Abstract: Described herein are methods and assays relating to the detection of cMET alterations (e.g. variations in copy number and expression level, and/or the presence of mutations, including point mutations). Existing methods are limited in their clinical usefulness by, e.g., limited sensitivity, inter-lab discordance, or inability to provide the necessary multiplex ability. The methods and assays provided herein permit multimodal, multiplex assaying for faster, more cost-effective testing and screening of patients, permitting improved healthcare.

Abstract: The present disclosure relates to methods for detecting a prostate cancer. Certain embodiments of the present disclosure provide a method of detecting a prostate cancer in a subject, the method comprising detecting a marker selected from an endosomal associated marker and/or a lysosomal associated marker from the subject.

Abstract: The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for assessing prostate cancer in a patient. In a specific embodiment, a method for predicting metastasis in a prostate cancer patient comprises the steps of (a) genotyping the ASPN D repeat domain length in both alleles of the patient using a polymerase chain reaction; (b) predicting metastasis in the patient if the ASPND repeat domain length is 13 in one allele and 14 in the other allele or have at least one allele with 14 D repeats as compared to other common allelic genotypes; and (c) predicting no metastasis in the patient if the ASPN D repeat domain length is 13 in both alleles as compared to other allelic genotypes.

Abstract: It has been found that BRG1 and BRM have a synthetic-lethal relationship, and that a treatment for inhibiting BRM is a promising approach for treating a BRG1-deficient cancer having no mutation in known therapeutic target genes. Moreover, it has also been revealed that, in this therapeutic strategy, a BRM inhibitor may be administered to a cancer patient after the selection based on BRG1 function suppression, thus enabling an efficient treatment based on the companion diagnosis.

Abstract: Compositions and fragment length analysis methods are provided for detecting CALR mutations and determining tumor load in patients with myeloproliferative neoplasms.

Abstract: The present invention provides systems useful for risk stratification of chronic lymphocytic leukemia (CLL) patients. The systems can include a microarray and a decision tree having steps for stratification of one or more CLL patients into prognostic groups. The invention further provides methods for risk stratification of CLL patients. The methods can include detecting the presence of alterations, such as copy number alterations, in sample genetic material from each of one or more CLL patients and then stratifying the one or more CLL patients into prognostic groups.

Abstract: The present invention provides compositions and methods for simultaneously detecting mutational status and gene copy number. In particular, the present invention provides simultaneous measurement of gene copy number and detection of the L858R and Exon 19 del mutations in a tissue sample.

Abstract: Herein is reported a method for determining methylation of a promoter nucleic acid operably linked to a nucleic acid encoding a polypeptide and thereby determining the long-term productivity of a cell. Also an aspect is a method for selecting a cell for producing a polypeptide by determining the methylation of the promoter nucleic acid operably linked to the structural gene encoding the polypeptide.

Abstract: Oligonucleic acid molecules comprising a SNP site at a position corresponding to position 7480 of the bovine signal transducer and activator of transcription (STAT5A) coding sequence (SEQ ID NO: 1). Also disclosed are an array or a kit comprising the same, a method for detecting the SNPs, a method for progeny testing of mammals, a method for increasing human and non-human mammal pregnancy rate in natural and artificial reproduction processes. Further provided are cattle breeding methods for improved milk production traits.

Abstract: Certain embodiments are directed to a sensitive isothermal amplification test based on the recombinase polymerase amplification (RPA) method that is capable of detecting <2 parasites in a biological sample.

Abstract: The present disclosure provides watermelon plants with a male sterile phenotype and their progeny. Such plants may comprise an introgressed genomic region associated with a male sterile phenotype. In certain aspects, compositions, including distinct polymorphic molecular markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a male sterile phenotype are provided.

Abstract: Various methods and compositions are provided for identifying and/or selecting soybean plants or soybean germplasm with resistance or improved resistance to soybean cyst nematode. In certain embodiments, the method comprises detecting at least one marker locus that is associated with resistance to soybean cyst nematode. In other embodiments, the method further comprises detecting at least one marker profile or haplotype associated with resistance to soybean cyst nematode. In further embodiments, the method comprises crossing a selected soybean plant with a second soybean plant. Further provided are markers, primers, probes and kits useful for identifying and/or selecting soybean plants or soybean germplasm with resistance or improved resistance to soybean cyst nematode.

Abstract: Methods for detecting the presence or absence of the swine H1N1 influenza A virus, seasonal H1 influenza A virus and/or seasonal H3 influenza A virus nucleic acids in biological samples are disclosed. Compositions that are target-specific nucleic acid sequences and kits comprising target-specific nucleic acid oligomers for amplifying in vitro the swine H1N1 influenza A virus, seasonal H1 influenza A virus and/or seasonal H3 influenza A virus nucleic acid and detecting amplified nucleic acid sequences are disclosed.

Abstract: Disclosed is a method in the fields of biochemistry and molecular biology. The method is related to improve cleavage kinetics of labeled oligonucleotide probes and, consequently, increases signal-to-noise ratio in detecting nucleic acids.

Abstract: A Si-killed steel wire rod for obtaining a spring excellent in fatigue properties and a spring excellent in fatigue properties obtained from such steel wire rod are provided. In the Si-killed steel wire rod of the present invention, oxide-based inclusions present in the wire rod contain SiO2: 30-90%, Al2O3: 2-35%, MgO: 35% or below (not inclusive of 0%), CaO: 50% or below (not inclusive of 0%), MnO: 20% or below (not inclusive of 0%) and BaO: 0.2-20% respectively, and total content of (CaO+MgO) is 3% or above.

Abstract: A method for producing a component by hot forming a pre-product made of steel is disclosed. The pre-product is heated to a forming temperature and is then reshaped, said component having a bainitic microstructure with a minimum tensile strength of 800 MPa after the forming process. In the process, the pre-product with the specified alloy composition is heated to a temperature below the Ac1 transformation temperature, said pre-product already consisting of a steel with a microstructure made of at least 50% bainite.

Abstract: Methods, systems, and apparatus, including an apparatus that is a rail anchor comprising a head, a tail, and a belly section. The belly section comprises a top surface, a bottom surface, and two side surfaces. Each side surface comprises a contact-bearing surface area. The head comprises a bend along a length of the head. The tail comprises a notch. Each contact-bearing surface area has a surface area of at least 3 square inches and is adapted to extend at least 1.5 inches downward from the top of a railroad track crosstie along a side of the railroad track crosstie.

Abstract: Systems and methods disclosed herein relate to the manufacture of metallic material with a thermal expansion coefficient in a predetermined range, comprising: deforming, a metallic material comprising a first phase and a first thermal expansion coefficient. In response to the deformation, at least some of the first phase is transformed into a second phase, wherein the second phase comprises martensite, and orienting the metallic material in at least one predetermined orientation, wherein the metallic material, subsequent to deformation, comprises a second thermal expansion coefficient, wherein the second thermal expansion coefficient is within a predetermined range, and wherein the thermal expansion is in at least one predetermined direction. In some embodiments, the metallic material comprises the second phase and is thermo-mechanically deformed to orient the grains in at least one direction.

Abstract: Provided is a porous metal body having superior corrosion resistance to conventional metal porous bodies composed of nickel-tin binary alloys and conventional metal porous bodies composed of nickel-chromium binary alloys. The porous metal body has a three-dimensional network skeleton and contains at least nickel, tin, and chromium. The concentration of chromium contained in the porous metal body is highest at the surface of the skeleton of the porous metal body and decreases toward the inner side of the skeleton. In one embodiment, the chromium concentration at the surface of the skeleton of the porous metal body is more preferably 3% by mass or more and 70% by mass or less.

Abstract: A method for post machining treatment of the interior surfaces of the access, suction, and discharge bores within each fluid chamber of the fluid end housing of a plunger pump that includes a three (3) step cold and hot working process. The treatment processes include a first shot peening of selected sections of said interior surfaces of each fluid chamber; a quench-polish-quench process whereby an anticorrosion nitride surface layer is added to the interior surfaces of each fluid chamber; and a second shot peening of the selected sections of the interior surfaces of each fluid chamber.

Abstract: A cold-rolled steel sheet includes a predetermined chemical composition, and includes a structure expressed by: an area fraction of ferrite: 95% or more; an area fraction of retained austenite and an area fraction of martensite: 1% to 3% in total; a product of the area fraction of retained austenite and a carbon concentration in retained austenite: 1 or more; a value of I(111)/{I(100)+I(110)} at a region where a depth from a surface is ¼ of a thickness of the cold-rolled steel sheet when intensity of a (hkl) plane is expressed by I(hkl): 2 or less.

Abstract: In a solvent extraction settler arrangement the outlet box comprises an inner tube arranged vertically inside a shaft, the inner tube being spaced from the side wall of the shaft to define an intermediate space between the inner tube and the shaft. The inner tube has an inner space and an opening at the lower part of the inner tube adjacent the bottom to form a flow path for the heavy solution phase to flow to the inner space. The shaft comprises a second outlet which is separate in relation to the discharge outlet and above the level of the discharge outlet. The second outlet opens through the side wall to the intermediate space at a location adjacent to the upper end of the shaft and at the level of said layer of entrained light solution phase for discharging said layer of entrained light solution phase from the intermediate space.

Abstract: A method to liberate and recover cesium, rubidium, or both from ore or other material is described. The method involves heating the ore or other material in the presence of at least one reactant. The heating is such that it liberates at least a portion of the cesium or rubidium or both from the ore. Cesium or rubidium or both resulting from the method are further described.

Abstract: A wrought material containing a Cu—Al—Mn-based alloy, in which an existence frequency of a coincidence grain boundary with a ? value of 3 or less is 35% or more but 75% or less, and which has a recrystallized microstructure substantially formed from a ? single phase; and the use thereof.

Abstract: Provided is a copper alloy for use in a member for water works, which has not only a reduced lead content and the lowest possible Ni content, but also a reduced Bi content, and which still exhibits suitable properties. The copper alloy includes: less than 0.5% by mass of Ni; 0.2% by mass or more and 0.9% by mass or less of Bi; 12.0% by mass or more and 20.0% by mass or less of Zn; 1.5% by mass or more and 4.5% by mass or less of Sn; and 0.005% by mass or more and 0.1% by mass or less of P; wherein the total content of Zn and Sn is 21.5% by mass or less, and the balance is a trace element(s) and Cu.

Abstract: Disclosed are a lead-free, high-sulphur and easy-cutting copper-manganese alloy and preparation method thereof. The alloy comprises the following components in percentage by weight: 52.0-95.0 wt. % of copper, 0.01-0.20 wt. % of phosphorus, 0.01-20 wt. % of tin, 0.55-7.0 wt. % of manganese, 0.191-1.0 wt. % of sulphur, one or more metals other than zinc that have an affinity to sulphur less than the affinity of manganese to sulphur, with the sum of the contents thereof no more than 2.0 wt. %, and the balance being zinc and inevitable impurities, wherein the metals other than zinc that have an affinity to sulphur less than the affinity of manganese to sulphur are nickel, iron, tungsten, cobalt, molybdenum, antimony, bismuth and niobium.

Abstract: Disclosed herein is an aluminum alloy composition and a method of heat treating the aluminum alloy, to improve process control and strength of the aluminum alloy for a rear safety plate mounted on a truck, etc., complying with safety regulations wherein the aluminum alloy composition includes Silicon (Si) about 0.8 to 1.3% by weight, Iron (Fe) up to about 0.5% by weight, Copper (Cu) about 0.15 to 0.4% by weight, Manganese (Mn) up to about 0.15% by weight, Magnesium (Mg) about 0.8 to 1.2% by weight, Chromium (Cr) up to about 0.25% by weight, Zinc (Zn) up to about 0.2% by weight, Titanium (Ti) up to about 0.1% by weight and the remaining percent by weight of Aluminum (Al) of the entire composition.

Abstract: A cermet contains hard phase particles containing Ti and a binding phase containing at least one of Ni and Co. 70% or more of the hard phase particles have a cored structure containing a core and a peripheral portion around the core. The core is composed mainly of at least one of Ti carbide, Ti nitride, and Ti carbonitride. The peripheral portion is composed mainly of a Ti composite compound containing Ti and at least one selected from W, Mo, Ta, Nb, and Cr. The core has an average particle size ?, the peripheral portion has an average particle size ?, and ? and ? satisfy 1.1??/??1.7. The hard phase particles in the cermet have an average particle size of more than 1.0 ?m.

Abstract: A cermet contains hard phase particles containing Ti and a binding phase containing at least one of Ni and Co, and 70% or more (by number) of the hard phase particles have a cored structure containing a core and a peripheral portion around the core. The core is composed mainly of at least one of Ti carbide, Ti nitride, and Ti carbonitride, and the peripheral portion is composed mainly of a Ti composite compound containing Ti and at least one selected from W, Mo, Ta, Nb, and Cr, The core has an average particle size ?, the peripheral portion has an average particle size ?, and ? and ? satisfy 1.1??/??1.7.

Abstract: An austenitic steel matrix-nanoparticle composite and a producing method thereof are provided. The composite includes: an austenitic steel matrix that includes an alloying element; and a nanoparticle that grows in situ in the matrix and that is formed in the matrix. The nanoparticle grows from the alloying element included in the austenitic steel matrix. The method includes: preparing an austenitic steel matrix including an alloying element; and heating the austenitic steel matrix. In the method, the nanoparticle grows in situ in the matrix from the alloying element which is solid-dissolved in the austenitic steel matrix by the heating.

Abstract: A system and method for manufacture of prosthesis shells using a hot liquid bath to cure the shells is described. The shell is formed on a mold or mandrel, and is then immersed in a hot liquid bath to cure the shell material. Multiple layers of material, such a silicone, may be formed by immersing the layer into a hot liquid bath, cooling the cured layer, applying another layer of uncured material to the cured layer, and then curing the uncured layer in a hot liquid bath. A textured surface may be imparted to the shell by incorporating a texture component into the last layer formed, such that immersing the uncured layer incorporating the texture component in the hot liquid bath cures the last layer formed and dissolves the texture component.

Abstract: A method of activating the surface of an aluminum-based substrate. This method includes cleaning the substrate surface, and operating a plasma spraying device such that a air plasma jet produced by the device is accelerated toward the surface so that it removes or decomposes any remaining oxides and other surface contaminants. In one form, the surface being treated forms part of a cylinder bore of an internal combustion engine block. In a particular form where a protective coating is subsequently being applied to the substrate, a sequential series of plasma spraying devices may be used such that a first—preferably simpler—device is used to perform activation or pretreatment operations, while a second—and more comprehensive—device may be used to deposit the protective layer on the pretreated surface.

Abstract: A disclosure is directed to a method for rapidly nitriding steel, the method including: placing the steel in a furnace having an atmosphere comprising partially dissociated ammonia gas; heating the steel to a highest temperature in a range of 400 to 600° C. while holding a nitriding potential below 15 atm?1/2 during heat-up from 400° C. up to the highest temperature; and holding the steel at the highest temperature while continuing to maintain the nitriding potential below 15 atm?1/2, where a total time taken for the heating and holding the steel in the range of 400 to 600° C. during the nitriding is 15 hours or less, and where a composition of the steel comprises at least one of the group consisting of Al, Cr, Mo, V, and Ti.

Abstract: According to one embodiment, a method of manufacturing a magnetoresistive memory device includes forming a first magnetic layer on a substrate, forming a cap layer on the first magnetic layer, heating a base including the cap layer after the cap layer is formed, forming a nonmagnetic layer on the cap layer while the base is heated, cooling the base including the nonmagnetic layer after the nonmagnetic layer is formed, and forming a second magnetic layer on the nonmagnetic layer after the base is cooled.

Abstract: An article includes a substrate, a Ti-bottom layer deposited on the substrate and a TiN/TiC coating deposited on the Ti-bottom layer. The TiN/TiC coating includes a plurality of TiN-nano layers and a plurality of TiC-nano layers. Each TiN-nano layer and each TiC-nano layers are alternately deposited on the Ti-bottom layer. The TiN/TiC coating has good toughness and high hardness. A method for manufacturing the TiN/TiC coating is also provided.

Abstract: Disclosed is a rotation/revolution type vapor phase growth apparatus that allows for automatic meshing between an external gear and an internal gear. In the apparatus, on tooth side surfaces of at least one kind of a plurality of external gear members provided rotatably in a circumferential direction of an outer periphery of a disk-shaped susceptor and a ring-shaped fixed internal gear member having an internal gear to mesh with the external gear members, there is provided a guide slope that abuts against a tooth side surface of the other kind of the gear member(s) to guide both kinds of the gear members into a meshed state when both kinds of the gear members move from a non-meshed state to the meshed state.

Abstract: Systems and methods of controlling a deposition rate during thin-film fabrication are provided. A system as provided may include a chamber, a material source contained within the chamber, an electrical component to activate the material source, a substrate holder to support the multilayer stack and at least one witness sample. The system may further include a measurement device and a computational unit. The material source provides a layer of material to the multilayer stack and to the witness sample at a deposition rate controlled at least partially by the electrical component and based on a correction value obtained in real-time by the computational unit. In some embodiments, the correction value is based on a measured value provided by the measurement device and a computed value provided by the computational unit according to a model.

Abstract: A process for applying a silicon oxide barrier coating to a PET container, wherein the PET container comprises a wall having an inner surface and an outer surface, the process comprising the steps of: (a) heating a PET container such that at least the outer surface is at a temperature of from about 200° F. to about 383° F.; (b) forming a coated PET container by applying at least one silicon oxide barrier layer on at least the inner surface of the PET container while the temperature of at least the outer surface of the PET container is at a temperature of from about 200° F. to about 383° F.; and (c) cooling the coated PET container after step b.

Abstract: A nanoparticles aerosol generator is disclosed. The nanoparticles aerosol generator includes an evaporation chamber having a wall, a container containing a source material, and a heating device configured to heat the source material. The nanoparticles aerosol generator also includes a carrier gas source configured to blow a carrier gas toward the source material to generate a nanoparticles aerosol with nanoparticles of the source material suspended therein. The nanoparticles aerosol generator further includes a dilution gas source configured to supply a dilution gas into the chamber to flow substantially along the wall within the chamber and to dilute the nanoparticles aerosol.

Abstract: Vapor deposition methods of cobalt-containing films by using cobalt carbonyl nitrosyl are disclosed.

Abstract: A deposition apparatus comprises a source unit configured to generate a plasma by arc discharge, a deposition unit in which a deposition target material is arranged so as to be irradiated with the plasma generated in the source unit, and an induction unit configured to induce the plasma for the source unit to the deposition unit. The induction unit comprises a partition unit airtightly connected to each of the source unit and the deposition unit and configured to pass the plasma inside, and a plurality of magnet units configured to form a magnetic field to induce the plasma in the partition unit. The plurality of magnet units are connected to adjust a connection angle, and the partition unit includes a tubular member bendable according to the connection angle of the plurality of magnet units.

Abstract: There is provided a gold plate coated stainless material characterized by comprising: a stainless steel sheet formed with a passivation film having a surface of which a Cr/O value is within a range of 0.05 to 0.2 and a Cr/Fe value is within a range of 0.5 to 0.8 when measured by Auger electron spectroscopy analysis; and a gold plated layer formed on the passivation film of the stainless steel sheet. According to the present invention, there can be provided a gold plate coated stainless material which can be improved in the coverage and interfacial adhesion property of the gold plated layer formed on the stainless steel sheet even when reducing the thickness of the gold plated layer, thereby to be excellent in corrosion resistance and conductivity and advantageous in cost.

Abstract: A method of operating an electroless plating apparatus is disclosed. The operating method includes: storing in the electroless plating apparatus an order of priority of the plurality of processes which has been predetermined based on a stability of a processed substrate with respect to pure water; supplying pure water into the holder storage bath when any of the plurality of processing baths malfunctions; determining whether or not a relieving process can be performed, the relieving process being a process of performing a higher-priority process on a substrate; if the relieving process can be performed, performing the relieving process and then immersing the substrate holder holding the substrate in the pure water in the holder storage bath; and if the relieving process cannot be performed, immersing the substrate holder, holding the substrate, in the pure water held in the holder storage bath without performing the relieving process.

Abstract: A nozzle assembly for a cold spray deposition system includes a nozzle body with an axial bore. The axial bore defines a converging segment, a diverging segment downstream of the converging segment, and a throat fluidly connected between the converging and diverging segments of the axial bore. A particulate conduit is fixed within the axial bore and extends along the axial bore diverging segment for issuing solid particulate into the diverging segment of the axial bore.

Abstract: The present invention relates to a protective layer for TiAl materials for affording protection against oxidation, said protective layer having a layer sequence which, proceeding from the inner side facing toward the TiAl material (1), has an inner aluminum oxide layer (5), a first gradient layer (6) comprising aluminum and a base metal with a base metal content increasing outward toward the surface side, a base metal layer (7), a second gradient layer (8) comprising aluminum and a base metal with an aluminum content increasing outward toward the surface side, and an outer aluminum oxide layer (9), and also to a method for the production thereof

Abstract: A method of applying a coating system to a substrate includes applying a first layer of a high hardness and high modulus of elasticity with an added metal to the substrate, applying a second layer of the high hardness and high modulus of elasticity in combination with the added metal to the first layer. A percent by volume of the added metal in the second layer is lower than the percent by volume of the added metal in the first layer. The method also includes applying two or more intermediate layers formed from an applied mixture of the high hardness, high modulus of elasticity material and a metal material between the first layer and the second layer.

Abstract: A method for manufacturing a porcelain enamel coating of a metal substrate comprises the steps of: preparing the surface of the metal substrate; applying a primer on the metal substrate; firing inside an oven at a temperature of between 750 and 900° C.; applying an enamel suspension comprising water and a glass frit; firing inside an oven at a temperature of between 700 and 900° C.; and repeating the last two steps according to the desired thickness of porcelain enamel. The method is characterized in that the glass frit comprises a quantity of lattice modifiers belonging to the transition metals, equal to less than 1% of the total volume of the glass frit; a quantity of opacifying oxides equal to less than 1% of the total volume of the glass frit is added to the glass frit. Moreover, the enamel suspension comprises a quantity of suspending agents with dimensions greater than the wavelength range of visible radiation, equal to less than 1% of the total volume of the glass frit.

Abstract: A rust inhibition system in which a rust inhibitor compound is applied to a wand, which is then inserted into a cavity. The wand serves as a reservoir to provide the inhibitor compound in areas susceptible to corrosion.

Abstract: A differential pressure water electrolysis system includes high-pressure water electrolysis cells and a high pressure hydrogen manifold. The high-pressure water electrolysis cells are stacked in a stacking direction. Each of the high-pressure water electrolysis cells includes an electrolyte membrane, an anode current collector, a cathode current collector, a tabular anode separator, a tabular cathode separator, a sealing member, and a resin frame member. The resin frame member is disposed between the tabular anode separator and the tabular cathode separator so as to surround the sealing member and the anode current collector. The resin frame member includes a water supply port to introduce water for electrolysis and a water discharge port to discharge a surplus of the water after electrolysis. The high pressure hydrogen manifold is provided so as to distribute hydrogen in the stacking direction and so as to be encircled by the sealing member.

Abstract: A hybrid electrochemical cell using reversible operation of a solid oxide cell includes: i) solid oxide cell generating power; ii) first storage container storing hydrogen and carbon monoxide discharged from the solid oxide cell supplying the hydrogen and carbon monoxide to the solid oxide cell; iii) second storage container storing steam and carbon dioxide discharged from the solid oxide cell supplying the steam and carbon dioxide to the solid oxide cell; iv) first connection pipe connecting the first storage container, the second storage container, and the solid oxide cell; v) second connection pipe connecting the first storage container, the second storage container, and the solid oxide cell; vi) discharging terminal connected to the solid oxide cell; vii) charging terminal connected to the solid oxide cell spaced apart from the discharging terminal, having the solid oxide cell disposed in between; and viii) mode converter connected to the solid oxide cell.