Monoclonal antibodies to the Mystery Swine Disease virus

Monoclonal antibody sera can be produced by hybridoma technique. After immunization of a mouse, pig, rat, rabbit or other appropriate species with Mystery Swine Disease (MSD) containing cell culture lysate, the spleen of the animal can be removed and converted into a whole cell preparation. Preferably the monoclonal antibody is produced by a murine derived hybrid cell line wherein the antibody is an IgG or IgM type immunoglobulin. Example monoclonal antibodies to the infectious agent for SIRS are monoclonal antibody SDOW 12 and SDOW 17.

Skip to:  ·  Claims  ·  References Cited  · Patent History  ·  Patent History

Claims

1. A monoclonal antibody produced by a murine-derived hybrid cell line wherein said antibody specifically binds to an epitope on a 15 kD antigen of viral agent ATCC VR-2332.

2. The monoclonal antibody of claim 1 wherein said antibody is an IgG or IgM type immunoglobulin molecule.

3. The monoclonal antibody of claim 1 wherein the antibody is produced by hybridoma cell line ATCC HB10996.

4. The monoclonal antibody of claim 1 wherein the antibody is produced by hybridoma cell line ATCC HB10997.

Referenced Cited
Foreign Patent Documents
145705 January 1981 DEX
8908701 September 1990 WOX
92/21375 December 1992 WOX
93/03760 March 1993 WOX
Other references
Patent History
Patent number: 5677429
Type: Grant
Filed: Jun 7, 1995
Date of Patent: Oct 14, 1997
Assignee: South Dakota State University (Brookings, SD)
Inventor: David A. Benfield (Brookings, SD)
Primary Examiner: Marian C. Knode
Assistant Examiner: DatQuan Lee
Law Firm: Merchant & Gould, Smith, Edell, Welter & Schmidt
Application Number: 8/488,286
Classifications
Current U.S. Class: 530/3883; Involving Virus Or Bacteriophage (435/5); Kit (435/975); 435/24027; 424/1471
International Classification: C07K 1610;