Abstract: An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns.

Abstract: The invention provides methods, databases and systems for making glycoprotein products having defined properties.

Abstract: A method of conducting an enzymatic reaction assay involving adenosine 5?-monophosphate (AMP) comprising the steps of reacting one or more compounds and producing AMP, deaminating the AMP to produce IMP, and oxidating the IMP by NAD+ to produce XMP and NADH.

Abstract: A novel class of hydroxylases is described having the amino acid sequence of SEQ ID NO: 2, 4, 6 and 8, and variants and fragments thereof having HIF hydroxylation activity. The polypeptides of the invention have in particular prolyl hydroxylase activity. An assay method monitors the interaction of the HIF hydroxylase with a substrate. Modulators of HIF hydroxylase are provided for use in the treatment of a condition associated with increased or decreased HIF levels or activity or for the treatment of a condition where it is desirable to modulate HIF levels or activity.

Abstract: A novel class of hydroxylases is described having the amino acid sequence of SEQ ID NO: 2, 4, 6 and 8, and variants and fragments thereof having HIF hydroxylation activity. The polypeptides of the invention have in particular prolyl hydroxylase activity. An assay method monitors the interaction of the IIIF hydroxylase with a substrate. Modulators of IIIF hydroxylase are provided for use in the treatment of a condition associated with increased or decreased HIF levels or activity or for the treatment of a condition where it is desirable to modulate HIF levels or activity.

Abstract: A novel class of hydroxylases is described having the amino acid sequence of SEQ ID NO: 2, 4, 6 and 8, and variants and fragments thereof having HIF hydroxylation activity. The polypeptides of the invention have in particular prolyl hydroxylase activity. An assay method monitors the interaction of the HIF hydroxylase with a substrate. Modulators of HIF hydroxylase are provided for use in the treatment of a condition associated with increased or decreased HIF levels or activity or for the treatment of a condition where it is desirable to modulate HIF levels or activity.

Abstract: A novel class of hydroxylases is described having the amino acid sequence of SEQ ID NO: 2, 4, 6 and 8, and variants and fragments thereof having HIF hydroxylation activity. The polypeptides of the invention have in particular prolyl hydroxylase activity. An assay method monitors the interaction of the IIIF hydroxylase with a substrate. Modulators of IIIF hydroxylase are provided for use in the treatment of a condition associated with increased or decreased HIF levels or activity or for the treatment of a condition where it is desirable to modulate HIF levels or activity.

Abstract: A novel class of hydroxylases is described having the amino acid sequence of SEQ ID NO: 2, 4, 6 and 8, and variants and fragments thereof having HIF hydroxylation activity. The polypeptides of the invention have in particular prolyl hydroxylase activity. An assay method monitors the interaction of the HIF hydroxylase with a substrate. Modulators of HIF hydroxylase are provided for use in the treatment of a condition associated with increased or decreased HIF levels or activity or for the treatment of a condition where it is desirable to modulate HIF levels or activity.

Abstract: Provided is a gold nanocage with pores, charged ligand molecules covalently bound to internal surfaces of the gold nanocage, and payload molecules electrostatically adsorbed onto said charged ligand molecules, wherein a pore diameter is between 1.2 and 20 times a gyration radius of the payload molecule. Also provided is a method for making a nanoparticle, including using polyvinylpyrrolidone as a capping agent in a galvanic replacement reaction to convert a silver nanocube into a gold nanocage having pores, replacing the polyvinylpyrrolidone on internal surfaces with charged ligand molecules, and electrostatically adsorbing payload molecules onto the charged ligand molecules, with a pore diameter less than twenty times a gyration radius of the payload molecule. Also provided is a method of delivering a pharmacological agent to a mammalian cell, including contacting the cell with a gold nanocage having pores, ligand molecules bound to internal surfaces, and pharmacological agent adsorbed onto ligand molecules.

Abstract: Methods for predicting the outcome of and monitoring prostate cancer patients using prostate specific antigen (PSA) proteolytic activity (PPA) in combination with other molecular biomarkers or other parameters are described. Methods of determining sets of parameters for use in predicting the outcome of and monitoring of prostate cancer patients are also described.

Abstract: Screening methods as well as systems and kits for identifying inhibitors of DNA-independent, histone H4-dependent activation of PARP-1 are provided. The methods comprise screening molecules for their capacity to inhibit the activation and/or biologic activity of PARP-1, as measured by poly(ADP)-ribose production from nicotinamide adenine dinucleotide. PARP-1 inhibitors identified through the screening methods may be used to treat cancer in which PARP-1 activation or biologic activity plays a role.

Abstract: A strategy to take advantage of time-resolved luminescence of Ln3+-chelated phosphotyrosine-containing peptides, which facilitate efficient energy transfer to small molecule fluorophores conjugated to the peptides to produce orthogonally-colored biosensors for two different kinases is provided. The method enables multiplexed detection with high signal to noise in a high-throughput-compatible format and a platform that could be applied to other lanthanide metal and fluorophore combinations to achieve even greater multiplexing without the need for phosphospecific antibodies.

Abstract: The methods and compositions for preparing cDNAs, and more particularly, compositions having propylene glycol for synthesizing a cDNA molecule or molecules from an mRNA template or population of mRNA templates under conditions sufficient to increase the detection sensitivity and cDNA yield and to simplify and improve the reliability of reverse transcription are provided. The reagent mixture comprises a ready to use reagent solution, wherein the solution comprises: (a) propylene glycol in a concentration between about 25% and about 50%; and (b) a viral reverse transcriptase selected from the group consisting of AMV RT, RSV RT, MMLV RT, HIV RT, EIAV RT, RAV2 RT, ASLV RT, RNaseH (?) RT, SuperScript II RT, and ThermoScript RT, in a buffer suitable for use in a reverse transcription reaction, wherein the buffer further comprises a co-factor metal ion and nucleoside triphosphates.

Abstract: The present invention relates to compositions and methods having propylene glycol and DNA polymerase for facilitating the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules, and for increasing the detection sensitivity and reliability through generation of secure cDNA molecules prior to gene-specific primer dependent amplification. The reagent mixture comprises a ready to use reagent solution, wherein the solution comprises: (a) propylene glycol in a concentration between about 20% and about 50%; (b) a viral reverse transcriptase; and (c) at least one DNA polymerases, in a buffer suitable for use in a reverse transcription reaction, wherein the buffer comprises a co-factor metal ion and nucleoside triphosphates.

Abstract: Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

Abstract: Novel methods of ChIP-seq are disclosed herein. These methods of ChIP-seq employ carrier DNA to prevent loss of DNA samples. The greater DNA yields achieved by this invention permit ChIP-seq of a small number of cells, permitting epigenetic analysis of primary cells of limited quantity.

Abstract: The invention relates to the use of inductively coupled plasma mass spectroscopy for cellular sample analysis. In some embodiments a method of performing mass spectroscopy analysis using an inductively coupled plasma mass spectroscopy system is provided. The method may include introducing a cellular sample comprising one or more cells or cellular particles into an inductively coupled plasma of the inductively coupled plasma mass spectroscopy system. The method may further include using the inductively coupled plasma mass spectroscopy system to assess the cellular sample by detecting and measuring one or more element tags in the cellular sample based on the element or isotopic compositions of the one or more element tags.

Abstract: This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.

Abstract: In some embodiments, the disclosure relates generally to methods, as well as compositions, systems, kits and apparatuses, for performing nucleotide incorporation, comprising: (a) providing a surface including one or more reaction sites containing a polymerase and a nucleic acid template that has, or is hybridized to, an extendible end; (b) performing a first nucleotide flow by contacting one or more of the reaction sites with a first solution including one or more types of terminator nucleotide; (c) incorporating at least one type of terminator nucleotide at the extendible end of the nucleic acid template contained within at least one of the reaction sites using the polymerase; and (d) detecting a non-optical signal indicating the nucleotide incorporation using a sensor that is attached or operatively linked to the at least one reaction site.

Abstract: The present invention provides methods of obtaining structural information about a biopolymer sample. The methods include labeling portions of a biopolymer, such as DNA or RNA, linearizing the biopolymer in some cases, and determining the distance between the labels. The user can then compare different samples' between-label distances to qualitatively compare different samples and to assay a given sample for additions or deletions of nucleotides in the regions flanked by the labels. The methods also permit sequencing of biopolymers.

Abstract: Computer implemented methods, and systems performing such methods for processing signal data from analytical operations and systems, and particularly in processing signal data from sequence-by-incorporation processes to identify nucleotide sequences of template nucleic acids and larger nucleic acid molecules, e.g.

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

Abstract: The present invention relates to a composition for detecting the undifferentiated human pluripotent stem cells comprising an agent useful for measuring the level of Desmoglein 2 (Dsg 2) mRNA or the protein thereof, a kit for detecting the undifferentiated human pluripotent stem cells comprising the said composition, a method for detecting the undifferentiated human pluripotent stem cells containing the step of measuring the level of Desmoglein 2 mRNA or the protein thereof, a method for evaluating the differentiation of human pluripotent stem cells and thereafter for separating the undifferentiated human pluripotent stem cells, a method for reducing the undifferentiated status of human pluripotent stem cells by inhibiting the expression or activation of Desmoglein 2, and a monoclonal antibody binding specifically to human Desmoglein 2.

Abstract: The present invention provides a method for determining P1/P2 blood type, including steps of providing a biological sample of a subject, detecting a genotype for single nucleotide polymorphism rs2143918 or rs5751348 in A4GALT gene of the biological sample and determining a phenotype of the subject based on the genotype. Further, the present invention also provides a kit for determining P1/P2 blood type, including a primer pair for detecting a genotype for single nucleotide polymorphisms rs2143918 or rs5751348 in A4GALT gene of a nucleic acid sample of a subject.

Abstract: The invention provides methods and compositions useful for detecting autoimmune disorders.

Abstract: Disclosed herein are biomarkers useful for identifying and/or classifying bacterial infections in a subject.

Abstract: The present invention provides a method of predicting an increased likelihood of a complication associated with administration of a bone morphogenetic protein (BMP) to a subject in need of a spinal fusion surgery by detecting single nucleotide polymorphisms (SNPs) in the subject. Also provided are methods of treatment with a BMP based on the presence or absence of SNPs and kits for use in detecting the SNPs.

Abstract: The present invention provides an aptamer specific to colorectal cancer cells and comprises a nucleotide sequence selected from the group consisting of Seq ID: NO. 1 to Seq ID: NO. 14. The aptamer of the present invention specific to colorectal cancer cell may be applied in colorectal cancer detection and provide a detection method having non-invasive, fast and convenient properties.

Abstract: The described invention provides methods for detecting, diagnosing and treating low-grade ovarian cancer and stage I ovarian cancer by comparing results from serum and ovarian tissue samples with normal controls. An increased level of expression of serine protease, wherein the serine protease is at least 2 selected from the group consisting of kallikrein 6 (KLK6), kallikrein 7 (KLK7), and PRSS8, expressed by subject samples compared to the level of expression of serine protease expressed by normal control samples is indicative of possible early stage ovarian cancer in the subject. Once early stage (I/II) ovarian cancer is diagnosed, the subject is treated with a treatment regimen effective to treat the early stage (I/II) ovarian cancer.

Abstract: The present disclosure provides a novel primer reagent for detecting a plurality of variants of the ALK fusion gene.

Abstract: Recurrent gene fusions of androgen regulated genes and ETS family member genes in prostate cancer are described. Compositions and methods having utility in prostate cancer diagnosis, research, and therapy are also provided.

Abstract: Molecular assays that involve measurement of expression levels of prognostic biomarkers, or co-expressed biomarkers, from a biological sample obtained from a prostate cancer patient, and analysis of the measured expression levels to provide information concerning the likely prognosis for said patient, and likelihood that said patient will have a recurrence of prostate cancer, or to classify the tumor by likelihood of clinical outcome or TMPRSS2 fusion status, are provided herein.

Abstract: The present disclosure relates to a method and system for identifying and treating corrosion within a conduit. The method involves using probes having a signal generator, and one or more corrosion mitigating chemical treatments such as biocides and/or corrosion inhibitors.

Abstract: A method for producing monosaccharides from algae includes (a) treating algae-containing water using an electrocoagulation device having an iron-based anode to induce flocculation of algae so as to obtain flocculated algae which have a trace amount of iron derived from the iron-based anode, (b) collecting the flocculated algae from the algae-containing water, and (c) subjecting the flocculated algae to an acid hydrolysis reaction in an acid solution to obtain monosaccharides. A liquid-to-solid ratio of a volume of the acid solution to a solid content of the flocculated algae is not less than 12 ml/g. The acid solution has an acid concentration not less than 3% by volume.

Abstract: The present invention provides processes for catalytic deconstruction of biomass using a solvent produced in a bioreforming reaction.

Abstract: Engineered animal skin, hide, and leather comprising a plurality of layers of collagen formed by cultured animal collagen-producing (e.g., skin) cells. Layers may be formed by elongate multicellular bodies comprising a plurality of cultured animal cells that are adhered and/or cohered to one another; wherein the elongate multicellular bodies are arranged to form a substantially planar layer for use in formation of engineered animal skin, hide, and leather. Further described herein are methods of forming engineered animal skin, hide, and leather utilizing said layers of animal collagen-producing cells.

Abstract: Provided is a method for manufacturing a rare-earth magnet capable of manufacturing a rare-earth magnet having excellent magnetic characteristics from magnetic powder that is prepared by liquid rapid-quenching and including both of nano-crystalline substance and amorphous substance as well. A method for manufacturing a rare-earth magnet includes: a first step of rapidly quenching of molten metal that is represented by a composition formula of (R1)x(Rh)yTzBsMt (R1 denotes one type or more of light rare-earth element containing Y, Rh denotes a heavy rare-earth element containing at least one type of Dy and Tb, T denotes transition metal containing at least one type of Fe, Ni and Co, B denotes boron, M denotes at least one type of Ga, Al and Cu, and 27?x?44, 0?y?10, z=100-x-y-s-t, 0.75?s?3.

Abstract: The invention discloses a steel plate resistant to zinc-induced crack and a manufacturing method therefor. A low-alloy steel subjected to low C-ultra low Si-high Mn-low Al—(Ti+Nb) microalloying treatment is taken as a basis; the Al content in the steel is appropriately reduced; the conditions are controlled so that Mn/C?15, [(% Mn)+0.75(% Mo)]×(% C)?0.16, Nb/Ti?1.8 and Ti/N is between 1.50 and 3.40, CEZ?0.44% and the B content is ?2 ppm, Ni/Cu?1.50; a Ca treatment is performed and the Ca/S ratio is controlled between 1.0 and 3.0, with (% Ca)×(% S)0.28?1.

Abstract: The invention relates to an alloy comprising (in mass %) Ni 33-35%, Cr 26-28%, Mo 6-7%, Cu 0.5-1.5%, Mn 1.0-4%, Si max. 0.1%, Al 0.01-0.3%, C max. 0.01%, N 0.1-0.25%, B 0.001-0.004%, SE>0 to 1%, and Fe remainder, including unavoidable impurities.

Abstract: The present invention relates to a high-strength spring steel having excellent wire-rod rolling properties, consisting essentially of, in terms of mass %: C: 0.40% to 0.65%; Si: 1.20% to 2.80%; Mn: 0.30% to 1.20%; P: 0.020% or less; S: 0.020% or less; Cu: 0.40% or less; Ni: 0.80% or less; Cr: 0.70% or less; Ti: 0.060% to 0.140%; Al: 0.10% or less; N: 0.010% or less; and O: 0.0015% or less, and optionally: B: 0.0005% to 0.0050%, with the remainder being Fe and inevitable impurities, in which the contents in terms of mass % of the specified chemical components satisfy the following Expressions (1) to (3): X1=0.14×[Si]?0.11×[Mn]?0.05×[Cu]?0.11×[Ni]?0.03×[Cr]+0.02?0.2 ??Expression (1) X2=(??500)/??3.0 ??Expression (2) ?=912?231×[C]+32×[Si]?20×[Mn]?40×[Cu]?18×[Ni]?15×[Cr] ?=10?(0.322?0.538×[C]+0.018×[Si]+1.294×[Mn]+0.693×[Cu]+0.609×[Ni]+0.847×[Cr]) X3=31×[C]+2.3×[Si]+2.3×[Mn]+1.25×[Cu]+2.68×[Ni]+3.57×[Cr]?6×[Ti]?24.0 ??Expression (3).

Abstract: A heat-resistant cold rolled ferritic stainless steel sheet containing, in mass %, 0.02% or less of C, 0.1% to 1.0% of Si, greater than 0.6% to 1.5% of Mn, 0.01% to 0.05% of P, 0.0001% to 0.0100% of S, 13.0% to 20.0% of Cr, 0.1% to 3.0% of Mo, 0.005% to 0.20% of Ti, 0.3% to 1.0% of Nb, 0.0002% to 0.0050% of B, 0.005% to 0.50% of Al, 0.02% or less of N, with the balance being Fe and inevitable impurities, in which {111}-oriented grains are present at an area ratio of 20% or greater in a region from a surface layer to t/4 (t is a sheet thickness), {111}-oriented grains are present at an area ratio of 40% or greater in a region from t/4 to t/2, and {011}-oriented grains are present at an area ratio of 15% or less in the entire region in a thickness direction.

Abstract: Provided is a Co—Ni-based alloy in which a crystal is easily controlled, a method of controlling a crystal of a Co—Ni-based alloy, a method of producing a Co—Ni-based alloy, and a Co—Ni-based alloy having controlled crystallinity. The Co—Ni-based alloy includes Co, Ni, Cr, and Mo, in which the Co—Ni-based alloy has a crystal texture in which a Goss orientation is a main orientation. The Co—Ni-based alloy preferably has a composition including, in terms of mass ratio: 28 to 42% of Co, 10 to 27% of Cr, 3 to 12% of Mo, 15 to 40% of Ni, 0.1 to 1% of Ti, 1.5% or less of Mn, 0.1 to 26% of Fe, 0.1% or less of C, and an inevitable impurity; and at least one kind selected from the group consisting of 3% or less of Nb, 5% or less of W, 0.5% or less of Al, 0.1% or less of Zr, and 0.01% or less of B.

Abstract: A method for fabrication of metal film with nanoapertures is provided. The method includes the steps of providing a nanopatterned template including a plurality of nanostructures, depositing of the metal film onto the nanopatterned template, and thermally induced dewetting of the metal film to define the nanoapertures in the metal film by diffusion and reflow of the metal film.

Abstract: In a method and apparatus for evaporation depositing uniform thin films, a film is deposited on a substrate of a vacuum environment while maintaining a constant deposition rate. A cover is installed on a wall of the evaporation vessel. When the evaporation material is heated to an evaporation state and the interior of the evaporation vessel reaches a first vapor saturation pressure, the vapor of the evaporation material flows towards a pressure stabilizing chamber. When the pressure stabilizing chamber reaches a second vapor saturation pressure which is smaller than the first vapor saturation pressure, the vacuum environment has a vacuum background pressure which is smaller than the second vapor saturation pressure, so that the evaporation material vapor flows from the pressure stabilizing chamber towards the vacuum environment at constant rate due to the pressure difference, so as to evaporate the substrate.

Abstract: A deposition system may have at least a substrate mounted within a sealed chamber. A radio frequency energy can be supplied to an inductive source affixed to the sealed chamber with the inductive source having an inductive coil surrounding a tube. Coupling the radio frequency energy into a gas pumped into the sealed chamber creates plasma to uniformly deposit a thin layer on a surface of the substrate.

Abstract: Methods and apparatus for cleaning an atomic layer deposition chamber are provided herein. In some embodiments, a chamber lid assembly includes: a housing enclosing a central channel that extends along a central axis and has an upper portion and a lower portion; a lid plate coupled to the housing and having a contoured bottom surface that extends downwardly and outwardly from a central opening coupled to the lower portion of the central channel to a peripheral portion of the lid plate; a first heating element to heat the central channel; a second heating element to heat the bottom surface of the lid plate; a remote plasma source fluidly coupled to the central channel; and an isolation collar coupled between the remote plasma source and the housing, wherein the isolation collar has an inner channel extending through the isolation collar to fluidly couple the remote plasma source and the central channel.

Abstract: Variable conductance gas distribution systems, reactors and systems including the variable conductance gas distribution systems, and methods of using the variable conductance gas distribution systems, reactors, and systems are disclosed. The variable conductance gas distribution systems allow rapid manipulation of gas-flow conductance through the gas distribution system.

Abstract: A vapor deposition method and apparatus including at least two vessels containing a same first source chemical. A controller is programmed to simultaneously pulse to the reaction space doses or pulses of a gas from the vessels, each of the doses having a substantially consistent concentration of the first source chemical. The apparatus may also include at least two vessels containing a same second source chemical. The controller can be programmed to simultaneously pulse to the reaction space doses or pulses of a gas from the vessels containing the second source chemical, each of the doses having a substantially consistent concentration of the second source chemical. The second source chemical can be pulsed to the reaction space after the reaction space is purged of an excess of the first source chemical.

Abstract: A heating module for use in a substrate processing chamber. The heating module having a housing with a heat source therein. The heating module can be part of a gas distribution assembly positioned above a susceptor assembly to heat the top surface of the susceptor and wafers directly. The heating module can have constant or variable power output. Processing chambers and methods of processing a wafer using the heating module are described.