Abstract: A method for producing a transgenic plant which has increased content of 20-hydroxyecdysone compared to a wild type plant includes transforming a plant cell with a recombinant vector containing a gene encoding CYP85 (cytochrome P450, 85 family) protein derived from spinach (Spinacia oleracea). A method for producing a transgenic plant with enhanced insect resistance includes transformation of a plant cell with a recombinant vector containing a gene encoding CYP85 derived from Spinacia oleracea.
Type:
Application
Filed:
November 27, 2014
Publication date:
October 20, 2016
Inventors:
Key Zung RIU, Kyung Hwan BOO, Jong Cheol AHN
Abstract: The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.
Type:
Application
Filed:
June 28, 2016
Publication date:
October 20, 2016
Inventors:
Ignacio M. Larrinua, Donald Merlo, Avutu Sambi Reddy, Arvind Kumar ThirumalaiswamySekhar, Aaron T. Woosley
Abstract: The present invention relates to an expression vector having an improved ability to express a gene, cells transformed by the expression vector, and a method for mass-producing a target protein by using the cells. The expression vector according to the present invention shows a more excellent ability to express a gene than a typical animal cell expression vector, and thus, protein expression of a heterogeneous gene can be significantly increased.
Type:
Application
Filed:
December 29, 2014
Publication date:
October 20, 2016
Applicant:
MOGAM BIOTECHNOLOGY INSTITUTE
Inventors:
Jung-Seob KIM, Seongtae YUN, Heechun KWAK, Mee Sook OH, Sumin LEE
Abstract: Targeting constructs and methods of using them are provided for differentiation-dependent modification of nucleic acid sequences in cells and in non-human animals. Targeting constructs comprising a promoter operably linked to a recombinase are provided, wherein the promoter drives transcription of the recombinase in an differentiated cell but not an undifferentiated cell. Promoters include Blimp1, Prm1, Gata6, Gata4, Igf2, Lhx2, Lhx5, and Pax3. Targeting constructs with a cassette flanked on both sides by recombinase sites can be removed using a recombinase gene operably linked to a 3?-UTR that comprises a recognition site for an miRNA that is transcribed in undifferentiated cells but not in differentiated cells. The constructs may be included in targeting vectors, and can be used to automatically modify or excise a selection cassette from an ES cell, a non-human embryo, or a non-human animal.
Type:
Application
Filed:
July 1, 2016
Publication date:
October 20, 2016
Applicant:
Regeneron Pharmaceuticals, Inc.
Inventors:
David Frendewey, Guochun Gong, Ka-Man Venus Lai, David M. Valenzuela
Abstract: The present invention provides methods of achieving directed evolution of viruses by in vivo screening or “panning” to identify viruses comprising scrambled AAV capsids having characteristics of interest, e.g., tropism profile and/or neutralization profile (e.g., ability to evade neutralizing antibodies). The invention also provides scrambled AAV capsids and virus particles comprising the same.
Abstract: The present invention relates to a method for site-specific chromosomal integration of a gene library in a filamentous fungal host cell and a polynucleotide construct suitable for this purpose.
Type:
Application
Filed:
December 3, 2014
Publication date:
October 20, 2016
Inventors:
Bjarne G. Hansen, Carsten Lillelund Olsen, Jesper Vind
Abstract: Provided in the present invention is a method for positioning and integrating transgene and a use thereof. Specifically provided is a variant loxP element. The sequence “ATAAT” of an reverse repeated sequence in a wild type loxp locus is mutated into “CACCT”, i.e., a variant loxp element. Also provided in the present invention are a construct comprising the variant loxP element, a vector or host cell comprising the construct and a method for preparing a transgenic animal using the vector and the host cell.
Type:
Application
Filed:
September 28, 2013
Publication date:
October 20, 2016
Inventors:
Guoxiang CHENG, Jianquan CHEN, Siguo LIU
Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
Type:
Application
Filed:
February 12, 2016
Publication date:
October 20, 2016
Inventors:
Prashant G. Mali, George M. Church, Luhan Yang
Abstract: Ethanol is produced at high concentration by the fermentation of sugars using a biocatalyst comprising an open, porous hydrophilic polymeric structure having microorganisms for the bioconversion of sugar irreversibly retained therein in which the microorganisms have undergone phenotypic alteration to enhance tolerance to ethanol.
Abstract: Yeast cells with reduced activity of certain enzymes involved in branched chain amino acid biosynthesis in yeast mitochondria are described. Target enzymes include threonine deaminase, isopropylmalate synthase, and optionally branched chain amino acid transaminase.
Abstract: A truncated version of Saccharomyces cerevisiae IVL2 gene encoding a cytosolic form of acetolactate synthase was cloned into an expression cassette under control of a strong constitutive alcohol dehydrogenase (ADH1) promoter. The plasmid was introduced into the S. cerevisiae strain and the recombinant strain was tested for ability to overproduce glycerol under anaerobic conditions. It was shown that the recombinant strain was characterized by increased glycerol production and decreased ethanol production under anaerobic conditions.
Type:
Application
Filed:
December 5, 2014
Publication date:
October 20, 2016
Inventors:
Andriy Sibirny, Kostyantyn V Dmytruk, Charles Abbas, Lidiia R. Murashchenko
Abstract: This invention relates to a process for preparing succinate ester from a succinic acid salt present in a fermentation broth. In the first stage of this invention, renewable carbon resources are utilized to produce succinic acid in the form of a succinic acid salt through biological fermentation. The succinic acid salt present in the fermentation broth is subjected to double displacement reaction with a strong acid leading to the release of succinic acid. Succinic acid is recovered by fractional crystallization integrated with an alcohol washing step and subjected to esterification reaction to produce succinate ester which is purified by fractional distillation. The succinate ester thus obtained is converted into 1,4-butanediol, gamma-butyrolactone and tetrahydrofuran through hydrogenation reactions. The succinate ester can also be hydrolyzed to yield highly pure succinic acid.
Type:
Application
Filed:
December 5, 2014
Publication date:
October 20, 2016
Applicant:
Myriant Corporation
Inventors:
Thidarat Tosukhowong, Bin Wang, Manav Mistry, Rajesh Dasari, Zachary Wilson
Abstract: The invention relates to various new yeast strains of the type Yarrowia lipolytica as well as relevant methods for the biocatalytic preparation of ?-hydroxy fatty acids or dicarboxylic acids with the aid of these strains, whereby the formation of ?-hydroxy fatty acids or dicarboxylic acids is advantageously increased.
Type:
Application
Filed:
December 10, 2014
Publication date:
October 20, 2016
Inventors:
Michael GATTER, Falk MATTHAUS, Gerold BARTH
Abstract: The present disclosure relates to methods for producing microbial lipids. The present disclosure also relates to methods for producing microbial lipids using inhibitors obtainable from lignocellulosic materials to supress the proliferation of unwanted microorganisms in the fermentation broth. The method can therefore reduce the risk of having contaminating microbes establish in the system and the cultivation and thus higher yields of microbial lipids may be obtained.
Type:
Application
Filed:
December 11, 2014
Publication date:
October 20, 2016
Applicant:
NESTE OYJ
Inventors:
Heidi VAINIO, Mika SIPPONEN, Simo LAAKSO, Ossi PASTINEN, Ilkka LEHTOMÄKI, Heidi KAHELIN, Perttu KOSKINEN, Miia LAAMANEN
Abstract: A process for producing on an industrial scale the oxidized coenzyme Q10, includes culturing a reduced coenzyme Q10-producing microorganism selected from the group consisting of the genus Rhodobacter, the genus Saitoella, the genus Schizosaccharomyces and the genus Trichosporon, to obtain microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10; and one of: (a) oxidizing thus-obtained reduced coenzyme Q10 to oxidized coenzyme Q10 and then extracting the oxidized coenzyme Q10 by an organic solvent; or (b) extracting reduced coenzyme Q10 by an organic solvent and oxidizing the extracted reduced coenzyme Q10 to oxidized coenzyme Q10.
Abstract: The invention relates to genetically modified Pichia ciferrii cells, to the use thereof and to a method of producing sphingoid bases and sphingolipids.
Type:
Application
Filed:
June 27, 2016
Publication date:
October 20, 2016
Applicant:
EVONIK DEGUSSA GMBH
Inventors:
Tim Köhler, Christoph Schorsch, Eckhard Boles, Heiko Andrea, Mike Farwick, Steffen Schaffer
Abstract: The present invention relates to a modified microorganism having, compared to its wildtype, an increased activity of the enzyme that is encoded by the alaD-gene. The present invention also relates to a method for producing an alanine and to the use of modified microorganisms.
Type:
Application
Filed:
August 18, 2014
Publication date:
October 20, 2016
Applicant:
BASF SE
Inventors:
Joanna Martyna Krawczyk, Stefan Haefner, Hartwig Schröder, Oskar Zelder, Jonathan Thomas Fabarius
Abstract: The present invention relates to a polypeptide having a resistant to feedback inhibition by methionine and an activity of homoserine O-succinyltransferase, a O-succinyl homoserine-producing microorganism expressing the polypeptide, and a method for producing O-succinyl homoserine using the same.
Type:
Application
Filed:
October 22, 2014
Publication date:
October 20, 2016
Inventors:
Su Jin CHOI, So Young KIM, Chang Il SEO, Yong Uk SHIN, Young Lyeol YANG, Hye Won UM, Hye Min PARK, Sung Hoo JHON, Byung Hoon JUNG
Abstract: The present invention relates to a polypeptide having a resistant to feedback inhibition by methionine and an activity of homoserine O-succinyltransferase, a O-succinyl homoserine-producing microorganism expressing the polypeptide, and a method for producing O-succinyl homoserine using the same.
Type:
Application
Filed:
October 22, 2014
Publication date:
October 20, 2016
Inventors:
Su Jin CHOI, So Young KIM, Chang Il SEO, Yong Uk SHIN, Young Lyeol YANG, Hye Won UM, Hye Min PARK, Sung Hoo JHON, Byung Hoon JUNG
Abstract: The present invention relates to a polypeptide which is resistant to feedback inhibition by methionine and has an activity of homoserine O-succinyltransferase, a microorganism for producing O-succinylhomoserine which expresses the polypeptide, and a method for producing O-succinylhomoserine using the same.
Type:
Application
Filed:
October 22, 2014
Publication date:
October 20, 2016
Inventors:
Chang Il SEO, So Young KIM, Yong Uk SHIN, Kwang Ho NA, Hye Won UM, In Kyung HEO
Abstract: Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.
Type:
Application
Filed:
June 30, 2016
Publication date:
October 20, 2016
Inventors:
Steven J. Collier, Ee Ling Teo, Joly Sukumaran, Robert J. Wilson, Junye Xu
Abstract: The present invention provides for a genetically modified host cell comprising a 2-pyrrolidone synthase, or an enzymatically active fragment thereof, heterologous to the host cell.
Type:
Application
Filed:
April 14, 2016
Publication date:
October 20, 2016
Applicant:
The Regents of the University of California
Abstract: The present invention relates to host cells that produce compounds that are characterized as benzylisoquinolines, as well as select precursors and intermediates thereof. The host cells comprise one, two or more heterologous coding sequences wherein each of the heterologous coding sequences encodes an enzyme involved in the metabolic pathway of a benzylisoquinoline, or its precursors or intermediates from a starting compound. The invention also relates to methods of producing the benzylisoquinoline, as well as select precursors and intermediates thereof by culturing the host cells under culture conditions that promote expression of the enzymes that produce the benzylisoquinoline or precursors or intermediates thereof.
Abstract: Foods that are fermented with synergistic and simultaneous use of bacilli and mycelial cultures. The foods include a cellulosic food substrate containing detectable amounts of 1,3/1,6 ?-glucan which has been fermented by a bacilli strain of bacteria in cooperation with mycelium of a strain of Agaricomycetes fungi including polyporales. The bacilli strain maintains the food substrate at a pH of less than 4.7 to inhibit growth of pathogenic microbes and the consequent need to sterilize the food substrate in a sterile environment prior to fermentation by the mycelial culture.
Type:
Application
Filed:
April 13, 2016
Publication date:
October 20, 2016
Inventors:
Kevin Henry Fortin, John Favier, Mara Jane King
Abstract: Improved enzyme based methods of separating protein from protein-rich material are provided. A method can include utilizing a modeling equation to more effectively hydrolyze the various types of carbohydrates present in a protein-rich material. A method can include a fed-batch method of incrementally adding a protein-rich material, an enzyme broth, or both a protein-rich material and an enzyme broth. A method can also include partially or completely recycling the hydrolysate.
Abstract: The present invention is related to a method for adding one or more L-nucleotides to the 3?end of a first L-nucleic acid, wherein the method comprises the step of reacting the one or more L-nucleotides with the first L-nucleic acid in the presence of a protein comprising a mutant enzymatic activity exhibiting moiety, wherein the enzymatic activity is capable of adding one or more L-nucleotides to the 3? end of the first L-nucleic acid, wherein the mutant enzymatic activity exhibiting moiety comprises an amino acid sequence, wherein the amino acids of the amino acid sequence are D-amino acids, wherein the mutant enzymatic activity exhibiting moiety is a variant of an enzymatic activity exhibiting moiety, wherein the enzymatic activity exhibiting moiety consists of an amino acid sequence according to SEQ ID NO: 15 and wherein the amino acids of the amino acid sequence according to SEQ ID NO: 15 are D-amino acids, wherein the amino acid sequence of the mutant enzymatic activity exhibiting moiety differs from the
Type:
Application
Filed:
November 20, 2014
Publication date:
October 20, 2016
Applicant:
NOXXON Pharma AG
Inventors:
Andreas Pech, Florian Jarosch, Michael Jahnz, Sven Klussmann, Ralf David
Abstract: The invention provides a method of preparing a new recombinant antibacterial polypeptide medicine, which primarily relates to liquid culture medium formula suitable for large-scale production of the antibacterial polypeptide, as well as optimization of the enlarged culture parameters.
Type:
Application
Filed:
March 1, 2012
Publication date:
October 20, 2016
Applicant:
BEIJING CREATED TRIBIOTECHNOLOGY CO., LTD.
Abstract: The present invention pertains to a cell culture medium comprising media supplements that are shown to control recombinant protein glycosylation and/or cell culture in a controlled or modulated (shifted) temperature to control recombinant protein glycosylation and/or cell culture with controlled or modulated seed density to control recombinant protein glycosylation, and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.
Type:
Application
Filed:
January 19, 2016
Publication date:
October 20, 2016
Applicant:
Biogen MA Inc.
Inventors:
William YANG, Yao-Ming Huang, Kyle McElearney, Lia Tescione, James Lambropoulos, An Zhang, Valerie Tsang, Thomas Ryll
Abstract: A bio-enzyme sensor capable of super-hydrophobic solid-liquid-gas three-phase coexistence and a method for preparing the same. The bio-enzyme sensor comprises, from bottom to top, a base material with super-hydrophobic surface, a catalytic material having the function of catalyzing hydrogen peroxide, and bio-enzyme capable of reacting with a substance to be tested to generate hydrogen peroxide. A sufficient amount of oxygen can be provided for enzymatic reaction by forming a state of solid-liquid-gas three-phase coexistence on the surface of the super-hydrophobic material.
Abstract: An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained.
Type:
Application
Filed:
April 21, 2016
Publication date:
October 20, 2016
Applicant:
Ikeda Food Research Co., Ltd.
Inventors:
Takako YADA, Koji MIYAMOTO, Michinari HONDA
Abstract: The invention is directed towards methods and compositions for identifying the specific microorganisms present in a particular potion of a water process system. The method involves obtaining a sample from the process and determining if a problematic organism exceeds an acceptable threshold. If so a remedial bio-control procedure can be applied long before any unwanted defects or problems occur.
Abstract: The present invention refers to a method and a kit for the diagnosis of a hepatic disease utilizing the sulfydryl-oxidizing property of serum. Sulfydryl can be oxidized by serum, due to the QSOXs and other macromolecules or small molecules in serum. The specific activity of QSOXs in serum can be detected by fluorescent quantification using Amplex UltraRed (AUR), horseradish perioxidase, dithiothreitol, and tween as key reagents. The total dithiol oxidated capacity of the serum can be detected by colorimetric quantification using dithiothreitol, dithio-bis-nitrobenzoic acid, and guanidine hydrochloride as key reagents. The present invention shows that the specific activity of QSOXs in serum and the total dithiol oxidated capacity of the serum can be used for the diagnosis of hepatic disease, wherein the total dithiol oxidated capacity of the serum shows better diagnostic effect.
Abstract: Methods are provided for the prognosis, diagnosis and treatment of various pathological states, including cancer, chemotherapy resistance and dementia associated with Alzheimer's disease. The methods provided herein are based on the discovery that various proteins with a high level of sialylation are shown herein to be associated with disease states, such as, cancer, chemotherapy resistance and dementia associated with Alzheimer's disease. Such methods provide a lysosomal exocytosis activity profile comprising one or more values representing lysosomal exocytosis activity. Also provided herein, is the discovery that low lysosomal sialidase activity is associated with various pathological states. Thus, the methods also provide a lysosomal sialidase activity profile, comprising one or more values representing lysosomal sialidase activity. A lysosomal sialidase activity profile is one example of a lysosomal exocytosis activity profile.
Type:
Application
Filed:
June 20, 2016
Publication date:
October 20, 2016
Applicant:
St. Jude Children's Research Hospital
Inventors:
Ida Annunziata, Alessandra D'Azzo, Shai White-Gilbertson
Abstract: The present invention provides a method for measuring a modified nucleobase that increases detection sensitivity for the modified nucleobase in a target nucleic acid. Specifically, the present invention provides a method for measuring a modified nucleobase including: (1) incubating a nucleic acid sample, a capture probe, and a guide probe in a solution; and (2) measuring the modified nucleobase using an antibody against the modified nucleobase in the solution obtained at (1). The present invention also provides a kit for measuring a modified nucleobase including: (I) a guide probe; and (II) a capture probe and/or an antibody against the modified nucleobase.
Abstract: Provided herein are systems and methods for whole genome amplification and sequencing. In particular, provided herein are systems and methods for detection of nucleic acid variants (e.g., rare variants) in limited samples.
Type:
Application
Filed:
February 18, 2016
Publication date:
October 20, 2016
Inventors:
Alain-Albert Mir, Thomas David Schaal, Jude Dunne, Maithreyan Srinivasan
Abstract: Methods and reagents for performing digital PCR for detection and quantification of mutant alleles and copy number variation are disclosed. In particular, the invention relates to methods using a nonspecific DNA-binding dye, which produces a fluorescent signal that increases in intensity according to the number of base-pairs present in the PCR amplicon product. The method utilizes mutant-specific and wild-type-specific primers having non-complementary “tail” sequences of different lengths. Accordingly, the amplicons for the wild-type and mutant alleles differ in length and can be distinguished based on the difference in the intensities of their fluorescent signals. The methods of the invention can be used to detect rare genetic events, including single nucleotide mutations, alterations of copy number, and deletions or insertions of nucleotides.
Type:
Application
Filed:
December 16, 2014
Publication date:
October 20, 2016
Inventors:
Hanlee P. JI, Billy Tsz Cheong LAU, Laura MIOTKE
Abstract: Provided herein are methods and compositions for detecting gene fusions, e.g., relevant to cancer. The present methods and compositions can be used to detect gene fusions with very high sensitivity and specificity. The present methods and compositions can detect gene fusions, e.g., in free circulating tumor RNA from a plasma sample.
Type:
Application
Filed:
April 15, 2016
Publication date:
October 20, 2016
Inventors:
Ann Begovich, Rajiv Dua, Dwight Kuo, Xiaoju Max Ma, Ellen Ordinario
Abstract: The present invention relates to the field of RNA analysis. In particular, the invention concerns the use of a catalytic nucleic acid molecule for the analysis of an RNA molecule. The invention concerns methods for analyzing the 5? terminal structures of an RNA molecule having a cleavage site for a catalytic nucleic acid molecule.
Abstract: A method for scanning a microarray, including (a) providing a microarray to an optical scanner, wherein the microarray includes a surface having features having different target molecules; (b) scanning the surface in the x or y direction to acquire optical signals from the features at sequential regions of the surface, wherein the focus setting between the optical scanner and the surface is dynamically controlled in real time by repeatedly (i) adding a predetermined focal offset, thereby introducing an error in the focus setting, and (ii) adjusting the focus setting to reduce the error in the focus setting, thereby acquiring the optical signals from different regions at different degrees of focus such that a subset of the regions that are scanned have an introduced focus error; and (c) analyzing the optical signals that are obtained at the different degrees of focus to identify the different target molecules.
Type:
Application
Filed:
January 28, 2016
Publication date:
October 20, 2016
Applicant:
Illumina, Inc.
Inventors:
Darren Robert Segale, John A. Moon, Hongji Ren
Abstract: Methods of detecting target nucleic acid is a sample are described. A first probe is attached to first beads, and the first beads are placed in the sample so that any target nucleic acid attaches to the first probe. A second probe also attaches to the target nucleic acid so that any of the target nucleic acid links or “tethers” the first and second probes. A capacitive sensor detects capacitance of the beads and processes capacitance data to quantify target nucleic acid presence in the sample. The second probe may be immobilised on the sensor surface. Alternatively the second beads are introduced into the sample with the second probe attached, and the extent of tethering of the first beads to the second beads is indicative of the extent of target NA present.
Abstract: This disclosure relates to a method for increasing the hybridization efficiency of a probe and a target RNA in a sample, for example to identify a particular RNA present in the sample. The method includes heating a lysate sample comprising at least one target RNA, such as a tRNA, mRNA or rRNA, at a temperature of about 95° C. for a time sufficient to interfere with secondary structure of the RNA, wherein the time is short enough, such that the RNA in the cell lysate sample are not significantly degraded, and wherein the lysate comprises a cell lysis buffer comprising a chemical denaturant. To detect a target RNA in the lysate, the lysate is contacted with at least one detectable probe, such as a labeled probe, designed to specifically hybridize to the target RNA in the lysate.
Type:
Application
Filed:
December 5, 2014
Publication date:
October 20, 2016
Inventors:
Roby BHATTACHARYYA, Deb HUNG, Milesh PATEL
Abstract: Disclosed is a method of facilitating detection of a nucleic acid target sequence. The method may include obtaining a toehold-mediated DNA strand displacement apparatus comprising a portion complementary to the nucleic acid target sequence. The method may further include obtaining an RNA toehold switch comprising an RNA sequence comprising an inverted loop with a sequestered ribosome binding site and a sequestered translation start codon, a transducer sequence on the 3? side of the inverted loop, coding for a reporter protein, and a toehold portion on the 5? side of the inverted loop. Further, the toehold portion of the RNA sequence may be complementary to a portion of the Toehold-mediated DNA strand displacement apparatus. The method may further include combining the Toehold-mediated DNA strand displacement apparatus and the RNA toehold switch in an assay, such that the two are never in direct physical contact with each other.
Abstract: A method and kit for discriminating between breast cancer and benign breast disease by the determination of the expression level of at least one target gene having a nucleic acid sequence selected from the nucleic acid sequences set forth in SEQ ID NOs: 1, 2 or 3, 4 and 5 or 6 to obtain an expression profile for the patient, and the comparison of the expression profile of the patient with expression profiles of target genes from patients previously clinically classified as breast cancer and expression profiles of target genes from patients previously clinically classified as benign breast disease.
Type:
Application
Filed:
June 30, 2016
Publication date:
October 20, 2016
Applicant:
BIOMERIEUX
Inventors:
Xun YE, Fei WU, Qinghua XU, Xia MENG, Bruno MOUGIN
Abstract: The invention provides methods and compositions for separately denaturing a probe and target in hybridization applications. The invention may, for example, eliminate the use of or reduce the dependence on formamide in hybridization applications. Compositions for use in the invention include an aqueous composition comprising at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.
Abstract: Provided are methods of producing a product nucleic acid. The methods include combining a template deoxyribonucleic acid (DNA), a polymerase, a template switch oligonucleotide, and dNTPs into a reaction mixture. The components are combined into the reaction mixture under conditions sufficient to produce a product nucleic acid that includes the template DNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.
Abstract: This disclosure describes, in one aspect, a method of amplifying an RNA template. Generally, the method includes synthesizing an oligonucleotide from the RNA template, isolating at least a portion of the oligonucleotide, and subjecting the isolated product to treatment with an RNase and/or glycosylase.
Type:
Application
Filed:
December 2, 2014
Publication date:
October 20, 2016
Inventors:
Praveensingh B. Hajeri, Subree Subramanian
Abstract: Methods for adding sequence tags during amplification of a nucleic acid target are provided. The target is amplified using two sets of primers that are modified such that only tagged targets can ligate to hairpin adapters that protect them from subsequent exonuclease treatment. Methods of making circular nucleic acids are also provided. Specific overhangs are created by exonuclease trimming with a polymerase such that only desired targets can ligate to sticky ended hairpin adapters that protect them from subsequent exonuclease treatment. Compositions, kits, and systems related to or useful in the methods are also described.