Abstract: The invention provides methods of detecting an EZH2 mutation and associated epigenetic markers in a cancer cell, methods cancer diagnosis and methods of screening for EZH2 inhibitors.

Abstract: The present invention relates to a method to determine the potency of a batch of glatiramer acetate comprising stimulating human monocytic cell line cells with an effective amount of interferon gamma (IFN?), exposing said cells to said batch of glatiramer acetate, and determining the expression of the monocyte anti-inflammatory cytokine sIL-1Ra or the viability of said cells induced by glatiramer acetate.

Abstract: Provided are multi-phase systems that may be used to prepare a three-dimension aggregate of cells referred to as a cellular spheroid. The multi-phase system includes a droplet of an aqueous polymer phase within an immersion aqueous polymer phase. The droplet of the droplet aqueous polymer phase contains a three-dimensional aggregate of cells (cellular spheroid). Types of cells that may be used in the multi-phases system include stem cells and cancer cells. The cellular spheroids with the multi-phase system may be used to monitor cell growth in three-dimensional systems, or screen drugs in a three-dimension aggregate of cells.

Abstract: Co-expression of T1R2 and T1R3 results in a taste receptor that responds to sweet taste stimuli, including naturally occurring and artificial sweeteners. Cells such as U2-OS, which express a functional sweet receptor, can be used in cell-based assays to detect cellular responses to tastants.

Abstract: The subject invention pertains to devices and methods of isolating target cells from a population of cells. The devices comprise of one or more microfluidic channels and scaffolding particles conjugated with one or more ligands that bind to the target cells. The scaffolding particles with one or more ligands are attached on to the surface of the one or more microfluidic channels. The methods of the current invention comprise passing the population of cells through the microfluidic channels of the devices of the current invention to facilitate interaction and capture of the target cells by the scaffolding particles-ligand conjugates, washing the device by a washing solution to remove the cells non-specifically bound to the scaffolding particle-ligand conjugates, releasing the captured target cells from the scaffolding particle-ligand conjugates, and collecting the released target cells.

Abstract: The present disclosure relates to the field of molecular biology and more specifically to methods for detecting anti-carbamylated protein (anti-CarP) antibodies in the serum of rheumatoid arthritis (RA) patients.

Abstract: A diagnostic test for celiac disease is disclosed. In particular, the invention relates to a method of diagnosing celiac disease by detecting activated, gut-bound CD8+ alpha-beta T lymphocytes and gamma-delta T lymphocytes in the peripheral blood of a subject who has consumed gluten for one to three days. This diagnostic test has a number of advantages over current tests for celiac test, including that the test is noninvasive, relatively inexpensive, and requires voluntary gluten ingestion over a short period of time.

Abstract: The present invention relates an in vitro method for detecting class II restricted CD4+ T cells in a sample. Herein a sample is contacted with an isolated complex of an MHC class II molecule and a peptide. This peptide comprises an MHC class II restricted T cell epitope of an antigenic protein and immediately adjacent thereof, or separated by a linker of at most 7 amino acids a sequence with a [CST]-xx-C or C-xx-[CST] motif. CD4+ T cells are detected by measuring the binding of the complex with cells in the sample, wherein the binding of the complex to a cell is indicative for the presence of CD4+ T cells in the sample. The present invention further relates to an isolated complex of an MHC Class II molecule and a peptide comprising an MHC class II restricted T cell epitope of an antigenic protein and immediately adjacent thereof, or separated by a linker of at most 7 amino acids a sequence with a [CST]-xx-C or C-xx-[CST] motif.

Abstract: This invention provides methods for determining or predicting response to HER2-directed therapy in an individual.

Abstract: Methods and kits for analysing a sample from a test subject. The methods involve determining the level of at least one compound selected from the group consisting of N-acetylglutamate, methionine, acetylcarnitine, indole-3-acetate, 2-oxoglutarate, anserine, aspartate and butyrate in the sample from the test subject; and comparing the level of the at least one compound determined to at least one control level, wherein the levels of the at least one compound are indicative of whether the subject has hepatic cancer.

Abstract: Disclosed viscosity-sensitive reporter compounds and methods allow tracing the changes in viscosity in a biological sample/environment (e.g., in medical, biochemical, biological, and/or pharmaceutical applications). Compounds and methods of the present disclosure can be used to identify the localization and the rheological state of hydrogels, their degradation products and other biostructural materials, including surgical implants, as well as the effectiveness of drug delivery by hydrogel carriers.

Abstract: A method of processing spectral data is disclosed and may include the steps of illuminating a medium to detect an inside particular component with light; obtaining a first spectral data for the medium at a first radial position and a second spectral data for the medium at a second radial position, wherein the first radial position and the second radial position are selected arbitrarily; and performing differential processing on the first spectral data and the second spectral data.

Abstract: Cardiovascular disease, e.g., congestive heart failure, is often first diagnosed after the onset of clinical symptoms, eliminating potential for early intervention. The invention provides a multi-marker immunoassay, including cardiac pathology and vascular inflammation biomarkers, yielding a more sensitive assay for early detection of CHF in plasma. A panel consisting of cardiac pathology (cTnI, BNP) and vascular inflammation (IL-6, TNF?, IL-17a) biomarkers provided a sensitivity of 94% for association with CHF.

Abstract: Methods for disease diagnosis, prognosis and therapy selection. Compositions for use in these methods and selected therapies for treatment are also disclosed.

Abstract: Biomarkers, methods, devices, reagents, systems, and kits used to assess an individual for the prediction of risk of developing a Cardiovascular (CV) Event over a 1 to 5 year period are provided.

Abstract: The present disclosure concern fragments of the chondroadherin (CHAD) polypeptide which are generated during the proteolytic cleavage of the third leucine repeat motif of the CHAD polypeptide. Such fragments are generated and thus associated with the onset and progression of intervertebral disc degeneration and can be used to assess the risk of a subject to develop intervertebral disc degeneration or to determine if the subject is afflicted by intervertebral disc degeneration. The fragments can also be used to screen for potential therapeutic agents for preventing, treating and/or alleviating the symptoms associated with intervertebral disc degeneration.

Abstract: The present invention relates to an in vitro method for diagnosing Crohn's disease or for determining predisposition in a person to develop Crohn's disease, by detecting an overexpression of the CD66c receptor in subjects suffering from said disease or at risk of developing the disease. The invention also relates to preventive or curative treatment for Crohn's disease.

Abstract: Methods of diagnosis or of quantitation of fibrosis are provided that comprise conducting an immunoassay to measure neo-epitope containing protein fragments naturally present in a biofluid sample, and associating an elevation of the measure in the patient above a normal level with the presence or extent of fibrosis. The immunoassay is conducted by a contacting protein fragments naturally present in the sample with an immunological binding partner reactive with a neo-epitope formed by cleavage of a protein by a proteinase and measuring the extent of binding of peptide fragments to the immunological binding partner to measure therein protein fragments comprising the neo-epitope. The protein is collagen type III, collagen type I, collagen type IV, collagen type V, elastin, biglycan, decorin, lumican, versican, perlecan, neurocan, brevican, fibromodulin, serglycin, syndecan, betaglycan, vimentin, or C-reactive protein.

Abstract: Compositions and methods for accurate and sensitive quantitation of T-tau and P-tau are disclosed.

Abstract: The present invention provides methods and uses for the diagnostic of a subject predisposed or suspected of developing a neurodegenerative disease or suffering from a neurodegenerative disease. The present invention also relates to methods and uses for identifying candidate compounds and to compounds for treating neurodegenerative disease. The present invention also relates to an animal model for neurodegenerative disease.

Abstract: Molecular-template polymer particles for a steroid hormone, said molecular-template polymer particles comprising a polymer that interacts with said steroid hormone. The polymerization unit of said polymer preferably contains at least two functional groups that interact with the aforementioned steroid hormone.

Abstract: A device that includes a first portion, the first portion including at least one fluid channel; a fluid actuator; and an introducer; a second portion, the second portion including at least one well, the well containing at least one material, wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel.

Abstract: The present invention relates to analytical testing devices including micro-environment sensors and methods for assaying coagulation in a fluid sample applied to the micro-environment sensors, and in particular, performing one or more types of coagulation assays using one or more micro-environment sensors in a single point of care combined test cartridge. For example, the present invention may be directed to test sensor including at least one transducer coated with a polymer layer. The polymer layer comprises a thrombin-cleavable peptide with a detectable moiety.

Abstract: The present invention relates to sample analysis cartridges comprising micro-environment sensors and methods for assaying coagulation in a fluid sample applied to the micro-environment sensors, and in particular, to performing coagulation assays using micro-environment sensors in a point of care sample analysis cartridge. For example, the present invention may be directed to a sample analysis cartridge including an inlet chamber configured to receive a biological sample, and a conduit fluidically connected to the inlet chamber and configured to receive the biological sample from the inlet chamber. The conduit may include a micro-environment prothrombin time (PT) sensor, and a micro-environment activated partial thromboplastin time (aPTT) sensor.

Abstract: The present invention relates to analytical testing devices comprising segmented fluidics and methods for assaying coagulation in a fluid sample received within the segmented fluidics. For example, the present invention may be directed to sample analysis cartridge including an inlet chamber, a first conduit comprising a first junction configured to split a biological sample into at least first and second segments, a second conduit comprising a first reagent, a first sensor region, and a first fluidic lock valve, and a third conduit comprising a first flow restrictor region, a second reagent, and a second sensor region. The sample analysis cartridge further includes a pump configured to independently mix the first segment in the second conduit and the second segment in the third conduit, and independently position the first segment over the first sensor region and position the second segment over the second sensor region.

Abstract: The present invention relates to analytical testing devices comprising fluidic junctions and methods for assaying coagulation in a fluid sample received within the fluidic junctions. For example, the present invention may be directed to a sample analysis cartridge including an inlet chamber, a first conduit comprising a first junction configured to split a biological sample into at least first and second segments, a second conduit comprising a first reagent, a first sensor region, and a first fluidic lock valve, and a third conduit comprising a second reagent, a second sensor region, and a second fluidic lock valve. The sample analysis cartridge further includes a pump configured to push the first segment over the first sensor region to the first fluidic lock valve, and push the second segment over the second sensor region to the second fluidic lock valve.

Abstract: The present invention relates to analytical testing devices comprising a single channel with micro-environment sensors and methods for assaying coagulation in a fluid sample applied to the micro-environment sensors, and in particular, to performing coagulation assays using a single channel with micro-environment sensors in a point of care test cartridge. For example, the present invention may be directed to a sample analysis cartridge including an inlet chamber configured to receive a biological sample and a conduit fluidically connected to the inlet chamber. The conduit includes a sensor chip including a first micro-environment sensor and a second microenvironment sensor, and a fluid lock valve.

Abstract: The present invention relates to ellagic acid formulations for performing coagulation assays that are highly stable for long term storage and reduce assay time. Particularly, aspects of the present invention are directed to a composition and method of preparing ellagic acid in a highly soluble format for use in a coagulation assay. For example, the ellagic acid may be solubilized in one or more of sodium hydroxide, methanol, a polyether compound, particularly polyethylene glycol, polyethylene oxide, or polyoxyethylene, and a cyclodextrin guest-host complex.

Abstract: Test strips for determining the activity of a coagulation factor in a blood sample are provided. The strip comprises a support, a sample inlet port for deposition of a blood sample, and a reaction area comprising a blood coagulation reagent. The sample inlet port is connected to the reaction area, and the coagulation reagent comprises blood plasma deficient in the coagulation factor for which activity is to be measured, an ionic citrate source an ionic calcium source, and either one or more coagulation contact phase activator reagents and phospholipids or a mixture of tissue factor and phospholipids. The disclosure further relates to in vitro methods for measuring an activity of a coagulation factor.

Abstract: Some embodiments of a blood coagulation testing system include an analyzer console device and a single-use cartridge component configured to releasably install into the console device. In some embodiments, the blood coagulation testing system can operate as an automated thromboelastometry system that is particularly useful, for example, at a point-of-care site.

Abstract: Some embodiments of a blood coagulation testing system include an analyzer console device and a single-use cartridge component configured to releasably install into the console device. In some embodiments, the blood coagulation testing system can operate as an automated thromboelastometry system that is particularly useful, for example, at a point-of-care site.

Abstract: Some embodiments of a blood coagulation testing system include an analyzer console device and a single-use cartridge component configured to releasably install into the console device. In some embodiments, the blood coagulation testing system can operate as an automated thromboelastometry system that is particularly useful, for example, at a point-of-care site.

Abstract: Some embodiments of a blood coagulation testing system include an analyzer console device and a single-use cartridge component configured to releasably install into the console device. In some embodiments, the blood coagulation testing system can operate as an automated thromboelastometry system that is particularly useful, for example, at a point-of-care site.

Abstract: Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

Abstract: Disclosed is a sample processing apparatus, comprising: a first mechanism unit having a first operation range of movement and that carries out a first process on a container with a sample, the first operation range comprising an overlap region and a non-overlap region; a second mechanism unit having a second operation range of movement and that carries out a second process on the container after completion of the first process, the second operation range comprising the overlap region but not the non-overlap region; an operation detector that senses operation of the first mechanism unit; and a controller that causes the first mechanism unit to stop the first process and retreat from the overlap region and that causes the second mechanism unit to continue the second process upon detection of abnormality in the first mechanism unit based on the detection result by the operation detector.

Abstract: An automatic apparatus is provided for screening disease/cancer in vitro. The apparatus comprises a filling device; a gripping device at a side of the filling device; a carrying platform correspondingly set under the filling device and the gripping device; a containing device on the carrying platform; at least two receiving slots on the carrying platform; a placing device on the carrying platform; a shaking device on the carrying platform; and a detecting device on the carrying platform. Thus, the apparatus is provided for molecular biological researches, biochemical examinations, radioimmunoassay and drug developments with reduced human labor, saved operating time, avoided experimental errors and enhanced experimental accuracy and efficiency.

Abstract: An acceleration measuring device to offset the limiting factors associated with the maximum acceleration an accelerometer can measure by attaching the device onto a mount system pre-calibrated to a specified angle relative to the horizontal axis of the object on which the system is mounted on.

Abstract: The invention relates to a measurement device for measuring the rotational speed of a vehicle wheel, the measurement device comprising a body (22) incorporating: a rotor (30) which can be rotated by the wheel and on which at least one permanent magnet (33) is mounted; a stator (31) comprising a winding (36) generating a measurement voltage when the wheel (2) and therefore the permanent magnet turn, the measurement voltage being indicative of the rotational speed of the wheel; an electronic board (32) comprising processing means for processing the measurement voltage; power supply means designed to generate, from the measurement voltage, a power supply voltage intended to power the electronic board.

Abstract: A robust and compact speed sensor assembly that includes a Hall effect sensor with protective circuitry in a compact housing that can be attached to the wheel of a motorcycle in the vicinity of the brake rotor to present the sensor Hall plate in a proper transverse-to-target orientation to detect passing targets, such as the brake rotor mounting bolts or magnets.

Abstract: A low-noise and high-sensitivity inertial sensor is provided. On the assumption that a movable portion VU1 and a movable portion VU2 are formed in the same SOI layer, the movable portion VU1 and the movable portion VU2 are mechanically connected to each other by a mechanical coupling portion MCU even while these movable portions are electrically isolated from each other. Thereby, according to a sensor element SE in the invention, it is possible to further suppress a shift between the capacitance of a MEMS capacitor 1 and the capacitance of a MEMS capacitor 2.

Abstract: An acceleration sensor that achieves a simultaneous operation method of a signal detection and a servo control is provided as an alternative to a time-division processing method. The acceleration sensor is a MEMS capacitive acceleration sensor. The acceleration sensor includes signal detection capacitor pairs 12, 15, and DC servo control capacitor pairs 13, 16, and AC servo control capacitor pairs 14, 17, which are different from the signal detection capacitor pairs 12, 15. A voltage that generates a force in a direction opposite to a detection signal of acceleration detected by the signal detection capacitor pairs 12, 15 is applied to the DC servo control capacitor pairs 13, 16 and the AC servo control capacitor pairs 14, 17.

Abstract: A configuration including a first substrate including a first movable electrode; a second substrate connected to the first substrate and including a first fixed electrode that faces the first movable electrode; and a third substrate connected to the second substrate. The first substrate, the second substrate, and the third substrate are laminated in this order, and the second substrate and the third substrate are not bonded to each other in at least a part between the first fixed electrode and the third substrate.

Abstract: A sensor device for an electronic apparatus is provided with: a sensing structure generating a first detection signal; and a dedicated integrated circuit, connected to the sensing structure, detecting, as a function of the first detection signal, a first event associated to the electronic apparatus and generating a first interrupt signal upon detection of the first event. The dedicated integrated circuit detects the first event as a function of a temporal evolution of the first detection signal, and in particular as a function of values assumed by the first detection signal within one or more successive time windows, and of a relation between these values.

Abstract: A microelectromechanical device includes: a supporting structure; two sensing masses, movable with respect to the supporting structure according to a first axis and a respective second axis; a driving device for maintaining the sensing masses in oscillation along the first axis in phase opposition; sensing units for supplying sensing signals indicative of displacements respectively of the sensing masses according to the respective second axis; processing components for combining the sensing signals so as to: in a first sensing mode, amplify effects on the sensing signals of concordant displacements and attenuate effects of discordant displacements of the sensing masses; and in a second sensing mode, amplify effects on the sensing signals of discordant displacements and attenuate effects of concordant displacements of the sensing masses.

Abstract: To calibrate an accelerometer, a rotating member is rotated over multiple periods, thereby causing the accelerometer attached to the rotating member to repeatedly turn over. A processor obtains acceleration measurements as the accelerometer turns and determines a set of local minima and maxima of the acceleration measurements. Based on these local minima and maxima, the processor determines a sensitivity of the accelerometer. The processor stores the sensitivity for use in adjusting subsequent accelerometer measurements, thus calibrating the accelerometer.

Abstract: In an aspect, in general, a system and method compensate for a misalignment characteristic of one or more acceleration sensors fixed to a sprung mass of a vehicle, each acceleration sensor having a location on the vehicle and a desired orientation relative to the vehicle.

Abstract: Provided is a test board including a main board which is configured to be connected to a plurality of devices-under-test (DUTs) and includes a plurality of test signal paths for transmitting a plurality of test signals input from an external tester to pins of at least one of the DUTs or transmitting a test result from the DUT to the tester, and a farm board which is connected to the main board and configured to mount therein a plurality of passive elements which are configured to be connected to at least one of the pins of the DUT through at least one of the test signal paths of the main board, when a test operation is performed, thereby improving a power integrity property or a signal integrity property in the test operation.

Abstract: Flexible film electrical-test substrates with at least one conductive contact post for integrated circuit (IC) bump(s) electrical testing, and related methods and testing apparatuses are disclosed. The backside structure of an electrical-test substrate comprises a flexible dielectric film structure. One or more fine-pitched conductive coupling posts are formed on conductive pads disposed on a front side of the flexible dielectric film structure through a fabrication process. A first pitch of the conductive coupling post(s) in the flexible dielectric film structure is provided to be the same or substantially the same as a second pitch of one or more bumps in an IC, such as die or interposer (e.g., forty (40) micrometers (?m) or less). This allows the conductive coupling post(s) to be placed into mechanical contact with at least one bump of the IC, point-by-point, during an electrical test to electrically testing of the IC.

Abstract: This detector (1) for an electric conductor comprises a frame (2) on which a mechanism unit (200) is fixed including a magnetic toroid, divided into two branches, the toroid having a coil (24) wound around at least one of the two branches, and a current sensor, divided into two branches (26A, 26B). The mechanism unit also includes a force transmitting element, said element being provided with at least one portion able to receive the conductor by bearing, and at least two clamps (30A, 30B) for anchoring the detector on the electric conductor, each clamp being rotatable between an open position and a locked position of the clamp. The detector also comprises a base and at least one electronic device (6). The force transmitting element is extended by a trigger, each anchoring clamp (30A, 30B) has a portion in contact with the trigger, and each clamp bears a protruding member (34A, 34B). A spring (36A, 36B) is stretched between the protruding member (34A, 34B) and a part (290B, 290B) fixed on the frame (2).

Abstract: A current sensor is provided. The current sensor includes a signal transmission unit through which a power signal is transmitted, a current measurement probe spaced apart from a side of the signal transmission unit and having a loop structure so as to partition an area which links together with a magnetic field induced according to the power signal transferred through the signal transmission unit, and a signal processing unit measuring an induced current induced at the current measurement probe by a magnetic field, which is generated according to the power signal transferred through the signal transmission unit, and calculating a current value of the power signal transferred into the signal transmission unit from a value of the measured induced current.