Patents Issued in July 1, 2004
  • Publication number: 20040126766
    Abstract: The present invention provides for assaying as few as a single microarray of a chemical array of microarrays at a time without contamination or damage to a remainder of the microarrays. A chemical array apparatus, system and method comprise a microarray attached to a surface of a planar substrate and a breakaway seal or barrier applied to the substrate surface to surround the microarray. The breakaway seal provides the microarray fluid isolation and is manipulatable. The system further comprises a cover extending over and in contact with the breakaway seal to cover the microarray. The method further comprises processing a microarray of the chemical array with a fluid, and breaking away a portion of the breakaway seal to create a gap. The gap releases the fluid in a direction away from remaining microarrays of the chemical array. A removable cover overlies a microarray on the chemical array apparatus.
    Type: Application
    Filed: December 26, 2002
    Publication date: July 1, 2004
    Inventor: Douglas A. Amorese
  • Publication number: 20040126767
    Abstract: The present invention relates to methods and system for the diagnosis diseases or conditions. In a particular aspect, a disclosed method for determining a panel includes calculating a panel response for each patient in a set of diseased patients and in a set of non-diseased patients. The panel response is a function of the value of each of a plurality of markers in a panel of markers. The method also includes calculating a value for an objective function. The objective function is indicative of the effectiveness of the panel. The steps of calculating a panel response for each patient and calculating a value for an objective function are iterated by varying at least one of the parameters relating to the panel response function and a sense of each marker to facilitate optimization of the objective function. The objective function may be a measure of an overlap of panel responses of diseased patients and panel responses of non-diseased patients.
    Type: Application
    Filed: December 27, 2002
    Publication date: July 1, 2004
    Applicant: Biosite Incorporated
    Inventors: Joseph Michael Anderberg, Kenneth F. Buechler, Paul H. McPherson, Howard J. Kirchick, Jeffrey R. Dahlen
  • Publication number: 20040126768
    Abstract: Transgenic cells may be selected using temperature sensitive marker proteins. In this method, a population of host cells are transformed with a foreign DNA construct which includes at least one first nucleic acid coding sequence and a second nucleic acid sequence encoding a temperature sensitive marker protein, wherein each of the first and second nucleic acid coding sequences are operatively linked to gene expression control sequences. Suitable temperature sensitive marker proteins which may be used herein include heat shock proteins, heat shock transcription factors, cold regulated proteins (COR), or cold regulated protein transcription factors. Following transformation, the population of cells are cultured under temperature conditions wherein growth of non-transformed cells is suppressed or prevented while growth of cells transformed with the DNA construct is supported or promoted.
    Type: Application
    Filed: December 30, 2002
    Publication date: July 1, 2004
    Inventors: John J. Burke, Patrick J. O'Mahony, Jeffrey P. Velten, Melvin J. Oliver
  • Publication number: 20040126769
    Abstract: The invention is directed to binding agents having binding loops and a stable beta barrel conformation. The binding loops of these agents can easily be altered so that the binding agent can bind any selected target molecule. A variety of methods for generating binding agents with different binding loops are also provided.
    Type: Application
    Filed: December 30, 2002
    Publication date: July 1, 2004
    Inventor: Stephen Quirk
  • Publication number: 20040126770
    Abstract: Disclosed are compositions and methods for amplification of RNA molecules. The disclosed method involves synthesizing first strand cDNA molecules from RNA molecules, circularizing the first strand cDNA molecules and replicating the circularized first strand cDNA molecules using rolling circle replication. The method can be aided by the use of specialized primers for cDNA synthesis and specialized probes for circularizing the first strand cDNA molecules. The method can be used to replicate and amplify multiple RNA molecules, such as all RNA molecules in a sample or all mRNA molecules in a sample, or be used to replicate and amplify specific RNA molecules. Rolling circle replication of the circularized first strand cDNA molecules results in long DNA strands containing tandem repeats of the cDNA sequence. The tandem sequence DNA can be used directly (for detection of sequences, for example), further amplified, or used any other purpose.
    Type: Application
    Filed: December 31, 2002
    Publication date: July 1, 2004
    Inventors: Gyanendra Kumar, Patricio Abarzua
  • Publication number: 20040126771
    Abstract: A circularly permuted chimeric pRNA molecule carrying a stabilized biologically active RNA, such as a ribozyme.
    Type: Application
    Filed: February 24, 2003
    Publication date: July 1, 2004
    Applicant: Purdue Research Foundation
    Inventors: Peixuan Guo, Stephen M. Hoeprich, Dan Shu
  • Publication number: 20040126772
    Abstract: The present invention is related to a method for MALDI-TOF-MS analysis and/or sequencing of oligoribonucleotides. The present invention is also related to a method for determining the DNA nucleotide sequence using MALDI-TOF-MS, and a method for determining polymorphism using MALDI-TOF-MS. The present invention provides a kit for analyzing and/or sequencing a DNA template or a RNA transcription product and/or for determining polymorphism by MALDI-TOF-MS.
    Type: Application
    Filed: June 6, 2003
    Publication date: July 1, 2004
    Inventors: Yoshihide Hayashizaki, Tetsuyoshi Ono
  • Publication number: 20040126773
    Abstract: Assay systems that sense assay conditions using coded sensor particles.
    Type: Application
    Filed: May 23, 2003
    Publication date: July 1, 2004
    Inventors: Oren E. Beske, Simon Goldbard
  • Publication number: 20040126774
    Abstract: The present invention provides methods and compositions for generating novel nucleic acid molecules through targeted spliceosomal mediated trans-splicing. The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans-splicing reaction resulting in the generation of a novel chimeric RNA molecule (chimeric RNA). In particular, the PTMs of the present invention are genetically engineered to interact with factor VIII (FVIII) target pre-mRNA so as to result in correction of clotting FVIII genetic defects responsible for hemophilia A. The compositions of the invention further include recombinant vector systems capable of expressing the PTMs of the invention and cells expressing said PTMs.
    Type: Application
    Filed: June 5, 2003
    Publication date: July 1, 2004
    Inventors: LIoyd G. Mitchell, S. Gary Mansfield
  • Publication number: 20040126775
    Abstract: The present invention includes a method for diagnosing cancer and predicting recurrent cancer comprising detecting the presence of survivin in the biological fluid of a patient. The present invention also provides kits comprising one or more agents that detect survivin polypeptide or survivin nucleic acid and a container for collecting biological fluid for testing.
    Type: Application
    Filed: June 18, 2003
    Publication date: July 1, 2004
    Inventors: Dario C. Altieri, Robert M. Weiss, Shannon D. Smith, Marcia A. Wheeler, Victor A. Morris, Janet Plescia
  • Publication number: 20040126776
    Abstract: The invention relates to a method for producing a gene library, preferably a cDNA gene library and to a gene library that can be obtained by said method.
    Type: Application
    Filed: February 26, 2004
    Publication date: July 1, 2004
    Inventors: Michael Hofer, Martin Hofmann, Carmen Kaiser, Harald Kranz, Ralf Lobbert
  • Publication number: 20040126777
    Abstract: Isolated nucleic acid molecules encoding polypeptides from a human, reagents related thereto (including purified polypeptidesspecific antibodies) are provided. Methods of using said reagentsand diagnostic kits are also provided.
    Type: Application
    Filed: July 25, 2003
    Publication date: July 1, 2004
    Inventors: Ramesh Rajani Bhatt, John Nels Calley, Josef Georg Heuer, Gerald Patrick Keleher, Joanne Sloan Lancaster, Qingqin Li, Deshun Lu, Bradley Jay Mills, Santosh Kumar Mishra, Douglas Raymond Perkins, Scott William Rowlinson, Rosamund Carol Smith, Eric Wen Su, He Wang, Yu Zhi
  • Publication number: 20040126778
    Abstract: The present invention relates to a process for separating nucleic acid molecules, preferably open circular and super-coiled plasmid DNA and RNA molecules from each other, comprising the steps of providing a solution comprising the molecules; adsorbing the molecules to adsorbing groups on a carrier; and optionally washing the column with a suitable solution. The present process is especially suitable for large-scale isolation of supercoiled ccc DNA to be used in gene therapy.
    Type: Application
    Filed: September 25, 2003
    Publication date: July 1, 2004
    Inventors: Raf Lemmens, Tomas Nyhammar, Jan Berglof, Joachim Stadler
  • Publication number: 20040126779
    Abstract: The invention concerns a component for biological or biochemical analysis microsystems formed from a support and having at least one chemically functionalised surface zone, in order to allow in said zone the formation of a chemistry for anchoring biological or biochemical elements, and/or electrically, in order to allow in said zone the formation of electrical charges. The support comprises at least one part (21) formed of a composite material, said composite material being a mixture of at least one inert material and at least one chemically and/or electrically functionalisable material to provide said functionalised surface zone.
    Type: Application
    Filed: October 20, 2003
    Publication date: July 1, 2004
    Inventors: Philippe Combette, Frederic Revol-Cavalier, Frederique Mittler, Bernard Beneyton
  • Publication number: 20040126780
    Abstract: The invention relates to a method for determining luminescent molecules by means of optical excitation in confocal measurement volumes using a diffractive optical element. The method is particularly suitable for single-molecule determination, e.g. by means of fluorescence correlation spectroscopy or by means of dynamic light scattering. An apparatus suitable for carrying out the method according to the invention is furthermore disclosed.
    Type: Application
    Filed: December 1, 2003
    Publication date: July 1, 2004
    Inventors: Rudolf Rigler, Hans Blom, Mathias Johansson, Hard Sverker
  • Publication number: 20040126781
    Abstract: Method of inhibiting and diagnosing spontaneous abortion in a subject are provided. The subject methods are based, inter alia, on the administration of an agent that inhibits a costimulatory signal in a T cell such that spontaneous abortion in the subject is inhibited. The subject methods are also based on the levels of adhesion molecules, inflammatory cytokines, and immune cell surface molecules which are altered in spontaneous abortion.
    Type: Application
    Filed: June 27, 2003
    Publication date: July 1, 2004
    Applicant: GENETICS INSTITUTE, INC.
    Inventors: Vincent Ling, Gary S. Gray, James C. Keith, Srinivas Maganti
  • Publication number: 20040126782
    Abstract: A system and methods for evaluating genetic information and biological data applying a clustering approach which may be used for allele calling and genotyping. Statistical analysis of sample data is performed at various levels to develop a model which associates individual data points with selected genotyping clusters and provides a relative indication of the call confidence. The methods provide a unified framework for allele-calling in many different contexts and may be applied to the data acquired from various identification methodologies.
    Type: Application
    Filed: June 30, 2003
    Publication date: July 1, 2004
    Inventors: David P. Holden, XiaoPing Zhang, Daniel B. Allison, Austin B. Tomaney
  • Publication number: 20040126783
    Abstract: A method for preparing a nucleic acid component of a sample for amplification includes contacting the sample with a porous support that deactivates a nucleic acid amplification inhibitor component of the sample and directing a fluid through the porous support, whereby the nucleic acid component of the sample is directed through at least a portion of the porous support and is separated from the support, thereby preparing the nucleic acid component for amplification. The sample is separated from raw sample components through means that include a magnetic substrate. An apparatus suitable for conducting the method of the invention also is described.
    Type: Application
    Filed: July 10, 2003
    Publication date: July 1, 2004
    Applicants: Massachusetts Institute of Technology, Smiths Detection-Edgewood, Inc.,
    Inventors: Laura T. Bortolin, Lalitha Parameswaran, James Harper, Johanna Bobrow, Mark A. Hollis, Drew Chapman Brown, Eric Scott Clasen, John Calvin Schmidt
  • Publication number: 20040126784
    Abstract: The present invention relates to regulation of cellular proliferation. More particularly, the present invention is directed to nucleic acids encoding protein kinase C &zgr; (PKC-&zgr;), phospholipase C-&bgr;1 (PLC-&bgr;1), protein tyrosine kinase 2 (FAK), protein tyrosine kinase 2b (FAK2), casein kinase 2 (CK2), cMET tyrosine kinase (cMET), flap structure specific endonuclease 1 (FEN1), REV1 dCMP transferase (REV1), apurinic/apyrimidinic nuclease 1 (APE1), cyclin dependent kinase 3 (CDK3), PIM1 kinase (PIM1), cell division cycle 7 kinase (CDC7L1), cyclin dependent kinase 7 (CDK7), cytokine inducible kinase (CNK), potentially prenylated protein tyrosine phosphatase (PRL-3), serine threonine kinase 2 (STK2) or (NEK4), cyclin dependent serine threonine kinase (NKIAMRE), or histone acetylase (HBO1), which are involved in modulation of cell cycle arrest.
    Type: Application
    Filed: July 14, 2003
    Publication date: July 1, 2004
    Applicant: Rigel Pharmaceuticals, Incorporated
    Inventors: Yasumichi Hitoshi, Yonchu Jenkins, Vadim Markovtsov
  • Publication number: 20040126785
    Abstract: Colon cancer specific promoter sequences and adenovirus vehicles are provided. By providing for transcriptional initiating regulation dependent upon transcription factors that are only active in specific, limited cell types, virus replication will be restricted to the target cells. The modified adenovirus may be used as a vehicle for introducing new genetic capability, particularly associated with cytotoxicity for treating neoplasia.
    Type: Application
    Filed: July 21, 2003
    Publication date: July 1, 2004
    Inventors: Yuanhao Li, De-Chao Yu
  • Publication number: 20040126786
    Abstract: The present invention provides a method of screening subjects for disease risk. The method comprises determining the serotonin transporter gene promoter genotype of a subject. The serotonin transporter gene promoter genotype is used to indicate whether or not the subject is at increased risk of disease. The method is particularly adapted to screening for risk of disease in response to stress, and accordingly can be used to indicate interventions that manage stress, and hence reduce disease risk, in susceptible or higher-risk individuals.
    Type: Application
    Filed: July 23, 2003
    Publication date: July 1, 2004
    Inventor: Redford B. Williams
  • Publication number: 20040126787
    Abstract: The present invention discloses four groups of SCN5A variants that represent the most common SCN5A variants in humans. A specific mutation in one of the variants has been shown to display a different phenotype in relation to a human heart disease than other variants and known human sodium channel &agr; subunits with corresponding mutations. The present invention provides new tools to study mutations and to design or identify new diagnostic and treatment strategies or agents for sodium channel related diseases or conditions.
    Type: Application
    Filed: August 1, 2003
    Publication date: July 1, 2004
    Inventors: Jonathan C. Makielski, Bin Ye, Michael J. Ackerman
  • Publication number: 20040126788
    Abstract: Disclosed are methods for the use of Fibulin-5 as a marker for cancer diagnostics and other cancer screening assays, including to monitor the treatment of a patient with cancer, and for the use of Fibulin-5 as a cancer therapeutic and/or anti-angiogenesis therapeutic. Also disclosed are methods for identifying regulators of TGF&bgr; activity and methods for identifying regulators of tumorigenicity and angiogenesis.
    Type: Application
    Filed: August 13, 2003
    Publication date: July 1, 2004
    Inventors: William P. Schiemann, Harvey F. Lodish
  • Publication number: 20040126789
    Abstract: Disclosed are compositions preferably for use in nucleic acid synthesis that include one or more anti-reverse transcriptase (RT) antibodies and/or one or more anti-DNA polymerase (DNAP) antibodies and/or single strand binding proteins (SSBs). Some of the disclosed compositions include one or more anti-DNAP antibodies and/or one or more anti-RT antibodies and one or more SSBs. Other disclosed compositions include two or more SSBs. The disclosed nucleic acid synthesis compositions also can include one or more DNAPs, one or more RTs, one or more nucleotides, one or more primers, and/or one or more templates.
    Type: Application
    Filed: September 5, 2003
    Publication date: July 1, 2004
    Applicant: Invitrogen Corporation
    Inventors: Kyusung Park, Jun E. Lee
  • Publication number: 20040126790
    Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the release of labeled or unlabeled nucleotides that are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides released from a nucleic acid by exonuclease treatment are covalently cross-linked to nanoparticles and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus for nucleic acid sequencing.
    Type: Application
    Filed: September 12, 2003
    Publication date: July 1, 2004
    Inventors: Xing Su, Andrew A. Berlin, Selena Chan, Steven J. Kirch, Tae-Woong Koo, Gabi Neubauer, Valluri Rao, Narayanan Sundararajan, Mineo Yamakawa
  • Publication number: 20040126791
    Abstract: The present invention relates to a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of a cellular FLICE-like inhibitory protein (cFLIP) gene, comprising a complementary RNA strand which is substantially identical to at least a part of a cFLIP gene. The invention also relates to a pharmaceutical composition comprising the dsRNA together with a pharmaceutically acceptable carrier; methods for inhibiting the expression of a cFLIP gene in a cell, methods for improving the effectiveness of an apoptosis-inducing drug, and methods for treating cancer using the pharmaceutical composition.
    Type: Application
    Filed: September 19, 2003
    Publication date: July 1, 2004
    Applicant: Ribopharma AG
    Inventors: Harald Wajant, Klaus Pfizenmaier, Stefan Limmer, Roland Kreutzer, Hans-Peter Vornlocher
  • Publication number: 20040126792
    Abstract: The present invention relates to the synthesis of novel compounds for the inhibition of peptide synthesis in the ribosome. It also relates to improved methods for directly monitoring peptide bond formation in the ribosome. These compounds can be used for high throughput screening of proposed therapeutically active drug species (e.g., antibiotics) targeted to ribosomes.
    Type: Application
    Filed: September 17, 2003
    Publication date: July 1, 2004
    Inventor: Biliang Zhang
  • Publication number: 20040126793
    Abstract: The present invention provides a fusion polypeptide which can bind to a cell surface binding moiety (e.g., a carbohydrate) and serve as a ligand for a cell surface polypeptide, as well as a vector comprising a nucleic acid encoding for such a fusion polypeptide, and a host cell comprising such nucleic acid. The present invention also provides a composition comprising an antigen bearing target and such a fusion polypeptide, as well as a composition comprising a virus or a cell and such a fusion polypeptide. The present invention further relates to a method of modulating an immune response in an animal using such compositions.
    Type: Application
    Filed: September 19, 2003
    Publication date: July 1, 2004
    Applicant: Genitrix, LLC
    Inventors: Andrew H. Segal, Elihu Young
  • Publication number: 20040126794
    Abstract: The present invention provides methods and reagents for detecting an individual's increased or decreased risk for type 1 diabetes, also known as, insulin dependent diabetes mellitus (“IDDM”).
    Type: Application
    Filed: September 25, 2003
    Publication date: July 1, 2004
    Inventors: Teodorica L. Bugawan, Henry A. Erlich
  • Publication number: 20040126795
    Abstract: A method for screening for scrotal hernias in animals is disclosed. The method involves assays for genetic differences in the MIS, GPX4A, and FSHb genes of the animal which are associated with scrotal hernias.
    Type: Application
    Filed: October 3, 2003
    Publication date: July 1, 2004
    Inventors: Tun-Ping Yu, Nicholas G. Hopkins, Shoji Sasaki, Lizhen Wang, Johannes W. M. Bastiaansen, Nader Deeb, Eldon R. Wilson, Alan J. Mileham
  • Publication number: 20040126796
    Abstract: Methods and compositions are provided for extracting DNA from tissues, hair, teeth, bone, plant material, solid matrices and other samples from which DNA extraction is generally regarded as being difficult. DNA may quickly be extracted from samples, for example, in just 5 minutes to 2 hours, without enzymatic digestion or the use of toxic organic chemicals such as phenol, chloroform, guanidine thiocyanate or 2-mercaptoethanol.
    Type: Application
    Filed: October 7, 2003
    Publication date: July 1, 2004
    Inventors: David Carlson, James G. Lazar, Michael Connolly
  • Publication number: 20040126797
    Abstract: The present invention relates to a micro-array system and the uses thereof. Particularly, the micro-array system is for a micro amount of biomolecules carrying on a bioreaction in a reaction solution, which comprises a substrate comprising a plurality of micro-wells for receiving the reaction solution; a plurality of micro-beads placing in the reaction solution for the biomolecules attached on surfaces thereon; and a vibrating module for vibrating the substrate, which makes the biomolecules attached on the micro-beads react evenly. Optionally, the micro-array system further comprises a temperature control module for controlling the temperature of the reaction solution. The bioreaction performed in the micro-array system according to the invention has the advantages of having a high sensitivity, being easily post-manipulated, being easily operated, having a high reliability and being reusable.
    Type: Application
    Filed: October 21, 2003
    Publication date: July 1, 2004
    Applicants: NATIONAL TSING-HUA UNIVERSITY, STANFORD UNIVERSITY
    Inventors: Chih-Ying Hsu, Tzong-Hsiung Hseu, Ruey-Shing Huang, David R. Cox, Chien-ming Wu, Jiann Heng Chen, Min-Lung Tsai, Ming-Tsong Lai, Yong-Li Pan, Tai-ching Fan
  • Publication number: 20040126798
    Abstract: Methods are provided for detecting the sense and antisense transcripts and for determining template strand of a genomic DNA. Exemplary methods include reverse transcribing transcripts without second strand synthesis. The resulting single stranded stranded DNA is labeled and detected using nucleic acid probe arrays. In a particularly preferred embodiment, actinomycin is used to inhibit the synthesis of second strand cDNA during reverse transcription.
    Type: Application
    Filed: December 5, 2003
    Publication date: July 1, 2004
    Applicant: Affymetrix, INC.
    Inventors: Carsten Rosenow, Rini Saxena
  • Publication number: 20040126799
    Abstract: The present invention relates to new polynucleotides derived from the nucleotide sequence of the IFN&agr;-7 gene comprising new SNPs, and new polypeptides derived from the wild-type IFN&agr;-7 protein comprising at least one mutation caused by at least one SNP of the invention as well as their therapeutic uses.
    Type: Application
    Filed: December 11, 2003
    Publication date: July 1, 2004
    Inventor: Jean-Louis Escary
  • Publication number: 20040126800
    Abstract: Polynucleotides containing single nucleotide polymorphisms which are present in transcription factor binding site clusters are disclosed. The polynucleotides can be used in diagnostic assays, in testing systems for finding new drugs, and in methods for treating diseases. Also disclosed are methods for identifying single nucleotide polymorphisms which are present in transcription factor binding site clusters.
    Type: Application
    Filed: December 11, 2003
    Publication date: July 1, 2004
    Inventor: Volker Nowotny
  • Publication number: 20040126801
    Abstract: The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating diseases, disorders, and/or conditions related to these novel human secreted proteins.
    Type: Application
    Filed: December 12, 2003
    Publication date: July 1, 2004
    Applicant: Human Genome Sciences, Inc.
    Inventors: Michele Fiscella, Ping Wei, David W. LaFleur, Henrik S. Olsen, Kevin P. Baker, Reinhard Ebner, George A. Komatsoulis, Craig A. Rosen, Steven M. Ruben, D. Roxanne Duan, Paul E. Young, Kimberly A. Florence, Paul A. Moore, Charles E. Birse, Jian Ni, Daniel R. Soppet, Yanggu Shi
  • Publication number: 20040126802
    Abstract: In one aspect of the invention, a nanotube based microarray is provided. The nanotube microarray contains nanotubes connecting with electrodes for detecting the electrical characteristics of nanotubes.
    Type: Application
    Filed: December 12, 2003
    Publication date: July 1, 2004
    Applicant: Affymetrix, INC.
    Inventor: Shane Brubaker
  • Publication number: 20040126803
    Abstract: A method for profiling and identifying persons by using data samples provides a collapsed list of one or more aggregated matching samples having consistent STR profiles. Each of the one or more aggregated matching samples are presented on a one-line display, the one-line display having a composite profile representing consensus of all STR profiles in an associated aggregate matching sample. The one-line display of an aggregated matching sample may be expanded, and the expanded one-line display provides a view of all member samples in the aggregated matching sample. A method for aggregating samples from a plurality of disparate samples, and combining the aggregated samples is also provided.
    Type: Application
    Filed: December 15, 2003
    Publication date: July 1, 2004
    Inventors: Howard Cash, Simon Mercer, Jonathon Hoyle, Lucy Hadden, Amy Sutton, David Relyea, William Wake, Matthew Smith, Tom Kubit, Greg Poth, Anna Korn, Anna Khizhnyak, Tracy Beeson
  • Publication number: 20040126804
    Abstract: A BLAST analysis unit calculates the homologies between data included in an analysis target data group and the first data group, respectively, and calculates the number of data exhibiting homologies (orthologous gene count) as a first homology value x by using the calculated values. This unit sets nA thresholds E and calculates a first homology value xi for each threshold Ei, and calculates the homologies between data included in the analysis target data group and the second data group, respectively. The unit calculates the number of data exhibiting homologies (orthologous gene count) as a second homology value y. The unit sets nB thresholds E and calculates a second homology value yi for each threshold Ei. A t-test unit determines to which one of the first and second data groups the analysis target data group is similar.
    Type: Application
    Filed: December 17, 2003
    Publication date: July 1, 2004
    Inventors: Takao Shinozawa, Tokumasa Horiike, Kazuo Hamada, Shigehiko Kanaya
  • Publication number: 20040126805
    Abstract: The invention provides a human Nek1-related protein kinase (NRPK) and polynucleotides which identify and encode NRPK. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating or preventing disorders associated with expression of NRPK.
    Type: Application
    Filed: December 19, 2003
    Publication date: July 1, 2004
    Applicant: Incyte Corporation
    Inventors: Olga Bandman, Neil C. Corley, Karl J. Guegler, Mariah R. Baughn
  • Publication number: 20040126806
    Abstract: A method is described for predetermining ratios of primer pairs present in a single reaction vessel so as to achieve approximately equimolar yield of products. The ratios are determined as a function of the length of the amplicon and the length of other amplicons being simultaneously tested. The primers may desirably be for p53 gene sequences.
    Type: Application
    Filed: December 23, 2003
    Publication date: July 1, 2004
    Applicant: AFFYMETRIX, INC.
    Inventors: Hajime Matsuzaki, Eric A. Murphy
  • Publication number: 20040126807
    Abstract: The present invention is directed to compositions of matter useful for the diagnosis and treatment of tumor in mammals and to methods of using those compositions of matter for the same.
    Type: Application
    Filed: January 15, 2004
    Publication date: July 1, 2004
    Applicant: Genentech, Inc.
    Inventors: Audrey Goddard, Paul J. Godowski, Austin L. Gurney, Kenneth J. Hillan, Paul Polakis, Victoria Smith, William I. Wood, Thomas D. Wu, Zemin Zhang
  • Publication number: 20040126808
    Abstract: Cancer associated antigens have been identified by autologous antibody screening of libraries of nucleic acids expressed in small cell lung cancer cells using antisera from cancer patients. The invention relates to nucleic acids and encoded polypeptides which are cancer associated antigens expressed in patients afflicted with small cell lung cancer. The invention provides, inter alia, isolated nucleic acid molecules, expression vectors containing those molecules and host cells transfected with those molecules. The invention also provides isolated proteins and peptides, antibodies to those proteins and peptides and cytotoxic T lymphocytes which recognize the proteins and peptides. Fragments of the foregoing including functional fragments and variants also are provided. Kits containing the foregoing molecules additionally are provided.
    Type: Application
    Filed: January 20, 2004
    Publication date: July 1, 2004
    Inventors: Ali O. Gure, Elisabeth Stockert, Matthew J. Scanlan, Dirk Jager, Lloyd J. Old, Yao-Tseng Chen
  • Publication number: 20040126809
    Abstract: Hepatitis C virus helicase has been crystallized as tetragonal and orthorhombic crystals, and the structures of the crystals has been solved. The structure coordinates of the crystal structures are useful for solving the structures of other molecules or molecular complexes.
    Type: Application
    Filed: May 2, 2001
    Publication date: July 1, 2004
    Inventors: Barry C. Finzel, Melissa S. Harris, Eric T. Baldwin
  • Publication number: 20040126810
    Abstract: The invention concerns a peptide substrate identified by type BoNT/B botulinus toxin, characterised in that it incorporates in its structure a fragment of formula PYA-(Z)-pNF wherein Z represents one or several amino acids, said fragment being cleavable by said toxin.
    Type: Application
    Filed: September 12, 2003
    Publication date: July 1, 2004
    Inventors: Bernard Roques, Christine Anne
  • Publication number: 20040126811
    Abstract: An isolated Helicobacter pylori protein binding to sialyl-Lewis x antigen and having an approximate molecular weight of 66 kDa and comprising the amino acid sequences SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, and sialyl-Lewis x antigen-binding H. pylori alleles of the protein, recombinant forms of the protein, such as a protein having the amino acid sequence SEQ ID NO: 5, or the protein alleles, and sialyl-Lewis x antigen binding portions of the proteins, are disclosed. The protein or portion of protein maybe used as a medicament or diagnostic antigen, and can be used in a method of determining the presence of sialyl-Lewis x antigen-binding H. pylori bacteria in a biological sample. Further, a DNA molecule encoding the protein or portion of protein, a vector comprising the DNA molecule, and a host transformed with the vector are comprised by the disclosure. Additionally, a method of determining the presence of sialyl-Lewis x or related carbohydrate structures in a sample, is described.
    Type: Application
    Filed: December 24, 2003
    Publication date: July 1, 2004
    Inventors: Thomas Boren, Lennart Hammarstrom
  • Publication number: 20040126812
    Abstract: The invention describes a method for isolating an intestinal protein which is able to bind cholesterol and/or cholesterol uptake inhibitors. Methods of identifying inhibitors of cholesterol uptake and related pharmaceuticals and methods of treatment also are described.
    Type: Application
    Filed: August 6, 2003
    Publication date: July 1, 2004
    Inventors: Werner Kramer, Wendelin Frick
  • Publication number: 20040126813
    Abstract: The present invention relates to methods and systems for sorting a plurality of protein structures generated by a database search based upon their corresponding sequences. The methods and systems according to the invention are based upon computer generated graphical user interfaces that present to the user: i) a multiple sequence alignment of the sequences corresponding to the structures to be sorted; ii) the means to identify whether one or more of the sequences comprise homologous alignment domains; and iii) the means to select one or more sequences for subsequent processing of their corresponding structures. In another aspect of the invention, the user is presented one or more phylogenetic tree representations of user selected homologous alignment domains so that the user may quickly determine the evolutionary similarity of a family of homologous alignment domains.
    Type: Application
    Filed: August 29, 2003
    Publication date: July 1, 2004
    Inventors: Derek A. Debe, Jeffrey Regan, Joseph F. Danzer
  • Publication number: 20040126814
    Abstract: A sensor for detecting a target analyte using a molecularly imprinted polymer (MIP) that has been imprinted with the target analyte. The MIP may be used as a working electrode in electrochemical impedance spectroscopy by either coating a substrate or being pressed into a disk.
    Type: Application
    Filed: September 16, 2003
    Publication date: July 1, 2004
    Inventors: Waheguru Pal Singh, Anthony Giletto, Jinseong Kim, Craig Andrews, Jerome Brown, Brian Watkins, Anuncia Gonzalez-Martin
  • Publication number: 20040126815
    Abstract: The invention is a process for purifying TAFIa from a serum sample in the presence of a reversible inhibitor of TAFIa. The invention is also a method of measuring the activity of TAFIa in the presence of a reversible inhibitor.
    Type: Application
    Filed: December 16, 2003
    Publication date: July 1, 2004
    Inventors: Shi-Shan Mao, Dennis Colussi, Steve Gardell