Patents Issued in March 4, 2014
  • Patent number: 8663890
    Abstract: Provided is an electrostatic charge image developing toner, each toner particle including a core portion, a first shell layer, and a second shell layer, wherein the core portion contains a first polyester resin, a colorant, and a release agent, the first shell layer contains a second polyester resin and covers the core portion, the second shell layer contains a polymer of an aromatic vinyl monomer and a third polyester resin having an ethylenic unsaturated double bond that is polymerizable with the aromatic vinyl monomer, and covers the first shell layer, and a total amount of the first shell layer and the second shell layer is within a range of from 16% by weight to 40% by weight of the toner particle.
    Type: Grant
    Filed: July 3, 2012
    Date of Patent: March 4, 2014
    Assignee: Fuji Xerox Co., Ltd.
    Inventors: Shinpei Takagi, Soichiro Kitagawa, Shinya Sakamoto, Tomohiro Shinya
  • Patent number: 8663891
    Abstract: Disclosed are a toner for developing an electrostatic latent image and a method of preparing the same. The toner may include latex, a pigment and a releasing agent. The lowest crossover temperature of the toner at which the storage modulus (G?) and the loss modulus (G?) of the toner are substantially equal to each other may be in the range of about 65 to about 80° C. The weight average molecular weight (Mw) of the toner may be in the range of about 65,000 to about 75,000. The z-average molecular weight (Mz) of the toner may be in the range of about 110,000 to about 220,000.
    Type: Grant
    Filed: December 7, 2009
    Date of Patent: March 4, 2014
    Assignee: SAMSUNG Electronics Co., Ltd.
    Inventors: Jun-Young Lee, Min-Young Cheong, Jae-Hwan Kim, Yo-Da Shin, Tae-Hoe Koo
  • Patent number: 8663892
    Abstract: A latent electrostatic image developing carrier including: core particles each having magnetism; and a coating layer covering each of the core particles, wherein the latent electrostatic image developing carrier has a shape factor SF-2 of 115 to 150 and has a bulk density of 1.8 g/cm3 to 2.4 g/cm3, wherein the core particles have a shape factor SF-2 of 120 to 160 and have an arithmetic mean surface roughness Ra of 0.5 ?m to 1.0 ?m, and wherein the coating layer contains a resin and a filler and an amount of the filler contained in the coating layer is 50 parts by mass to 500 parts by mass per 100 parts by mass of the resin contained in the coating layer.
    Type: Grant
    Filed: August 23, 2012
    Date of Patent: March 4, 2014
    Assignee: Ricoh Company, Ltd.
    Inventors: Shigenori Yaguchi, Kimitoshi Yamaguchi, Toyoshi Sawada, Hitoshi Iwatsuki, Toyoaki Tano, Hiroshi Tohmatsu
  • Patent number: 8663893
    Abstract: To provide a fixing solution for fixing fine resin particles to a recording medium, the fixing solution including: a diluent which contains water; a foaming agent which allows the fixing solution to be in the form of foam; and plasticizers which soften or swell at least part of the fine resin particles, wherein the plasticizers contain a liquid plasticizer which is liquid at normal temperature and soluble in the diluent, and a solid plasticizer which is solid at normal temperature and soluble in the diluent.
    Type: Grant
    Filed: July 1, 2010
    Date of Patent: March 4, 2014
    Assignee: Ricoh Company, Ltd.
    Inventors: Yasuo Katano, Tsuneo Kurotori, Yukimichi Someya, Fuminari Kaneko, Yuko Arizumi, Shigeo Takeuchi, Shigenobu Hirano, Hidenori Tomono, Hidekazu Yaginuma
  • Patent number: 8663894
    Abstract: Method of adjusting the melt flow index of a toner is described, the method including adjusting the pH of the toner after the toner particles have been coalesced and the adjusting alters the melt flow index of the toner.
    Type: Grant
    Filed: August 29, 2012
    Date of Patent: March 4, 2014
    Assignee: Xerox Corporation
    Inventor: Daniel W. Asarese
  • Patent number: 8663895
    Abstract: According to one embodiment, a method for manufacturing a template for imprinting includes preparing a first template having a device pattern and a plurality of identification patterns, and forming a second template by transferring the device pattern and at lest desired one of the identification patterns to a template substrate.
    Type: Grant
    Filed: September 16, 2010
    Date of Patent: March 4, 2014
    Assignee: Kabushiki Kaisha Toshiba
    Inventor: Yoshihito Kobayashi
  • Patent number: 8663896
    Abstract: A resin composition for laser engraving is provided that includes (Component A) a compound having a hydrolyzable silyl group and/or a silanol group, (Component B) a depolymerization catalyst and/or a depolymerization catalyst precursor, and (Component C) a depolymerizable binder polymer.
    Type: Grant
    Filed: May 6, 2011
    Date of Patent: March 4, 2014
    Assignee: FUJIFILM Corporation
    Inventor: Atsushi Sugasaki
  • Patent number: 8663897
    Abstract: According to the present invention, there is provided a polymerizable fluorine-containing sulfonic acid onium salt of the following general formula (2) and a resin obtained by polymerization thereof. It is possible by the use of this sulfonate resin of the present invention to provide a resist composition with high resolution, board depth of focus tolerance (DOF), small line edge roughness (LER) and high sensitivity. In the formula, Z represents a substituted or unsubstituted C1-C6 straight or branched alkylene group, or a divalent moiety in which substituted or unsubstituted C1-C6 straight or branched alkylene groups are bonded in series to a divalent group obtained by elimination of two hydrogen atoms from an alicyclic or aromatic hydrocarbon; R represents a hydrogen atom, a halogen atom, or a C1-C3 alkyl or fluorine-containing alkyl group; and Q+ represents a sulfonium cation or an iodonium cation.
    Type: Grant
    Filed: October 8, 2009
    Date of Patent: March 4, 2014
    Assignee: Central Glass Company, Limited
    Inventors: Takashi Masubuchi, Kazunori Mori, Yuji Hagiwara, Satoru Narizuka, Kazuhiko Maeda
  • Patent number: 8663898
    Abstract: There is disclosed A resist underlayer film composition, wherein the composition contains a polymer obtained by condensation of, at least, one or more compounds represented by the following general formulae (1-1) and/or (1-2), one or more kinds of a compound represented by the following general formula (2), and one or more kinds of a compound, represented by the following general formula (3), and/or an equivalent body thereof. There can be provided an underlayer film composition, especially for a trilayer resist process, that can form an underlayer film having reduced reflectance, (namely, an underlayer film having optimum n-value and k-value as an antireflective film), excellent filling-up properties, high pattern-antibending properties, and not causing line fall or wiggling after etching especially in a high aspect line that is thinner than 60 nm, and a patterning process using the same.
    Type: Grant
    Filed: December 5, 2011
    Date of Patent: March 4, 2014
    Assignee: Shin-Etsu Chemical Co., Ltd.
    Inventors: Tsutomu Ogihara, Takeru Watanabe, Yusuke Biyajima, Daisuke Kori, Takeshi Kinsho, Toshihiko Fujii
  • Patent number: 8663899
    Abstract: A resist composition having a resin having a structural unit represented by the formula (I), a resin being insoluble or poorly soluble in alkali aqueous solution, but becoming soluble in an alkali aqueous solution by the action of an acid and not including the structural unit represented by the formula (I), and an acid generator, wherein R1, A1, R2, Rb1, Rb2, Lb1, ring Wb1, Rb3, Rb4, and Z1+ are defined in the specification.
    Type: Grant
    Filed: July 18, 2012
    Date of Patent: March 4, 2014
    Assignee: Sumitomo Chemical Company, Limited
    Inventors: Koji Ichikawa, Takashi Hiraoka, Hiromu Sakamoto
  • Patent number: 8663900
    Abstract: A resist composition having a resin having a structural unit represented by the formula (I), a resin being insoluble or poorly soluble in alkali aqueous solution, but becoming soluble in an alkali aqueous solution by the action of an acid and not including the structural unit represented by the formula (I), and an acid generator represented by the formula (II), wherein R1, A1, A13, A14, X12, RII1, RII2, LII1, YII1, RII3, RII4, RII5, RII6, RII7, n, s and RII8 are defined in the specification.
    Type: Grant
    Filed: July 18, 2012
    Date of Patent: March 4, 2014
    Assignee: Sumitomo Chemical Company, Limited
    Inventors: Koji Ichikawa, Yukako Anryu, Shingo Fujita
  • Patent number: 8663901
    Abstract: The present invention relates to a fluorine-free photoacid generator (PAG) and a photoresist composition containing the same. The PAG is characterized by the presence of an onium cationic component and a fluorine-free fused ring heteroaromatic sulfonate anionic component containing one or more electron withdrawing substituents. The onium cationic component of the PAG is preferably a sulfonium or an iodonium cation. The photoresist composition further contains an acid sensitive imaging polymer. The photoresist composition is especially useful for forming material patterns on a semiconductor substrate using 193 nm (ArF) lithography.
    Type: Grant
    Filed: September 13, 2012
    Date of Patent: March 4, 2014
    Assignee: International Business Machines Corporation
    Inventors: Sen Liu, Pushkara R. Varanasi
  • Patent number: 8663902
    Abstract: A method of forming an image on a substrate, which comprises applying to the substrate an activatable color forming compound wherein said activatable color forming compound is initially unreactive but becomes reactive upon activation; activating said color forming compound in the areas of the substrate where the image is to be formed, and, reacting the activated color forming compound into its colored form to produce an image. A substrate imaged using this method is also provided.
    Type: Grant
    Filed: April 1, 2010
    Date of Patent: March 4, 2014
    Assignee: Datalase Ltd
    Inventors: Anthony Jarvis, Martin Walker, Christopher Wyres, Tristan Phillips
  • Patent number: 8663903
    Abstract: Disclosed is a top coating composition formed on a resist film, for protecting the resist film, the top coating composition being a top coating composition for photoresist, characterized by containing a fluorine-containing polymer having a repeating unit represented by the following general formula (1). This composition is capable of controlling developing solution solubility and has a high water repellency. [In the formula, R1 represents a hydrogen atom, fluorine atom, methyl group or trifluoromethyl group, R2 represents a heat-labile protecting group, R3 represents a fluorine atom or fluorine-containing alkyl group, and W is a bivalent linking group.
    Type: Grant
    Filed: April 20, 2010
    Date of Patent: March 4, 2014
    Assignee: Central Glass Company, Limited
    Inventors: Kazuhiko Maeda, Takamasa Kitamoto, Haruhiko Komoriya, Satoru Narizuka, Yoshimi Isono, Kazunori Mori
  • Patent number: 8663904
    Abstract: Embodiments in accordance with the present invention provide for non-self imageable norbornene-type polymers useful for immersion lithographic processes, methods of making such polymers, compositions employing such polymers and immersion lithographic processes that make use of such compositions. More specifically the embodiments of the present invention are related to norbornene-type polymers useful for forming top-coat layers for overlying photoresist layers in immersion lithographic process and the process thereof.
    Type: Grant
    Filed: August 7, 2013
    Date of Patent: March 4, 2014
    Assignee: Promerus, LLC
    Inventors: Pramod Kandanarachchi, Kazuyoshi Fujita, Steven Smith, Larry F Rhodes
  • Patent number: 8663905
    Abstract: A resist under layer film-forming composition comprises (A) an aminated fullerene having at least one amino group bonded to a fullerene skeleton, and (B) a solvent. The composition exhibits excellent etching resistance, causes an under layer film pattern to bend only with difficulty in a dry etching process, and can transfer a resist pattern faithfully onto a substrate to be processed with high reproducibility.
    Type: Grant
    Filed: November 19, 2007
    Date of Patent: March 4, 2014
    Assignee: JSR Corporation
    Inventors: Nakaatsu Yoshimura, Yousuke Konno
  • Patent number: 8663906
    Abstract: The present invention provides a composition for forming a fine pattern with high dry etching resistance and a method for forming the fine pattern. The composition for fine pattern formation containing: a resin containing a repeating unit having a silazane bond; and a solvent as well as a method for fine pattern formation using the same.
    Type: Grant
    Filed: September 12, 2008
    Date of Patent: March 4, 2014
    Assignee: AZ Electronic Materials USA Corp.
    Inventors: Ralph R Dammel, Wen-Bing Kang, Yasuo Shimizu, Tomonori Ishikawa
  • Patent number: 8663907
    Abstract: Provided is a method of forming pattern including (a) forming a chemically amplified resist composition into a film, (b) exposing the film to light, and (c) developing the exposed film with a developer containing a first organic solvent, wherein in the developer, particles each having a diameter of 0.3 ?m or greater amount to a density of 30 particles/ml or less.
    Type: Grant
    Filed: August 24, 2011
    Date of Patent: March 4, 2014
    Assignee: FUJIFILM Corporation
    Inventors: Keita Kato, Sou Kamimura, Yuichiro Enomoto, Kaoru Iwato, Shohei Kataoka, Shoichi Saitoh
  • Patent number: 8663908
    Abstract: Provided are a method of conveniently and efficiently removing abnormal prion protein from a blood product and a method of removing leukocyte simultaneously with abnormal prion protein. The method of removing an abnormal prion protein from a blood product includes filtering the blood product through a filter filled with a carrier coated with a polymer, which is composed of three units including 20 mol % or more and 40 mol % or less of a unit originating from a hydrophobic polymerizable monomer, 5 mol % or more and 13 mol % or less of a unit originating from a polymerizable monomer containing a basic nitrogen-containing part, and a unit originating from a polymerizable monomer containing a protonic neutral hydrophilic part as the balance, and then recovering the filtered blood product.
    Type: Grant
    Filed: July 12, 2007
    Date of Patent: March 4, 2014
    Assignee: Asahi Kasei Medical Co., Ltd.
    Inventors: Hiromi Nirasawa, Morikazu Miura
  • Patent number: 8663909
    Abstract: This invention describes a device consisting of a micro channel plate, filter, and porous holder for filter, which is substituted by a pure agar block during method performance, and supportive structural elements. The device is intended for rapid detection and/or identification of microorganisms. Microorganisms are trapped by filtration in long (diameter/length=1/10-1/100), cylindrical, parallel, micro-channels that are open from both sides and attached to a filter from one side. A micro channel plate houses a multiplicity of micro channels (possible diameter of each channel=1-30 ?m, length 100-1000 ?m, and number on centimeter2=100,000-1,000,000). The micro channel plate with cells trapped on the surface of the filter is attached to an agar block impregnated by artificial substrate(s) so that the molecules of the artificial substrates will fill all micro channels. Trapped cells produce colored or fluorescent molecules from artificial substrates.
    Type: Grant
    Filed: April 20, 2005
    Date of Patent: March 4, 2014
    Assignee: Nanologix, Inc.
    Inventor: Sergey Gazenko
  • Patent number: 8663910
    Abstract: A plurality of kinds of liquids, which are of at least three kinds, containing (a) a test body solution containing at least one kind of an analyte, and (b) at least two kinds of liquids selected from the group consisting of a reagent solution, an amplifying solution, and a detecting solution, are fed to a detection site containing a specific binding substance with respect to the analyte. A qualitative analysis or a quantitative analysis of the analyte contained in the test body solution is thereby performed. Directions of liquid feeding of all of the plurality of the kinds of the liquids vary from one another, and the plurality of the kinds of the liquids are caused to intersect with one another at the detection site.
    Type: Grant
    Filed: September 18, 2009
    Date of Patent: March 4, 2014
    Assignee: FUJIFILM Corporation
    Inventors: Mikinaga Mori, Junichi Katada, Hiroyuki Chiku, Takayoshi Oyamada
  • Patent number: 8663911
    Abstract: Methods, assays, and apparatus are disclosed for testing of antigens associated with intestinal and/or blood-brain barrier permeability. For example, blood, saliva or other bodily fluid can be tested for binding (1) to a bacterial toxin (preferably a lipopolysaccharide), and (2) binding to tissue antigens selected from at least one of (a) a gut-related antigen and (b) a blood brain barrier-related antigen. Analysis of test results can be used to assist in detecting and diagnosing diseases associated with leaky gut syndrome (whether due to paracellular or transcellular pathways, and whether due to bacterial toxins or some other cause) and/or to diseases associated with excessive blood brain barrier permeability, which are contemplated herein to include both neuroinflammation and/or neuroautoimmunity conditions, and especially amyotrophic lateral sclerosis, Parkinsons disease, multiple sclerosis, Alzheimer's, or peripheral neuropathy, and major depression.
    Type: Grant
    Filed: January 26, 2012
    Date of Patent: March 4, 2014
    Assignees: Cyrex Laboratories, LLC, Immunosciences Lab, Inc.
    Inventor: Aristo Vojdani
  • Patent number: 8663912
    Abstract: Disclosed are fluorogenic substrates for measuring ADAMTS13 activity or ADAMTS13 inhibitor activity. Substrates can comprise an oligopeptide which can consist of up to 80 amino acids of sequence of von Willebrand Factor (VWF). The oligopeptide can include modifications of sequence of VWF, including an amino-terminal glycine, a scissile Y-M peptide, and a cysteine substitution located from 1 to 12 amino acids from the scissile Y-M in the carboxy terminal direction. A substrate can further comprise a fluorophore and a fluorescence quencher bound to the oligopeptide on opposite sides of the scissile Y-M peptide, wherein the fluorescence quencher is not identical to the fluorophore. An oligopeptide can be encoded by a nucleic acid sequence which can also encode a His tag. An oligopeptide can be expressed in a cell or microorganism. Also disclosed are methods of using a fluorogenic substrate to measure ADAMTS13 activity or ADAMTS13 inhibitor activity.
    Type: Grant
    Filed: July 18, 2012
    Date of Patent: March 4, 2014
    Assignee: Washington University
    Inventors: J. Evan Sadler, Joshua Muia, Weiqiang Gao
  • Patent number: 8663913
    Abstract: Bacteriophages in general and lambda phage in particular are powerful, flexible reagents who have yet to be exploited to their full potential. As discussed herein, the lambda phage head and/or genome comprises an easy to use and highly efficient delivery vehicle for delivering the expression products of a gene of interest systemically or to a particular tissue.
    Type: Grant
    Filed: May 11, 2009
    Date of Patent: March 4, 2014
    Inventor: Sidney Hayes
  • Patent number: 8663914
    Abstract: A system and method for treating bacterial cells with an electrochemical process, alone or in combination with antibiotics. Weak electric currents are used to effectively eliminate bacterial cells. The method may be adapted for novel therapies of chronic infections and strategies to control persistent biofouling. The system has broad spectrum applications in treating chronic and drug resistant infections, such as those caused by Pseudomonas aeruginosa, Mycobacterium tuberculosis and methicillin resistant Staphylococcus aureus, and may also be used for decontamination of medical devices.
    Type: Grant
    Filed: February 18, 2011
    Date of Patent: March 4, 2014
    Assignee: Syracuse University
    Inventors: Dacheng Ren, Mi Zhang, Tagbo Niepa, Jeremy Gilbert
  • Patent number: 8663915
    Abstract: The present invention relates to methods of controlling tumorigenesis, including preventing, inhibiting, arresting or reversing tumorigenesis. The present invention also provides methods of treatment, prevention and diagnosis of neoblastoma, including cancer, such as metastatic cancer. In one method one or more cells having a predisposition to turn tumorigenic are identifyied. In the one or more cells an altered amount, an altered subcellular localisation or an altered activity of a Rab binding protein is detected. In a method of diagnosing the risk of developing a neoplasmt at least one of the expression level, the activity level and the subcellular localisation of a Rab binding protein is determined. In an in-vitro method of identifying a compound capable of forming a complex with a Rab binding protein the components that form the respective complex are contacted with each other.
    Type: Grant
    Filed: April 11, 2008
    Date of Patent: March 4, 2014
    Assignee: Agency for Science, Technology and Research
    Inventors: Lance D. Miller, Bing Lim, Jinqiu Zhang
  • Patent number: 8663916
    Abstract: The present invention is directed to methods of using nucleic acid molecules encoding for fusion peptides to produce the fusion peptides. The methods can include preparing or providing the nucleic acid molecules that having a fungal targeting agent (e.g., a fungal pheromone, such as alpha-mating pheromone) and a channel-forming domain consisting essentially of amino acids 451-626 of colicin Ia. The nucleic acid molecules can be transfected into host cells to produce the fusion peptide. The fusion peptides of the peptides of the present invention are particularly useful for the treatment of fungal infections in a wide variety of organisms.
    Type: Grant
    Filed: March 15, 2011
    Date of Patent: March 4, 2014
    Assignee: Pheromonicin Biotech, Ltd.
    Inventor: Xiao-Qing Qiu
  • Patent number: 8663917
    Abstract: It is an object of the present invention to provide a solution to problems associated with the use of microarray technology for the analysis DNA. The present invention provides compositions and methods for the use of simple and compound representations of DNA in microarray technology. The present invention is also directed to methods for the production of High Complexity Representations (HCRs) of the DNA from cells.
    Type: Grant
    Filed: March 30, 2005
    Date of Patent: March 4, 2014
    Assignee: Cold Spring Harbor Laboratory
    Inventors: Michael Wigler, Robert Lucito
  • Patent number: 8663918
    Abstract: A method for preparing a sample by utilizing a shearing force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range. Once nucleic acid molecules are obtained, magnetic entanglement particles are used to concentrate and clean the nucleic acid molecules for further testing.
    Type: Grant
    Filed: May 24, 2010
    Date of Patent: March 4, 2014
    Assignee: Integrated Nano-Technologies, Inc.
    Inventors: Dennis M. Connolly, Charles DeBoer, Vera Tannous, Christopher Kilcoin, Konstantin Aptekarev, David B. Bailey, Richard S. Murante
  • Patent number: 8663919
    Abstract: Disclosed herein are compositions, methods and kits for analyzing three-dimensional chromatin and/or chromosome conformation. Method are also disclosed for using the methods disclosed herein for diagnosing diseases such as cancer.
    Type: Grant
    Filed: May 18, 2012
    Date of Patent: March 4, 2014
    Assignee: Life Technologies Corporation
    Inventors: Shoulian Dong, Junko F. Stevens, Chunmei Liu, Cora L. Woo, Luz Montesclaros
  • Patent number: 8663920
    Abstract: Methods of characterizing a nucleic acid library by digital assay.
    Type: Grant
    Filed: July 30, 2012
    Date of Patent: March 4, 2014
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Serge Saxonov, Svilen S. Tzonev, Michael Y. Lucero, Ryan T. Koehler, Benjamin J. Hindson
  • Patent number: 8663921
    Abstract: The invention provides tandem single nucleotide polymorphisms and methods for their use, for example, in diagnosing Down Syndrome.
    Type: Grant
    Filed: August 4, 2010
    Date of Patent: March 4, 2014
    Assignee: University of Louisville Research Foundation, Inc.
    Inventors: Aoy Tomita Mitchell, Michael Mitchell
  • Patent number: 8663922
    Abstract: To minimize cross talk in systems and methods for detecting two or more different optical signals emitted from each of a plurality of reaction receptacles, an excitation signal associated with each of the optical signals has a known excitation frequency, and any detected signal having a frequency that is inconsistent with the excitation frequency is discarded. The receptacles are moved relative to optical sensors configured to detect each unique optical signal from an associated receptacle, and to further minimize cross talk, the optical sensors are arranged so that only one reaction receptacle at a time is in a signal detecting position with respect to one of its associated optical sensors, and the optical sensors are grouped by the optical signal they are configured to detect so that a first optical signal is detected from each of the reaction receptacles before a second optical signal is detected from the reaction receptacles.
    Type: Grant
    Filed: June 1, 2010
    Date of Patent: March 4, 2014
    Assignee: Gen-Probe Incorporated
    Inventors: Matthew J. Hayes, Byron J. Knight, Haitao Li, Robert F. Scalese
  • Patent number: 8663923
    Abstract: A nucleotide probe permitting the detection of nucleic acids and constituted of a labeled nucleotide strand having three fragments: a first fragment having a first closing sequence, a second fragment, all or part of which has a recognition sequence for the molecular recognition of the target nucleic acid and a third fragment having a second closing sequence, and at least two markers, one of the ends of the strand of the detection probe being free of any marker, in which, when the two closing sequences are hybridized together, the detection probe has a completely circular shape, the closing sequences thus maintaining the probe in a conformation that cannot be detected in the absence of the target nucleic acid.
    Type: Grant
    Filed: July 3, 2009
    Date of Patent: March 4, 2014
    Assignee: bioMerieux
    Inventors: Ali Laayoun, Eloy Bernal Mendez
  • Patent number: 8663924
    Abstract: A method for determining an efficiency of target enrichment from a DNA library, includes: adding a negative control sequence and a positive control sequence to the DNA library, or picking a negative control sequence and/or a positive control sequence from the library; determining a pre-capture amount of the negative control sequence and a pre-capture amount of the positive control sequence; performing enrichment of a target sequence from the DNA library using a bait sequence to produce a post-capture library; determining a post-capture amount of the negative control sequence and a post-capture amount of the positive control sequence in the post-capture library; and determining the efficiency of the target enrichment, based on the post-capture amount of the positive control sequence, the post-capture amount of the negative control sequence, the pre-capture amount of the positive control sequence, and the pre-capture amount of the negative control sequence.
    Type: Grant
    Filed: November 30, 2011
    Date of Patent: March 4, 2014
    Assignee: Agilent Technologies, Inc.
    Inventors: Scott Happe, Joseph Hoang Hai Ong
  • Patent number: 8663925
    Abstract: The invention relates to a method of preparing a reaction mixture for Polymerase Chain Reaction (PCR) assay and a solution set for PCR. The method comprises providing a sample solution comprising a biological sample to be amplified in said PCR assay and first colorant providing the solution a first color, providing a reagent solution comprising at least one other substance required for performing said assay and second colorant providing the solution a second color different from the first color, and mixing the sample solution and the first reagent solution for providing a mixed solution to be subjected to the PCR process, the mixed solution having, due to said first and second colorants, a third color different from the first and second colors. The invention significantly aids in pipetting PCR assays.
    Type: Grant
    Filed: October 4, 2010
    Date of Patent: March 4, 2014
    Assignee: Thermo Fisher Scientific Baltics UAB
    Inventors: Jaakko Kurkela, Katja Eklin, Sanna Uusivirta
  • Patent number: 8663926
    Abstract: One major problem in diagnosis methods presently available for anthrax is that these methods require several days to produce a result. The only existing treatment for anthrax requires administration soon after infection at a time when patients are exhibiting only mild flu-like symptoms. Thus, a patient may be days beyond the time when treatment would be effective by the time a diagnosis is made. The present invention reduces diagnosis time to as little as four hours providing same day identification of anthrax radically increasing the odds of delivering proper treatment and patient recovery. The rapid identification of anthrax lethal factor activity exhibited by the instant invention is also amenable to in vivo screening protocols for the discovery and development of anthrax vaccines and lethal factor inhibitors. The instant invention isolates and concentrates lethal factor and lethal toxin from nearly any biological sample.
    Type: Grant
    Filed: February 15, 2007
    Date of Patent: March 4, 2014
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services, Centers for Disease Control and Prevention
    Inventors: Anne E. Boyer, Conrad P. Quinn, John R. Barr
  • Patent number: 8663927
    Abstract: The presently-disclosed subject matter provides systems, methods, and kits for diagnosing and/or monitoring a bacteria-related condition of interest in a subject by providing a cell sensing system, each system containing a reporter molecule capable of detecting binding of a quorum sensing molecule and capable of generating a detectable signal.
    Type: Grant
    Filed: September 10, 2008
    Date of Patent: March 4, 2014
    Assignee: University of Kentucky Research Foundation
    Inventors: Sylvia Daunert, Sapna K. Deo, Patrizia Pasini, Anjali Kumari Struss, Harohalli Shashidhar, Deborah R. Auer Flomenhoft, Nilesh Raut
  • Patent number: 8663928
    Abstract: Method for the detection in homogeneous medium of a post-translational modification of a proteinaceous substrate catalyzed by a cell enzyme, characterized in that the post-translational modification reaction takes place in intact living cells, in that these cells comprise a heterologous expression vector coding for a fusion protein comprising the proteinaceous substrate and a first coupling domain and in that it comprises the following stages: (i) Incubation of the cells in the presence or in the absence of a compound to be tested capable of modulating the activity of said enzyme, (ii) Addition to the reaction medium of a first fluorescent compound member of a first pair of FRET partners covalently bonded to a coupling agent capable of binding specifically to the first coupling domain present on the proteinaceous substrate, (iii) Addition to the reaction medium of a second fluorescent compound member of this first pair of FRET partners, and covalently bonded to a binding domain specific to the site of the
    Type: Grant
    Filed: November 27, 2007
    Date of Patent: March 4, 2014
    Assignee: CIS BIO International
    Inventors: Eric Trinquet, Achim Brinker, Emmanuel Claret, Gérard Mathis
  • Patent number: 8663929
    Abstract: The present invention relates to an in-vitro immunoassay method for detecting the presence of liver cancer cells in a subject. In the method of the present invention, antigen retrieval treatment based on heat-induced epitope retrieval method and antigen retrieval treatment based on protease-induced epitope retrieval method can be combined in the detection of glypican 3 antigen expression in liver cancer tissues to thereby detect the difference in the expression level or expression pattern of the glypican 3 antigen by immunohistochemical staining method. This enables samples, which has been determined by the conventional HIER method as highly expressing glypican 3, to be graded according to the expression level of glypican 3.
    Type: Grant
    Filed: March 16, 2009
    Date of Patent: March 4, 2014
    Assignees: University of Miyazaki, Chugai Seiyaku Kabushiki Kaisha
    Inventors: Hiroaki Kataoka, Hirotake Takai, Atsuhiko Kato, Masami Suzuki, Masamichi Sugimoto
  • Patent number: 8663930
    Abstract: The invention relates to the field of medicinal research, cartilage physiology and diseases involving the degeneration of cartilage tissue. More specifically, the invention relates to methods and means for identifying compounds that inhibit catabolic processes in chondrocytes and that decrease the degradation of cartilage and/or ECM. The invention also relates to the compounds that are useful in the treatment of osteoarthritis. The invention also relates to targets, the modulation of which results in a decrease in the degradation of ECM and/or cartilage and decrease inflammation. In addition, the invention relates to compositions and methods for the use thereof in treating conditions that are characterized by the degradation of ECM and/or cartilage and inflammation.
    Type: Grant
    Filed: April 1, 2010
    Date of Patent: March 4, 2014
    Assignee: Galapagos NV
    Inventors: Reginald Christophe Xavier Brys, Luc Nelles, Nick Ernest Rene Vandeghinste, Blandine Mille-Baker, Michela Angela Tessari
  • Patent number: 8663931
    Abstract: This invention provides a diagnostic kit and a diagnostic marker used for diagnosing a renal disease. This invention also provides a method for detecting a renal disease comprising measuring at least one type of human megalin existing in urine selected from among full-length human megalin and human megalin fragments of (i) to (iii): (i) full-length human megalin; (ii) a human megalin endodomain fragment lacking a human megalin ectodomain; and (iii) a human megalin ectodomain fragment lacking a human megalin endodomain.
    Type: Grant
    Filed: April 27, 2010
    Date of Patent: March 4, 2014
    Assignees: Niigata University, Juntendo Educational Foundation, Denka Seiken Co., Ltd.
    Inventors: Akihiko Saito, Yasuhiko Tomino, Katsuhiko Asanuma, Shinya Ogasawara, Hiroyuki Kurosawa, Yoshiaki Hirayama
  • Patent number: 8663932
    Abstract: The present disclosure relates to methods for identifying proteins or peptide motifs of intracellular, extracellular, or extracellular matrix proteins specifically exposed in wound sites, as well as compositions for treating wounds, and methods for their use.
    Type: Grant
    Filed: December 21, 2011
    Date of Patent: March 4, 2014
    Assignee: Leidos, Inc.
    Inventors: John Dresios, Richard Griffey
  • Patent number: 8663933
    Abstract: Provided is a recombination protein which binds specifically to troponin I derived from human myocardium. The recombinant protein includes a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65.
    Type: Grant
    Filed: November 19, 2012
    Date of Patent: March 4, 2014
    Assignee: Panasonic Corporation
    Inventor: Atsushi Fukunaga
  • Patent number: 8663934
    Abstract: The instant invention relates to the use of 24-hydroxylated vitamin D compounds as therapeutics in mammalian bone fracture repair. In addition, the instant invention relates to novel 24-hydroxylated vitamin D compound receptors which can be employed in the development of compounds capable of facilitating fracture repair in animals. The instant invention also relates to nucleic acids encoding such receptors as well as vectors, host cells, transgenic animals comprising such nucleic acids and screening assays employing such receptors.
    Type: Grant
    Filed: May 30, 2013
    Date of Patent: March 4, 2014
    Assignee: Shriners Hospital for Children
    Inventor: Rene St-Arnaud
  • Patent number: 8663935
    Abstract: Novel compositions containing (i) ligand conjugated phycobiliprotein tandem dyes and (ii) unconjugated phycobiliproteins are provided wherein the phycobiliproteins are derived from the same bacterial or eukaryotic algae species. The phycobiliproteins must be the same or different, but must contain some non-crosslinked subunits which exchange. Also provided are methods for preparing these compositions, kits containing these compositions or components of the same, and methods of using these compositions for cellular and non-cellular analysis.
    Type: Grant
    Filed: December 10, 2009
    Date of Patent: March 4, 2014
    Assignee: Beckman Coulter, Inc.
    Inventors: Joseph Wolfers, Fabrice Malergue, Ravinder K. Gupta, Lori A. Charie, Francisco Estevez-Labori, Meryl Forman, Emmanuel Gautherot
  • Patent number: 8663936
    Abstract: The present invention is directed to a splice variant of a human sodium channel alpha subunit and methods and compositions for making and using the same.
    Type: Grant
    Filed: July 25, 2012
    Date of Patent: March 4, 2014
    Assignee: Vertex Pharmaceuticals Incorporated
    Inventors: Kenneth John McCormack, Christopher Dinesh Raj
  • Patent number: 8663937
    Abstract: The instant invention relates to the use of 24-hydroxylated vitamin D compounds as therapeutics in mammalian bone fracture repair. In addition, the instant invention relates to novel 24-hydroxylated vitamin D compound receptors which can be employed in the development of compounds capable of facilitating fracture repair in animals. The instant invention also relates to nucleic acids encoding such receptors as well as vectors, host cells, transgenic animals comprising such nucleic acids and screening assays employing such receptors.
    Type: Grant
    Filed: May 30, 2013
    Date of Patent: March 4, 2014
    Assignee: Shriners Hospital For Children
    Inventor: Rene St-Arnaud
  • Patent number: 8663938
    Abstract: Methods and compositions are provided for detection of biological substances in nasal specimen.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: March 4, 2014
    Inventor: Robert I. Henkin
  • Patent number: 8663939
    Abstract: The present invention provides a method of diagnosing a cyathostomin infection, said method comprising the step of identifying a level of anti-cyathostomin larval antigen antibodies in a sample, wherein a level of anti-cyathostomin larval antigen antibodies is indicative of a cyathostomin infection.
    Type: Grant
    Filed: March 31, 2010
    Date of Patent: March 4, 2014
    Assignee: Moredun Research Institute
    Inventors: Jacqui Matthews, Jane Hodgkinson, Christopher Proudman