Patents Issued in August 14, 2003
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Publication number: 20030152891Abstract: A method and system for selectively staining dental composite materials includes an applicator (104) and a disclosing agent (102). In one embodiment, the applicator (104) provides the disclosing agent (102) to a tooth surface and a composite filling of the tooth. The composite filling is selectively stained to reveal the presence and location of the composite filling. In another embodiment, the tooth surface is selectively stained to reveal the presence and location of the composite filling.Type: ApplicationFiled: February 19, 2003Publication date: August 14, 2003Inventors: Paul C. Chiu, Catherine Hoffmann
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Publication number: 20030152892Abstract: An integrable naval virtual target range system and method provides three-dimensional graphical viewing capabilities of a virtual target range so that naval and fire support personnel can train together during both simulated and live fire exercises. The virtual target range system comprises a control subsystem having a computer system and a spotter subsystem for monitoring virtual impact points on the virtual target range. The target system may further comprise a buoy subsystem and/or an aerial vehicle subsystem, for use during live fire exercises, to locate ordnance impact points used for calculating the virtual impact points.Type: ApplicationFiled: February 11, 2002Publication date: August 14, 2003Applicant: United Defense, L.P.Inventors: Paul C. Huang, Norman S. Scott, Omar A. Khan, Albert Sleder, David C. Heim
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Publication number: 20030152893Abstract: A portable flight simulator is installed upon a conventional vehicle trailer for storage and transportation to various sites. The apparatus includes a folding video screen which when erected provides a relatively wide and long field of view for the operator of the simulator, rather than a relatively small and close video monitor display. The screen and video projector are immovably affixed to an operator cab or cockpit when the simulator apparatus is erected for operation, with cockpit movement and attached screen and projector being controlled by the operator in the cockpit. A conventional personal computer and flight simulator program provide the video signal for the projector, which projects the video program onto the screen. The operator reacts to the video program by using a control stick to control the orientation of the video program conventionally. Audio output may also be provided for realistic sound effects, or music for the operator.Type: ApplicationFiled: December 11, 2000Publication date: August 14, 2003Inventor: Allen G. Edgar
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Publication number: 20030152894Abstract: An automatic reading system provides a system and methods of evaluating a user's reading skills while the user is reading out loud. The automatic reading system adjusts text of an electronic book as the user is reading to increase or decrease a level profile of the electronic book. The automatic reading system also provides reading recommendations, feedback, and marketing data.Type: ApplicationFiled: February 6, 2002Publication date: August 14, 2003Applicant: Ordinate CorporationInventor: Brent Townshend
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Publication number: 20030152895Abstract: A system and method of correlating book leveling criteria to label leveled reading books is disclosed. An instructional reading level of a selected book leveling method is input into a data entry field of a graphical user interface such as at a personal computer or other processor. This instructional reading level is correlated with instructional reading levels of other book leveling methods to produce correlated instructional reading levels. One or more correlated instructional reading levels are selected to be printed on a label for application onto a reading book.Type: ApplicationFiled: February 11, 2002Publication date: August 14, 2003Inventors: Edwin C. Reisz, Jeffrey R. Brake
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Publication number: 20030152896Abstract: A kit of parts for use in potty training an infant, comprising: a plurality of sheets of toilet tissue carrying images and preferably on a roll (14); a plurality of training cards (30, 31, 32, 33, 34, 35) each carrying one of a sequence of pictures, the sequence illustrating an infant during different the stages of using a potty and then being rewarded with a badge; and a badge (20) or badges incorporating means, such as glue, to affix the badge to an item of clothing. The kit may be contained in a dispenser box (10) which may serve as a dispenser and storage container. The consumable items of the kit such as the toilet tissue and reward badges may be replaced as appropriate, particularly from a refill pack comprising a roll of toilet tissue (14) and a sheet (19) of self-adhesive badges.Type: ApplicationFiled: January 13, 2003Publication date: August 14, 2003Inventor: Caroline Hudson
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Publication number: 20030152897Abstract: A method for navigating a viewpoint of a virtual endoscope in a lumen of a structure is provided. The method includes the steps of (a)determining an initial viewpoint of the virtual endoscope, the initial viewpoint having a first center point and first direction; (b)determining a longest ray from the initial viewpoint to the lumen, the longest ray having a first longest ray direction; (c)determining a second direction between the first direction of the initial viewpoint and the first longest ray direction; (d)turning the viewpoint to the second direction and moving the initial viewpoint a first predetermined distance in a first direction of the initial viewpoint; (e)calculating a second center point of the viewpoint; (f)moving the viewpoint to the second center point; and repeating steps (b) through (f) until the viewpoint reaches an intended target.Type: ApplicationFiled: December 18, 2002Publication date: August 14, 2003Inventor: Bernhard Geiger
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Publication number: 20030152898Abstract: An apparatus for the education and entertainment of infants includes a housing that supports a plurality of objects over a crib or playpen and which rotates the objects in a circle. An audio program includes a recitation of each object when it is disposed proximate an infant disposed in the crib or playpen. Accordingly, the infant learns to associate the speech aspect of the audio presentation with the objects through a pattern of continual visual and synchronized simultaneous audio repetition and reinforcement, thereby optimally learning the names of objects and possibly even contributing to the learning of speech syntax.Type: ApplicationFiled: February 12, 2002Publication date: August 14, 2003Inventors: Stephan Erich Hills Strebl, Caroline Kim Chipman
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Publication number: 20030152899Abstract: An e-learning course may include a number of structural elements that are aggregated into one or more levels. Each higher level may refer to any instance of a lower level. The structural elements include a course, a sub-course, a learning unit, or a knowledge item. The structural elements may include metadata. The structural elements also may include relations between structural elements. The course and its structural elements do not enforce any sequence of structural elements that the learner may use to traverse the course. In addition, the structural elements may be reused and reassembled to form different courses. A content repository is configured to store course data and its structural elements. A content player is configured to access the stored course data and assemble the structural elements.Type: ApplicationFiled: May 31, 2002Publication date: August 14, 2003Inventors: Andreas Krebs, Joachim Schaper, Wolfgang Gerteis, Michael Altenhofen, Torsten Leidig, Norbert Meder
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Publication number: 20030152900Abstract: A learning system and method apply learning strategies to course structure. The course structure includes a plurality of structural elements and one or more relations that indicate dependences between the structural elements. A learning strategy is selected and applied to the course structure. A sequence of structural elements is determined based on the applied learning strategy. Course content associated with the structural elements is suggested to be presented to the learner based on the determined sequence of structural elements. The learner may select the learning strategy. The learning strategies include macro-strategies and micro-strategies, both of which may be applied to the same course structure.Type: ApplicationFiled: May 31, 2002Publication date: August 14, 2003Inventors: Andreas Krebs, Joachim Schaper, Wolfgang Gerteis, Michael Altenhofen, Torsten Leidig, Norbert Meder
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Publication number: 20030152901Abstract: A repository manager and copy editor may be used to create an offline course, which allow learners to process course material locally on their computer without the need to connect to the e-learning system. A generic packaging list is created when the course is offered offline. A specific packaging list customized for the learner is created when the offline course is downloaded. An offline manager tool provides an interface for taking the course offline.Type: ApplicationFiled: July 31, 2002Publication date: August 14, 2003Inventors: Michael Altenhofen, Wolfgang Theilmann, Andreas S. Krebs
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Publication number: 20030152902Abstract: An offline learning system may include a learning management system, a content management system, and a learning administration system. The content management system is provided with a metadata provider, a learning content storage (e.g., the content repository), and a repository manager. In addition, the learning station includes an offline manager tool and a storage for storing an offline learning account, metadata, and course. Offline courses are derived from the published on-line courses. A copy editor may create offline versions of courses from published courses using a repository manager tool. The repository tool manager may be used to create a packaging list for the offline version of a course. The packaging list includes access paths and metadata of all files that are part of the course. The packaging list may be used to determine which files need to be downloaded to the learning station to allow the learner to work offline.Type: ApplicationFiled: July 31, 2002Publication date: August 14, 2003Inventors: Michael Altenhofen, Wolfgang Theilmann, Andreas S. Krebs
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Publication number: 20030152903Abstract: A learning system may be used to dynamically create courses tailored to the needs of a learner. In one implementation, the learning system may dynamically create, assemble, and/or build new temporary courses from the structural elements of an existing course according to the specific needs of a learner. The learner's needs may be expressed using a budget and other criteria provided by the learner. Using the budget and criteria, the learning system may identify existing structural elements that may be used to assemble a dynamic course. After the course is assembled, the course may be temporarily stored and presented to the learner using the learning station.Type: ApplicationFiled: November 8, 2002Publication date: August 14, 2003Inventor: Wolfgang Theilmann
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Publication number: 20030152904Abstract: A network based educational system is described. The system generally includes a student interface, a classroom interface and an administrative interface. Synchronous and asynchronous applications are used, typically with streaming video to deliver courseware to learners on the system.Type: ApplicationFiled: November 29, 2002Publication date: August 14, 2003Inventor: Thomas R. Doty
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Publication number: 20030152905Abstract: A learning system may include an interface, a content repository, and a content player. The interface is configured to receive a learning strategy that is selected by a learner. The content repository is configured to store course data. The content player is configured to access the stored course data and to apply a selected learning strategy to the course data to determine course data to be suggested for presentation to the learner. The content repository may be configured to store structural elements of a course that include a course, a sub-course, a learning unit, or a knowledge item. The course and its structural elements do not enforce any sequence of structural elements that the learner may use to traverse the course. The content player may apply the learning strategy to the course to generate a suggested sequence of structural elements to the user. The learning strategy may include a macro-strategy and a micro-strategy.Type: ApplicationFiled: April 30, 2002Publication date: August 14, 2003Inventors: Michael Altenhofen, Joachim Schaper, Wolfgang Gerteis, Andreas Krebs, Torsten Leidig
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Publication number: 20030152906Abstract: A learning system, method, and software generate a navigation path through a course based on a learning strategy. The course may be navigated by receiving graphs corresponding to the course, applying the learning strategy to the graphs, and generating a navigation path through the course for the learner based on the applied strategy. The navigation path may be used to suggest content from the course for presentation to a learner.Type: ApplicationFiled: April 30, 2002Publication date: August 14, 2003Inventors: Andreas Krebs, Wolfgang Gerteis, Michael Altenhofen
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Publication number: 20030152907Abstract: This invention combines God's gift of reincarnation with love resulting in immortality.Type: ApplicationFiled: February 12, 2002Publication date: August 14, 2003Inventor: George Knauer
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Publication number: 20030152908Abstract: A foldable writing board made up of at least four panels attached by hinges or other attachment means so that the board can be folded out flat to become a full size writing board, folded in two to become either a taller or shorter noteboard (folded in a tent-like configuration) or folded in four to either store flat or to be set up as a small computer notepad. The panels are held together by three or more foldable attachment means that hold the panels together, but allow them to also fold along adjacent edges.Type: ApplicationFiled: February 13, 2002Publication date: August 14, 2003Inventor: Oscar Lopez
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Publication number: 20030152909Abstract: Micro-organ cultures which include isolated populations of cells having specific characteristics are described. Salient features of the subject micro-organ cultures include the ability to be maintained in culture for relatively long periods of time, as well as the preservation of an organ microarchitecture which facilitates, for example, cell-cell and cell-matrix interactions analogous to those occurring in the source organ. The micro-organ cultures of the invention can be used in methods for delivering gene products to recipient subjects, for identifying cell proliferative and cell differentiating agents, and identification and isolation of progenitor and stem cells. In addition, the micro-organ cultures of the present invention can be used in methods for identifying inhibitors of cell proliferation, cell differentiation and viral infectivity. In other embodiments, the micro-organ cultures can be used for transplantation.Type: ApplicationFiled: December 17, 2002Publication date: August 14, 2003Inventor: Eduardo N. Mitrani
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Publication number: 20030152910Abstract: The present invention relates to a method for the identification of kinase inhibitors which is suitable for high-throughput screening. Moreover, the inhibitory effect of test substances can be quantified and a potential cytotoxicity of the respective inhibitors can be detected. The method is particularly suitable for the identification of inhibitors of viral kinases, e.g. herpes viral kinases.Type: ApplicationFiled: October 25, 2002Publication date: August 14, 2003Inventors: Matthias Stein-Gerlach, Helmut Mett, Manfred Marschall, Thomas Stamminger
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Publication number: 20030152911Abstract: Methods for determining chronic hepatitis C infection are provided by the present invention. The methods generally involve determining the optical density of fluid samples which have tested positive for hepatitis C infection using an antibody-based assay. The optical density can then be used to determine in a predictive or probability-based manner whether the fluid sample contains chronic hepatitis C infection or whether the fluid sample merely contains antibodies to hepatitis C infection without resorting to costly molecular detection methods. Thus, the present invention permits differentiation between individuals who have cleared hepatitis C infection but still test positive in an antibody-based assay from individuals having chronic hepatitis C infection.Type: ApplicationFiled: January 18, 2002Publication date: August 14, 2003Inventor: Robert L. Stout
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Publication number: 20030152912Abstract: Methods and compositions are provided for screening candidate antiviral agents using cells containing subgenomic viral replication systems such as replicons and minigenomes. The methods involve the simultaneous assay of more than one subgenomic viral replication system. Compositions useful for these methods are also provided.Type: ApplicationFiled: January 29, 2002Publication date: August 14, 2003Inventors: Julie Dyall, Charles P. Romano, Paul D. Olivo, Robert M. Roth
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Publication number: 20030152913Abstract: Compositions are provided that comprise antibody against coreceptors for human immunodeficiency virus such as CCR5 and CXCR4. In particular, monoclonal human antibodies against human CCR5 are provided that bind to CCR5 with high affinity and are capable of inhibiting HIV infection at low concentrations. The antibodies can be used as prophylactics or therapeutics to prevent and treat HIV infection, for screening drugs, and for diagnosing diseases or conditions associated with interactions with HIV coreceptors.Type: ApplicationFiled: February 8, 2002Publication date: August 14, 2003Inventors: Shaobing Hua, Michelle H. Pauling, Li Zhu
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Publication number: 20030152914Abstract: Sequences are provided that are capable of directing circular adeno-associated virus replication, useful in vectors for providing therapeutic agents to a subject in need thereof. The vectors of the invention are particularly useful in the treatment of acute medical conditions requiring rapid gene expression. Further provided are methods for producing packaged defective viral vectors.Type: ApplicationFiled: May 31, 2002Publication date: August 14, 2003Inventors: Michael G. Kaplitt, Sergei Moussatov
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Publication number: 20030152915Abstract: A recombinant RNA-dependent RNA polymerase of hepatitis C virus (r-HCV-RDRP) coding DNA was cloned and expressed yielding active enzyme in vitro. The r-HCV-RDRP can include up to 20 added amino acids and up to nine deleted or substituted amino acids at the NH2-terminus of the encoded amino acid sequence. The invention provides method to solubilize r-HCV-RDRP from a host cell lysate and purified r-HCV-RDRP. Methods for screening for inhibitors of r-HCV-RDRP in vitro, for making stably transfected mammalian cells expressing r-HCV-RDRP and for in vivo testing of r-HCV-RDRP inhibitors in vivo are disclosed. The invention provides antibodies to r-HCV-RDRP and methods for detecting antibodies to HCV-RDRP in serum of human patients.Type: ApplicationFiled: September 12, 2002Publication date: August 14, 2003Inventor: Curt H. Hagedorn
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Publication number: 20030152916Abstract: The present invention relates to oligonucleotides for use in amplifying and detecting HIV nucleic acid in a sample.Type: ApplicationFiled: September 16, 2002Publication date: August 14, 2003Inventors: Daniel L. Kacian, Timothy J. Fultz, Sherrol H. McDonough
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Publication number: 20030152917Abstract: The present invention is drawn to a method of managing HIV chemotherapy of patients who are HIV positive, by transfecting a cell line susceptible to infection by HIV with a sequence from the pol gene of HIV, which sequence encodes at least one desired target enzyme selected from integrase, protease or reverse transcriptase, obtained by isolating viral RNA from a sample of a biological material from a patient and reverse transcribing the desired region of the pol gene, and a HIV-DNA construct that lacks a sequence encoding the desired target enzyme, culturing the transfected cells so as to create a stock of chimeric viruses, assessing the phenotypic sensitivity of the chimeric viruses to at least one inhibitor of the target enzyme encoded by the pol gene of HIV, and assigning a value thereto, constructing a data set containing the value for chimeric virus sensitivity and the corresponding value for a chimeric wild-type strain of HIV, repeating the sensitivity assessment for at least two further inhibitors andType: ApplicationFiled: January 15, 2003Publication date: August 14, 2003Applicant: VIRCO N. V.Inventors: Marie-Pierre de Bethune, Kurt Hertogs, Rudi Pauwels
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Publication number: 20030152918Abstract: Primer sequences and materials are pre-prepared as test kits for enabling appropriate gene scanning patterns, preferably by two dimensional electrophoresis (TDGS), for use in detecting sequence variations and/or mutations in BRCA1 and h MLH1 genesType: ApplicationFiled: May 6, 1999Publication date: August 14, 2003Inventor: JAN VIJG
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Publication number: 20030152919Abstract: Methods are provided for diagnosing and/or characterizing chronic immune disease activity in a subject. In the subject methods, a sample is obtained from a subject suspected of having or known to have a chronic immune disease. The sample is then assayed for the presence of low molecular actin fragments. The assay results are used to diagnose the presence of chronic immune disease activity and/or characterize chronic immune disease activity in the subject, e.g. to confirm an initial chronic immune disease diagnosis, to determine the stage of the disease, to monitor disease progression, to predict disease attacks, and the like. Also provided by the subject invention are kits for practicing the methods.Type: ApplicationFiled: May 15, 2000Publication date: August 14, 2003Inventors: Simon Adriaan Michiel Roelens, Patrick Englebienne, Anne Marie Yvonne Robert D'Haese, C. V. Taylor Herst
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Publication number: 20030152920Abstract: Hydrodynamic enhanced dielectrophoretic particle trapping carried out by introducing a side stream into the main stream to squeeze the fluid containing particles close to the electrodes producing the dielelectrophoretic forces. The region of most effective or the strongest forces in the manipulating fields of the electrodes producing the dielectrophoretic forces is close to the electrodes, within 100 &mgr;m from the electrodes. The particle trapping arrangement uses a series of electrodes with an AC field placed between pairs of electrodes, which causes trapping of particles along the edges of the electrodes. By forcing an incoming flow stream containing cells and DNA, for example, close to the electrodes using another flow stream improves the efficiency of the DNA trapping.Type: ApplicationFiled: March 28, 2001Publication date: August 14, 2003Applicant: The Regents of the University of CaliforniaInventor: Robin R. Miles
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Publication number: 20030152921Abstract: The invention concerns GENSET polynucleotides and polypeptides. Such GENSET products may be used as reagents in forensic analyses, as chromosome markers, as tissue/cell/organelle-specific markers, in the production of expression vectors. In addition, they may be used in screening and diagnosis assays for abnormal GENSET expression and/or biological activity and for screening compounds that may be used in the treatment of GENSET-related disorders.Type: ApplicationFiled: June 8, 2001Publication date: August 14, 2003Inventors: Jean-Baptiste Dumas Milne Edwards, Lydie Bougueleret, Severin Jobert
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Publication number: 20030152922Abstract: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.Type: ApplicationFiled: July 13, 2001Publication date: August 14, 2003Applicant: Genentech, Inc.Inventors: Avi Ashkenazi, David Botstein, Luc Desnoyers, Dan L. Eaton, Napoleone Ferrara, Ellen Filvaroff, Sherman Fong, Wei-Qiang Gao, Hanspeter Gerber, Mary E. Gerritsen, Audrey Goddard, Paul J. Godowski, J. Christopher Grimaldi, Austin L. Gurney, Kenneth J. Hillan, Ivar J. Kljavin, Jennie P. Mather, James Pan, Nicholas F. Paoni, Margaret Ann Roy, Timothy A. Stewart, Daniel Tumas, P. Mickey Williams, William I. Wood
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Publication number: 20030152923Abstract: The overexpression of certain marker genes including Wnt5a has been found useful in the identification of more aggressive forms of malignant melanoma. Therefore, the overexpression of these genes in tumor samples of malignant melanoma may be useful in the diagnosis, profiling, and treatment of patients suffering from this disease. Inhibitors of Wnt5a activity may be useful in the treatment of aggressive forms of malignant melanoma. Inhibition of Wnt5a activity may be effected by any method including anti-sense therapy, gene therapy, and pharmaceutical intervention.Type: ApplicationFiled: August 2, 2001Publication date: August 14, 2003Inventors: Zohar Yakhini, Michael Bittner, Jeff Trent, Amir Ben-Dor, Paul Meltzer, Yidong Chen, Ashani Weeraratna, Yuan Jiang, Nick Sampas, Edward Dougherty
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Publication number: 20030152924Abstract: Methods are provided for the determination of specific nucleotide sequences, particularly single nucleotide polymorphisms, by using probes comprising a first strand complementary to the target sequence, a shorter second strand forming a stem and a linker adjacent the double stranded stem connecting the two strands, whereby binding of target to the probe under conditions that do not cause melting of the double stranded stem in the absence of target results in the dissociation of the second strand from the first strand. The ss second strand is then detected as exemplified by using a FRET pair, where dissociation of the stem results in separation of the FRET pair and increase in fluorescence. Amplification of the target sequence may be employed prior to combination with the probe. The method finds particular application with complex nucleic acid mixtures.Type: ApplicationFiled: December 7, 2001Publication date: August 14, 2003Inventors: Edwin F. Ullman, Ming Wu
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Publication number: 20030152925Abstract: The present invention is directed to a novel annealing control primer system (“ACP system”), for regulating primer annealing specificity during polymerase chain reaction (PCR). The principle of the ACP system is based on the composition of an oligonucleotide primer having 3′- and 5′-end distinct portions separated by a deoxyinosine group, which is a unique feature of this invention. The present invention also provides a process using the ACP system for performing two stage PCR amplifications to selectively amplify a target nucleic acid fragment from a nucleic acid or a mixture. The present invention also provides a method using the ACP system for detecting and cloning differentially expressed mRNAs in two or more nucleic acid samples. Kits containing ACP are included within the scope of the present invention. Furthermore, the present invention can be adapted to almost unlimited application in all fields of PCR-based technology.Type: ApplicationFiled: December 14, 2001Publication date: August 14, 2003Inventor: Jong-Yoon Chun
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Publication number: 20030152926Abstract: Described herein are methods and compositions that can be used for diagnosis and treatment of angiogenic phenotypes and angiogenesis-associated diseases. Also described herein are methods that can be used to identify modulators of angiogenesis.Type: ApplicationFiled: December 6, 2001Publication date: August 14, 2003Applicant: Eos Biotechnology, Inc.Inventors: Richard Murray, Richard Glynne, Susan R. Watson
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Publication number: 20030152927Abstract: The invention relates to novel analysis slides or substrates with a closed configuration. The slides may be used for any application which normally utilizes a conventional microscope slide and can be used in conjunction with any type of equipment typically used to manipulate or evaluate a standard microscope slide. In particular, the invention provides closed slides for covalent immobilization of polypeptides and nucleic acids. Further provided are methods for carrying out biological assays using arrays of biomolecules immobilized on the slides of the invention. The arrays of the invention are comprised of a unique meandering or straight channel design as compared to the rectangular design of conventional arrays.Type: ApplicationFiled: October 25, 2001Publication date: August 14, 2003Inventors: Mogens Havsteen Jakobsen, Lars Kongsbak
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Publication number: 20030152928Abstract: The present invention provides a method of screening genes based on expression information differing from gene expression information obtainable by DNA chip/DNA microarray techniques. The method of screening genes comprises performing in situ hybridization in respect of a tissue or cell sample from an organism using a probe which specifically hybridizes with mRNA and/or expression sequence tag being a product of gene expression, and examining localization of the mRNA and/or expression sequence tag in the tissue or cell.Type: ApplicationFiled: January 8, 2002Publication date: August 14, 2003Inventors: Satoshi Asai, Toshihito Nagata, Yasuo Takahashi, Yukimoto Ishii, Koichi Ishikawa
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Publication number: 20030152929Abstract: An apparatus for identifying an unknown DNA sample. The apparatus includes a plurality of detection nodes, each of which is operable for allowing an interaction between a known DNA sample and an unknown DNA sample, and for generating an output signal if hybridization occurs between the known DNA sample and the unknown DNA sample. The apparatus further includes a decoder operative for receiving an input signal indicative of which of the plurality of detection nodes should be selected for processing and for outputting control signals which operate to activate the selected detection node. Further, each of the detection nodes comprises a first floating gate transistor having a conductance value which varies if hybridization occurs between the known DNA sample and the unknown DNA sample contained in the first transistor. This change of conductance value is utilized to generate the output signal which indicates that hybridization has occurred.Type: ApplicationFiled: February 11, 2002Publication date: August 14, 2003Applicant: Matsushita Electric Industrial Co., Ltd.Inventor: John K. Howard
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Publication number: 20030152930Abstract: An apparatus for identifying an unknown DNA sample. The apparatus includes a plurality of detection nodes, each of which is operable for allowing an interaction between a known DNA sample and an unknown DNA sample, and for generating an output signal if hybridization occurs between the known DNA sample and the unknown DNA sample. The apparatus further includes a decoder operative for receiving an input signal indicative of which of the plurality of detection nodes should be selected for processing and for outputting control signals which operate to activate the selected detection node. Further, each of the detection nodes comprises a first capacitor having a capacitance value which varies if hybridization occurs between the known DNA sample and the unknown DNA sample contained in the first capacitor. This change of capacitance is utilized to generate the output signal which indicates that hybridization has occurred.Type: ApplicationFiled: February 11, 2002Publication date: August 14, 2003Applicant: Matsushita Electric Industrial Co., Ltd.Inventor: John K. Howard
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Publication number: 20030152931Abstract: The present invention relates to a nucleic acid detection device. The present invention especially relates to a nucleic acid detection device utilizing the novel concept of multiple probes to one target. The device comprises a solid support, on which at least one probe set is attached. The probe set comprises multiple types of oligonucleotide probes, wherein each probe has a unique sequence complementary to the different target nucleic acid. The present invention also relates to a method for detecting nucleic acid. The method involves carrying out a hybridization reaction of the previously described device and a sample in order to determine the nucleic acids present in the sample.Type: ApplicationFiled: February 11, 2002Publication date: August 14, 2003Inventors: Chung-Fan Chiou, Chuan-Hsiung Chang, Cheng-Tao Wu, Yao-Sung Chang, Jie-Len Huang, Chao-Chi Pan
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Publication number: 20030152932Abstract: Disclosed are compositions and methods for detecting small quantities of analytes such as proteins and peptides. The method involves associating a DNA circle with the analyte and subsequent release and rolling circle replication of the circular DNA molecule. In the method, an amplification target circle is associated with analytes using a conjugate of the circle and a specific binding molecule that is specific for the analyte to be detected. Amplification target circles not associated with the proteins are removed, the amplification target circles that are associated with the proteins are decoupled from the specific binding molecule and amplified by rolling circle amplification. The amplification is isothermic and can result in the production of a large amount of nucleic acid from each primer. The amplified DNA serves as a readily detectable signal for the analytes.Type: ApplicationFiled: February 8, 2002Publication date: August 14, 2003Inventors: Gyanendra Kumar, Patricio Abarzua, Michael Egholm
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Publication number: 20030152933Abstract: The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating diseases, disorders, and/or conditions related to these novel human secreted proteins.Type: ApplicationFiled: February 12, 2002Publication date: August 14, 2003Inventors: Steven C. Barash, Jian Ni, Steven M. Ruben, Craig A. Rosen, Yanggu Shi
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Publication number: 20030152934Abstract: The invention discloses a device for hybridization reaction between a target molecule in a fluid and a probe, which comprises a microfluidic channel comprising a first portion and a second portion following said first portion; wherein said first portion have an irregular cross section and said second portion has a probe, and a fluid driving element connected the ends of said channel with tubes, wherein said fluid element can move said target molecules back-and-forth for repeatedly passing through said second portion.Type: ApplicationFiled: February 11, 2002Publication date: August 14, 2003Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTEInventors: Yao-Sung Chang, Yung-Chiang Chung, Ming-Zheng Shiu
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Publication number: 20030152935Abstract: The present invention provides methods and compositions for screening, diagnosis and prognosis of breast cancer, for monitoring the effectiveness of breast cancer treatment, and for drug development. Breast Cancer-Associated Features (BFs), detectable by two-dimensional electrophoresis of serum are described. The invention further provides Breast Cancer-Associated Protein Isoforms (BPIs) detectable in cerebrospinal fluid, serum or plasma, preparations comprising isolated BPIs, antibodies immunospecific for BPIs, and kits comprising the aforesaid.Type: ApplicationFiled: February 13, 2002Publication date: August 14, 2003Inventor: Herath Mudiyanselage Athula Chandrasiri Herath
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Publication number: 20030152936Abstract: The subject invention relates to methods for detection of mutations in a PKD gene using DHPLC. The invention includes the following aspects: identification of PKD unique sites; design of PKD-specific primers; amplification of PKD-specific products; and analysis of PCR amplified products by DHPLC. The invention further relates to compositions such as identified unique sites and PKD-specific primers, and kits for performing the methods of the invention.Type: ApplicationFiled: February 26, 2002Publication date: August 14, 2003Applicant: Athena DiagnosticsInventors: Jeffrey George Jones, Aidan Noel Hennigan, John A. Curran, Susan Kimberly Allen, Normand J. Robichaud, Jing Wang, Kerry Ellen Flynn, Jorge A. Garces, Christopher M. Palatucci
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Publication number: 20030152937Abstract: The discriminating capability of hybridization assays is increased by a combination of labelled primers which produce amplificates of one strand of a nucleic acid with a capture probe which is complementary to the same strand of the nucleic acid.Type: ApplicationFiled: March 22, 2002Publication date: August 14, 2003Applicant: ROCHE DIAGNOSTICS GmbHInventors: Kurt Weindel, Joachim Brand
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Publication number: 20030152938Abstract: The present invention relates to an RET oligonucleotide microchip for detecting mutation in the mutational hot spot regions of RET gene, a manufacturing process thereof and a method for detecting hereditary cancer employing same, wherein specific oligonucleotides are selectively designed to detect various missense mutations at the mutational hot spots of RET gene.Type: ApplicationFiled: April 5, 2002Publication date: August 14, 2003Inventors: Jae-Gahb Park, Il-Jin Kim, Hio Chung Kang, Jae-Hyun Park
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Publication number: 20030152939Abstract: The present invention provides novel isolated polypeptides, a fragment of any of them, polynucleotides encoding them and antibodies that immunospecifically bind to them. The invention additionally provides several methods in which these proteins, polynucleotides and antibodies are used in methods of detection and methods of treatment are also disclosed. These methods of detection and treatment are directed to a broad range of pathological states.Type: ApplicationFiled: April 9, 2002Publication date: August 14, 2003Inventors: Glennda Smithson, Bryan D. Zerhusen, Mei Zhong, Nikolai V. Khramtsov, Li Li, Vladimir Y. Gusev, Muralidhara Padigaru, David W. Anderson, Richard A. Shimkets
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Publication number: 20030152940Abstract: The current invention relates to vectors and methods for efficient expression of HCV envelope proteins in eukaryotic cells. More particularly said vectors comprise the coding sequence for an avian lysozyme signal peptide or a functional equivalent thereof joined to a HCV envelope protein or a part thereof. Said avian lysozyme signal peptide is efficiently removed when the protein comprising said avian lysozyme signal peptide joined to a HCV envelope protein or a part thereof is expressed in a eukaryotic cell. Suitable eukaryotic cells include yeast cells such as Saccharomyces or Hansenula cells.Type: ApplicationFiled: April 24, 2002Publication date: August 14, 2003Inventors: Erwin Sablon, Annie Van Broekhoven, Fons Bosman, Erik Depla, Geert Deschamps