634 HL
hsa-miR-143* HL
hsa-miR-620 HL
hsa-miR-660 HL
hsa-miR-140-5p/mmu- HL
miR-140/rno-miR-140
hsa-miR-28-5p/mmu-miR- HL
28/rno-miR-28
hsa-miR-519c-5p/hsa- HL
miR-519b-5p/hsa-miR-
523*/hsa-miR-518e*/hsa-
miR-522*/hsa-miR-519a*
hsa-miR-505/rno-miR-505 HL
hsa-miR-184/mmu-miR- HL
184/rno-miR-184
hsa-miR-107/mmu-miR- HL
107/rno-miR-107
hsa-miR-298 HL
hsa-miR-455-3p HL
hsa-miR-638 HL
hsa-miR-502-3p HL
hsa-miR-149/mmu-miR- HL
149
hsa-miR-583 HL
hsa-miR-105 HL
hsa-miR-128/mmu-miR- HL
128/rno-miR-128
hsa-miR-656 HL
hsa-miR-497/mmu-miR- HL
497/rno-miR-497
hsa-miR-152/mmu-miR- HL
152/rno-miR-152
hsa-miR-151-5p/mmu- HL
miR-151-5p/rno-miR-151
hsa-miR-148b/mmu-miR- HL
148b/rno-miR-148b-3p
hsa-miR-300 HL
hsa-miR-144* HL
hsa-miR-145*/mmu-miR- HL
145*
hcmv-miR-UL70-3p HL
hsa-miR-28-3p/rno-miR- HL
28*
hsa-miR-27a*/mmu-miR- HL
27a*/rno-miR-27a*
hsa-miR-194/mmu-miR- HL
194/rno-miR-194
hsa-miR-130b*/mmu-miR- HL
130b*
hsa-miR-548d-5p HL
hsa-miR-937 HL
hsa-miR-7/mmu-miR- HL
7a/rno-miR-7a
hsa-miR-518a-5p/hsa- HL
miR-527
hsa-miR-323-3p/mmu- HL
miR-323-3p/rno-miR-323
hsa-miR-215 HL
hsa-miR-513a-3p HL
hsa-miR-595 HL
hsa-miR-515-5p HL
hsa-miR-483-3p HL
hsa-miR-330-5p/mmu- HL
miR-330/rno-miR-330
hsa-miR-18b HL
hsa-miR-509-3p HL
hsa-miR-151-3p HL
hsa-miR-934 HL
hsa-miR-328/mmu-miR- HL
328/rno-miR-328
hsa-miR-187* HL
kshv-miR-K12-3 HL
hsa-miR-373* HL
hsa-miR-96/mmu-miR- HL
96/rno-miR-96
hsa-miR-186/mmu-miR- HL
186/rno-miR-186
hsa-miR-886-5p HL
hsa-miR-424 HL
hsa-miR-147 HL
hsa-miR-340/mmu-miR- HL
340-5p/rno-miR-340-5p
hsa-miR-129-5p/mmu- HL
miR-129-5p/rno-miR-129
hsa-miR-25* HL
hsa-miR-193b HL
hsa-miR-574-5p/mmu- HL
miR-574-5p
hsa-miR-589 HL
hsa-miR-339-5p/mmu- HL
miR-339-5p/rno-miR-
339-5p
hsa-miR-34c-5p/mmu- HL
miR-34c/rno-miR-34c
hsa-miR-891a HL
hsa-miR-18a/mmu-miR- HL
18a/rno-miR-18a
hsa-miR-196a*/mmu- HL
miR-196a*/rno-miR-
196a*
hsa-miR-17*/rno-miR- HL
17-3p
hsa-miR-296-5p/mmu- HL
miR-296-5p/rno-miR-
296*
hsa-miR-25/mmu-miR- HL
25/rno-miR-25
hsa-miR-509-5p HL
hsa-miR-550* HL
hsa-miR-708/mmu-miR- HL
708/rno-miR-708
hsa-miR-146b-3p HL
hsa-miR-625* HL
hsa-miR-210/mmu-miR- HL
210/rno-miR-210
hsa-miR-93/mmu-miR- HL
93/rno-miR-93
hsa-miR-548b-3p HL
hsa-miR-652/mmu-miR- HL
652/rno-miR-652
hsa-miR-153/mmu-miR- HL
153/rno-miR-153
mghv-miR-M1-3 HL
hsa-miR-194* HL
hsa-miR-23a*/rno-miR- HL
23a*
hsa-miR-943 HL
hsa-let-7d/mmu-let- HL
7d/rno-let-7d
hsa-miR-498 HL
hsa-miR-381/mmu-miR- HL
381/rno-miR-381
hsa-miR-586 HL
hsa-miR-137/mmu-miR- HL
137/rno-miR-137
hsa-miR-610 HL
hsa-miR-920 HL
hsa-miR-936 HL
hsa-miR-744/mmu-miR- HL
744
ebv-miR-BART5 HL
hsa-miR-21* HL
hsa-miR-516a-5p HL
hsa-miR-576-5p HL
mghv-miR-M1-6 HL
hsa-miR-425/mmu-miR- HL
425/rno-miR-425
hsa-miR-220c HL
hsa-miR-10a*/mmu-miR- HL
10a*/rno-miR-10a-3p
hsa-miR-452 HL
hsa-miR-345 HL
hsa-miR-29c*/mmu-miR- HL
29c*/rno-miR-29c*
hsa-miR-887 HL
hsa-miR-7-2* HL
hsa-miR-363*/rno-miR- HL
363*
hsa-miR-22*/mmu-miR- HL
22*/rno-miR-22*
hsa-miR-922 HL
hsa-miR-92b* HL
hsa-miR-526a/hsa-miR- HL
520c-5p/hsa-miR-518d-
5p
hsa-miR-574-3p/mmu- HL
miR-574-3p
hsa-miR-92a/mmu-miR- HL
92a/rno-miR-92a
hsa-miR-423-3p/mmu- HL
miR-423-3p/rno-miR-423
hsa-miR-526b HL
hsa-miR-526b* HL
ebv-miR-BART6-3p HL
hsa-miR-92b/mmu-miR- HL
92b/rno-miR-92b
hsa-miR-519e* HL
hiv1-miR-H1 HL
hsa-miR-623 HL
hsa-miR-483-5p HL
mghv-miR-M1-2 HL
mghv-miR-M1-7-3p HL
hsa-miR-519e HL
hsa-miR-361-5p/mmu- HL
miR-361/rno-miR-361
hsa-miR-650 HL
hsa-miR-361-3p HL
hsa-miR-374b* HL
kshv-miR-K12-8 HL
hsa-miR-150*/mmu-miR- HL
150*
hsa-miR-425*/mmu-miR- HL
425*
hsa-miR-135a*/mmu- HL
miR-135a*
hsa-miR-612 HL
hsa-miR-212/mmu-miR- HL
212/rno-miR-212
hsa-miR-125b-2*/rno- HL
miR-125b*
hcmv-miR-UL112 HL
hsa-miR-500 HL
hsa-miR-502-5p HL
ebv-miR-BART18-3p HL
hsa-miR-625 HL
hsa-miR-138/mmu-miR- HL
138/rno-miR-138
hsa-miR-500* HL
hsa-miR-124*/mmu-miR- HL
124*/rno-miR-124*
hsa-miR-516b HL
hsa-miR-30c-1*/mmu- HL
miR-30c-1*/rno-miR-
30c-1*
hsa-miR-331-5p/mmu- HL
miR-331-5p
hsa-miR-510 HL
hsa-miR-376a* HL
hsa-miR-640 HL
hsa-miR-331-3p/mmu- HL
miR-331-3p/rno-miR-331
hsa-miR-602 HL
hsa-miR-485-3p/mmu- HL
miR-485*
hsa-miR-488 HL
hsa-miR-125a- HL
3p/mmu-miR-125a-
3p/rno-miR-125a-3p
hsa-miR-24-2*/mmu- HL
miR-24-2*/rno-miR-
24-2*
hsa-miR-484/mmu- HL
miR-484/rno-miR-
484
hsa-miR-106b*/mmu- HL
miR-106b*/rno-miR-
106b*
hsa-miR-600 HL
hsa-let-7b*/mmu-let- HL
7b*/rno-let-7b*
hsa-miR-302c* HL
hsa-miR-20b* HL
hsa-miR-524-5p HL
hsa-miR-505* HL
hsa-miR-542- HL
5p/mmu-miR-542-
5p/rno-miR-542-5p
hsa-miR-557 HL
hsa-miR-183/mmu- HL
miR-183/rno-miR-
183
hsa-miR-122* HL
hsa-miR-675 HL
hsv1-miR-H1 HL
hsa-miR-99b/mmu- HL
miR-99b/rno-miR-
99b
hsa-miR-766 HL
hsa-miR-409- HL
5p/mmu-miR-409-
5p/rno-miR-409-5p
ebv-miR-BART20-3p HL
hsa-miR-129* HL
mghv-miR-M1-7-5p HL
hsa-miR-671- HL
5p/mmu-miR-671-5p
hsa-miR-629 HL
hsa-miR-553 HL
hsa-let-7d*/mmu-let- HL
7d*/rno-let-7d*
hsa-miR-601 HL
hsa-miR-645 HL
hsa-miR-221* HL
hsa-miR-874/mmu- HL
miR-874/rno-miR-
874
hsa-miR-890 HL
hsa-miR-492 HL
hsa-miR-629* HL
hsa-miR-635 HL
hsa-miR-130b/mmu- HL
miR-130b/rno-miR-
130b
hsa-miR-197/mmu- HL
miR-197
hsa-miR-654-5p HL
hsa-miR-518b HL
hsa-miR-889 HL
hsa-miR-584 HL
hsa-miR-198 HL
hsa-miR-636 HL
hsa-miR-630 HL
hsa-miR-490-5p HL
hsa-miR-663 HL
hcmv-miR-UL148D HL
hsa-miR-337-3p HL
hsa-miR-9*/mmu- HL
miR-9*/rno-miR-9*
hsa-miR-200b*/mmu- HL
miR-200b*
ebv-miR-BART9* HL
hsa-miR-342- HL
5p/mmu-miR-342-
5p/rno-miR-342-5p
hsa-miR-206/mmu- HL
miR-206/rno-miR-
206
hcmv-miR-US25-1* HL
hsa-miR-659 HL
hsa-miR-514 HL
kshv-miR-K12-6-5p HL
hsa-miR-508-5p HL
hsa-miR-377* HL
ebv-miR-BART16 HL
hsa-miR-181b/mmu- HL
miR-181b/rno-miR-
181b
hsa-miR-622 HL
kshv-miR-K12-1 HL
hsa-miR-490- HL
3p/mmu-miR-490
hsa-miR-125b- HL
1*/mmu-miR-125b-
3p/rno-miR-125b-3p
hsa-miR-124/mmu- HL
miR-124/rno-miR-
124
hsa-miR-657 HL
ebv-miR-BHRF1-3 HL
kshv-miR-K12-5 HL
hsa-miR-487b/mmu- HL
miR-487b/rno-miR-
487b
hsa-miR-183*/mmu- HL
miR-183*
hsa-miR-297/mmu- HL
miR-297a
hsa-miR-885-5p HL
hsa-miR-296- HL
3p/mmu-miR-296-
3p/rno-miR-296
ebv-miR-BART19-3p HL
hsa-miR-617 HL
hsa-miR-519d HL
hsa-miR-195* HL
hsa-miR-575 HL
hsa-miR-208a/mmu- HL
miR-208a/rno-miR-
208
hsa-miR-647 HL
hsa-miR-525-5p HL
ebv-miR-BART8* HL
hsa-miR-340*/mmu- HL
miR-340-3p/rno-miR-
340-3p
hsa-miR-220b HL
hsa-miR-382/mmu- HL
miR-382/rno-miR-
382
hsa-miR-585 HL
hsa-miR-877/mmu- HL
miR-877/rno-miR-
877
hsa-miR-99b*/mmu- HL
miR-99b*/rno-miR-
99b*
ebv-miR-BHRF1-1 HL
hsa-miR-326/mmu- HL
miR-326/rno-miR-
326
ebv-miR-BART7* HL
hsa-miR-615- HL
3p/mmu-miR-615-3p
mghv-miR-M1-8 HL
hsa-miR-193b* HL
ebv-miR-BART13 HL
hsa-miR-433/mmu- HL
miR-433/rno-miR-
433
hsa-miR-202 HL
hsa-miR-551b* HL
hsa-miR-551a HL
hsa-miR-542- HL
3p/mmu-miR-542-
3p/rno-miR-542-3p
hsa-miR-338- HL
5p/mmu-miR-338-
5p/rno-miR-338*
hsa-miR-299-3p HL
hsa-miR-518a-3p HL
hsa-miR-181a-2* HL
hsa-miR-938 HL
hsa-miR-509-3-5p HL
hsa-miR-552 HL
TABLE 21
Predictor microRNAs that distinguish germinal center B-cell like
(GCB) DLBCL from Burkitt lymphoma
GCBvsBL Higher in
hsa-miR-129* GCB
hsa-miR-28-5p/mmu-miR-28/rno- GCB
miR-28
hsa-miR-155 GCB
hsa-miR-196a*/mmu-miR-196a*/rno- GCB
miR-196a*
hsa-miR-146a/mmu-miR-146a/rno- GCB
miR-146a
hsa-miR-331-3p/mmu-miR-331- GCB
3p/rno-miR-331
hsa-miR-215 GCB
hsa-miR-600 GCB
mghv-miR-M1-7-3p GCB
hsa-miR-107/mmu-miR-107/rno- GCB
miR-107
hsa-miR-886-3p GCB
hsa-miR-140-5p/mmu-miR-140/rno- GCB
miR-140
hsa-miR-154/mmu-miR-154/rno- GCB
miR-154
hsa-miR-103/mmu-miR-103/rno- GCB
miR-103
hsa-let-7g/mmu-let-7g GCB
hsa-miR-222/mmu-miR-222/rno- GCB
miR-222
hsa-miR-221/mmu-miR-221/rno- GCB
miR-221
hsa-miR-320/mmu-miR-320/rno- GCB
miR-320
hsa-miR-140-3p/mmu-miR-140*/rno- GCB
miR-140*
hsa-miR-148a/mmu-miR-148a GCB
hsa-miR-365/mmu-miR-365/rno-miR- GCB
365
hsa-miR-29c/mmu-miR-29c/rno-miR- GCB
29c
hsa-miR-30d/mmu-miR-30d/rno-miR- GCB
30d
hsa-miR-214/mmu-miR-214/rno-miR- GCB
214
hsa-miR-146b-5p/mmu-miR-146b/rno- GCB
miR-146b
hsa-miR-342-3p/mmu-miR-342-3p/rno- GCB
miR-342-3p
hsa-miR-374a GCB
hsa-miR-223/mmu-miR-223/rno-miR- GCB
223
hsa-miR-29b/mmu-miR-29b/rno-miR- GCB
29b
hsa-miR-21/mmu-miR-21/rno-miR-21 GCB
hsa-miR-29a/mmu-miR-29a/rno-miR- GCB
29a
hsa-miR-23b/mmu-miR-23b/rno-miR- GCB
23b
hsa-miR-24/mmu-miR-24/rno-miR-24 GCB
hsa-miR-26a/mmu-miR-26a/rno-miR- GCB
26a
hsa-miR-26b/mmu-miR-26b/rno-miR- GCB
26b
hsa-miR-34b/mmu-miR-34b-3p GCB
hsa-miR-503 BL
hsa-miR-30b* BL
TABLE 22
Predictor microRNAs that distinguish germinal center B-cell like
(GCB) DLBCL from chronic lymphocytic leukemia
Higher
GCB vs CLL in
hsa-miR-181a/mmu- GCB
miR-181a/rno-miR-
181a
hsa-miR-886-5p GCB
mghv-miR-M1-7-3p GCB
hsa-miR-934 GCB
mghv-miR-M1-3 GCB
hsa-miR-485- GCB
3p/mmu-miR-485*
hsa-miR-125b/mmu- GCB
miR-125b-5p/rno-
miR-125b-5p
hsa-miR-637 GCB
hsa-miR-365/mmu- GCB
miR-365/rno-miR-
365
hsa-miR-505* GCB
hsa-miR-199a- GCB
3p/hsa-miR-199b-
3p/mmu-miR-199a-
3p/mmu-miR-
199b/rno-miR-199a-
3p
hsa-miR-675 GCB
hsa-miR-424 GCB
ebv-miR-BHRF1-2 GCB
hsa-miR-519c- GCB
5p/hsa-miR-519b-
5p/hsa-miR-
523*/hsa-miR-
518e*/hsa-miR-
522*/hsa-miR-
519a*
hsa-miR-130a/mmu- GCB
miR-130a/rno-miR-
130a
hsa-miR-943 GCB
hsa-miR-126/mmu- GCB
miR-126-3p/rno-
miR-126
hsa-miR-193b GCB
hsa-miR-198 GCB
hsa-miR- GCB
200b*/mmu-miR-
200b*
kshv-miR-K12-6-3p GCB
hsa-miR-220c GCB
hsa-miR-374b* GCB
hsa-miR-518b GCB
hsa-miR-920 GCB
hsa-miR-125b- GCB
1*/mmu-miR-125b-
3p/rno-miR-125b-3p
ebv-miR-BART8* GCB
ebv-miR-BART16 GCB
hsa-miR-630 GCB
hsa-miR-483-5p GCB
hsa-miR-422a GCB
hsa-miR-526b GCB
hsa-miR-145/mmu- GCB
miR-145/rno-miR-
145
hsa-miR-126*/mmu- GCB
miR-126-5p/rno-
miR-126*
hsa-miR-143/mmu- GCB
miR-143/rno-miR-
143
hsa-miR-100/mmu- GCB
miR-100/rno-miR-100
hsa-miR-371-5p GCB
hsa-miR-193a-5p GCB
hsa-miR-628-3p GCB
hsa-miR-185* GCB
hsa-miR-10b/mmu- GCB
miR-10b/rno-miR-10b
hsa-miR-665 GCB
hsa-miR-503 GCB
hsa-miR-642 GCB
hsa-miR-658 GCB
hsa-miR-21/mmu- GCB
miR-21/rno-miR-21
hsa-miR-23a/mmu- GCB
miR-23a/rno-miR-23a
hsa-miR-125a- GCB
5p/mmu-miR-125a-
5p/rno-miR-125a-5p
hsa-miR-24/mmu- GCB
miR-24/rno-miR-24
hsa-miR-23b/mmu- GCB
miR-23b/rno-miR-23b
hsa-miR-620 GCB
hsa-miR-933 GCB
hsa-miR-30b* GCB
hsa-miR-22/mmu- GCB
miR-22/rno-miR-22
hsa-let-7e/mmu-let- GCB
7e/rno-let-7e
mghv-miR-M1-4 GCB
hsa-miR-149* GCB
hsa-miR-765 GCB
hsa-let-7c/mmu-let- GCB
7c/rno-let-7c
hsa-miR-423- CLL
5p/mmu-miR-423-5p
hsa-miR-32* CLL
hsa-miR-34b/mmu- CLL
miR-34b-3p
hsa-miR-551b/mmu- CLL
miR-551b/rno-miR-
551b
hsa-let-7i/mmu-let- CLL
7i/rno-let-7i
hsa-miR-29a/mmu- CLL
miR-29a/rno-miR-29a
hsa-miR-138- CLL
1*/mmu-miR-
138*/rno-miR-138*
hsa-miR-29b/mmu- CLL
miR-29b/rno-miR-29b
hsa-miR-191/mmu- CLL
miR-191/rno-miR-191
hsa-miR-26b/mmu- CLL
miR-26b/rno-miR-26b
hsa-miR-26a/mmu- CLL
miR-26a/rno-miR-26a
hsa-miR-550 CLL
hsa-miR-374a CLL
hsa-miR-142-5p/mmu- CLL
miR-142-5p/rno-miR-
142-5p
hsa-miR-29c/mmu- CLL
miR-29c/rno-miR-29c
hsa-miR-140-3p/mmu- CLL
miR-140*/rno-miR-
140*
hsa-miR-30e/mmu- CLL
miR-30e/rno-miR-30e
hsa-miR-801/mmu- CLL
miR-801
hsa-miR-768-3p CLL
hsa-miR-549 CLL
hsa-miR-199a-5p/mmu- CLL
miR-199a-5p/rno-miR-
199a-5p
hsa-miR-223/mmu- CLL
miR-223/rno-miR-223
hsa-miR-101/mmu- CLL
miR-101a/rno-miR-
101a
hsa-miR-888* CLL
hsa-miR-24-1*/mmu- CLL
miR-24-1*/rno-miR-24-
1*
hsa-miR-519d CLL
hsa-miR-154/mmu- CLL
miR-154/rno-miR-154
hsa-miR-638 CLL
hsa-miR-668/mmu- CLL
miR-668
hsa-miR-891a CLL
hsa-miR-768-5p CLL
hsa-miR-140-5p/mmu- CLL
miR-140/rno-miR-140
hsa-miR-196a*/mmu- CLL
miR-196a*/rno-miR-
196a*
hsa-miR-150/mmu- CLL
miR-150/rno-miR-150
hsa-let-7g/mmu-let-7g CLL
hsa-miR-363/mmu- CLL
miR-363/rno-miR-363
hsa-miR-486-5p/mmu- CLL
miR-486
hsa-miR-32/mmu-miR- CLL
32/rno-miR-32
hsa-miR-147 CLL
hsa-miR-20b* CLL
hsa-miR-487b/mmu- CLL
miR-487b/rno-miR-
487b
hsa-miR-636 CLL
hsa-miR-144* CLL
hsa-miR-186/mmu- CLL
miR-186/rno-miR-186
hsa-miR-30e*/mmu- CLL
miR-30e*/rno-miR-
30e*
hsa-miR-331-5p/mmu- CLL
miR-331-5p
TABLE 23
Predictor microRNAs that distinguish germinal center B-cell like
(GCB) DLBCL from follicular lymphoma
Higher
GCB vs FL in
hsa-miR-378/mmu- GCB
miR-378/rno-miR-378
hsa-miR-20b/mmu- GCB
miR-20b/rno-miR-20b-
5p
hsa-miR-19b/mmu- GCB
miR-19b/rno-miR-19b
hsa-miR-106a GCB
hsa-miR-17/mmu-miR- GCB
17/rno-miR-17-5p/rno-
miR-17
hsa-miR-93/mmu-miR- GCB
93/rno-miR-93
hsa-miR-20a/mmu- GCB
miR-20a/rno-miR-20a
hsa-miR-23b/mmu- GCB
miR-23b/rno-miR-23b
hsa-miR-23a/mmu- GCB
miR-23a/rno-miR-23a
hsa-miR-22/mmu-miR- GCB
22/rno-miR-22
hsa-miR-19a/mmu- GCB
miR-19a/rno-miR-19a
hsa-miR-320/mmu- GCB
miR-320/rno-miR-320
hsa-miR-106b/mmu- GCB
miR-106b/rno-miR-
106b
hsa-miR-103/mmu- GCB
miR-103/rno-miR-103
hsa-miR-30c/mmu- GCB
miR-30c/rno-miR-30c
ebv-miR-BHRF1-2 GCB
hsa-miR-125a- GCB
5p/mmu-miR-125a-
5p/rno-miR-125a-5p
hsa-let-7a/mmu-let- GCB
7a/rno-let-7a
hsa-miR-628-3p GCB
hsa-let-7c/mmu-let- GCB
7c/rno-let-7c
hsa-miR-423-5p/mmu- FL
miR-423-5p
hsa-miR-24-1*/mmu- FL
miR-24-1*/rno-miR-
24-1*
ebv-miR-BART2-3p FL
hsa-miR-138-1*/mmu- FL
miR-138*/rno-miR-
138*
hsa-miR-768-5p FL
hsa-miR-30b* FL
hsa-miR-494/mmu- FL
miR-494/rno-miR-494
hsa-miR-583 FL
hsa-miR-185/mmu- FL
miR-185/rno-miR-185
hsa-miR-765 FL
hsa-miR-34b/mmu- FL
miR-34b-3p
hsa-miR-921 FL
hsa-miR-551b/mmu- FL
miR-551b/rno-miR-
551b
hsa-miR-549 FL
hsa-miR-939 FL
hsa-miR-302d* FL
ebv-miR-BART17-5p FL
hsa-miR-801/mmu- FL
miR-801
hsa-miR-888* FL
hsa-miR-620 FL
hsa-miR-576-3p FL
hsa-miR-32* FL
hsa-miR-574-5p/mmu- FL
miR-574-5p
hsa-miR-505/rno-miR- FL
505
hsa-miR-885-5p FL
hsa-miR-455-3p FL
hsa-miR-152/mmu- FL
miR-152/rno-miR-152
hsa-miR-200b*/mmu- FL
miR-200b*
mghv-miR-M1-2 FL
hcmv-miR-UL148D FL
hsa-miR-497/mmu-miR- FL
497/rno-miR-497
ebv-miR-BART13 FL
ebv-miR-BART16 FL
hsa-miR-125b-2*/rno- FL
miR-125b*
hsa-miR-490-3p/mmu- FL
miR-490
hsa-miR-99b/mmu-miR- FL
99b/rno-miR-99b
hsa-miR-339-5p/mmu- FL
miR-339-5p/rno-miR-
339-5p
hsa-miR-574-3p/mmu- FL
miR-574-3p
hsa-miR-515-5p FL
hsa-miR-877/mmu-miR- FL
877/rno-miR-877
hsa-miR-208a/mmu-miR- FL
208a/rno-miR-208
hcmv-miR-US25-1* FL
hsa-miR-326/mmu-miR- FL
326/rno-miR-326
hsa-miR-488 FL
hsa-miR-629 FL
hsa-miR-24-2*/mmu- FL
miR-24-2*/rno-miR-24-
2*
hsa-miR-124/mmu-miR- FL
124/rno-miR-124
hsa-miR-493 FL
hsv1-miR-H1 FL
hsa-miR-484/mmu-miR- FL
484/rno-miR-484
hsa-miR-483-3p FL
hsa-miR-27a*/mmu-miR- FL
27a*/rno-miR-27a*
hsa-miR-144* FL
hsa-miR-617 FL
hsa-miR-377* FL
hsa-miR-363*/rno-miR- FL
363*
hsa-miR-148b/mmu-miR- FL
148b/rno-miR-148b-3p
kshv-miR-K12-1 FL
hsa-miR-7/mmu-miR- FL
7a/rno-miR-7a
hsa-miR-193b* FL
hsa-miR-542-3p/mmu- FL
miR-542-3p/rno-miR-
542-3p
hsa-miR-601 FL
hsa-miR-106b*/mmu- FL
miR-106b*/rno-miR- FL
106b*
hsa-miR-7-2* FL
kshv-miR-K12-6-5p FL
hsa-miR-645 FL
hsa-miR-524-5p FL
hsa-miR-548d-5p FL
hsa-miR-9*/mmu-miR- FL
9*/rno-miR-9*
hsa-miR-92a/mmu-miR- FL
92a/rno-miR-92a
hsa-miR-22*/mmu-miR- FL
22*/rno-miR-22*
hsa-miR-500* FL
hsa-miR-890 FL
hsa-miR-297/mmu-miR- FL
297a
hsa-miR-197/mmu-miR- FL
197
hsa-miR-20b* FL
hsa-miR-629* FL
hsa-miR-887 FL
hsa-miR-425/mmu- FL
miR-425/rno-miR-425
hsa-miR-99b*/mmu- FL
miR-99b*/rno-miR-
99b*
hsa-miR-513a-3p FL
hsa-miR-206/mmu- FL
miR-206/rno-miR-206
hsa-miR-155* FL
hsa-miR-181b/mmu- FL
miR-181b/rno-miR-
181b
hsa-miR-299-3p FL
hsa-miR-218-2*/mmu- FL
miR-218-2*/rno-miR-
218*
mghv-miR-M1-8 FL
ebv-miR-BART18-3p FL
hsa-miR-708/mmu- FL
miR-708/rno-miR-708
hsa-miR-409-5p/mmu- FL
miR-409-5p/rno-miR-
409-5p
hsa-miR-553 FL
hsa-miR-361-3p FL
hsa-miR-296-3p/mmu- FL
miR-296-3p/rno-miR-
296
ebv-miR-BHRF1-3 FL
hsa-miR-34c-5p/mmu- FL
miR-34c/rno-miR-34c
hsa-let-7b*/mmu-let- FL
7b*/rno-let-7b*
hsa-miR-301a/mmu- FL
miR-301a/rno-miR-
301a
hsa-miR-122* FL
hsa-miR-183/mmu- FL
miR-183/rno-miR-183
kshv-miR-K12-5 FL
kshv-miR-K12-7 FL
hsa-miR-552 FL
hsa-miR-151-3p FL
hsa-miR-194/mmu- FL
miR-194/rno-miR-194
hsa-miR-585 FL
hsa-miR-340*/mmu- FL
miR-340-3p/rno-miR-
340-3p
hsa-let-7d*/mmu-let- FL
7d*/rno-let-7d*
hsa-miR-622 FL
hsa-miR-575 FL
hsa-miR-514 FL
hsa-miR-92b/mmu- FL
miR-92b/rno-miR-92b
hsa-miR-551a FL
hsa-miR-221* FL
hsa-miR-922 FL
hsa-miR-938 FL
hsa-miR-615-3p/mmu- FL
miR-615-3p
hsa-miR-220b FL
hsa-miR-744/mmu- FL
miR-744
hsa-miR-657 FL
hsa-miR-382/mmu- FL
miR-382/rno-miR-382
hsa-miR-518a-3p FL
hsa-miR-138/mmu- FL
miR-138/rno-miR-138
hsa-miR-636 FL
hsa-miR-96/mmu- FL
miR-96/rno-miR-96
hsa-miR-509-3-5p FL
hsa-miR-337-3p FL
hsa-miR-342-5p/mmu- FL
miR-342-5p/rno-miR-
342-5p
TABLE 24
Predictor microRNAs that distinguish germinal center B-cell like
(GCB) DLBCL from Hodgkin's lymphoma
Higher
GCB vs HL in
hsa-miR-19b/mmu-miR- GCB
19b/rno-miR-19b
hsa-miR-19a/mmu-miR- GCB
19a/rno-miR-19a
hsa-miR-106a GCB
hsa-miR-20b/mmu-miR- GCB
20b/rno-miR-20b-5p
hsa-miR-17/mmu-miR- GCB
17/rno-miR-17-5p/rno-
miR-17
hsa-miR-15b/mmu-miR- GCB
15b/rno-miR-15b
hsa-miR-20a/mmu-miR- GCB
20a/rno-miR-20a
hsa-miR-30b/mmu-miR- GCB
30b/rno-miR-30b-5p
hsa-miR-142-3p/mmu-miR- GCB
142-3p/rno-miR-142-3p
hsa-miR-30c/mmu-miR- GCB
30c/rno-miR-30c
hsa-miR-378/mmu-miR- GCB
378/rno-miR-378
hsa-miR-93/mmu-miR- GCB
93/rno-miR-93
hsa-miR-106b/mmu-miR- GCB
106b/rno-miR-106b
hsa-miR-374a GCB
hsa-miR-24/mmu-miR- GCB
24/rno-miR-24
hsa-miR-29b/mmu-miR- GCB
29b/rno-miR-29b
hsa-miR-22/mmu-miR- GCB
22/rno-miR-22
hsa-miR-142-5p/mmu-miR- GCB
142-5p/rno-miR-142-5p
hsa-miR-30e/mmu-miR- GCB
30e/rno-miR-30e
hsa-miR-30a/mmu-miR- GCB
30a/rno-miR-30a
hsa-miR-30d/mmu-miR- GCB
30d/rno-miR-30d
hsa-miR-23b/mmu-miR- GCB
23b/rno-miR-23b
hsa-miR-16/mmu-miR- GCB
16/rno-miR-16
hsa-miR-191/mmu-miR- GCB
191/rno-miR-191
hsa-miR-15a/mmu-miR- GCB
15a
hsa-miR-26b/mmu-miR- GCB
26b/rno-miR-26b
hsa-miR-23a/mmu-miR- GCB
23a/rno-miR-23a
hsa-let-7a/mmu-let-7a/rno- GCB
let-7a
hsa-miR-103/mmu-miR- GCB
103/rno-miR-103
hsa-miR-140-3p/mmu-miR- GCB
140*/rno-miR-140*
hsa-miR-154/mmu-miR- GCB
154/rno-miR-154
hsa-miR-320/mmu-miR- GCB
320/rno-miR-320
hsa-miR-550 GCB
hsa-miR-125a-5p/mmu- GCB
miR-125a-5p/rno-miR-
125a-5p
hsa-let-7c/mmu-let-7c/rno- GCB
let-7c
hsa-miR-185/mmu-miR- HL
185/rno-miR-185
hsa-miR-658 HL
hsa-miR-549 HL
hsa-miR-634 HL
hsa-miR-551b/mmu-miR- HL
551b/rno-miR-551b
hsa-miR-518c* HL
hsa-miR-888* HL
hsa-miR-765 HL
hsa-miR-423-5p/mmu-miR- HL
423-5p
hsa-miR-30c-2*/mmu-miR- HL
30c-2*/rno-miR-30c-2*
hsa-miR-503 HL
hsa-miR-921 HL
hsa-miR-520d-5p HL
hsa-miR-574-5p/mmu-miR- HL
574-5p
hsa-miR-32* HL
hsa-miR-939 HL
ebv-miR-BART2-3p HL
ebv-miR-BHRF1-2 HL
hsa-miR-583 HL
hsa-miR-30b* HL
hsa-miR-149* HL
mghv-miR-M1-4 HL
hsa-miR-513a-5p HL
hsa-miR-494/mmu-miR- HL
494/rno-miR-494
hsa-miR-498 HL
hsa-miR-485-3p/mmu-miR- HL
485*
hsa-miR-129-5p/mmu-miR- HL
129-5p/rno-miR-129
hsa-miR-25* HL
hsa-miR-923 HL
hsa-miR-519d HL
hsa-miR-516a-5p HL
hsa-miR-99a/mmu-miR- HL
99a/rno-miR-99a
hsa-miR-943 HL
hsa-miR-885-5p HL
ebv-miR-BHRF1-1 HL
hsa-miR-152/mmu-miR- HL
152/rno-miR-152
ebv-miR-BART8* HL
hsa-miR-200b*/mmu-miR- HL
200b*
hsa-miR-125b-1*/mmu- HL
miR-125b-3p/rno-miR-
125b-3p
hsa-miR-526b HL
hsa-miR-29a*/mmu-miR- HL
29a*/rno-miR-29a*
hsa-miR-532-5p/mmu-miR- HL
532-5p/rno-miR-532-5p
hsa-miR-183*/mmu-miR- HL
183*
hsa-miR-659 HL
hsa-miR-145*/mmu-miR- HL
145*
mghv-miR-M1-7-5p HL
hsa-miR-143* HL
hsa-miR-660 HL
hsa-miR-130b/mmu- HL
miR-130b/rno-miR-130b
hsa-miR-671-5p/mmu- HL
miR-671-5p
hsa-miR-525-5p HL
hsa-miR-505/rno-miR- HL
505
hsa-miR-488 HL
hsa-miR-766 HL
hsa-miR-20b* HL
hsa-miR-339-5p/mmu- HL
miR-339-5p/rno-miR-
339-5p
hsa-miR-524-5p HL
hsa-miR-455-3p HL
hsa-miR-92a/mmu-miR- HL
92a/rno-miR-92a
hsa-miR-502-3p HL
hsa-miR-210/mmu-miR- HL
210/rno-miR-210
hsv1-miR-H1 HL
ebv-miR-BART6-3p HL
hsa-miR-490-3p/mmu- HL
miR-490
hsa-miR-149/mmu-miR- HL
149
hsa-miR-128/mmu-miR- HL
128/rno-miR-128
hsa-miR-635 HL
hcmv-miR-UL148D HL
hsa-miR-373* HL
hsa-miR-647 HL
hsa-miR-197/mmu-miR- HL
197
hsa-miR-602 HL
hsa-miR-656 HL
hsa-miR-874/mmu-miR- HL
874/rno-miR-874
ebv-miR-BART19-3p HL
hsa-miR-551b* HL
hsa-miR-96/mmu-miR- HL
96/rno-miR-96
hsa-miR-889 HL
hsa-miR-425/mmu-miR- HL
425/rno-miR-425
hsa-miR-34c-5p/mmu- HL
miR-34c/rno-miR-34c
hcmv-miR-UL70-3p HL
hsa-miR-27a*/mmu- HL
miR-27a*/rno-miR-27a*
hsa-miR-194/mmu-miR- HL
194/rno-miR-194
hsa-miR-17*/rno-miR- HL
17-3p
hsa-miR-548d-5p HL
hsa-miR-7/mmu-miR- HL
7a/rno-miR-7a
hsa-miR-877/mmu-miR- HL
877/rno-miR-877
hsa-miR-22*/mmu-miR- HL
22*/rno-miR-22*
hsa-miR-323-3p/mmu- HL
miR-323-3p/rno-miR-
323
hsa-miR-708/mmu-miR- HL
708/rno-miR-708
hsa-miR-513a-3p HL
hsa-miR-595 HL
hsa-miR-922 HL
hsa-miR-515-5p HL
hsa-miR-99b/mmu-miR- HL
99b/rno-miR-99b
hsa-miR-483-3p HL
hsa-miR-330-5p/mmu- HL
miR-330/rno-miR-330
hsa-miR-509-3p HL
hsa-miR-151-3p HL
ebv-miR-BART13 HL
hsa-miR-617 HL
hsa-miR-328/mmu-miR- HL
328/rno-miR-328
hsa-miR-361-3p HL
hsa-miR-138/mmu-miR- HL
138/rno-miR-138
ebv-miR-BART7* HL
hsa-miR-589 HL
hsa-miR-576-5p HL
hsa-miR-452 HL
hsa-miR-7-2* HL
hsa-miR-296-5p/mmu- HL
miR-296-5p/rno-miR-
296*
hsa-miR-550* HL
hsa-miR-92b/mmu-miR- HL
92b/rno-miR-92b
kshv-miR-K12-1 HL
hsa-miR-526a/hsa-miR- HL
520c-5p/hsa-miR-518d-
5p
mghv-miR-M1-2 HL
hsa-miR-548b-3p HL
hsa-miR-297/mmu-miR- HL
297a
hsa-miR-195* HL
hsa-miR-652/mmu-miR- HL
652/rno-miR-652
hsa-miR-221* HL
hsa-miR-194* HL
hsa-miR-23a*/rno-miR- HL
23a*
hsa-miR-125b-2*/rno- HL
miR-125b*
hsa-miR-212/mmu-miR- HL
212/rno-miR-212
ebv-miR-BART18-3p HL
hsa-miR-586 HL
hsa-miR-137/mmu-miR- HL
137/rno-miR-137
hsa-miR-610 HL
hcmv-miR-UL112 HL
hsa-miR-181b/mmu- HL
miR-181b/rno-miR-181b
hsa-miR-936 HL
hsa-miR-744/mmu- HL
miR-744
kshv-miR-K12-6-5p HL
hcmv-miR-US25-1* HL
hsa-miR-21* HL
mghv-miR-M1-6 HL
mghv-miR-M1-8 HL
hsa-miR-10a*/mmu- HL
miR-10a*/rno-miR-
10a-3p
hsa-miR-345 HL
hsa-miR-887 HL
hsa-miR-193b* HL
hsa-miR-122* HL
kshv-miR-K12-5 HL
hsa-miR-92b* HL
hsa-miR-526b* HL
hsa-miR-553 HL
hsa-miR-601 HL
hiv1-miR-H1 HL
hsa-miR-623 HL
hsa-miR-519e HL
hsa-miR-650 HL
hsa-miR-575 HL
hsa-miR-629* HL
hsa-miR-890 HL
hsa-miR- HL
150*/mmu-miR-
150*
hsa-miR- HL
425*/mmu-miR-
425*
hsa-miR- HL
135a*/mmu-miR-
135a*
hsa-miR-612 HL
hsa-miR-636 HL
hsa-miR-500 HL
hsa-miR-502-5p HL
hsa-miR-9*/mmu- HL
miR-9*/rno-miR-9*
hsa-miR-500* HL
hsa-miR- HL
124*/mmu-miR-
124*/rno-miR-124*
hsa-miR-30c- HL
1*/mmu-miR-30c-
1*/rno-miR-30c-1*
hsa-miR- HL
99b*/mmu-miR-
99b*/rno-miR-99b*
hsa-miR-331- HL
5p/mmu-miR-331-
5p
hsa-miR-206/mmu- HL
miR-206/rno-miR-
206
hsa-miR-376a* HL
hsa-miR-585 HL
hsa-miR-640 HL
hsa-miR-377* HL
hsa-miR-125a- HL
3p/mmu-miR-125a-
3p/rno-miR-125a-3p
hsa-miR-24- HL
2*/mmu-miR-24-
2*/rno-miR-24-2*
hsa-miR-484/mmu- HL
miR-484/rno-miR-
484
hsa-miR- HL
106b*/mmu-miR-
106b*/rno-miR-
106b*
hsa-let-7b*/mmu- HL
let-7b*/rno-let-7b*
hsa-miR-302c* HL
hsa-miR-542- HL
5p/mmu-miR-542-
5p/rno-miR-542-5p
hsa-miR-622 HL
ebv-miR-BHRF1-3 HL
hsa-miR-181a-2* HL
hsa-miR-183/mmu- HL
miR-183/rno-miR-
183
hsa-miR-409- HL
5p/mmu-miR-409-
5p/rno-miR-409-5p
ebv-miR-BART20- HL
3p
hsa-miR-629 HL
hsa-let-7d*/mmu- HL
let-7d*/rno-let-7d*
hsa-miR-645 HL
hsa-miR-492 HL
hsa-miR-654-5p HL
hsa-miR-208a/mmu- HL
miR-208a/rno-miR-
208
hsa-miR-584 HL
hsa-miR-382/mmu- HL
miR-382/rno-miR-
382
hsa-miR- HL
340*/mmu-miR-
340-3p/rno-miR-
340-3p
hsa-miR-490-5p HL
hsa-miR-663 HL
hsa-miR-337-3p HL
hsa-miR-518a-3p HL
ebv-miR-BART9* HL
hsa-miR-342- HL
5p/mmu-miR-342-
5p/rno-miR-342-5p
hsa-miR-514 HL
hsa-miR-508-5p HL
hsa-miR-124/mmu- HL
miR-124/rno-miR-
124
hsa-miR-657 HL
hsa-miR-938 HL
hsa-miR-296- HL
3p/mmu-miR-296-
3p/rno-miR-296
hsa-miR-551a HL
hsa-miR-542- HL
3p/mmu-miR-542-
3p/rno-miR-542-3p
hsa-miR-220b HL
hsa-miR-326/mmu- HL
miR-326/rno-miR-
326
hsa-miR-615- HL
3p/mmu-miR-615-
3p
hsa-miR-433/mmu- HL
miR-433/rno-miR-
433
hsa-miR-202 HL
hsa-miR-338- HL
5p/mmu-miR-338-
5p/rno-miR-338*
hsa-miR-552 HL
hsa-miR-299-3p HL
hsa-miR-509-3-5p HL
TABLE 25
Predictor microRNAs that distinguish Burkitt lymphoma
from chromic lymphocytic leukemia
Higher
BL vs CLL in
hsa-miR-874/mmu- BL
miR-874/rno-miR-874
hsa-miR-125b/mmu- BL
miR-125b-5p/rno-miR-
125b-5p
hsa-miR-126/mmu- BL
miR-126-3p/rno-miR-
126
ebv-miR-BHRF1-2 BL
hsa-miR-193b BL
hsa-miR-371-5p BL
hsa-miR-193a-5p BL
hsa-miR-628-3p BL
hsa-miR-185* BL
hsa-miR-503 BL
hsa-miR-199a-3p/hsa- BL
miR-199b-3p/mmu-
miR-199a-3p/mmu-
miR-199b/rno-miR-
199a-3p
hsa-miR-143/mmu- BL
miR-143/rno-miR-143
hsa-miR-130a/mmu- BL
miR-130a/rno-miR-
130a
hsa-miR-145/mmu- BL
miR-145/rno-miR-145
hsa-miR-30b* BL
hsa-miR-665 BL
hsa-miR-658 BL
hsa-miR-933 BL
hsa-miR-30c-2*/mmu- BL
miR-30c-2*/rno-miR-
30c-2*
hsa-miR-765 BL
hsa-miR-620 BL
hsa-miR-520d-5p BL
hsa-miR-494/mmu- BL
miR-494/rno-miR-494
hsa-miR-551b/mmu- CLL
miR-551b/rno-miR-
551b
hsa-miR-106b/mmu- CLL
miR-106b/rno-miR-
106b
hsa-miR-30c/mmu- CLL
miR-30c/rno-miR-30c
hsa-miR-16/mmu-miR- CLL
16/rno-miR-16
hsa-miR-27a/mmu- CLL
miR-27a/rno-miR-27a
hsa-miR-27b/mmu- CLL
miR-27b/rno-miR-27b
hsa-miR-550 CLL
hsa-miR-30a/mmu- CLL
miR-30a/rno-miR-30a
hsa-miR-30b/mmu-miR- CLL
30b/rno-miR-30b-5p
hsa-miR-34b/mmu-miR- CLL
34b-3p
hsa-miR-801/mmu-miR- CLL
801
hsa-miR-26b/mmu-miR- CLL
26b/rno-miR-26b
hsa-let-7b/mmu-let- CLL
7b/rno-let-7b
hsa-miR-142-5p/mmu- CLL
miR-142-5p/rno-miR-
142-5p
hsa-miR-26a/mmu-miR- CLL
26a/rno-miR-26a
hsa-miR-29a/mmu-miR- CLL
29a/rno-miR-29a
hsa-miR-768-3p CLL
hsa-miR-199a-5p/mmu- CLL
miR-199a-5p/rno-miR-
199a-5p
hsa-miR-30e/mmu-miR- CLL
30e/rno-miR-30e
hsa-miR-29b/mmu-miR- CLL
29b/rno-miR-29b
hsa-miR-101/mmu-miR- CLL
101a/rno-miR-101a
hsa-miR-138-1*/mmu- CLL
miR-138*/rno-miR-138*
hsa-miR-195/mmu-miR- CLL
195/rno-miR-195
hsa-miR-549 CLL
hsa-miR-103/mmu-miR- CLL
103/rno-miR-103
hsa-miR-649 CLL
hsa-miR-335/mmu-miR- CLL
335-5p/rno-miR-335
hsa-miR-342-3p/mmu- CLL
miR-342-3p/rno-miR-
342-3p
hsa-miR-423-3p/mmu- CLL
miR-423-3p/rno-miR-
423
hsa-miR-222/mmu-miR- CLL
222/rno-miR-222
hsa-miR-374a CLL
hsa-miR-888* CLL
hsa-miR-30d/mmu-miR- CLL
30d/rno-miR-30d
hsa-miR-299-5p/mmu- CLL
miR-299*/rno-miR-299
hsa-miR-107/mmu-miR- CLL
107/rno-miR-107
hsa-miR-105 CLL
hsa-let-7f/mmu-let- CLL
7f/rno-let-7f
hsa-miR-191/mmu-miR- CLL
191/rno-miR-191
hsa-miR-223/mmu-miR- CLL
223/rno-miR-223
hsa-miR-361-5p/mmu- CLL
miR-361/rno-miR-361
hsa-miR-29c/mmu- CLL
miR-29c/rno-miR-29c
hsa-miR-147 CLL
hsa-miR-361-3p CLL
hsa-miR-140-3p/mmu- CLL
miR-140*/rno-miR-
140*
hsa-miR-486-5p/mmu- CLL
miR-486
hsa-miR-33a/mmu- CLL
miR-33/rno-miR-33
hsa-miR-636 CLL
hsa-miR-24-1*/mmu- CLL
miR-24-1*/rno-miR-24-
1*
hsa-miR-144* CLL
hsa-miR-668/mmu- CLL
miR-668
hsa-miR-768-5p CLL
hsa-miR-363/mmu- CLL
miR-363/rno-miR-363
hsa-miR-150/mmu- CLL
miR-150/rno-miR-150
hsa-miR-519d CLL
hsa-miR-891a CLL
hsa-miR-186/mmu- CLL
miR-186/rno-miR-186
hsa-miR-331-5p/mmu- CLL
miR-331-5p
hsa-miR-28-5p/mmu- CLL
miR-28/rno-miR-28
hsa-miR-154/mmu- CLL
miR-154/rno-miR-154
hsa-miR-155 CLL
hsa-miR-363*/rno-miR- CLL
363*
hsa-miR-32/mmu-miR- CLL
32/rno-miR-32
hsa-miR-30e*/mmu- CLL
miR-30e*/rno-miR-
30e*
hsa-miR-140-5p/mmu- CLL
miR-140/rno-miR-140
hsa-let-7g/mmu-let-7g CLL
hsa-miR-20b* CLL
hsa-miR-129* CLL
hsa-miR-196a*/mmu- CLL
miR-196a*/rno-miR-
196a*
hsa-miR-487b/mmu- CLL
miR-487b/rno-miR-
487b
TABLE 26
Predictor microRNAs that distinguish Burkitt lymphoma
from follicular lymphoma
Higher
BL vs FL in
hsa-miR-17/mmu-miR- BL
17/rno-miR-17-5p/rno-
miR-17
hsa-miR-106a BL
hsa-miR-19b/mmu-miR- BL
19b/rno-miR-19b
hsa-miR-20a/mmu-miR- BL
20a/rno-miR-20a
hsa-miR-19a/mmu-miR- BL
19a/rno-miR-19a
hsa-miR-628-3p BL
hsa-miR-503 BL
hsa-miR-371-5p BL
hsa-miR-106b/mmu- BL
miR-106b/rno-miR-106b
hsa-miR-30c-2*/mmu- BL
miR-30c-2*/rno-miR-
30c-2*
ebv-miR-BART2-3p FL
hsa-let-7e/mmu-let- FL
7e/rno-let-7e
hsa-miR-551b/mmu- FL
miR-551b/rno-miR-551b
hsa-miR-26b/mmu-miR- FL
26b/rno-miR-26b
hsa-miR-26a/mmu-miR- FL
26a/rno-miR-26a
hsa-miR-620 FL
hsa-miR-801/mmu-miR- FL
801
ebv-miR-BART17-5p FL
hsa-miR-29a/mmu-miR- FL
29a/rno-miR-29a
hsa-miR-34b/mmu-miR- FL
34b-3p
hsa-miR-32* FL
hsa-miR-29b/mmu-miR- FL
29b/rno-miR-29b
hsa-miR-649 FL
hsa-miR-576-3p FL
hsa-miR-302a/mmu- FL
miR-302a
hsa-miR-365/mmu-miR- FL
365/rno-miR-365
hsa-miR-148a/mmu- FL
miR-148a
hsa-miR-146b-5p/mmu- FL
miR-146b/rno-miR-146b
hsa-miR-505/rno-miR- FL
505
hsa-miR-33a/mmu-miR- FL
33/rno-miR-33
hsa-miR-455-3p FL
hsa-miR-374b/mmu- FL
miR-374/rno-miR-374
hsa-miR-214/mmu-miR- FL
214/rno-miR-214
hsa-miR-138-1*/mmu- FL
miR-138*/rno-miR-138*
hsa-miR-140-3p/mmu- FL
miR-140*/rno-miR-140*
hsa-miR-212/mmu-miR- FL
212/rno-miR-212
hsa-miR-29c/mmu-miR- FL
29c/rno-miR-29c
hsa-miR-888* FL
hsa-miR-222/mmu-miR- FL
222/rno-miR-222
hsa-miR-152/mmu-miR- FL
152/rno-miR-152
hsa-miR-183*/mmu- FL
miR-183*
hsa-miR-768-5p FL
hsa-miR-107/mmu-miR- FL
107/rno-miR-107
hsa-miR-574-5p/mmu- FL
miR-574-5p
hsa-miR-154/mmu-miR- FL
154/rno-miR-154
hsa-miR-620 FL
hsa-miR-886-5p FL
hsa-miR-208a/mmu- FL
miR-208a/rno-miR-208
hsa-miR-374b* FL
hsa-miR-525-5p FL
hsa-miR-363/mmu-miR- FL
363/rno-miR-363
hsa-miR-99b/mmu-miR- FL
99b/rno-miR-99b
hsa-miR-148b/mmu- FL
miR-148b/rno-miR-
148b-3p
kshv-miR-K12-6-5p FL
hsa-miR-125b-1*/mmu- FL
miR-125b-3p/rno-miR-
125b-3p
hsa-miR-526b FL
hsa-miR-629 FL
hsa-miR-617 FL
hsa-miR-124/mmu-miR- FL
124/rno-miR-124
hsa-miR-493 FL
hsa-miR-24-1*/mmu- FL
miR-24-1*/rno-miR-
24-1*
hsa-miR-200b*/mmu- FL
miR-200b*
hsa-miR-484/mmu- FL
miR-484/rno-miR-484
hsa-miR-483-3p FL
hsa-miR-516b FL
hsa-miR-125b-2*/rno- FL
miR-125b*
hsa-miR-490-3p/mmu- FL
miR-490
hsa-miR-140-5p/mmu- FL
miR-140/rno-miR-140
hsa-miR-877/mmu- FL
miR-877/rno-miR-877
hsa-miR-381/mmu- FL
miR-381/rno-miR-381
hsa-miR-193b* FL
hsa-miR-635 FL
hsa-miR-542-3p/mmu- FL
miR-542-3p/rno-miR-
542-3p
hsa-miR-181a-2* FL
hsa-miR-32/mmu- FL
miR-32/rno-miR-32
hsa-miR-105 FL
hsa-miR-488 FL
hsa-miR-505* FL
ebv-miR-BART16 FL
hsa-miR-891a FL
hsa-miR-221/mmu- FL
miR-221/rno-miR-221
hsa-miR-7/mmu-miR- FL
7a/rno-miR-7a
hsa-miR-299-3p FL
hsa-miR-575 FL
hsa-miR-585 FL
hsa-miR-30e*/mmu- FL
miR-30e*/rno-miR-
30e*
hcmv-miR-US25-1* FL
hsa-miR-708/mmu- FL
miR-708/rno-miR-708
hsv1-miR-H1 FL
hsa-let-7g/mmu-let-7g FL
hsa-miR-146a/mmu- FL
miR-146a/rno-miR-
146a
ebv-miR-BART8* FL
hsa-miR-106b*/mmu- FL
miR-106b*/rno-miR-
106b*
hsa-miR-601 FL
hsa-miR-553 FL
hsa-miR-518b FL
hsa-miR-548d-5p FL
hsa-miR-382/mmu- FL
miR-382/rno-miR-382
hsa-miR-630 FL
hsa-miR-144* FL
hsa-miR-519d FL
mghv-miR-M1-3 FL
hsa-miR-497/mmu- FL
miR-497/rno-miR-497
hsa-miR-524-5p FL
hsa-miR-500* FL
hsa-miR-920 FL
hsa-miR-297/mmu- FL
miR-297a
hsa-miR-509-3-5p FL
hsa-miR-340*/mmu- FL
miR-340-3p/rno-miR-
340-3p
hsa-miR-99b*/mmu- FL
miR-99b*/rno-miR-
99b*
hsa-miR-887 FL
hsa-miR-331-3p/mmu- FL
miR-331-3p/rno-miR-
331
hsa-miR-206/mmu- FL
miR-206/rno-miR-206
hsa-miR-377* FL
mghv-miR-M1-8 FL
hsa-miR-513a-3p FL
hsa-miR-146b-3p FL
hsa-miR-155* FL
hsa-miR-574-3p/mmu- FL
miR-574-3p
hsa-miR-615-3p/mmu- FL
miR-615-3p
hsa-miR-28-5p/mmu- FL
miR-28/rno-miR-28
hsa-miR-934 FL
hsa-miR-151-5p/mmu- FL
miR-151-5p/rno-miR-
151
hsa-miR-885-5p FL
hsa-miR-409-5p/mmu- FL
miR-409-5p/rno-miR-
409-5p
hsa-let-7d*/mmu-let- FL
7d*/rno-let-7d*
hsa-miR-155 FL
hsa-let-7b*/mmu-let- FL
7b*/rno-let-7b*
hsa-miR-7-2* FL
hsa-miR-221* FL
hsa-miR-9*/mmu-miR- FL
9*/rno-miR-9*
hsa-miR-122* FL
hsa-miR-130b/mmu- FL
miR-130b/rno-miR-
130b
hsa-miR-183/mmu- FL
miR-183/rno-miR-183
hsa-miR-92a/mmu- FL
miR-92a/rno-miR-92a
hsa-miR-890 FL
hsa-miR-938 FL
kshv-miR-K12-7 FL
hsa-miR-629* FL
hsa-miR-922 FL
kshv-miR-K12-5 FL
hsa-miR-197/mmu- FL
miR-197
hsa-miR-552 FL
hsa-miR-151-3p FL
hsa-miR-194/mmu- FL
miR-194/rno-miR-194
hsa-miR-218-2*/mmu- FL
miR-218-2*/rno-miR-
218*
hsa-miR-181b/mmu- FL
miR-181b/rno-miR-
181b
ebv-miR-BART18-3p FL
hsa-miR-34c-5p/mmu- FL
miR-34c/rno-miR-34c
hsa-miR-622 FL
hsa-miR-514 FL
hsa-miR-657 FL
hsa-miR-518a-3p FL
hsa-miR-647 FL
hsa-miR-22*/mmu- FL
miR-22*/rno-miR-22*
hsa-miR-196a*/mmu- FL
miR-196a*/rno-miR-
196a*
kshv-miR-K12-1 FL
hsa-miR-425/mmu- FL
miR-425/rno-miR-425
hsa-miR-361-3p FL
hsa-miR-220b FL
hsa-miR-744/mmu- FL
miR-744
hsa-miR-551a FL
hsa-miR-301a/mmu- FL
miR-301a/rno-miR-
301a
hsa-miR-92b/mmu- FL
miR-92b/rno-miR-92b
hsa-miR-487b/mmu- FL
miR-487b/rno-miR-
487b
hsa-miR-363*/rno-miR- FL
363*
hsa-miR-337-3p FL
hsa-miR-636 FL
hsa-miR-600 FL
hsa-miR-138/mmu- FL
miR-138/rno-miR-138
hsa-miR-96/mmu-miR- FL
96/rno-miR-96
hsa-miR-20b* FL
hsa-miR-342-5p/mmu- FL
miR-342-5p/rno-miR-
342-5p
hsa-miR-215 FL
hsa-miR-129* FL
TABLE 27
Predictor microRNAs that distinguish Burkitt lymphoma
from Hodgkin's lymphoma
Higher
BL vs HL in
hsa-miR-19b/mmu-miR- BL
19b/rno-miR-19b
hsa-miR-19a/mmu-miR- BL
19a/rno-miR-19a
hsa-miR-17/mmu-miR- BL
17/rno-miR-17-5p/rno-
miR-17
hsa-miR-106a BL
hsa-miR-20a/mmu-miR- BL
20a/rno-miR-20a
hsa-miR-106b/mmu-miR- BL
106b/rno-miR-106b
hsa-miR-30c/mmu-miR- BL
30c/rno-miR-30c
hsa-miR-551b/mmu-miR- HL
551b/rno-miR-551b
hsa-miR-921 HL
ebv-miR-BART2-3p HL
hsa-miR-32* HL
hsa-miR-494/mmu-miR- HL
494/rno-miR-494
hsa-miR-29c/mmu-miR- HL
29c/rno-miR-29c
hsa-miR-923 HL
hsa-miR-199b-5p HL
hsa-miR-148a/mmu-miR- HL
148a
hsa-miR-130a/mmu-miR- HL
130a/rno-miR-130a
hsa-miR-154/mmu-miR- HL
154/rno-miR-154
hsa-miR-151-5p/mmu- HL
miR-151-5p/rno-miR-151
hsa-miR-28-5p/mmu- HL
miR-28/rno-miR-28
hsa-miR-365/mmu-miR- HL
365/rno-miR-365
hsa-miR-602 HL
hsa-miR-222/mmu-miR- HL
222/rno-miR-222
hsa-miR-214/mmu-miR- HL
214/rno-miR-214
hsa-miR-144* HL
hsa-miR-107/mmu-miR- HL
107/rno-miR-107
hsa-miR-497/mmu-miR- HL
497/rno-miR-497
hsa-let-7g/mmu-let-7g HL
hsa-miR-146a/mmu-miR- HL
146a/rno-miR-146a
hsa-miR-186/mmu-miR- HL
186/rno-miR-186
hsa-miR-886-5p HL
hsa-miR-152/mmu-miR- HL
152/rno-miR-152
hsa-miR-29a*/mmu-miR- HL
29a*/rno-miR-29a*
hsa-miR-140-5p/mmu- HL
miR-140/rno-miR-140
hsa-miR-532-5p/mmu- HL
miR-532-5p/rno-miR-
532-5p
hsa-miR-145*/mmu-miR- HL
145*
hsa-miR-515-5p HL
hsa-miR-153/mmu-miR- HL
153/rno-miR-153
hsa-miR-513a-5p HL
hsa-miR-516a-5p HL
hsa-miR-660 HL
hsa-miR-29c*/mmu-miR- HL
29c*/rno-miR-29c*
hsa-miR-505/rno-miR- HL
505
hsa-miR-455-3p HL
hsa-miR-519e* HL
hsa-miR-502-3p HL
hsa-miR-922 HL
hsa-miR-524-5p HL
hsa-miR-483-5p HL
hsa-miR-708/mmu-miR- HL
708/rno-miR-708
hsa-miR-498 HL
ebv-miR-BART19-3p HL
hsa-miR-149/mmu-miR- HL
149
hsa-miR-574-3p/mmu- HL
miR-574-3p
hsa-miR-659 HL
hsa-miR-331-3p/mmu- HL
miR-331-3p/rno-miR-331
hsa-miR-105 HL
hsa-miR-128/mmu-miR- HL
128/rno-miR-128
hsa-miR-200b*/mmu- HL
miR-200b*
hsa-miR-381/mmu-miR- HL
381/rno-miR-381
hsa-miR-766 HL
hsa-miR-557 HL
ebv-miR-BART16 HL
hsa-miR-488 HL
hsa-miR-516b HL
mghv-miR-M1-2 HL
hsa-miR-891a HL
hsa-miR-221/mmu-miR- HL
221/rno-miR-221
hsa-miR-146b-3p HL
hsa-miR-526b HL
mghv-miR-M1-3 HL
hsa-miR-505* HL
hsv1-miR-H1 HL
hcmv-miR-UL70-3p HL
hsa-miR-24-2*/mmu- HL
miR-24-2*/rno-miR-24-
2*
hsa-miR-617 HL
hsa-miR-194/mmu-miR- HL
194/rno-miR-194
hsa-miR-934 HL
hsa-miR-220c HL
hsa-miR-548d-5p HL
hsa-miR-937 HL
ebv-miR-BART13 HL
hsa-miR-7/mmu-miR- HL
7a/rno-miR-7a
hsa-miR-210/mmu-miR- HL
210/rno-miR-210
hsa-miR-490-3p/mmu- HL
miR-490
hsa-miR-221* HL
hsa-miR-92a/mmu-miR- HL
92a/rno-miR-92a
hsa-miR-183*/mmu-miR- HL
183*
hsa-miR-513a-3p HL
hsa-miR-575 HL
hsa-miR-595 HL
hsa-miR-920 HL
hsa-miR-483-3p HL
hsa-miR-330-5p/mmu- HL
miR-330/rno-miR-330
hsa-miR-525-5p HL
hsa-miR-99b/mmu-miR- HL
99b/rno-miR-99b
hsa-miR-509-3p HL
hsa-miR-151-3p HL
ebv-miR-BHRF1-1 HL
hsa-miR-630 HL
mghv-miR-M1-7-3p HL
hsa-miR-328/mmu-miR- HL
328/rno-miR-328
hsa-miR-452 HL
hsa-miR-635 HL
ebv-miR-BART5 HL
hsa-miR-373* HL
hsa-miR-96/mmu-miR- HL
96/rno-miR-96
hsa-miR-382/mmu-miR- HL
382/rno-miR-382
hsa-miR-155 HL
hsa-miR-197/mmu-miR- HL
197
kshv-miR-K12-6-5p HL
hcmv-miR-UL112 HL
hsa-miR-551b* HL
hsa-miR-877/mmu-miR- HL
877/rno-miR-877
hsa-miR-589 HL
hsa-miR-936 HL
hsa-miR-34c-5p/mmu- HL
miR-34c/rno-miR-34c
hsa-miR-885-5p HL
ebv-miR-BART6-3p HL
hsa-miR-585 HL
hsa-miR-302c* HL
hsa-miR-196a*/mmu- HL
miR-196a*/rno-miR-
196a*
hsa-miR-195* HL
hsa-miR-17*/rno-miR-17- HL
3p
hsa-miR-296-5p/mmu- HL
miR-296-5p/rno-miR-
296*
hsa-miR-550* HL
ebv-miR-BHRF1-3 HL
hsa-miR-296-3p/mmu- HL
miR-296-3p/rno-miR-296
hsa-miR-526b* HL
hsa-miR-548b-3p HL
hsa-miR-652/mmu-miR- HL
652/rno-miR-652
hsa-miR-297/mmu-miR- HL
297a
hsa-miR-553 HL
hsa-miR-194* HL
hsa-miR-23a*/rno-miR- HL
23a*
hsa-miR-130b/mmu-miR- HL
130b/rno-miR-130b
hsa-miR-586 HL
hsa-miR-137/mmu-miR- HL
137/rno-miR-137
hsa-miR-610 HL
mghv-miR-M1-8 HL
hsa-miR-193b* HL
hsa-miR-519d HL
hsa-miR-125b-1*/mmu- HL
miR-125b-3p/rno-miR-
125b-3p
hsa-miR-744/mmu-miR- HL
744
hsa-miR-138/mmu-miR- HL
138/rno-miR-138
hsa-miR-21* HL
hsa-miR-576-5p HL
hsa-miR-125a-3p/mmu- HL
miR-125a-3p/rno-miR-
125a-3p
mghv-miR-M1-6 HL
hsa-miR-425/mmu-miR- HL
425/rno-miR-425
hsa-miR-10a*/mmu-miR- HL
10a*/rno-miR-10a-3p
hsa-miR-215 HL
hsa-miR-345 HL
hsa-miR-887 HL
hsa-miR-7-2* HL
hsa-miR-122* HL
hsa-miR-363*/rno-miR- HL
363*
hsa-miR-22*/mmu-miR- HL
22*/rno-miR-22*
hsa-miR-542-5p/mmu- HL
miR-542-5p/rno-miR-
542-5p
hsa-miR-92b* HL
hsa-miR-526a/hsa- HL
miR-520c-5p/hsa-
miR-518d-5p
kshv-miR-K12-5 HL
hsa-miR- HL
340*/mmu-miR-
340-3p/rno-miR-
340-3p
hsa-let-7d*/mmu- HL
let-7d*/rno-let-7d*
hsa-miR-92b/mmu- HL
miR-92b/rno-miR-
92b
hsa-miR-518b HL
hiv1-miR-H1 HL
hsa-miR-623 HL
hsa-miR-645 HL
hsa-miR-601 HL
hsa-miR-519e HL
hsa-miR-650 HL
hsa-miR-361-3p HL
hsa-miR- HL
150*/mmu-miR-
150*
hsa-miR- HL
425*/mmu-miR-
425*
hsa-miR- HL
135a*/mmu-miR-
135a*
hsa-miR-518a-3p HL
hsa-miR-612 HL
hsa-miR-212/mmu- HL
miR-212/rno-miR-
212
hsa-miR-125b- HL
2*/rno-miR-125b*
hsa-miR-500 HL
hsa-miR-663 HL
hsa-miR-647 HL
hsa-miR-502-5p HL
ebv-miR-BART18- HL
3p
hsa-miR- HL
99b*/mmu-miR-
99b*/rno-miR-99b*
ebv-miR-BART7* HL
hsa-miR-500* HL
hsa-miR- HL
124*/mmu-miR-
124*/rno-miR-124*
hsa-miR-206/mmu- HL
miR-206/rno-miR-
206
hsa-miR-615- HL
3p/mmu-miR-615-
3p
hsa-miR-30c- HL
1*/mmu-miR-30c-
1*/rno-miR-30c-1*
hsa-miR-331- HL
5p/mmu-miR-331-
5p
hcmv-miR-US25-1* HL
hsa-miR-326/mmu- HL
miR-326/rno-miR-
326
hsa-miR- HL
181b/mmu-miR-
181b/rno-miR-181b
hsa-miR-376a* HL
hsa-miR-433/mmu- HL
miR-433/rno-miR-
433
hsa-miR-640 HL
hsa-miR-938 HL
hsa-miR-508-5p HL
hsa-miR-484/mmu- HL
miR-484/rno-miR-
484
hsa-miR- HL
106b*/mmu-miR-
106b*/rno-miR-
106b*
hsa-miR-600 HL
hsa-let-7b*/mmu- HL
let-7b*/rno-let-7b*
hsa-miR-20b* HL
hsa-miR-622 HL
hsa-miR-657 HL
hsa-miR-183/mmu- HL
miR-183/rno-miR-
183
hsa-miR-409- HL
5p/mmu-miR-409-
5p/rno-miR-409-5p
ebv-miR-BART20- HL
3p
ebv-miR-BART8* HL
hsa-miR-129* HL
hsa-miR-629 HL
hsa-miR-890 HL
hsa-miR-208a/mmu- HL
miR-208a/rno-miR-
208
hsa-miR-492 HL
hsa-miR-629* HL
hsa-miR-654-5p HL
hsa-miR-584 HL
hsa-miR-636 HL
hsa-miR-490-5p HL
hsa-miR-337-3p HL
hsa-miR-9*/mmu- HL
miR-9*/rno-miR-9*
ebv-miR-BART9* HL
hsa-miR-509-3-5p HL
hsa-miR-342- HL
5p/mmu-miR-342-
5p/rno-miR-342-5p
hsa-miR-514 HL
hsa-miR-377* HL
kshv-miR-K12-1 HL
hsa-miR-124/mmu- HL
miR-124/rno-miR-
124
hsa-miR-542- HL
3p/mmu-miR-542-
3p/rno-miR-542-3p
hsa-miR-220b HL
hsa-miR-299-3p HL
hsa-miR-181a-2* HL
hsa-miR-202 HL
hsa-miR- HL
487b/mmu-miR-
487b/rno-miR-487b
hsa-miR-551a HL
hsa-miR-338- HL
5p/mmu-miR-338-
5p/rno-miR-338*
hsa-miR-552 HL
TABLE 28
Predictor microRNAs that distinguish chronic lymphocytic leukemia
from follicular lymphoma
Higher
CLL vs FL in
hsa-miR-331-5p/mmu- CLL
miR-331-5p
hsa-miR-144/mmu-miR- CLL
144/rno-miR-144
hsa-miR-150/mmu-miR- CLL
150/rno-miR-150
hsa-miR-140-5p/mmu- CLL
miR-140/rno-miR-140
hsa-miR-335/mmu-miR- CLL
335-5p/rno-miR-335
hsa-miR-186/mmu-miR- CLL
186/rno-miR-186
hsa-miR-486-5p/mmu- CLL
miR-486
hsa-miR-154/mmu-miR- CLL
154/rno-miR-154
hsa-miR-223/mmu-miR- CLL
223/rno-miR-223
hsa-miR-299-5p/mmu- CLL
miR-299*/rno-miR-299
hsa-let-7g/mmu-let-7g CLL
hsa-miR-32/mmu-miR- CLL
32/rno-miR-32
hsa-miR-30e*/mmu-miR- CLL
30e*/rno-miR-30e*
hsa-miR-147 CLL
hsa-miR-20b/mmu-miR- CLL
20b/rno-miR-20b-5p
hsa-miR-101/mmu-miR- CLL
101a/rno-miR-101a
hsa-let-7f/mmu-let-7f/rno- CLL
let-7f
hsa-miR-30e/mmu-miR- CLL
30e/rno-miR-30e
hsa-miR-668/mmu-miR- CLL
668
hsa-miR-768-5p CLL
hsa-miR-19a/mmu-miR- CLL
19a/rno-miR-19a
hsa-miR-199a-5p/mmu- CLL
miR-199a-5p/rno-miR-
199a-5p
hsa-miR-638 CLL
hsa-miR-196a*/mmu-miR- CLL
196a*/rno-miR-196a*
hsa-miR-19b/mmu-miR- CLL
19b/rno-miR-19b
hsa-miR-30d/mmu-miR- CLL
30d/rno-miR-30d
hsa-miR-363/mmu-miR- CLL
363/rno-miR-363
hsa-miR-374a CLL
hsa-miR-140-3p/mmu- CLL
miR-140*/rno-miR-140*
hsa-miR-185/mmu-miR- CLL
185/rno-miR-185
hsa-miR-106b/mmu-miR- CLL
106b/rno-miR-106b
hsa-miR-106a CLL
hsa-miR-191/mmu-miR- CLL
191/rno-miR-191
hsa-miR-17/mmu-miR- CLL
17/rno-miR-17-5p/rno-
miR-17
hsa-let-7i/mmu-let-7i/rno- CLL
let-7i
hsa-miR-20a/mmu-miR- CLL
20a/rno-miR-20a
hsa-miR-142-5p/mmu- CLL
miR-142-5p/rno-miR-142-
5p
hsa-miR-768-3p CLL
hsa-miR-30b/mmu-miR- CLL
30b/rno-miR-30b-5p
hsa-miR-891a CLL
hsa-miR-24-1*/mmu-miR- CLL
24-1*/rno-miR-24-1*
hsa-miR-29c/mmu-miR- CLL
29c/rno-miR-29c
hsa-miR-28-5p/mmu-miR- CLL
28/rno-miR-28
hsa-miR-30a/mmu-miR- CLL
30a/rno-miR-30a
hsa-miR-155 CLL
hsa-miR-361-5p/mmu- CLL
miR-361/rno-miR-361
hsa-miR-15a/mmu-miR- CLL
15a
hsa-miR-26a/mmu-miR- CLL
26a/rno-miR-26a
hsa-miR-30c/mmu-miR- CLL
30c/rno-miR-30c
hsa-miR-541* CLL
hsa-miR-26b/mmu-miR- CLL
26b/rno-miR-26b
hsa-miR-519d CLL
hsa-miR-15b/mmu-miR- CLL
15b/rno-miR-15b
hsa-miR-550 CLL
hsa-miR-29b/mmu-miR- CLL
29b/rno-miR-29b
hsa-miR-29a/mmu-miR- CLL
29a/rno-miR-29a
hsa-miR-103/mmu-miR- CLL
103/rno-miR-103
hsa-miR-423-3p/mmu- CLL
miR-423-3p/rno-miR-423
hsa-miR-549 CLL
hsa-miR-107/mmu-miR- CLL
107/rno-miR-107
hsa-miR-888* CLL
hsa-miR-801/mmu-miR- CLL
801
hsa-miR-149* FL
hsa-miR-634 FL
ebv-miR-BART2-3p FL
hsa-miR-921 FL
hsa-miR-494/mmu-miR- FL
494/rno-miR-494
hsa-miR-933 FL
mghv-miR-M1-4 FL
hsa-let-7e/mmu-let-7e/rno- FL
let-7e
hsa-miR-939 FL
hsa-miR-518c* FL
hsa-miR-32* FL
hsa-miR-491-3p FL
hsa-miR-185/mmu-miR- FL
185/rno-miR-185
hsa-miR-765 FL
ebv-miR-BART17-5p FL
hsa-miR-576-3p FL
hsa-miR-658 FL
hsa-miR-503 FL
hsa-miR-30b* FL
hsa-miR-302a/mmu-miR- FL
302a
hsa-miR-628-3p FL
hsa-miR-642 FL
hsa-miR-620 FL
hsa-miR-99a/mmu- FL
miR-99a/rno-miR-
99a
hsa-miR-371-5p FL
hsa-miR-452 FL
hsa-miR-126*/mmu- FL
miR-126-5p/rno-
miR-126*
hsa-miR-298 FL
hsa-miR-193a-5p FL
hsa-miR-583 FL
hsa-miR-143/mmu- FL
miR-143/rno-miR-
143
hsa-miR-665 FL
hsa-miR-505/rno- FL
miR-505
hsa-miR-199b-5p FL
hsa-miR-28-3p/rno- FL
miR-28*
hsa-miR-422a FL
hsa-miR-515-5p FL
hsa-miR-455-3p FL
hsa-miR-10a/mmu- FL
miR-10a/rno-miR-
10a-5p
hsa-miR-300 FL
ebv-miR-BART5 FL
hsa-miR-10b/mmu- FL
miR-10b/rno-miR-
10b
hsa-miR-212/mmu- FL
miR-212/rno-miR-
212
hsa-miR-145/mmu- FL
miR-145/rno-miR-
145
hsa-miR-187* FL
ebv-miR-BHRF1-1 FL
ebv-miR-BHRF1-2 FL
hsa-miR-126/mmu- FL
miR-126-3p/rno-
miR-126
hsa-miR- FL
130b*/mmu-miR-
130b*
hsa-miR-326/mmu- FL
miR-326/rno-miR-
326
mghv-miR-M1-2 FL
kshv-miR-K12-6-3p FL
hsa-miR-516b FL
hsa-miR-519e* FL
mghv-miR-M1-7-3p FL
hsa-miR-629 FL
hsa-miR-24-2*/mmu- FL
miR-24-2*/rno-miR-
24-2*
hsa-miR-943 FL
hsa-miR-124/mmu- FL
miR-124/rno-miR-
124
hsa-miR-365/mmu- FL
miR-365/rno-miR-
365
hsa-miR-493 FL
hsa-miR-29c*/mmu- FL
miR-29c*/rno-miR-
29c*
hsa-miR-602 FL
hsa-miR-484/mmu- FL
miR-484/rno-miR-
484
hsa-miR-483-3p FL
hsa-miR-125b- FL
2*/rno-miR-125b*
hsa-miR-675 FL
mghv-miR-M1-7-5p FL
hsa-miR-152/mmu- FL
miR-152/rno-miR-
152
hsa-miR-27a*/mmu- FL
miR-27a*/rno-miR-
27a*
hsa-miR-542- FL
3p/mmu-miR-542-
3p/rno-miR-542-3p
hsa-miR-100/mmu- FL
miR-100/rno-miR-
100
hsa-miR-208a/mmu- FL
miR-208a/rno-miR-
208
hsa-miR-766 FL
hsa-miR-637 FL
hsa-miR-519c- FL
5p/hsa-miR-519b-
5p/hsa-miR-
523*/hsa-miR-
518e*/hsa-miR-
522*/hsa-miR-519a*
hsa-miR-409- FL
5p/mmu-miR-409-
5p/rno-miR-409-5p
hsa-miR-199a- FL
3p/hsa-miR-199b-
3p/mmu-miR-199a-
3p/mmu-miR-
199b/rno-miR-199a-
3p
hsa-miR- FL
106b*/mmu-miR-
106b*/rno-miR-
106b*
hsa-miR-130a/mmu- FL
miR-130a/rno-miR-
130a
hsa-miR-645 FL
hsa-miR-548d-5p FL
hsa-miR-671- FL
5p/mmu-miR-671-5p
hsa-miR-574- FL
3p/mmu-miR-574-3p
hsa-miR-125b/mmu- FL
miR-125b-5p/rno-
miR-125b-5p
hsa-miR-500* FL
hsa-miR-425/mmu- FL
miR-425/rno-miR-
425
ebv-miR-BART8* FL
hsa-miR-377* FL
hsa-miR-513a-3p FL
hsa-miR-181a-2* FL
hsa-miR-938 FL
hsa-miR-155* FL
hsa-miR-218- FL
2*/mmu-miR-218-
2*/rno-miR-218*
hsa-miR-198 FL
hsa-miR-151-3p FL
hsa-miR-299-3p FL
hcmv-miR-US25-1* FL
hsa-miR-708/mmu- FL
miR-708/rno-miR-
708
hsa-miR-659 FL
hsa-miR-600 FL
hsa-miR-601 FL
hsa-miR-296- FL
3p/mmu-miR-296-
3p/rno-miR-296
hsa-let-7b*/mmu-let- FL
7b*/rno-let-7b*
ebv-miR-BART6-3p FL
hsa-miR-7-2* FL
hsa-miR-9*/mmu- FL
miR-9*/rno-miR-9*
hsa-miR-509-3-5p FL
hsa-miR-518b FL
hsa-miR-183*/mmu- FL
miR-183*
hsa-miR-125b- FL
1*/mmu-miR-125b-
3p/rno-miR-125b-3p
hsa-miR-183/mmu- FL
miR-183/rno-miR-
183
hsa-miR-890 FL
hsa-miR-153/mmu- FL
miR-153/rno-miR-
153
hsa-miR-874/mmu- FL
miR-874/rno-miR-
874
hsa-miR-220c FL
hsa-miR-99b*/mmu- FL
miR-99b*/rno-miR-
99b*
hsa-miR-193b* FL
hsa-miR-629* FL
hcmv-miR-UL148D FL
ebv-miR-BART7* FL
hsa-miR-99b/mmu- FL
miR-99b/rno-miR-
99b
hsa-miR-206/mmu- FL
miR-206/rno-miR-
206
hsa-miR-381/mmu- FL
miR-381/rno-miR-
381
hsa-miR-194/mmu- FL
miR-194/rno-miR-
194
hsa-miR-525-5p FL
hsa-miR-193b FL
hsa-miR-497/mmu- FL
miR-497/rno-miR-
497
ebv-miR-BART18- FL
3p
hsa-miR-424 FL
hsa-miR-553 FL
hsa-let-7d*/mmu-let- FL
7d*/rno-let-7d*
hsa-miR-34c- FL
5p/mmu-miR-
34c/rno-miR-34c
ebv-miR-BHRF1-3 FL
kshv-miR-K12-6-5p FL
hsa-miR-551a FL
hsa-miR-195* FL
hsa-miR-551b* FL
hsa-miR-514 FL
hsa-miR-552 FL
hsa-miR-122* FL
hsa-miR-92b/mmu- FL
miR-92b/rno-miR-
92b
hsa-miR-22*/mmu- FL
miR-22*/rno-miR-
22*
hsa-miR-635 FL
kshv-miR-K12-1 FL
hsa-miR-483-5p FL
hsa-miR-340*/mmu- FL
miR-340-3p/rno-
miR-340-3p
hsa-miR-615- FL
3p/mmu-miR-615-3p
hsa-miR-505* FL
hsa-miR-622 FL
hsa-miR-181b/mmu- FL
miR-181b/rno-miR-
181b
hsa-miR-886-5p FL
hsa-miR-885-5p FL
hsa-miR-220b FL
hsa-miR-524-5p FL
hsa-miR-382/mmu- FL
miR-382/rno-miR-
382
hsa-miR-744/mmu- FL
miR-744
hsv1-miR-H1 FL
hsa-miR-526b FL
hsa-miR-657 FL
hsa-miR-130b/mmu- FL
miR-130b/rno-miR-
130b
hsa-miR-181a/mmu- FL
miR-181a/rno-miR-
181a
hsa-miR-301a/mmu- FL
miR-301a/rno-miR-
301a
hsa-miR-490- FL
3p/mmu-miR-490
hsa-miR-485- FL
3p/mmu-miR-485*
hsa-miR-297/mmu- FL
miR-297a
hsa-miR-630 FL
hsa-miR-877/mmu- FL
miR-877/rno-miR-
877
kshv-miR-K12-5 FL
hsa-miR-617 FL
mghv-miR-M1-3 FL
hsa-miR-920 FL
hsa-miR-585 FL
hsa-miR-374b* FL
hsa-miR-215 FL
hsa-miR-342- FL
5p/mmu-miR-342-
5p/rno-miR-342-5p
hsa-miR-934 FL
hsa-miR-575 FL
hsa-miR-488 FL
ebv-miR-BART16 FL
hsa-miR-647 FL
hsa-miR-138/mmu- FL
miR-138/rno-miR-
138
hsa-miR-221* FL
hsa-miR- FL
200b*/mmu-miR-
200b*
hsa-miR-337-3p FL
hsa-miR-922 FL
hsa-miR-197/mmu- FL
miR-197
hsa-miR-96/mmu- FL
miR-96/rno-miR-96
hsa-miR-518a-3p FL
TABLE 29
Predictor microRNAs that distinguish Burkitt lymphoma
from Hodgkin's lymphoma
Higher
CLL vs HL in
hsa-miR-32/mmu-miR- CLL
32/rno-miR-32
hsa-miR-30e*/mmu-miR- CLL
30e*/rno-miR-30e*
hsa-let-7g/mmu-let-7g CLL
hsa-miR-144/mmu-miR- CLL
144/rno-miR-144
hsa-miR-140-5p/mmu- CLL
miR-140/rno-miR-140
hsa-miR-19a/mmu-miR- CLL
19a/rno-miR-19a
hsa-miR-154/mmu-miR- CLL
154/rno-miR-154
hsa-miR-150/mmu-miR- CLL
150/rno-miR-150
hsa-miR-28-5p/mmu- CLL
miR-28/rno-miR-28
hsa-miR-363/mmu-miR- CLL
363/rno-miR-363
hsa-miR-101/mmu-miR- CLL
101a/rno-miR-101a
hsa-miR-299-5p/mmu- CLL
miR-299*/rno-miR-299
hsa-miR-768-5p CLL
hsa-miR-19b/mmu-miR- CLL
19b/rno-miR-19b
hsa-miR-30e/mmu-miR- CLL
30e/rno-miR-30e
hsa-miR-20b/mmu-miR- CLL
20b/rno-miR-20b-5p
hsa-miR-374a CLL
hsa-let-7f/mmu-let-7f/rno- CLL
let-7f
hsa-miR-335/mmu-miR- CLL
335-5p/rno-miR-335
hsa-miR-142-5p/mmu- CLL
miR-142-5p/rno-miR-142-
5p
hsa-miR-486-5p/mmu- CLL
miR-486
hsa-miR-33a/mmu-miR- CLL
33/rno-miR-33
hsa-miR-30b/mmu-miR- CLL
30b/rno-miR-30b-5p
hsa-miR-768-3p CLL
hsa-miR-668/mmu-miR- CLL
668
hsa-miR-15b/mmu-miR- CLL
15b/rno-miR-15b
hsa-miR-196a*/mmu- CLL
miR-196a*/rno-miR-196a*
hsa-miR-140-3p/mmu- CLL
miR-140*/rno-miR-140*
hsa-miR-29b/mmu-miR- CLL
29b/rno-miR-29b
hsa-miR-29c/mmu-miR- CLL
29c/rno-miR-29c
hsa-miR-186/mmu-miR- CLL
186/rno-miR-186
hsa-miR-106a CLL
hsa-miR-26a/mmu-miR- CLL
26a/rno-miR-26a
hsa-miR-106b/mmu-miR- CLL
106b/rno-miR-106b
hsa-miR-17/mmu-miR- CLL
17/rno-miR-17-5p/rno-
miR-17
hsa-miR-191/mmu-miR- CLL
191/rno-miR-191
hsa-miR-20a/mmu-miR- CLL
20a/rno-miR-20a
hsa-miR-30c/mmu-miR- CLL
30c/rno-miR-30c
hsa-miR-26b/mmu-miR- CLL
26b/rno-miR-26b
hsa-miR-147 CLL
hsa-miR-15a/mmu-miR- CLL
15a
hsa-miR-30d/mmu-miR- CLL
30d/rno-miR-30d
hsa-miR-199a-5p/mmu- CLL
miR-199a-5p/rno-miR-
199a-5p
hsa-miR-29a/mmu-miR- CLL
29a/rno-miR-29a
hsa-miR-223/mmu-miR- CLL
223/rno-miR-223
hsa-miR-30a/mmu-miR- CLL
30a/rno-miR-30a
hsa-miR-16/mmu-miR- CLL
16/rno-miR-16
hsa-miR-451/mmu-miR- CLL
451/rno-miR-451
hsa-miR-24-1*/mmu-miR- CLL
24-1*/rno-miR-24-1*
hsa-miR-550 CLL
hsa-miR-342-3p/mmu- CLL
miR-342-3p/rno-miR-342-
3p
hsa-miR-195/mmu-miR- CLL
195/rno-miR-195
hsa-miR-801/mmu-miR- CLL
801
hsa-miR-541* CLL
hsa-let-7i/mmu-let-7i/rno- CLL
let-7i
hsa-miR-155 CLL
hsa-miR-185/mmu-miR- CLL
185/rno-miR-185
hsa-miR-891a CLL
hsa-miR-138-1*/mmu- CLL
miR-138*/rno-miR-138*
hsa-miR-27b/mmu-miR- CLL
27b/rno-miR-27b
hsa-miR-361-5p/mmu- CLL
miR-361/rno-miR-361
hsa-miR-129* CLL
hsa-miR-638 CLL
hsa-miR-34b/mmu-miR- CLL
34b-3p
hsa-miR-107/mmu-miR- CLL
107/rno-miR-107
hsa-miR-549 CLL
hsa-miR-888* CLL
hsa-miR-423-3p/mmu- CLL
miR-423-3p/rno-miR-423
hsa-let-7e/mmu-let- HL
7e/rno-let-7e
hsa-miR-125a-5p/mmu- HL
miR-125a-5p/rno-miR-
125a-5p
hsa-miR-576-3p HL
hsa-miR-513a-5p HL
ebv-miR-BART17-5p HL
hsa-miR-185/mmu-miR- HL
185/rno-miR-185
hsa-miR-921 HL
hsa-miR-518c* HL
hsa-miR-520d-5p HL
hsa-miR-939 HL
hsa-miR-634 HL
hsa-miR-491-3p HL
ebv-miR-BART2-3p HL
hsa-miR-30c-2*/mmu- HL
miR-30c-2*/rno-miR-30c-
2*
hsa-miR-765 HL
hsa-miR-923 HL
hsa-miR-620 HL
hsa-miR-933 HL
hsa-miR-143/mmu-miR- HL
143/rno-miR-143
hsa-miR-494/mmu-miR- HL
494/rno-miR-494
hsa-miR-665 HL
hsa-miR-642 HL
hsa-miR-126*/mmu-miR- HL
126-5p/rno-miR-126*
hsa-miR-658 HL
hsa-miR-149* HL
hsa-miR-30b* HL
mghv-miR-M1-4 HL
hsa-miR-99a/mmu-miR- HL
99a/rno-miR-99a
hsa-miR-193a-5p HL
hsa-miR-498 HL
hsa-miR-628-3p HL
hsa-miR-185* HL
hsa-miR-371-5p HL
hsa-miR-199b-5p HL
hsa-miR-126/mmu-miR- HL
126-3p/rno-miR-126
hsa-miR-503 HL
hsa-miR-10a/mmu-miR- HL
10a/rno-miR-10a-5p
hsa-miR-300 HL
hsa-miR-583 HL
hsa-miR-518a-5p/hsa- HL
miR-527
hsa-miR-10b/mmu-miR- HL
10b/rno-miR-10b
hsa-miR-145/mmu- HL
miR-145/rno-miR-
145
hsa-miR-128/mmu- HL
miR-128/rno-miR-
128
hsa-miR-532- HL
5p/mmu-miR-532-
5p/rno-miR-532-5p
hsa-miR-143* HL
hsa-miR-28-3p/rno- HL
miR-28*
hsa-miR- HL
130b*/mmu-miR-
130b*
hsa-miR-505/rno- HL
miR-505
hsa-miR-25* HL
hsa-miR-574- HL
3p/mmu-miR-574-3p
hsa-miR-455-3p HL
kshv-miR-K12-3 HL
hsa-miR-516b HL
kshv-miR-K12-8 HL
hsa-miR-502-3p HL
kshv-miR-K12-6-3p HL
hsa-miR-129- HL
5p/mmu-miR-129-
5p/rno-miR-129
hsa-miR-515-5p HL
hsa-miR-199a- HL
3p/hsa-miR-199b-
3p/mmu-miR-199a-
3p/mmu-miR-
199b/rno-miR-199a-
3p
hsa-miR-149/mmu- HL
miR-149
hsa-miR-889 HL
hsa-miR-637 HL
hsa-miR-600 HL
hsa-miR-151-3p HL
hsa-miR-656 HL
hsa-miR-497/mmu- HL
miR-497/rno-miR-
497
hsa-miR-152/mmu- HL
miR-152/rno-miR-
152
hsa-miR-100/mmu- HL
miR-100/rno-miR-
100
hsa-miR-425/mmu- HL
miR-425/rno-miR-
425
hsa-miR-145*/mmu- HL
miR-145*
hsa-miR-365/mmu- HL
miR-365/rno-miR-
365
hsa-miR-422a HL
hcmv-miR-UL70-3p HL
hsa-miR-27a*/mmu- HL
miR-27a*/rno-miR-
27a*
hsa-miR-194/mmu- HL
miR-194/rno-miR-
194
hsa-miR-548d-5p HL
hsa-miR-187* HL
hsa-miR-323- HL
3p/mmu-miR-323-
3p/rno-miR-323
hsa-miR-708/mmu- HL
miR-708/rno-miR-
708
hsa-miR-29c*/mmu- HL
miR-29c*/rno-miR-
29c*
hsa-miR-513a-3p HL
hsa-miR-595 HL
hsa-miR-483-3p HL
hsa-miR-330- HL
5p/mmu-miR-
330/rno-miR-330
hsa-miR-519e* HL
hsa-miR-509-3p HL
hsa-miR-328/mmu- HL
miR-328/rno-miR-
328
hsa-miR-373* HL
hsa-miR-96/mmu- HL
miR-96/rno-miR-96
hsa-miR-215 HL
hsa-miR-589 HL
hsa-miR-34c- HL
5p/mmu-miR-
34c/rno-miR-34c
hsa-miR-125b/mmu- HL
miR-125b-5p/rno-
miR-125b-5p
hsa-miR-130a/mmu- HL
miR-130a/rno-miR-
130a
hsa-miR-519c- HL
5p/hsa-miR-519b-
5p/hsa-miR-
523*/hsa-miR-
518e*/hsa-miR-
522*/hsa-miR-519a*
mghv-miR-M1-7-5p HL
hsa-miR-516a-5p HL
hsa-miR-424 HL
hsa-miR-17*/rno- HL
miR-17-3p
hsa-miR-296- HL
5p/mmu-miR-296-
5p/rno-miR-296*
hsa-miR-550* HL
hsa-miR-210/mmu- HL
miR-210/rno-miR-
210
hsa-miR-92b/mmu- HL
miR-92b/rno-miR-
92b
hsa-miR-548b-3p HL
hsa-miR-652/mmu- HL
miR-652/rno-miR-
652
hsa-miR-138/mmu- HL
miR-138/rno-miR-
138
hsa-miR-194* HL
hsa-miR-23a*/rno- HL
miR-23a*
hsa-miR-153/mmu- HL
miR-153/rno-miR-
153
hsa-miR-586 HL
hsa-miR-137/mmu- HL
miR-137/rno-miR-
137
hsa-miR-610 HL
hsa-miR-381/mmu- HL
miR-381/rno-miR-
381
hsa-miR-936 HL
hsa-miR-744/mmu- HL
miR-744
ebv-miR-BART5 HL
ebv-miR-BHRF1-1 HL
hsa-miR-21* HL
hsa-miR-576-5p HL
mghv-miR-M1-6 HL
hsa-miR-193b HL
hsa-miR-10a*/mmu- HL
miR-10a*/rno-miR-
10a-3p
hsa-miR-524-5p HL
hsa-miR-452 HL
hsa-miR-345 HL
hsa-miR-7-2* HL
hsa-miR-409- HL
5p/mmu-miR-409-
5p/rno-miR-409-5p
hsa-miR-557 HL
hsa-miR-181a/mmu- HL
miR-181a/rno-miR-
181a
hsa-miR-22*/mmu- HL
miR-22*/rno-miR-22*
hsa-miR-922 HL
hsa-miR-92b* HL
hsa-miR-938 HL
hsa-miR-526a/hsa- HL
miR-520c-5p/hsa-
miR-518d-5p
hsa-miR-526b* HL
ebv-miR-BHRF1-2 HL
hiv1-miR-H1 HL
hsa-miR-623 HL
mghv-miR-M1-2 HL
mghv-miR-M1-7-3p HL
hsa-miR-519e HL
hsa-miR-650 HL
hsa-miR-766 HL
hsa-miR-602 HL
hsa-miR-425*/mmu- HL
miR-425*
hsa-miR- HL
135a*/mmu-miR-
135a* HL
hsa-miR-612 HL
hsa-miR-212/mmu- HL
miR-212/rno-miR-
212
hsa-miR-125b- HL
2*/rno-miR-125b*
hcmv-miR-UL112 HL
hsa-miR-374b*
hsa-miR-886-5p HL
hsa-miR-500 HL
hsa-miR-502-5p HL
ebv-miR-BART18- HL
3p
hsa-miR-198 HL
hsa-miR-500* HL
hsa-miR-342- HL
5p/mmu-miR-342-
5p/rno-miR-342-5p
hsa-miR-124*/mmu- HL
miR-124*/rno-miR-
124*
hsa-miR-30c- HL
1*/mmu-miR-30c-
1*/rno-miR-30c-1*
hsa-miR-220c HL
hsa-miR-376a* HL
hsa-miR-640 HL
hcmv-miR-UL148D HL
hsa-miR-659 HL
hsa-miR-934 HL
hsa-miR-125a- HL
3p/mmu-miR-125a-
3p/rno-miR-125a-3p
hsa-miR-885-5p HL
hsa-miR-24- HL
2*/mmu-miR-24-
2*/rno-miR-24-2*
hsa-miR-484/mmu- HL
miR-484/rno-miR-
484
hsa-miR- HL
106b*/mmu-miR-
106b*/rno-miR-
106b*
hsa-miR-505* HL
hsa-let-7b*/mmu-let- HL
7b*/rno-let-7b*
hsa-miR-302c* HL
hsa-miR-542- HL
5p/mmu-miR-542-
5p/rno-miR-542-5p
hsv1-miR-H1 HL
mghv-miR-M1-3 HL
hsa-miR-183/mmu- HL
miR-183/rno-miR-
183
hsa-miR-122* HL
hsa-miR-183*/mmu- HL
miR-183*
hsa-miR-675 HL
hsa-miR-99b/mmu- HL
miR-99b/rno-miR-
99b
hsa-miR-874/mmu- HL
miR-874/rno-miR-
874
ebv-miR-BART20- HL
3p
hsa-miR-483-5p HL
hsa-miR-671- HL
5p/mmu-miR-671-5p
hsa-miR-629 HL
hsa-miR-553 HL
hsa-let-7d*/mmu-let- HL
7d*/rno-let-7d*
hsa-miR-601 HL
hsa-miR-645 HL
hsa-miR-920 HL
hsa-miR-525-5p HL
hsa-miR-221* HL
hsa-miR-890 HL
hsa-miR-492 HL
hsa-miR-629* HL
hsa-miR-635 HL
hsa-miR-130b/mmu- HL
miR-130b/rno-miR-
130b
hsa-miR-197/mmu- HL
miR-197
hsa-miR-654-5p HL
hsa-miR-518b HL
hsa-miR-382/mmu- HL
miR-382/rno-miR-
382
hsa-miR-584 HL
hsa-miR-99b*/mmu- HL
miR-99b*/rno-miR-
99b*
hsa-miR-630 HL
hsa-miR-490-5p HL
hsa-miR-663 HL
hsa-miR-337-3p HL
hsa-miR-9*/mmu- HL
miR-9*/rno-miR-9*
hsa-miR-202 HL
ebv-miR-BART16 HL
ebv-miR-BART9* HL
hsa-miR-193b* HL
ebv-miR-BART8* HL
hsa-miR-206/mmu- HL
miR-206/rno-miR-
206
hcmv-miR-US25-1* HL
hsa-miR-514 HL
kshv-miR-K12-6-5p HL
hsa-miR-488 HL
hsa-miR-508-5p HL
hsa-miR-551b* HL
hsa-miR-377* HL
ebv-miR-BART6-3p HL
hsa-miR-181b/mmu- HL
miR-181b/rno-miR-
181b
hsa-miR-526b HL
hsa-miR-622 HL
kshv-miR-K12-1 HL
hsa-miR-485- HL
3p/mmu-miR-485*
hsa-miR-490- HL
3p/mmu-miR-490
hsa-miR-125b- HL
1*/mmu-miR-125b-
3p/rno-miR-125b-3p
hsa-miR-124/mmu- HL
miR-124/rno-miR-
124
hsa-miR-657 HL
ebv-miR-BHRF1-3 HL
hsa-miR-542- HL
3p/mmu-miR-542-
3p/rno-miR-542-3p
kshv-miR-K12-5 HL
hsa-miR-943 HL
hsa-miR-551a HL
hsa-miR-297/mmu- HL
miR-297a
hsa-miR-296- HL
3p/mmu-miR-296-
3p/rno-miR-296
hsa-miR-617 HL
hsa-miR-195* HL
hsa-miR-575 HL
hsa-miR-208a/mmu- HL
miR-208a/rno-miR-
208
hsa-miR-647 HL
hsa-miR-509-3-5p HL
hsa-miR-340*/mmu- HL
miR-340-3p/rno-
miR-340-3p
hsa-miR-220b HL
hsa-miR- HL
200b*/mmu-miR-
200b*
hsa-miR-585 HL
hsa-miR-877/mmu- HL
miR-877/rno-miR-
877
hsa-miR-326/mmu- HL
miR-326/rno-miR-
326
ebv-miR-BART7* HL
hsa-miR-615- HL
3p/mmu-miR-615-3p
hsa-miR-433/mmu- HL
miR-433/rno-miR-
433
hsa-miR-338- HL
5p/mmu-miR-338-
5p/rno-miR-338*
hsa-miR-299-3p HL
hsa-miR-518a-3p HL
hsa-miR-181a-2* HL
hsa-miR-552 HL
TABLE 30
Predictor microRNAs that distinguish follicular lymphoma
from Hodgkin's lymphoma
Higher
FL vs HL in
hsa-miR-301a/mmu-miR- FL
301a/rno-miR-301a
kshv-miR-K12-7 FL
hsa-miR-96/mmu-miR- FL
96/rno-miR-96
hsa-miR-151-5p/mmu- FL
miR-151-5p/rno-miR-151
hsa-miR-28-5p/mmu-miR- FL
28/rno-miR-28
hsa-miR-302a/mmu-miR- FL
302a
hsa-miR-215 FL
hsa-miR-15b/mmu-miR- FL
15b/rno-miR-15b
hsa-miR-29b/mmu-miR- FL
29b/rno-miR-29b
hsa-miR-138/mmu-miR- FL
138/rno-miR-138
hsa-miR-363/mmu-miR- FL
363/rno-miR-363
hsa-miR-142-5p/mmu- FL
miR-142-5p/rno-miR-142-
5p
hsa-miR-19a/mmu-miR- FL
19a/rno-miR-19a
hsa-miR-497/mmu-miR- FL
497/rno-miR-497
hsa-miR-144* FL
hsa-miR-16/mmu-miR- FL
16/rno-miR-16
hsa-miR-138-1*/mmu- FL
miR-138*/rno-miR-138*
hsa-miR-768-5p FL
hsa-miR-30c/mmu-miR- FL
30c/rno-miR-30c
hsa-miR-129* FL
hsa-miR-801/mmu-miR- FL
801
hsa-miR-34b/mmu-miR- FL
34b-3p
hsa-miR-363*/rno-miR- FL
363*
hsa-miR-20b* FL
hsa-miR-550 FL
hsa-miR-600 FL
hsa-miR-196a*/mmu- FL
miR-196a*/rno-miR-196a*
hsa-miR-574-3p/mmu- FL
miR-574-3p
hsa-miR-620 FL
hsa-miR-331-3p/mmu- FL
miR-331-3p/rno-miR-331
hsa-let-7e/mmu-let- FL
7e/rno-let-7e
hsa-miR-524-5p FL
hsa-miR-197/mmu-miR- FL
197
hsa-miR-24-1*/mmu-miR- FL
24-1*/rno-miR-24-1*
hsa-miR-519e* HL
hsa-miR-628-3p HL
mghv-miR-M1-7-5p HL
hsa-miR-498 HL
hsa-miR-525-5p HL
hsa-miR-520d-5p HL
hsa-miR-551b* HL
hsa-miR-340*/mmu- HL
miR-340-3p/rno-
miR-340-3p
hsa-miR-889 HL
hsa-miR-494/mmu- HL
miR-494/rno-miR-
494
hsa-miR-874/mmu- HL
miR-874/rno-miR-
874
hsa-miR-30c- HL
2*/mmu-miR-30c-
2*/rno-miR-30c-2*
hsa-miR-183*/mmu- HL
miR-183*
hsa-miR-25* HL
hsa-miR-513a-5p HL
hsa-miR-198 HL
hsa-miR-659 HL
mghv-miR-M1-4 HL
hsa-miR-129- HL
5p/mmu-miR-129-
5p/rno-miR-129
ebv-miR-BART13 HL
hsa-miR-193b* HL
hsa-miR-422a HL
hsa-miR-503 HL
kshv-miR-K12-3 HL
hsa-miR-766 HL
hsa-miR-516a-5p HL
hsa-miR-125b- HL
1*/mmu-miR-125b-
3p/rno-miR-125b-3p
hsa-miR-149* HL
ebv-miR-BART6-3p HL
ebv-miR-BART19-3p HL
hsa-miR-671- HL
5p/mmu-miR-671-5p
ebv-miR-BART8* HL
hsa-miR-509-3-5p HL
hsa-miR-602 HL
ebv-miR-BHRF1-1 HL
mghv-miR-M1-7-3p HL
mghv-miR-M1-2 HL
hsa-miR-675 HL
ebv-miR-BHRF1-2 HL
hsa-miR-145*/mmu- HL
miR-145*
hsa-miR-296-5p/mmu- HL
miR-296-5p/rno-miR-
296*
hsa-miR-17*/rno-miR- HL
17-3p
hsa-miR-452 HL
hsa-miR-943 HL
hsa-miR-326/mmu-miR- HL
326/rno-miR-326
hsa-miR-652/mmu-miR- HL
652/rno-miR-652
hsa-miR-623 HL
hsa-miR-194* HL
hsa-miR-557 HL
hsa-miR-125a-3p/mmu- HL
miR-125a-3p/rno-miR-
125a-3p
hsa-miR-425*/mmu- HL
miR-425*
hsa-miR-10a*/mmu- HL
miR-10a*/rno-miR-10a-
3p
hsa-miR-323-3p/mmu- HL
miR-323-3p/rno-miR-
323
hsa-miR-519e HL
hsa-miR-502-5p HL
hsa-miR-124*/mmu- HL
miR-124*/rno-miR-124*
hsa-miR-345 HL
hsa-miR-584 HL
hsa-miR-654-5p HL
hsa-miR-331-5p/mmu- HL
miR-331-5p
hsa-miR-650 HL
hsa-miR-202 HL
hsa-miR-548b-3p HL
hsa-miR-492 HL
hsa-miR-135a*/mmu- HL
miR-135a*
ebv-miR-BART20-3p HL
hsa-miR-586 HL
hsa-miR-338-5p/mmu- HL
miR-338-5p/rno-miR-
338*
hsa-miR-92b* HL
hiv1-miR-H1 HL
hsa-miR-508-5p HL
hsa-miR-542-5p/mmu- HL
miR-542-5p/rno-miR-
542-5p
hsa-miR-490-5p HL
hsa-miR-663 HL
hsa-miR-433/mmu-miR- HL
433/rno-miR-433
