Patents Issued in August 28, 2014
  • Publication number: 20140242548
    Abstract: A system for attaching a natural tooth to a dental implant. The system comprises a dental prosthesis and an elastomeric cushion. The dental prosthesis has a first end and a second end. The first end is fixed to a natural tooth and the second end is engaged with a dental implant. The elastomeric cushion is positioned in contact, either directly or indirectly, with at least a portion of the dental implant and at least a portion of the dental prosthesis such that the elastomeric cushion permits movement of the second end relative to the dental implant in response to movement of the natural tooth transferred through the dental prosthesis to the second end.
    Type: Application
    Filed: May 7, 2014
    Publication date: August 28, 2014
    Inventor: JAMES MICHAEL RANDALL
  • Publication number: 20140242549
    Abstract: An artificial teeth packaging container includes an artificial teeth accommodating portion for fixing a plurality of continuously arranged artificial teeth detachably, and holding an arrangement position relation with an adjacent artificial teeth and an arrangement position relation with an upper or lower artificial molar teeth, in which the artificial teeth accommodating portion covers the plurality of artificial teeth from an occlusal plane in a state exposed around a cervical portion.
    Type: Application
    Filed: May 8, 2014
    Publication date: August 28, 2014
    Applicant: KABUSHIKI KAISHA SHOFU
    Inventors: Hisashi OKADA, Noriyuki NEGORO, Kunihiro FUJII, Hirokazu SATO
  • Publication number: 20140242550
    Abstract: A dental prosthesis, includes a metal framework having at least one cap, which can be joined to an abutment of a dental implant. The metal framework includes a connecting element which can be, or is, joined to the cap in the oral cavity of a patient, in particular by way of welding, soldering or gluing, wherein the cap has a tip and, on the inside, a cavity into which the abutment can be inserted through an opening located opposite the tip. It is the object to refine the dental prosthesis and/or the metal framework so as to allow improved handling and/or facilitated creation of the, in particular intra-oral, preferably welded, joint with low design complexity. This object is attained by providing a pin arranged as an axial extension in the region of the tip of the cap and the connecting element is joined to the pin.
    Type: Application
    Filed: October 25, 2012
    Publication date: August 28, 2014
    Inventor: Marco Degidi
  • Publication number: 20140242551
    Abstract: An oral care system and method for the treatment of certain oral pathological conditions that require the cleaning and cleansing of the underlying scaffolding of the tooth. The oral care system and method generally includes applying chlorine dioxide liquid or gel to a diseased portion of the tooth and activating it so as to clean the aforementioned. A tool is utilized to activate the chlorine dioxide liquid thereby releasing its cleaning action. Another process utilizes repetitive steps of water and chlorine dioxide activated with a tool to cleanse the inner regions of an affected tooth.
    Type: Application
    Filed: February 28, 2014
    Publication date: August 28, 2014
    Inventor: Richard D. Downs
  • Publication number: 20140242552
    Abstract: The present invention relates to calcium carbonate particles, a powder mixture comprising said particles and a tooth cleaning method wherein the particles or powder mixture are used.
    Type: Application
    Filed: September 12, 2012
    Publication date: August 28, 2014
    Inventor: Christopher Louis Tsu
  • Publication number: 20140242553
    Abstract: A real-time simulation system of the aerodynamic loads generated by the wake of a rotor of a hover-capable aircraft on the aircraft itself is described, said system comprising: a cockpit seat for a pilot; a simulated control device of the aircraft able to receive a simulated command from the pilot to simulate a flight condition of the aircraft; simulation means able to generate a simulated representation of the flight condition; and a processing unit configured to receive as input a first signal associated with the command given via the control device and to generate and output a second control signal for the simulation means associated with the simulated aerodynamic loads. The processing unit cyclically generates a vortex ring with a radius, associates a number of control points with the vortex ring, computes the velocity induced on the control points, moves and updates the vortex ring, and generates the second signal on the basis of the velocities induced on the control points by the vortex rings.
    Type: Application
    Filed: July 18, 2012
    Publication date: August 28, 2014
    Inventors: Riccardo Bianco Mengotti, Francesco Scorcelletti
  • Publication number: 20140242554
    Abstract: System for enabling a visually impaired or blind person to use an input device having at least one key, comprising: at least one tactile sensor array, in particular a pressure sensor array, attachable to a fingertip of a visually impaired or blind person for acquiring a pressure distribution image of a key slightly touched by a finger of said person when attached to said finger, a pressure distribution image processing and character recognition unit that is connected to said tactile sensor array for processing said pressure distribution image and for recognizing the key label of the touched key and an audio output device that is connected to said unit for outputting and acoustic reproduction of said key label.
    Type: Application
    Filed: January 30, 2014
    Publication date: August 28, 2014
    Applicant: KING SAUD UNIVERSITY
    Inventor: Abdelhamid Mohamed MOSTAFA
  • Publication number: 20140242555
    Abstract: Disclosed is a Braille display device. The device supports an array of individual Braille cells with corresponding tactile pins. The pins can be selectively lifted by way of reeds to generate Braille characters that can be felt by the user. The Braille characters can correspond to visible characters, such as characters on a computer screen. The display is refreshable to allow for the sequential display of lines, paragraphs, or pages. In accordance with the disclosure, the Braille cells are constructed in a manner that minimizes manufacturing costs and that also permits the size of the display to be greatly reduced.
    Type: Application
    Filed: March 3, 2014
    Publication date: August 28, 2014
    Applicant: FREEDOM SCIENTIFIC, INC.
    Inventors: Patrick Murphy, Todd Conard, Waldemar H. Tunkis, Michael Goldenberg
  • Publication number: 20140242556
    Abstract: The present invention relates to a diet management system and method, wherein individual information data about users going through diet management are received and users are classified by body morphology, and a user-specific customised solution is provided in accordance with user body morphology classification-based diet targets, lifestyle analysis results and condition diagnostic data, and the user's current circumstances are received and a customised solution is provided that has been changed to match therewith. The present invention makes it possible to provide, in timely fashion, a solution wherein systemic diet management is effected that matches the characteristics of each individual person who is dieting, and wherein changes are effected so as to reflect the current condition of the person who is dieting.
    Type: Application
    Filed: November 15, 2012
    Publication date: August 28, 2014
    Applicant: AMOREPACIFIC CORPORATION
    Inventors: Lang Gook Yoo, Sung Hee Park, Jung Ki Min, Eun Kyung Seo, Hyun Woo Park, Sang Jun Lee, Sung Won Cho
  • Publication number: 20140242557
    Abstract: An audible dictionary device defined by a device housing having a multipart screen display, an lock button, a command button, a headphone jack, a keyboard, a speaker, and a detachable stylus. Internally, the audible dictionary device includes a power source, a processor, non-volatile memory, an internal database of reference information. In some embodiments, a network interface which enables the audible dictionary device to receive reference information from a network location is included in addition to or in the alternative of the internal database. The device executes a process by which words are looked up by a user, with the user then being provided with various reference information and be provided audibly in addition to on screen about the looked up word.
    Type: Application
    Filed: February 28, 2014
    Publication date: August 28, 2014
    Inventors: Aderonke Akinsanya, Sodjidassi Aziadapou
  • Publication number: 20140242558
    Abstract: A bartender training method and apparatus is provided, wherein the same can be deployed as a training course adapted to train users the art of mixology and efficient bartending skills. In an exemplary embodiment, the present invention contemplates one or more bar stations set up for making and serving mixed drinks, wherein each bar station comprises an interactive display screen and a slop bin to receive prepared drinks. The interactive display screen prompts each user with drink orders of varying complexity, whereby drink details may be provided for beginning learners and more difficult commands and routines are requested as experience is gained. Upon course completion, user performance data is used for grading purposes and for providing the user with a profile to show would-be employers. The present invention improves current methods of bartending training and emphasizes speed and efficiency of drinking making.
    Type: Application
    Filed: February 24, 2014
    Publication date: August 28, 2014
    Inventor: Mark Daragjati
  • Publication number: 20140242559
    Abstract: A method and system are disclosed for determining a task for a subject, which includes sensing electrical activity along a scalp of a subject; characterizing a mental state of the subject based on the electrical activity of the subject; comparing the mental state of the subject with a pre-defined task list to select a task for the subject based on the mental state of the subject; and assigning the task to the subject based on the comparison of the mental state of the subject and the pre-defined task list.
    Type: Application
    Filed: February 28, 2013
    Publication date: August 28, 2014
    Applicant: KONICA MINOLTA LABORATORY U.S.A., INC.
    Inventors: Ami Patel, Haisong Gu
  • Publication number: 20140242560
    Abstract: A machine learning classifier is trained to compute a quality measure of a facial expression with respect to a predetermined emotion, affective state, or situation. The expression may be of a person or an animated character. The quality measure may be provided to a person. The quality measure may also used to tune the appearance parameters of the animated character, including texture parameters. People may be trained to improve their expressiveness based on the feedback of the quality measure provided by the machine learning classifier, for example, to improve the quality of customer interactions, and to mitigate the symptoms of various affective and neurological disorders. The classifier may be built into a variety of mobile devices, including wearable devices such as Google Glass and smart watches.
    Type: Application
    Filed: February 17, 2014
    Publication date: August 28, 2014
    Applicant: Emotient
    Inventors: Javier MOVELLAN, Marian Steward BARTLETT, Ian FASEL, Gwen Ford LITTLEWORT, Joshua SUSSKIND, Ken Denman, Jacob WHITEHILL
  • Publication number: 20140242561
    Abstract: A medical non-invasive treatment of humans for treating spinal disorders, such as spinal curvature, osteochondrosis, scoliosis, and other related problems. The treatment includes formation of stable synaptic connections in the patient's mind by means of forming patient's conditional reflexes to the ideal way of performing an exercise, as shown by the expert. The treatment leads to formation of correct posture through the process comprising: patient being treated while standing, sitting or lying; a special film running on the screen, which shows an expert, who ideally performs exercises, and accentuates attention on certain parts of the spine by highlighting them and playing sounds of varying volume; the same parts of the patient's spine are being touched by the coach, a partner or the patient themselves simultaneously with the video.
    Type: Application
    Filed: February 26, 2014
    Publication date: August 28, 2014
    Inventor: Anna Dmitrievna Vladimirova
  • Publication number: 20140242562
    Abstract: A motion detection device that offers an audio/video reminder to wash the hands is disclosed. The device is desirable for use in public restrooms or other environments where frequent hand washing is required. The device provides a product that can effectively increase hygienic hand washing while educating patrons of proper hand washing techniques and optionally the health benefits and/or health code requirements of doing so.
    Type: Application
    Filed: February 27, 2014
    Publication date: August 28, 2014
    Applicant: SKANA Global, LLC
    Inventors: Kertia MCSTERLING, Nekisha Roache, Shonita Bond, Annette Gill, Anushka Clare
  • Publication number: 20140242563
    Abstract: A national standardized protocol developed by interfacing the FDA, medical societies, pharmaceutical companies, surgical companies, and patient focus groups for education patients about medications and surgical procedures which can be modified as new risks are discovered is desirable. Linking patient metadata to received information and then being able to identify patients about new risks and communicate to each patient is also desirable. Patients should receive all currently available information about a medication or surgical procedure and receive any newly discovered information.
    Type: Application
    Filed: February 23, 2014
    Publication date: August 28, 2014
    Applicant: Complete Consent, LLC
    Inventor: Sidney P. Smith
  • Publication number: 20140242564
    Abstract: A portable surgical training device is provided. The trainer includes a top cover spaced apart from a base to form a simulated body cavity for locating model organs that are substantially obscured from the field of view of the user. The top cover includes a video display, fixed insertion ports and interchangeable inserts containing simulated tissue layers. The training device has open sides for demonstrating and training lateral surgical techniques including a simulated or live tissue colon attached to a support leg for simulating transanal minimally invasive surgery. A training endoscope with an adjustable focal length for use with the trainer and, in particular, with optical trocars is disclosed. The surgical trainer can be angled and is well suited for training laparoscopic surgery techniques and demonstrating surgical instruments.
    Type: Application
    Filed: May 6, 2014
    Publication date: August 28, 2014
    Applicant: Applied Medical Resources Corporation
    Inventors: Boun Pravong, Kennii Pravongviengkham, Lee Cohen, Charles C. Hart, Vivek Sikri, Zoran Falkenstein, Eduardo Bolanos, Adam Hoke, Jacob J. Filek, Michael Palermo
  • Publication number: 20140242565
    Abstract: A system for managing course content for a course includes a database configured to store course content. Course content includes problem-solving learning environments and design (PSLED) blocks. An interface is configured to provide information from a PSLED block for a student. An input and output module are configured to receive and send input regarding at least one of the PSLED blocks stored in the database. An evaluation module is configured to evaluate the student based on assessment requirements in at least one of the PSLED blocks stored in the database and based on the assessment data. A mapping module is configured for analyzing and mapping attributes between PSLED blocks stored in the database, the attributes including at least one of equivalency of instructional units, equivalency of standards of learning and practice, equivalency of micro-certificates, and equivalency of micro-credentials.
    Type: Application
    Filed: February 25, 2014
    Publication date: August 28, 2014
    Inventor: Leigh Roy ABTS
  • Publication number: 20140242566
    Abstract: A central server outside of a school network receives assignments from educator computing devices, where each assignment includes content for distribution to student computing devices and a schedule indicating when the assignment is active for distribution. A local server within the school network receives the assignments and stores the assignments in a local server datastore. The local server receives from a gateway within the school network a request for active assignments, identifies the active assignments, and returns a list of the active assignments to the gateway. The gateway determines whether the list of the active assignments contains any assignments unretrieved by the given gateway. If so, the gateway sends to the local server a request for the unretrieved assignments. The gateway receives the requested assignments from the local server and stores them in a gateway datastore.
    Type: Application
    Filed: February 26, 2014
    Publication date: August 28, 2014
    Applicant: TV Band Service, LLC
    Inventors: Donald C. RICH, JR., Paul Allan SADOWSKI, Keith W. BOLICK, John M. CHAPIN
  • Publication number: 20140242567
    Abstract: A system for improving a student's knowledge or problem solving skills that identifies errors of a student's understanding of an underlying topic or application of a requisite skill needed to answer a test question correctly. The system includes a computer with a testing software program that presents a set of two or more questions regarding a topic to a student. Associated to the topic is a set of core concepts. Each question is associated with one or more core concepts and related with the other questions so that at least one core concept matches the core concept on the other questions. When the student incorrectly answers a question, the core concepts associated with the question is reviewed and the answers to the questions containing the matching core concepts are reviewed. If a question associated with the common core concept is previously answered correctly, the core concept associated exclusively to the question answered incorrectly is deemed the source of the error.
    Type: Application
    Filed: February 27, 2014
    Publication date: August 28, 2014
    Inventor: SEEOEN LEE
  • Publication number: 20140242568
    Abstract: The invention relates to an apparatus for temporarily displaying visual information, comprising a frame, a plate of material included in the frame that changes colour due to local pressure and when exposed to a magnetic field, assumes its original colour, a magnet movable along the plate, guide means for guiding the magnet along the sheet in a fast position, wherein the magnet is arranged for erasing a part of the plate by its magnetic field during the movement along the plate, whereby the guide means arc arranged for guiding the magnet along a path in a second position different from the first position, whereby the magnetic field generated by the magnet does not erase the first part of the plate and the device comprises operation means for operating between the first and second position of the magnet.
    Type: Application
    Filed: July 20, 2012
    Publication date: August 28, 2014
    Inventor: Franciscus Martinus Gerardus van Creij
  • Publication number: 20140242569
    Abstract: Methods for increasing breast muscle weight in poultry are disclosed herein. These methods can include delivery of compositions via in ovo administration. The methods disclosed herein also encompass the delivery of proteins. The methods disclosed herein are effective for increasing breast muscle weight in poultry, both in the embryo, and at slaughter age.
    Type: Application
    Filed: February 19, 2014
    Publication date: August 28, 2014
    Applicant: Zoetis LLC
    Inventor: Parag Chary
  • Publication number: 20140242570
    Abstract: In a method for picking up cell material from a culture dish a picking tool is displaced towards the cell material. Contact between the tool and the cell material is determined by carrying out capacitive measurements. After contact the tool is removed from the cell material. In an assembly for performing said method a support made of electrically conducting material supports the dish. A measurement device determines the start electrical capacity of the system composed of picking tool and support in a starting position of the tool, and determines the electrical capacity of the system when lowering the tool towards the dish. The measurement device provides signals representing the electrical capacity to a controller, which comprises a comparator for performing a comparison of the electrical capacity during lowering of the tool with the start electrical capacity. The controller controls the positioning of the tool at least based on this comparison.
    Type: Application
    Filed: September 28, 2012
    Publication date: August 28, 2014
    Applicant: BD KIESTRA B.V.
    Inventors: Jetze Botma, Martijn Kleefstra, Martijn Xander Berntsen, Tino Walter Van Der Zee
  • Publication number: 20140242571
    Abstract: An optical element includes a metal layer in which a first direction is a thickness direction; metallic particles provided to be spaced from the metal layer in the first direction; and a light transmitting layer that separates the metal layer from the metallic particles, in which the size T of the metallic particles in the first direction satisfies a relationship of 3 nm?T?14 nm, and the size D of the metallic particles in a second direction orthogonal to the first direction satisfies a relationship of 30 nm?D<50 nm.
    Type: Application
    Filed: February 25, 2014
    Publication date: August 28, 2014
    Applicant: Seiko Epson Corporation
    Inventor: Mamoru SUGIMOTO
  • Publication number: 20140242572
    Abstract: A chromatography method including a step of developing a complex of a specimen and a labeled substance modified by a first bondable substance which can be bonded to the specimen on an insoluble carrier, a step of capturing the complex of the specimen and the labeled substance at a reactive site on the insoluble carrier, a step of washing the reactive site on the insoluble carrier using a washing liquid, a step of detecting the specimen which is captured at the reactive site after a signal of the labeled substance is amplified by an amplification liquid, in which the reactive site on the insoluble carrier includes (i) a second bondable substance which can be bonded to the specimen or a substance having affinity to the first bondable substance which can be bonded to the specimen, and (ii) polyethylene glycol having the molecular weight from 1,500 to 10,000.
    Type: Application
    Filed: February 25, 2014
    Publication date: August 28, 2014
    Applicant: FUJIFILM Corporation
    Inventor: Atsuhiko WADA
  • Publication number: 20140242573
    Abstract: An optical element includes a metal layer having a thickness in a first direction; metallic particles spaced apart from the metal layer in the first direction; and a light transmitting layer separating the metal layer from the metallic particles. The metallic particles are disposed in a lattice shape in a second direction orthogonal to the first direction and in a third direction orthogonal to the first direction and the second direction. A distance between adjacent metal particles in the second and third directions is S, and 6 nm<S<40 nm.
    Type: Application
    Filed: February 26, 2014
    Publication date: August 28, 2014
    Applicant: Seiko Epson Corporation
    Inventor: Mamoru SUGIMOTO
  • Publication number: 20140242574
    Abstract: There has been a demand for a codon-optimized gene for the mutated catalytic domain of Oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and Escherichia coli. There has also been a demand for a substrate coelenterazine analogue showing a higher activity than that of native 19 kDa protein. The invention provides a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2. According to the invention, bis-coelenterazine is used as a substrate coelenterazine analogue suitable for the photoprotein encoded by the polynucleotide comprising the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2.
    Type: Application
    Filed: February 25, 2014
    Publication date: August 28, 2014
    Applicant: JNC CORPORATION
    Inventors: Satoshi INOUYE, Junichi SATO
  • Publication number: 20140242575
    Abstract: The invention provides inexpensive kits and methods for determination of an analyte in a sample with very high sensitivity. Analyte can be determined using an analyte binding member such as an antibody or an oligonucleotide, which can be directly or indirectly linked to an enzyme. The kit and method uses a compound that is altered in the presence of the enzyme, and that precipitates into crystalline particulates. The reaction composition with crystalline particulates is placed between two polarizers which are in an orthogonal arrangement, and the presence of particulates is observed, correlating to the analyte in the sample.
    Type: Application
    Filed: February 26, 2014
    Publication date: August 28, 2014
    Inventor: Gary Opperman
  • Publication number: 20140242576
    Abstract: The invention relates to methods to reduce the length of a homonucleotide repeat sequence a target DNA sequence. This is done by using an oligonucleotide primer which extends in the unaltered repeat of identical nucleotides of the target polynucleotide, the oligonucleotide primer comprises at the at the 5? side a sequence which hybridizes with the sequence preceding the targeted sequence repeat, but the oligonucleotide primer is not 100% complementary with the sequence repeat in the target sequence.
    Type: Application
    Filed: May 9, 2014
    Publication date: August 28, 2014
    Inventor: Harry CUPPENS
  • Publication number: 20140242577
    Abstract: Provided herein is a method for enriching a target nucleic acid molecule. In one embodiment, the method may involve hybridizing a C-probe to a strand of a target nucleic acid to produce a complex, enzymatically removing any 3? overhanging end from the target nucleic acid of the complex to produce a 3? hydroxyl group at the 3? end; extending the 3? end of the first sequence using the oligonucleotide sequence of the C-probe as a template; enzymatically removing any 5? overhanging end from the target nucleic acid, either before or after the extending step, to produce an 5? phosphate group at the end of the second sequence; and ligating the 5? phosphate group at the end of the second sequence to the 3? hydroxyl group at the end of the first sequence to produce a circular DNA molecule that contains the target sequence and the complement of the oligonucleotide sequence.
    Type: Application
    Filed: February 26, 2013
    Publication date: August 28, 2014
    Inventor: BRIAN JON PETER
  • Publication number: 20140242578
    Abstract: The present invention relates to a method for simultaneously detecting multiple miRNAs present in a sample and a kit for detecting same. According to the present invention, two or more miRNAs existing in trace amounts in a sample can be analyzed through only one measurement. The detection method of the present invention may be used for fast diagnosis of various diseases wherein miRNAs are involved, for example, cardiovascular diseases including myocardial infarction, with high accuracy.
    Type: Application
    Filed: April 18, 2013
    Publication date: August 28, 2014
    Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Eun Joo SONG, Eunmi Ban
  • Publication number: 20140242579
    Abstract: The present invention relates generally to methods of sequencing of polynucleotides and compounds, compositions and kits useful for sequencing of polynucleotides. The chemical compounds include nucleotide and nucleoside analogs which possess a blocking group covalently attached to the 3? hydroxyl of the sugar moiety. The blocking group may optionally be additionally covalently attached to a linker and/or detectable label. The nucleotide analogs may be ribonucleotide or deoxyribonucleotide analogs. Methods include incorporation of the reversible terminator molecules into growing polynucleotide strands by polymerase enzymes, such as in single base sequencing methodologies utilizing sequential reversible termination techniques.
    Type: Application
    Filed: July 26, 2013
    Publication date: August 28, 2014
    Inventors: Wei ZHOU, Glenn MCGALL, Moti JAIN, Rui MEI
  • Publication number: 20140242580
    Abstract: The invention provides a method for predicting the response of an EGFR-activating mutant subject suffering from a lung adenocarcinoma and receiving treatment with epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) and a method for predicting prognosis in an EGFR-activating mutant subject suffering from a lung adenocarcinoma and receiving treatment with EGFR-TKI. In the methods of the invention, clustered genomic alterations in specific chromosomes (in particular chromosomes 5p, 7p, 8q or 14q) are determined as a tool for predicting the response or prognosis.
    Type: Application
    Filed: July 5, 2012
    Publication date: August 28, 2014
    Applicant: NATIONAL TAIWAN UNIVERSITY
    Inventors: Sung-liang Yu, Pan-chyr Yang, Shinsheng Yuan, Gee-chen Chang, Hsuan-yu Chen, Ker-chau Li
  • Publication number: 20140242581
    Abstract: The present disclosure provides for compositions and methods for genetic analysis of embryos. Generally, the compositions and methods provide for the acquisition of an sample containing RNA from an embryo, genetic analysis involving various techniques such as sequencing-, hybridization- or amplification-based methods, and the detection of genetic alterations that may affect the health and quality of the embryo. In some cases, compositions and methods of this disclosure may provide information useful in the selection and monitoring of embryos for implantation into a female.
    Type: Application
    Filed: January 23, 2014
    Publication date: August 28, 2014
    Applicant: Reproductive Genetics and Technology Solutions, LLC
    Inventor: Mark T. Johnson
  • Publication number: 20140242582
    Abstract: The present invention provides assays systems and methods for detection of genetic variants in a sample, including copy number variation and single nucleotide polymorphisms. The invention preferably employs the technique of tandem ligation, i.e. the ligation of two or more fixed sequence oligonucleotides and one or more bridging oligonucleotides complementary to a region between the fixed sequence oligonucleotides combined with digital PCR detection.
    Type: Application
    Filed: February 18, 2014
    Publication date: August 28, 2014
    Applicant: Ariosa Diagnostics, Inc.
    Inventors: Arnold Oliphant, Jacob Zahn
  • Publication number: 20140242583
    Abstract: The present invention provides a method and single-tube assay for identification and quantitative analysis of differentially methylated MLH1 promoter sequences that are associated with certain types of cancer in an individual by obtaining a biological sample comprising DNA from the individual, detecting the presence of and measuring the level of methylated MLH1 promoter sequences, and comparing the presence of and level of methylation in the sample to a normalization reference of “normal” beta-actin gene promoters, wherein a difference in the level or pattern of MLH1 methylation of the sample compared to the Actin gene reference level identifies abnormally methylated MLH1 promoter sequences associated with cancer.
    Type: Application
    Filed: February 28, 2014
    Publication date: August 28, 2014
    Inventors: Jim Z. Lu, Zhongmin Guo
  • Publication number: 20140242584
    Abstract: The present invention is directed to a genomic DNA extraction reagent and method for improved extraction of DNA from biological tissue. The extraction reagent of the invention is mixed with disrupted biological tissue to form a DNA extraction solute which is incubated in a DNA extraction step. The extraction reagent includes an alkali component to maintain the DNA extraction solute at a pH of about 10 to 14 substantially throughout the extraction step. The extraction solute is centrifuged to clarify the supernatant. The supernatant containing the extracted DNA is diluted with a neutralizing buffer resulting in a high throughout method of generating high quantities of high quality DNA. Major PCR inhibitors are managed with the unique chemical combinations of the DNA extraction reagent designed and optimized for extraction of DNA from plant tissue and cells.
    Type: Application
    Filed: March 14, 2014
    Publication date: August 28, 2014
    Applicant: SYNGENTA PARTICIPATIONS AG
    Inventors: Yanshan Ji, Xiaoyin Fei, Wenjin Yu, Volker Mittendorf
  • Publication number: 20140242585
    Abstract: Genetic markers for identifying bovine carriers of complex vertebral malformation (CVM) disease gene are described. The genetic markers, including the microsatellite markers BM4129, INRAA003, BMS2790, ILSTS029, INRA123, BM220, HUJ246, BMS862, BMS937, BL1048, BMS2095 and BMS1266 and the bovine SLC35A3 gene, are located on bovine chromosome BTA3. The G/T polymorphism at position 559 of the bovine SLC35A3 gene is identified as being causative and diagnostic for CVM in cattle.
    Type: Application
    Filed: April 1, 2014
    Publication date: August 28, 2014
    Applicants: Aarhus Universitet, Viking Genetics FMBA
    Inventors: Christian Bendixen, Soren Svendsen, Helle Jensen, Frank Panitz, Anders Aasberg Pedersen, Lars-Erik Holm, Per Horn, Anette Hoj, Bo Thomsen, Mette Jeppesen, Birte Vivi Hunnicke Nielsen, Marc Ssergij Jonker
  • Publication number: 20140242586
    Abstract: Methods and compositions for nucleic acid amplification, detection, and genotyping techniques are disclosed. In one embodiment, a nucleic acid molecule having a target-specific primer sequence; an anti-tag sequence 5? of the target-specific primer sequence; a tag sequence 5? of the anti-tag sequence; and a blocker between the anti-tag sequence and the tag sequence is disclosed. Compositions containing such a nucleic acid molecule and methods of using such a nucleic acid molecule are also disclosed.
    Type: Application
    Filed: May 6, 2014
    Publication date: August 28, 2014
    Applicant: LUMINEX CORPORATION
    Inventors: Douglas F. WHITMAN, Hongwei Zhang
  • Publication number: 20140242587
    Abstract: The present invention is directed to devices, systems and methods that enable the detection of low copy numbers of bacterial polynucleotides in a sample without having to use multiple species specific primer sequences.
    Type: Application
    Filed: October 3, 2012
    Publication date: August 28, 2014
    Inventors: Kenneth H. Rand, Herbert J. Houck
  • Publication number: 20140242588
    Abstract: Technology provided herein relates in part to methods, processes and apparatuses for non-invasive assessment of genetic variations.
    Type: Application
    Filed: October 5, 2012
    Publication date: August 28, 2014
    Applicant: SEQUENOM, INC
    Inventors: Dirk Johannes Van Den Boom, Charles R. Cantor, Sung Kyun Kim, Zeljko Dzakula, Cosmin Deciu
  • Publication number: 20140242589
    Abstract: The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, utilize a of cyclic and/or non-cyclic solvent that is non-toxic and may eliminate or reduce the amount of formamide in the hybridization composition.
    Type: Application
    Filed: October 22, 2012
    Publication date: August 28, 2014
    Applicant: DAKO DENMARK A/S
    Inventor: Steen Hauge Matthiesen
  • Publication number: 20140242590
    Abstract: The invention provides methods and compositions for identifying acute myeloid leukemia (AML) patients that have an increased likelihood of a relapse following treatment with AC 220.
    Type: Application
    Filed: October 26, 2012
    Publication date: August 28, 2014
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Neil Pravin Shah, Catherine Choy Smith
  • Publication number: 20140242591
    Abstract: A method of providing protection against pneumococcal infection in a subject is disclosed. The method includes steps of administering to the subject a composition that includes combination of three recombinant pneumococcal neuraminidases: NanA, NanB, and NanC of S. pneumoniae strains CGSP14, wherein administration of the recombinant pneumococcal neuraminidases elicits an immune response to S. pneumoniae, and treats the subject. In one embodiment, the method further includes a step of adding adjuvants to enhance the immune response. The method also includes a step of using passive antibodies, wherein said passive antibodies are anti-neuraminidase antibodies generated from neuraminidases-immunized humanized animals: NanA, NanB, and NanC. Meanwhile, this invention also provides a method for the molecular diagnosis of pneumococcal infection.
    Type: Application
    Filed: February 25, 2013
    Publication date: August 28, 2014
    Applicant: CHANG GUNG MEDICAL FOUNDATION, LINKOU BRANCH
    Inventors: Cheng-Hsun Chiu, Rajendra Prasad Janapatla, Chyi-Liang Chen, Mei-Hua Hsu, Hsiu-Ling Chen, Chung-Tsui Huang, Hsin-Ju Chang, Wan-Ting Liao
  • Publication number: 20140242592
    Abstract: The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having a 3?-terminal nucleotide complementary to only one variant of the target sequence and having at least one nucleotide with a base covalently modified at the exocyclic amino group, wherein the allele-specific oligonucleotide is extended by a nucleotide-incorporating biocatalyst predominantly when hybridized to the variant of the target sequence for which it has said complementary 3?-terminal nucleotide.
    Type: Application
    Filed: October 11, 2013
    Publication date: August 28, 2014
    Applicant: Roche Molecular Systems, Inc.
    Inventors: Stephen G. Will, Alison Tsan, Nicolas Newton
  • Publication number: 20140242593
    Abstract: A sample chamber array is provided. The sample chamber array may comprise at least one reservoir in fluid communication with at least one sample chamber, and a movable portion defining the sample chamber. The reservoir is fillable with a liquid biological sample. The movable portion may be movable with respect to the remainder of the sample chamber from a first position to a second position. In the first position the movable portion is concave and the sample chamber is without biological sample. In the second position the movable portion is convex and the sample chamber comprises biological sample. The movement of the movable portion to the second position causes a pressure drop to transport the biological sample into the sample chamber from the at least one reservoir. Methods for processing a biological sample and methods of making a sample chamber array are also provided.
    Type: Application
    Filed: January 31, 2014
    Publication date: August 28, 2014
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventor: Donald R. Sandell
  • Publication number: 20140242594
    Abstract: A method for isolating genomic DNA (gDNA) from a biological material. In some embodiments, the method includes (a) contacting a sample that contains gDNA with a solution of hydroxide and a detergent under conditions and for a time sufficient to degrade a cell wall, a cell membrane, a nuclear membrane, a nucleoprotein, or combinations thereof and/or to denature the gDNA; (b) mixing into the solution resulting from step (a) a solution characterized by high salt and sufficient buffering capacity to reduce the pH of the solution to less than 10, thereby producing a neutralized solution; (c) centrifuging the sample at a speed and length of time sufficient to clarify the preparation; and (d) removing insoluble material from the neutralized and clarified preparation, whereby a solution of gDNA is produced.
    Type: Application
    Filed: February 4, 2014
    Publication date: August 28, 2014
    Applicant: SYNGENTA PARTICIPATIONS AG
    Inventors: Yanshan Ji, Wenling Wang, Er-Te Jamie Huang, Karen Elizabeth Moore, Kimberly Ann White
  • Publication number: 20140242595
    Abstract: The present invention provides methods comprising both genetic and chemical means for the production of hepatocytes from a variety of cell sources, particularly pluripotent stem cells.
    Type: Application
    Filed: February 21, 2014
    Publication date: August 28, 2014
    Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.
    Inventors: Junying YU, Xin ZHANG
  • Publication number: 20140242596
    Abstract: A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.
    Type: Application
    Filed: September 28, 2012
    Publication date: August 28, 2014
    Inventors: Ronald Danehy, Gary Lim, Jacob Freudenthal
  • Publication number: 20140242597
    Abstract: A diagnostic method for predicting quantitatively whether a human tumor will be sensitive or resistant to treatment with an ERBB3 inhibitor, e.g, an anti-ERBB3 antibody, is disclosed. The method is based on measurement of NRG1 expression at the RNA level, or at the protein level, in a tissue sample from the tumor.
    Type: Application
    Filed: October 5, 2012
    Publication date: August 28, 2014
    Applicant: AVEO PHARMACEUTICALS, INC.
    Inventors: Sylvie Vincent, Kristan Meetze, Bin Feng, Steven Tyler, Steve Bottega, Richard Nicoletti, Donna McIntosh, Jeno Gyuris